Surveyor PDA Detector Lamp Board Upgrade...

Surveyor Plus

Surveyor PDA DetectorLamp BoardUpgrade Guide

60053-97110 Revision B January 2009

http://www.surveymonkey.com/s.aspx?sm=R7gKOvhLXn3NTkpK2BefHQ_3d_3d

© 2009 Thermo Fisher Scientific Inc. All rights reserved.

Surveyor and Xcalibur are registered trademarks and ChromQuest is a trademark of Thermo Fisher Scientific Inc. in the United States. Windows is a registered trademark of Microsoft Corporation in the United States and other countries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.

Release history: Revision A April 2005, Revision B January 2009

For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug Administration or other competent authorities.

WEEE Compliance

This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product. See www.thermo.com/WEEERoHS for further information on Thermo Fisher Scientific’s compliance with these Directives and the recyclers in your country.

WEEE Konformität

Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC erfüllen. Das Produkt ist durch folgendes Symbol gekennzeichnet:

Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise, die nützlich sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter www.thermo.com/WEEERoHS.

http://www.thermo.com/WEEERoHS




Conformité DEEE

Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant:

Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage d'informations sur la conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont disponibles sur www.thermo.com/WEEERoHS.



AVVERTENZA

trumento s de ntes de

al arse y limentacion nto sin sus o remueva

s tarjetas

Shock da folgorazione. L’apparecchio è alimentato da corrente ad alta tensione che puo provocare lesioni fisiche. Prima di effettuare qualsiasi intervento di manutenzione occorre spegnere ed isolare l’apparecchio dalla linea elettrica. Non attivare lo strumento senza lo schermo superiore. Non togliere i coperchi a protezione dalle schede di circuito stampato (PCB).

e contener . Utilice quimicos nos o cipientes y a

Prodotti chimici. Possibile presenza di sostanze chimiche pericolose nell’apparecchio. Indossare dei guanti per maneggiare prodotti chimici tossici, cancerogeni, mutageni, o corrosivi/irritanti. Utilizzare contenitori aprovo e seguire la procedura indicata per lo smaltimento dei residui di olio.

que lop de efectuar

Calore. Attendere che i componenti riscaldati si raffreddino prima di effetturare l’intervento di manutenzione.

ar el

Incendio. Adottare le dovute precauzioni quando si usa il sistema in presenza di gas infiammabili.

icaduras de s que usar teojos tos .

Pericolo per la vista. Gli schizzi di prodotti chimici o delle particelle presenti nell’aria potrebbero causare danni alla vista. Indossare occhiali protettivi quando si maneggiano prodotti chimici o si effettuano interventi di manutenzione sull’apparecchio.

e existe un gorias én se utiliza l usuario a n este

Pericolo generico. Pericolo non compreso tra le precedenti categorie. Questo simbolo è utilizzato inoltre sull’apparecchio per segnalare all’utente di consultare le istruzioni descritte nel presente manuale.

de un tes de o con la local para r Scientific

Quando e in dubbio la misura di sicurezza per una procedura, prima di continuare, si prega di mettersi in contatto con il Servizio di Assistenza Tecnica locale per i prodotti di Thermo Fisher Scientific San Jose.

CAUTION Symbol CAUTION VORSICHT ATTENTION PRECAUCION

Electric Shock: This instrument uses high voltages that can cause personal injury. Before servicing, shut down the instrument and disconnect the instrument from line power. Keep the top cover on while operating the instrument. Do not remove protective covers from PCBs.

Elektroschock: In diesem Gerät werden Hochspannungen verwendet, die Verletzungen verursachen können. Vor Wartungsarbeiten muß das Gerät abgeschaltet und vom Netz getrennt werden. Betreiben Sie Wartungsarbeiten nicht mit abgenommenem Deckel. Nehmen Sie die Schutzabdeckung von Leiterplatten nicht ab.

Choc électrique: L’instrument utilise des tensions capables d’infliger des blessures corprelles. L’instrument doit être arrêté et débranché de la source de courant avant tout intervention. Ne pas utiliser l’instrument sans son couvercle. Ne pas elensver les étuis protecteurs des cartes de circuits imprimés.

Descarga eléctrica: Este insutiliza altas tensiones, capaceproducir lesiones personales. Adar servicio de mantenimientoinstrumento, éste debera apagdesconectarse de la línea de aeléctrica. No opere el instrumecubiertas exteriores quitadas. Nlas cubiertas protectoras de lade circuito impreso.

Chemical: This instrument might contain hazardous chemicals. Wear gloves when handling toxic, carcinogenic, mutagenic, or corrosive or irritant chemicals. Use approved containers and proper procedures to dispose waste oil.

Chemikalien: Dieses Gerät kann gefährliche Chemikalien enthalten. Tragen Sie Schutzhandschuhe beim Umgang mit toxischen, karzinogenen, mutagenen oder ätzenden/reizenden Chemikalien. Entsorgen Sie verbrauchtes Öl entsprechend den Vorschriften in den vorgeschriebenen Behältern.

Chimique: Des produits chemiques dangereux peuven se trouver dans l’instrument. Proted dos gants pour manipuler tous produits chemiques toxiques, cancérigènes, mutagènes, ou corrosifs/irritants. Utiliser des récipients et des procédures homologuées pour se débarrasser des déchets d’huile.

Química: El instrumento puedproductos quimicos peligrososguantes al manejar productos tóxicos, carcinogenos, mutagecorrosivos/irritantes. Utilice reprocedimientos aprobados pardeshacerse del aceite usado.

Heat: Before servicing the instrument, allow any heated components to cool.

Hitze: Warten Sie erhitzte Komponenten erst nachdem diese sich abgekühlt haben.

Haute Temperature: Permettre aux composants chauffés de refroidir avant tout intervention.

Altas temperaturas: Permitacomponentes se enfríen, ante servicio de mantenimiento.

Fire: Use care when operating the system in the presence of flammable gases.

Feuer: Beachten Sie die einschlägigen VorsichtsmaBnahmen, wenn Sie das System in Gegenwart von entzündbaren Gasen betreiben.

Incendie: Agir avec précaution lors de l’utilisation du système en présence de gaz inflammables.

Fuego: Tenga cuidado al opersistema en presencia de gasesinflamables.

Eye Hazard: Eye damage could occur from splattered chemicals or flying particles. Wear safety glasses when handling chemicals or servicing the instrument.

Verletzungsgefahr der Augen: Verspritzte Chemikalien oder kleine Partikel können Augenverletzungen verursachen. Tragen Sie beim Umgang mit Chemikalien oder bei der Wartung des Gerätes eine Schutzbrille.

Danger pour les yeux: Dex projections chimiques, liquides, ou solides peuvent être dangereuses pour les yeux. Porter des lunettes de protection lors de toute manipulationde produit chimique ou pour toute intervention sur l’instrument.

Peligro par los ojos: Las salproductos químicos o particulasalten bruscamente pueden calesiones en los ojos. Utilice anprotectores al mnipular producquímicos o al darle servicio demantenimiento al instrumento

General Hazard: A hazard is present that is not included in the above categories. Also, this symbol appears on the instrument to refer the user to instructions in this manual.

Allgemeine Gefahr: Es besteht eine weitere Gefahr, die nicht in den vorstehenden Kategorien beschrieben ist. Dieses Symbol wird im Handbuch auBerdem dazu verwendet, um den Benutzer auf Anweisungen hinzuweisen.

Danger général: Indique la présence d;un risque n’appartenant pas aux catégories citées plus haut. Ce symbole figure également sur l’instrument pour renvoyer l’utilisateur aux instructions du présent manuel.

Peligro general: Significa qupeligro no incluido en las cateanteriores. Este simbolo tambien el instrumento par referir alas instrucciones contenidas emanual.

When the safety of a procedure is questionable, contact your local Technical Support organization for Thermo Fisher Scientific San Jose Products.

Wenn Sie sich über die Sicherheit eines Verfahrens im unklaren sind, setzen Sie sich, bevor Sie fortfahren, mit Ihrer lokalen technischen Unterstützungsorganisation für Thermo Fisher Scientific San Jose Produkte in Verbindung.

Si la sûreté d’un procédure est incertaine, avant de continuer, contacter le plus proche Service Clientèle pour les produits de Thermo Fisher Scientific San Jose.

Cuando la certidumbre acerca procedimiento sea dudosa, anproseguir, pongase en contactOficina de Asistencia Tecnica los productos de Thermo FisheSan Jose.

CAUTION Symbol CAUTION

Electric Shock: This instrument uses high voltages that can cause personal injury. Before servicing, shut down the instrument and disconnect the instrument from line power. Keep the top cover on while operating the instrument. Do not remove protective covers from PCBs.

Chemical: This instrument might contain hazardous chemicals. Wear gloves when handling toxic, carcinogenic, mutagenic, or corrosive or irritant chemicals. Use approved containers and proper procedures to dispose waste oil.

Heat: Before servicing the instrument, allow any heated components to cool.

Fire: Use care when operating the system in the presence of flammable gases.

Eye Hazard: Eye damage could occur from splattered chemicals or flying particles. Wear safety glasses when handling chemicals or servicing the instrument.

General Hazard: A hazard is present that is not included in the above categories. Also, this symbol appears on the instrument to refer the user to instructions in this manual.

When the safety of a procedure is questionable, contact your local Technical Support organization for Thermo Fisher Scientific San Jose Products.

C

Contents

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ixRelated Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ixSafety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ixGood Laboratory Practices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiContacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii

Chapter 1 Unpacking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Chapter 2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3Removing the Old Lamp Power Supply Board . . . . . . . . . . . . . . . . . . . . . . . . . . 3Installing the New Lamp Power Supply Board . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Chapter 3 Lamp Intensities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9Accessing the Attenuators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Viewing an Intensity Scan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Viewing an Intensity Scan from ChromQuest . . . . . . . . . . . . . . . . . . . . . . . . 10Viewing an Intensity Scan From Xcalibur . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Adjusting the Intensity of the Deuterium Lamp . . . . . . . . . . . . . . . . . . . . . . . . 15Changing the Settings of the SW1 Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Fine Tuning the Light Intensity after Changing the SW1 Switch

Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17Adjusting the Intensity of the Tungsten Lamp . . . . . . . . . . . . . . . . . . . . . . . . . 18

Adjusting the R48 Potentiometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18Fine Tuning the Light Intensity after Adjusting the R48 Potentiometer . . . . 20

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Thermo Scientific Surveyor PDA Detector Lamp Board Upgrade Guide vii

P

Preface

This installation guide describes how to upgrade the lamp power supply board for your Surveyor PDA Detector.

Related DocumentationRead the safety section before you install the components of the upgrade kit.

This installation guide contains the following chapters:

• Chapter 1, “Unpacking,” describes the contents of the Lamp Control Upgrade Kit.

• Chapter 2, “Installation,” shows you how to install the new lamp power supply board.

• Chapter 3, “Lamp Intensities,” shows you how to adjust the light intensities of the tungsten and deuterium lamps.

Safety and Special NoticesMake sure you follow the precautionary statements presented in this guide. The safety and other special notices appear in boxes.

Safety and special notices include the following:

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION notice is accompanied by an appropriate CAUTION symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of data, or invalid test results; or might contain information that is critical for optimal performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Thermo Scientific Surveyor PDA Detector Lamp Board Upgrade Guide ix

Preface

x

Every instrument has specific hazards, so be sure to read and comply with the following precautions. They will help ensure the safe, long-term use of your system.

1. Before plugging in any of the instrument modules or turning on the power, always make sure that the voltage and fuses are set appropriately for your local line voltage.

2. Only use fuses of the type and current rating specified. Do not use repaired fuses and do not short-circuit the fuse holder.

3. The supplied power cord must be inserted into a power outlet with a protective earth contact (ground). When using an extension cord, make sure that the cord also has an earth contact.

4. Do not change the external or internal grounding connections. Tampering with or disconnecting these connections could endanger you and/or damage the system.

5. Only a trained Thermo Fisher Scientific service representative is permitted to operate the detector without its housing. Permanent damage to the detector, as well as personal injury can occur by touching the internal components of the detector when it is connected to line power.

6. Do not turn the instrument on if you suspect that it has incurred any kind of electrical damage. Instead, disconnect the power cord and contact a service representative for a product evaluation. Do not attempt to use the instrument until it has been evaluated. (Electrical damage may have occurred if the system shows visible signs of damage, or has been transported under severe stress.)

7. Damage can also result if the instrument is stored for prolonged periods under unfavorable conditions (for example, subjected to heat, water, and so on).

8. Always disconnect the power cord before attempting any type of maintenance.

9. Capacitors inside the instrument might still be charged even if the instrument is turned off.

10. The procedures described in this manual that require the operation of the detector without its housing are only to be performed by a trained Thermo Fisher Scientific service representative.

CAUTION The instrument is properly grounded in accordance with regulations when shipped. You do not need to make any changes to the electrical connections or to the instrument’s chassis to ensure safe operation.

Surveyor PDA Detector Lamp Board Upgrade Guide Thermo Scientific

Preface

T

Good Laboratory Practices

Keep Good Records

To help identify and isolate problems with either your equipment or your methodology, we recommend that you keep good records of all system conditions (for example, % RSDs on retention times and peak areas, peak shape and resolution). At a minimum, keep a chromatogram of a typical sample and standard mixture, well documented with system conditions, for future reference. Careful comparison of retention times, peak shapes, peak sensitivity, and baseline noise can provide valuable clues to identifying and solving future problems.

Chemical Toxicity

Although the large volume of toxic and flammable solvents used and stored in laboratories can be quite dangerous, do not ignore the potential hazards posed by your samples. Take special care to read and follow all precautions that ensure proper ventilation, storage, handling, and disposal of both solvents and samples. Become familiar with the toxicity data and potential hazards associated with all chemicals by referring to the manufacturers’ Material Safety Data Sheets (MSDS).

Sample Preparation

Always consider the solubility of your sample in the solvent/mobile phase. Sample precipitation can plug the column, tubing and/or flowcell causing flow restriction. This obstruction may result in irreparable damage to the system. Particulate matter can be avoided by filtering the samples through 0.45 or 0.2 micron (or less) filters.

Solvent Requirements

Many chemical manufacturers provide a line of high-purity or HPLC-grade reagents that are free of chemical impurities. Routine filtration of all solvents or eluents through a 0.45 or 0.2 micron (or less) fluorocarbon filter before placing them in the solvent reservoir will significantly prolong the life and effectiveness of the inlet filters, check valves and seals, injector, and column. Typically, HPLC-grade solvents do not require filtration.

Choose a mobile phase that’s compatible with the sample and column you’ve selected for your separation. Remember that some solvents are corrosive to stainless steel. Inert, biocompatible versions of instruments are also available from Thermo Fisher Scientific.

hermo Scientific Surveyor PDA Detector Lamp Board Upgrade Guide xi

Preface

xii

Solvent Disposal

Make sure you have a solvent waste container or other kind of drain system available at or below the benchtop level. Most solvents have special disposal requirements and should not be disposed of directly down a drain. Follow all governmental regulations when disposing of any chemical.

High-Pressure Systems and Leaks

LC systems operate at high pressures. Because liquids are not highly compressible they do not store much energy. Accordingly, there is little immediate danger from the high pressures in an LC system. However, if a leak occurs, it should be corrected as soon as possible. Finally, we recommend that you always wear eye and skin protection when working on an LC system and that you always shut down the system and return it to atmospheric pressure before attempting any maintenance.


Preface

T

Contacting UsThere are several ways to contact Thermo Fisher Scientific for the information you need.

To contact Technical Support

Find software updates and utilities to download at mssupport.thermo.com.

To contact Customer Service for ordering information

To copy manuals from the Internet

Go to mssupport.thermo.com and click Customer Manuals in the left margin of the window.

To suggest changes to documentation or to Help

• Complete a brief survey about this document by clicking the link below. Thank you in advance for your help.

• Send an e-mail message to the Technical Publications Editor at [email protected].

Phone 800-532-4752

Fax 561-688-8736

E-mail [email protected]

Knowledge base www.thermokb.com

Phone 800-532-4752

Fax 561-688-8731

E-mail [email protected]

Web site www.thermo.com/ms

hermo Scientific Surveyor PDA Detector Lamp Board Upgrade Guide xiii

mailto:[email protected]

http://www.thermokb.com

http://mssupport.thermo.com

http://www.thermo.com/finnigan

http://mssupport.thermo.com

http://www.surveymonkey.com/s.aspx?sm=R7gKOvhLXn3NTkpK2BefHQ_3d_3d

mailto:[email protected]

1

Unpacking

The Lamp Control Upgrade kit contains the following items:

• This installation guide (P/N 60053-97110)

• The new lamp power supply board (P/N 60053-61035)

• A plastic tuning tool (P/N 00725-01-00007)

Thermo Scientific Surveyor PDA Detector Lamp Board Upgrade Guide 1

2

Installation

To upgrade the lamp control board for your Surveyor PDA detector, follow these procedures:

1. Removing the Old Lamp Power Supply Board

2. Installing the New Lamp Power Supply Board

Removing the Old Lamp Power Supply BoardTo remove the lamp power supply board

1. Turn off the power to the Surveyor PDA Detector and unplug it from line power.

2. Unplug the LED cable from the inside of the right door of the detector. See Figure 1.

Figure 1. Detaching the LED cable

CAUTION Because the detector contains a high voltage supply, you must turn off the power to the Surveyor PDA Detector and unplug it from line power before you pull the chassis out of its housing.

LED cable


2 InstallationRemoving the Old Lamp Power Supply Board

4

3. Using a #2 Phillips screwdriver, remove the four screws that secure the chassis of the detector to its housing. See Figure 2. Set the four screws aside.

Figure 2. Removing the screws that secure the housing to the chassis

4. Pull the chassis forward out of its housing.

CAUTION Because the detector contains a high voltage supply, you must turn off the power to the Surveyor PDA Detector and unplug it from line power before you pull the chassis out of the housing.

Four screws



5. Using a #2 Phillips head screwdriver, remove the two screws that secure the lamp power supply bracket (P/N F3028) to the detector. See Figure 3. Set the screws aside.

Figure 3. View of lamp power supply bracket

6. Disconnect the following cables attached to the lamp power supply board (see Figure 4 and Figure 5):

• Deuterium lamp cable

• Tungsten lamp cable

• Power supply control cable

• Ground wire

• Transformer cable

Screw securing brackets

Lamp power supply bracket

LED cable



6

Figure 4. Cables attached to the back of the lamp power supply board

Figure 5. Deuterium lamp and tungsten lamp cables

7. Lift the lamp power supply board out of its guides.

Power supply control cable

Groundwire

Transformer cable

Deuterium lampcable

Tungsten lampcable


2 InstallationInstalling the New Lamp Power Supply Board

Installing the New Lamp Power Supply BoardTo install the new lamp power supply board

1. Slide the lamp power supply board (P/N 60053-61035) into the guide rails. Take care not to damage the cables going across the floor of the detector. Ensure that these cables are not pinched by board or by the heatsink on the board.

2. Place the LED cable across the top of the lamp power supply board.

3. Reinstall the lamp power supply bracket (P/N F3028). Secure the bracket with the two screws that you set aside in step 5 on page 5.

4. Reconnect the following cables to the new lamp power supply board (see Figure 4 and Figure 5):

• Deuterium lamp cable

• Tungsten lamp cable

• Power supply control cable

• Ground wire

• Transformer cable

5. Slide the chassis back into the housing.

6. Using the four screws that you set aside in step 3 on page 4, secure the chassis to the housing. See Figure 2 on page 4.

7. Reconnect the LED cable to the receptacle inside the right door of the detector. See Figure 1 on page 3.

You have successfully installed the new lamp power supply board. If you want to customize the lamp intensities for a particular application, see Chapter 3, “Lamp Intensities.”

CAUTION The cables on the floor of the detector’s chassis are easy to damage. Take care not to damage these cables as you install the new lamp control board.


3

Lamp Intensities

After you install the lamp power supply board, check the intensity scan for the lamps from the data system that controls the detector. With the attenuators set at 25% of the full open position, the maximum intensities in the UV and Visible regions should be between 600000 and 900000 intensity counts.

If the maximum intensities of the lamps are not within these values, adjust the intensities by performing the procedures provided in this chapter.

To make a rough adjustment to the intensity of the deuterium lamp, you adjust the SW1 levers on the lamp power supply board. To make a rough adjustment to the intensity of the tungsten lamp, you adjust the R48 potentiometer on the lamp power supply board.

The light outputs for both lamps are fine tuned with the attenuator tabs that are located behind the flowcell access cover.

To adjust the intensities of the lamps, follow these procedures:

1. Accessing the Attenuators

2. Viewing an Intensity Scan

3. Adjusting the Intensity of the Deuterium Lamp

4. Adjusting the Intensity of the Tungsten Lamp

Accessing the AttenuatorsThe Surveyor PDA Plus Detector has two attenuators that control the light output from the lamps. Before you view the intensities of the lamps from the data system display, ensure that the attenuators are set to 25% of the full open position. Figure 6 shows the attenuators at 25% of the full open position.

To access the attenuators

1. Open the front doors of the detector.

2. Remove the flowcell cover by unscrewing the captive screw that secures it to the detector.

The attenuators are located on the right side of the front panel. There are two black tabs attached to the attenuators for manual adjustments.


3 Lamp IntensitiesViewing an Intensity Scan

10

The left tab is the deuterium lamp attenuator and the right tab is the tungsten lamp attenuator. Pushing an attenuator tab up increases light output and pulling an attenuator tab down decreases light output.

Figure 6. Attenuators set to 25% of the full open position

Viewing an Intensity ScanTo determine the light output for the lamps, you can view an intensity scan from the data system that controls the detector.

This section contains the following topics:

• Viewing an Intensity Scan from ChromQuest

• Viewing an Intensity Scan From Xcalibur

Viewing an Intensity Scan from ChromQuest

To view an intensity scan from the ChromQuest data system

1. Open the Diagnostics dialog box for the PDA detector:

a. From the Windows® XP taskbar, choose Start > All Programs > Chromatography > ChromQuest to open ChromQuest.

b. In the ChromQuest Main Menu, double-click the icon for your instrument to display the Instrument window.

c. From the menu bar, choose Control > Instrument Status to display the Instrument Status window.

Tungsten tab

Deuterium tab



T

d. In the Instrument Status window, click the Surveyor PDA (Plus) tab to display the Surveyor PDA (Plus) Instrument Status page.

e. Click Diagnostics to open the Surveyor PDA (Plus) Diagnostics dialog box.

2. Turn both lamps on:

a. Click the Lamps tab.

b. Record the usage hours for each lamp.

c. Verify that both lamps are On. If they are not On, click On for both lamps.

3. After both lamps are On, set the parameters for the spectral display of the lamp intensities:

a. Click the Control tab to display the Control page.

b. In the Mode area, select the Intensity option.

c. Click Default, and then verify that the following parameters are specified:

Start = 2

End = 511

Step = 1

Bandwidth = 1

Scan rate = 1

d. Click Load To Detector.

4. Determine the diode of maximum intensity for the deuterium lamp:

a. Click the Display tab to open the Display page.

b. Click Start located in the upper right corner of the Display page to start the data stream.

c. Right-click in the intensity spectrum to open a shortcut menu.

d. From the shortcut menu, choose Axis Setup to open the Axis Properties dialog box. See Figure 7.

e. Select X-axis and the Use this range option. Enter a Min value of 30 and a Max value of 50 for the diode range. Click OK to close the dialog box and update the scaling of the Spectrum view.

hermo Scientific Surveyor PDA Detector Lamp Board Upgrade Guide 11


12

Figure 7. Axis Properties dialog box

f. From the spectrum displayed, determine the pixel of maximum intensity within the 30 to 50 diode range. This is the diode of maximum output for the deuterium lamp.

5. Determine the diode of maximum intensity for the tungsten lamp (Visible range):

a. Access the Axis Setup dialog box a second time. Enter a Min value of 400 and a Max value of 500 for the diode range. Then, click OK to close the dialog box and update the scaling of the Spectrum view.

b. From the spectrum displayed, determine and record the pixel of maximum intensity within the 400 to 500 diode range. This is the diode of maximum output for the tungsten lamp.

c. Click Off for Status and Stop for Data to turn off the data stream.

6. Set the discrete channel displays:

a. Return to the Control page.

b. Type the value for the diode of maximum intensity for the deuterium lamp in the Channel A text box.

c. Type the value for the diode of maximum intensity for the tungsten lamp in the Channel C text box.

d. Click Load to Detector.

7. Return to the Display page and start the data stream.



T

8. Monitor the intensities values for the deuterium and tungsten lamp maximas:

• If the maximum intensity in both the UV region and the Visible region of the scan is between 600000 to 900000 counts, go to “Fine Tuning the Light Intensity after Adjusting the R48 Potentiometer” on page 20.

• If the maximum intensity in the UV region is not between 600000 to 900000 counts, go to “Adjusting the Intensity of the Deuterium Lamp” on page 15.

• If the maximum intensity in the UV region is between 600000 to 900000, but the maximum intensity in Visible region is not between 600000 to 900000 counts, go to “Adjusting the Intensity of the Tungsten Lamp” on page 18.

Viewing an Intensity Scan From Xcalibur

To view an intensity scan from the Xcalibur data system

1. Open the Instrument Setup view:

a. From the Windows XP taskbar, select Start > All Programs > Xcalibur > Xcalibur to open Xcalibur.

b. In the Xcalibur Home Page, click the Instrument Setup icon to display the Instrument Setup window.

2. Set your chromatographic pump to deliver HPLC-grade methanol at a flow rate of 1 mL/min:

a. In the view bar of the Instrument Setup view, click the Surveyor MS Pump or the Surveyor LC Pump icon to display the instrument setup view for your pump.

b. Choose Surveyor MS pump > Direct Control or Surveyor LC pump > Direct Control. The Direct Control dialog box for your pump appears.

c. Select a solvent bottle that contains HPLC-grade methanol and set the flow rate to 1 mL/min.

3. Create the .spda method for the Surveyor PDA Plus Detector:

a. In the view bar of the Instrument Setup view, click the Surveyor PDA Plus icon to display the Surveyor PDA Plus Method page.

b. In the Units area, select the Diode/Intensity option.

This selection sets the Y-axis of the spectra to intensity and the X-axis to diode number on the Display page of the Direct Control dialog box.



14

c. Verify that the following parameters are specified in the Spectra area of the Surveyor PDA Plus Method page:

Start Diode= 2End Diode= 511Step = 1Sample rate = 1Bandwidth = 1

d. Choose File > Save to save the method. Save the method with a filename such as attenuator.spda.

4. Make sure that the lamps are On:

a. Choose Surveyor PDA Plus > Direct Control. The Surveyor PDA Direct Control dialog box appears.

b. Click the Configuration tab to display the Configuration page.

c. Verify that both lamps are On. If they are not On, click Turn On for both lamps.

5. Load the .spda method to the detector:

a. In the Surveyor PDA Plus Direct Control dialog box, click the Display tab to open the Display page.

b. In the Control area, click Load Method and choose your attenuator method file from the list.

c. Click OK.

6. In the Control area, click Start. The spectrum of light intensities appears in the top window.

For the UV region, the diode of maximum intensity is between diode 30 and diode 50. For the Visible region, the diode of maximum intensity is between diode 400 and diode 500. Ignore the spike at diode number 380 (approx.). This spike is an emission line of the D2 lamp.

Note The .spda file extension is a special file extension used for all method files based on Diode/Intensity units. These methods cannot be used in Sequence Setup. Files with the .spda extension can only be loaded and used in the Direct Control dialog box.


3 Lamp IntensitiesAdjusting the Intensity of the Deuterium Lamp

T

7. Monitor the intensities in both the UV and Visible region of the scan:

• If the maximum intensity in both the UV region and the Visible region of the scan is between 600000 to 900000 counts, go to “Fine Tuning the Light Intensity after Adjusting the R48 Potentiometer” on page 20.

• If the maximum intensity in the UV region is not between 600000 to 900000 counts, go to “Adjusting the Intensity of the Deuterium Lamp” on page 15.

• If the maximum intensity in the UV region is between 600000 to 900000, but the maximum intensity in Visible region is not between 600000 to 900000 counts, go to “Adjusting the Intensity of the Tungsten Lamp” on page 18.

Adjusting the Intensity of the Deuterium LampIf the maximum intensity in the UV region is not between 600000 to 900000 counts, you need to change the settings of the SW1 switch before you fine tune the light output with the attenuators.


• Changing the Settings of the SW1 Switch

• Fine Tuning the Light Intensity after Changing the SW1 Switch Settings

Changing the Settings of the SW1 Switch

Increasing the current increases the intensity of the deuterium lamp. The current settings, which are adjusted by pushing the levers of the SW1 switch (see Figure 8), are shown on the back of the board. See Figure 9.

The default setting is 125 mA.

To adjust the intensity in the UV region by changing the SW1 settings

1. Determine the maximum intensity in the UV region. Then increase or decrease the current:

• Increase the current if the maximum intensity in the UV region is less than 600000 intensity counts.

• Decrease the current if the maximum intensity in the UV region is greater than 900000 intensity counts.

CAUTION Use the plastic tuning tool supplied in this upgrade kit to adjust the levers of the SW1 switch. Never use a tool that conducts current when working on components inside the detector.



16

2. Using the plastic tuner, set the SW1 (see Figure 8 and Figure 9) switches up or down:

• Push the lever away from the PCB to set it to the “0” position.

• Push the lever towards the PCB to set it to the “1” position.

Figure 8. Switch settings

Figure 9. Current settings for the deuterium lamp

3. After you adjust the SW1 switch, go to the next procedure “Fine Tuning the Light Intensity after Adjusting the R48 Potentiometer.”

125 mA (default setting)

175 mA 250 mA 300 mA



T

Fine Tuning the Light Intensity after Changing the SW1 Switch Settings

The Surveyor PDA Plus Detector has two attenuators that control the light output from the lamps. During the lifetime of the Surveyor PDA Plus Detector, it might be necessary to adjust the attenuators to increase or decrease the amount of light falling onto the array. The attenuators require adjustment when either lamp is replaced or when the flowcell is replaced.

Decreasing the light output increases baseline noise. Increasing the light output can cause saturation of the diode array. If the array is saturated, the response from the Surveyor PDA Plus Detector is a flat baseline.

For optimal performance, the maximum intensity values in the UV region and the Visible should be between 750000 to 775000 intensity counts.

To adjust the maximum intensity values

1. Adjust the left attenuator tab (UV attenuation) to achieve a maximum value from 750000 to 775000 intensity counts in the region between diode number 30 and diode number 50.

2. Check the intensity of the scan in the Visible region:

• If the maximum intensity in the Visible region is between 600000 to 900000 counts, adjust the attenuator with the right tab (Visible attenuation) to achieve a maximum value from 750000 to 775000 intensity counts in the region between diode number 400 and diode number 500. See Figure .

Figure 10. Intensity scan performed from the ChromQuest data system

• If the maximum intensity in the Visible region is not between 600000 to 900000 counts, go to “Adjusting the Intensity of the Tungsten Lamp” on page 18.

3. After you finish adjusting the attenuators, replace the flowcell access cover and close the front doors of the detector.

UV region maximum

Visible regionmaximum


3 Lamp IntensitiesAdjusting the Intensity of the Tungsten Lamp

18

Adjusting the Intensity of the Tungsten LampIf the maximum intensity in the Visible region is not between 600000 to 900000 counts, you need to adjust the R48 potentiometer before you fine tune the light output with the right attenuator tab.


• Adjusting the R48 Potentiometer

• Fine Tuning the Light Intensity after Adjusting the R48 Potentiometer

Adjusting the R48 Potentiometer

To adjust the intensity of the tungsten lamp with the R48 potentiometer

Using the plastic tuner (P/N 00725-01-00007) supplied in this upgrade kit, turn the R48 potentiometer (see Figure 11 and Figure 12):

• Turn the R48 potentiometer clockwise to increase the intensity of the tungsten lamp.

• Turn the R48 potentiometer counterclockwise to decrease the intensity of the tungsten lamp.

For instructions on viewing the light intensity from your data system, see “Viewing an Intensity Scan from ChromQuest” on page 10 or “Viewing an Intensity Scan From Xcalibur” on page 13.

CAUTION Use the plastic tuning tool supplied in this upgrade kit to adjust the potentiometer. Never use a tool that conducts current when working on components inside the detector.



T

Figure 11. View of the R48 potentiometer on the lamp power supply board

Figure 12. Using the plastic tuner to adjust the R48 potentiometer

R48 potentiometer

SW1 switches

Plastic tuning tool

R48 potentiometer



20

Fine Tuning the Light Intensity after Adjusting the R48 Potentiometer

To fine tune the light intensity that reaches the diode array

1. Check the intensity of the scan in the Visible region:

• If the maximum intensity in the Visible region is not between 600000 to 900000 counts, adjust the R48 potentiometer before you fine tune the light output with the right attenuator tab.

• If the maximum intensity in the Visible region is between 600000 to 900000 counts, adjust the right attenuator to achieve a maximum value from 750000 to 775000 intensity counts in the region between diode number 400 and diode number 500. See Figure 10 on page 17.

1. Re-check the intensity of the scan in the UV region and adjust the left attenuator to achieve a maximum value from 750000 to 775000 intensity counts in the region between diode number 30 and diode number 50.

2. After you finish adjusting the attenuators, replace the flowcell access cover and close the front doors.


I

Index

Aattenuators (figure) 10

Ccables connected to the lamp power supply 5caution

high voltage supply 3current settings for the deuterium lamp (figure) 16

Ddeuterium lamp, adjusting the intensity 15

Llamp power supply board

installing 7removing 3

LED cable 3

Mmaximum intensity counts 17, 20

Pparts 1

RR48 potentiometer (figure) 19

Sscrews

securing the chassis to the housing 4securing the lamp power supply bracket 5

SW1 switch (figure) 16, 19

Ttuner used to adjust potentiometer (figure) 19tungsten lamp, adjusting the intensity 18


Surveyor PDA Detector Lamp Board Upgrade...

Documents

Transcript of Surveyor PDA Detector Lamp Board Upgrade...