Supporting Information for Proteomics DOI … · Supporting Information for Proteomics DOI 10 ... 8...

Supporting Information

for Proteomics

DOI 10.1002/pmic.200701057

Gianluca Picariello, Pasquale Ferranti, Gianfranco Mamone, Peter Roepstorff

and Francesco Addeo

Identification of N-linked glycoproteins in human milk by hydrophilic

interaction liquid chromatography and mass spectrometry

ª 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.proteomics-journal.com

In this file, almost all the MS/MS spectra of the formerly glycosylated peptides, identified by MASCOT search engine, are reported (see Table 1). Asparagine deamidation localizes the N-glycosylation sites. A few examples of identification by the PHENYX search engine are also shown. LACTOTRANSFERRIN (P02788)

# b b++ b* b*++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #

1 102.0550 51.5311 84.0444 42.5258 T 30

2 173.0921 87.0497 155.0815 78.0444 A 3130.6473 1565.8273 3113.6207 1557.3140 3112.6367 1556.8220 29

3 230.1135 115.5604 212.1030 106.5551 G 3059.6101 1530.3087 3042.5836 1521.7954 3041.5996 1521.3034 28

4 416.1928 208.6001 398.1823 199.5948 W 3002.5887 1501.7980 2985.5621 1493.2847 2984.5781 1492.7927 27

5 530.2358 265.6215 513.2092 257.1082 512.2252 256.6162 N 2816.5094 1408.7583 2799.4828 1400.2450 2798.4988 1399.7530 26

6 629.3042 315.1557 612.2776 306.6425 611.2936 306.1504 V 2702.4664 1351.7369 2685.4399 1343.2236 2684.4559 1342.7316 25

7 726.3570 363.6821 709.3304 355.1688 708.3464 354.6768 P 2603.3980 1302.2027 2586.3715 1293.6894 2585.3875 1293.1974 24

8 839.4410 420.2241 822.4145 411.7109 821.4304 411.2189 I 2506.3453 1253.6763 2489.3187 1245.1630 2488.3347 1244.6710 23

9 896.4625 448.7349 879.4359 440.2216 878.4519 439.7296 G 2393.2612 1197.1342 2376.2347 1188.6210 2375.2506 1188.1290 22

10 997.5102 499.2587 980.4836 490.7454 979.4996 490.2534 T 2336.2397 1168.6235 2319.2132 1160.1102 2318.2292 1159.6182 21

11 1110.5942 555.8007 1093.5677 547.2875 1092.5837 546.7955 L 2235.1921 1118.0997 2218.1655 1109.5864 2217.1815 1109.0944 20

12 1266.6953 633.8513 1249.6688 625.3380 1248.6848 624.8460 R 2122.1080 1061.5576 2105.0814 1053.0444 2104.0974 1052.5524 19

13 1363.7481 682.3777 1346.7215 673.8644 1345.7375 673.3724 P 1966.0069 983.5071 1948.9803 974.9938 1947.9963 974.5018 18

14 1510.8165 755.9119 1493.7900 747.3986 1492.8059 746.9066 F 1868.9541 934.9807 1851.9276 926.4674 1850.9436 925.9754 17

15 1623.9006 812.4539 1606.8740 803.9407 1605.8900 803.4486 L 1721.8857 861.4465 1704.8592 852.9332 1703.8751 852.4412 16

16 1738.9275 869.9674 1721.9010 861.4541 1720.9169 860.9621 N 1608.8016 804.9045 1591.7751 796.3912 1590.7911 795.8992 15

17 1925.0068 963.0070 1907.9803 954.4938 1906.9963 954.0018 W 1493.7747 747.3910 1476.7482 738.8777 1475.7641 738.3857 14

18 2026.0545 1013.5309 2009.0280 1005.0176 2008.0439 1004.5256 T 1307.6954 654.3513 1290.6688 645.8381 1289.6848 645.3461 13

19 2083.0760 1042.0416 2066.0494 1033.5283 2065.0654 1033.0363 G 1206.6477 603.8275 1189.6212 595.3142 1188.6371 594.8222 12

20 2180.1287 1090.5680 2163.1022 1082.0547 2162.1182 1081.5627 P 1149.6263 575.3168 1132.5997 566.8035 1131.6157 566.3115 11

21 2277.1815 1139.0944 2260.1549 1130.5811 2259.1709 1130.0891 P 1052.5735 526.7904 1035.5469 518.2771 1034.5629 517.7851 10

22 2406.2241 1203.6157 2389.1975 1195.1024 2388.2135 1194.6104 E 955.5207 478.2640 938.4942 469.7507 937.5102 469.2587 9

23 2503.2769 1252.1421 2486.2503 1243.6288 2485.2663 1243.1368 P 826.4781 413.7427 809.4516 405.2294 808.4676 404.7374 8

24 2616.3609 1308.6841 2599.3344 1300.1708 2598.3504 1299.6788 I 729.4254 365.2163 712.3988 356.7030 711.4148 356.2110 7

25 2745.4035 1373.2054 2728.3770 1364.6921 2727.3929 1364.2001 E 616.3413 308.6743 599.3148 300.1610 598.3307 299.6690 6

26 2816.4406 1408.7239 2799.4141 1400.2107 2798.4301 1399.7187 A 487.2987 244.1530 470.2722 235.6397 5

27 2887.4777 1444.2425 2870.4512 1435.7292 2869.4672 1435.2372 A 416.2616 208.6344 399.2350 200.1212 4

28 2986.5462 1493.7767 2969.5196 1485.2634 2968.5356 1484.7714 V 345.2245 173.1159 328.1979 164.6026 3

29 3057.5833 1529.2953 3040.5567 1520.7820 3039.5727 1520.2900 A 246.1561 123.5817 229.1295 115.0684 2

30 R 175.1190 88.0631 158.0924 79.5498 1


1 157.1084 79.0578 140.0818 70.5446 R 31

2 258.1561 129.5817 241.1295 121.0684 240.1455 120.5764 T 3231.6949 1616.3511 3214.6684 1607.8378 3213.6844 1607.3458 30

3 329.1932 165.1002 312.1666 156.5870 311.1826 156.0949 A 3130.6473 1565.8273 3113.6207 1557.3140 3112.6367 1556.8220 29

4 386.2146 193.6110 369.1881 185.0977 368.2041 184.6057 G 3059.6101 1530.3087 3042.5836 1521.7954 3041.5996 1521.3034 28

5 572.2940 286.6506 555.2674 278.1373 554.2834 277.6453 W 3002.5887 1501.7980 2985.5621 1493.2847 2984.5781 1492.7927 27

6 686.3369 343.6721 669.3103 335.1588 668.3263 334.6668 N 2816.5094 1408.7583 2799.4828 1400.2450 2798.4988 1399.7530 26

7 785.4053 393.2063 768.3787 384.6930 767.3947 384.2010 V 2702.4664 1351.7369 2685.4399 1343.2236 2684.4559 1342.7316 25

8 882.4581 441.7327 865.4315 433.2194 864.4475 432.7274 P 2603.3980 1302.2027 2586.3715 1293.6894 2585.3875 1293.1974 24

9 995.5421 498.2747 978.5156 489.7614 977.5316 489.2694 I 2506.3453 1253.6763 2489.3187 1245.1630 2488.3347 1244.6710 23

10 1052.5636 526.7854 1035.5370 518.2722 1034.5530 517.7802 G 2393.2612 1197.1342 2376.2347 1188.6210 2375.2506 1188.1290 22

11 1153.6113 577.3093 1136.5847 568.7960 1135.6007 568.3040 T 2336.2397 1168.6235 2319.2132 1160.1102 2318.2292 1159.6182 21

12 1266.6953 633.8513 1249.6688 625.3380 1248.6848 624.8460 L 2235.1921 1118.0997 2218.1655 1109.5864 2217.1815 1109.0944 20

13 1422.7964 711.9019 1405.7699 703.3886 1404.7859 702.8966 R 2122.1080 1061.5576 2105.0814 1053.0444 2104.0974 1052.5524 19

14 1519.8492 760.4282 1502.8227 751.9150 1501.8386 751.4230 P 1966.0069 983.5071 1948.9803 974.9938 1947.9963 974.5018 18

15 1666.9176 833.9624 1649.8911 825.4492 1648.9071 824.9572 F 1868.9541 934.9807 1851.9276 926.4674 1850.9436 925.9754 17

16 1780.0017 890.5045 1762.9751 881.9912 1761.9911 881.4992 L 1721.8857 861.4465 1704.8592 852.9332 1703.8751 852.4412 16

17 1895.0286 948.0179 1878.0021 939.5047 1877.0181 939.0127 N 1608.8016 804.9045 1591.7751 796.3912 1590.7911 795.8992 15

18 2081.1079 1041.0576 2064.0814 1032.5443 2063.0974 1032.0523 W 1493.7747 747.3910 1476.7482 738.8777 1475.7641 738.3857 14

19 2182.1556 1091.5814 2165.1291 1083.0682 2164.1450 1082.5762 T 1307.6954 654.3513 1290.6688 645.8381 1289.6848 645.3461 13

20 2239.1771 1120.0922 2222.1505 1111.5789 2221.1665 1111.0869 G 1206.6477 603.8275 1189.6212 595.3142 1188.6371 594.8222 12

21 2336.2298 1168.6186 2319.2033 1160.1053 2318.2193 1159.6133 P 1149.6263 575.3168 1132.5997 566.8035 1131.6157 566.3115 11

22 2433.2826 1217.1449 2416.2561 1208.6317 2415.2720 1208.1397 P 1052.5735 526.7904 1035.5469 518.2771 1034.5629 517.7851 10

23 2562.3252 1281.6662 2545.2986 1273.1530 2544.3146 1272.6610 E 955.5207 478.2640 938.4942 469.7507 937.5102 469.2587 9

24 2659.3780 1330.1926 2642.3514 1321.6793 2641.3674 1321.1873 P 826.4781 413.7427 809.4516 405.2294 808.4676 404.7374 8

25 2772.4620 1386.7347 2755.4355 1378.2214 2754.4515 1377.7294 I 729.4254 365.2163 712.3988 356.7030 711.4148 356.2110 7

26 2901.5046 1451.2559 2884.4781 1442.7427 2883.4941 1442.2507 E 616.3413 308.6743 599.3148 300.1610 598.3307 299.6690 6

27 2972.5417 1486.7745 2955.5152 1478.2612 2954.5312 1477.7692 A 487.2987 244.1530 470.2722 235.6397 5

28 3043.5788 1522.2931 3026.5523 1513.7798 3025.5683 1513.2878 A 416.2616 208.6344 399.2350 200.1212 4

29 3142.6473 1571.8273 3125.6207 1563.3140 3124.6367 1562.8220 V 345.2245 173.1159 328.1979 164.6026 3

30 3213.6844 1607.3458 3196.6578 1598.8326 3195.6738 1598.3405 A 246.1561 123.5817 229.1295 115.0684 2

31 R 175.1190 88.0631 158.0924 79.5498 1

POLYMERIC IG RECEPTOR (P01833)

FROM MALDI-TOF/TOF MS/MS Fragmentation of ANLTNFPENGTFVVNIAQLSQDDSGR Found in PIGR_HUMAN, Polymeric immunoglobulin receptor precursor - Homo sapiens (Human)

Monoisotopic mass of neutral peptide Mr(calc): 2808.3202 Fixed modifications: Carbamidomethyl (C) Variable modifications: N2 : Deamidated (NQ) N9 : Deamidated (NQ) Ions Score: 80 Expect: 9.2e-07 Matches (Bold Red): 30/497 fragment ions using 68 most intense peaks # Immon. a a* a0 b b* b0 Seq. v w w' y y* y0 #

1 44.0495 44.0495 72.0444 A 26

2 88.0393 159.0764 142.0499 187.0713 170.0448 N 2678.2693 2677.2740 2738.2904 2721.2638 2720.2798 25

3 86.0964 272.1605 255.1339 300.1554 283.1288 L 2565.1852 2564.1899 2623.2634 2606.2369 2605.2529 24

4 74.0600 373.2082 356.1816 355.1976 401.2031 384.1765 383.1925 T 2464.1375 2477.1579 2479.1372 2510.1794 2493.1528 2492.1688 23

5 87.0553 487.2511 470.2245 469.2405 515.2460 498.2194 497.2354 N 2350.0946 2349.0993 2409.1317 2392.1052 2391.1211 22

6 120.0808 634.3195 617.2929 616.3089 662.3144 645.2879 644.3038 F 2203.0262 2295.0888 2278.0622 2277.0782 21

7 70.0651 731.3723 714.3457 713.3617 759.3672 742.3406 741.3566 P 2105.9734 2104.9782 2148.0204 2130.9938 2130.0098 20

8 102.0550 860.4149 843.3883 842.4043 888.4098 871.3832 870.3992 E 1976.9308 1975.9356 2050.9676 2033.9410 2032.9570 19

9 88.0393 975.4418 958.4152 957.4312 1003.4367 986.4102 985.4261 N 1861.9039 1860.9086 1921.9250 1904.8985 1903.9144 18

10 30.0338 1032.4633 1015.4367 1014.4527 1060.4582 1043.4316 1042.4476 G 1806.8981 1789.8715 1788.8875 17

11 74.0600 1133.5109 1116.4844 1115.5004 1161.5058 1144.4793 1143.4953 T 1703.8347 1716.8551 1718.8344 1749.8766 1732.8501 1731.8660 16

12 120.0808 1280.5793 1263.5528 1262.5688 1308.5743 1291.5477 1290.5637 F 1556.7663 1648.8289 1631.8024 1630.8184 15

13 72.0808 1379.6478 1362.6212 1361.6372 1407.6427 1390.6161 1389.6321 V 1457.6979 1470.7183 1501.7605 1484.7340 1483.7499 14

14 72.0808 1478.7162 1461.6896 1460.7056 1506.7111 1489.6845 1488.7005 V 1358.6295 1371.6499 1402.6921 1385.6655 1384.6815 13

15 87.0553 1592.7591 1575.7326 1574.7485 1620.7540 1603.7275 1602.7435 N 1244.5866 1243.5913 1303.6237 1286.5971 1285.6131 12

16 86.0964 1705.8432 1688.8166 1687.8326 1733.8381 1716.8115 1715.8275 I 1131.5025 1144.5229 1158.5386 1189.5808 1172.5542 1171.5702 11

17 44.0495 1776.8803 1759.8537 1758.8697 1804.8752 1787.8486 1786.8646 A 1060.4654 1076.4967 1059.4701 1058.4861 10

18 101.0709 1904.9389 1887.9123 1886.9283 1932.9338 1915.9072 1914.9232 Q 932.4068 931.4116 1005.4596 988.4330 987.4490 9

19 86.0964 2018.0229 2000.9964 2000.0124 2046.0178 2028.9913 2028.0073 L 819.3227 818.3275 877.4010 860.3745 859.3904 8

20 60.0444 2105.0549 2088.0284 2087.0444 2133.0499 2116.0233 2115.0393 S 732.2907 731.2955 764.3169 747.2904 746.3064 7

21 101.0709 2233.1135 2216.0870 2215.1030 2261.1084 2244.0819 2243.0979 Q 604.2321 603.2369 677.2849 660.2584 659.2743 6

22 88.0393 2348.1405 2331.1139 2330.1299 2376.1354 2359.1088 2358.1248 D 489.2052 488.2100 549.2263 532.1998 531.2158 5

23 88.0393 2463.1674 2446.1409 2445.1568 2491.1623 2474.1358 2473.1518 D 374.1783 373.1830 434.1994 417.1728 416.1888 4

24 60.0444 2550.1994 2533.1729 2532.1889 2578.1944 2561.1678 2560.1838 S 287.1462 286.1510 319.1724 302.1459 301.1619 3

25 30.0338 2607.2209 2590.1944 2589.2103 2635.2158 2618.1893 2617.2053 G 232.1404 215.1139 2

26 129.1135 R 74.0237 73.0284 175.1190 158.0924 1

Seq ya yb Seq ya yb Seq ya yb

NL 201.1234 229.1183 NLT 302.1710 330.1660 NLTN 416.2140 444.2089

NLTNF 563.2824 591.2773 NLTNFP 660.3351 688.3301 LT 187.1441 215.1390

LTN 301.1870 329.1819 LTNF 448.2554 476.2504 LTNFP 545.3082 573.3031

LTNFPE 674.3508 702.3457 TN 188.1030 216.0979 TNF 335.1714 363.1663

TNFP 432.2241 460.2191 TNFPE 561.2667 589.2617 TNFPEN 676.2937 704.2886

NF 234.1237 262.1186 NFP 331.1765 359.1714 NFPE 460.2191 488.2140

NFPEN 575.2460 603.2409 NFPENG 632.2675 660.2624 FP 217.1335 245.1285

FPE 346.1761 374.1710 FPEN 461.2031 489.1980 FPENG 518.2245 546.2194

FPENGT 619.2722 647.2671 PE 199.1077 227.1026 PEN 314.1347 342.1296

PENG 371.1561 399.1510 PENGT 472.2038 500.1987 PENGTF 619.2722 647.2671

EN 217.0819 245.0768 ENG 274.1034 302.0983 ENGT 375.1510 403.1459

ENGTF 522.2194 550.2144 ENGTFV 621.2879 649.2828 NG 145.0608 173.0557

NGT 246.1084 274.1034 NGTF 393.1769 421.1718 NGTFV 492.2453 520.2402

NGTFVV 591.3137 619.3086 GT 131.0815 159.0764 GTF 278.1499 306.1448

GTFV 377.2183 405.2132 GTFVV 476.2867 504.2817 GTFVVN 590.3297 618.3246

TF 221.1285 249.1234 TFV 320.1969 348.1918 TFVV 419.2653 447.2602

TFVVN 533.3082 561.3031 TFVVNI 646.3923 674.3872 FV 219.1492 247.1441

FVV 318.2176 346.2125 FVVN 432.2605 460.2554 FVVNI 545.3446 573.3395

FVVNIA 616.3817 644.3766 VV 171.1492 199.1441 VVN 285.1921 313.1870

VVNI 398.2762 426.2711 VVNIA 469.3133 497.3082 VVNIAQ 597.3719 625.3668

VN 186.1237 214.1186 VNI 299.2078 327.2027 VNIA 370.2449 398.2398

VNIAQ 498.3035 526.2984 VNIAQL 611.3875 639.3824 VNIAQLS 698.4195 726.4145

NI 200.1394 228.1343 NIA 271.1765 299.1714 NIAQ 399.2350 427.2300

NIAQL 512.3191 540.3140 NIAQLS 599.3511 627.3461 IA 157.1335 185.1285

IAQ 285.1921 313.1870 IAQL 398.2762 426.2711 IAQLS 485.3082 513.3031

IAQLSQ 613.3668 641.3617 AQ 172.1081 200.1030 AQL 285.1921 313.1870

AQLS 372.2241 400.2191 AQLSQ 500.2827 528.2776 AQLSQD 615.3097 643.3046

QL 214.1550 242.1499 QLS 301.1870 329.1819 QLSQ 429.2456 457.2405

QLSQD 544.2726 572.2675 QLSQDD 659.2995 687.2944 LS 173.1285 201.1234

LSQ 301.1870 329.1819 LSQD 416.2140 444.2089 LSQDD 531.2409 559.2358

LSQDDS 618.2729 646.2679 LSQDDSG 675.2944 703.2893 SQ 188.1030 216.0979

SQD 303.1299 331.1248 SQDD 418.1569 446.1518 SQDDS 505.1889 533.1838

SQDDSG 562.2103 590.2053 QD 216.0979 244.0928 QDD 331.1248 359.1197

QDDS 418.1569 446.1518 QDDSG 475.1783 503.1732 DD 203.0662 231.0612

DDS 290.0983 318.0932 DDSG 347.1197 375.1147 DS 175.0713 203.0662

DSG 232.0928 260.0877 SG 117.0659 145.0608


1 129.0659 65.0366 112.0393 56.5233 Q 23

2 242.1499 121.5786 225.1234 113.0653 I 2398.2289 1199.6181 2381.2023 1191.1048 2380.2183 1190.6128 22

3 299.1714 150.0893 282.1448 141.5761 G 2285.1448 1143.0761 2268.1183 1134.5628 2267.1343 1134.0708 21

4 412.2554 206.6314 395.2289 198.1181 L 2228.1234 1114.5653 2211.0968 1106.0520 2210.1128 1105.5600 20

5 575.3188 288.1630 558.2922 279.6498 Y 2115.0393 1058.0233 2098.0128 1049.5100 2097.0287 1049.0180 19

6 672.3715 336.6894 655.3450 328.1761 P 1951.9760 976.4916 1934.9494 967.9784 1933.9654 967.4863 18

7 771.4400 386.2236 754.4134 377.7103 V 1854.9232 927.9652 1837.8967 919.4520 1836.9126 918.9600 17

8 884.5240 442.7656 867.4975 434.2524 L 1755.8548 878.4310 1738.8282 869.9178 1737.8442 869.4258 16

9 983.5924 492.2999 966.5659 483.7866 V 1642.7707 821.8890 1625.7442 813.3757 1624.7602 812.8837 15

10 1096.6765 548.8419 1079.6499 540.3286 I 1543.7023 772.3548 1526.6758 763.8415 1525.6918 763.3495 14

11 1211.7034 606.3554 1194.6769 597.8421 1193.6929 597.3501 D 1430.6183 715.8128 1413.5917 707.2995 1412.6077 706.8075 13

12 1298.7355 649.8714 1281.7089 641.3581 1280.7249 640.8661 S 1315.5913 658.2993 1298.5648 649.7860 1297.5807 649.2940 12

13 1385.7675 693.3874 1368.7409 684.8741 1367.7569 684.3821 S 1228.5593 614.7833 1211.5327 606.2700 1210.5487 605.7780 11

14 1442.7890 721.8981 1425.7624 713.3848 1424.7784 712.8928 G 1141.5273 571.2673 1124.5007 562.7540 1123.5167 562.2620 10

15 1605.8523 803.4298 1588.8257 794.9165 1587.8417 794.4245 Y 1084.5058 542.7565 1067.4792 534.2433 1066.4952 533.7513 9

16 1704.9207 852.9640 1687.8942 844.4507 1686.9101 843.9587 V 921.4425 461.2249 904.4159 452.7116 903.4319 452.2196 8

17 1818.9636 909.9855 1801.9371 901.4722 1800.9531 900.9802 N 822.3741 411.6907 805.3475 403.1774 804.3635 402.6854 7

18 1916.0164 958.5118 1898.9898 949.9986 1898.0058 949.5066 P 708.3311 354.6692 691.3046 346.1559 690.3206 345.6639 6

19 2031.0433 1016.0253 2014.0168 1007.5120 2013.0328 1007.0200 N 611.2784 306.1428 594.2518 297.6295 593.2678 297.1375 5

20 2194.1067 1097.5570 2177.0801 1089.0437 2176.0961 1088.5517 Y 496.2514 248.6293 479.2249 240.1161 478.2409 239.6241 4

21 2295.1543 1148.0808 2278.1278 1139.5675 2277.1438 1139.0755 T 333.1881 167.0977 316.1615 158.5844 315.1775 158.0924 3

22 2352.1758 1176.5915 2335.1493 1168.0783 2334.1652 1167.5863 G 232.1404 116.5738 215.1139 108.0606 2

23 R 175.1190 88.0631 158.0924 79.5498 1


1 187.0866 94.0469 W 18

2 302.1135 151.5604 285.0870 143.0471 N 1803.7814 902.3943 1786.7548 893.8811 1785.7708 893.3890 17

3 416.1565 208.5819 399.1299 200.0686 N 1688.7544 844.8809 1671.7279 836.3676 1670.7439 835.8756 16

4 517.2041 259.1057 500.1776 250.5924 499.1936 250.1004 T 1574.7115 787.8594 1557.6850 779.3461 1556.7009 778.8541 15

5 574.2256 287.6164 557.1990 279.1032 556.2150 278.6112 G 1473.6638 737.3356 1456.6373 728.8223 1455.6533 728.3303 14

6 734.2562 367.6318 717.2297 359.1185 716.2457 358.6265 C 1416.6424 708.8248 1399.6158 700.3115 1398.6318 699.8195 13

7 862.3148 431.6610 845.2883 423.1478 844.3043 422.6558 Q 1256.6117 628.8095 1239.5852 620.2962 1238.6012 619.8042 12

8 933.3519 467.1796 916.3254 458.6663 915.3414 458.1743 A 1128.5531 564.7802 1111.5266 556.2669 1110.5426 555.7749 11

9 1046.4360 523.7216 1029.4095 515.2084 1028.4254 514.7164 L 1057.5160 529.2617 1040.4895 520.7484 1039.5055 520.2564 10

10 1143.4888 572.2480 1126.4622 563.7347 1125.4782 563.2427 P 944.4320 472.7196 927.4054 464.2063 926.4214 463.7143 9

11 1230.5208 615.7640 1213.4942 607.2508 1212.5102 606.7588 S 847.3792 424.1932 830.3527 415.6800 829.3686 415.1880 8

12 1358.5794 679.7933 1341.5528 671.2800 1340.5688 670.7880 Q 760.3472 380.6772 743.3206 372.1640 742.3366 371.6719 7

13 1473.6063 737.3068 1456.5798 728.7935 1455.5957 728.3015 D 632.2886 316.6479 615.2620 308.1347 614.2780 307.6427 6

14 1602.6489 801.8281 1585.6224 793.3148 1584.6383 792.8228 E 517.2617 259.1345 500.2351 250.6212 499.2511 250.1292 5

15 1659.6704 830.3388 1642.6438 821.8255 1641.6598 821.3335 G 388.2191 194.6132 371.1925 186.0999 370.2085 185.6079 4

16 1756.7231 878.8652 1739.6966 870.3519 1738.7126 869.8599 P 331.1976 166.1024 314.1710 157.5892 313.1870 157.0972 3

17 1843.7552 922.3812 1826.7286 913.8679 1825.7446 913.3759 S 234.1448 117.5761 217.1183 109.0628 216.1343 108.5708 2

18 K 147.1128 74.0600 130.0863 65.5468 1

b b++ b* b*++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #

1 114.0913 57.5493 L 22

2 201.1234 101.0653 183.1128 92.0600 S 2290.1925 1145.5999 2273.1660 1137.0866 2272.1819 1136.5946 21

3 314.2074 157.6074 296.1969 148.6021 L 2203.1605 1102.0839 2186.1339 1093.5706 2185.1499 1093.0786 20

4 427.2915 214.1494 409.2809 205.1441 L 2090.0764 1045.5418 2073.0499 1037.0286 2072.0659 1036.5366 19

5 556.3341 278.6707 538.3235 269.6654 E 1976.9924 988.9998 1959.9658 980.4865 1958.9818 979.9945 18

6 685.3767 343.1920 667.3661 334.1867 E 1847.9498 924.4785 1830.9232 915.9652 1829.9392 915.4732 17

7 782.4294 391.7184 764.4189 382.7131 P 1718.9072 859.9572 1701.8806 851.4439 1700.8966 850.9519 16

8 839.4509 420.2291 821.4403 411.2238 G 1621.8544 811.4308 1604.8279 802.9176 1603.8438 802.4256 15

9 954.4778 477.7426 937.4513 469.2293 936.4673 468.7373 N 1564.8329 782.9201 1547.8064 774.4068 1546.8224 773.9148 14

10 1011.4993 506.2533 994.4728 497.7400 993.4887 497.2480 G 1449.8060 725.4066 1432.7795 716.8934 1431.7954 716.4014 13

11 1112.5470 556.7771 1095.5204 548.2639 1094.5364 547.7719 T 1392.7845 696.8959 1375.7580 688.3826 1374.7740 687.8906 12

12 1259.6154 630.3113 1242.5889 621.7981 1241.6048 621.3061 F 1291.7369 646.3721 1274.7103 637.8588 1273.7263 637.3668 11

13 1360.6631 680.8352 1343.6365 672.3219 1342.6525 671.8299 T 1144.6684 572.8379 1127.6419 564.3246 1126.6579 563.8326 10

14 1459.7315 730.3694 1442.7049 721.8561 1441.7209 721.3641 V 1043.6208 522.3140 1026.5942 513.8007 1025.6102 513.3087 9

15 1572.8156 786.9114 1555.7890 778.3981 1554.8050 777.9061 I 944.5524 472.7798 927.5258 464.2665 926.5418 463.7745 8

16 1685.8996 843.4535 1668.8731 834.9402 1667.8891 834.4482 L 831.4683 416.2378 814.4417 407.7245 813.4577 407.2325 7

17 1799.9426 900.4749 1782.9160 891.9616 1781.9320 891.4696 N 718.3842 359.6958 701.3577 351.1825 700.3737 350.6905 6

18 1928.0011 964.5042 1910.9746 955.9909 1909.9906 955.4989 Q 604.3413 302.6743 587.3148 294.1610 586.3307 293.6690 5

19 2041.0852 1021.0462 2024.0586 1012.5330 2023.0746 1012.0410 L 476.2827 238.6450 459.2562 230.1317 458.2722 229.6397 4

20 2142.1329 1071.5701 2125.1063 1063.0568 2124.1223 1062.5648 T 363.1987 182.1030 346.1721 173.5897 345.1881 173.0977 3

21 2229.1649 1115.0861 2212.1384 1106.5728 2211.1543 1106.0808 S 262.1510 131.5791 245.1244 123.0659 244.1404 122.5738 2

22 R 175.1190 88.0631 158.0924 79.5498 1

From MALDI-TOF/TOF


1 58.0287 29.5180 G 21

2 171.1128 86.0600 L 2120.0757 1060.5415 2103.0492 1052.0282 2102.0652 1051.5362 20

3 258.1448 129.5761 240.1343 120.5708 S 2006.9917 1003.9995 1989.9651 995.4862 1988.9811 994.9942 19

4 405.2132 203.1103 387.2027 194.1050 F 1919.9597 960.4835 1902.9331 951.9702 1901.9491 951.4782 18

5 520.2402 260.6237 502.2296 251.6184 D 1772.8912 886.9493 1755.8647 878.4360 1754.8807 877.9440 17

6 619.3086 310.1579 601.2980 301.1527 V 1657.8643 829.4358 1640.8378 820.9225 1639.8537 820.4305 16

7 706.3406 353.6740 688.3301 344.6687 S 1558.7959 779.9016 1541.7693 771.3883 1540.7853 770.8963 15

8 819.4247 410.2160 801.4141 401.2107 L 1471.7639 736.3856 1454.7373 727.8723 1453.7533 727.3803 14

9 948.4673 474.7373 930.4567 465.7320 E 1358.6798 679.8435 1341.6532 671.3303 1340.6692 670.8383 13

10 1047.5357 524.2715 1029.5251 515.2662 V 1229.6372 615.3222 1212.6107 606.8090 1211.6266 606.3170 12

11 1134.5677 567.7875 1116.5572 558.7822 S 1130.5688 565.7880 1113.5422 557.2748 1112.5582 556.7827 11

12 1262.6263 631.8168 1245.5998 623.3035 1244.6157 622.8115 Q 1043.5368 522.2720 1026.5102 513.7587 1025.5262 513.2667 10

13 1319.6478 660.3275 1302.6212 651.8142 1301.6372 651.3222 G 915.4782 458.2427 898.4516 449.7295 897.4676 449.2374 9

14 1416.7005 708.8539 1399.6740 700.3406 1398.6900 699.8486 P 858.4567 429.7320 841.4302 421.2187 840.4462 420.7267 8

15 1473.7220 737.3646 1456.6955 728.8514 1455.7114 728.3594 G 761.4040 381.2056 744.3774 372.6923 743.3934 372.2003 7

16 1586.8061 793.9067 1569.7795 785.3934 1568.7955 784.9014 L 704.3825 352.6949 687.3559 344.1816 686.3719 343.6896 6

17 1699.8901 850.4487 1682.8636 841.9354 1681.8796 841.4434 L 591.2984 296.1529 574.2719 287.6396 573.2879 287.1476 5

18 1814.9171 907.9622 1797.8905 899.4489 1796.9065 898.9569 N 478.2144 239.6108 461.1878 231.0975 460.2038 230.6055 4

19 1929.9440 965.4756 1912.9175 956.9624 1911.9334 956.4704 D 363.1874 182.0974 346.1609 173.5841 345.1769 173.0921 3

20 2030.9917 1015.9995 2013.9651 1007.4862 2012.9811 1006.9942 T 248.1605 124.5839 231.1339 116.0706 230.1499 115.5786 2

21 K 147.1128 74.0600 130.0863 65.5468 1


1 100.0757 50.5415 V 22

2 197.1285 99.0679 P 2325.1730 1163.0901 2308.1464 1154.5769 2307.1624 1154.0848 21

3 254.1499 127.5786 G 2228.1202 1114.5637 2211.0937 1106.0505 2210.1097 1105.5585 20

4 369.1769 185.0921 352.1503 176.5788 N 2171.0988 1086.0530 2154.0722 1077.5397 2153.0882 1077.0477 19

5 468.2453 234.6263 451.2187 226.1130 V 2056.0718 1028.5395 2039.0453 1020.0263 2038.0613 1019.5343 18

6 569.2929 285.1501 552.2664 276.6368 551.2824 276.1448 T 1957.0034 979.0053 1939.9769 970.4921 1938.9928 970.0001 17

7 640.3301 320.6687 623.3035 312.1554 622.3195 311.6634 A 1855.9557 928.4815 1838.9292 919.9682 1837.9452 919.4762 16

8 739.3985 370.2029 722.3719 361.6896 721.3879 361.1976 V 1784.9186 892.9629 1767.8921 884.4497 1766.9080 883.9577 15

9 852.4825 426.7449 835.4560 418.2316 834.4720 417.7396 L 1685.8502 843.4287 1668.8236 834.9155 1667.8396 834.4235 14

10 909.5040 455.2556 892.4775 446.7424 891.4934 446.2504 G 1572.7661 786.8867 1555.7396 778.3734 1554.7556 777.8814 13

11 1038.5466 519.7769 1021.5200 511.2637 1020.5360 510.7717 E 1515.7447 758.3760 1498.7181 749.8627 1497.7341 749.3707 12

12 1139.5943 570.3008 1122.5677 561.7875 1121.5837 561.2955 T 1386.7021 693.8547 1369.6755 685.3414 1368.6915 684.8494 11

13 1252.6783 626.8428 1235.6518 618.3295 1234.6678 617.8375 L 1285.6544 643.3308 1268.6278 634.8176 10

14 1380.7733 690.8903 1363.7468 682.3770 1362.7627 681.8850 K 1172.5703 586.7888 1155.5438 578.2755 9

15 1479.8417 740.4245 1462.8152 731.9112 1461.8312 731.4192 V 1044.4754 522.7413 1027.4488 514.2280 8

16 1576.8945 788.9509 1559.8679 780.4376 1558.8839 779.9456 P 945.4070 473.2071 928.3804 464.6938 7

17 1736.9251 868.9662 1719.8986 860.4529 1718.9146 859.9609 C 848.3542 424.6807 831.3276 416.1675 6

18 1873.9840 937.4957 1856.9575 928.9824 1855.9735 928.4904 H 688.3235 344.6654 671.2970 336.1521 5

19 2021.0525 1011.0299 2004.0259 1002.5166 2003.0419 1002.0246 F 551.2646 276.1360 534.2381 267.6227 4

20 2118.1052 1059.5562 2101.0787 1051.0430 2100.0947 1050.5510 P 404.1962 202.6017 387.1697 194.0885 3

21 2278.1359 1139.5716 2261.1093 1131.0583 2260.1253 1130.5663 C 307.1435 154.0754 290.1169 145.5621 2

22 K 147.1128 74.0600 130.0863 65.5468 1

IG α-2 HEAVY CHAIN


1 102.0550 51.5311 84.0444 42.5258 T 9

2 199.1077 100.0575 181.0972 91.0522 P 858.4931 429.7502 841.4666 421.2369 840.4825 420.7449 8

3 312.1918 156.5995 294.1812 147.5942 L 761.4403 381.2238 744.4138 372.7105 743.4298 372.2185 7

4 413.2395 207.1234 395.2289 198.1181 T 648.3563 324.6818 631.3297 316.1685 630.3457 315.6765 6

5 484.2766 242.6419 466.2660 233.6366 A 547.3086 274.1579 530.2820 265.6447 529.2980 265.1527 5

6 599.3035 300.1554 582.2770 291.6421 581.2929 291.1501 N 476.2715 238.6394 459.2449 230.1261 458.2609 229.6341 4

7 712.3876 356.6974 695.3610 348.1842 694.3770 347.6921 I 361.2445 181.1259 344.2180 172.6126 343.2340 172.1206 3

8 813.4353 407.2213 796.4087 398.7080 795.4247 398.2160 T 248.1605 124.5839 231.1339 116.0706 230.1499 115.5786 2

9 K 147.1128 74.0600 130.0863 65.5468 1

Monoisotopic mass of neutral peptide Mr(calc): 2963.5750 Fixed modifications: Carbamidomethyl (C) Variable modifications: N18 : Deamidated (NQ) Ions Score: 32 Expect: 0.11 Matches (Bold Red): 30/543 fragment ions using 78 most intense peaks # Immon. a a* a0 b b* b0 d d' Seq. v w w' y y* y0 #

1 86.0964 86.0964 114.0913 L 27

2 60.0444 173.1285 155.1179 201.1234 183.1128 S 2819.4720 2818.4768 2851.4982 2834.4717 2833.4876 26

3 86.0964 286.2125 268.2020 314.2074 296.1969 L 2706.3879 2705.3927 2764.4662 2747.4396 2746.4556 25

4 110.0713 423.2714 405.2609 451.2663 433.2558 H 2569.3290 2651.3821 2634.3556 2633.3716 24

5 129.1135 579.3725 562.3460 561.3620 607.3675 590.3409 589.3569 494.3085 R 2413.2279 2412.2327 2514.3232 2497.2967 2496.3126 23

6 70.0651 676.4253 659.3988 658.4147 704.4202 687.3937 686.4097 650.4097 P 2316.1751 2315.1799 2358.2221 2341.1955 2340.2115 22

7 44.0495 747.4624 730.4359 729.4519 775.4573 758.4308 757.4468 A 2245.1380 2261.1693 2244.1428 2243.1588 21

8 86.0964 860.5465 843.5199 842.5359 888.5414 871.5148 870.5308 818.4995 L 2132.0540 2131.0587 2190.1322 2173.1057 2172.1217 20

9 102.0550 989.5891 972.5625 971.5785 1017.5840 1000.5574 999.5734 931.5836 E 2003.0114 2002.0161 2077.0482 2060.0216 2059.0376 19

10 88.0393 1104.6160 1087.5895 1086.6055 1132.6109 1115.5844 1114.6004 1060.6262 D 1887.9844 1886.9892 1948.0056 1930.9790 1929.9950 18

11 86.0964 1217.7001 1200.6735 1199.6895 1245.6950 1228.6684 1227.6844 1175.6531 L 1774.9004 1773.9051 1832.9786 1815.9521 1814.9681 17

12 86.0964 1330.7841 1313.7576 1312.7736 1358.7791 1341.7525 1340.7685 1288.7372 L 1661.8163 1660.8211 1719.8946 1702.8680 1701.8840 16

13 86.0964 1443.8682 1426.8417 1425.8576 1471.8631 1454.8366 1453.8526 1401.8213 L 1548.7322 1547.7370 1606.8105 1589.7839 1588.7999 15

14 30.0338 1500.8897 1483.8631 1482.8791 1528.8846 1511.8580 1510.8740 G 1493.7264 1476.6999 1475.7159 14

15 60.0444 1587.9217 1570.8952 1569.9111 1615.9166 1598.8901 1597.9061 1571.9268 S 1404.6788 1403.6835 1436.7050 1419.6784 1418.6944 13

16 102.0550 1716.9643 1699.9377 1698.9537 1744.9592 1727.9327 1726.9486 1658.9588 E 1275.6362 1274.6409 1349.6729 1332.6464 1331.6624 12

17 44.0495 1788.0014 1770.9749 1769.9908 1815.9963 1798.9698 1797.9858 A 1204.5990 1220.6303 1203.6038 1202.6198 11

18 88.0393 1903.0283 1886.0018 1885.0178 1931.0233 1913.9967 1913.0127 1859.0385 N 1089.5721 1088.5769 1149.5932 1132.5667 1131.5827 10

19 86.0964 2016.1124 1999.0859 1998.1018 2044.1073 2027.0808 2026.0968 1974.0655 L 976.4880 975.4928 1034.5663 1017.5397 1016.5557 9

20 74.0600 2117.1601 2100.1335 2099.1495 2145.1550 2128.1285 2127.1444 2101.1652 2103.1444 T 875.4404 888.4608 890.4400 921.4822 904.4557 903.4717 8

21 133.0430 2277.1907 2260.1642 2259.1802 2305.1857 2288.1591 2287.1751 2188.1972 C 715.4097 714.4145 820.4346 803.4080 802.4240 7

22 74.0600 2378.2384 2361.2119 2360.2279 2406.2333 2389.2068 2388.2228 2362.2435 2364.2228 T 614.3620 627.3824 629.3617 660.4039 643.3774 642.3933 6

23 86.0964 2491.3225 2474.2959 2473.3119 2519.3174 2502.2908 2501.3068 2449.2755 L 501.2780 500.2827 559.3562 542.3297 541.3457 5

24 74.0600 2592.3702 2575.3436 2574.3596 2620.3651 2603.3385 2602.3545 2576.3752 2578.3545 T 400.2303 413.2507 415.2300 446.2722 429.2456 428.2616 4

25 30.0338 2649.3916 2632.3651 2631.3811 2677.3865 2660.3600 2659.3760 G 345.2245 328.1979 3

26 86.0964 2762.4757 2745.4491 2744.4651 2790.4706 2773.4441 2772.4600 2720.4287 L 230.1248 229.1295 288.2030 271.1765 2

27 129.1135 R 74.0237 73.0284 175.1190 158.0924 1


SL 173.1285 201.1234 SLH 310.1874 338.1823 SLHR 466.2885 494.2834

SLHRP 563.3412 591.3362 SLHRPA 634.3784 662.3733 LH 223.1553 251.1503

LHR 379.2564 407.2514 LHRP 476.3092 504.3041 LHRPA 547.3463 575.3412

LHRPAL 660.4304 688.4253 HR 266.1724 294.1673 HRP 363.2251 391.2201

HRPA 434.2623 462.2572 HRPAL 547.3463 575.3412 HRPALE 676.3889 704.3838

RP 226.1662 254.1612 RPA 297.2034 325.1983 RPAL 410.2874 438.2823

RPALE 539.3300 567.3249 RPALED 654.3570 682.3519 PA 141.1022 169.0972

PAL 254.1863 282.1812 PALE 383.2289 411.2238 PALED 498.2558 526.2508

PALEDL 611.3399 639.3348 AL 157.1335 185.1285 ALE 286.1761 314.1710

ALED 401.2031 429.1980 ALEDL 514.2871 542.2821 ALEDLL 627.3712 655.3661

LE 215.1390 243.1339 LED 330.1660 358.1609 LEDL 443.2500 471.2449

LEDLL 556.3341 584.3290 LEDLLL 669.4182 697.4131 ED 217.0819 245.0768

EDL 330.1660 358.1609 EDLL 443.2500 471.2449 EDLLL 556.3341 584.3290

EDLLLG 613.3556 641.3505 DL 201.1234 229.1183 DLL 314.2074 342.2023

DLLL 427.2915 455.2864 DLLLG 484.3130 512.3079 DLLLGS 571.3450 599.3399

LL 199.1805 227.1754 LLL 312.2646 340.2595 LLLG 369.2860 397.2809

LLLGS 456.3180 484.3130 LLLGSE 585.3606 613.3556 LLLGSEA 656.3978 684.3927

LL 199.1805 227.1754 LLG 256.2020 284.1969 LLGS 343.2340 371.2289

LLGSE 472.2766 500.2715 LLGSEA 543.3137 571.3086 LLGSEAN 658.3406 686.3355

LG 143.1179 171.1128 LGS 230.1499 258.1448 LGSE 359.1925 387.1874

LGSEA 430.2296 458.2245 LGSEAN 545.2566 573.2515 LGSEANL 658.3406 686.3355

GS 117.0659 145.0608 GSE 246.1084 274.1034 GSEA 317.1456 345.1405

GSEAN 432.1725 460.1674 GSEANL 545.2566 573.2515 GSEANLT 646.3042 674.2992

SE 189.0870 217.0819 SEA 260.1241 288.1190 SEAN 375.1510 403.1459

SEANL 488.2351 516.2300 SEANLT 589.2828 617.2777 EA 173.0921 201.0870

EAN 288.1190 316.1139 EANL 401.2031 429.1980 EANLT 502.2507 530.2457

EANLTC 662.2814 690.2763 AN 159.0764 187.0713 ANL 272.1605 300.1554

ANLT 373.2082 401.2031 ANLTC 533.2388 561.2337 ANLTCT 634.2865 662.2814

NL 201.1234 229.1183 NLT 302.1710 330.1660 NLTC 462.2017 490.1966

NLTCT 563.2494 591.2443 NLTCTL 676.3334 704.3283 LT 187.1441 215.1390

LTC 347.1748 375.1697 LTCT 448.2224 476.2173 LTCTL 561.3065 589.3014

LTCTLT 662.3542 690.3491 TC 234.0907 262.0856 TCT 335.1384 363.1333

TCTL 448.2224 476.2173 TCTLT 549.2701 577.2650 TCTLTG 606.2916 634.2865

CT 234.0907 262.0856 CTL 347.1748 375.1697 CTLT 448.2224 476.2173

CTLTG 505.2439 533.2388 CTLTGL 618.3280 646.3229 TL 187.1441 215.1390

TLT 288.1918 316.1867 TLTG 345.2132 373.2082 TLTGL 458.2973 486.2922

LT 187.1441 215.1390 LTG 244.1656 272.1605 LTGL 357.2496 385.2445

TG 131.0815 159.0764 TGL 244.1656 272.1605 GL 143.1179 171.1128

Manual assignment of the spectrum MALDI-TOF/TOF (expanded view along the Y axis) to the peptide ion 2964.55 to LSLHRPALEDLLLGSEAN231LTCTLTGLR of Ig a-2chain deamidated at the N231 site.


1 100.0757 50.5415 V 44

2 247.1441 124.0757 F 4678.2770 2339.6421 4661.2504 2331.1289 4660.2664 2330.6369 43

3 344.1969 172.6021 P 4531.2086 2266.1079 4514.1820 2257.5947 4513.1980 2257.1026 42

4 457.2809 229.1441 L 4434.1558 2217.5815 4417.1293 2209.0683 4416.1453 2208.5763 41

5 544.3130 272.6601 526.3024 263.6548 S 4321.0718 2161.0395 4304.0452 2152.5262 4303.0612 2152.0342 40

6 657.3970 329.2022 639.3865 320.1969 L 4234.0397 2117.5235 4217.0132 2109.0102 4216.0292 2108.5182 39

7 772.4240 386.7156 754.4134 377.7103 D 4120.9557 2060.9815 4103.9291 2052.4682 4102.9451 2051.9762 38

8 859.4560 430.2316 841.4454 421.2264 S 4005.9287 2003.4680 3988.9022 1994.9547 3987.9182 1994.4627 37

9 960.5037 480.7555 942.4931 471.7502 T 3918.8967 1959.9520 3901.8701 1951.4387 3900.8861 1950.9467 36

10 1057.5564 529.2819 1039.5459 520.2766 P 3817.8490 1909.4281 3800.8225 1900.9149 3799.8384 1900.4229 35

11 1185.6150 593.3111 1168.5885 584.7979 1167.6045 584.3059 Q 3720.7962 1860.9018 3703.7697 1852.3885 3702.7857 1851.8965 34

12 1300.6420 650.8246 1283.6154 642.3113 1282.6314 641.8193 D 3592.7377 1796.8725 3575.7111 1788.3592 3574.7271 1787.8672 33

13 1357.6634 679.3353 1340.6369 670.8221 1339.6529 670.3301 G 3477.7107 1739.3590 3460.6842 1730.8457 3459.7002 1730.3537 32

14 1471.7063 736.3568 1454.6798 727.8435 1453.6958 727.3515 N 3420.6893 1710.8483 3403.6627 1702.3350 3402.6787 1701.8430 31

15 1570.7748 785.8910 1553.7482 777.3777 1552.7642 776.8857 V 3306.6463 1653.8268 3289.6198 1645.3135 3288.6358 1644.8215 30

16 1669.8432 835.4252 1652.8166 826.9120 1651.8326 826.4199 V 3207.5779 1604.2926 3190.5514 1595.7793 3189.5674 1595.2873 29

17 1768.9116 884.9594 1751.8850 876.4462 1750.9010 875.9542 V 3108.5095 1554.7584 3091.4830 1546.2451 3090.4989 1545.7531 28

18 1839.9487 920.4780 1822.9222 911.9647 1821.9381 911.4727 A 3009.4411 1505.2242 2992.4145 1496.7109 2991.4305 1496.2189 27

19 1999.9794 1000.4933 1982.9528 991.9800 1981.9688 991.4880 C 2938.4040 1469.7056 2921.3774 1461.1924 2920.3934 1460.7003 26

20 2113.0634 1057.0353 2096.0369 1048.5221 2095.0529 1048.0301 L 2778.3733 1389.6903 2761.3468 1381.1770 2760.3628 1380.6850 25

21 2212.1318 1106.5696 2195.1053 1098.0563 2194.1213 1097.5643 V 2665.2893 1333.1483 2648.2627 1324.6350 2647.2787 1324.1430 24

22 2340.1904 1170.5988 2323.1639 1162.0856 2322.1798 1161.5936 Q 2566.2209 1283.6141 2549.1943 1275.1008 2548.2103 1274.6088 23

23 2397.2119 1199.1096 2380.1853 1190.5963 2379.2013 1190.1043 G 2438.1623 1219.5848 2421.1357 1211.0715 2420.1517 1210.5795 22

24 2544.2803 1272.6438 2527.2537 1264.1305 2526.2697 1263.6385 F 2381.1408 1191.0740 2364.1143 1182.5608 2363.1302 1182.0688 21

25 2691.3487 1346.1780 2674.3222 1337.6647 2673.3381 1337.1727 F 2234.0724 1117.5398 2217.0458 1109.0266 2216.0618 1108.5346 20

26 2788.4015 1394.7044 2771.3749 1386.1911 2770.3909 1385.6991 P 2087.0040 1044.0056 2069.9774 1035.4924 2068.9934 1035.0003 19

27 2916.4600 1458.7337 2899.4335 1450.2204 2898.4495 1449.7284 Q 1989.9512 995.4792 1972.9247 986.9660 1971.9407 986.4740 18

28 3045.5026 1523.2550 3028.4761 1514.7417 3027.4921 1514.2497 E 1861.8926 931.4500 1844.8661 922.9367 1843.8821 922.4447 17

29 3142.5554 1571.7813 3125.5289 1563.2681 3124.5448 1562.7761 P 1732.8500 866.9287 1715.8235 858.4154 1714.8395 857.9234 16

30 3255.6395 1628.3234 3238.6129 1619.8101 3237.6289 1619.3181 L 1635.7973 818.4023 1618.7707 809.8890 1617.7867 809.3970 15

31 3342.6715 1671.8394 3325.6449 1663.3261 3324.6609 1662.8341 S 1522.7132 761.8602 1505.6867 753.3470 1504.7027 752.8550 14

32 3441.7399 1721.3736 3424.7134 1712.8603 3423.7293 1712.3683 V 1435.6812 718.3442 1418.6546 709.8310 1417.6706 709.3390 13

33 3542.7876 1771.8974 3525.7610 1763.3842 3524.7770 1762.8921 T 1336.6128 668.8100 1319.5862 660.2968 1318.6022 659.8047 12

34 3728.8669 1864.9371 3711.8404 1856.4238 3710.8563 1855.9318 W 1235.5651 618.2862 1218.5386 609.7729 1217.5545 609.2809 11

35 3815.8989 1908.4531 3798.8724 1899.9398 3797.8884 1899.4478 S 1049.4858 525.2465 1032.4592 516.7333 1031.4752 516.2412 10

36 3944.9415 1972.9744 3927.9150 1964.4611 3926.9310 1963.9691 E 962.4538 481.7305 945.4272 473.2172 944.4432 472.7252 9

37 4031.9735 2016.4904 4014.9470 2007.9771 4013.9630 2007.4851 S 833.4112 417.2092 816.3846 408.6959 815.4006 408.2039 8

38 4088.9950 2045.0011 4071.9685 2036.4879 4070.9844 2035.9959 G 746.3791 373.6932 729.3526 365.1799 728.3686 364.6879 7

39 4217.0536 2109.0304 4200.0270 2100.5172 4199.0430 2100.0252 Q 689.3577 345.1825 672.3311 336.6692 671.3471 336.1772 6

40 4332.0805 2166.5439 4315.0540 2158.0306 4314.0700 2157.5386 N 561.2991 281.1532 544.2725 272.6399 543.2885 272.1479 5

41 4431.1489 2216.0781 4414.1224 2207.5648 4413.1384 2207.0728 V 446.2722 223.6397 429.2456 215.1264 428.2616 214.6344 4

42 4532.1966 2266.6019 4515.1701 2258.0887 4514.1861 2257.5967 T 347.2037 174.1055 330.1772 165.5922 329.1932 165.1002 3

43 4603.2337 2302.1205 4586.2072 2293.6072 4585.2232 2293.1152 A 246.1561 123.5817 229.1295 115.0684 2

44 R 175.1190 88.0631 158.0924 79.5498 1

COMPLEMENT COMPONENT C4A (P01028)


1 58.0287 29.5180 G 11

2 171.1128 86.0600 L 1048.5633 524.7853 1031.5368 516.2720 1030.5527 515.7800 10

3 286.1397 143.5735 269.1132 135.0602 N 935.4792 468.2433 918.4527 459.7300 917.4687 459.2380 9

4 385.2082 193.1077 368.1816 184.5944 V 820.4523 410.7298 803.4258 402.2165 802.4417 401.7245 8

5 486.2558 243.6316 469.2293 235.1183 468.2453 234.6263 T 721.3839 361.1956 704.3573 352.6823 703.3733 352.1903 7

6 599.3399 300.1736 582.3133 291.6603 581.3293 291.1683 L 620.3362 310.6717 603.3097 302.1585 602.3256 301.6665 6

7 686.3719 343.6896 669.3454 335.1763 668.3614 334.6843 S 507.2522 254.1297 490.2256 245.6164 489.2416 245.1244 5

8 773.4040 387.2056 756.3774 378.6923 755.3934 378.2003 S 420.2201 210.6137 403.1936 202.1004 402.2096 201.6084 4

9 874.4516 437.7295 857.4251 429.2162 856.4411 428.7242 T 333.1881 167.0977 316.1615 158.5844 315.1775 158.0924 3

10 931.4731 466.2402 914.4465 457.7269 913.4625 457.2349 G 232.1404 116.5738 215.1139 108.0606 2

11 R 175.1190 88.0631 158.0924 79.5498 1

LACTADHERIN ( Q08431)


1 116.0502 58.0287 98.0237 49.5155 N 16

2 230.0771 115.5422 213.0506 107.0289 N 1639.8174 820.4123 1622.7908 811.8990 1621.8068 811.4070 15

3 317.1092 159.0582 300.0826 150.5449 299.0986 150.0529 S 1524.7904 762.8988 1507.7639 754.3856 1506.7798 753.8936 14

4 430.1932 215.6003 413.1667 207.0870 412.1827 206.5950 I 1437.7584 719.3828 1420.7318 710.8696 1419.7478 710.3775 13

5 527.2460 264.1266 510.2194 255.6134 509.2354 255.1214 P 1324.6743 662.8408 1307.6478 654.3275 1306.6638 653.8355 12

6 642.2729 321.6401 625.2464 313.1268 624.2624 312.6348 D 1227.6216 614.3144 1210.5950 605.8011 1209.6110 605.3091 11

7 770.3679 385.6876 753.3414 377.1743 752.3573 376.6823 K 1112.5946 556.8009 1095.5681 548.2877 1094.5841 547.7957 10

8 898.4265 449.7169 881.3999 441.2036 880.4159 440.7116 Q 984.4997 492.7535 967.4731 484.2402 966.4891 483.7482 9

9 1011.5105 506.2589 994.4840 497.7456 993.5000 497.2536 I 856.4411 428.7242 839.4145 420.2109 838.4305 419.7189 8

10 1112.5582 556.7827 1095.5317 548.2695 1094.5477 547.7775 T 743.3570 372.1821 726.3305 363.6689 725.3464 363.1769 7

11 1183.5953 592.3013 1166.5688 583.7880 1165.5848 583.2960 A 642.3093 321.6583 625.2828 313.1450 624.2988 312.6530 6

12 1270.6274 635.8173 1253.6008 627.3040 1252.6168 626.8120 S 571.2722 286.1397 554.2457 277.6265 553.2617 277.1345 5

13 1357.6594 679.3333 1340.6328 670.8201 1339.6488 670.3281 S 484.2402 242.6237 467.2136 234.1105 466.2296 233.6185 4

14 1444.6914 722.8493 1427.6649 714.3361 1426.6809 713.8441 S 397.2082 199.1077 380.1816 190.5944 379.1976 190.1024 3

15 1607.7547 804.3810 1590.7282 795.8677 1589.7442 795.3757 Y 310.1761 155.5917 293.1496 147.0784 2

16 K 147.1128 74.0600 130.0863 65.5468 1

BUTYROPHILIN SUBFAMILY 1 MEMBER (Q13410)

TENASCIN-C (P24821)


1 114.0913 57.5493 L 27

2 229.1183 115.0628 212.0917 106.5495 N 3004.4435 1502.7254 2987.4170 1494.2121 2986.4330 1493.7201 26

3 415.1976 208.1024 398.1710 199.5892 W 2889.4166 1445.2119 2872.3900 1436.6987 2871.4060 1436.2067 25

4 516.2453 258.6263 499.2187 250.1130 498.2347 249.6210 T 2703.3373 1352.1723 2686.3107 1343.6590 2685.3267 1343.1670 24

5 587.2824 294.1448 570.2558 285.6316 569.2718 285.1395 A 2602.2896 1301.6484 2585.2631 1293.1352 2584.2790 1292.6432 23

6 658.3195 329.6634 641.2929 321.1501 640.3089 320.6581 A 2531.2525 1266.1299 2514.2259 1257.6166 2513.2419 1257.1246 22

7 773.3464 387.1769 756.3199 378.6636 755.3359 378.1716 D 2460.2154 1230.6113 2443.1888 1222.0981 2442.2048 1221.6060 21

8 902.3890 451.6982 885.3625 443.1849 884.3785 442.6929 Q 2345.1884 1173.0979 2328.1619 1164.5846 2327.1779 1164.0926 20

9 973.4261 487.2167 956.3996 478.7034 955.4156 478.2114 A 2216.1458 1108.5766 2199.1193 1100.0633 2198.1353 1099.5713 19

10 1136.4895 568.7484 1119.4629 560.2351 1118.4789 559.7431 Y 2145.1087 1073.0580 2128.0822 1064.5447 2127.0982 1064.0527 18

11 1265.5321 633.2697 1248.5055 624.7564 1247.5215 624.2644 E 1982.0454 991.5263 1965.0189 983.0131 1964.0348 982.5211 17

12 1402.5910 701.7991 1385.5644 693.2859 1384.5804 692.7938 H 1853.0028 927.0050 1835.9763 918.4918 1834.9922 917.9998 16

13 1549.6594 775.3333 1532.6328 766.8201 1531.6488 766.3280 F 1715.9439 858.4756 1698.9173 849.9623 1697.9333 849.4703 15

14 1662.7435 831.8754 1645.7169 823.3621 1644.7329 822.8701 I 1568.8755 784.9414 1551.8489 776.4281 1550.8649 775.9361 14

15 1775.8275 888.4174 1758.8010 879.9041 1757.8170 879.4121 I 1455.7914 728.3993 1438.7649 719.8861 1437.7809 719.3941 13

16 1903.8861 952.4467 1886.8595 943.9334 1885.8755 943.4414 Q 1342.7074 671.8573 1325.6808 663.3440 1324.6968 662.8520 12

17 2002.9545 1001.9809 1985.9280 993.4676 1984.9439 992.9756 V 1214.6488 607.8280 1197.6222 599.3148 1196.6382 598.8227 11

18 2131.0131 1066.0102 2113.9865 1057.4969 2113.0025 1057.0049 Q 1115.5804 558.2938 1098.5538 549.7805 1097.5698 549.2885 10

19 2260.0557 1130.5315 2243.0291 1122.0182 2242.0451 1121.5262 E 987.5218 494.2645 970.4952 485.7513 969.5112 485.2592 9

20 2331.0928 1166.0500 2314.0662 1157.5368 2313.0822 1157.0448 A 858.4792 429.7432 841.4526 421.2300 840.4686 420.7380 8

21 2445.1357 1223.0715 2428.1092 1214.5582 2427.1252 1214.0662 N 787.4421 394.2247 770.4155 385.7114 769.4315 385.2194 7

22 2573.2307 1287.1190 2556.2041 1278.6057 2555.2201 1278.1137 K 673.3992 337.2032 656.3726 328.6899 655.3886 328.1979 6

23 2672.2991 1336.6532 2655.2725 1328.1399 2654.2885 1327.6479 V 545.3042 273.1557 528.2776 264.6425 527.2936 264.1504 5

24 2801.3417 1401.1745 2784.3151 1392.6612 2783.3311 1392.1692 E 446.2358 223.6215 429.2092 215.1083 428.2252 214.6162 4

25 2872.3788 1436.6930 2855.3523 1428.1798 2854.3682 1427.6878 A 317.1932 159.1002 300.1666 150.5870 3

26 2943.4159 1472.2116 2926.3894 1463.6983 2925.4054 1463.2063 A 246.1561 123.5817 229.1295 115.0684 2

27 R 175.1190 88.0631 158.0924 79.5498 1

MUCIN 4

LIPOPROTEIN LIPASE (P06858)


1 102.0550 51.5311 84.0444 42.5258 T 30

2 199.1077 100.0575 181.0972 91.0522 P 3210.4103 1605.7088 3193.3837 1597.1955 3192.3997 1596.7035 29

3 328.1503 164.5788 310.1397 155.5735 E 3113.3575 1557.1824 3096.3310 1548.6691 3095.3469 1548.1771 28

4 443.1773 222.0923 425.1667 213.0870 D 2984.3149 1492.6611 2967.2884 1484.1478 2966.3043 1483.6558 27

5 544.2249 272.6161 526.2144 263.6108 T 2869.2880 1435.1476 2852.2614 1426.6343 2851.2774 1426.1423 26

6 615.2620 308.1347 597.2515 299.1294 A 2768.2403 1384.6238 2751.2137 1376.1105 2750.2297 1375.6185 25

7 744.3046 372.6560 726.2941 363.6507 E 2697.2032 1349.1052 2680.1766 1340.5919 2679.1926 1340.0999 24

8 859.3316 430.1694 841.3210 421.1641 D 2568.1606 1284.5839 2551.1340 1276.0707 2550.1500 1275.5786 23

9 960.3793 480.6933 942.3687 471.6880 T 2453.1336 1227.0705 2436.1071 1218.5572 2435.1231 1218.0652 22

10 1120.4099 560.7086 1102.3993 551.7033 C 2352.0860 1176.5466 2335.0594 1168.0333 2334.0754 1167.5413 21

11 1257.4688 629.2381 1239.4583 620.2328 H 2192.0553 1096.5313 2175.0288 1088.0180 2174.0447 1087.5260 20

12 1370.5529 685.7801 1352.5423 676.7748 L 2054.9964 1028.0018 2037.9698 1019.4886 2036.9858 1018.9966 19

13 1483.6370 742.3221 1465.6264 733.3168 I 1941.9123 971.4598 1924.8858 962.9465 1923.9018 962.4545 18

14 1580.6897 790.8485 1562.6792 781.8432 P 1828.8283 914.9178 1811.8017 906.4045 1810.8177 905.9125 17

15 1637.7112 819.3592 1619.7006 810.3539 G 1731.7755 866.3914 1714.7490 857.8781 1713.7649 857.3861 16

16 1736.7796 868.8934 1718.7690 859.8882 V 1674.7540 837.8807 1657.7275 829.3674 1656.7435 828.8754 15

17 1807.8167 904.4120 1789.8061 895.4067 A 1575.6856 788.3465 1558.6591 779.8332 1557.6751 779.3412 14

18 1936.8593 968.9333 1918.8487 959.9280 E 1504.6485 752.8279 1487.6220 744.3146 1486.6379 743.8226 13

19 2023.8913 1012.4493 2005.8808 1003.4440 S 1375.6059 688.3066 1358.5794 679.7933 1357.5954 679.3013 12

20 2122.9597 1061.9835 2104.9492 1052.9782 V 1288.5739 644.7906 1271.5473 636.2773 1270.5633 635.7853 11

21 2193.9969 1097.5021 2175.9863 1088.4968 A 1189.5055 595.2564 1172.4789 586.7431 1171.4949 586.2511 10

22 2295.0445 1148.0259 2277.0340 1139.0206 T 1118.4684 559.7378 1101.4418 551.2245 1100.4578 550.7325 9

23 2455.0752 1228.0412 2437.0646 1219.0359 C 1017.4207 509.2140 1000.3941 500.7007 999.4101 500.2087 8

24 2592.1341 1296.5707 2574.1235 1287.5654 H 857.3900 429.1987 840.3635 420.6854 839.3795 420.1934 7

25 2739.2025 1370.1049 2721.1919 1361.0996 F 720.3311 360.6692 703.3046 352.1559 702.3206 351.6639 6

26 2854.2294 1427.6184 2837.2029 1419.1051 2836.2189 1418.6131 N 573.2627 287.1350 556.2362 278.6217 555.2521 278.1297 5

27 2991.2884 1496.1478 2974.2618 1487.6345 2973.2778 1487.1425 H 458.2358 229.6215 441.2092 221.1082 440.2252 220.6162 4

28 3078.3204 1539.6638 3061.2938 1531.1506 3060.3098 1530.6585 S 321.1769 161.0921 304.1503 152.5788 303.1663 152.0868 3

29 3165.3524 1583.1798 3148.3259 1574.6666 3147.3418 1574.1746 S 234.1448 117.5761 217.1183 109.0628 216.1343 108.5708 2

30 K 147.1128 74.0600 130.0863 65.5468 1

CHORDIN-LIKE PROTEIN (Q6WN34)


1 88.0393 44.5233 70.0287 35.5180 S 24

2 248.0700 124.5386 230.0594 115.5333 C 2747.2089 1374.1081 2730.1824 1365.5948 2729.1984 1365.1028 23

3 376.1285 188.5679 359.1020 180.0546 358.1180 179.5626 Q 2587.1783 1294.0928 2570.1517 1285.5795 2569.1677 1285.0875 22

4 513.1874 257.0974 496.1609 248.5841 495.1769 248.0921 H 2459.1197 1230.0635 2442.0931 1221.5502 2441.1091 1221.0582 21

5 628.2144 314.6108 611.1878 306.0976 610.2038 305.6055 N 2322.0608 1161.5340 2305.0342 1153.0208 2304.0502 1152.5287 20

6 685.2358 343.1216 668.2093 334.6083 667.2253 334.1163 G 2207.0339 1104.0206 2190.0073 1095.5073 2189.0233 1095.0153 19

7 786.2835 393.6454 769.2570 385.1321 768.2730 384.6401 T 2150.0124 1075.5098 2132.9858 1066.9966 2132.0018 1066.5045 18

8 917.3240 459.1656 900.2975 450.6524 899.3134 450.1604 M 2048.9647 1024.9860 2031.9382 1016.4727 2030.9541 1015.9807 17

9 1080.3873 540.6973 1063.3608 532.1840 1062.3768 531.6920 Y 1917.9242 959.4657 1900.8977 950.9525 1899.9137 950.4605 16

10 1208.4459 604.7266 1191.4194 596.2133 1190.4353 595.7213 Q 1754.8609 877.9341 1737.8343 869.4208 1736.8503 868.9288 15

11 1345.5048 673.2561 1328.4783 664.7428 1327.4943 664.2508 H 1626.8023 813.9048 1609.7758 805.3915 1608.7918 804.8995 14

12 1402.5263 701.7668 1385.4997 693.2535 1384.5157 692.7615 G 1489.7434 745.3753 1472.7169 736.8621 1471.7328 736.3701 13

13 1531.5689 766.2881 1514.5423 757.7748 1513.5583 757.2828 E 1432.7219 716.8646 1415.6954 708.3513 1414.7114 707.8593 12

14 1644.6529 822.8301 1627.6264 814.3168 1626.6424 813.8248 I 1303.6793 652.3433 1286.6528 643.8300 1285.6688 643.3380 11

15 1791.7214 896.3643 1774.6948 887.8510 1773.7108 887.3590 F 1190.5953 595.8013 1173.5687 587.2880 1172.5847 586.7960 10

16 1878.7534 939.8803 1861.7268 931.3671 1860.7428 930.8751 S 1043.5269 522.2671 1026.5003 513.7538 1025.5163 513.2618 9

17 1949.7905 975.3989 1932.7640 966.8856 1931.7799 966.3936 A 956.4948 478.7511 939.4683 470.2378 938.4843 469.7458 8

18 2086.8494 1043.9283 2069.8229 1035.4151 2068.8389 1034.9231 H 885.4577 443.2325 868.4312 434.7192 867.4472 434.2272 7

19 2215.8920 1108.4496 2198.8655 1099.9364 2197.8814 1099.4444 E 748.3988 374.7030 731.3723 366.1898 730.3883 365.6978 6

20 2328.9761 1164.9917 2311.9495 1156.4784 2310.9655 1155.9864 L 619.3562 310.1817 602.3297 301.6685 601.3457 301.1765 5

21 2476.0445 1238.5259 2459.0179 1230.0126 2458.0339 1229.5206 F 506.2722 253.6397 489.2456 245.1264 488.2616 244.6344 4

22 2573.0973 1287.0523 2556.0707 1278.5390 2555.0867 1278.0470 P 359.2037 180.1055 342.1772 171.5922 341.1932 171.1002 3

23 2660.1293 1330.5683 2643.1027 1322.0550 2642.1187 1321.5630 S 262.1510 131.5791 245.1244 123.0659 244.1404 122.5738 2

24 R 175.1190 88.0631 158.0924 79.5498 1

LEUCINE-RICH α-2GLYCOPROTEIN 1 (P02750) By MALDI-TOF/TOF

Monoisotopic mass of neutral peptide Mr(calc): 1424.8391 Fixed modifications: Carbamidomethyl (C) Variable modifications: N8 : Deamidated (NQ) Ions Score: 41 Expect: 0.0069 Matches (Bold Red): 15/191 fragment ions using 22 most intense peaks

# Immon. a a* a0 b b* b0 Seq. v w w' y y* y0 #

1 86.0964 86.0964 114.0913 L 13

2 70.0651 183.1492 211.1441 P 1270.7154 1269.7201 1312.7623 1295.7358 1294.7518 12

3 70.0651 280.2020 308.1969 P 1173.6626 1172.6674 1215.7096 1198.6830 1197.6990 11

4 30.0338 337.2234 365.2183 G 1118.6568 1101.6303 1100.6462 10

5 86.0964 450.3075 478.3024 L 1003.5571 1002.5619 1061.6354 1044.6088 1043.6248 9

6 86.0964 563.3915 591.3865 L 890.4730 889.4778 948.5513 931.5247 930.5407 8

7 44.0495 634.4287 662.4236 A 819.4359 835.4672 818.4407 817.4567 7

8 88.0393 749.4556 732.4290 777.4505 760.4240 N 704.4090 703.4137 764.4301 747.4036 746.4195 6

9 120.0808 896.5240 879.4975 924.5189 907.4924 F 557.3406 649.4032 632.3766 631.3926 5

10 74.0600 997.5717 980.5451 979.5611 1025.5666 1008.5401 1007.5560 T 456.2929 469.3133 471.2926 502.3348 485.3082 484.3242 4

11 86.0964 1110.6558 1093.6292 1092.6452 1138.6507 1121.6241 1120.6401 L 343.2088 342.2136 401.2871 384.2605 3

12 86.0964 1223.7398 1206.7133 1205.7293 1251.7347 1234.7082 1233.7242 L 230.1248 229.1295 288.2030 271.1765 2

13 129.1135 R 74.0237 73.0284 175.1190 158.0924 1


PP 167.1179 195.1128 PPG 224.1394 252.1343 PPGL 337.2234 365.2183

PPGLL 450.3075 478.3024 PPGLLA 521.3446 549.3395 PPGLLAN 636.3715 664.3664

PG 127.0866 155.0815 PGL 240.1707 268.1656 PGLL 353.2547 381.2496

PGLLA 424.2918 452.2867 PGLLAN 539.3188 567.3137 PGLLANF 686.3872 714.3821

GL 143.1179 171.1128 GLL 256.2020 284.1969 GLLA 327.2391 355.2340

GLLAN 442.2660 470.2609 GLLANF 589.3344 617.3293 GLLANFT 690.3821 718.3770

LL 199.1805 227.1754 LLA 270.2176 298.2125 LLAN 385.2445 413.2395

LLANF 532.3130 560.3079 LLANFT 633.3606 661.3555 LA 157.1335 185.1285

LAN 272.1605 300.1554 LANF 419.2289 447.2238 LANFT 520.2766 548.2715

LANFTL 633.3606 661.3555 AN 159.0764 187.0713 ANF 306.1448 334.1397

ANFT 407.1925 435.1874 ANFTL 520.2766 548.2715 ANFTLL 633.3606 661.3555

NF 235.1077 263.1026 NFT 336.1554 364.1503 NFTL 449.2395 477.2344

NFTLL 562.3235 590.3184 FT 221.1285 249.1234 FTL 334.2125 362.2074

FTLL 447.2966 475.2915 TL 187.1441 215.1390 TLL 300.2282 328.2231

LL 199.1805 227.1754

FOLATE RECEPTOR 1(P15328)

PLASMINOGEN (P00747)

KALLIKREIN-12 (Q9UKR0)

LACTOPEROXIDASE PRECURSOR (P22079)

PROTEIN WNT-2B (Q93097)

CLUSTERIN (APOLIPOPROTEIN J) (P10909)

IMMUNOGLOBULIN J-CHAIN (P01591)

ZINC α-2 GLYCOPROTEIN (P25311)

GALECTIN 3-BINDING PROTEIN (Q08380)

MACROPHAGE MANNOSE RECEPTOR 1 (CD206) – (P22897)

CD36 PLATELET GLYCOPROTEIN 4 (P16671)

CD59 GLYCOPROTEIN (PROTECTIN) (P13987)

SER/THR PHOSPHATASE 2A SUBUNIT B” SUBUNIT α (Q06190)

FIBRINOGEN (P02679)

PHENIX – MS OUTPUT EXAMPLES LACTOFERRIN

POLYMERIC IG RECEPTOR

IG A-2 HEAVY CHAIN

LACTADHERIN

TENASCIN-C

In this file are reported MS/MS spectra from MASCOT and PHENYX search engines for the

identification of four peptides containing infrequent N-glycosylation sites (asparagines of Asn-

Xaa-Cys or Asn-Pro-Ser sites). As explained into the text, effective N-linked glycosylation,

especially at the Asn-Pro-Ser sites, would require adequate confirmation by other techniques.

CLUSTERIN (APOLIPOPROTEIN J) (P10909)


1 161.0379 81.0226 C 18

2 317.1390 159.0732 300.1125 150.5599 R 1919.9127 960.4600 1902.8862 951.9467 1901.9022 951.4547 17

3 446.1816 223.5945 429.1551 215.0812 428.1711 214.5892 E 1763.8116 882.4094 1746.7851 873.8962 1745.8010 873.4042 16

4 559.2657 280.1365 542.2391 271.6232 541.2551 271.1312 I 1634.7690 817.8881 1617.7425 809.3749 1616.7585 808.8829 15

5 672.3498 336.6785 655.3232 328.1652 654.3392 327.6732 L 1521.6850 761.3461 1504.6584 752.8328 1503.6744 752.3408 14

6 759.3818 380.1945 742.3552 371.6813 741.3712 371.1892 S 1408.6009 704.8041 1391.5743 696.2908 1390.5903 695.7988 13

7 858.4502 429.7287 841.4236 421.2155 840.4396 420.7235 V 1321.5689 661.2881 1304.5423 652.7748 1303.5583 652.2828 12

8 973.4771 487.2422 956.4506 478.7289 955.4666 478.2369 D 1222.5005 611.7539 1205.4739 603.2406 1204.4899 602.7486 11

9 1133.5078 567.2575 1116.4812 558.7443 1115.4972 558.2523 C 1107.4735 554.2404 1090.4470 545.7271 1089.4629 545.2351 10

10 1220.5398 610.7735 1203.5133 602.2603 1202.5293 601.7683 S 947.4429 474.2251 930.4163 465.7118 929.4323 465.2198 9

11 1321.5875 661.2974 1304.5609 652.7841 1303.5769 652.2921 T 860.4108 430.7091 843.3843 422.1958 842.4003 421.7038 8

12 1435.6304 718.3189 1418.6039 709.8056 1417.6199 709.3136 N 759.3632 380.1852 742.3366 371.6719 741.3526 371.1799 7

13 1550.6574 775.8323 1533.6308 767.3190 1532.6468 766.8270 N 645.3202 323.1638 628.2937 314.6505 627.3097 314.1585 6

14 1647.7101 824.3587 1630.6836 815.8454 1629.6996 815.3534 P 530.2933 265.6503 513.2667 257.1370 512.2827 256.6450 5

15 1734.7422 867.8747 1717.7156 859.3614 1716.7316 858.8694 S 433.2405 217.1239 416.2140 208.6106 415.2300 208.1186 4

16 1862.8007 931.9040 1845.7742 923.3907 1844.7902 922.8987 Q 346.2085 173.6079 329.1819 165.0946 3

17 1933.8378 967.4226 1916.8113 958.9093 1915.8273 958.4173 A 218.1499 109.5786 201.1234 101.0653 2

18 K 147.1128 74.0600 130.0863 65.5468 1

IMMUNOGLOBULIN α-2 CHAIN C (P01877)

LACTADHERIN (Q08431)

POLYCOMB GROUP RING FINGER PROTEIN 6 (Q9BYE7)

Fragmentation of the 3-charged ion (m/z=534.9381)

Fragmentation of the 4-charged ion (m/z= 401.4731)

Supporting Information for Proteomics DOI … · Supporting Information for Proteomics DOI 10 ... 8...

Documents

Transcript of Supporting Information for Proteomics DOI … · Supporting Information for Proteomics DOI 10 ... 8...