Supplementary Figure 1. Supplementary Figure 2 Supplementary Figure 3 A B.
Supplementary Figure 1. RodA alignments. - Nature · supplementary figure 1. roda ... wp_013007878...
26
Supplementary Figure 1. RodA alignments. Query_RodA Bs 1 [ 1]--------SRYKKQQSPFYQGDLIFIFGVFFIISVVSIYAAGQFGQY[1]NTDWIQQIVFYLLGAVAITVLLYFDL 67 KRL33981 1 --------------------MMMLGLIGLASIYVAAVHDSSSV--NV TKQVISQVMWFVIGTAIAVIVMQFDS 51 WP_013103083 1 [ 5]SRqMSNRTSASELDWGIILALLLFMIIGLSSVYQAALHSQYGTVQMA VRTTIVQGLFWVIGAIIILFLLRFDA 78 WP_003866920 1 [ 8]-----------NFDWGLLIVVLLICVYSVIVVTSASH--TLQTGSYR --KVIIQIAAILVGLISIALICLFDY 66 WP_054095977 1 [ 1]RTvQKKRFLILNIDPFIIMMVL------VLATISIVAISSATYTDDP -TYVNKQIVWYVVGFLCMSIFLLLDY 67 WP_015798384 1 [11]TTwARLRS----YDALLWLLAAAVGVFGVVMVYSATRNQLELAGLSP HYYLDRQAIFWVLGLIVMSVVAALDL 80 WP_012897546 1 [ 2]NKkANRFTFDSRIDYGLILPAFMLILIGLYALYVAVSHDHPTQATTM ---VVQQGTWVIVGLFVALIVMHMDS 72 WP_012283736 1 ---MRWKLAR-NLDLTLVAVVLLLLTFSIVIMRSASS----NVGADP LAFARKQTIWVFVGITFVIISMFFHY 65 WP_010964563 1 [ 4]-------KLKIDKRTLKQIDFLIFLIPIVIAIFSSLNIYSAVHRNSA[1]ASTWKLQLIWCLMGIIAIYIIIKMDY 71 WP_052670071 1 [ 7]SRwQEAYAPIKHLDPVLVLSALALTGIGLVAIYSAKLQALTDQGLSP LLYISRQVLAMVVGILGMVAAAVIDY 80 WP_021295852 1 --mEMLRRNIRDLDFTVFGVLLLLAAYGTIAIDAATHSHANPYIPSH --IMVNQIVYEIVGVLALLATTVFDY 69 WP_004094495 1 [ 8]-----------NLDFVLIAVTLLIIAISLIFIGSATHINSSIEDRYW --YVQRQGAFALINIIVVFWLLHYDY 68 AHC50233 1 [13]-----------AFDWPLLAILLMFAALGLTVMHSAVG------GTDW --RFAEQSRNFIIAFFAMWTMALIPP 67 CAA74601 1 --------MARRLDWPILLAAVALSLMGSLLVYSATRNRTELNQGDQ YYFLTRHLLNTGIGLALMVATVWLGH 65 WP_011524430 1 ---MAKRVSV---DKWLFGSTLLLVFIGLIMVFSASAVMAGEKFGSP YAFFLRQLVWAVAGVGAMVVCMNIDY 67 WP_012256594 1 ----METRRWRDLDWGILVSVAVLLIIGSLALHSATL--NAVAGNGL[2]RPVFGRQIVYIVVGLIAMVAMMSFDY 69 ABF88160 1 [ 6]--------------WGLIFSVLAVCGLGIWNLASASRPPHSPV---- ---WGSQMGYLGISMVAVLVVCLVDY 58 WP_023036458 1 [ 2]N-----NIYLKKISKFSVFFVVNIL---LLFIISLSTIYSATITKSE -PFFIKEIIWFIISVFVFVGVSLVDY 66 WP_003815198 1 [13]-----------AFDWPLLLILILFTLLGLTVMHSAVG------STDW --RFAEQSRNFLIAFAAMWAMALVPP 67 WP_012893695 1 [14]RRaVTASSAVRRMDGVLLVAVAALAVIGTMLVWSSTRTWAPGST--- -GLVKKHILNLCIGTVLTGMAAMVDH 83
Transcript of Supplementary Figure 1. RodA alignments. - Nature · supplementary figure 1. roda ... wp_013007878...
Supplementary Figure 1. RodA alignments.
Query_RodA Bs 1 [ 1]--------SRYKKQQSPFYQGDLIFIFGVFFIISVVSIYAAGQFGQY[1]NTDWIQQIVFYLLGAVAITVLLYFDL 67
KRL33981 1 --------------------MMMLGLIGLASIYVAAVHDSSSV--NV TKQVISQVMWFVIGTAIAVIVMQFDS 51
WP_013103083 1 [ 5]SRqMSNRTSASELDWGIILALLLFMIIGLSSVYQAALHSQYGTVQMA VRTTIVQGLFWVIGAIIILFLLRFDA 78
WP_003866920 1 [ 8]-----------NFDWGLLIVVLLICVYSVIVVTSASH--TLQTGSYR --KVIIQIAAILVGLISIALICLFDY 66
WP_054095977 1 [ 1]RTvQKKRFLILNIDPFIIMMVL------VLATISIVAISSATYTDDP -TYVNKQIVWYVVGFLCMSIFLLLDY 67
WP_015798384 1 [11]TTwARLRS----YDALLWLLAAAVGVFGVVMVYSATRNQLELAGLSP HYYLDRQAIFWVLGLIVMSVVAALDL 80
WP_012897546 1 [ 2]NKkANRFTFDSRIDYGLILPAFMLILIGLYALYVAVSHDHPTQATTM ---VVQQGTWVIVGLFVALIVMHMDS 72
WP_012283736 1 ---MRWKLAR-NLDLTLVAVVLLLLTFSIVIMRSASS----NVGADP LAFARKQTIWVFVGITFVIISMFFHY 65
WP_010964563 1 [ 4]-------KLKIDKRTLKQIDFLIFLIPIVIAIFSSLNIYSAVHRNSA[1]ASTWKLQLIWCLMGIIAIYIIIKMDY 71
WP_052670071 1 [ 7]SRwQEAYAPIKHLDPVLVLSALALTGIGLVAIYSAKLQALTDQGLSP LLYISRQVLAMVVGILGMVAAAVIDY 80
WP_021295852 1 --mEMLRRNIRDLDFTVFGVLLLLAAYGTIAIDAATHSHANPYIPSH --IMVNQIVYEIVGVLALLATTVFDY 69
WP_004094495 1 [ 8]-----------NLDFVLIAVTLLIIAISLIFIGSATHINSSIEDRYW --YVQRQGAFALINIIVVFWLLHYDY 68
AHC50233 1 [13]-----------AFDWPLLAILLMFAALGLTVMHSAVG------GTDW --RFAEQSRNFIIAFFAMWTMALIPP 67
CAA74601 1 --------MARRLDWPILLAAVALSLMGSLLVYSATRNRTELNQGDQ YYFLTRHLLNTGIGLALMVATVWLGH 65
WP_011524430 1 ---MAKRVSV---DKWLFGSTLLLVFIGLIMVFSASAVMAGEKFGSP YAFFLRQLVWAVAGVGAMVVCMNIDY 67
WP_012256594 1 ----METRRWRDLDWGILVSVAVLLIIGSLALHSATL--NAVAGNGL[2]RPVFGRQIVYIVVGLIAMVAMMSFDY 69
ABF88160 1 [ 6]--------------WGLIFSVLAVCGLGIWNLASASRPPHSPV---- ---WGSQMGYLGISMVAVLVVCLVDY 58
WP_023036458 1 [ 2]N-----NIYLKKISKFSVFFVVNIL---LLFIISLSTIYSATITKSE -PFFIKEIIWFIISVFVFVGVSLVDY 66
WP_003815198 1 [13]-----------AFDWPLLLILILFTLLGLTVMHSAVG------STDW --RFAEQSRNFLIAFAAMWAMALVPP 67
WP_012893695 1 [14]RRaVTASSAVRRMDGVLLVAVAALAVIGTMLVWSSTRTWAPGST--- -GLVKKHILNLCIGTVLTGMAAMVDH 83
WP_010963045 1 [ 2]RL--------KKIHWLLVINVIALFCVGIVVQYSSAGGKWVP----- --FAIHQLVIFSFFFLLAIAMSFIEL 60
WP_015723471 1 [ 9]----------HHFDWVMLLMLLLVGGMALTNLYSSTYIADNGA-SS- --VFYKQLLFFSVGLVLILVVLTQEY 68
AIE75879 1 [24]EAr------------LMRTLFFAWMAMGVVVLFSASYAESLDTSGMG LSIILKQIAYLWVGLNIFNFLVRLPL 85
WP_015646126 1 -------------------------------MLFGLATLRSATYGEN EQLFTRQIVWDIAGFSLMFLVLFIKD 42
WP_006193613 1 [ 7]-----------RMDFTLIGATAAIVVMSLIVIGSATHINTAGGDHYW --FVQKQGAFVVLNVLFAIFLMNFDY 67
WP_012545997 1 [ 5]------------IDKTLIIAVTILVIIGLIAVYSSTSVLASVKAKYA[4]MIYLQKQLFTLIIGFFFIVVFIFLPV 70
WP_010943695 1 [12]------------YDLVILLMAVALTCFGVVMVYSASSVMATKKFHDG FYFLKRQGVYALLGFGVMAVAMRIDY 73
WP_012963917 1 [ 1]RL--------KGIDPVLMMALVLVQLIGFLGVFSATYKDGIS----- -PLFLKQFLYTVLGWIMLIVITFTNF 60
WP_012874018 1 [ 2]SReEITTPWWRKFDWVLLLCTLALSSFGLVMIYSATS-------DPG[4]NPLVIRQFIYLIVGLLFMSIMATVDY 72
WP_010931982 1 [ 6]----------KFDLLWLLVPLSGLIVMGLMAVYSATNGSTESV--P- --LFYKQLSWAVTGAIAASIIYFMDY 64
WP_003976192 1 [18]TRlFARDSMARRLDWPILLAAVALSLMGSLLVYSATRNRTELNQGDQ YYFLTRHLLNTGIGLALMVATVWLGH 91
WP_027868355 1 [31]GSrEKDTDSLIMYDRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDP FFFAKRDGVYLILAFILAIITLRLPM 104
WP_012546334 1 -MlKIDRRLISSFDWVTLGVVLFICIIGILTIYSATR-PPLDEGEQP[1]--FYVKQLIWLIIAIIALCVFITFDY 70
WP_011242757 1 [12]QKfAFLDAWKGMDRWLLFIPIALTLLGGLLI--------RSTQANQN FADWWQHWITGAVGVGLALAIARWRY 77
WP_013708713 1 [20]RIsSSKNSWRRQVSPSVLVSSLALISYGALVIWTASL-------SIP EASFPRQLLGIGIGLIAMVGVWRFDF 86
WP_014546566 1 MSsGRFVDKPLKIDWVFIGVTLTLMTCGVLLVYSATVNEEIAFYDTH ---WFRQIIYFLMGIAIAVGLVFVKI 70
WP_048435637 1 [ 4]AErSHLGDWWWTVDRALLAGLGVLMTAGLVFLMAGGPPVAERLGLPT FHFLNRQVMYLLPTIALILAVSFLSL 77
WP_013127264 1 [62]VNvGTALRRPLASYHLVLGLTAVLTVLGLVMVLSASSVEDISATGSP YSKFTSQLIYVGLGVVAFFGALYLRP 135
WP_011522270 1 [ 2]R-----YVSFRDFDWFLLLFVLIICTLGVIEIYSATF-----GTKFA -GAHVKQIYWVIGGVIVMFLLSLVNY 64
WP_012683246 1 -MpVTSVLRRQSIDFPLLLIALLLTAFGIAMVFSAGQIDAPSTITAG --AWKRQLSWFGLCLAATWVVTRGSV 70
WP_010942720 1 ---MIDRRLITNFDWMLLGLVLLICAVGVVNIYSASS--SYVLSGTP YY--LKQMSWVLVGLAIMLLVCSIDY 66
WP_011112575 1 [ 4]DIkKIWYYFTRYIDNFLLAGIFLLMLTGLIVLYSATG---------G[1]LTRVISQLINMIVAFVVMWTVANIPL 69
WP_003114090 1 MRrRASLLQRLHIDGILLLLLLILGATGLFVLYSASG---------K[1]WDMLMKQATSFGLGLGMMVVIAQIEP 65
WP_013066543 1 [18]----------LFLNWPLIFLLTAVAGTGFLMLYSVAGGNLETW---- ---AEPQMKRFGVGLVAMIAVGLTPI 74
WP_011744311 1 [ 6]----------NVD-FWLLVPMIGLIVFGLMAIFSATHGAGETT---- --LFYRQFAWGVIGAVVMLFVYFNDY 62
WP_002853654 1 [ 2]--------------RLDRRILTHFDYMQPILFLPIILISFFLIFEAN PFLAEKQFVYACVGLFAFMVFFFFPI 61
WP_009963846 1 --mTPLFRKFLGLNWILALNMIALIVWGVWAIYNASSFREGMNLSTV ---WRSQVQWAVLGLGVFGAAALVDY 68
WP_012851903 1 [21]HRlV----ALRRLDWKLITAVVALSVLSALLVRSATFAELAEQGRDP NGFVKRHLLNLALGLILGGVVAMLDY 90
WP_010881835 1 [10]VHqERYSF-------VFILLVALMVGVGFVTLYSGSVHYAQRFFRYP GYFLVRQGVSIGIGLVCLLFFTFVRL 76
WP_003867927 1 ---MKKKYPV---DYGILLTVLILVSIGVIMVFSASSASAEYMYNDP YYFLKRQLLWVILGFFAMVFMMNFDY 67
WP_012869947 1 [ 2]LKsPSWNEIRSTLDRPLGLITGALFSLGMVALLSAST---GVYKANY -SFPIRQALWGMVGFAGYIWVIKVGY 71
WP_012639260 1 [ 1]----RTLVRALHLDMVLFTGLLLLAGVGLVALYSATG-ESMDMLN-- -----RQLVRLAVAFTALLVMAQIPA 62
WP_013007878 1 [ 9]----------KYFDYILTLIVLTIATIGIFAIYSASFDLNSGKFSS- --FYLKQMIWLFAGILTYLLFTIINY 69
WP_010919421 1 [18]KF--------MEIDWTFCLFLCLIAGAGALMLFSIAGASWEP----- --WAAKHLIRFGLYFIMMVILAMCDL 76
WP_012415416 1 [16]SEqKNKKFTFLVPDRSLLFITAALMLFGLIFTYSSSAFES------- GNLFKRQVIYDIVGLGIAAFLSQFYL 82
WP_011564505 1 [ 9]PAsFSNLDRWRSADPVLLLVTLLLSAYGIFAVYVAGT-------SDR EAYAVNQSIGFAVGLAGAIPLALIDY 75
WP_012411137 1 [11]SSvSN----WALEARLLRWLTLIWLFVGLIMLFSASYPVADARQGDG LYYFKRQLLWVLVSLIGFNIIVNLPL 80
WP_000131719 1 [ 3]NPnKKTFWDKVHLDPTMLLILLALLVYSALVIWSASG-QDIGMME-- -----RKIGQIAMGLVIMVVMAQIPP 68
WP_041571869 1 [ 2]-------------DRPLFFLTTLAITIGVIFSYSLSAYATLFYGYNE FHFFIRQLIAGIIGIYLMWQVSRFDP 62
WP_003033307 1 [12]NSkKERVRAKLEIDISIVFIMLGLLTFGWVMVTSASMIVALDDYNNP YFYSIRQGFFAIIAIFLFLLALLVPT 85
WP_011014922 1 [29]GAwNDLLARPLTDYIMILCIVVILSCLGVVMVYSSSMTWSLREGGSV WGTAVRQGIMIVLGFFAMWVALMTRP 102
WP_012583594 1 [ 1]KR--------REIK--LILVVILLTFIGFLFIYDTTATRLIAKELSP YVFLQRQVIAFLIGLVFFAFFATTYY 64
* *
Query_RodA Bs 68 EQL--EK LSLYIFIIGILSLIILKIS[3]IAPVIKGA---KSWFRI-GR--ITI-QPSEFMKVGLIMMLASVIGKAN 135
KRL33981 52 EQL--WR VAPIAYWFGIFLLAAVLVL[3]SLFASTGA---KSWFAV-GS--LTL-QPSEVMKPAFILMLGRVVTMHN 119
WP_013103083 79 SQL--WR LAPVAYGLGVFLLVAVLIF[3]PMADATGA---KSWFVL-GP--ISF-QPSEVVKPAFILMLSRVVAEHN 146
WP_003866920 67 NTL--AK FSTFIYILNLFGLVLVLA- IGKESKGA---QSWISL-GP--VNI-QPSEFSKLALVLTLANMFSKME 130
WP_054095977 68 KIL--SQ[4]YILFGFSVLLNLLVMVPGL -GVEVNGA---QLWFRI-GG--FQF-QPSEMMKLILILILAKVLAEKK 135
WP_015798384 81 EWL--GR LGYWIYGAVLLGLVAVLS-[1]VGSSALGS---QRWFQL-GP--IQV-QPSEFAPIGVMFGIAAYLSGRD 145
WP_012897546 73 RFL--WN LTPFFYVLGLILMFLPIFF[3]ATYASTGA---KNWLAFgGR--NLF-QPSEFMKLSYILFSARIVVTFQ 141
WP_012283736 66 QTL--SR YSWYLYGLNLLILIAVLIP[1]LGVNVNGA---VRWINV-GG--FQF-QPSEFAKLLMIITFADFLSKR- 130
WP_010964563 72 DFL--KN YAEIIYGLAVVLLVINDI- FGSTRNGA---TGWIAI-GQ--RAI-QPSEFAKIALVIILAKKVDAMK 135
WP_052670071 81 RHL--RT YAAVIYGVALVLLVLVITP LGTTFGGA---QRWILI-GG--FQL-QPSELAKIAVLIAIAAVLHERK 145
WP_021295852 70 RVL--RK YRWWLYGISSFLLLVVFGL --PARNGA---HSWIDL-KV--VTV-QPSELAKIALIIWLAAFMADTD 132
WP_004094495 69 KSL--GK MAHVLYGINLVMLLAVML- IGQSALGA---QRWIQI-GP--ISL-QPSEFSKLIMIISLAHILDKRS 132
AHC50233 68 KWL--MK LALPFYVIGVVLLLGVEF- FGETSKGA---TRWLNL-GV--TRI-QPSEMMKIAVPMMLAWYFQRHE 131
CAA74601 66 RAL--RT AVPLLYGFSVFLILLVLTP LGSTINGA---HSWIKLpGG--FSL-QPSEFVKITIILGIAMLLAARV 131
WP_011524430 68 RKWknQT LIYTLLGITLALLIAVFFV[1]---RSHGA---HRWIRL-GA--ASF-QPSELAKPAIILFLAFWLEPRI 132
WP_012256594 70 RLL--SS LARPLYVSTILLLGAVLVI -GRVSEGA---QSWIAI-GE--RTF-QPAELGKLVLIIALATYWQHYA 133
ABF88160 59 RWI--QR MALPIYVGNILLLIALRF- FGHTAKGA---ESWFVI-GP--FRM-QPAEFMKIGVVLMLAKVYHDDF 122
WP_023036458 67 RKY--YK YSTAIYIFNILMLLSVLVI -GTSRLGA---KRWIDL-GP--LAL-QPSEFSKLLLIFTFSAYLINNY 130
WP_003815198 68 NTL--MK LALPFYVLGVILLLGVEF- FGETSKGA---TRWLNL-GV--TRI-QPSEMMKIAVPMMLAWYFQRHD 131
WP_012893695 84 RRL--RA YAPLVYGLSLLGLFLVITP LGSTVNGA---HSWIMVgGG--FAF-QPSEFAKLGLVLMLAMLMAQPA 149
WP_010963045 61 DFY--LK HAYFFYVAAVISLLVVNF- FGSHIMGA---TRWIRI-GS--ISL-QPSEFAKVGLILALARYFDKQS 124
WP_015723471 69 QRI--AK FGYALYAIILALLIYTLL- FVKAIAGS---QRWIDL-GF--FNL-QPSEPAKLALILVLASCYAHM- 131
AIE75879 86 QVC--LK LVPWFLIVVLLLIFLTKSG LGVEVNGA---RRWISL-GP--VLI-QPSEFMKPCLVLQSANLFGN-- 148
WP_015646126 43 RTI--RN FSIILYVFSVVLLAALLV- KGTPIGGS---KRWFRV-MG--FSF-QPSDFAKLSLIVLLPYLLEKR- 105
WP_006193613 68 KAL--QN YGRNLYIFNAVMLLLVMI- IGQTALGA---QRWIQL-GP--ITL-QPSEFSKIIMIIALAAMLEDRV 131
WP_012545997 71 TKL--KK LVFPLLIISFIMLIAVFSP LGVSAGGA---RRWLRL-WP--SVF-QPSELVKLAMVFFLAWYMSRE- 134
WP_010943695 74 RTW--RE YAVPILLGCLFLLFLVFIP[1]IGGAAKGA---SRWIRL-PG--FNF-QPSELTKIALIVYMAYSLDKKQ 139
WP_012963917 61 RML--YD MAPVIYMLNLFFLVLVPL- FGKTVYGA---KRWLDL-GP--FSL-QPSEFMKFSLLLFITYILGHT- 123
WP_012874018 73 RFL--LN WKWVIYGLVLFLLTLVFVI -GHTAYGS---TRWIDL-GP--FPL-QPSELAKLLMVLVLAGFLCEKK 136
WP_010931982 65 RVV--KD NAYFMYAAGIILLIAVLV- FGKKVAGA---TSWVRF-GM--FSF-QPSELTKMFTIIAMARFLSD-- 126
WP_003976192 92 RAL--RT AVPLLYGFSVFLILLVLTP LGSTINGA---HSWIKLpGG--FSL-QPSEFVKITIILGMAMLLAARV 157
WP_027868355 105 EFW--QR YSATMLLGSIILLMIVLV- VGSSVKGA---SRWIDL-GL--LRI-QPAELTKLSLFCYIANYLVRKG 168
WP_012546334 71 IKL--KD FWLIFYITGILLLIIVLFT -GKTAMGA---KRWINL-GF--FSF-QPSEIFKIIFIISISAFLEDKQ 134
WP_011242757 78 DNW--LK LQWWLYGVTCLSLIAVRFV -GTTALGA---ERWISI-GG--FNI-QPSEFAKPLMIVVLAAILSRET 141
WP_013708713 87 RNL--AN LSTVLIVIDIILIFSPYVP[1]LSYSAKGM---TGWIKI-PLigLTF-QPVELVKIVTIMFISALGAQYN 154
WP_014546566 71 DWL--KR AAVPSYVIALLMLVFVLIF -AGDVKGA---GRWIDL-KV--IKL-QPSEFAKIAYLITISYWLSKHP 134
WP_048435637 78 RHI--RR MALVTYGLGIVLCIAATKY -GPEIKGA---HRWIQF-GS--IGV-QPSEFVKPAFVILAAWAFAEGA 141
WP_013127264 136 TML--RR LALGSVLVSIALLIAVLIP[1]IGSKVGGA---RRWIDV-GG--FTI-QPSEIAKVALIVWGAHLLADRS 201
WP_011522270 65 HLL--LG NAHWMYLVAIVALIAVRVF -GKKYLGA---RRWIQI-GG--NHF-QPSEWVKLILILAVAKYFAEEK 128
WP_012683246 71 RLI--EW SAWPLYALSCALLVLVLFI -GTGAGTAasvKGWLSI-GG--VRIgQPAELAKLATTLMLARVLAAQR 138
WP_010942720 67 HLL--ED VAYWFYGILFLLLLVVLAF[1]--KTSMGA---TRWLHL-GF--ISV-QPSEPMKIIGIITMARILANIP 130
WP_011112575 70 QRI--MR LAFPIYVMGIILLVAVAL- FGEVQNGA---RRWLNL-GF--INI-QPSELLKIAAPLMMSWYFDKA- 132
WP_003114090 66 RFM--AR WVPLGYLVGVALLVVVDV- IGHDAKGA---TRWINIpGV--IRF-QPSEFMKLLMPMTVAWYLSKR- 129
WP_013066543 75 WFW--RN VSGVIYTIALLLLLAVEF- VGDIGMGA---QRWLDL-GP--LRL-QPSEIMKIGLVMLLAAYYDWL- 137
WP_011744311 63 RVI--RD NAYLLYLISIFLLVVVLL- FGTKIAGQ---TSWVKI-GF--FSF-QPSEIAKMATILALARFLSD-- 124
WP_002853654 62 RKF--IW IIPVAYWINIFLLLSVDI- FGVEKLGA---KRWLEI-PFthFTI-QPSEIFKPSFILMLAYLIYQNP 127
WP_009963846 69 KWV---R[1]GGWIMYLAGIAGLILVKFI -GFGEKGA---HSKIHL-GP--VDV-QPSQLAIVATIVSIAVVLGDLH 132
WP_012851903 91 RLL--RA YAPIVYGLACVGLVAVLSP LGETINGS---HSWIVLgGG--FQV-QPSEFAKVGLVVLLAMLLAEPR 156
WP_010881835 77 ASL--RK ALSPLILVAFALCVCTFF-[2]IGSTRNGA---TRWIKV-FD--INF-QPSEFVKLVLIVFLANFFDKHR 142
WP_003867927 68 TIL--KK LAGPLLIISIGLLIAVLIP[1]IGVERYNA---TRWIGV-GS--FTI-QPSEVAKYALIIYLAKYFDKHP 133
WP_012869947 72 RRI--LE MSERIYGASLLLLFLVLVM -GHTAKGA---QSWFNL-GP--VRL-QPSELGKLALGVFMARHLCRFP 135
WP_012639260 63 DNL--KR WSPWIYVLGIGLLATVLV- MGEMGKGA---QRWLDL-GF--VRF-QPSELMKIAVPMMVAWYLAD-- 124
WP_013007878 70 RFL--VK HSFLLYLLSLAILLLVLI- KGHIGMGA---QRWINI-AG--FRL-QPSEFIKVVWILFLAKQFAT-- 131
WP_010919421 77 RWW--FM AAYPIYVVGLLLLIAVEV- AGDVSLGA---QRWLSI-GG--FRF-QPSEIMKIGLVLALARYYHGLS 140
WP_012415416 83 KIQ--EK INPMFIIYGAWALLIIVL- FMPKVANV---HRWINL-GF--FNL-QPSEVAKPALMIYMAYYLSNIS 146
WP_011564505 76 RFW--QR LVKPIYLCVLLMLLAVLVV -GVAAGGA---QRWIDV-GP--VQV-QPSEFAKLMMVIVLAGYFAGRA 139
WP_012411137 81 QKI--LG VSHWFLLLFLALIFVTLI-[2]LGKKAFDA---ARWIAI-GP--IPI-QPSELIKPFLVLQSARLFGQ-- 144
WP_000131719 69 RVY--EG WAPYLYIICIILLVAVDA- FGAISKGA---QRWLDL-GI--VRF-QPSEIAKIAVPLMVARFINR-- 130
WP_041571869 63 E----KL IVKLGFTLFLLFMVLMFVM[6]MATSAGGA---KRWIRL-PF--FSL-SPVEFFKIGFVVFLAWSFSRKF 131
WP_003033307 86 KNY--EK NYNAFFFVMLIVLVAVLVP[1]IGKSVNGA---RRWIPL-LI--INI-QVAELAKLLAIIFFSGYIAENL 151
WP_011014922 103 QTI--RN LSNLILIVSIVLLLAVQIP[3]TGKEEVGS---QSWIAL-GP--IQF-QPSEIAKVAIAVWGAHYLAGKG 170
WP_012583594 65 RLW--ER VWKYVYVINLFLLVMVIF- FGKESLGA---QRWFSI-FG--FSF-QPSELSKLLLVISLSGFLTNLD 128
Query_RodA Bs 136 P[2]VRT LRDDIHLLLKIaGVAVIPVGL-ILMQDAGTAGI-CMFIVLVMVFMSGINWKLIAIIAGSG ILLISL 205
KRL33981 120 T[4]HTM ASDWQLIGKLI-AYMVPVAILlKLQNDFGTMLV-FFAILGGVILVSGISWRLLAPTFAIV AAIAGT 191
WP_013103083 147 R[4]HNT MTDWLLLGKMA-LWFLPVAALiALQNDLGTLLV-FIAIFGGVALVSGVTWRILAPVIAAA AVVGAT 218
WP_003866920 131 - -EI KTFKELLWPMA-YLGIPFVAV-MLQPDLGTGLV-FIAIFLAIVYISGIRTKVLAQLFALG IALLPI 195
WP_054095977 136 G[1]-PW SDLSSLGRICL-LFGVPFLII-LAQPDLGTSLI-LFGILLSMLLVSGLDWKWFVSGLLVI GSIAGS 202
WP_015798384 146 G --P RTWREVAVVLA-LGGVPALLV-VKQPDLGTGIV-VGIVTMVLLVMGGATGRQLLVILVAG --VLGI 208
WP_012897546 142 N[5]VLK[4]-----LIGLLI-LETMPVAIIsVFQKDFGTFLV-FIAILAGIILVSGISWKILAPAFLFV[ 1]AVAGGI 214
WP_012283736 131 Q GRL ETLKDLLPCFA-FVAVPMLPI-LKQPDLGTSLV-FIAIMLGMLAAAGANKKVLGLLVFSG[ 1]VVVIVA 198
WP_010964563 136 D GIN NVK-NFFIILI-YAAIPTALV-LIQPDMGMAMV-YFFTVLGIVFAAGLDLRVIFGGIFGI[ 1]VAVVGI 202
WP_052670071 146 G --E PGIGAIAVCLA-LTMVPMGLV-FLQPDLGTSIV-FVWLCAILFLVGGVKGRYLIGLGIAA --VTAT 208
WP_021295852 133 E[4]DYR[3]------VWVLApIVLVPFALT-FEEPALGQALV-IIAMSLTMYTVFAKRTHFAMIMLLVL[ 3]IGFSVA 204
WP_004094495 133 G -QL NTMKDVLPIFI-YVGIPFLLV-LKQPDLGTALV-FMAILFGMLFIAGIRMQLLGKIVGAG IAFLPI 198
AHC50233 132 G -AV R-IRDFLAAAA-MLAAPFGLI-VLQPDLGTALL-VFGAGFFVIYFAGLSFKLLVPVMLAG IIAIGT 196
CAA74601 132 -[4]RPH PDHRTVLQALG-LATVPMLIV-MLMPDLGSVMV-MVIIVLGILLASGASNRWIFGLLG--[ 2]TAGALA 201
WP_011524430 133 K TIT DWKHTLLPAAI-VTLMLVGII-VKQPDLGTGIA-CVAIASSMLFVAGMEMKYFGYAAL-- AAILPM 197
WP_012256594 134 D[5]LVQ[3]---------LL-IAGVPMALI-FIQPDLGTTLV-LAGIWLTMAWGGGMRLVQLITLFIAA IPLAWI 199
ABF88160 123 Q[2]APS YNLTRLWKPVL-VVFVPFALV-LVQPDLGTALM-IGLSSLTIVLFGKVRWYLVATLVA--[ 1]VFAAGI 190
WP_023036458 131 S[1]KYT -GFKAMFMSFL-HIFPIFFLI-AVEPDLGTSLV-IILIYGMLLFLNKLEWKCIATVFISI AAFIPI 197
WP_003815198 132 G -AV R-IRDFFVAAA-MLAAPFVLI-VLQPDLGTALL-VFGAGFFVIYFAGLSFKLLVPCLLAG IIAIGT 196
WP_012893695 150 A[1]TDR PRGLDVGIALV-VGAFTMGLV-MLQPDLGTTMV-LGVITAAALVVAGVRKRWIGGLALLV VGGAVA 217
WP_010963045 125 -[1]YKM MEFKRLLKALI-IIFLPVFLV-LKQPNLGTAVI-MLFIGISIIFTAIIKRSHSVICGT-- LGIFAV 189
WP_015723471 132 -[2]PGG YRLRDLIKPVF-LTALPFVLI-MLQPDLGTALI-CAIIFVSMTLFVRLRWSTLGILSG--[ 2]LACIFI 199
AIE75879 149 - WHR FPWRSRLLWLG-IFVVTLGGI-LIQPNLSTTAL-CGMGLWLIALASGLPWGYLVSTAL-- LGITTA 212
WP_015646126 106 - --- ----WFWRSFF-LTVVPAVLI-FLEPDLGTTLS-VGLIWLFAVLASNVNKKPLVILLI--[ 1]VLVFLP 163
WP_006193613 132 G -KL NTVSDILPVLG-YVALPFFLV-LKQPDLGTSLV-FIAILLGMMFVAGVNLRILAAGFAAG VAASPL 197
WP_012545997 135 -[1]YNK ESIKDFVIPIS-LMGVFQIIF-LKQPDFGAVMT-LGIITFVMLFIGGVSLRFLGLTIL-- -LAIPV 198
WP_010943695 140 D KVK FFSTGFLPYMV-LLSVVLLIL-LKQHDLGAALT-MGLVAIIMLFAAGTRPRYIIAMGM-- MALPIL 204
WP_012963917 124 - -KK SVSKESVILML-AFLIPAILT-LKQPDLGTAIS-YGIILLSLLFFKGVRLRFFFALGF-- LLLVLS 186
WP_012874018 137 R[1]--E RDLKRLIISIC-IIAPPTALV-FLQPDLGTSMV-LGAAWVSLVLFGGIPVKYLMRLFLLL IPFAVI 202
WP_010931982 127 -[2]TDI GNMMDLGKALA-IALVPAGLI-MLQPDMGTTLT-CLSFIVPMIVLAGFDLYYILLGVVPV[15]IAVLSM 209
WP_003976192 158 -[4]RPH PDHRTVLQALG-LATVPMLIV-MLMPDLGSVMV-MVIIVLGILLASGASNRWIFGLLG--[ 2]TAGALA 227
WP_027868355 169 D EVR NNLRGFLKPMG-VILVLAVLL-LAQPDLGTVVV-LFVTTLAMLFLAGAKLWQFIAIIG-- MGISAV 233
WP_012546334 135 S --P LSIKDTLKTLLiFGIIPFLLI-VKQPDLGTAIL-ILTITFIMIIYKGLRTRLMILILAIL IISVFF 200
WP_011242757 142 A[1]--R --LPGLIKAIA-IMSVPWLLI-FLQPNLGTSLI-FGAIVFGMLYWANAKPGWLLLMLSPL[16]LAIVTS 221
WP_013708713 155 G[4]--- --VRDYLKLCG-MLAVPVLAI-IALRDLGSAII-VLFAGAIVIMMSGAKKEWVLSTIALL AGLISL 220
WP_014546566 135 V -SL YKLKSFLVPLG-LFIVPFLLV-LKQPDLSTALV-FTAVTLVGFFFAGLTFTDLFLIVSPA[18]AQICLV 218
WP_048435637 142 R[4]-PG GTLAVLLLPMT-----IVPLI--LQPDFGQTML-VTMVWCAMVFVAGLHWFWVGGLGGAG --LVGV 204
WP_013127264 202 R -RG GGLKDLLLPLGpVALLMAALV-IAEPNQSTAMI-IAVTAGMLLFYAGLSSRLFLSIGVAG --ICAA 266
WP_011522270 129 S[1]EAS -GSDIVKVG-L-LVGVPFLMV-LAQPDLGTALT-YLPVAIMGFFLGGMKAKHAVVILLLV ALVIPI 194
WP_012683246 139 E -VP RSLIDLWRPLL-VVGIPWLLV-MKQPDLGTGIV-FIGICFAMLFWAGVQWQLLLMLASPG[13]AWFLIL 217
WP_010942720 131 V SGG LGLRELAFPAL-MIGAPAILI-MKQPDLGTAIL-VILIAGSMLAFVGIRLSALAAVC--- IATVPA 194
WP_011112575 133 - HIT LRWRDYVVAVL-ILLLPVLLI-ARQPDLGTALL-ILISGFYVIFLAGLSWRIIVGLAVAV AVSLPL 198
WP_003114090 130 - NLP PGLKHMVISLA-IIITPFVLI-LKQPDLGTAML-ILASGGFVLFVGGLRWRWIVGAVSAA VPIAVA 195
WP_013066543 138 -[2]EKV SSPIWVALPVA-LILMPTFLV-LTQPDLGTAVM-LTLGGGFVMFAAGVSLWYFGTIIA--[ 1]VVGL-- 202
WP_011744311 125 -[2]TDI TFTPHLLIALG-IPLFPALLI-MLQPDMGTTLT-SLSFIIPMFIMSGFDLYIVIPYLLPI[15]LAVLLF 207
WP_002853654 128 -[2]KNG YKLKQFIKLSF-YIILPFLLI-AKEPDLGSAMV-LLLVGFGVLFIMGVHYKIWLSIVI--[ 1]ISVSSP 194
WP_009963846 133 R[4]-FR[3]--LRLGVSGIL--AGVPMLMV-LKEPDLGSAAV-YGPVVVAMLLVGSIPFRYLITLFLGV MCILPV 202
WP_012851903 157 D[1]EPR[5]PGGRDIVLALA-LAGGPAVLV-LAQPDLGTTMV-FGAVVMGMLAVAGVRKRWLAGLAGAA VLAAFA 229
WP_010881835 143 E HFD TPIRSIFPPFV-VSVIFVSVV-FFQNDFSTAMF-LLFITVVMFFIAGAPLWWFLRGIMVL --APIA 207
WP_003867927 134 D YAK SFKKGVMPVLG-LAGLFFGLI-MLQPNFSTAGI-IFIVAVIILFVAGAKLSFMGALFG-- AGIGAA 198
WP_012869947 136 P[1]DLR -S---IGMALG-LSGLSLALL-MLQPDLGSALV-YCAMIGAGLWAAGIGSRYMGGLVTLG LMALPV 199
WP_012639260 125 R PLP PRFLEVVAAGL-LALIPMVLI-ARQPDLGTALL-VGAAGLLVVFLAGIRWRIIFALGALA LVALPV 191
WP_013007878 132 -[2]IEY ATFLDIFKKLI-YLIPIFLLI-FLEPDLGTALVyVYLWGIGVLYLGVKRYTVFITLII-- IIIALP 198
WP_010919421 141 -[2]SAR MSWR-LLIPAG-MIAAPVLLV-AHQPDLGTALL-IAATGLSIVVLAGLSWRIIFAGIA--[ 1]FVAAIP 206
WP_012415416 147 V SIS KSFATILPPLI-ITGVTLFLM-MLAPELGTPVL-LFCVVFLLLFVAGAKIKHLLLVLACS --VPII 211
WP_011564505 140 V[1]--- -DAGVFVRSLG-VVGAPALLV-FLQPDLGTALV-FGAVFVVMAYVGGARLWQLGALGAAG[ 1]LAAALA 204
WP_012411137 145 - WER ISWRVRFAWLG-IFGLVLLGI-LAQPNLSTTAL-CGMTIWLIALAAGLPYKYLGGTAIGG --VMLA 208
WP_000131719 131 D VCP PSLKNTGIALV-LIFMPTLLV-AAQPDLGTSIL-VALSGLFVLFLSGLSWRLIGVAVVLV AAFIPI 197
WP_041571869 132 S[5]PLV DEMKIFAPYAA-LFLLAVFLIaVLQNDLGQIFL-LGITLALMVVFAGSSFRLFFSLLMGA --LILA 202
WP_003033307 152 K KMA NFKEGILTPIT-LLGCIAILL-LMQPDFGSTVV-ISICVMGMLFVAGNKVRWYGLLLG-- TMVMMA 216
WP_011014922 171 P[4]--- --FNNHLMRFG-GVGAFMAFLiFMEGDAGMAMS-FVLVVLFMLFFAGIAMGWIAIAGVLI --IAAL 235
WP_012583594 129 -[2]KKT LGLREFILTLV-LVIIPFIVV-MIQPDLGTAIV-IFATGIFMIFLSEISVKYFLRLIL-- LGLLLL 194
*
Query_RodA Bs 206 ILLVM[ 9]VGIQDYQIKRVTSW-VSASN ETQedsNDSWQVDQAIMAIGSGGILGNGISNLKV- ---YVP-ES 277
KRL33981 192 ALYLV[14]---KQYQFARIDTW-LNPST DTS---NNAYQVWQSMKAIGSGQITGRGFNVSHV- ---TVP-VR 262
WP_013103083 219 LLALV[14]---KLYQFDRIQTW-LHPDQ DTS---ASGYQTYQSLKAIGSGQLTGNGFGDLKV- ---YVP-VR 289
WP_003866920 196 G----[ 1]KLLKPYQRNRLLSF-LNPEL DPM---GTGYHVIQSKIAIGSGMFWGKGLFDGSQT QLYYLP-EA 256
WP_054095977 203 VLLLY[ 8]VILQPHQVKRIQIF-IDPAS DPS---GAGYQSTQAKIAIGSGMLHGKGFHQGTQA QGNWIP-EP 274
WP_015798384 209 IAVVH[ 2]-LLKHYQLERLLSF-VNPQS ATQ---TYGYNLVQSKIAIGSGHIFGTGLFKGSQT NLAYVP-EQ 273
WP_012897546 215 VALVA[10]TSFAQYQVNRFIAW-LHPFE YSQ---TFSLQQARSLISVGVGGLWGKGVGVANVN ----VP-VR 284
WP_012283736 199 IYGHL[ 5]LPLKEYQIKRLIIF-LDPDL DPL---GDGYHIRQSLVAIGSGGLFGKGLFQGTQA QLNFLP-EH 267
WP_010964563 203 WNTSL --MQQYWKARLTSF-LNPEK YSS---STGLQLIQSKIAIGSGGIFGKGFLKGTQI QGGYIP-EN 264
WP_052670071 209 IAVLR[ 3]--IADYQVQRLTAF-LDASN PSL-gqGPGFQTRQSVIAIGSGQVGGKGLFQGTQT SLSYVP-EN 275
WP_021295852 205 AVMFS[15]HILKSYQADRITTW-LDPNY DVR---DAGYNIHMAQIAIGSGQLFGEGLFNGIET KGAWVP-NQ 283
WP_004094495 199 F----[ 1]FFLKDYQKKRLTVF-MDPGT DPL---GSGYHIIQSKIAIGSGMLYGKGLFSGTQS QLNFLP-EN 259
AHC50233 197 LVYYE[10]VVLHDYQKHRVCTL-LNPSS DPL---GKGFHTIQSMIAVGSGGVYGKGYMKGTQT HLDFIP-ER 270
CAA74601 202 VWQL- GILDDYQIARFAAF-ANPAL DPA---GVGYNTNQARIAIGSGGLTGSSLFEGSQT TGRFVP-EQ 264
WP_011524430 198 YWL-- LFRVAFRRKRMLAF-LDPNA DPL---GTGFHMIQSLIAVATGGITGQGLMEGKQ- KLFYLP-EP 258
WP_012256594 200 AWHYV --LDTYQQVRLSTF-YYLLT[ 1]PAAvdfNAAYNVIQALNAISSGGLTGTGLTRGLFS QGNYVP-VQ 265
ABF88160 191 VIW--[16]HHLKKHQSQRISGW-LDPEA DLR---GSGYHAAQSKIAVGSGGVSGKGWREGTQT GLRFLP-EQ 268
WP_023036458 198 SYKFL --LKAYQKDRIDTF-LNPEL DAL---GTGWNITQSKIAIGSGKIFGKGFLNNTQG KLKYLP-ES 259
WP_003815198 197 LIYYE[10]VVLHDYQKHRVCTL-LNPSS DPL---GKGFHTIQSMIAVGSGGLYGKGYMQGTQT HLDFIP-ER 270
WP_012893695 218 VWFLD -VLEPYQIARFTAF-LNPAS DPR---GVGYNSTQSLIAIGSGELFGKGLFDGGQT TGRFVP-EQ 280
WP_010963045 190 PAIW- PFLRPYHKQRILSF-LDSSV DPL---GIGYNAQQSQIAIGSGGLLGKGFVNGSQT QLGFLP-EK 252
WP_015723471 200 GWK-- FLLKDYQRKRIETF-LNPEG DPM---NHGYQIMQSKIAVGSGKVFGKGFMEGTQG HLHFLP-ER 261
AIE75879 213 VTSIS --IRDYQRARVTSF-LNPFA DPR---GDGYQLVQSLYAIASGGVLGRGFGMSQQ- KLFYLP-IQ 273
WP_015646126 164 VFFF- FGLKDYQRARILSF-LNPEE YGE---SYSYNVLQSIHAIGAGGLFGAGYMKGKAN LMGYVP-VS 226
WP_006193613 198 L----[ 1]LFLKDYQKMRLKVF-LDPSV DPL---GSGYHIIQSKIAIGSGLIFGKGLFGGTQS QLNFLP-EN 258
WP_012545997 199 LFYLA --KEPYRWKRITSF-LDPWS DPQ---GSGFQLVQSLIALGSGGLTGQGLGEGKQ- KLAFLP-EI 259
WP_010943695 205 YFLVM --NVDYRRRRILAY-LNPWE DPT---DTGFQIIQSWLAFGNGGVLGQGLGEGKQ- KMFYLP-EA 265
WP_012963917 187 PLVW- HFLKDYQRERIMAV-IDPYA DYA---GSGYQLIQSVIAVGSGGIVGKGLLKGTQS HLLFLP-EK 249
WP_012874018 203 GGRFL --LKPYQIERIAIF-LRPED NPF---GSGYNIIQATISVGSGGFWGQGFMSGSQS QLHYLR-VQ 264
WP_010931982 210 VMFFL[28]VILKPHQIKRIQIF-LDPTA DPR---GAGYNALQAKIAIASGGIFGKGFLHGTQT QLRYIP-AQ 301
WP_003976192 228 VWQL- GILDDYQIARFAAF-ANPAL DPA---GVGYNTNQARIAIGSGGLTGSGLFEGSQT TGRFVP-EQ 290
WP_027868355 234 VLLIL --AEPYRIRRVTAF-WNPWE DPF---GSGYQLTQSLMAFGRGELWGQGLGNSVQ- KLEYLP-EA 294
WP_012546334 201 LWEIL[ 1]EGLKEYQKNRLIAF-IDPNI DPK---GIGYNIMQSVITVGSGGLFGKGFLEGTQG PLKFLP-ER 265
WP_011242757 222 VGWKS[27]NLLQDYQKDRLILF-LDPNK DPL---GGGYNLIQARIAIGAGGIWGQGLNQGTQT QLRFIP-EQ 312
WP_013708713 221 VLATN[11]ALIKDYQMNRLLVF-MDPSK DTS---GAGYNLQQALIAVGSGGFFGKGIGGASQA VSGFLP-EA 295
WP_014546566 219 VFSVL[27]NMLEPHQQKRVNTF-LDPMS DPL---GDGYQVLQSITAIGSGGIGGKGFGNGSQT NLSFLP-EE 309
WP_048435637 205 AAAYE[ 2]----PHVRARITRF-MDKDS ------GDNFQTFWSQESFSSGGWLGTGPGEGIAK --RHLP-DA 261
WP_013127264 267 VFLAL --VEGYRSARLAAW-LGRSN DAL---GVSYQSNQARYSLADGGFFGVGLGNSTA- KWSYLP-NA 327
WP_011522270 195 AWMKV --LKPYQKDRLTSF-VDPEA DPQ---KAGYQVLQSLVAVGSGGLTGKGIRKGSQT QGSFLP-IP 256
WP_012683246 218 VALVL[27]DKLKPYQQKRLLVF-LDPTI DMR---GSGYHVTQSKVAIGSGGLFGQGFTQGSQK RLQFLP-ER 308
WP_010942720 195 VWL--[ 3]YYLRDYQKNRVLNF-LDPER DPL---GTGYHIIQSKIAVGSGGLFGKGFLQGTQS QLRFLP-EQ 259
WP_011112575 199 LWTFG --MHDYQRKRVMTM-LDPSQ DAL---GAGYHTIQSSIAIGSGGISGKGWLKGTQS QLDFLP-EP 260
WP_003114090 196 MWFFI --MHDYQKQRVLTF-LDPES DPL---GTGWNIIQSKAAIGSGGVFGKGWLLGTQS HLDFLP-ES 257
WP_013066543 203 -----[12]QLLHDYQFKRIDTF-LDPSA DPL---GAGYNIMQAQIALGSGGWSGRGYMQGTQS HLNFLP-EK 273
WP_011744311 208 LALWL[28]ELLKPHQLKRIQTF-LDPMS DPR---GAGYNVIQAKIAISSGGFFGKGYLEGTQT QLRFIP-AQ 299
WP_002853654 195 IIYT- HLLKPYQKQRIHDF-ISEKP --------SYQVAQSMIAIGNGGLTGKSQDEATQT HFKFLP-IS 252
WP_009963846 203 AYFFG --LKPYQKKRVEVF-VNMLT[ 4]DTL---GDAYMADKVKIAVGSAGFEGKGPLSSKVD[5]RTFFTPtEA 274
WP_012851903 230 VWFFG -LLKPYQIARFTAF-IDPEA DPR---GAGYNAQQARIAVGSGGLTGKGLFEGEQT GGHFVP-EQ 292
WP_010881835 208 VLMI- -VTSTNRLRRVLSF-LYPDR DPL---GAGYQVNAALEALMDGGLWGRGIGNGVR- KIASVP-EV 268
WP_003867927 199 VVV-- FSSFEYIRERVFTF-LNPWQ DIQ---KSGYQIVQSLYALGSGGLFGVGLGGSRQ- KLMYLP-MP 259
WP_012869947 200 AW-GF --LKPYQRMRLLVF-IDPKV DPL---GAGYNVIQSRIAVGSGGLFGKGFMGGTQG RLHFLP-EP 260
WP_012639260 192 LWHF- --MRPYQRQRVLTL-LNPES DPL---GAGYHIIQSKIAIGSGGLYGKGWLNGTQS QLEFLP-ER 252
WP_013007878 199 VGW-- NHLKDYQKKRVITF-LNPEK DPF---GAGYHVIQSKIAIGSGGLKGKGFLKGTQS HLKFLP-ER 260
WP_010919421 207 PFVM- FVLHDYQRHRVMTF-LNPEA DPS---GKGYQIVQSKIALGSGGLLGKGFGLGSQS QLNFLP-EK 269
WP_012415416 212 LHQL- -IFYSYRLKRLFSF-LDPEE TAG---TTGYQLFQSFLAIGSGGWFGKGLGNSEL- KLQYLP-AA 272
WP_011564505 205 LRFRL --LEEYQIARLTAF-LDPES AGE----IGYQVTQSKLAIGSGGLTGKGLDATTLA NLGFLP-ED 265
WP_012411137 209 ILSI- -SIKEYQRKRVMSF-LNPWA DAT---GDGYQLVQSLLAVGSGRTWGAGFGLSQQ- KLFYLP-IQ 269
WP_000131719 198 LWFFL --MHDYQRQRVMML-LDPES DPL---GAGYHIIQSKIAIGSGGLRGKGWLHGTQS QLEFLP-ER 259
WP_041571869 203 IAVI- -ISSDHQILRIKLWwANAQN[16]ENL---PEPYQIHHSLNSIQNGGFFGEGLGNGLI- KLGFLS-EV 280
WP_003033307 217 AMLVI --ISPYRMHRITGF-LHPWE NAN---GSGYQLVQALIGFGRGGWFGDGLGNGIQ- KQFFLP-EA 277
WP_011014922 236 AVLAL[ 2]----GFRSSRFEVY-FDALF[ 4]DVR---GIAFQSYQGFLSLADGSGLGVGLGQSRA- KWFYLP-EA 300
WP_012583594 195 PFFW- LILKPYQQQRIITF-LDPMK DPL---GSGYQVIQSLIAIGSGGIWGKGWFQGTQT HLNLIP-EQ 257
*
Query_RodA Bs 278 TTDFIFSIIGESFGFIGCAIVVIMFFFLIYRLVVLIDK-ihPFNRFASFFCVG-YTALIVIHTFQNIGMNIGIMPVTGIP 355
KRL33981 263 ESDMIFSVIGENFGFIGCAVVILLYFLLIYQMIRVTFD---TKNEFYAYISTG-VIMMILFHVFENIGMSIGLLPMTGIP 338
WP_013103083 290 ESDMIFSVIGESFGFIGGALLIALYFGLIYRLIRATFK---AQNAFYAYIATG-VVMMVLFHVFENIGMSIGLLPLTGIP 365
WP_003866920 257 WTDFIFSVVGEELGFIGASILIVLYAIMLYRAWKIAYN---AKDKYGMLVAVG-IIAMFTFHIFENIGMTIGIMPITGIP 332
WP_054095977 275 HNDFIFAVYAEEFGFIGASILICAYLLLIYRMLVVALR---SDHLFGTYIVAG-VMGMFVFQIYQNIGMTIGLMPITGIP 350
WP_015798384 274 QTDFIFTAVGEQLGFIGAGSLVLVYGIMLARSYRVLRS---AADRVSMLLVAG-AIAWIGFSVFQNIGMTIGIMPITGIP 349
WP_012897546 285 ESDMIFTVIAEDFGFVGSAFLIFLYFMLIYRMIRVTFK---SNNQFYTYISTG-IIMMILFHVFENIGAAIGVVPLTGIP 360
WP_012283736 268 HTDFVFSVVGEELGFIGAVALLALFFVIILRGLRIALD---ARDTFGSLIVTG-IVSMWLFHVLVNVGMTTGIMPVTGIP 343
WP_010964563 265 YTDFIFSVVGEEWGLIGAIVLMLLYIILIYRIIKMSK---nSKDAFGKIFCVG-MASTFLFSVYQNMGMTIGIAPISGLT 340
WP_052670071 276 HTDFIFTVIGEEFGFLGAMIVLGLFLVLIWRGLRIAVI---AKDLFGTLLAVG-VVAMLALQVFVNVGMTVGIMPVTGIP 351
WP_021295852 284 WTDYIFSAIGEEFGFVGAAVLVLLFLILMYRLVKIAAT---TDDPFGMYMAIG-VIGMFSFQVFENIGMDMYLSPSTGIT 359
WP_004094495 260 HTDFIFAVIGEEVGFIGSVLVLALYMLLLYRGVKIAGA---ARDNFGTLLAAG-IVSMLAFHLLVNVGMTAGIMPVTGIP 335
AHC50233 271 TTDFIFAVYAEEFGLYGGIAILVLYGLMMARGLTIASR---ASSQFGRLLSGA-LTMMLFIYVFVNVGMVTGILPVVGVP 346
CAA74601 265 QTDFVFTVAGEELGFLGAGLIIALLGVVLWRGCRIARS---TPDLYGTVVAAG-IVAWFAFQTFENVGMTLGIMPVTGLP 340
WP_011524430 259 HTDFIFAVTSEELGLVGSVTVVLLFAIFLYRGIRAAVM---TEDTFGRLLATG-ITAMVVVQAFFNVSVVLGLLPTKGIP 334
WP_012256594 266 HTDFIFAVIGEELGFIGGVVLIIFQAVVLWQTLSIAGK---ARDQFGRLIALG-IFGMLFSHTVINIGMNMSLLPVTGLP 341
ABF88160 269 HTDFIFSVWAEEHGFVMCIVLLVLYGAIFIFGLGVGFN---ARDRFGAFVAVG-VVAMLFWQVFENIGMVIGLLPVTGIT 344
WP_023036458 260 HTDFIGSVFLEERGFLGGSMLLLIYIVLLAQIIYIADT---TEDKFGKYVCYG-VATIFFFHIFVNMGMIMGIMPVTGLP 335
WP_003815198 271 TTDFIFAVYAEEFGLYGGVALLVLYGLFMARGLAIASR---ASSQFGRLLAGA-ITMMMFIYVFVNVGMVTGILPVVGVP 346
WP_012893695 281 HTDFIFTVAGEEFGFLGSVTVVALLGVILLRGMRIARQ---CDDRFGTLTAGV-IVCWLAFQSFVNIGMTIGIMPITGLP 356
WP_010963045 253 RTDFAFAVLSEEWGFLGSMALILLYTSLLGIIFSIAYR---SKNYFSKSISIG-IFAFFSAHFFINIGMTMGLLPVIGDP 328
WP_015723471 262 HTDFAFSVWAEEWGFAGSLFFLSCYFFMLIWGINIAMS---AKDKFGSILAFG-CIMLIFWQAVINLFMIMGFLPVVGVP 337
AIE75879 274 TTDFIFAVFAEEFGLIGCIVFLLFLGLFTTMGLRVAMR---CRHRVKRLIGLG-VVIFLVGQSLLNIGVASGALPTTGLP 349
WP_015646126 227 YTDFIVSVIGEEFGFIGIVFLLSLFGLLFFEVSRWILN---VKDEYWEILMVS-ACGLIWFHVFENVSMNLGLLPVTGVP 302
WP_006193613 259 HTDFIFAVVGEELGFVGAAALLLLYLVVLWRGVKIARD---ASDTFGRLLAVG-ITSMLAFHVLVNVGMTTGIMPVTGIP 334
WP_012545997 260 HTDFIFAHIGEEMGFIGVCVVVILFFFICMRGLNIAAK---QIDPFCYFLASG-ITIMISIQALINFAVVTGLAPTKGLP 335
WP_010943695 266 HTDFILSVTGEELGLIGVTVIAAMFLMLVLRGVRVALM---AQEPFGRFLAFG-IATLLGIQSFVNMAVVTGLLPTKGLA 341
WP_012963917 250 HTDFIFSVIAEEGGFVLSLLLLSLYFLLIYRLITYGMR---IYDGNQRLFLGG-AVSLLLFQVFVNLMMTMGLMPVVGIP 325
WP_012874018 265 HTDFIASVIGEEFGFIGMMALLVVYGLLLWRIIRIASK---ARDKYGELIAVG-VAAIILFQVFVNIGMNIQLMPVTGIP 340
WP_010931982 302 WTDFIFCVIAEELGFLGSTLLLLLFAALVLRLVWMVGA---IKNRFVELLLAG-YASLLLTHVVINIGMTIGVMPVIGVP 377
WP_003976192 291 QTDFVFTVAGEELGFLGAGLIIALLGVVLWRGCRIARS---TPDLYGTVVAAG-IVAWFAFQTFENVGMTLGIMPVTGLP 366
WP_027868355 295 HTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKaleIDHRFSGFLACS-IGIWFSFQALVNVGAAAGMLPTKGLT 373
WP_012546334 266 HTDFIFPIFAEEWGFIGCLILLSLYFTFFIRCFQTSII---AKNNFGKLLALG-FTSIFILYFFINIGMTLGIMPVVGIP 341
WP_011242757 313 HTDFIFSAVGEELGFVGSIAVILLFWLVCWRLIAIATS---ARDNFGSLLAIG-VLSMLVFQIVINIAMTIGLGPVTGIP 388
WP_013708713 296 QTDFVFALLSEEFGFIGAFLLLVLFAWLILSAIRVAVK---TDNLFMKLVCVG-IVGMWTFQVFENVGMCIGLMPITGIP 371
WP_014546566 310 HTDFIFSVLGEQFGFVGCAVILVLFTLFLWRASSICKT---NDDPFVTLVTMG-AATIFLFHITVNIAMTIGLMPVTGLP 385
WP_048435637 262 HTDFIFSVAGEEFGVIICIGIVLLFAFIVMRGLVLARR---SDDIFCRLAITG-LTALFGLQACINMAVNVRLMPAKGMT 337
WP_013127264 328 HNDFIFAIIGEELGYLGAGVVILMFGLLTWVGLRIACR---VADPFLQLMAAT-ITTLIALQAIINMGYVVGLLPVTGIQ 403
WP_011522270 257 QTDFIFAAFSEEHGFVGAIFLLLLYFVVLMRLIHDAQT---APDRAGTFIVMG-VVAVLAFHILVNVGMVVGFMPVTGIP 332
WP_012683246 309 HTDFIFSVVGEELGFLGVSIALALFLALFLRSTRVASR---ANDAFPSLVAFG-FVAAWFVHVMVNVGMTLNLMPVTGIP 384
WP_010942720 260 HTDFAFSVFAEEWGFVGSLVVLLLYAVLTLWGLQIARR---CNDRFGSLVAVG-VTAMLFWHIIINIGMVIGLFPVVGVP 335
WP_011112575 261 STDFIFSVFSEEFGLIGNSLLLSLYLIVIGRCLVITAR---APTRFTRLVAGS-ITLTFFTYVFVNMGMVSGILPVVGIP 336
WP_003114090 258 HTDFIIAVLGEEFGLVGVCLLLVLYLLLISRGLVITAQ---AQTLYGKLLAGG-ITMTFFVYVFVNIGMVSGLLPVVGVP 333
WP_013066543 274 HTDFIFTTLAEEFGFIGAFGLLSLYVGIIAFATYTALS---TKDRYASLVSLG-VAGTFFFFFAINMGMVMGLMPVVGVP 349
WP_011744311 300 WTDFIFCVIAEELGFVGSFVLLLLFLVLILRLLWIISS---IKNKFVELTLAG-FVSLLLIHVIINIGMTIGLIPVIGVP 375
WP_002853654 253 TSDFIFAYMIERFGFIGGLTLIIFYILLIFHLLSLNYK---LKDDYFARVAINcVALFIFIYAAVNISMTIGFAPVVGIP 329
WP_009963846 275 INDFIYSVIVEEFGFRGGLLQIVVMALLLLQCIFVSFY---ARDNLGRLIVVG-IVGMLFAHSMQNMGMNILMMPITGLP 350
WP_012851903 293 QTDFIFTVAGEELGFIGSALLIGLLGVVLWRGLRIATA---AADPFGALVAAG-VVCWLGFQTFENIGMTLGIMPITGLP 368
WP_010881835 269 YSDFIFVVIGEEMGFIGVCLYLMLLFAFTLTGISIALR---CANRFNTFLAFG-ASAAIVLQSILNVAVVVRLVPATGIP 344
WP_003867927 260 HNDFIFSIIGEELGLVGTVTILLMFLYLILRGLRVAAK---APDMFGCLLATG-ITSLIGVQTLINVAVATSSMPPTGVS 335
WP_012869947 261 HTDFIFSVFAEEFGFVGGVAAVLLFVALIWRIFQIAFE---ARDLRAKVLCSM-LGAWMFFQTFESIAMSMGLAPVTGLP 336
WP_012639260 253 STDFIFAVYAEEFGLLGGLLLLSLYLLVILRGLYISVK---AQDAYARLLGGS-LSLTFFVYLFVNVGMVSGLLPVVGVP 328
WP_013007878 261 HTDFIFSLICEEFGFIGGATLLSLFLLLLMRIIYIAIL---TKEPSGKLIALL-TAALIFFQTYVNAAMTMGIMPVVGIP 336
WP_010919421 270 QTDFIFATLAEEFGFVGCFAVLFLYGAVIFMALRIASI---SHSHFGRLAAGG-TISTFAVYVLINGAMVMGMAPVVGVP 345
WP_012415416 273 HTDFIFAIISEEIGLFGSLIIIAFFVWLLVCGVNIARR---SKNTFNSMLALG-LTLTITLQAFFNMGVATGLLPTKGLP 348
WP_011564505 266 HTDFIFANLAERVGFAGSFLLLLLFFLLIWRILHIATI---SRDRFGVLISVG-VATIFLFHVFVNVGMTMGIMPVTGIP 341
WP_012411137 270 DTDFIFAVFAEEFGFVGSMVLLALLATFATLGLIVALK---AKNTVHRLVAIG-VTIVMVGQSLLHIGVATGSLPTTGLP 345
WP_000131719 260 HTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAAR---AQTTFGRVMAGG-LMLILFVYVFVNIGMVSGILPVVGVP 335
WP_041571869 281 HTDIVLAGIAEETGFVGLFFCTLLFCAIIYRIFKIANR---SENNVFYLFCVG-AGILLSFSFMINAYGISGITPIKGIA 356
WP_003033307 278 HTDFITSVIAEELGVVGLMVLLAVYLFIVFRAMSIAKMafeLNRYYQAFLAYG-VGFWIAFQVFVNIGVNTGLLPTKGLT 356
WP_011014922 301 KNDFIFAIIGEELGLWGGALVIALFAGLLYFGLRTAKK---SHDPFLGLMAAT-LTASVVSQAFINIGYVVGLLPVTGIQ 376
WP_012583594 258 HTDFIFSAIGEEFGFIGSLFVVFLYYLLFRYTWEIWGS---LKDKFGKYIVGG-ILFCWFFQTFINLCMVSGISPVVGIP 333
*
Query_RodA Bs 356 LLFVSYGGSSTLSTLIGFGIVYNASVQLTKYRSYL[ 3] 393
KRL33981 339 LPFISQGGSALIANMAGIGLMMSMRYHYKSYMFSR[ 5] 378
WP_013103083 366 LPFISQGGSSLLGNLIGVGLILTIGYQQQNATFTE[ 6] 406
WP_003866920 333 LPFMSYGGSAMVANMMAIGLLENISMRRQKINF-- 365
WP_054095977 351 LPLISYGGSSLLTQMLAIGFVVNVGMRTQTDALSF 385
WP_015798384 350 LPFVSYGGSAMLGFSSAVGIVLSAGSRRLRRAS-- 382
WP_012897546 361 LPFISQGGSALMANIIGLGLVLSMKYNQLPEFVKE[ 19] 414
WP_012283736 344 LPFVSYGGSAMLTNLVCLGLLLNVHWRRQSIMF-- 376
WP_010964563 341 LPFMSYGGSSMFTAFIAIGIILSIGVRKNKINF-- 373
WP_052670071 352 LPFVSYGGTSLIVWFAIVGLLLNVHMRRF------ 380
WP_021295852 360 LPFISYGGTSLVIDYVAVGLVLSVGVRRRKLRFD- 393
WP_004094495 336 LPLMSYGISALTTNMVSLGILLNIYMRRQKIMF-- 368
AHC50233 347 LPFMSYGGTALFTMGIAFGIMMSISRHRSVKS--- 378
CAA74601 341 LPFVSYGGSSMFAVWIAVGLLQSITVQRP----MS[ 1] 372
WP_011524430 335 LPFVSYGGSSLFMTLASVGVLLNITQQAE------ 363
WP_012256594 342 LPFVSAGGSFMVTTLIAIGLLQSISLRHRHIAF-- 374
ABF88160 345 LPLMSYGGSSLLSVMLSIGLLVNISMRRH----MF 375
WP_023036458 336 LLLMSYGGSSLVFSFLILGVVQSVKIHRGNK---- 366
WP_003815198 347 LPFMSYGGTALLTMGIACGILMSISRRRPAPA--- 378
WP_012893695 357 LPFVSYGGTATFANMIAIGLLQAIHIREQSF---- 387
WP_010963045 329 LPFLSYGGSTTAASLICIGLLLAIKADEQ-QNACI[ 5] 367
WP_015723471 338 LPLFSYGGSSLLTNMVAIGILMNVRMRSF----PT[ 6] 374
AIE75879 350 LPFFSYGGSSCLSSLVLAGLLVRVARESNEAEVIP[ 9] 393
WP_015646126 303 LPFISYGGTSTLVFSILVGLILKGIALAR-----V[ 4] 336
WP_006193613 335 LPLMSYGVSSLTTNLIAITILLNIHMRKAKFVF-- 367
WP_012545997 336 LPFISYGGSSLVVNLIAVGVLLNLSRFDYKADFTE[ 22] 392
WP_010943695 342 LPFISYGGSSLIVTLFAVGILLNISTRLKGAP--- 373
WP_012963917 326 LPFVSFGGSSVLTFSMLLGVCFSIVREYRLKDIHF[ 4] 364
WP_012874018 341 LPFISYGGSSLVTLLTSEGILQSIILRHKKVNL-- 373
WP_010931982 378 LPFISYGGSSLVANMMMVGLAMNFSKNRR----HI[ 2] 410
WP_003976192 367 LPFVSYGGSSMFAVWIAVGLLQSITVQRP----MS[ 1] 398
WP_027868355 374 LPLISYGGSSLLIMSTAIMMLLRIDYETRLEKAQA[ 6] 414
WP_012546334 342 LPFMSYGGTTLLANFIGIALVINVRMRRFELFYP- 375
WP_011242757 389 LHWLSYGRSALLANFIAIGIVESVWRFRQISRY-- 421
WP_013708713 372 LPFISFGSSSMIIQCMAVGLVQSIWRHHVSR---- 402
WP_014546566 386 LPFLSYGGSFALVCLFLVGLLMCLRYQGNK----- 415
WP_048435637 338 LPFISYGGSSLISLALGMGFLIAITRRRPRTTLLN[ 7] 379
WP_013127264 404 LPLLSAGGNSVILVLFMLGLLAGAARHEPEAIAAL[101] 539
WP_011522270 333 LPLMSYGGSSVLFMFLALGIVMNVRMRRFVN---- 363
WP_012683246 385 LPFFSYGPSFLLVSWVAVAVLLRISAEGRGQPDAI[ 2] 421
WP_010942720 336 LPLFSYGGTSMITSMTGIGILLNISMRRFMF---- 366
WP_011112575 337 LPLISYGGTSMVTILLGFGILMSIHTHPKLVKT-- 369
WP_003114090 334 LPFISYGGTSLVTLLSGFGVLMSIHTHRKWIAQV- 367
WP_013066543 350 LPMVSYGGTAMMILLAAFGLVQSAHVHRP----R- 379
WP_011744311 376 LPFVSYGGSSLLGNMIMVALALNFVHNKR----SI[ 2] 408
WP_002853654 330 LPFFSYGGSSFTIFMIFFGILQHLITFRY-----F[ 7] 366
WP_009963846 351 LPFTSYGGTFLIVCMFLMGMVQSVWIHRNISPVKK[ 13] 398
WP_012851903 369 LPLVSYGGSATFANMIALGLLQAVHLRARPFPA-- 401
WP_010881835 345 LPFFSSGGSSIVVTLSLCGLIINVSGDEKIRRE--[ 7] 384
WP_003867927 336 LPFISYGGTSTVIMMAGVGILLNISRYANLDRS-- 368
WP_012869947 337 LPLFSYGGSSLVAEMLALGLVQSVAVESRRERFDL[ 3] 374
WP_012639260 329 LPLVSYGGTSMVTLLAAFGILMSIHSHRRLLAR-- 361
WP_013007878 337 MPFVSYGGSSLITLCSLCGIVNSIALRRY----DI[ 2] 369
WP_010919421 346 MPMLSYGGTVMLTVMIGFGLIQAVRVHRYTEVTSG[ 5] 385
WP_012415416 349 LPFFSYGGSSFLITMAMMGMLLNISAVENKAD---[ 3] 383
WP_011564505 342 LPFISYGRSSLVVSVLSLGLLQSVAMRSRAEVAKH[ 3] 379
WP_012411137 346 LPMFSYGGNSMIASLIAAGLLIRVARESNEAEVVP[ 20] 400
WP_000131719 336 LPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV 370
WP_041571869 357 VPFYSYGGSSLVANCLAIGMILSISKKAKM----- 386
WP_003033307 357 LPLISYGGSSLLIMCYTLGVLVRVDFENKLLADTI[ 10] 401
WP_011014922 377 LPMISAGGTSAIITLASMGLLISCARHEPETVSAM[139] 550
WP_012583594 334 LPFISFARTSLITNYAMLGLIVNIY-------VRG[ 5] 366
Supplementary Table 1. Candidates for the missing GTase genea.
Gene BSU codea Operon structureb σM c MOEd Fi in
Δ4 / WTe MOE-growth assaysf
Known or possible function
divIC BSU00620 + + Essential Unknown (cell division) tilS BSU00670 tilS hprT ftsH coaX yacC + + Essential tRNA modification hprT BSU00680 tilS hprT ftsH coaX yacC + Essential Nucleotide metabolism ftsH BSU00690 tilS hprT ftsH coaX yacC + Protease coaX BSU00700 tilS hprT ftsH coaX yacC + 2.2 Coenzyme A synthesis yacC BSU00710 tilS hprT ftsH coaX yacC + 2.8 Tested Unknown disA BSU00880 disA yacL ispDF + + Diadenylate cyclase yacL BSU00890 disA yacL ispDF + Tested Unknown ispD BSU00900 disA yacL ispDF + Essential Lipid II synthesis ispF BSU00910 disA yacL ispDF + Essential Lipid II synthesis yceC BSU02890 yceCDEFGH + + Tested Unknown yceD BSU02900 yceCDEFGH + + Tested Unknown yceE BSU02910 yceCDEFGH + Tested Unknown yceF BSU02920 yceCDEFGH + Tested Unknown yceG BSU02930 yceCDEFGH + 1.4 Tested Unknown yceH BSU02940 yceCDEFGH + Tested Unknown ycgQ BSU03240 ycgQ ycgR + + 5.7 Tested Unknown ycgR BSU03250 ycgQ ycgR + + 13.6 Tested Unknown amj BSU04230 + + 12.2 Tested Lipid II flipping ddl BSU04560 ddl murF + Essential Lipid II synthesis murF BSU04570 ddl murF + Essential Lipid II synthesis ydcA BSU04610 + Tested Unknown ydfM BSU05470 20.5 Tested Unknown yebC BSU06380 + + 23.9 Tested Unknown ltaSA BSU07260 + + 9.7 Tested Lipoteichoic acid synthesis yfiC BSU08220 + Tested Unknown yfiN BSU08330 21.5 Tested Unknown
Gene BSU codea Operon structureb σM c MOEd Fi in
Δ4 / WTe MOE-growth assaysf
Known or possible function
yhbI BSU08990 69.9 Tested Unknown yhdK BSU09500 sigM yhdL yhdK + + 4.7 σM regulation yhdL BSU09510 sigM yhdL yhdK + + 5.1 Essential σM regulation sigM BSU09520 sigM yhdL yhdK + + 29.6 Tested Transcriptional regulation yjbC BSU11490 yjbC spx + Tested Unknown spx BSU11500 yjbC spx + Transcriptional regulation abh BSU14480 + + 1.7 Tested Transcriptional regulation ylaA BSU14710 + 1.9 Tested Unknown ylzH BSU15069 13.1 Tested Unknown murG BSU15220 murGB divIB ylxWX sbp + + 14.3 Essential Lipid II synthesis murB BSU15230 murGB divIB ylxWX sbp + + 12.1 Essential Lipid II synthesis divIB BSU15240 murGB divIB ylxWX sbp + + 5.9 Essential Unknown (cell division) ylxW BSU15250 murGB divIB ylxWX sbp + + 4.5 Tested Unknown ylxX BSU15260 murGB divIB ylxWX sbp + + 3.4 Tested Unknown sbp BSU15270 murGB divIB ylxWX sbp + + Tested Unknown pbpX BSU16950 + + 2.3 PG turnover yngC BSU18190 + 2.9 Tested Unknown metA BSU21910 metA ugtP + -1.8 Methionine synthesis ugtP BSU21920 metA ugtP + 1.5 Glucolipid synthesis recU BSU22310 recU ponA + + 5.9 DNA recombination ponA BSU22320 recU ponA + + -186.5 PG synthesis ypbG BSU22980 + + 5.0 Tested Unknown ypuD BSU23300 + + 5.1 Tested Unknown ypuA BSU23370 + 11.0 Tested Unknown yqjL BSU23830 + + 4.0 Tested Unknown yqiG BSU24210 + Tested Unknown yrhL BSU27140 + 1.5 Tested Unknown yrhJ BSU27160 yrhH fatR yrhJ + + 2.6 Tested Unknown fatR BSU27170 yrhH fatR yrhJ + + 1.5 Tested Transcriptional regulation
Gene BSU codea Operon structureb σM c MOEd Fi in
Δ4 / WTe MOE-growth assaysf
Known or possible function
yrhH BSU27180 yrhH fatR yrhJ + + 15.9 Tested Unknown secDF BSU27650 + Protein secretion yrzF BSU27785 + 2.1 Tested Unknown minD BSU27990 maf radC mreBCD minCD + 3.5 Tested Cell division regulation minC BSU28000 maf radC mreBCD minCD + + 3.7 Tested Cell division regulation mreD BSU28010 maf radC mreBCD minCD + + 5.7 Essential Unknown (cell shape) mreC BSU28020 maf radC mreBCD minCD + + 6.2 Essential Unknown (cell shape) mreB BSU28030 maf radC mreBCD minCD + + 5.0 Cytoskeleton radC BSU28040 maf radC mreBCD minCD + 7.6 DNA repair maf BSU28050 maf radC mreBCD minCD + + 6.8 Unknown ytpB BSU30500 ytpA ytpB + Unknown ytpA BSU30510 ytpA ytpB + + Tested Unknown yvrD BSU33190 + Tested Unknown tagT BSU35840 + + 2.3 Tested Teichoic acid synthesis rapD BSU36380 + -1.8 Competence regulation bcrC BSU36530 + + 5.7 Tested Lipid II recycling ywnJ BSU36540 + + 1.3 Tested Unknown ywzG BSU38018 12.7 Tested Unknown rodA BSU38120 + + 4.7 Essential Unknown (cell shape) ywbO BSU38250 efeB ywbO + -1.4 Tested Unknown efeB BSU38260 efeB ywbO + Iron uptake ywbG BSU38330 16.5 Tested Unknown ywaC BSU38480 + + 5.3 Tested (p)ppGpp synthase dltA BSU38500 dltABCDE + 3.6 Tested
Teichoic acid synthesis
dltB BSU38510 dltABCDE + 6.2 Tested
Teichoic acid synthesis dltC BSU38520 dltABCDE + 7.7 Teichoic acid synthesis dltD BSU38530 dltABCDE + 7.7 Teichoic acid synthesis dltE BSU38540 dltABCDE + + 7.3 Teichoic acid synthesis yxeF BSU39570 24.4 Tested Unknown
a Genes and BSU code organized by increasing BSU number.
b A few σM-dependent genes belong to same operon 10 26,27, as indicated.
c Known σM-dependent genes are labelled “+” 10 26.
d Genes reported to belong to the MOE stimulon are labelled “+”10.
e Fold increase (Fi) in transcription (RNA-Seq data) of genes in strain Δ4 (AG157) relative to the wild type (168CA), in mid-exponential cells
grown in nutrient broth at 37°C. “-“ indicates down regulation.
f Around fifty gene deletion strains were tested by MOE-growth assays (growth in LB was monitored for 16h in the presence of 0, 1.25, 2.5, 5,
10 and 20 µg ml-1 of MOE). Essential genes were not tested, except as indicated in the Results.
Supplementary Table 2. Strains, plasmids and oligonucleotides. Bacterial strains used in this study Strains Relevant genotype References E. coli DH5α F‐ φ80lacZΔM15 Δ(lacZYAargF)U196 recA1 endA1 hsdR17 (rK‐,
mK+) phoA supE44 λ‐ thi1 Lab. stock
TG1 K-12 supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5, (rK-mK-) Lab. stock B. subtilis 168CA trpC2 Lab. stock DPVB45 ∆pbpG::kan 11
RE101 168CA ΔponA This work AG157 168CA ΔponA ΔpbpD ∆pbpF ∆pbpG::kan This work AG201 168CA ∆pbpG::erm This work AG221 168CA ∆ponA ∆pbpD ∆pbpF ∆pbpG::erm pLOSS-Pspac-ponA This work AG494 168CA ΔsigM::kan 28
AG831 AG221 ΔsigM::kan This work YK1342 168CA Ωneo3427 ΔmreD 16
YK1343 168CA ΔrodA::kan 16 YK1347 168CA Ωneo3427 ΔmreC kan 16 YK1334 168CA ΔponA::spc 24
YK2235 AG221 ΩrodA::kan-Pspac-rodA This work YK2239 168CA ΔponA ΔpbpD ΔpbpF ΔpbpG::erm This work YK2242 YK2239 ΩrodA::kan-Pspac-rodA This work YK2245 168CA ΩrodA::kan-Pspac-rodA This work YK2246 168CA ΔponA::spc ΩrodA::kan-Pspac-rodA This work YK2249 168CA amyE::Pxyl-rodA cat This work YK2250 168CA amyE::Pxyl-ftsW cat This work YK2251 168CA ΔponA::spc amyE::Pxyl-rodA cat This work YK2256 AG221 amyE::Pxyl-rodA cat This work YK2257 AG221 amyE::Pxyl-ftsW cat This work YK2259 AG221 amyE::Pxyl-rodA cat ΔsigM::kan This work YK2268 168CA amyE::Pxyl-rodA cat ΔsigM::kan This work YK2270 168CA amyE::Pxyl-mreBCD spc ΔmreC::kan 15
YK2271 168CA amyE::Pxyl-mreBCD spc ΔmreD::kan 15
YK2272 168CA amyE::Pxyl-mreBCD spc ΔmreC::kan ΔponA This work YK2273 168CA amyE::Pxyl-mreBCD spc ΔmreD::kan ΔponA This work YK2274 168CA amyE::Pxyl-mreBCD 15
YK2275 168CA amyE::Pxyl-mreBCD spc ΔponA This work YK2276 168CA ΔsigM::kan amyE::Pxyl-mreBCD spc This work B. subtilis strains used for MOE sensitivity test
AG832 168CA ΔylxW::erm Koo BM et al., in preparation AG833 168CA ΔylxX::erm Koo BM et al., in preparation AG834 168CA Δsbp::erm Koo BM et al., in preparation AG835 168CA ΔyqjL::erm Koo BM et al., in preparation AG836 168CA ΔytpA::erm Koo BM et al., in preparation AG837 168CA ΔyceC::erm Koo BM et al., in preparation AG838 168CA ΔyceD::erm Koo BM et al., in preparation AG839 168CA ΔyceE::erm Koo BM et al., in preparation AG840 168CA ΔypbG::erm Koo BM et al., in preparation AG851 168CA ΔypuD::erm Koo BM et al., in preparation AG852 168CA ΔyqiG::erm Koo BM et al., in preparation AG853 168CA ΔyxeF::erm Koo BM et al., in preparation AG856 168CA ΔyceH::erm Koo BM et al., in preparation AG857 168CA ΔyceF::erm Koo BM et al., in preparation AG858 168CA ΔyfiC::erm Koo BM et al., in preparation AG859 168CA ΔylzH::erm Koo BM et al., in preparation
AG860 168CA ΔyrzF::erm Koo BM et al., in preparation AG861 168CA ΔywbG::erm Koo BM et al., in preparation AG862 168CA ΔywzG::erm Koo BM et al., in preparation AG863 168CA ΔydfM::erm Koo BM et al., in preparation AG864 168CA ΔyrhH::erm Koo BM et al., in preparation AG865 168CA ΔyfiN::erm Koo BM et al., in preparation AG881 168CA ΔycgQ::erm Koo BM et al., in preparation AG882 168CA ΔycgR::erm Koo BM et al., in preparation AG883 168CA ΔyjbC::erm Koo BM et al., in preparation AG884 168CA ΔylaA::erm Koo BM et al., in preparation AG885 168CA Δamj::erm Koo BM et al., in preparation AG886 168CA ΔyrhL::erm Koo BM et al., in preparation AG887 168CA ΔytrB::erm Koo BM et al., in preparation AG888 168CA ΔywbO::erm Koo BM et al., in preparation AG1085 168CA ΔyceG::erm Koo BM et al., in preparation AG1086 168CA ΔywaC::erm Koo BM et al., in preparation AG1087 168CA ΔyacL::erm Koo BM et al., in preparation AG1088 168CA ΔyebC::erm Koo BM et al., in preparation AG1089 168CA Δabh::erm Koo BM et al., in preparation AG1090 168CA ΔyacC::erm Koo BM et al., in preparation AG1091 168CA ΔypuA::erm Koo BM et al., in preparation AG1092 168CA ΔyngC::erm Koo BM et al., in preparation AG1093 168CA ΔydcA::erm Koo BM et al., in preparation AG1094 168CA ΔbcrC::erm Koo BM et al., in preparation AG1095 168CA ΔyrhJ::erm Koo BM et al., in preparation AG1096 168CA ΔfatR::erm Koo BM et al., in preparation AG1097 168CA ΔywnJ::erm Koo BM et al., in preparation AG1098 168CA ΔyvrD::erm Koo BM et al., in preparation AG1100 168CA ΔyhbI::erm Koo BM et al., in preparation AG1161 168CA ∆minCD::kan 29
AG1174 168CA minD::erm 30
AG1245 168CA dltAB::cat 31
RE203 168CA ΔtagT::spc 16
AG612 168CA ΔltaSA::erm 32
cat, chloramphenicol; tet, tetracyclin; erm, erythromycin; spc, specctinomycin; kan, kanamycin; bla, β-lactamase; represent resistant genes, respectively.
Plasmids used in this study Plasmids Relevant features References pMUTIN4 used as matrix to amplify erm 33
pG+host9 thermosensitive replication, erm 23
pG+host10 pG+host9, bla This study pG+host9::ΔponA carry flanking DNA regions of ponA This study pG+host10::ΔpbpD carry flanking DNA regions of pbpD This study pG+host10::ΔpbpF carry flanking DNA regions of pbpF This study pLOSS* bla spc Pspac-mcs PdivIVA-lacZ lacI reppLS20(GA→CC) 24
pLOSS-Pspac-ponA bla spc Pspac-ponA PdivIVA-lacZ lacI reppLS20(GA→CC) This study pSG441 bla aph-A3 lacI Pspac 34
pRD159 bla aph-A3 lacI Pspac-rodA’ This work pJPR1 bla amyE3’ cat Pxyl amyE5’ Lab stock pRD163 bla amyE3’ cat Pxyl–rodA amyE5’ This work pRD164 bla amyE3’ cat Pxyl–ftsW amyE5’ This work
erm, erythromycin resistance, bla, ampicillin resistance; spc, spectinomycin resistance; mcs, multiple cloning site; lacZ, β-galactosidase ; lacI, lactose repressor.
Primers used in this study Primers Sequence RE05 GACATCGATGACGAAAGGGCCTCGTGATACGC To insert bla into pGhost9 RE06 CTTACTCGAGCCACCGCTGGTAGCGGTGG To insert bla into pGhost9
RE01 GACGCGGCCGCCGAAGATGACTTAAACGAAACG ponA pGhost9 left flank For primer (NotI)
RE02 CTTAGAATTCCGTTAATCAACCTTTCGCACC ponA pGhost9 left flank Rev primer (EcoRI)
RE03 GACGAATTCCCGTCACCTTTGGGGTGATGGC ponA pGhost9 right flank For primer (EcoRI)
RE04 CTTAGGTACCCGCAAGCAAAAGAAGTGTTGGC ponA pGhost9 right flank Rev primer (KpnI)
RE07 GACGCGGCCGCGGCATTTTCAGCAACGGG pbpD pGhost9 left flank For primer (NotI)
RE08 CTTAGAATTCATTCTAGCCATATCGTA pbpD pGhost9 left flank Rev primer (EcoRI)
RE09 GACGAATTCTAAAGGATCGGAGGGGATATGG pbpD pGhost9 right flank For primer (EcoRI)
RE10 CTTAAAGCTTCTAGATTCTCAGGTTTCCGCCG pbpD pGhost10 right flank Rev primer (HindIII)
RE11 GACGCGGCCGCGCCAGTCTTGAAAGGCGCTTTCCG pbpF pGhost9 left flank For primer (NotI)
RE12 CTTAGAATTCTTCTAAAGGTAGTATGGG pbpF pGhost9 left flank Rev primer (EcoRI)
RE13 GACGAATTCGCGATTTTTTGAACTTTGAGC pbpF pGhost9 right flank For primer (EcoRI)
RE14 CTTAAAGCTTCTCAAGCACGTACGGCACGTCC pbpF pGhost10 right flank Rev primer (HindIII)
AG21B GGTAACGCCAGGGTTTTC To check insert in pGhost9 by sequencing
AG22 GTGCCACCTGACGTCTAA To check insert in pGhost9/10 by sequencing
AG23 CACGTTACTAAAGGGAATG To check insert in pGhost10 by sequencing
AG11 GACGCGGCCGCGAAAGGTTGAGATGTTATGTC To amplify ponA locus AG12 GGCACTAGTTAAAACGGGAGGTTTTTTATTGTG To amplify ponA locus AG18 GTTGACTTTATCTACAAGGT To check insert cloned in pLOSS* AG19 GGTACCAGTAGTTCACCAC To check insert cloned in pLOSS* AG124 CCATCATCTGGTGCGAAAGG To check B. subtilis ∆ponA AG125 CCGCAAAGCCGATTAATTGG To check B. subtilis ∆ponA AG126 TCTATTGGCGAGTGCTTC To check B. subtilis ∆pbpF AG127 AGCATCGACTCCGTATTG To check B. subtilis ∆pbpF AG128 TTATTCGGAATGGCGATGGG To check B. subtilis ∆pbpD AG129 CCTTAATGGCTGCAGTTGAC To check B. subtilis ∆pbpD AG203 GTACGTTCTAGAGTGTTCGTGCTGACTTGC To amplify erythromycin
cassette AG204 GGGGGATCCTTGGAAGCTGTCAGTAG To amplify erythromycin
cassette AG200-for GACAGGCCATGAAATTGGAG To amplify upstream pbpG AG114-rev GCTCTAGACATTGCATCCACAACGGGTTCC To amplify upstream pbpG AG201 CGGGATCCGGACAGAACCCGCAAACGTG To amplify downstream pbpG AG202 TGGGAGCTGCACCATAAAGG To amplify downstream pbpG AG130 AGAACGTGCTCCGGATTTGG To check B. subtilis ∆pbpG AG131 CGGCGACTGGGAGATGAAAG To check B. subtilis ∆pbpG AG338 TTCCATTGTGCCACTCCTTC To check B. subtilis ∆sigM AG339 GTTCGCTGTCAATCAGTTCC To check B. subtilis ∆sigM AG328 AAGTCTAGATAATGGAAGTTAGAAGCAGGGAAGAGGATG To amplify ftsW AG329 ATTAGCGGCCGCGGAATATCCTTCCCCTGTACAC To amplify ftsW AG330 ATGTCTAGATGAAAATGTAAGGCGGGATAGAATGAG To amplify rodA AG331 GATGCGGCCGCAGCCTCTGTAAAGGCTGTCTGC To amplify rodA
