Streptavidin-HRP Monoclonal STS antibody

Steroid sulfatase (STS)

J. B. C. 279 (2004) 44704

Biochemistry 2005, 44, 11669-11675

• Tagging an ABP with biotin have two advantages:1. To allows for the visualization of labeling events• by avidin blotting2. the MS characterization of labeling events by enrichmen

t with avidin-conjugated beads.

• biotin moieties remain a central tool for the target identification and certain gel-free profiling platforms,

• protein blotting is a cumbersome and relatively low resolution method for the analysis of probe-labeled proteomes.

Azide Alkyne

Click Chemistry

Proc. Nat. Acad. Sci. U.S.A., 104(8):2614 (2007)