ANTI-OXIDANT ACTIVITY

Rich in substances with antioxidant activity higherthan that of common natural antioxidant benchmarks

STEM CELL TECHNOLOGY

7130 Seacrest Blvd, Palm Beach, FL 33462 • 1-800-796-7546 • www.imageskincare.com

Stem extracts and Phytonutrients

IMAGE SKINCARE

ANTI-COLLAGENASIC ACTIVITY

Inhibits collagenase thus maintaining skin firmness and limiting collagen degradation

EPIDERMAL STEM CELLS IN THE HUMAN SKIN

Stratum corneum

Differentiating progeny of one epidermal stem cell

Granular layer

Spinous layer

Basal layer

Dermis

Transient amplifying cell

Epidermal stem cell

Basementmembrane

Up-Regulation of Specific Genes involved in the Delay of Senescence

Genes after H2O2

Controlafter H2O2

+ 2 % Malus Domesticastem cell extract

cyclin B1: induces proliferation 73 130

cyclin E1: cell cycle regulator 78 135

p53: tumor suppressor gene 63 137

insulin-like growth factor II: cell proliferation enhancer 71 117

heme oxigenase 1: antioxidant enzyme 89 211

Gene, that is down regulated by senescence induction (Control)Gene, that is up regulated or normalized in senescent cells by PhytoCellTec™ Malus Domestica

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1 * Malus domestica fruit cell cultureOrigin:Stem cells of Spaetlauber apple tree from Switzerland, excellent storability, rich in phytonutrients, proteins and long-living cellsFunctions: • protects longevity of skin stem cells• combats chronological aging• protection against oxidative stress• repair of DNA

2 Vitis vinifera fruit cell cultureOrigin:stem cells of rare red-flesh grape cultivar, contains epigenetic factors and metabolites that are able to protect human skin stem cellsFunctions: • protects epidermal stem cells against UV damage• fights photo aging

3* Leontopodium alpinum meristem cell cultureOrigin:alpine Edelweiss flower that developed defense mecha-nisms to survive extreme climatic and environmental conditionsFunctions: • anti-inflammatory• high in anti-oxidant capacity (high TEAC factor, trolox equivalent anti-oxidant capacity)

4 Argania spinosa callus tissueOrigin:ancient barber tree from Marocco that belongs to oldest tree species of the world, able to resist extreme droughts and extremely high temperaturesFunctions: • proven effect on dermal stem cells• vitalizes dermal stem cells, which are responsible for collagen and elastin production• fights wrinkles and loss of firmness

5 Leucojum aestivum bulb extractOrigin: Summer snowflake flower, survives in extreme climatesFunction:• slows down cell proliferation, aging process• youth preservation• BOTOX like effect on muscle contraction

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7130 Seacrest Blvd, Palm Beach, FL 33462 • 1-800-796-7546 • www.imageskincare.com

Cells are grown from active Vigna radiata meristematic tissues

Vacuole

Mitochondria

Chloroplasts

Nucleus

Membrane

Rescue of UV-damaged skin fibroblastswith Plant C-Stem™ Vigna radiata

The addition of Plant C-Stem™ Vigna radiata increased cell viability by 2.3-fold

05

20

40

60

80

100

10 15 20 25 30 35 40 45 50

Cel

l via

bilit

y (%

MTT

)

Time (hour)

38%

88%

Fibroblasts + Plant C-Stem™ Vigna radiata

Plant C-Stem™ Vigna radiata

Control fibroblasts

Cell viability 2.3

UVB (50 mJ/cm2)

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6* Phaseolus radiata meristem cell culture extractOrigin:Vigna radiata, mung bean sprouts, selective extraction of the vacuolar content, plant is capable of protecting against various environmental stressorsFunctions: • decrease of pro-inflammatory mediatorsa and extra cellular matrix degradation enzymes• increase in collagen• rescue of UV damaged fibroblasts: UV damage repair, reduced photodamage

7 Echinacea angustifolia stem cellsOrigin:Herbaceous plant, stem cells are extracted from buds or root tips, these undifferentiated cells are activated to produce bioactives, EchinacosidesFunctions: • Fades dark circles• reduces capillary permeability, by nitric oxide reduction • reinforces the capillary wall• strengthens thin and delicate skin, stimulates collagen synthesis

8 Sulfora WhiteOrigin:Liposomal preparation of swiss garden cress sprouts rich in sulforaphane, a powerful antioxidant phytonutrientFunctions: • effectively inhibits melanin production• provides brightening benefits• fades the appearance of dark spots and discolorations

9 Opunthia ficus-indica stem extractOrigin:Ultra refined elixir from organic paddle cactusFunctions: • blocks release of stress markers• soothes and firms irritated skin

10 Epilobium angustifolium stem extractOrigin:Canadian Willowherb is a natural plant from the Northern Canadian prairies which has developed strong multi-func-tional phytochemical properties to survive the harsh climate. The plant extract contains bioactive moleculesFunctions: • free radical scavenger• reduces erythema caused by UV radiation

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