Urinary Tract Infections and Asymptomatic Bacteriuria in Pregnancy
Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
Transcript of Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
1/31
JYOTSNA
BBT FINAL YEAR
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
2/31
DEFINITION
Presence of bacteria >105 CFU/ml of
urine of a single pathogen in two
consecutive midstream, clean-catch
urine specimen without symptoms of
urinary tract infection.
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
3/31
CAUSES
According to Williams, progesterone
causes atonia of smooth muscles during
pregnancy. If the smooth muscles of the
genitourinary tract are affected, atonia
will lead to stasis of the urinary out flow
and infection would follow.
Incidence also depends on age, parity,race and socio-economic status.
pH of urine
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
4/31
1)To detect asymptomatic bacteriuria in pregnantwomen attending gynecology OPD of Patna
Medical College and Hospital, Patna .
2)Isolation and identification of the causative
microorganism of asymptomatic bacteriuria.3)To find out the prevalence with respect to age,
parity and socio-economic status of the
pregnant women.
4)To study the sensitivity pattern of the
microorganism against different antimicrobials.
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
5/31
PLACE OF WORK
Department of microbiology, Patna
Medical College.
The present study comprised of 50cases attending the Medical College.
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
6/31
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
7/31
Urine of the asymptomatic pregnant
women
Cultured on Blood agar, Mac Conkey
agar and Nutrient agar
Colony counting and characterization isdone after incubation
>105CFU/ml of urine Significant growth
103
-105
CFU/ml of urine Growth of doubtful significance
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
8/31
E. COLIONMAC CONKEYAGAR
E.COLION BLOOD AGAR
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
9/31
KLEBSIELLA PNEUMONIA
ON MAC CONKEY AGARKLEBSIELLA PNEUMONIA
ON BLOOD AGAR
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
10/31
PROTEUS MIRABILIS ON
BLOOD AGAR PLATE
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
11/31
PSEUDOMONASAERUGINOSA ON MACCONKEY AGAR
PSEUDOMONASAERUGINOSA ONNUTRIENTAGAR
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
12/31
DIFFERENT BIOCHEMICAL
TESTS FOR IDENTIFICATION
OF MICRO-ORGANISM
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
13/31
CYTOCHROME OXIDASE TEST
1% solution of
tetramethyl-p-phenylene
diamine hydrochloride
Oxidase positive
Pseudomonadaceae
Oxidase negativeEnterobacteriaceae
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
14/31
CATALASE TEST
3% hydrogen peroxide
Catalase positive
Enterobacteriaceae
Pseudomonas
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
15/31
INDOLE TEST
0.5ml Kovacs reagent
Indole positive(red color)
E.coli
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
16/31
METHYLRED TEST
5 drops of Methyl red
reagent
Positive result(red color)
E.coli
Negative result(yellow
color)other
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
17/31
CITRATE TEST
Simmons citrate medium
is used
Citrate positive(blue color)
Klebsiella pneumonia
Proteusmirabilis
Citrate negative(no colorchange)
E.coli
Psedomonas aeruginosa
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
18/31
UREASE TEST
Christensens urease
media is used
Positive (pink red color)
Proteusmirabilis
Klebsiellapneumonia
Negative(no color changeor change to pale yellow)
E.coli
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
19/31
ANTIBIOTICSUSCEPTIBILITY
TESTING 3-5 well isolated colonies were touched
and emulsified in sterile saline.
Turbidity of suspension was matched toturbidity standard.
Using a sterile swab ,inoculation was doneon Mueller Hinton agar plate.
Antibiotic disc were evenly distributed onthe inoculated plate.
The plates were incubated overnight at37C .
The reading was taken on the next day.
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
20/31
RESULTS
AND
OBSERVATION
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
21/31
INCIDENCE OF ASYMPTOMATIC
BACTERIURIA
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
22/31
ISOLATED ORGANISM IN BACTERIURIA
CASES
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
23/31
CASES IN DIFFERENTAGE GROUPS
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
24/31
PARITYWISE DISTRIBUTION
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
25/31
SOCIO ECONOMICSTATUS
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
26/31
SENSITIVITY TO DIFFERENTANTIBIOTIC
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
27/31
RESISTANCE TO DIFFERENTANTIMICROBIAL
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
28/31
CONCLUSION
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
29/31
The incidence of asymptomatic bacteriuria in pregnancy was found to be
10%.
The age of patients ranged from 15-45 years, the highest percentage ofbacteriuric patient were from 15-25 yrs.
The incidence of asymptomatic bacteriuria was found to be increasing with
decreasing socio-economic status.
Among the infecting organism,
Escherichia coliwas taken to be standard
Cases were sensitive to Erythromycin,Norfloxacin
Cases were resistance toAmpicilin,Amoxycillin
If untreated and undetected,it may lead to acute or chronic infection and
eventual death.
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
30/31
REFERENCES
1. Abbott, G. D. 1972. Neonatal bacteriuria: a prospective study in 1,460 infants. Br. Med. J.1:267-269.
2. Alexander, D. N., G. M. Ederer, and J. M. Matsen. 1976.Evaluation of an adenosine 5'-
triphosphate assay as a screening method to detect significant bacteriuria. J. Clin.
Microbiol.3:42-46.
3. Bailey, R. R. 1973. Significance of coagulase-negative Staphylococcus in urine. J. Infect.
Dis. 127:179-182.
4. Bartlett, R. C., and R. S. Galen. 1983. Predictive value of urine culture. Am. J. Clin. Pathol.
79:756-757.
5. Bartlett, R. C., D. O'Niell, and J. C. McLaughlin. 1984. Detection of bacteriuria by leukocyte
esterase, nitrite, and the AutoMicrobic System. Am. J. Clin. Pathol. 82:683-687.
6. Bruce, A. W., W. S. Chadwick, W. S. Willett, and M. 0. Shaughnessy. 1981. The role ofchlamydia in genitourinary disease. J. Urol. 126:625-629.
7. Brumfitt, W. 1965. Urinary cell counts and their value. J. Clin. Pathol. 18:550-553.
8. Brunner, H., W. Weidner, and H. G. Schiefer. 1983. Studies of the role of Ureaplasma
urealyticum and Mycoplasma hominis in prostatitis. J. Infect. Dis. 147:807-813.
-
8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of
31/31
THANKYOU