Supported Scaffold Safety An Overview of Scaffold Safety May, 2011.
Structural Evidence for Evolution of the β / α Barrel Scaffold by Gene Duplication and Fusion
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Structural Evidence for Evolution of the β/α Barrel Scaffold by Gene Duplication and Fusion
Horng –uei Lii
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Background
What is β/α Barrel ?
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β/α Barrel (I)
Are found in many proteins(enzymes) The eight-stranded β/α Barrel structure
are one of the largest and most regular of all domain structure
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β/α Barrel(II)
Different structure and function
Loops---varies, functional domain
Evolution material
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TIM barrel
Triosephosphate isomerase
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Introduction Evolution Material Computational Tool Genome Protein Dtata
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Materials
Thermotoga maritimaHisA & HisF
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HisAConverts (ProFAR)
to (PRFAR)
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HisFCatalyze condensation of PRFAR and ammonia,
AICAR and ImGPHisF:HisH cowork
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Methods X-ray analysis Multi-sequence alignment Superimpose Mutual residual activities
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Conclusion
Half-barrel Ancestor Duplication and Fusion Model
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ApplicationSubdomain structures:
Genome searchFunctionally and
structurally related proteins
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Thank you!