A iosti ula t app oa hto edu i g st ess o you tu f

a d i p o i g tu f uality - ith i depe de t t ial data

Product Foliar Rate/Ha Active Benefits Cost/Ha

Phylgreen 15 Ascophyllum nodosum Improve root growth/heat tolerance 290

Alexin 5 Salicylic acid Improve ability of plant to fight disease 76

Delfan 2 24% amino acids; 9% N; 23% carbon Improve plant recovery 35

EDTA Ca 5 14% w/w calcium oxide (CaO) Harden cell wall 148

Silica 1-2 100% silica Harden turf 80-160

Green Fer Energy 5-8 21% iron EDDHA; 17% Humic acid Improve colour and root growth 80-161Monthly

Frequency

Monthly

Monthly

Monthly

Monthly

Monthly

Why is this approach beneficial ?

STRESS Ma age e t

Suppl of o ple sugars Suppl of a tio ida ts

Suppl i oligo-ele e ts

Suppl of Potassiu

Suppl i os oregula ts

MIX Ph lg ee WITH YOUR OTHER FOLIAR PRODUCT“ !

Appli atio : s all doses ut egula

CONTACT EFFECT

pH EFFECT

SYNERGIES ith Catio s Fe, Ca, Mg, M …

Effe ts: MAIN BENEFITS.

REDUCE stress effe ts fro soil a d eather o ditio s

E ha e faster re o er after frost, draught or sali it stress

I pro e utrie t a sorptio espe iall ke ele e ts Mai l atio s like K+, Ca +, Mg +, Fe +, Se+…

I rease i ro iologi al di ersit of the soils

Pseudomonas sp., Bacillus subtilis�

I pro e the root de elop e t i parti ular root le gth

Redu e da agi g effe ts of pathoge s a d pesti ide

treat e ts effe t a idi pH a d a tio ida ts

L A N D L A B

Drought Stress

La dla Resea h “tatio Qui to Vi e to Ital D . Ad ia o Altissi o Rossella Bo tolaso

T ials ea

- “oil: % sa d + % la dla

- epli ates / plot . – “tats ANOVA

- Lolium perenne & Festuca rubra

- 0 % (high stress), 25% (medium Stress) and 50% (low stress)

of Evapotranspiration (water restitution using sprinkler)

Drought Stress E peri e tal Setup:

Sprinkler for irrigation

- Spraying of 0 (control), 30 and 60 litres AG200 every 3 weeks

Para eters Assessed:

- % Li i g G ou d Co e LGC usi g Digital I age A al sis / eek

- Visual Tu f Qualit TQ afte ea h ut - Chlo oph ll o te t efo e e e ut

Chlorophyll Meter (CM) - Che k of the soil hu idit / eek

Drought Stress d Mai Out o e: R eg ass Lolium perenne – Be efits of phylgree

Co t ol L/Ha

-As the stress i rease, the TQ is de reasi g

.

.

.

.T

Q (

Tu

rf Q

ua

lity

)

Ryegrass -Turf Quality at high drought stress

Drought Stress d Mai Out o e: R eg ass Lolium perenne – Be efits of the phylgree

Co t ol L/Ha

As the st ess i ease, the Chlo oph ll o te t is de easi g

. . . . . . . . . . . . . . . . . .

CM

Ryegrass - Chlorophyll Measure (CM) at high drought stress

Drought Stress rd Mai Out o e: R eg ass Lolium perenne – Be efits of the phylgree

Co t ol L/Ha .

.

.

.

.

.

.

.

.

-“ep -“ep -O t -O t -O t -O t -No -No

% Dr Pat hes o R egrass after phylgree treat e t i high drought stress

Co t ol

15 L/ha

Drought Stress

Co trol 15 L/Ha R eg ass Lolium perenne – % ate estitutio ediu st ess – e d of t ial De e e

.9 .

. .

. .

% Gree

Chlo. M.

% D

Meloidogyne minor

Nematodes

“ pto s o a g ee …

Pi tu e ou tes of D . Coli Fle i g

RESULTS RELATION TO NEMATODES i e peri e ts ith AFBI,

I ease of Ne atode ates i o t ol a d significant de ease i Ne ati ide Mai Co lusio s:

AG pu e a d AG Roots a e li iti g de elop e t of e atodes at ost of appli atio s Effe t of o petitio et ee the i o ial life a d Ne atodes

Nematodes

Start of Autu Start of Spri g

Application:

Small doses but frequent

“t ess e ha i al

“t ess heat / ate

Possi le “t ess Fu gi

“t ess F ost

Possi le “t ess A so ptio

N, P

Oligo elts

K AG Hu i s AG Pu e

Use as ase produ t for a foliar spra i g – i ith other produ ts i parti ular fu gi ides

A atu al i of Biosti ula tsBiostimulant

Type/Familly

Active

compounds

Main

Origin

Action

Plant

Growth

Regulators

(PGR)

Plant hormones

Amino Acids

Humic acids

Sugars (Carbon)

Bacteria

Synthetic

Synthetic

Natural

Natural

Natural

Enhance development

of key phases:

Roots, leaf, flowers…

(direct or indirect)

Plant Defense

Stimulators

Auxines

Glutamic acid

Oligosaccharides

Synthetic

Both

Natural

Direct stimulation of

Plant defense system

Osmoregulants

Antioxidants

Glycin Betain

Mannitol

Salicylic acid

Vitamins, tannins

Natural

Natural

Both

Both

Help plant regulate

Water inside/outside

Neutralise free radicals

Biofungicides

Biopesticides

Trichoderma sp.

Garlic, Mustard…

Natural

Natural

Compete with pathos

Act on pest physiology

Seaweed

Cold Extracts -

Plant

Hormone like (+) ,

A.A. (+),

Sugars (+++)

Auxins (+),

Glutamic Acid (+)

Oligosaccharides (+++)

Mannitol (++),

Vitamins (+,

Tannins (++)

In addition:

Alginates (- charged) =

surfactant & water retention

Free amino acids – content of amino acids that can be used fast by the plant

Total amino acids – content of free and peptide amino acids. Only part of the total amino acids can be used

Peptides - short amino acid strains with two or more amino acids

Proteins - long chains of amino acids folded in a special way. Proteins have important functions in plant metabolism

Definitions

Delfan Plus is extremely high concentrated in free amino acids, 24%. Products based on enzymatic hydrolysis

have a lower content of free amino acids

Delfan Plus – technical specifications

PHYSICAL-CHEMICAL CHARACTERISTICS Aspect: Liquid Colour: Brown Density: 1,2 g/cc

pH: 7,2 CHEMICAL ANALYSIS

Parameter Guaranteed value (% w/w) Admitted deviation* Free aminoacids** 24,00% -1,20

Total nitrogen (N) 9,00% -0,90

Organic matter 37,00% -3,00 Organic carbon 23,00% -2,00

The importance of the aminogram

Delfan Plus aminogram

Histidine

Threonine

Methionine

Tryptophane

Valine

Arginine

Serine

Lysine

Phenylalanine

Isoleucine

Leucine

Aspartic acid

Tyrosine

Hydroxyproline

Proline

Alanine

Glycine

Glutamic acid

0

2

4

6

8

10

12

14

free

AA c

onte

nt [%

w/w

]

Vegetable aminogram vs. Delfan Plus

Typical vegetal AA from soybean and wheat Delfan Plus

No Hydroxiproline and little proline

Differences in aminograms

The importance of Proline and Hydroxiproline

Proline is an excellent anti stress agent and leads to tolerance to a wide range of abiotic stresses

Proline is especially important during osmotic stress (drought, salinity and freezing)

Proline leads to:

Osmotic adjustment (Compatible osmolyte)

Stabilized cellular structures

Prevention of cell damage due to free radicals (Radical Scavenger)

Provides energy needed for recovery

Protein synthesis

DNA synthesis

Precursors of hormones

Precursors of other functional molecules

Stress metabolism

Functions of amino acids in plants

Amino acids are the building blocks of life

Without amino acids there is no life

Functions of amino acids

Amino acid Effect or function All AA Protein synthesis Glycine DNA synthesis, Alcaloid metabolism Glutamic acid Chlorophyll synthesis Tryptophane Auxin and phytoalexin precursor Methionine Ethylen and polyamine precursor Aspartate, glutamine and glutamate

N and C storage amino acids, transport amino acids

Proline stress metabolism, flowering Serine Precursor glycine betaine, stress metabolism

Alanine Precursor of certain antibiotics in some species

Leucine, Lysine, tryptophane, histidine, phenylalanine, tyrosine and glycine

Alcaloid metabolism, plant protection against pests and stress

Phenylalanine Salicylic acid production, stress and disease prevention

Tyrosine Glucosinolate precursors “Phytoanticipins”

Methionine

Arginine

Tryptophane

Rest

Delfan Plus content of hormone precursor amino acids

Serine

Proline

Rest

Osmolyte content of Delfan Plus

34.5

35

35.5

36

36.5

37

37.5

Control Delfan

Yiel

d [T

/ha]

Yield of potatoes after Delfan applications of 1.5L/ha compared to standard in a dry year

Delfan effect on potatoes during dry conditions

Delfan and stress prevention

Delfan contains osmoprotectants proline, hydroxiproline and serine

Better tolerance to saline conditions

Protection against dry spells

Enhanced cold hardiness

Enhanced yield

Introduction

Need for more proof Years of independent trial data Consistent, even application of nutrient across turf surface Faster establishment of new turf Enhanced turf quality Improved soil and plant fertility Less leaching of nutrient from the root zone Saving to fertiliser budgets often achievable Simple, integrated plan Easy to use, clean products – boom or fertigation applied

90

Years of R&D done in Broad Acre Ag

Trial Data Unique to Microsoil (broad acre ag) & a focus of Microturf Most trial work has been done via boom application Independent proof of products’ ability to enhance plant nutrition Identified best practice with liquids With or without granular fertiliser

Ongoing trial work is integral to ongoing product development Highlighted inefficiencies of granular fertiliser

LIQUID INJECT TRIAL

97

Treatment Yield T/ha Protein % Net Gain/ha on MAP

SFP =80kg MAP 3.02 8.7% 0

MICROSOIL CROP STARTER + 35kg MAP + AWAKEN ST 3.60 10.3% $179.15

AWAKEN ST + 80kg MAP 3.11 10.3% $20.20

AWAKEN ST + 80kg MAP + AWAKEN 3.37 10.3% $81

AWAKEN ST + 80kg MAP + NUTRISYNC M 3.16 8.1% $23.25

AWAKEN ST +80kg MAP + MAXIMUM N PACT 3.15 10.2% -$1.60

WHEAT price = $280/T

2010-2012 Turf Trial (Buffalo) Windsor NSW

Details: This long term trial was conducted over some difficult growing seasons in Sydney. The Turf business set key indicators for trial success as being: improved turf and soil quality; greater speed to market; reduced fertiliser costs/more efficient use of applied nutrients; a more sustainable nutrition plan with less emissions and increased soil carbon and finally faster establishment of the following turf. A one HA block was used for the trial, half of which continued to have applied an industry standard turf program of synthetic fertiliser costing approx $1,500/HA. 25% of the block received half that amount of standard synthetic fertiliser ($750/HA) along with Microturf’s range of liquid biological mineral fertilisers. The remaining 25% received no synthetic fertiliser and just the Microturf liquids. Comments from the Turf Manager were that throughout the seasons the areas ‘Treated’ with Microturf were visibly ahead in their development compared to the standard ‘Untreated’ areas of the block. This included the establishment and appearance of the turf and importantly how the turf cut. The subsequent turf receiving the same treatments continued to show marked improvements in the Microturf ‘Treated’ areas. A soil testing program was conducted throughout the trial and the results tell a very compelling story about nutrient efficiency and the ability to eliminate wastage from intensive fertiliser programs such as those commonly adopted in Turf and Horticulture programs. A snapshot of the soil tests can be seen here. Note that despite significantly less units of fertiliser being applied in the 'Treated' areas where Microturf's liquid fertilisers were used, these areas continually showed up higher levels of available nutrient when compared to the ‘Untreated’ areas that received synthetic fertiliser at $1500/HA . Organic matter and soil Carbon levels also increased in Microturf ‘Treated’ areas.

Sir Walter Trial Layout 2010-2012 Windsor NSW

U

1 HA trial block on Wilberforce Rd Windsor.

Sta

nd

ard

Sy

nth

eti

c F

ert

Pro

gra

m

½ S

tan

dard

Fert

Pro

gra

m +

Mic

rotu

rf

Mic

rotu

rf o

nly

Wilberforce Rd

Drive W

ay

STD kg/ha

N 264

P 78

K 210

S 102

Ca 38

Mg 18

101

Key Indicators

Summer Grass Dormancy Trial ACT (Buffalo) – 28th Sept 2012 Details: This trial was conducted over a 12 week period in Canberra where summer grasses often remain in a dormant state until the end of December. The trial aimed to gauge the success of a fast recovery from dormancy in conjunction with monitoring any improvements in turf conditions over a three month period to ensure that any gains were not short lived. The Microturf trial products were applied to buffalo grass on the 28th September 2012. The entire field and the ‘Control’ had received a standard application of granular synthetic fertiliser prior. The Microturf products applied were: PRO NITROGEN; PROMOTE, PRO CAL+ and PRONTO. The trial was monitored over 9 occasions during the 12 week period where a visual record of the turf colour, density and health was noted. A tissue sample was taken from each of the plots including the ‘Control’ on the 8th November 2012. Plots with Microturf liquid fertiliser applied consistently showed up improved nutrient levels over the Control in key areas testifying to Microturf’s ability to quickly enhance plant nutrition levels. Comments from the Turf Manager were that from the very first week of the trial onwards the plots treated with Microturf products were significantly ahead of the control in terms of colour and plant vigour. PRONTO was the initial standout performer with PROMOTE & PROCAL+ showing improvement from week two followed by PRO NITROGEN in week three. PROMOTE showed the best longevity throughout the 12 week trial with all plots coming back to an even state as conditions warmed up at the 12 week period.

PHOTOS TAKEN 1ST November 2012 Summer Grass Dormancy Trial ACT – 28th Sept

PLOT 5 PLOT 8

PLOT 4 PLOT 1

PLANT TISSUE REPORT - BUFFALO DORMANCY RECOVERY TRIAL

SITE: Canberra

Teatment applied 28th September 2012

Plot 1 Plot 4 Plot 5 Plot 8 Control Tissue Test Date 8/11/2012 8/11/2012 8/11/2012 8/11/2012 8/11/2012 MICROTURF PRODUCT

Plant Tissue Analysis Nitrogen 2.22 1.69 2.64 1.63 2.01 Phosphorus 0.39 0.32 0.36 0.34 0.29 Potassium 1.5 1.3 1.4 1.5 1.2 Sulphur 0.30 0.27 0.33 0.29 0.31 Calcium 0.40 0.29 0.44 0.28 0.41 Magnesium 0.24 0.19 0.25 0.2 0.21 Sodium 0.75 0.68 1.22 0.57 0.76 Iron 1782 1446 1407 1693 1127 Manganese 73 51 60 88 51 Zinc 90 48 57 124 56 Copper 14 10 13 12 10 Boron 22 21 20.1 18.8 16.2 Molybdenum 0.10 0.10 0.1 0.1 0.1

2012 Ribbon Trial (Buffalo) – Canberra ACT

Details: This trial was conducted over a 12 week period in Canberra where summer grasses often remain in a dormant state until the end of December. The trial aimed to gauge the success of a fast recovery from dormancy in conjunction with monitoring any improvements in turf conditions over a three month period to ensure that any gains were not short lived. The Microturf trial products were applied to buffalo runners on the 1st November 2012. The entire field and the ‘Control’ had received a standard application of granular synthetic fertiliser prior. The Microturf products were applied to four plots which were pegged out for trial measurement. The trial was monitored over a 10 week period where a visual record of the row closure was noted along with turf colour, density and health. Comments from the Turf Manager were that the plots treated with Microturf products were ahead of the control in terms of their development. Runners were staked out and in the treated areas they were shown to be more frequent and they grew ‘across the deck’ faster with less of a ‘dolphining’ effect. The demonstration also showed that areas treated with Microturf products had better colour in ribbons with greater biomass.

PRODUCTS APPLIED 1ST November 2012 Ribbon Trial (Buffalo) ACT

PLOT 5 PLOT 8

1ST NOV 19TH NOV

19TH NOV 19TH NOV

Sand Based Sports Fields Limited media for plant & root development Plus lot of irrigation Less leaching Pro Nitrogen Very happy with results Golf – greens stayed green longer Football – better root dev & turf recovery. Helped with

Rhizoctonia & Pythium issues & new emergence

A liquid organic nutrient complex containing

salicylate derivates to boost the immune response of the plant. Alexin can safely be included in a disease

management programme and is highly tank compatible.

Salycilic acid Plants manufacture salicylic acid to trigger natural defences against disease.

The addition of Salycilic acid acts as

an activator of ‘Systemic Acquired Resistance’

(SAR). However, plants often don’t produce

the acid quickly enough to prevent injury when

attacked by a pathogen and so spraying

Alexin enables the ability of the plant to fight against infection.

Plants have never been totally defenceless

against disease attack. The issue has been

that often plants do not recognize their microbial

attackers in time.

Spraying salicylic acid in the form of Alexin

puts their defences on high-alert against future

attacks.

Research has found:

• Salt stress (salty water) (Borsani et al., 2001). improved plant tolerance to heat on creeping bentgrass (Larkindale and Huang, 2004)

• Improved heat tolerance of Kentucky bluegrass in a