Stem Cells and their Therapeutic applications
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Transcript of Stem Cells and their Therapeutic applications
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Gold medalist in medicine
Has felicitated by national and International awards for the outstanding work in the field of Regenerative Medicine
Recently felicitated by Ohio State University and was invited for the advanced training in organ development at US
He has several research and scientific publications on his name
A well transplant surgeon in the field of regenerative medicine, treated more than 2000 patients with cellular medicine
Has represented several National and International conference as a chief guest & chief speaker
Member of board of studies in academic programme in ITM university
Achievements
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• Introduction of Stem cells as the building blocks• Stem cells – types• Practical Experience • Future - Drug Discovery in a dish - Organ Shop- fiction or reality? - Organ Development – 3D printing technology - Organ Development – Minimal Invasive Technique - Silicon chip technology• A paradigm shift from traditional medicine to cellular medicine – New
dimensions• Revamping of complete Regenerative Medicine
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1998 - Researchers first extract stem cells from human embryos
1999 - First Successful human transplant of insulin-making cells from cadavers
2001 - President Bush restricts federal funding for embryonic stem-cell research
2002 - Juvenile Diabetes Research Foundation International creates $20 million fund-raising effort to support stem-cell research
2002 - California ok stem cell research
2004 - Harvard researchers grow stem cells from embryos using private funding
2004 - Ballot measure for $3 Billion bond for stem cells
Stem Cell HistoryStem Cell History
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Stem Cell – Definition
A cell that has the ability to continuously divide and differentiate (develop) into various other kind(s)
of cells/tissues
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Stages of Embryogenesis
blastocyst Blastocyst inner mass cells
8-cell stagecleavage
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Blastocyst Diagram
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Embryonic Stem Cells (ESC): received from:
Embryos created in vitro fertilizationAborted embryos
Adult Stem Cells (ASC): can be received from:
Limited tissues (bone marrow, muscle, brain) Discrete populations of adult stem cells generate replacements for
cells that are lost through normal wear and tear, injury or disease.
• IPSC’s reprogrammed cells
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This cellCan form the
Embryo and placenta
This cellCan just form the
embryo
Fully mature
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• Skin • Fat Cells• Bone marrow• Brain• Many other organs
& tissues
Adult Stem CellsAn undifferentiated cells found among specialized or differentiated cells in a tissue or organ after birth
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Bone Marrow
Found in spongy bone where blood cells formUsed to replace damaged or destroyed bone marrow with
healthy bone marrow stem cells.treat patients diagnosed with leukemia, aplastic anemia,
and lymphomasNeed a greater histological immunocompatibility
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Umbilical cord stem cells
Also Known as Wharton’s JellyAdult stem cells of infant originLess invasive than bone marrowGreater compatibilityLess expensive
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Factors known to affect stem cells
Low stress levelsRegular exerciseEnriching experiencesLearning new informationHealthy diets: rich in antioxidantsAvoid excessive drinking
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Dynamic world ; Communication and information
Inside our body their is a microscopic world busy and complex like the world around us. This world is maintained by stem cells
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Signals to Stem Cells
Other Cells
Matrix MoleculesSelf-Renewal
Soluble Factors
Differentiation
Little, et al. Chemical Reviews (2008).
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Health Crisis : Escalating Cost Research Longevity of life
Limitations in treatmentsAuto Immune diseaseOrgan FailuresGenetic diseaseCancer Degenerative / Development / Congenital
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INDIA SPENDS US $ 22.7 BILLION ON HEALTHCARE5.2% of
GDP
Total Healthcare
Pharma Healthcare delivery
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THAILAND INDIA
Heart Surgery
Auto Immune Disease
Organ Failure
Knee Replacement
Cosmetic Surgery
7,500
-------
-------
8,000
3,500
6,000
26,000
69,000
6,000
2,000
40,000
2,50,000
3,00,000
20,000
20,000
UK
23,000
1,50,000
2,00,000
12,000
10,000
Global CostCOST (US$)PROCEDURE
US
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Innovations are required…new treatment therapies are required………..
“We can’t solve the problem by using the same kind of thinking we used when we created them”
- Albert Einstein
Regenerative Medicine
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Tissue Injury
Hemorrhage&
ClottingInflammatory Cell
Infiltrate
Cell Death,Fibroblast Migration,
Debris RemovalNew Host
ECM deposition
ECM Organization& Scarring
TISSUE REPAIR
Rehab
Series of actions & reactions, Proliferation, Differentiation, Migration, Cytokines/ Chemokines & ECM
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Injection in organs directly.
Transplantation of differentiated cells (in vitro) in organs.
Injections in circulation.
Endogenous cells mobilations by repair cytokines , endogenous, growth hormones and growth factors
Heart & brain Very Slow Proliferation Skin & Liver Fast Proliferation
Cellular genetic and nano molecular therapies
Tissue engineering in vitro and in vivo
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Autologous Cellular Therapy for…
Skin burns, skin wounds, ulcers…
Elephantiasis
Orthopedic conditions ( AVN, Arthritis, Bony Fractures and many more)
Diabetes Mellitus and other Autoimmune Conditions
COPD, OCD, Cardiac Ischemia
etc…
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Wound (Before) Wound (After)
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Before Therapy
2nd Week
After Therapy
3rd Week
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Before After
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Ankylosing Spondylitis (After Treatment Results)
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Diabetes Type 1
0.78 3.07
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Osteoarthritis
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After
Before
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1 Orthopedic Conditions ( AVN, OA, Bony fractures, sport injuries)
2 Neuro degenerative and developmental
3 Autoimmune Conditions (MS,AS,RA,DB1, AA etc)
4 Skeleto Muscular (DMD,LGMD, BMD
5 Burns, venous ulcers and non healing wounds
6 Diabetic gangrene wounds
7 Cosmetics
8 Others (Liver Cirrohosis, Elephantiasis, COPD, OCD, ED etc)
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“I have thought a lot about stem cells , and I am sure that when the historians will look back at the 20th century…the greatest scientific achievement will not be from the field of space technology or computer technology but will be from the tissue engineering and genetic medicine.”
-Michael Goulian-ABC Scientific News Reporter,
29-9-1999.
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It takes a tornado to clear theair
Regenerative Medicine in theMidst of the collapsing Health Care
as a tropical storm
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Visualize the future of medicine in next 100 years
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Invention of IPSC’s – Reprogramming of somatic skin cells,
Embryonic Transcription factors : Oct4, Sox2, KLF4,
Cmyc
Developing all 220 types of cells.
“ Its not a science fiction . Its stem cells research led to never before in the history of medical science. Such a gift received to humans – Stem Cells- which can regenerate the cells to tissue to the organs”
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More than 2000 patients treated
One of the leader in therapeutic applications of stem cells
Full equipped facility for sample processing, research and quality control
Trained laboratory and clinical personnel
Conducting PhD/ M.Sc courses in Stem cells in collaboration with ITM
Currently working on several research projects covering mostly clinical disorder in human being
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Academic Collaboration with
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Academic Collaboration with
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Research Collaboration with
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“We live in a time where the words impossible and unsolvable are no longer part of scientific community’s vocabulary. Each day we move closer to trials that will not just minimize the symptoms of diseases and injury but eliminates them”
-Christopher Reeve
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Stem cells therapy & anti ageing center at 7 Hills hospital, Andheri, Mumbai
Stem cells therapy center at Rabale, Navi Mumbai
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Dr.P V Mahajan at AICRACM
discussion with ex-President of India smt. Pratibha patil
discussion with first Ayush cabinet minister Shripad Naik
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in Technical discussion with Luc Montagnier (2008 Nobel laureate)
Dr. P V Mahajan at H3C Conference
with Prof. K Chandan Sen, Executive Director,Ohio State University
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Union Minister Dr. JP Nadda in the conference
Dr. P V Mahajan at H3C Conference
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Dr. P V Mahajan Invited by Ohio State University, USA for Advanced Tissue Culture Training
with Wound healing specialist Dr.Scott Scrape, Director, Transfusion Medicine,
Ohio State University
with Dr.Serber, Regenerative Medicine, Ohio State University
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“ You carry your own repairing kits in your body”