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    RECOMMENDATIONS FOR THE DESIGN AND MAINTENANCE OF SALMON DISPLAY AQUARIA

    PREPARED FOR: THE TECHNICAL SERVICES BRANCH, TOURISM NEW BRUNSWICK, FREDERICTON, N.B.

    MARINE RESEARCH ASSOCIATES, LORD.S COVE, DEER ISLAND, N. B.

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    FINAL REPORT TOTECHNICAL SERVICES BRANCHTOURISM NEW BRUNSWICKFREDERICTON, NEW BRUNSWICK

    RECOMMENDAnONSFORTHE DESIGN AND MAINTENANCEOF SALMON DISPLAY AQUARIA

    A.A. MacKay, DirectorMARINE RESEARCH ASSOCIATESLord's Cove, Deer Island, N.B.EOG 2JO

    Reference: NBT76-1Dated: June 1,1976

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    D. Steeves, Acting DirectorTechnical Services BranchTOURISM NEW BRUNSWICK,Fredericton, N.B.On May 5, 1976, Marine Research Associates was requested by the TechnicalServices Branch of Tourism New Brunswick to prepare designs and compile tech

    nical data for the construction and maintenance of salmon display aquaria forthe department's new visitors centres.Specifically, the agreement called for:

    I. A design for a self-contained aqua rium unit suitable for maintainingsalmon for display,2. A design for an aquarium, with satellite water control unit, suitablefor maintaining salmon for display,3. Cost estimates for the construction, delivery, and installation of the a bove units,4. Data on the operation and maintenance of the display aquaria, andS. Recommendations and costs for an instruction manual and/or touristinformation pamphlet.

    It is our pleasure to supply the information, outlined above, in the form ofthis report.

    ~ ~ ~ Arthur A. MacKay, Director,MARINE RESEARCH ASSOCIATES,Lord's Cove, Deer Island, N.B.EOG 2JO

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    INTRODUCTION

    Successful maintenance of salmon, or any other organism, in a closed watersystem is largely a matter of maintaining water quality at a level which is withinthe acceptable range of the organism.Basically, the following parameters are those which are most important:1. OXYGEN LEVEL. Since fish require large quantities of oxygen for respiration, systems design must include an aeration system which is adequate to serve

    the total quantity of fish in the system. This is readily accomplished by air pumpswhich diffuse air into the water.2. FILTRATION. In a closed system, nitrogenous wastes, excreted by the fish,gradually build to toxic levels unless some mechan ism is included in the designwhich removes or degrades these products. In addition, there is a tendency towards the build-up of particulate matter and organic debris which greatly reducesthe attractiveness of the display aquarium. In small systems, mechanical filtration

    is accomplished through the use of charcoal and glass wool filters. However, theseare not practicable for large systems where subgravel filtration is usually usedfor both mechanical and biological filtration. Water is drawn through gravel on aperforated filter plate. In doing so, particulate matter is filtered out of the water.The most important function of the filter is, however, biological filtration. Ammonia is the major metabolic waste of most aquatic animals and is extremely toxicto most species. Salmon are sensitive to concentrations as low as 0.006 parts permillion (J .M. King and W.E. Kelley, Marine Aquariums: Principle and Prae/ice.

    Aquarium Systems, 1974). Fortunately, nitrifying bacteria are common in bothsalt and fresh water and, in sufficient numbers, they oxidize toxic ammonia tonitrite and finally to non-toxic nitrate. The filtration gravel provides large surfacearea for the growth of these bacteria and it is here that the toxic metabolic wastesare reduced to non-toxic products.

    3. pH. Changes in pH, from recommended levels, indicates a change in waterquality which can be detrimental to the aquarium animals. pH is maintained bycontinuously percolating water through a soluable calcareous gravel. As a result,selection of the proper filtration gravel is vital. This material is readily availablecommercially.

    4. OTHER ORGANIC AND INORGANIC IONS. Other organic and inorganicmaterials tend to build-up in a closed system. While most are not toxic, they willaffect the display organisms and reduce the attractiveness of the aquarium. Amonthly change of water (25 percent of the total volume) will usually eliminatethis problem.

    5. TEMPERATURE. All organisms have a more or less fixed range of suitabletemperature which must be maintained by refrigeration and heating systems.

    The designs which are presented in the following pages all incorporate mechanisms which will eliminate or reduce to a minimal level the problems outlinedabove.

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    We have attempted to provide as many options as possible for the selectionof the most suitable aquarium design. DESIGN ONE incorporates two separateunits; the display aquarium and the water management unit. The display aquarium has a limited number of working parts and is connected, by pipes, to awater , m a . ! J a ~ e m e n t unit which can be installed at a moderate distance from theaquarium, provided that it is on the same level.

    The water management unit is the key to the successful operation of the display aquarium. It maintains water temperature within 1 C degree of any desiredtemperature between 5 degrees and 25 degrees C. TIlis is accomplished throughsensor controlled refrigeration and heating systems. Filtration of water is bothmechanical and biological as previously described. Oxygen is provided by airpumps.

    DESIGN OPTION I-AThis design is illustrated in Figure I. Specifications are as follows:

    DISPLAY AQUARIUM: Constructed by Marine Research Associates.SIZE: 3'Wx4'Hx8'LWATER FLOW: Moderate laminar flow through end entry and exit.700 gallons per hour.ACCESSORY AIR: Provided by single "Little Giant" air pump inlight box.SUBGRAVEL FILTRATION: Minimal removal of particulate matterwith subgravel filtration bed (perforated pvc pipe).MATERIALS: 1 inch resin coated marine plywood; finished exterior,light blue interior. Silicone sealant seals. Window Tx2'; 1/2 inch plateglass. 2x4 framing for ends and base. finished exterior.APPROXIMAT E CAPAC'lTY: 500 imp. gallons.WATER MANAGEMENT: WM500 Water Management Unit. Conunercial unit available through MRA. from Aquarium Systems Inc. Seespecifications supplied in Figure 2.

    DESIGN ONEDISPLAY AQUARIUM WITH SATELLITEWATER MANAGEMENT UNIT

    DESIGN OPTION I-BThe design of this unit is basically the same as shown in Figure 1 with modifications as shown in Figure 3. It is based upon designs for custom construction

    solicited by Marine Research Associates from Aquarium Systems Inc; a companywith which we have had a long association. They are one of North America'sleading designers and manufacturers of closed system salt and freshwater aquaria.DISPLAY AQUARIUM: Constructed by Aquarium Systems Incorporated.

    SIZE: 4'Wx3'Hx8' LONG.WATER FLOW: Moderate surface laminar flow at entry. Additionalflow provided by 4 patented corner airlifts operated by 4 independentair pumps. 700 gal. per hour.

    ACCESSORY AIR: As outlined above.

    SUBGRAVEL FILTRATION: As previously described. 800 pounds ofhigh grade filter gravel included with unit.MATERIALS: 1" H.D.O. Marine plywood impregnated with plastic.Blue epoxy finish inside. Offwhite plastic laminate exterior. Siliconesealant seals. Window 7 feet x 2 feet; 1/2 inch plate glass.APPROXIMATE CAPACITY: 500 imp. gallons.WATER MANAGEMENT: WM-500 water management unit as previously described (Figure 2) including 12 foot hoses for inlet and outlet toaquarium.

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    DESIGN OPTION I-CThis unit uses commercially produced components, available from Aquarium

    Systems, which have been modified for the display of salmon. The display aquariumhas about one-half the display capacity (250 gallons) of the previously describeddesigns.

    DISPLAY AQUARIUM: Stock aquarium redesigned specifically for displayof salmon (See Figure 4). Includes 12 foot hoses for connection to watermanagement unit, necessary PVC fittings amI modification, as well as highcapacity pump.SIZE: 61 " front, 831/2" back, 24" depth, 30 " heightWEIGHT (shipping : 225 Ibs. plus 300 Ibs. of fiftrant shipped separately.(when filled: 24001bs.FINISH AND MATERIAL: Reinforced fiberglass.

    WATER CIRCULATION: Two independent systems, each usingpatented air-lift, each with its own pump.TEMPERATURE RANGE: Ambient - no t refrigerated or heated.FILTER: 3 cubic feet of INSTANT OCEAN Marine Filter Mix, a fiftrant and calcareous buffering material.ACCESSORIES: Subfifter plate, Marine Aquariums in the ResearchLaboratory Manual (Includes Water Chemistry), Experiments UsingMarine Animals Manual, Operating Instructions, diarama, hydrometer/thermometer, INSTANT OCEAN Synthetic Sea Salts, and spare airdiffusers.OPTION: The CS-225 can be built with an External Refrigeration System which would have to be connected and charged after the CultureSystem was set up. The themostatically controlled temperature is 5degrees C to ambient, plus or minus I degree C.POWER REQUIREMENTS: AC, 110 volts, 60 hertz, 6 amperes(grounded plug)water management; WM-500 Water Management Unit as previouslydesl.:rihed (Figure 2).

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    )

    )

    CONTROLUNIT-:{]EATER

    WATER MANAGEMENT UNIT

    [;].COOLING COMPRESSORo

    IIIt PUMPVALVE~ 1I IIdIl1I -----VALVE

    VALVE

    TANKDRAINAGESYSTEM(to main plumbing)

    AIR

    VALVE

    VALVE

    - ~ M E R G E N C OVERFLOW(to mainplumbing)

    SUBGRA VEL FILTER PIPES

    DISPLAY AQUARIUM

    t

    [ I t' I] t. pI I [ I ~ ~ I ~ . o " " ~ ~ . _ . . . . ~ ~ . - 0.. ........ _.. - .. FILTER BED

    FIGURE 1 . DESIGN ONE. SCHEMATIC SHOWING WATER CIRCULATION ANI> WATER MANAGEMENT SYSTEMS. Components are positioned for illustration only.4

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    SOO-Gallon INSTANT OCEAN:1" WabModel WM-500

    Th e Water Management Uni t is designed to autolsalt or f resh water to and from accessory aquaria 0gal lons total capaci ty. When connected to on e or meth e necessary parameters . . . aeration, f i l t rat ion,circulat ion, buffer ing, plus water temperature mainTh e WM-500 is constructed of marine plywood coverdecorative laminated plastic and f inished on th e insiing. All mater ials in contact with the water are no rThis system is ideal fo r managing the water in lar!of research animals, e.g. tw o 250'gallon tanks for senected to the WM-500.Th e Water Management Uni t ca n be easi ly coupledin g a dual manifold to th e outlet pipe_ This uni tseries o f smal ler aquariums.On e of the most important i tems suppl ied with each" System is intangible. As the oldest an d largest dmarine culture systems, th e professional staf f atassociate companies wil l , upon request, provide a brance in specf lc areas of aquatic cu I ture.SPECIFICATIONS:DIMENSIONS: 24" deep x 48 " wide x 60" high

    WEIGHT (sh ipping) : 825 Ibs.(when f i l led) : 1725 Ibs.

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    FINISH AN D MATERIAL : Antique whitecolored high- impact laminated p lastic on marine plywoolW AT ER C I R C U LAT I ON : Magneticallv-coupled plastic pump (700 gallons/hr.)REFRIGERATION SYSTEM: The latest innovation in culture refr igerat ion, nontoxic, polyethylene

    which el iminates the need for troublesome plastic coi ls which often cause a loss of freon. This sycompressor which is self. lubr icating and hermetical ly sealed.

    T EM PER AT U R E RANGE: 5 . 25 c C. Thermostat ical ly control led ! l 0CFILTER: 4 cubic feet of INSTANT O C E A N ~ r Marine Fi l te r Mix, a f i l t ran t and calcareous bufferingOPT I ON AL ACCESSORIES: Alterat ions fo r power requirements other than 110 V. 60 Hz .POWER REQUIREMENTS: AC , 110 volts, 60 hertz, 20 amperes (grounded plug).

    FIGURE 2. AQUARIUM SYSTEMS 500 GALLON WATER MANAGEMENT UNIT.5

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    AIR LIFT

    1\

    t 0

    J

    AI R LIFT

    / " -" ,-

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    225-Gallon Trapezoidal INSTANT OCEAN Culture SystemModel CS-225

    Th e CS-225 wa s original ly developed fo r th e Aquarium of Niagara Fal ls, theworld's f i rst inland "oceanarium." Similar systems are also being employed atthe Myst ic Marinel i fe Aquarium, Aquarium of Cape Cod and, at Hawai ian MarineImports, Houston, Texas.This system is entirely ~ e l f c o n t a i n e d an d provides rel iable control over all thenecessary requirements for successful marine culture. These include physicalf i l t rat ion, biological removal of toxic ammonia an d nitrite, continuous bufferingof the sea water, automatic replenishment of the alkal ine reserve, an d maintenance of proper 02 an d C0 2 levels.

    Two air- l i f ts are used to circulate th e water up to eight turnovers an hour. This unique circulat ion system requires no water pumpan d has no moving parts. Mechanical breakdown is therefore virtual ly impossible. The overal l method of water management wasdesigned an d patented by Aquarium Systems, Inc. in Mid-1968 under U.S. Patent No. 3,387,587. There ar e no licensees.The trapezoidal shape of the CS-225 makes it ideal fo r display use. Unique to this aquarium is th e "dry " rear diarama in whicha real ist ic and colorful display ca n be constructed. When viewed through th e front window th e tank sides and back disappear,giving th e i l lusion of great depth.

    FIGURE 4. AQUARIUM SYSTEMS' TRAPEZOIDAL DISPLAY AQUARIUM.

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    DESIGN OPTION 2-AStock commercial unit (Figure 5) from Aquarium Systems. The maximumcapacity of this unit is 150 gallons and, consequently, the number and size ofsalmon which could be placed on display is limited.

    SIZE: (overall) 25" deep x 481/2" wide x 51" high(aquarium): 24" deep x 47-1/2" wide x IT ' highWEIGHT: (shipping): 410 Ibs. plus 250 Ibs. of filtrant shipped separately. (when filled): 19001bs.FINISII AND MATERIAL: Antique white-colored, high-impact, laminated plastic on marine plywood.WATER CIRCULATION: Patented, air-lift system with dual diffusiondevices and three air pumps.REFRIGERATION SYSTEM: The latest innovation in Culture Systemrefrigeration, non-toxic polyethylene covered copper cooling coils eliminating the need for troublesome plastic coils which often cause a lossof freon. This system is powered by a 1/2 h.p. compressor which is selflubricating and hermetically sealed.TEMPERATURE RANGE: 3 degrees C to ambient. Thermosta tic control, plus or minus 1 degree C.FILTER: 2-1/2 cubic feet of INSTANT OCEAN Marine Filter Mix, a filtran t and calcareous buffering material.ACCESSORIES: Subf ilter plate, three glass cover plates, hydromete r/thermometer, Marine Aquariums in the Research Laboratory Manual(Includes Water Chemistry), Experiments Using Marine Animals Manual,Operating Instructions, INSTANT OCEAN Synthetic Sea Salts andspare air diffusers.OPTIONAL ACCESSORIES: Six section tank dividers, unassembled.Alternations for power requirements other than 110 V. 60 liz.POWER REQUIREMENTS: AC, 110 volts, 60 Hertz, 15 amperes(grounded plug).

    DESIGN TWOSELF-CONTAINED OISPLAY AQUARIUM WITHWATER MANAGEMENT UNIT

    A self-contained aquarium incorporates all of the features previously described.However, all working parts are built into the base, under the display aquarium.

    DESIGN OPTION 2-13This unit was designed and, if chosen, would be manufactured by Marine Re

    search Associates. It includes all necessary water management systems as shownin Figure 6. Specifications are as follows:DISPLAY AQUARIUM:

    SIZE: 3'Wx3'Hx8'LWATER FLOW: Moderate laminar flow through end entry and exit.700 gallons per hour.AERATION: Provided by air pump in base.SUBGRA VEL FI LTRAnON: Minimal removal of particulate matterwith subgravel filtration bed (perforated PVC pipe).MATERIALS: I inch resin coated marine plywood; finished exterior ,light blue interior. Silicone sealant seals. Window 7 feet x 2 feet; 1/2inch plate glass. 2x4 framing for ends and base, finished exterior.APPROXIMATE CAPACITY: 500 imp. gallons.WATER MANAGEMENT: Custom installation of components in aquarium base. Includes filtration bed, water pump, aerator, refrigeratorcompressor, and heater.

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    150-Gallon INSTANT OCEAN@ Culture SystemModel CS-150

    This large and handsome floor-based unit is entirely self-

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    I ILUORESCENT LIGHT FIXTURE

    [I>1I o ()(5oCo "6 10 OUTLET

    ('flECKVALVE

    "

    VALVE

    AIR

    -,...

    " 0

    VALVEDRAINAGE(to main plumbing)

    l '

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    The advantages and disadvantages of the various designs outlined in the previous sections are as follows:

    DESIGN OPTION I-A50 0 gallon aquarium custom manufactured by MRA with satellite Aquarium

    Systems Water Management Unit.ADVANTAGES:

    I. Water volume is adequate for maintainence of a number of salmon in asize range to about 20 pounds.

    2 . ~ T h e Unit provides moderate laminar flow of water which may simulatea flowing stream.

    3. Accessory water management systems are incorporated into the aquar ium design.

    4. The Water Management Unit provides all necessary equipment for main tenance of water quality in a 500 g

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    DESIGN OPTION I-C250 gallon stock display aquarium coupled to a stock water manangement

    unit. Manufactured by Aquarium Systems.ADV ANT AGES:

    This design option has the same advantages as outlined above and is less expensive than previous units.DISADVANTAGES:

    I. Display gallonage is only 250 gallons. Consequently, the number andsize of fish would be approximately one-half the capacity of the previous units.

    DESIGN OPTION 2-AA stock self-contained unit available from Aquarium Systems.ADVANTAGESThis unit has most of the advantages described for design option I-B.DISADVANTAGES:

    I. The unit has a total maximum capacity of only 150 gallons. Consequently, the numbers and sizes of salmon would be greatly reduced.2. Maintenance must be carried out in the display area.

    DESIGN OPTION 2-BA custom combined aquarium and water management un it manufactured byMarine Research Associates.ADV ANTAGES:

    This unit has all of the advantages descrihed for Design Option I-A.DISADV ANTACES:

    I. Approximately one week is required lo r toxicity tests.

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    2. Construction is more difficult for this unit than any of the others anddelivery by July ISis doubtful.3. Pumps and compressors are located directly under the aquarium andmay be annoying to the viewer.4. Water test, water changes, and general maintenance must be carried

    out in the display area. This may interfere with the daily routine at the Centre.RECOMMENDATIONS-SELECTION OF UNIT

    Since time is a critical factor, particularly during the current year, we wouldrecommend that Tourism New Brunswick select Design Option I-A, a 500 gallondisplay aquarium manufactured by MRA with a water management unit manufactured by Aquarium Systems and construction or"Iight box, base, and installation by Marine Research Associates in co-operation with Tourism New Brunswick.

    Our second selection would be Design Option I-B, a 500 gallon display aquarium manufactured by Aquarium Systems coupled with their WM-500 WaterManagement Unit.LAYOUT

    Suggested layout and general form of the exhibit are illustrated in the presentational drawing, which accompanies this report and, to scale, in Figure 7.We have made some changes from the layouts provided us by Tourism N.B. Ifpossible, we feel that the display aquarium and the "Leaping Salmon" displays;,ould be interchanged. This will place the aquarium closer to the room in whichthe water management unit is kept; thus reducing the problems involved in connecting the two units.Sufficient room is available above the display aquarium for an informationboard. This is not shown on the presentational drawing. However, this should beconsidered if it will "fit in" with other exhibits in the room.Choice of tank width is important. All of the custom-made aquaria can bebuilt three or four feet in depth. A deeper aquarium is probably more suitablesince optical effect will cause the tank to appear shallower than it really is.Figure 7 shows, to scale, the position of three foot and four foot wide aquariain relation to the display area.

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    "LEAPING SALMON"DISPLAYDISPLAY AQUARIUM

    Front 3 f1. wide tank

    WATERMANAGEMENTUNIT

    I_ L _ _ _ Front 4 f1. wide tank_______ ~ -=. --=--=- -= __)

    RESTIGOUCHE ROOM

    -= =_--= - JI

    ,)

    )FIGURE 7. PROPOSED 1./\YOUr 01; /\QU/\RIUM UNITS. Scale: I em equals 1 foot.

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    TRANSPORTING ATLANTIC SALMONWe would recommend, jf possible, that arrangements for the transportat ion

    of the salmon be made with experienced hatchery personnel. If this is not possible, we would recommenu:I. that pick-up of the fish be arrangeu so that transportation is accomplisheu in the shortest possible time,2. that containers be large enough to allow adult salmon only restrictedmovement and, if possible, that they be lined with a soft material such as foamsheet, rubber sheet, or styrofoam, anu firmly covered with similar material anu,3. that, if transportation is to take longer than 60 minutes, methods beconsidered to keep water temperature uown, and to provide aeration. Frozengel refrigerants are iueal for maintaining cool water temperature. A small cyIinuer of compressed oxygen, fitteu with air lines and diffuser stones, will provide auequate aeration.

    MAINTAINING ATLANTIC SALMONa) NUMBERS AND SIZE: We woulu recommend the following assortment

    of salmon:6 adult fish: 2 -20 Ibs. (I male, I female)2 -10 Ibs. (I male, 1 female)2 (;rilsc, 3-5 Ibs. (I male, I female)12 Smolt size ( I5-1 (j cm)

    ('ommcnts: This sclcctilln givcs goou representation of year classes. Males andfcmales shoulu he typical (i.c. malurc males with hooked jaw, females with wellroundeu heaus). Small juvellilcs shoiliu he avoiued since they compete poorlyfor feeu anu may he ealcil hy the larger fish. Earlier stages can he shown in drawings and/or preserveu specilllcllS. (bcellcnt graphics are available for the Atlantic Salmon hHllluation. Additiollal information is available from MRA if requireu).

    RECOMMENDATIONSB. TRANSPORTING AND MAINTAINING ATLANTICSALMON IN DlSPLAY AQUARIA

    b) MATERIALS IN AQUARIUM:).to 2 inch beach gravel with occasionallarge rounded rock. Sharp rocks should be avoided since adult salmon willscrape off their scales. Juveniles will hide behind very large rocks.Dark gravel shoulu be used as salmon will lighten in response to light reOectedfrom a light bottom and will look washed out rather than darkbacked and silversided.All gravel should he washed to remove organic material that may colonizeanu clog the system.When salmon are introduced into the aquarium, a soft aquarium cover (rubber sheet, foam pad, etc) should be finnly attached to the top, since the salmonwill jump for the first night or so. This will prevent damage to the fish untilthey settle in.At least six inches should be alloweu between the water surface and the aquarium cover to allow thc salmon to gulp air and fill their air biadders as required.c) WATER QUALITY REQUIREMENTS: Various water quality parametersshould be monitored regularly (at least weekly).pH shoulu be maintained between 6.5 to 7.5.6.8 to 7.0 is excellent. Changesfrom these values indica te a problem in the system.A salinity value of 5 to 6 parts per thousand will keep and make the salmonsilvery, as well as control fungus. This can be establisheu during set-up and akit provided for subsequent water changes.Temperature should be between 40 to SO degrees F with a maximum of 55uegrees F if conde!lsation is a problem.Dissolved gas levels (particularly oxygen) shoulu be held between 80 percent

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    and less than 100 percent. Both too little dissolved gas and supersatu ration willcause probehns. Excessive quantities of fine bubbles on inside aquarium surfacessignals trouble.

    Ammonia levels should be below O. I mgjliter. Partial water change shouldbe made at about 0.5 mgjliter.

    About 25 percent of the aquarium water should be changed monthly or ifwater becomes turbid or pH or ammonia levels fall outside acceptable limits. Ifbuilding water can be used, this would reduce problems with water changes.MRA can arrange for water tests.d) FEEDING: Fish should be provided a maintenance diet only. Adults donot require feeding. Feed smolts I percent of body weight per day (12 smoltsx30 grams equals 360 grams xO.OI equals 3.6 grams per day). Only feed what isreadily eaten. 1) 0 not allow feed to rest on bottom and remove uneaten food. Re duce quantity to level where all food is eaten if water becomes discolored orstop feeding until system clears.Feed can be "borrowed" from Charlo hatchery. We suggest No.4 Silvercupor Ewos brand feeds. Avoid "fines" (dust) in feed.If any fish loose weight drastically, they should be replaced. When re placing fish. be careful not to disturb them unnecessarily or they will damagethemselves.e) DISEASE: Should any fish show symptoms of disease, they should be re placed. I f disease persists contact:

    Environment CanadaFisheries and Marine ServiceFish Health LaboratoryWater Str eet,Box 550. Halifax, N.S.Attn: Dr. R. MacKelvie or Dr. J. Weber

    A complele change of water will likely be necessary.The following should be considered as symptoms of disease: swimming onback; reddening of ventral surface. particularly on belly and around vent;lesions (sores) and; s tahility problems.

    f) HANDLING: Take great care when netting fish. Adults should be removedby gently grasping the tail.Marine Research Associates is prepared to supply additional information andadvice during the summer display period. Inquiries should be directed to:

    Arthur MacKay, DirectorMarine Research Associates,Lord's Cove, Deer Island, N.B.Phone: 747-2201

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    INSTRUCTION MANUAL

    Since operators will be inexperienced, some fonn of simple, straightforwardinstruction manual should be available. Basic information on the life historyand habits of the salmon should be included since it will help the operator tounderstand why he or she is performing particular duties; some of which arecritical to the success of the display.Marine Research Associates is prepared to write and/or print such an in struction manual. Since some of the information will be of general interest totourists it would be possible to print an "over-run" excluding instructional material for distribution to the visiting public.We would estimate a need for a 16 page booklet, plus cover stock, measuring 5-1/2 x 8-1/2 inches. The tentative table of contents is as follows:

    I. INTRODUCTION2. IMPORTANCEa) Commercial Fisheryb) Sport Fishery3. LIFE HISTORY4. TilE RESTIGOUCHE SALMON5. INSTRUCTIONS FOR THE CARE AND MAINTAINENCE OFSALMON16

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    QUOTATIONS

    DESIGN OPTION I-ADisplay aquarium as described (M KA)Light box and aquarium cover (M KA)Base cabinet (M KA)Water Management Unit (ASI)Delivery and Installation (M RA)(2 men4 days)TOTAL QUOTE (Federal and Provincial taxes,duty, and Express charges for deliveryextra asapplicable).

    DESIGN OPTION 1-13Display aquarium as described (ASI) Light box and aquarium cover (MRA)Base cabinet (MRA)Water Management Unit (AS!)Delivery and Installation (M RA)TOTAL QUOTE (Federal and Provincial taxes.duty, and Express charges for delivery fromASI extra as applicable).

    DESIGN OPTION 1-('Display aquarium as describedand one Water Management Unit (ASI)Light Box and Aquarium cover (MRA)Base cabinet (M RA)Delivery and Installation (MRA)TOTAL QUOTE (federal and Provincial t'lxes.duty. and Express charges for delivery fromASI extra as applicable).

    3000.00350.00450.001975.00800.00

    (,575.00

    3500.00350.00450.001975.00800.00

    7075.00

    3725.00350.00450.00800.00

    5325.00

    DESIGN OI'TION 2-ADisplay aquarium including Water Managementsystems, base cabinet and cover as described (ASI)Delivery and Installation (MRA) 2450.00600.00TOTAL QUOTE (Federal and Provincial taxes,duty, and Express charges for delivery fromASI extra as applicable). 3050.00

    DESIGN OPTION 2-BDisplay aquarium including Water Managementsystems, base cabinet, and cover as described (MRA) 7100.00Delivery and Installation (M RA) 600.00TOTAL QUOTE (Federal and Provincial taxesextra as applicable. 7700.00

    WARRANTYMRA warrants all units to be free from defects in workmanship and materials.The liability of MRA is limited to repair or replacement and does not extend todamage or malfunction due to aCCident, abuse or misuse, or parts which havebeen disassembled or repaired.MRA shall not be liable for conseq uential damages for any breach of warranty.ASI units are covered by a similar warranty which accompanies their products.

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    MISCELLANEOUS MATERIALS AND EQUIPMENTI. 2 NETS (Nylon with Bamboo handle, 10") 2.50 ea.2. 12 AQUARIUM SCRAPERS3. 2 SIPHON CLEANERS 4.00 ea.4. 2 HYDROM HERS5. WATER QUALITY TEST KIT (estimate)6. MISCELLANEOUS CONTAINERS FO RWATER, etc.ESTIMATED TOTAL COST

    INSTRUCTION MANUALComplete as outlined:Preparation and final copy

    Printing (I color) 200 copies500 copies1000 copies

    Instruction sheet only:Final copy for photocopy orprintingPrinted (i f required) 100 copies

    200 copies500 copies1000 copies

    5.0010.008.00

    34.0080.0050.00

    187.00

    600.00110.00175.00260.00

    200.0018.5022.9535.9057.35

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