Stem Cells 21 Medical Center

STEMCELLS21: WHO WE ARE

StemCells21 is a Biotech company based in Bangkok, Thailand. The company was established in 2008, founded by Mr. Paul Collier, with the first Regenerative Medicine facility & lab opening its doors in 2011. StemCells21 offers:

• Premium Regenerative Medicine @ IntellliHealth+• Allogenic Stem Cells Supply & Technology• Autologous Stem Cells in-house laboratory services • Product/Protocol R&D: Serum & Essentials

STEMCELLS21: THE COMPANY

IntelliHealth+ centres were established to offer patients stem cell based treatment programs, in 5* modern facilities. When patients undergo a treatment program at IH+, an appropriate stem cell source is selected and a supportive therapy program is designed.

Stem Cell Sources: Umbilical Cord, Peripheral Blood or Adipose.

Supportive Therapies: Peptides/Growth factors, Bio-photon laser treatments, Oxygen therapies and Nutrition/Enzyme/Hormones.

STEMCELLS21: THE FACILITIES

STEMCELLS21: THE FACILITIES

STEMCELLS21: STEM CELLS

What Are Stem Cells?

Stem cells are biological cells that can an self renew to produce more stem cells and differentiate into specialised cell types.

Self Renewal:

Differentiation:



How stem cells work

These cells are capable of performing three important functions with unique abilities:

- Plasticity: Potential to change into other cell types like nerve cells - Homing: To travel to the site of tissue damage or under stimulation- Engraftment: To unite with other tissues

Umbilical Cord-Derived Stem Cells


Corporate Policies: ISO 14001:2004

BSC Safety Audit: British Safety Council

Clinical Research Services: National GLP, GLPCAP (College of American Pathologies)

Regenerative Medicine: AABB (Accredited Cord Blood Facility)

Molecular Medicine: CAP, NABL, CLIA

STEMCELLS21: CELL LAB

Mesenchymal & CD34+ Cells:

Fully characterised, early passage stem cells are available ready for use in cryo-storage.

All products undergo extensive testing including characterisation studies. This gives detailed information on genetic stability, gene expression, and differentiation potential.

STEMCELLS21: MSC & CD34+

Source: human umbilical cord-derived

▪Mesenchymal stem cells are multipotent stem cells that can differentiate into a number of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, neurons, etc.

▪These can have immense applications in regenerative medicine.

STEMCELLS21: MSC

▪Early passage: maximizing stable life-span

▪Spindle morphology consistent with bone marrow MSCs ▪Fully characterised with a panel of cell surface markers

STEMCELLS21: MSC

▪Passage #: P2 or P3 ▪Source: Donated human umbilical cords

▪Microbiological tests: Maternal blood and cord blood tested negative for HIV I&II, HCV, CMV IgG/IgM, syphilis IgG/IgM antibodies and Hepatitis B surface antigen. Free of bacterial and fungal contamination.

▪Morphology: Adherent cells having spindle morphology

▪Representice surface markers: CD31-, vWF- , CD29+, CD44+, CD14-, SSEA4+, HLADR_, HLA ABC+, CD73+, CD105+, CD24-

▪Endotoxin Content by LAL Test <10 EU/ml ▪pH of the Medium 7.4 ±0.2 ▪Average Pre-Freeze Viability >90% ▪Average Purity >90%

STEMCELLS21: MSC

▪Source: human umbilical cord blood-derived

▪CD34 cells are lymphohematopoietic stem and progenitor cells, scaleable up to 10-folds in culture, and capable of maintaining long-term hematopoiesis.

▪They can be used for a variety of purposes including reconstitution of myeloablated marrow, neurological and angiogeneic applications.

STEMCELLS21: CD34+

▪Early passage: maximizing stable life-span

▪Good morphology: non-adherent,translucent and round cells

▪Fully characterised with a panel of cell surface markers

STEMCELLS21: CD34+

▪Passage #: P1 or P2 ▪Source: Human umbilical cord blood of donated umbilical cords.

▪Microbiological tests: Maternal blood and cord blood tested negative for HIV I&II, HCV, CMV IgG/IgM, syphilis IgG/IgM antibodies and Hepatitis B surface antigen. Free of bacterial and fungal contamination.

▪Morphology: Non adherent translucent round cells

▪Representive surface markers: CD33+, CD38+, CD34+

▪Endotoxin Content by LAL Test <10 EU/ml ▪pH of the Medium 7.4 ±0.2 ▪Average Pre-Freeze Viability >90% ▪Average Purity >90%

STEMCELLS21: CD34+

Autologous Adipose Tissue-derived Stromal Vascular Fraction

STEMCELLS21: ADIPOSE SVF

How it Works:Our technology process yields SVF which is a functionally diverse population of cells that is believed to be synergistic and able to communicate with other cells in their local environment.

Ultrasonic Cavitation Separation process is:• Clean/Safe – no animal products or

enzymes • Efficient– complete time 30 - 40

minutes with cell counting• High number of SVF• Extra cellular matrix present which

aids in the regenerative process• Can be applied to a variety of

diseases



Prior to our process, the cell membrane's are clearly defined

Contents of the Adipose cell begin to emulsify.

Emulsifications of the fats and water content to occur.

Homogenization of the Adipose tissue has occurred!

▪Derived from Blood Vessels in adipose (fat) tissue ▪Capillaries are primary source (see inset below) ▪Same-day clinical procedure ▪Easy to harvest fat ▪High cell counts

Blood vessels

Adipose (fat) cells

Capillaries

The isolated SVF contains:▪ Adult Stem Cells

▪ Endothelial Cells

▪ Fibroblast Cells

▪ Pericytes

▪ Red Blood Cells

▪ White Blood Cells

… and much more


Varma et al, Stem Cells and Development 2007:91-104 (freshly isolated adipose). Repeated by Collas et al and Yoshimura et al.

CD90+ positive cells after ultrasonic cavitation


Fraser JK, Wulur I. Fat tissue: an underappreciated source of stem cells for biotechnology. Trends in Biotechnology. 2006;24:150-154.

Dapi staining of lipoaspirate. Dapi nuclear stain highlights the capillaries containing MSC’s


Extracellular matrix (ECM) • Basement membrane (type 4 reticular collagen) • Interstitial matrix (Type 1 collagen)

ECM regulates cell function and is a reservoir of growth factors. Triggering protease activity releases growth factors

ECM controls growth, wound healing and fibrosis; is able to block the immune reaction of inflammation and scarring to allow replacement tissue to grow in the absence of scarring.


0.4-1 Million Cells per gram of adipose

15–30 % Dead Cells

Digests ECM

More adipose required (more time)

Animal or bacterial product

8-16 Million Cells per gram of adipose

5-15 % Dead Cells

Preserves ECM

Less adipose required (less time)

Clean and safe

Ultrasonic Cavitation Proprietary Technology

! Collagenase Enzyme


Autologous EPCs & APCs:

Endothelial Progenitor Cells & Angiogenic Precursor Cells:

Prepared from patients whole blood cultured for specific cell type EPCs & APCs for regeneration of vascular.

Key areas of treatment: Vascular disease

Immunotherapy Cell Therapies:

Autologous natural killer cells (NK), Cytokine induced killer cells (CIK) & Dendritic (DC) vaccines derived from the Patients blood. Delivered in multiple infusion programs.

Key areas of treatment: Cancers, Viral Infections and Immune system disorders.

Fibroblasts:

1) Autologous cultured fibroblasts for wound healing and cosmetic skin rejuvenation

2) Neo-natal ready available fibroblasts for wound healing and cosmetic skin rejuvenation

Plasma-Derived:

PRP preparations for a variety of conditions: Skin Rejuvenation, Orthopaedic, Muskuloskelatal Pain

Stem Cells 21 Medical Center

Health & Medicine

Transcript of Stem Cells 21 Medical Center