Strawberry DNAExtraction

Plant and

Animal Cells

DNA is found in the Nucleus

From DNA to Organism

Proposed Procedure

As a class, lets come up with a procedure to extract the DNA from the nucleus of the plant cell.

What should we do first?1. First?2. Second?3. Third?4. Fourth?

• SMASH the Strawberry

First Step:Let’s play with our food! Smash the strawberry as best you can WITHOUT breaking the bag.We need to break apart the cell walls to get inside the cell.

Know your Equipment

TEST TUBE

GRADUATED CYLINDER

Funnel

• Add Soap Buffer

Dissolve the MembraneAdd 10ml of extraction buffer to the bag.

Seal the bag and start to smash the strawberry for 1 more min.

We need to dissolve the membrane around the nucleus just like we dissolve the fat from frying pans.

•Filter the Strawberry/Extra

ction MixtureSLOWLY and CAREFULLY pour the mixture through the gauze in the funnel into the test tube.

While letting it drip, draw your extraction set up in the designated box.

We need to strain the cell fragments from the DNA to get a good extraction.

Extraction Set-Up

Funnel

Test Tube

Strawberry Mixture

Paper Towel

•Clump the DNAAdd COLD Ethanol

Now let’s pull the DNA out.Hold out your test tube with the filtered mixture.The teachers will come around and pour COLD ethanol into your test tube.OBSERVE what happens!

Ethanol LayeringDo not mix!