Supporting Information final - Ecography › sites › ecography.org › files › appendix ›...

History & adaptation on the CO Plateau – Massatti & Knowles 2020

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Ecography ECOG-04840 Massatti, R. and Knowles, L. L. 2019. The historical context of contemporary climatic adaptation: a case study in the climatically dynamic and environmentally complex southwestern United States. – Ecography doi: 10.1111/ecog.04840

Appendix 1 Additional methodological details: Field sampling and data generation Processing was accomplished using STACKS version 2.2 (Catchen et al. 2013) and followed the r80 protocol detailed in Rochette & Catchen (2017). Briefly, DNA was doubly digested with EcoRI and MspI restriction enzymes, followed by the ligation of Illumina adaptor sequences and barcodes. Each individual was barcoded twice using unique combinations of forward and reverse indexes. Ligation products were pooled and amplified using 18 cycles of PCR. A Pippin Prep (Sage Science, Beverly, MA, USA) was used to size select amplicons from 400 to 600 base pairs. Multiple 192-sample libraries, with 2 barcodes for each individual (i.e., 384 unique indexes per library) were sequenced on a HiSeq 4000 (Illumina, San Diego, CA, USA) at the University of Oregon’s Genomics and Cell Characterization Core Facility to generate single-end 100 base pair reads.

The process_radtags script was used to exclude raw reads containing more than four low-quality sites and adapter contamination. Parameters affecting the assembly were assessed based on how parameter combinations affected r80 loci (i.e., those found in 80% of samples or more); the optimal parameter set associated with the plateau of the number of r80 loci was selected (Paris et al. 2017; Rochette & Catchen 2017). Values used in the final assembly were: read to initiate a new putative allele (-m in ustacks) = 3; number of mismatches allowed between the two alleles of a heterozygote sample (-M in ustacks) = 5; mismatches allowed between any two alleles of the population (-n in cstacks) = 5. Results of this processing methodology are reported in Tables S1 and S2. The populations program in STACKS was executed to generate the datasets used in analyses under the settings: minimum percentage of individuals in a population required to process a locus for that population (-r) = 0.5, minimum minor allele frequency required to process a nucleotide site at a locus (--min_maf) = 0.05, maximum observed heterozygosity required to process a nucleotide site at a locus (max_obs_het) = 0.7, and the correction applied to FST values (--fst_correction) = p_value. Population structure and history


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STRUCTURE was run across K-values ranging from 1 to 9 without assigning population membership a priori. Twenty independent runs per K were conducted, each with 150,000 burn-in and 500,000 Markov chain Monte Carlo iterations, using an admixture model with correlated allele frequencies. STRUCTURE HARVESTER and DISTRUCT were used to visualize results, and the most probable K was chosen based on DK (Evanno et al. 2005). For sPCA analysis, geographic locations of individuals were created by jittering the latitude/longitude of their sampling localities (factor=3), and a Delaunay triangulation graph was used to create the required connection network. estimate

Demographic modeling to elucidate the number of refugial H. jamesii populations, the history and timing of population splits, and approximations of population sizes was conducted using the allele frequency spectrum method (Gutenkunst et al. 2009) implemented in FASTSIMCOAL2 (version 2603; Excoffier et al. 2013). This procedure uses coalescent simulations to calculate the likelihoods of observed allele frequency spectra (see Nielsen 2000) under user-specified demographic models. To improve the performance of the models by reducing the number of estimated parameters (Excoffier et al. 2013), one population parameter was calculated directly from the data. Specifically, the effective population size of the Western population (NWest) was fixed, whereas the other parameters (i.e., population divergence time, T, population sizes, Ne, and gene flow, 2Nm) were estimated based on the site frequency spectrum (see Fig. 3). The best-supported model was selected using Akaike information criterion (Akaike 1974). The effective population size of the Western population was calculated using the equation: Ne = (π/4µ), assuming a genome-wide single nucleotide polymorphism (SNP) mutation rate similar to Arabidopsis thaliana (7 × 10−9 per site per generation; Ossowski et al. 2010). Nucleotide diversity (π) was estimated from polymorphic and nonpolymorphic loci using STACKS (π =0.0063). One hundred runs per model were conducted and the global maximum likelihood solution is presented. Each run was performed with 200,000 simulations per likelihood estimation and 50 expectation-conditional maximization (ECM) cycles. Parameter confidence intervals were calculated from 100 parametric bootstrap replicates, by simulating site frequency spectra with the same number of SNPs from the maximum composite likelihood estimates and re-estimating parameters each time (Excoffier et al. 2013). Identifying putative adaptive loci Multiple LFMMs were generated using K-values (i.e., the number of latent factors) ranging from 3 to 5, which represent the most likely K genetic clusters suggested by STRUCTURE and sPCA, as well as closely related values (see Frichot & François 2015). The latent factors were implemented in LFMMs using a sparse non-negative matrix factorization algorithm, which produces results similar to those produced by STRUCTURE (Frichot et al. 2014) and were performed using snmf in LEA version 1.2.0 in R (Frichot & François 2015). Missing data were replaced by the most likely genotype using impute in LEA . For each K-value, LFMMs were generated for each of the three dominant environmental PC axes identified for the Colorado Plateau region. Models were run ten times with 30,000 iterations and a burn-in of 15,000. To assess model fit and genomic inflation (i.e., inflation of z-scores at each locus due to not sufficiently accounting for population structure or other confounding factors within the model), the median squared z-score calculated from ranked loci was divided by the median of the chi-squared distribution (Frichot & François 2015; François et al. 2016). Per locus z-scores were used to correct for inflation (Table S6), and putative adaptive loci were identified by considering


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the combination of the intersection of loci across K-values for each environmental PC axis (Table S6). References Akaike, H. (1974). A new look at the statistical model identification. In Selected Papers of

Hirotugu Akaike (pp. 215-222). Springer, New York, NY. Catchen, J., Hohenlohe, P. A., Bassham, S., Amores, A., & Cresko, W. A. (2013). Stacks: an

analysis tool set for population genomics. Molecular ecology, 22(11), 3124-3140. Evanno, G., Regnaut, S., & Goudet, J. (2005). Detecting the number of clusters of individuals

using the software STRUCTURE: a simulation study. Molecular ecology, 14(8), 2611-2620. Excoffier, L., Dupanloup, I., Huerta-Sánchez, E., Sousa, V. C., & Foll, M. (2013). Robust

demographic inference from genomic and SNP data. PLoS genetics, 9(10), e1003905. François, O., Martins, H., Caye, K., & Schoville, S. D. (2016). Controlling false discoveries in

genome scans for selection. Molecular Ecology, 25(2), 454-469. Frichot, E., Mathieu, F., Trouillon, T., Bouchard, G., & François, O. (2014). Fast and efficient

estimation of individual ancestry coefficients. Genetics, 196(4), 973-983. Frichot, E., & François, O. (2015). LEA: an R package for landscape and ecological association

studies. Methods in Ecology and Evolution, 6(8), 925-929. Gutenkunst, R. N., Hernandez, R. D., Williamson, S. H., & Bustamante, C. D. (2009). Inferring

the joint demographic history of multiple populations from multidimensional SNP frequency data. PLoS genetics, 5(10), e1000695.

Nielsen, R. (2000). Estimation of population parameters and recombination rates from single nucleotide polymorphisms. Genetics, 154(2), 931-942.

Ossowski, S., Schneeberger, K., Lucas-Lledó, J. I., Warthmann, N., Clark, R. M., Shaw, R. G., ... & Lynch, M. (2010). The rate and molecular spectrum of spontaneous mutations in Arabidopsis thaliana. Science, 327(5961), 92-94.

Paris, J. R., Stevens, J. R., & Catchen, J. M. (2017). Lost in parameter space: a road map for stacks. Methods in Ecology and Evolution, 8(10), 1360-1373.

Rochette, N. C., & Catchen, J. M. (2017). Deriving genotypes from RAD-seq short-read data using Stacks. Nature Protocols, 12(12), 2640.


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Figure A1. Individuals colored by genetic similarity when utilizing the first three global sPCA structures (sPC1, sPC2, and sPC3). Individuals are graphed in geographic space according to their sampling localities, and they are jittered so that they do not completely overlap. More similar colors represent more similar genetic identities. The inset shows the sPCA eigenvalues, and the magnitudes of the first three axes (colored red) support interpreting the first three global (positive) structures.


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Figure A2. Distribution of putative adaptive SNPs between standard deviations (z-scores) of 2 and 3. The inset shows how SNPs align with predictor axes (‘# RDA loci’), as well as how loci are shared with LFMMs (‘Loci shared w/ LFMMs’).

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Predictor # RDA loci (% total)Loci shared w/

LFMMs (% total)PC1 (elevation) 40 (6.1) 13 (9.9)PC2 (latitude) 338 (51.8) 71 (54.2)PC3 (MDR) 273 (41.9) 47 (35.9)Total 652 131


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Table A1. Details of sampling localities, climatic data, and processing. Columns indicate: total number of individuals sampled per locality (# Ind); sampling locality coordinates (Longitude/Latitude); elevation; extracted data for sampling sites from climatic PC axes

(PC1-3); average # reads per individual per sampling locality after data processing (Processed reads); average # reads per individual per sampling locality used to construct loci (Utilized reads). Sampling localities used in FASTSIMCOAL2 modeling are indicated using

bold text in the sampling locality column.

Sampling locality # Ind Longitude Latitude Elevation (m) PC1 PC2 PC3 Processed reads (± SD) Utilized reads (± SD)

North Rim 11 -112.710 36.767 1414 0.911 -1.707 1.072 1,245,496 (101,270) 1,052,456 (102,177)

Kaiparowits Plateau 12 -111.725 37.741 1996 -0.350 -1.228 -0.021 990,920 (55,765)

855,800 (49,511)

Lees Ferry 11 -111.621 36.852 1042 3.116 -3.754 -0.277 1,191,665 (142,060) 1,011,624 (125,478)

Red Mtn 11 -111.567 35.496 1915 -0.194 0.435 1.054 1,741,374 (558,394) 1,464,503 (476,134)

Alvey Wash 12 -111.498 37.692 1695 0.695 -2.322 -0.203 1,310,176 (179,545) 1,128,081 (156,875)

Poison Spring 10 -110.953 39.465 2053 -2.079 -2.562 0.265 2,042,384 (273,708) 1,747,805 (244,424)

Factory Butte 15 -110.878 38.417 1447 0.872 -3.506 -2.832 1,433,932 (118,685) 1,226,685 (112,636)

San Rafael Swell 11 -110.708 38.837 2051 -1.226 -2.328 -0.977 982,208 (84,813) 824,946 (77,268)

Blue Mesa 13 -109.787 35.090 1708 1.664 -0.366 -0.668 992,029 (82,857) 838,384 (73,855)

Sevenmile Canyon 11 -109.749 38.641 1514 -0.241 -3.411 -1.140 1,850,053 (295,026) 1,601,862 (258,100)

Needles 22 -109.726 38.182 1536 0.338 -3.198 -0.400 1,187,683 (116,470) 1,025,364 (105,854)

Vernal 11 -109.639 40.329 1524 -1.416 -4.535 -3.670 1,488,731 (138,928) 1,293,550 (130,305)

Combs Ridge 12 -109.606 37.293 1494 0.885 -3.034 -0.392 1,642,252 (210,874) 1,413,178 (185,278)

Book Cliffs 12 -109.482 39.725 1935 -2.139 -3.438 -1.451 2,013,492 (200,195) 1,729,377 (183,651)

Devil's Playground 10 -109.241 40.100 1569 -1.548 -4.301 -3.429 1,300,533 (107,935) 1,127,041 (100,973)


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Westwater 13 -109.142 39.121 1383 -0.445 -3.724 -1.615 1,036,647 (97,690) 867,980 (95,293)

Fruita Canyon 10 -108.696 39.060 1870 -1.622 -2.835 -0.034 1,825,923 (225,069) 1,559,224 (203,415)

Gypsum Gap 12 -108.663 38.041 1933 -1.464 -1.598 -0.201 1,276,019 (498,330) 1,084,938 (429,596)

Kutz Canyon 12 -107.990 36.658 1683 0.722 -2.312 -0.637 1,845,934 (234,880) 1,603,655 (215,686)

Coal Creek 24 -107.988 36.206 1921 0.382 -1.332 -0.819 1,203,728 (159,426)

1,038,926 (148,868)

Jesus Mesa 11 -107.671 35.380 2246 -0.460 1.119 -0.494 2,068,927 (175,901) 1,766,803 (144,279)

Manzanares 12 -107.640 36.716 1971 -0.781 -1.274 -0.100 1,057,901 (108,100) 883,206 (93,709)

Bear Mtns 11 -107.210 34.206 1880 1.508 2.347 -1.356 970,546 (68,974) 819,289 (62,349)

Box Canyon 12 -107.029 34.017 1822 1.577 2.466 -1.115 1,027,207 (59,734) 858,331 (59,724)

Hidden Mtn 10 -107.002 34.794 1546 1.885 0.168 -1.668 935,603 (51,808) 798,181 (43,525)

Rio Grande 25 -106.887 34.354 1482 2.170 0.880 -2.081 1,221,601 (116,738) 1,061,136 (110,235)

Black Mesa 12 -105.912 35.959 2004 -0.324 1.289 -1.250 1,408,209 (229,108) 1,219,357 (226,874)


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Table A2. Details of herbarium samples included in analyses. Columns indicate: herbarium from which specimens were loaned (RM = Rocky Mountain Herbarium, UTC = Intermountain Herbarium); the catalog number of the specimen within the herbarium (unacc =

currently unaccessioned within the collection); specimen details, including collector(s), collection number, date, state, and location information; the “population” to which each specimen was assigned for some analyses; the number of processed reads (Processed);

and the number of utilized reads (Utilized).

Herbarium Catalog # Collector(s) Collection

# Date State Longitude Latitude Population Processed Utilized

RM 929825 Lukas 9076 6/17/09 WY -106.896 41.679 WY Basin 1,267,381 1,059,990

RM 791212 Ward 758 6/17/96 WY -107.831 41.070 WY Basin 1,181,269 1,033,826

RM 799451 Ward 5500 6/17/97 WY -108.287 41.041 WY Basin 996,070 831,621



RM 633171 Fertig 18341 7/2/98 WY -110.050 41.672 WY Basin 1,056,775 875,823

RM 688829 Nelson & Refsdal

36187 7/3/95 WY -110.201 41.671 WY Basin 1,499,703 1,342,538

RM 688830 Refsdal 5287 7/13/95 WY -109.895 41.107 WY Basin 864,847 726,545

RM 688828 Nelson, Refsdal,

& Welp 35473 6/20/95 WY -109.550 41.281 WY Basin 1,317,543 1,133,349

RM 645101 Fertig 18236 6/16/98 WY -109.493 41.007 WY Basin 991,289 865,471

RM unacc Welp 6874 7/25/95 WY -107.176 41.892 WY Basin 972,972 801,378

RM unacc Nelson & Roderick

40405 6/18/97 WY -107.071 41.879 WY Basin 1,100,909 937,811

RM unacc Chumley 4667 7/15/96 CO -104.598 38.513 Plains 1,191,887 1,065,336

RM unacc Elliot 6139 5/27/99 CO -104.561 38.311 Plains 1,142,148 985,015

RM unacc Nelson & Kuhn 72484 6/23/07 CO -103.457 37.889 Plains 1,030,392 873,375

RM unacc Kuhn 4683 6/4/08 CO -103.876 37.895 Plains 1,405,592 1,206,793

RM unacc Kuhn & Rankin 3466 8/12/07 CO -103.989 37.690 Plains 1,326,933 1,109,570

RM unacc Elliot 11779 9/20/03 CO -103.513 37.765 Plains 1,010,921 826,411

RM unacc Kuhn &

Hemenway 5507 6/23/08 CO -103.652 37.580 Plains 1,306,040 1,132,200

RM 904007 Legler 11058 9/13/08 NM -104.866 36.784 Eastern Sangres 1,489,777 1,300,226


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RM 904010 Legler 7401A 8/9/07 NM -104.625 36.725 Eastern Sangres 1,516,117 1,360,183



RM 904012 Legler & Snow 3937 6/7/07 NM -104.578 36.487 Eastern Sangres 1,509,432 1,305,446

UTC UTC00212309 Hardy 845 5/18/93 UT -113.397 41.241 Salt Lake 1,226,130 1,045,090

UTC UTC00196123 Gilbert s.n. 5/24/86 UT -113.640 40.900 Salt Lake 1,523,410 1,334,816

UTC UTC00244955 Shultz, Toler,

Ward, & Marler 17601 6/10/98 UT -113.316 40.427 Salt Lake 833,914 722,955

UTC UTC00232651 Long 894 6/1/00 UT -112.464 40.495 Salt Lake 1,827,709 1,607,615

UTC UTC00209030 Clark 5366 5/25/91 UT -112.942 40.116 Salt Lake 1,114,819 907,270

UTC UTC00203343 Barksworth 4557 6/15/85 UT -113.047 39.653 Salt Lake 1,386,108 1,184,674

UTC UTC00216399 Curto & Smith 1310 6/13/95 NV -115.091 38.437 Western Great Basin 1,199,723 1,017,342

UTC UTC00226441 Pinzl & Work 12199 5/11/97 NV -114.323 37.860 Western Great Basin 1,072,470 945,008

UTC UTC00275644 Gust 1863 5/16/09 NV -114.993 37.722 Western Great Basin 1,190,589 1,016,966

UTC UTC00278019 Tiehm 16973 6/9/15 NV -118.297 39.599 Western Great Basin 1,530,955 1,328,262

UTC UTC00275314 Tiehm 16682 5/18/14 NV -119.052 38.700 Western Great Basin 1,718,475 1,510,195

UTC UTC00226603 Pinzl 11793 5/8/96 NV -117.870 37.821 Western Great Basin 1,242,184 1,097,859

UTC UTC00220400 Pinzl 11348 5/11/95 NV -117.711 37.724 Western Great Basin 1,314,426 1,125,008

UTC UTC00226912 Henderson &

Cholewa 7303 5/26/87 CA -117.279 36.402 Western Great Basin 1,624,090 1,465,391


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Table A3. Correlation between the PC axes used in RDA and the bioclimatic variables (Fick & Hijmans, 2017), longitude, latitude, and elevation. Correlations greater than 0.7 (i.e., |r| > 0.7) are shown in bold.

Climatic variables PC1 PC2 PC3

Annual mean temperature (Bio1) 0.96 0.25 -0.03 Mean diurnal range (Bio2) 0.46 0.44 -0.83 Isothermality (Bio3) 0.37 0.91 -0.49 Temp. seasonality (Bio4) -0.06 -0.98 0.07 Maximum temp. warmest month (Bio5) 0.86 -0.34 0.05 Minimum temp. coldest month (Bio6) 0.72 0.55 0.28 Temp. annual range (Bio7) 0.02 -0.87 -0.27 Mean temp. wettest quarter (Bio8) 0.14 0.25 -0.31 Mean temp. driest quarter (Bio9) -0.06 0.47 0.06 Mean temp. warmest quarter (Bio10) 0.91 -0.19 0.10 Mean temp. coldest quarter (Bio11) 0.79 0.64 0.04 Annual precip. (Bio12) -0.67 0.49 0.21

Precip. wettest month (Bio13) -0.18 0.91 -0.36 Precip. driest month (Bio14) -0.87 -0.12 0.26 Precip. seasonality (Bio15) 0.36 0.85 -0.65 Precip. wettest quarter (Bio16) -0.25 0.88 -0.33 Precip. driest quarter (Bio17) -0.83 -0.18 0.52 Precip. warmest quarter (Bio18) -0.26 0.87 -0.33 Precip. coldest quarter (Bio19) -0.54 0.10 0.68

Monsoonal precip. 0.27 0.85 -0.57 Longitude 0.03 0.42 -0.62

Latitude -0.45 -0.91 0.32 Elevation -0.81 0.40 0.03


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Table A4. Genetic summary statistics for the H. jamesii populations. Transitional sampling localities between the Southeastern and Northern populations are grouped separately (all

represent a mixture of genetic clusters, with the minor cluster composing >25% of the total

identity – see Fig. 1). Results are presented for variant nucleotide positions only and include:

observed heterozygosity (HOBS), expected heterozygosity (HEXP), nucleotide diversity (π), and average Wright’s inbreeding coefficient (FIS).

Population HOBS (± SD) HEXP (± SD) π (± SD) FIS (± SD) Eastern 0.103 (± 0.006) 0.157 (± 0.011) 0.168 (± 0.010 0.171 (± 0.034)

Transitional 0.106 (± 0.003) 0.164 (± 0.009) 0.174 (± 0.010) 0.188 (± 0.021)

Northern 0.107 (± 0.008) 0.158 (± 0.009) 0.168 (± 0.008) 0.160 (± 0.036)

Western 0.103 (± 0.015) 0.153 (± 0.007) 0.165 (± 0.005) 0.155 (± 0.028)


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Table A5. Pairwise corrected AMOVA FST calculated between the sampling localities. All values are significant at p < 0.05. See Table S2 to determine which herbarium specimens were grouped to form sampling localities outside of the Colorado Plateau. Green cells indicate FST less than 0.030, yellow cells indicate FST between 0.030 and 0.049, and red cells indicate FST greater than 0.05.

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Bear Mtns 0.024 0.026 0.068 0.016 0.033 0.043 0.056 0.043 0.061 0.051 0.025 0.059 0.021 0.026 0.059 0.059 0.045 0.048 0.022 0.054 0.060 0.026 0.053 0.061 0.026 0.033 0.028 0.016 0.039 0.025 0.035 Kutz Can. 0.018 0.059 0.024 0.022 0.032 0.046 0.038 0.056 0.046 0.017 0.050 0.016 0.014 0.054 0.054 0.031 0.042 0.012 0.049 0.053 0.017 0.048 0.056 0.017 0.027 0.023 0.021 0.034 0.020 0.031 Combs R. 0.050 0.025 0.028 0.026 0.038 0.029 0.040 0.028 0.012 0.041 0.020 0.020 0.035 0.035 0.035 0.035 0.018 0.034 0.036 0.021 0.029 0.039 0.020 0.020 0.016 0.024 0.026 0.015 0.023

Book Cliffs 0.056 0.067 0.062 0.064 0.046 0.075 0.065 0.050 0.064 0.060 0.060 0.072 0.072 0.081 0.066 0.058 0.069 0.077 0.061 0.067 0.079 0.061 0.039 0.038 0.055 0.044 0.037 0.037 Box Can. 0.037 0.037 0.047 0.040 0.050 0.045 0.025 0.051 0.017 0.024 0.052 0.051 0.040 0.048 0.023 0.047 0.053 0.022 0.045 0.048 0.020 0.029 0.026 0.022 0.035 0.025 0.031

Coal Creek 0.042 0.056 0.050 0.063 0.057 0.026 0.057 0.024 0.021 0.064 0.065 0.039 0.052 0.019 0.059 0.064 0.024 0.059 0.065 0.024 0.038 0.034 0.031 0.044 0.032 0.041 Fruita Can. 0.053 0.041 0.058 0.045 0.024 0.056 0.034 0.033 0.054 0.054 0.051 0.047 0.031 0.051 0.054 0.035 0.048 0.059 0.035 0.033 0.029 0.035 0.038 0.023 0.034

Devils Play. 0.037 0.064 0.052 0.039 0.051 0.048 0.048 0.059 0.058 0.068 0.055 0.046 0.057 0.063 0.049 0.054 0.064 0.047 0.030 0.029 0.046 0.031 0.025 0.028 Factory 0.049 0.041 0.031 0.035 0.036 0.038 0.047 0.045 0.054 0.047 0.037 0.044 0.051 0.037 0.042 0.050 0.038 0.018 0.020 0.041 0.022 0.019 0.019 W. GB 0.035 0.044 0.065 0.056 0.057 0.037 0.035 0.078 0.061 0.054 0.030 0.046 0.060 0.034 0.015 0.057 0.041 0.038 0.052 0.046 0.037 0.042

Alvey 0.034 0.054 0.045 0.046 0.019 0.017 0.065 0.051 0.045 0.026 0.023 0.045 0.015 0.037 0.044 0.034 0.030 0.046 0.040 0.029 0.037 Gypsum 0.042 0.018 0.018 0.042 0.041 0.034 0.036 0.016 0.040 0.041 0.019 0.035 0.046 0.019 0.023 0.018 0.023 0.027 0.015 0.025

Poison 0.051 0.051 0.062 0.061 0.074 0.058 0.048 0.059 0.067 0.054 0.057 0.068 0.052 0.028 0.029 0.048 0.032 0.029 0.029 Hidden Mtn 0.017 0.054 0.054 0.035 0.044 0.014 0.049 0.054 0.017 0.047 0.057 0.018 0.027 0.023 0.016 0.033 0.021 0.030 Jesus Mesa 0.055 0.056 0.031 0.043 0.013 0.051 0.054 0.017 0.049 0.058 0.018 0.028 0.024 0.022 0.034 0.021 0.031

Kaiparowits 0.020 0.074 0.058 0.053 0.026 0.029 0.055 0.020 0.039 0.053 0.039 0.037 0.053 0.046 0.036 0.042 Lees Ferry 0.074 0.056 0.054 0.026 0.027 0.055 0.016 0.037 0.054 0.039 0.035 0.053 0.045 0.035 0.042

Manzanares 0.060 0.030 0.069 0.075 0.036 0.068 0.079 0.037 0.043 0.041 0.038 0.051 0.036 0.047 Needles 0.041 0.053 0.059 0.043 0.052 0.062 0.044 0.036 0.034 0.046 0.041 0.033 0.039

Black Mesa 0.048 0.053 0.015 0.047 0.055 0.015 0.026 0.022 0.019 0.033 0.020 0.029 North Rim 0.036 0.049 0.027 0.032 0.050 0.035 0.034 0.048 0.042 0.033 0.039 Blue Mesa 0.055 0.021 0.046 0.053 0.044 0.040 0.053 0.051 0.038 0.046

E. Plains 0.049 0.063 0.017 0.028 0.025 0.021 0.034 0.021 0.031 Red Mtn 0.037 0.048 0.033 0.030 0.047 0.040 0.030 0.036

Salt Lake 0.063 0.042 0.038 0.052 0.047 0.038 0.043 E. Sangres 0.029 0.023 0.021 0.034 0.022 0.030 San Rafael 0.014 0.030 0.017 0.012 0.013 Sevenmile 0.027 0.016 0.011 0.014

Rio Grande 0.036 0.026 0.033 Vernal 0.015 0.014

Westwater 0.012


13

Table A6. Latent factor mixed model results. For each environmental PC axis, the genomic inflation factor (GIF), number of candidate loci (# loci), and the percentage of the total loci included in the analysis (9534) is reported. Intersection refers to the number of loci in common for a PC axis across K values 3, 4, and 5.

LFMM K PC axis LFMM GIF # loci % of total loci 3 1 1.3 231 2.4 4 1 1.15 194 2.0 5 1 1.1 213 2.2

Intersection: 139 1.5 3 2 1.8 216 2.3 4 2 1.6 200 2.1 5 2 1.5 233 2.4

Intersection: 164 1.7 3 3 1.8 212 2.2 4 3 1.55 213 2.2 5 3 1.45 268 2.8

Intersection: 150 1.6 Total intersected loci (PC1+PC2+PC3): 453 4.8 Duplicate loci: 42 0.04 Total unique candidate loci: 411 4.3


14

Table A7. RADseq loci that matched significantly to NCGI’s nr database (i.e., to a known graminoid sequence and with an E-value ≤1x10-3). Shown are results of the subsequent blast to the UNIPROT database.

Locus # Sequence UNIPROT name UNIPROT identifier E-value %

identity Proposed function GO identifier

39572 AATTCTCCCATGGTGTATTTGAGAGCTGGCTCCATCCGTCCCAGTACCGCACTAGGCTCTGCAAGGAGG

GAGCAGCTTGCGCCCGCCGCATCTGCTTCTTT

Zinc finger CCCH domain-containing protein 50

Q84SL2 6.70E-17 100 DNA/metal ion

binding NA

10241 AATTCCTTCTTGAGATCCTTGAGGATCTTGTTGTAGCTGAACTCTTTCTTCAGTCCCTGGACAGTAGTCA

GACTCTTCCTGCCGTTGCGTTGCTGGATGCG

Protein translation factor SUI1 homolog 2

NA 3.00E-15 100 translation

initiation factor

activity

NA

34879 AATTCTCTTGCCGTCGGGCCGAACACGCGGGTATCCTTTGTGGCACACGGCTGATAGCCGAACAGCAT

CTCGCCCTTCTTCACCTGGAACACAGCGTCGTG

Allene oxide synthase D3K2N2 7.90E-15 93.9 oxidoreductase

activity NA

25038 AATTCTGTATCGTCTCCGCACGCGGCCTAGGCCGCAGATCATCACTGCTCAAACAGCAGTGGTTCTCA

GTCGCATGGATTGATCCAAACAGCAAATACTGC

uncharacterized protein A0A3L6FGA1 9.10E-15 87.9 NA NA

21473 AATTCTTAAGAACATCCCATGCCTTATCCAGAAGCCATTCTGAGTCTGCATTAGGTAGCTTGCGGACA

CCAGCTGTAGCATATAGAAAAACTGGAGTGTGT

Putative apyrase 7 A0A3L6RB81 1.30E-14 93.9 hydrolase

activity NA

9358 AATTCGGAGATCTTGGCTATGTGGCACCAGAGTATGCACGCACTCTGATGGCCACGCCGAAGGGTGAT

GTCTACAGCTTTGGTGTGGTTCTGCTTGAGATC

Putative inactive leucine-rich repeat receptor-like

protein kinase

A0A1E5V3C4 1.30E-14 97 ATP binding NA

22564

AATTCTATCTGCATGGGGAAACTTGCAGTATGCG

CATAGCATTGCACGGATGTGCACAATGTCAACCTTTCCAGAATGCTTGCAAGCAAGCTCATAAGCTG

uncharacterized protein A0A0A9JBL9 1.80E-14 90.9 NA NA

38340

AATTCGGCACAAGACCCTTGTACAGAGCACCAAC

ACCCTCATGACGAACAGTTTTCCTGAATGCATCGATCATACCATTGTACTGGAGCGCATCTTTGCCT

Mitochondrial adenine

nucleotide transporter ADNT1

A0A3L6E8A9 2.00E-14 97 transport NA

3428

AATTCCCAGAGCAGGAGGAGATGGGCTTGTGCTC

GTGCTGCTGCCGCTGCCTGGAGCTCCTGTGCTCCGTCCTCCTCCCGCCCCTAGGCGTCTGCCTCCGC

Low temperature and salt responsive protein-like

A0A3L6S273 2.80E-14 100 NA NA

38100

AATTCAAACCAGATCTCATAATGGTTAAACAGGA

AACTATCATGAGTGTGCTTCCCTACATACTCAAGCCACAATTTCGCAAGCCGCCGTGCGCACTCATC

uncharacterized protein A0A0A9CYF5 2.80E-14 90.9 NA NA

29981 AATTCCTGCGTAAGGAATCCCTGGCTCGCCAGCAGCAATATCAGCAGGCCAGCATGAGCCATTATGCAAATAATGCCGTGCCTGGGGACCAGCATGGTTAT

uncharacterized protein A0A0A9V7X2 4.10E-14 93.9 NA NA

21649 AATTCCCAACAACTCTGTATTACATGTTCAACACGATGCTTCTCACACTATTCGTGTTCCATGTGTACTG

GGGGAAACTCATATTTTTGATGATAAAGAAA uncharacterized protein A0A453IFS2 4.50E-14 90.9 NA NA

50123 AATTCAAGATGTCCAGATGAAGTTGTTAGGAGAATTCGTGTGAGTGTTTCATCTTACGATCCAGTTTGG

CAAGGAAAGCTACTGGACACTTATGACACTCA uncharacterized protein J3MFW0 7.40E-14 90.9 NA NA


15

33684 AATTCATACAGTCAGAAGATATCCTTTACAAGATGTTGTTGCTCTTGACAGCTCAAATATCATTGGAAT

TGAAGGTTGCAGTACCAGTGACATAAGGTCTG

uncharacterized protein A0A0A9DWL0 1.20E-13 97 NA NA

16768 AATTCCTTTCTCTGCCATGACGCCCACCTGTACAAATTCCATAAAAATCTTGCGTTTCTCACTTCTACTG

TGCCAGGTCCAAACCCAATTAAAATGTCAAG

uncharacterized protein A0A3B6HMF0 1.60E-13 84.8 NA NA

31062

AATTCTTGGTCAGGCTGTTTGCTGGAGTCAAGCTT

CTTCACCGCAGACTTGTTGCCGTCTTTCAGCACACCAAAGTAGACTCTGGCGTACGACCCTTCTCC

PTI1-like tyrosine-protein

kinase 3 A0A1D6MSA8 1.80E-13 97

protein

serine/threonine-protein kinase

NA

31748

AATTCCCCAAGAGCACCGTTGGACCTGGTGTGTG

GGGAGCGCTGCTTGGTGCTTGCATGGTCCATAAGGATAGCGACCATGCGAAACTGGCATCCCAGAAG

Pentatricopeptide repeat-

containing protein A0A1E5V3I1 2.20E-13 93.9 zinc ion binding NA

36632

AATTCCTACACGAACATTACTTCCATACTCAACTG

GTTTAAAATTTGCAGCTTTGAGTTCCTTTATAACACAGTCACCAATATGGAGGAAAGGAACCGAGC

Asp/Glu racemase CB6TUV7 3.40E-13 87.9

cellular amino

acid metabolic process

NA

4790 AATTCGTGTGAGTGTTTCATCTTACGATCCAGTTTGGCAAGGAAAGCTACTGGACACTTATGACACTCA

GAGTGATGTGTTCCGAATTGCACCTGCTTGCT uncharacterized protein A0A0A9CAP4 5.20E-13 87.9

protein-N-

terminal asparagine

amidohydrolase

activity

GO:0008418

34446

AATTCCCGTAGAGAATATTTCACCATCTCATTGTC

GAAATCAGCGCCACCAAGGTGAGTGTCACCAGCGACGGCCTTCACCTCGAAGACACCCATGTCAAT

uncharacterized protein A0A0E0R547 5.60E-13 90.9 ATP binding NA

20650

AATTCTTGCAGATTGTGCCTGCTTTTCTAGGCGGT

AAATGCAGCTGCTCACAATGCGAGAAGCCTCATGATAGCTCAGCAATACAGGCAGGTGAAGGGAGC

uncharacterized protein A0A0E0D8U7 8.00E-13 87.9

integral

membrane protein

GO:0016021

36264

AATTCAGGGGAGGTGTCTGGGACGACGGCGACGC

TGGTGGTCATCGACGGATTCACGGTCACCGTCGCCTCGGTGGGAGACTCCCGCTGCATCCTGGACAC

uncharacterized protein A0A453AL85 8.90E-13 93.9 catalytic

activity GO:0003824

26965

AATTCACTTGAGGAACGGCTTCTTGAAAGCATGG

CATGGGAGAAAGGCTCATCAATATATAATTCCTTGATTGTTGCTAGGCCAGCACTGTCTTCAGAAAT

Retinoblastoma-related protein 3

A0A1E5WFS4 1.40E-12 97 regulation of

cell cycle NA

46655

AATTCAAAATAACAAACCTCAGGTATGGAGCTAA

GTAGACGGCCGCGTAGACCAGATACCGCCGCTTCCCGCTATCGAGCCACGCAAAGATCCAGCTCTAT

uncharacterized protein A0A1D6JBS6 1.60E-12 90.9 NA NA

31807 AATTCTGATCTCTTCTATGCTGCACTTGGTGGTCTCGGTCAGTTCGGGATCATCACCAGAGCAAAGATT

GCCCTTGAACCTGCTCCAAAGACGGTAAGAAA Cytokinin dehydrogenase 4 Q5JLP4 2.00E-12 93.9

cytokinin metabolic process

NA

27402 AATTCTCCGCTGGCGCATCGGGTAGCTTGAACTCAGGGAAGTCATTGGATGGATTCGGTTGCTCGGAAGCTGCGACGGGCGACGATGGCAGGGCATTATCG

uncharacterized protein A0A0D9W1J4 2.80E-12 87.9 positive

regulation of GTPase activity

GO:0043547

25969 AATTCGGTTCAACAATCCATGCAATCACTACTCCAGCAACCTACACAGTCTGTTTTGAGGCAACAGCAACATCCACAGCCCATGCATCAGCAGCATTCTCT

Uncharacterized protein J9QIL5 3.70E-12 90.9 NA NA

31229 AATTCTGAAGGAAAGGCACTTGGTTTTACATTTTCCTTTCCCGTTAGACAAGCTTCTATATCCTCAGGGT

CATTAATTAGGTGGACTAAAGAATTTTCAAT Phosphotransferase A0A2S3HCZ7 3.70E-12 96.8

cellular glucose homeostasis

NA

4666 AATTCACAGTACAAGTCATCTTATCCAGCCAGCAGGGCAAGTGCAGATGACATTGAGCTGTATCCCCC

S-acyltransferase A0A0D9W8N7 5.10E-12 90.9 Acyltransferase NA


16

GACACCGAGCAGCTTCCAGAGCAACTCGCGGAC

19396 AATTCTTGGAGCAGCCTCCTTGTGATTCTAGGACCCTGCTCATAAGCATATTTATCCCAGATTTGGGTTC

AGATGGCATCTCCTTTGAAACTTTCCGGGAT uncharacterized protein K4A4W1 5.10E-12 87.5

electron transport chain

GO:0022900

28662 AATTCAAGGAGTTCGTTGTATGTCTGTTGTAGCTTGCCACTGTTGGTATTCATCTCCAGCAACTCATGTT

CATGCTCACCTAATTTTGCCTGGAGATCCAA

V-type proton ATPase

subunit a A0A446Q4H2 6.50E-12 87.5

proton transmembrane

transporter activity

GO:0015078

4693

AATTCAGAGGAACCTTAACCTTTTTTCCATCCTGC

TGGTTCCCCATGATGCTTGCATCAACTTCCACAGACCCGTTATTAATTTGAAGCATCTGCAAAACC

uncharacterized protein NA 6.70E-12 96.8

vesicle docking

involved in exocytosis

NA

40735

AATTCTTTCCTATGGCAGGGGTATGAGATTACTGT

CATATAATACAGTTGATATCAGGCTATCTGATCACCGCCCTGTGACTGCGGTGTACATGGTCGACG

Type 1 inositol 1,4,5-

triphosphate 5-phosphotase 1-like isoform X1

A0A3L6STM4 6.90E-12 87.9 hydrolase activity

NA

20565

AATTCCCGGCAGAGAGATAGAACGACGGCATTGT

ACGAGGCGCACACAGGCGGTGACACGGCCAGGACCTCCCTGGCCTCGTCCACCCTGCCAAGCTTGCA

uncharacterized protein A0A368QBL2 6.90E-12 87.9 NA NA

34675 AATTCTAGTTCCATCTGCACCACCAGAATCTCCCAAGTTAAAATACTTGAAGATTTCATCCCTGAAAGA

TAGCACATGTCTTGTTACTAATGGTGTCAGCA

RING-type E3 ubiquitin transferase

A0A2S3I7T6 7.00E-12 87.9 protein

ubiquitination GO:0016567

30274 AATTCCCGGGTACAATACCATTTACCCTAAAGGGACCTTCCCAATTCGGCAACCATTTGCCGAATTTAT

TATCTTTTCGCCCCAAAGGCAAAATCATTTTC uncharacterized protein J3NCY2 9.60E-12 72.7

nucleic acid binding

GO:0003676

30940 AATTCAGAAGAACACCCACTTGGACGCCGTCTTT

TCCTTCATGGTTTTGGAGTGGAAGAGCTCGTCGCCGTCGTCGCCGGCCTGCCGCCGCTTCTCCGCCC

uncharacterized protein A0A3L6TFT8 1.20E-11 93.8 NA NA

30173 AATTCAGCATTTTGAGAAGTTTGTCAAGGAGCACTTCACTTACCGCGCCCCACGCATTCTGGATGCCTG

CGAGGCTTATCTTAGTGGTGACCTTGTTGGAC

Putative ubiquitin-conjugating enzyme E2 24

A0A1E5VUQ7 1.30E-11 90.6 NA NA

3521 AATTCCTGCTCTTCTTCCTTAGATTGACCCGCGCCACCGCTACGGCCACAATTTGCATCTGTATTACGA

CATCTGGTCCGCGAGCTCGAGCACTGAGCCCT Calmodulin-binding protein B1B5J2 1.30E-11 100 NA NA

35565 AATTCAAGAGCGAGGAGGCGCTCATAGTCATCCATGTCGCTCTCCTATGCACGCAGGGCTCGCCTCACC

AGCGCCCACCAATGTCCAGGGTCGTGGCGATG

Putative LRR receptor-like serine/threonine-protein

kinase

A0A1E5W7S0 1.30E-11 90.9 protein

phosphorylation GO:0006468

11645 AATTCGTGAACATACTTGGGTCGGTGGTGGAGATGGTGGCGGCGCCATTGAAGTCGCAGGTGGCGCCT

TTGGCCTTGTTGTTCTGGTAGTAGCTGTTGGCG

PLASMODESMATA CALLOSE_BINDING

PROTEIN 1-like

A0A3L6S547 1.80E-11 93.1 NA NA

15561

AATTCTTGTCTCACTTTTTTGCAGGGCTGGCAGCG

CAAATTGGATCCAGATTACAATGTTATGAAAACATTACAGACTCTACTTTTCAAAGAAGACTGGGC

Mediator of RNA

polymerase II transcription subunit 7

A0A0E0KW91 2.20E-11 96.3

regulation of transcription by

RNA polymerase II

NA

35405

AATTCGGCGGCTGCGGAGGCCGCCCCAACGCCGA

CGAGCATGGGGTTGCGACCGCGGGAGAGGATGTCAGCGATTCGGCGGCAGTTATCCTCCCCTGCTCC

Protein DWARF 53 Q2RBP2 2.40E-11 93.5 protein binding GO:0005515

32666 AATTCTCCATTCTCGGCCCTATGCGGTCGGAGATATGCATTGATTGGCTGCATACATGCGATTGTAAAA

GCTATGATACCAATTCTAGCGTGTTTGGATAT uncharacterized protein A0A287XV68 3.30E-11 84.4

oxidation-reduction process

GO:0055114


17

33000 AATTCATACCTTTTCAGGCTAAGAACTTCATAGTTTTTCCGCAAATAATCTGCATGCTTAGTAAGAGCG

TCCTGGGCATAATCTTTGGCTACAGTTCGAAT

5'-3' exonuclease A0A3L6FDQ9 4.40E-11 87.5 exonuclease

activity NA

17452 AATTCTCTCAATTTGATCTCCTCTTCCTTGGTAGGAGCCATCTTTAGCAAGGTCTCCAGCAATTCTGCTC

CAAAGTTGCCTGTGTTACCTGCAGGACAAAC

Formin-like protein NA 4.50E-11 87.9 NA NA

33450

AATTCTGATTGTGCAGGATTTAATTGACATGGAC

ATGGAAGATGAACGGTCTGGGAGAATGGATGAAATTCACCAACGACTCCATTCTGCTTATTGCCAGT

Hyperosmalolality-gated

Ca2+ permeable channel 2.1 A0A097NUT4 6.30E-11 93.1 NA NA

25104

AATTCTCTGTATATGTGCATGGAGTGGGGCGTGG

CGATGGAGGTCGGCGACGATGTGCGGCGTCACGTGGTGGAGAGCAGGATAAGGGAGGCCATGGCCGG

uncharacterized protein A0A3B6AR37 6.40E-11 81.3 transferase

activity GO:0016758

22770

AATTCATGGTTCACCCACTTTCTAAGGCTCAGCTC

CTCGTTGAACATGGTGTCGGTGGGCTTCCTCCCCGTGATGACTTCAAGAAGCATTATCCCGTAGCT

Putative LRR receptor-like

serine/threonine-protein kinase

A0A1E5WK78 8.50E-11 87.1

integral

component of membrane

GO:0016021

24077

AATTCCCGGCCATACAATTCAGAGATATTGTCAC

GGCGACAAACAACTTCTCAAGTACATGTCTGATCGGCCGCGGAGGTTTCGGCAAAGTTTACAAGGTA

uncharacterized protein A0A3L6T804 8.60E-11 81.3 recognition of

pollen GO:0048544

30664 AATTCGGTAGCTGGGCTGCCATGACGGCACGTACCTTGCCGGCCTTTCCTGGTCTGGCCTTCCTTCCTG

CAGCTGTTGTCCCATAGGTGGGCCTCGTACCT Os06g0658000 protein A0A0P0X020 9.90E-11 85.2 NA NA

39055 AATTCTCAGGACAAGATGGCGTGTCGACCATCGAGCATATAAGCCAGTTCCTGGCTCAGTGCGGGGAGGCATCGACTGAAGATGCGCTGAGGGTGAGATTC

retrotransposon protein Q75HS0 3.00E-10 87.5 DNA

integration GO:0015074

14390 AATTCTGCGAACGCCAGGGCGCCGTACTTGTTGCCGAGCATGCCGTGCAGCTTTATGATGGTCTCCTCC

TCCTCGGCGGTGAAGTTGCCGCGCTTGAGGTC

Myb-related protein Zm1-like

NA 4.00E-10 96.4 DNA binding NA

25013 AATTCACTCTCTGATAGTGTGTCCAATTTGACGATGGATGATGCTACTTCAAACGCCGCACCTGGTGAA

AATGGGAACGGTGTGGCAGGATCCTGATTGTA uncharacterized protein C5XZU7 5.60E-10 87.1 actin binding GO:0003779

25040 AATTCGGGCTCACCTTGAGCTCGGGTAGGAAGTCGGTGAGGTGCGGGACGTCCTCGAGCACGTAACCG

CCGCAGCCGCTGGCGAGCTTCTTCTTGGCAGAC

ATP-dependent 6-phosphofructokinase

C0PCM8 7.60E-10 92.6

fructose 6-phosphate metabolic

process

NA

22453

AATTCGAGAAGGAACGTCGTACTTGTGGGAAGCG

ACGTGGAGCTCCTCGTTCTTGGAGCCTTTGAGGTTCTCGGAGCCGAGCTTCACGAGGAAGGAGCGCC

SNARE-like superfamily protein

NA 7.70E-10 100

retrograde

vesicle-mediated transport

NA

41687 AATTCAAGCCGAAGCTTTCAGACTTTGGCCTGGCGAGGGAGGGGCCAACCGAAGGCAGAACACACGTCTCCACCGCGGTAACTAACAACACGAGATCTCCT

uncharacterized protein A0A0Q3JIL9 1.40E-09 100 protein kinase

activity GO:0004672

28458 AATTCCCGAGGTTCAACTGAGGCAATGGCGGGCGAGCAGGTGCACGTGCTCTCTGCGCTGGACGGCGCCAAGACGCAGTGGTACCACTTCACGGCCATCAT

uncharacterized protein NA 1.90E-09 96.2 NA NA

4621 AATTCCTCATGCTCGAGGGCGAGGACGGCGAAGGGGGCTACTACCCCGAGCTACGCTGCTACGACGGGCAGTACTACTACGTGCAGGAGCAGCAGGAGGAG

uncharacterized protein A0A0E0KGX1 2.00E-09 75 NA NA

9868 AATTCTATTATTTCGGCAAGGGAACGAACTGGAGAGTAAGACAAATCTGACAAATAAGCGTTCAGGCT

uncharacterized protein I1IVI9 2.00E-09 96.7 NA NA


18

TAACTTGAACTCTGCTAATAGAGCAACCAGCTC

34818 AATTCCACTTTGACAACCTCAAGAAAGTATCTAT

CACTGGATTCTGTTCCTCCAAAAGCTTGATTGAGCTAACGGGTCAAATTCTTAAGACTTCCAAGTCT

uncharacterized protein A0A0A9RTP3 2.70E-09 81.8 NA NA

60433 AATTCCTACACTTGCCGTGCCAGCAACCAGAACACCCGTTAGCTCCGATGGATGTGGTTGCTACATGTT

GACTAACAGAGTCCATGTCTACCCGCGCCACA

uncharacterized protein A0A0A8YPK1 2.70E-09 76.7 RNA binding GO:0003723

31873 AATTCACGTCAACAAAGGTGGTACCTTTCCCAACTGATCGAAGAGCACTGAGGGGCTGCCATAGAGCT

GAGAAGGTGATTCCGATGCTGAACAAGTGGACC

uncharacterized protein A0A0A9NLR2 3.00E-09 100 integral

component of

membrane

GO:0016021

60473 AATTCTTAATCAACTAACAAGTAGCTGGTTCCAC

ATCTCCACGGCTTCTTGTCTCACCTTCTTCTTCCTC

CAGTACCCACTCATAACAACATCCCATGTTT

uncharacterized protein A0A2S3HTJ6 3.60E-09 82.8 NA NA

29577 AATTCCATCAGTTGAAGGGGCAGCATGACAAGCTCTTGAATTGACTCTTTGATATCATCTAGAGATCCA

ATGTCATCAAATGTCACTCCTATTTCACCAGC

Putative AAA domain-containing protein

C24B10.10c

A0A199W7S5 3.60E-09 86.7 NA NA

37250

AATTCTATCCAATCGGTATTTGCAGTGGCGGTGTG

TCAATGCACAGGCTGATGCTGCCCTTGCTGCGCAGAAGCTGACTGTTGAGGTGAGTATCTGCATTC

uncharacterized protein A0A2T7C0P8 3.70E-09 92.9 NA NA

147

AATTCTAATTCTTACATGGCGTGGCTCGACAGGC

AGCCGGCGCGTTCCGTGGTGTATGTAAGCTTCGGCAGCCGTAAGGCCTTGGCCAGGGAGCAGATAAG

Glycosyltranferase A0A0E0A8A8 3.70E-09 75.8 transferase

activity GO: 0016758

5622

AATTCTCATTCTCCTCCTCCTCCCCCTCCTCTTTGT

CCTCGTTCACATTCTCCAAAGCCTCCAAATCAGGCGGCCGAGGCGACGGTGGGGCAGGTACCGCC

uncharacterized protein A0A3L6QR23 3.70E-09 81.8 nucleic acid

binding GO:0003676

41751

AATTCCGAGCGATTTCACACAAGTCGCATGAACT

CAGCTTCCAAAGCTGCCAACAGGAACAAGCAAAGTAAGCAGATCTGTGTGGCCAAGAAATGGATCAC

uncharacterized protein A0A452YWY3 4.90E-09 63 deaminase

activity GO:0019239

27631

AATTCTGCGCTCGAGACCATTGCTGAGGAGGGCG

CCGCGGTCTCTAGTTGGGCCTCGGAGTACCCACCCAACATGCTCTTCTATGCGGAGTACCCTGGTTG

protein transport protein sec16

A0A1D6QV04 5.00E-09 73.2 COPII vesicle

coating GO:0048208

34161 AATTCCGGGTAGTCACGCCGGAAAGCATCTTCCCGTTCCCAAGTGGCTTCCTCCTCCGAGTGATTCCGC

CATTGCACCTTGACGAACTTGATCTTGCGACT Retrotransposon protein Q7XE96 6.70E-09 68.8

DNA integration

NA

65948 AATTCTCAGGTACATTCATGGAACAATTGAAATGGGACTGAAGTTTGTTAAAGATAGCTCCCTGCTAG

TTAGTGCTTTTTCTGATGCAGATTGGGCTGGTT retrotransposon protein Q2QY49 6.80E-09 75.8

nucleic acid binding

GO:0003676

21937 AATTCCTTACGGTCACGGTGATCTCGTGGCGTCGGCGGTACGCCTCCGTGGAAGGATCATCGGGAGCTGCAGAAACGACAGGAAGAGGGTGGTGGTGCGAG

DEAD-box ATP-dependent RNA helicase 40

A0A1E5VF87 9.20E-09 78.8 ATP binding /

helicase activity NA

41723 AATTCCTGAGGTGGCTACGGGGAAGGAATCTCTCGAGCGACGACCGCATAATGCGCCTAAAAGAAGGGTACTCTTTCATCATGTTCTCCAGTCAACTCTTT

Ubiquitin-specific protease family

A0A1D6Q0A7 9.30E-09 92.3 peptidase activity

NA

47369 AATTCCTCACCATCATCTCACTGTTATTGTTGAACGATTCGTTGAGAGTGTTCAGAAGGTTCAGGTCCT

TGGTCGAGGCTCCGATCTCGTCCTCGAGGACT

transcription factor bHLH61 A0A1E5VQ23 1.70E-08 75.8 protein

dimerization

activity

GO:0046983

34096 AATTCTCACTTCCTGCAGCACTGCCTTCCTTTTCT

GATTAGAGCTCGCTGTTTCTGCAAGTGAGTTCTCA

TCTGTCGCATTGTTATCACAACCATTTCTAT

uncharacterized protein C5WZ78 1.80E-08 75.8 NA NA


19

37576 AATTCTATGATGGATAATGGACAACCTTTTTGACATCTTTGGAAGAAACTAAGGGCCTTGCGGAATCA

TGTACGCAAACAAGTTCCGAATTTCTATCAATT

uncharacterized protein I1HU38 2.20E-08 92.6 isoprenoid

biosynthetic

process

NA

5202 AATTCCGAGTGCCAAGCACGACCCTCGCTAATGGGATACAAAGGGACTGCTTCCTGTTCATCGTGCCC

AAGTCGTACCTAGAGAAAAACAGCAAAATCTCT

uncharacterized protein A0A0A9H6B2 2.70E-08 75 NA NA

13104

AATTCTTCAGCTTCAGCGATTATTTGAGCACGTAG

CTCCTTCTCCTTCCGTTCTTTTGCCTCAAGTTCCAAAGCATTTTTCCTGAAACCATTGCGACAATT

Clathrin light chain A0A287JMT4 5.00E-08 89.7

intracellular

protein transport

NA

8288

AATTCCTTCTCCTCCCCCGCCGCGGCGAACTCGAG

CCGCCCTGGCCGCACGTCCACCGACACGCCGTGCGGCTCGGTGACCCTCGCAACGTACTCGACCGG

uncharacterized protein A0A287RQV0 6.00E-08 75.8

serine-type

endopeptidase activity

NA

30358

AATTCAGTGTATGTTATGATCTCAACTGAAGCAG

GGATCTCATTTCCGTCACCGCCTCCACCGACTGTTGGTTCAGATTGAAGATTTATCTGCTCATCATC

uncharacterized protein A0A2S3ILP0 6.10E-08 74.3 NA NA

189

AATTCACCGTCCAAACCATCTCTCATGGATCGAC

GACTGGTACGAGACCAAGACCAGCACCAGCCCCAGCGCAAGCGCGACACCCCACGGCGACGACCCCG

uncharacterized protein A0A498JNU3 6.20E-08 75.9 NA NA

30569 AATTCGTAGGAGAGGTTGAGCGGCGTCCAAACGGACTCGTTCATGACGTCGCCGAATAACCCGCTGCCGTCCTCCTTGAGCGGCGACGCGGCCACCACCGA

Anthocyanidin reductase-like

C5XDT9 1.10E-07 78.8 catalytic activity

GO:0003824

16261 AATTCCTCATGGTGTCCATCCAGTGACACGATCGTGTCGTTTGCCGAGAAGATGCTGAAATTGGAGCAG

ATGGTGGAGAAGATGACCCTGGAGGGCCTCGG uncharacterized protein NA 1.50E-07 76.7 oxidoreductase NA

56311 AATTCATCTTCGGCCTCATCTTCAGGGAAAACCAGCTCGTGGAGACGGACCCGCTCCTCAACAAGGTCGACGGAGCGACGGCGCTCCGCAGCGCCATCCCC

uncharacterized protein A0A2S3IGM9 1.90E-07 81.5 NA NA

55079 AATTCAGTCAACAGGAGCAAGTAGGTACCCTGTTTCCTCGGTCGATGAACTTCATGGCCACCAGCTCCC

GCGTCTCCTTGTTCCGCATCAGCTTGGCCACT

Serine/threonine-protein kinase SAPK5

Q7XKA8 4.00E-07 92

signal transduction of hyperosmotic

response

NA

2594 AATTCTATGGGTATTAAAATATTGAGGCGTATGGTGATTCACTTTTAGTCGTGCAGCAAGTTGCCGGTA

ATTTTCAATGCCTCGATGGTTCTCTTAATGCC

protein L0P3P1 4.00E-07 76.9 nucleic acid

binding GO:0003676

58555

AATTCCGTGAATAGAAGGGGATTTTCCACCTTCC

GTTAACGCATATGGAGCAGCAGGAGGGGAGGTTGGATCCCTCGCAGAGCGCGCAGCTGCTGCCCCGT

DNA-directed RNA

polymerase II protein A0A1D6IKB5 4.10E-07 86.4

RNA

biosynthetic process

GO:0032774

36280

AATTCTGTTATATCTGGCAAGTGGTCCTTTGCGAC

ATAGAATCAATGTTATATGGTTGTATACTGTTTGTAAAACTTAAACATTGGGATTGTGTCCTATCT

ATP sulfurylase A0A0A9GXL7 4.40E-07 72.4 NA NA

21629

AATTCAGGTGCCTCATATTTCGTTTTGGATTTGAG

GGACAATCTTGGCGGACTAGTGCAGGTATGTCAGCTATTTTGCTCTCTATCATATGATATCTCTGT

uncharacterized protein A0A287L925 5.40E-07 82.1

serine-type

peptidase activity

NA

11901

AATTCCGGATACCCATGTCGCCTCCTCTTCAGAGT

GATTATTCCATTGCACCTTCACGAATTTGATCTTGCGGCTTCGCAGTGTGCGTTCCGCTTCATCAA

protein Q01MJ7 5.40E-07 69 proteolysis GO:0006508

24343 AATTCTTGTGGTCTTCGGGGCTGCTCAAGGCTGTGGCGGCCCTGGTGATCCTGATGGCCGGCGTGCTCA

TCGGGCATGCGGCCAGCACCAACTTGTACTAC uncharacterized protein A0A368QAI2 1.00E-06 72.7

acetylglucosaminyltransferase

activity NA


20

32471 AATTCCTGATACATTTTTTATGGGCTTAGGACAATGCAAGCTCATTAAAGGCCCAGCTTGATCAGCAGA

AGGGTGTAGTTGAAAATTTGATTTCAAACACC

Probable membrane-

associated 30kDa protein Q8S0J7 1.00E-06 95.8

host-virus

interaction NA

23585 AATTCTATGCTTATTCCGTTATTCCATTGGGTGCCTTTGTGCAGTCACAAGAAGGCATCGAAGGGTCAT

ACAAATGGCCACCTTCACTTTATCTGTACGCG

uncharacterized protein A0A0A9ENA8 1.00E-06 83.3 NA NA

22278

AATTCTCGGCGCCGTACGCACCTCAACAAAACGA

CGTTGCGAAGAGCAAGAATTGCACCCTCATTGACATTGCTCAACAATACAAGACCTCTGACCGGTTT

retrotransposon protein Q10IC4 1.00E-06 64.9 zinc ion binding GO:0008270

30638

AATTCGTACCTCCTTGGAAGCATTTGGGGCATTG

AATCCGCCCCCATGTTCTGTGGGTTCCAGGCGCCCATGGATGATGAGGCCGTGACAAGCTTGCATCG

uncharacterized protein Q651N2 1.10E-06 89.5 NA NA

14606

AATTCGAGATGAAGGATTTAGGAGTAACAAAGA

AAGTTTTTGGCATGAAAATTCATAGAAGCATGACAAGTGAGAAGATGTACCTTAGTCGACAAGGTTAT

protein Q7XTM9 1.40E-06 63.6 nucleic acid

binding GO:0003676

32038

AATTCCTGCCACCAATTTCCTCAGCTCTGGACTGC

TCCTACTGTCTCTCAGGATCACTGCGAGGTTTCTAAAAAGAAGGGGATGTCTGGATATATTGTTTC

uncharacterized protein B8B5D0 1.40E-06 77.4 NA NA

38562 AATTCACGGGGATCTACGAACAGGACGCCAGCAGGCGACGCCGTCGTCAAGTCGTCCTCGTTGTCGTCG

CTGGCATCGCCATTGCCAACGGCAGCACCGAG uncharacterized protein A0A287TQI9 1.70E-06 71.9 NA NA

2218 AATTCAGACTTGCTCACCTAGGCATTTGGTGCAAAAGGATCAAGGGGATACCACTCGATCGTTAATATTTTCTCCAGTGGCACACCATAGTCTAAAAGGAC

uncharacterized protein A0A3L6PZU7 1.90E-06 80.8 NA NA

1826 AATTCTGGTCCAGTTTCGACATTCCAGGTTCATGAATATCTAAGGCCTAAGGTAATCTCTTCCATGCTAA

GATAAACAGCCAACAGTTCTTGAGGACTACA

Serine/threonine-protein phosphatase 2A 55 kDa

regulatory subunit B A0A453F0F3 4.90E-06 87.5 NA NA

1072 AATTCTATGCAGTTTCAACATCTGGACAACAATC

AATGTGGTAATGGAGTGGAAGAATCAAAGACACTGGCGACTCATAACCAAATTGGAGTTGCTCACGT

uncharacterized protein A0A1B6QNQ3 6.70E-06 74.1 G-quadruplex RNA binding

GO:0002151

5808

AATTCATGATGCGCAGTGCCAAGATAGTCAGCAA

CTTCCCTAGCAGCTTTAAGATCTGGAGAACCCTAGAATGAAGAATGAAAGAAAAACTTCATCATACT

Asparagine synthetase A0A199ULZ1 9.10E-06 95.2

asparagine biosynthetic

process /

glutamine metabolic process

NA

3235 AATTCCAATAATTCATTGAAAGCTAAGGAACAATGATTGGCAAGTGGTGGTGGAAAGATTTGAAAAGA

AATTGACTCATTGGAAAGAAAAGCTCATGTCCT

protein Q01HC3 9.20E-06 66.7 NA NA

13852 AATTCTAGAGGTTCAAGCGAAACATCCTCCCTTGAAGCTACTGGAAATAGTTCCTCAAACACCAGTGC

GCCAAAGGCAGCAAATGCATCTGACGGTTCTGG

uncharacterized protein A0A0A9DLQ5 1.20E-05 75 NA NA

13183 AATTCTTCGTCTTTTGTGTATTCTTGCAGGTATCGTGGGAGGAGCTGTACATCAAATCCTTGCTCCGAT

CCGCCCGCGGCAGCCTGATGGGACCTGCAGCC

uncharacterized protein A0A1D6M9V6 2.40E-05 90.9 NA NA

22807 AATTCAGAACTTGGTACTGCCCTCAGTGACAGTGTGTCAGATCTCAATGGAGATTTTCATAGAAATTTC

AATAATCTTTTGTATGCGAAAGGGTGCCAGCA

uncharacterized protein K3ZHB9 3.20E-05 57.6 nucleotidyltrans

ferase activity GO:0016779

5820 AATTCGAAATGAATGGTTTCTACAACCGCAGGAG uncharacterized protein A0A368SI73 3.20E-05 64.5 membrane GO:0016020


21

AAGCAGCCACGCGTGTCGCACCGTCCATCCCTAGAACACCGCCACGTACCGCCACACCCAAATGTCC

17004 AATTCGAGTTAGCACTGTCCAGGAGGAGAAGGAATCGGGGAAAGAGCTAAGTTCTCACCTCCTTCTTCT

TCCGCGCGAGGTTCCAGATCCTCCACGTCATG

Putative sucrose-phosphate synthase 3

A0A3L6DDT8 3.90E-05 67.7 NA NA

25768 AATTCGGCGGCGTTCTTCCCACCGTGGCCGTCGAATACACCAAACAACGCCTGAGAAAGTGAAGAAA

TGGATAGATTAGTGCGTTTAAAGCAAATTCTAAA

uncharacterized protein A0A3L6DF17 6.00E-05 70 metal ion binding

NA

8446 AATTCAAAGGGCTTACATTTTCCACATATTGGTATGGTACAGGCAAATTTCATGAAGATGCCATTTTCC

GATAACACCTTTGATGCTGTTTATGCTATTGA

Cycloartenol-C-24-methyltransferase 1

Q6ZIX2 8.40E-05 90.5 sterol

biosynthetic

process

GO:0016126

1249 AATTCCTCCTCGGATTTGGTGTGTCTGACGCGGTTCTTGCGCCAGATCTGAAGGAGATCGCGTCGGTGA

TCGAGGCCGGCTCGCTGTCCAAGGAGGTCCGC

26S proteasome non-ATPase regulatory subunit 3

homolog A

NA 1.10E-04 81.5 enzyme

regulator

activity

NA

41793 AATTCGAGATGTCCATGATTGGCGCCATGAGTTTGCTTCCATGATGTCGAGGGCATTCGAGATGTCCA

TGATTGGCGAGCTTACATTCTTCCTTGGCTTTC

Retrotransposon protein Q2QVL0 1.50E-04 79.2 zinc ion binding NA

36528

AATTCCTCTTTGTTTCTGATACAGGTGTGACAAGA

ACGGCGATGGGATGCTCACGGAAGATGAGGTCAAAGAGGTTAGCGACTCACCGAAAAGCACATGAT

uncharacterized protein A0A287M3Y8 1.50E-04 90.5 oxidoreductase

activity NA

35598

AATTCCCATTAGTATTATGTGAACTAGGGGATGG

GTTGCCCTGGTTCTGCTGCAGCAGTTGAGAGAACCCATGCCAGAATCTTGTGGGTGGAACCTGACTG

uncharacterized protein N1QWC7 1.60E-04 68.8 NA NA

31805

AATTCAGCCAAACTCAGTGCTGGTATTCGAGATT

GAAGTCCTCAGCGCTCAGTAACTGCGAGTGTGATTCTGTATGGGTCATTGGAATGCTATCGTGCCGA

uncharacterized protein A0A0A9UMF0 1.80E-04 69.9 NA NA

31472

AATTCCACGCTCCAGCTTCGGTTCTTCAGTTGGAC

AAGCCGCCGTTGGAGAGAGAGTCCCAGCCCCAGACGTAGCCGGCGGCGGCACAGCTCGTCACGTCG

uncharacterized protein A0A0A9QUC8 2.20E-04 66.7 NA NA

22649

AATTCCAACTGGCTCTCTTCAGGAACAGCAGCAG

GAAGAACTTGTTCGCCGATCGGGTGAGTACCCATTTTATTCTACTCCATGTATAGCAGACCATTATT

uncharacterized protein K3YZZ8 2.30E-04 85.7 NA NA

17786 AATTCGCCCAGCGCGATTTCCAGGTGCCGCTCCTTTGGCTCCATGTTGTTTCTACACAACAACAACGCTT

TCCTTATTAGTAGTAGCACATCATCATAGGT uncharacterized protein A0A368S804 2.60E-04 80 NA NA

37669 AATTCACCAACCAGGTCGATGGTTTCGTTGCTGATGGCGTCCATCGCGAGGGCGTTGGCCTCCATGGCG

GTCCACCGTACCGCAAAGGGAATCAATCTGCA uncharacterized protein A0A0A9GYP9 2.80E-04 71.4 NA NA

20139 AATTCTGGTTCTGATTTCTGCATACATATGTGCATCTGGGGAGCCCACTGATCTGCATTCACATTACCA

GAGTCCCAACCGTGAGATTCTATTACAAAAAG uncharacterized protein A0A0A9IS59 2.90E-04 78.9 NA NA

13540 AATTCATGATCTGACGATCACCACCAATAACACAAGCATAAGCACATACCCTGATTGTGTGAACTCCT

GCATAACAGCGGCAGTCTCTCGTACAAGCTGTG

Proteasome subunit alpha

type A0A1D6Q5M1 3.30E-04 80

ubiquitin-dependent

protein

catabolic process

NA

70668 AATTCTAAGTTGTCAATTCTGTGGCTTCTGTTCTCCTCAGAAGGTTCCATATAGGGACAATTACTCAAG

TTCAAGAAACTACAGAGGAGGTTACAACAATG uncharacterized protein A0A2T7CFX0 5.30E-04 76.2

nucleotidyltransferase activity

NA


22

1302 AATTCCATGTGCAGGCATCGACCATTCACCGCTTGTGGAGCACCACTCTGCTGCACCGCGCAGCAGTA

TCAGTGCGTGCACACTCTCCGACTCTCTTTGTG

BURP domain-containing

protein A0A3L6T4X2 5.50E-04 69 NA NA

1723 AATTCCTGCCTTGACATGTGCTTCATCTCCTCCAGCTCCTTCTCATACGTGCTTAACTACACGAGCCAAG

TCTAGTAAATCAGTAAACACTGACACACATG

uncharacterized protein A0A453IWN1 6.10E-04 94.4 NA NA

29809

AATTCTTAGCTGCAATGTTACCTTGTGCAGGTAGT

TAGCCGTGTAGATTTGCAAACCGTGTCAGTTTGTGTTGAGACAAAAAGGAAAGTCTGTTTCACTTC

no hit

33472

AATTCGTGGTGGACCAACCGAAACGGAAGCTTGG

AAGCGTTTTTCCGTGCGGATCCAGGCAAGAAAGATCCGTGTTCGCGACAGCATTCCCCGGGATCGGA

no hit

27483

AATTCCGACTCCTTCGACGCCTGCTTCACTGGCCC

GCTCGTGTGGGCGCTCCCGCCGCTCCGCGGAGATCATCTGGCAAGGACGCGGCGACGACCTCATCC

no hit

74818

AATTCTATATGAAAATAATCATGATAATCATGTA

CCTTTTCTTGTTTTCTTCTATAGAAATGGTACAGTAATATTTTGGAAGCTGACAAAACACAACCACC

no hit

17330 AATTCTCTATATACAGCATAATCCCATGCTATATTATGCTAAGTTAATCTTTTTTGCCAACCTTCTCTCC

CCAGCACTTAGTGCCTATGATATTCCTTAAA no hit

37541 AATTCACCTAGAATCAACGTATTTTAGTAAACGTCTGTAATTGACCCCCGACCTATCAGTCGTCTAAAACATACGCCTCATTCCCACTTTGGGAATGACCC

no hit

12720 AATTCTGCTTCTTGCTTGACCACAAAACTAAGGAACGCCCTAGCAAGTGATGCCCAAAGAGGTACTCTTGCGATCCACACGACTTCTGGCAAAGTCAGAAT

no hit

95098 AATTCTGATATGAATTGCCATTGTCAGGTTCGTTGCAAACAGTCCACATGAAAATGGAGCTTTGACATT

GCTTAAAGTTACTACAGTCCAAAGCTGGACCC no hit

34693 AATTCTTCCGCCGCCACCTGTAAGACAATTCCACGCCAGGGATTCGAGAGCTAAGCTTAAACAATTCGGGTTAACCAATACGAGGACGGTCGCTGTCAAGG

no hit

3930 AATTCTTAAAAACGACCGTGAAATGCGTAACTTATGGCTTCGGTCTCTCTCTTGAAAATCGAGATTGTG

TGGTGTTCAGTGTACCTCAGATCGAAGTTTTT

no hit

37447 AATTCAGCAGCATATATGCTCTGCTCTGCGGTTCAAATGGCCACTCACTCGAGGGTGCCGTTGTAGCTG

GAGGTCACCGTGAAGGAGGAGAAGCCGTTGTC

no hit

19532 AATTCGAACGCGATCTTAAGCATCGCTCACTAAGATCATACACAGACACATCTGCTTTTCCCCTCTACC

TGTTTTATTTGTGTTTTTTTTTTCTCGTTTCG

no hit

23454 AATTCCACATTTCTCATACCATCCCTGACAAGGAAAAGACCTCTTTTTCTCAAAGGATGATAAAAGCT

TCAAGTATGCTGTTAAGTTCAAGACACAACGCA

no hit

11636

AATTCCGCCCTGATTCGGGCGAGACCTACCTCGA

TCGACGCGGCGCGGCGGCGTCGGATCGGGATCGGGGATGCGGCGCGGGCCCCACGCTCTCGTGGCCT

no hit

39710 AATTCCAAACGTCGATACGGTGGGTAGGGTAAAC no hit


23

ATGGAGCGATCCACGACCGATACCTGCACGTTGAAGCTCTCCTCCACCTTGGTGTAGGGCGCCATGA

13144 AATTCCTATCACCACTGGTTTTATAGTCAAAAACATAAGTTCCTGTTTACCTGAAAACTGAACATGAGT

ATCTTCTTCCTTGCATATTCGGATGGATATAT no hit

35715 AATTCATCCATTCTTATTAATGAAATCATCAATTATATTTTCATATTTAGTTTCCTCCAAAATGTGATCA

CTTTCAACCACTATTGTTGCTAACGCATTGA

no hit

4848 AATTCTCAGCGACGTAGATGAGAGAGCCCTGGGGCTGCAGACGTCGAACCAGCCATTCCTGTGGGTGG

TTTCGCTCCGACGACTCCAGTATCCGTGGGCAC

no hit

39950 AATTCTTACGTGGTTGGTCTGGAACGGGCGAGGTGAATTGAATTGCGTGTGTGCGTGCAGATCAACAC

CTGCAACGGATTCTACTGCGACCAGTTCACGCC

no hit

87626 AATTCAATCAAGAAATCAGGGATCGAACTAATAGAATATCCTTCACAATATCAAGTAGCCGCCAAGAA

CTCATCAAGAATCGATCTTCACATGTAAAACAC

no hit

27151

AATTCTTGTCGTAACATGGCAAAGTAAGGTGAGT

TCTTGATCTGCAGGTGGCTACCCTTGTTGCCGTCTATGCGAACATCGGCTTCGCTTCGATCAGTTCG

no hit

18879

AATTCTGTTGTGGGCTGTTTCTCACTGTGCAAGCT

GAATTGCCATTTGTGGTTCAGGTGGAAGCGAAGAAAGCAGTTCCCAGGGACGACCATAGTATCACG

no hit

37439

AATTCAGGGTCACAAGATGATTATAAAGAAATTC

AAACCTACCTTAGCTACATTGCCCCAGCAGTCTCTTACCATTTAATGAACAATAAGGACCTTGAATA

no hit

2336

AATTCGTGAAGAAGAACCCATCGGCACTAAGAAA

CATGTCGCTTCCCTACTTGCAGTGAGTAGAGTGGATTATCTGCCCATGTTCAGTTCAAAATTGAACC

no hit

36767

AATTCTGGCCATTGAAGTGGCAGCATGACAAGCT

CCTAAATTTGACGCCTTGATATCATCCAGAGCGCAGTATCACCAAATGTCACTCCTATTTCACCGGG

no hit

17799 AATTCCTTCTTCGTCAATGTCGGCGACGCCTTGCAGGTACTACTGATTTTGGACCTTTTCATGTTTTATC

TCGATGATTAGCAAATGCACGCATGGAATCT no hit

72822 AATTCATCCTTTGGAGGAATTAAGATTTGGCGACGCACAACACTTTGAGCTTCAGTCACAAGTATATTAATCATTTACCTATCCATGCACAAAATTAATTT

no hit

27740 AATTCCCCTTTGTCTCCTTTGGTAAGTTTGGCACATGTTCATGCATCCATCTGCTAGCTATGCCAAAGGAAGGGACAATACAATCTACCAAATAGGCTGTA

no hit

14468 AATTCGCCGTACGAGAACAGCTGGTCGCCGCCAACGAGCTCGGCCAACAACACGTCGTCCATCGCCGTGGACTCACCCCCCGCCGCGGACGCCCAGACGCC

no hit

3707 AATTCGAACGCTAGCAGAGCAGCGATCCAGGGG

AGGAGGAGGTGGCACGAGTTGATTACGAACCTGG

CTGCGGATAGTGGCGTCGGCGCCCTCGACGGCGC

no hit

31217 AATTCATGGATACTCAGACCAACCATAATGACTGAAATTGTCTTATCAATAATTCTTTGTGATAGGCTG

no hit


24

GGGAATTAAGAGACCGAGAAATGGAGCTGAAG

23036 AATTCCAACTACTGCTCTCACTGAACCTTTTCTCTGTATACGTTTGTAGATTACTAGTTGGCTGGAATCA

GCGCCCAAGATATCAACTCGTGTTCCTGAAC no hit

29902 AATTCTGTGGAACAGATCTTTTGCATTTTCAAACATTCCATGTTCAACATATGACCTCAACATGAATCAT

CATGTGGCATGTCACATCCATTAGGTGAACA

no hit

5371 AATTCTTGATCAGCGTATATTTGTTTGGTTCTGACTCCCATTTCAAATCCCCCTCGGGAAGATCCTCGTG

AGGAAGGCCAAGAATCGGTACACATGGATAG

no hit

3658 AATTCAGGCTCAACACGACCACTCGGGTGGACACCGAGGATAGATTGCAGGGGATGAGATGTGTTAGC

TCCAGCTTCCACCATGGCGGCCGCTGCCTCCGG

no hit

44159 AATTCCCCATTGACACAACAAACATCAAGGTAATTATACTGGTATGTATACTAGTTTGGTGATAATCAA

TGCAACGGATCTTCATATCATGCATCTTCCTG

no hit

77255

AATTCGAGGTTGATCACCATGGCCTTTTCCCTAGC

TGCATAATTAATCTTCGCAAATTACAACACCAAAGTTTTGGGTCTTCTTGGTTTTCTCGCCAACAA

no hit

30235

AATTCTCTTATGTATTGTAGAATATACAATCCTAA

GCAATGCGGGAAAGCATAAACTCACCTCCTTTGACGGTACATATATGTAAGGAATGTTGGCTTCCT

no hit

30476

AATTCTACCGTGATGCGTCTAGTCTCCAATATGGG

AAGGTACGGCAACAGTGCCATCGCTTAATAGATCTTTTGATGTTTATGTAAGGTCCTCATGGAAAG

no hit

39357

AATTCCATTCGCTTATAGGACGGGTCTCATATCTG

ATGATATCTACAAGGTAAGACTGGAAATTTTGAATAGTATCCTAAACTGATGTTTGTATACTCTGG

no hit

16713

AATTCTATCTATAAAAGCCGCATCAGGTGAGGTC

GAGTGGCGGCGGAGAAGTCCCGAGGCGCACAAACGGGAGCCGTCGCTGCCAACGGAAACGGAAGCTT

no hit

19966 AATTCGGATCTCCACCACATTCCAATGCGTTCCCCGACCTAGAGAATAATCGGTGTTAGAAATGGAAAA

TGATTCAGAAACATACGTATGGCATCTAAGAT no hit

13763 AATTCTGCTGTTGAACAGAATAAGAAGCTTCGTGAAGAACTGGTCAGTGTTTTTTGGCTTATTTTTCCA

ATAGTTCTGGGCTCTAATGAATAAGTTACTTT no hit

35605 AATTCTATTTTGCGCTGAAATCAAAGATTCTATCAGTCTCATGAGGACGATGCGGGCCACACGGAAAAC

GGCAGCGGAATGTCCTTGGCCACGGCGGCCAG no hit

18216 AATTCGGGCCCTACTATTTCATCGCGTCACCGTATCCATTCCCAGGTTGATAACTCTGACACATGATGTT

TGCAAGGTTTTGATCGGGTTCAGTCTAAATG no hit

30183 AATTCACGCTTCCCAGGTAAGCAGATTATTTTTTGCCCGTTTGGATGGGATTACCACTTATTTCGCTCGG

TAAGCCGCTTATAAGCAGAATCAAACAGACC

no hit

35409 AATTCCTCCTCTGCAAATTATACACTACACATAAATTTCTGCAAATACATCCAACCTACAGACATTTTCC

TGACCTTTAAAGTCGAACCCGTAACCAGTCT

no hit


25

24301 AATTCCTATTGTGGCTGGTGCCACGGCCAACGGCTACACCTGATAAAAGGGGTAAGCCTGTGGCGGGT

GTGGGTGGAGGGCATGTTGTACCGCCAAAGCTG

no hit

94263 AATTCTTACCCGCAGCCGATCTTATTCTGGTGAGAGTTCGGTGGTATTTTTTTCCTGTGTATTCATTTCCA

TAGAATGTTTCCCTCACTCACCGGGATCGG

no hit

35900

AATTCTCTCAGCGTAGCTATTGCATTCAATTACAG

TAGTGCTGAGAAAATACTTACAGCTCAAGCAGCAACTGGCACAGGTTCAGGGACCCTGGAATTTCT

no hit

Supporting Information final - Ecography › sites › ecography.org › files › appendix ›...

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