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Stomata An Inquiry Approach. Background 3 Stomata .
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Transcript of Stomata An Inquiry Approach. Background 3 Stomata .
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Stomata
AnInquiry Approach
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Background
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Stomatahttp://www.youtube.com/watch?v=cFX4JrsPaUs
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Skills
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How to: Microscopic Measurement
Determine the diameter of your “field of view.”
1. Begin with a clear ruler.
2. Place on the stage, visible under the lowest magnification for your microscope.
3. Determine the diameter of the field of view in mm.
4. 1mm=1000 Micrometers (μm)
1 mm
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How to: FOV under a higher power lens
For a higher power, you need to calculate the field of view.
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Zoom in
• (Original Obj/New Obj) x Original FOV=New FOV
• FOV=Field of View
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Original Objective
New Objective
Original FOV (μm)
NewFOV
4x 10x 4500 1800
4x 40x 4500 450
4x 100x 4500 180
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Stomata Prints
• Paint a small amount of polish on a small area of leaf
• Let dry• Lay tape on polish and press firmly• Peel off, tape on slide.
• Clear Fingernail Polish
• Clear tape
• Glass Slide
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Stomata Questions
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Onion Osmosis Analysis with Image
J
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Cut a piece of outer pigmented tissue
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Make a wet mount
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Add concentrated salt solution
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Take a picture through the microscope.
Save to computer.
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Determining Percent Change in Area
Find your file and open it.
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To get area and perimeter...
Select the straight line tool. Drag across your field of view.
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Under analyze: Set scale
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Pixels are already there. Enter your known distance.
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Analyze: Set Measurements
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Trace with the freehand toolGo to analyze: Measure
Compare before and after