Stem Cell Research Solutions - Thermo Fisher …...Stem cell research requires cellular and...

Stem Cell Research SolutionsPromotional offers

CULTUREENGINEERINGDIFFERENTIATIONCHARACTERIZATION

2 Life Technologies™ | Stem Cell Research Solutions

For more than a decade, we have provided you with key tools to address challenges in stem cell research. Now we’ve harnessed the power of these innovations to offer a total system covering the entire research spectrum. With our complete portfolio of stem cell products and services, you can benefit from the continuity, efficiency, and precision that drives discovery. With Gibco® stem cell culture products, a broad Invitrogen™ portfolio of stem cell differentiation and engineering products, and a wide array of stem cell analysis tools that include Molecular Probes® labeling and detection reagents, we have the products to support you at every step of your research.

Stem Cell research products


SPECIAL OFFERS*

Order 2 qualifying products and receive a 10% discount.

Order 3 or more qualifying products and receive a 20% discount.

Quote promo code RLNS02 when ordering.

Offers expire December 3, 2014. *Terms and conditions apply. See back page for complete details.

OFFER 2

OFFER 1

3Life Technologies™ | Stem Cell Research Solutions

Pluripotent Stem Cells (PSCs)

KnockOut ™ Serum Replacement Gold for -ESC and iPSC cultures

KnockOut™ Serum Replacement (KnockOut™ SR) is a defined, serum-free formulation used to support the growth of undifferentiated ES and iPS in culture. ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least. The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture.

Feeder free, Serum free Induced Pluripotent Stem Cell Culture

Essential 8™ Medium, originally developed in the laboratory of James Thomson, contains the 8 essential components needed for pluripotent stem cell culture. Essential 8™ Medium enables streamlined expansion of PSCs. Essential 6™ Medium is the same formulation as Essential 8™ Medium, but does not contain TGF-ϐ or bFGF and enables reprogramming and embryoid body formation/differentiation.

StemPro® EZPassage™ Disposable Stem Cell Passaging Tool

The StemPro® EZPassage™ Disposable Stem Cell Passaging Tool is a novel human embryonic stem cell passaging device that makes manual passaging of stem cell colonies more rapid and reproducible.

Product Size Catalog Number

Essential 8™ Medium 500 mL A1517001Vitronectin (VTN-N) Recombinant Human Protein, Truncated 1 mL A14700KnockOut™ Serum Replacement 500 mL 10828028StemPro® hESC SFM 1 kit A1000701KnockOut™ D-MEM CTS™ 500 mL A1286101CTS™ KnockOut™ DMEM/F-12 500 mL A1370801KnockOut™ D-MEM 500 mL 10829018KnockOut™ DMEM/F-12 500 mL 12660012KnockOut™ SR XenoFree CTS™ 100 mL 12618012KnockOut™ SR XenoFree CTS™ 500 mL 12618013Essential 6™ Medium 500 mL A1516401StemPro® EZPassage™ Disposable Stem Cell Passaging Tool 10 units 23181010Geltrex® LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix 1 mL A1413301

Geltrex® hESC-qualified Ready-To-Use 50 mL A1569601

1st operation

Rotate the dish90° and roll again

2nd operation

MediumCells

Learn more about our stem cell products and resources at lifetechnologies.com/stemcells


iPSC Reprogramming Tools

CytoTune®–iPS 2.0 Sendai Reprogramming Kit

The CytoTune®-iPS 2.0 kit utilizes a non-integrating Sendai virusparticles to deliver the Yamanaka factors and is extremely easy to use, requiring only one overnight incubation compared to the multiple days of transductions required for mRNA reprogramming. Sendai reprogramming vectors do not integrate into chromosomes of the target cells and therefore do notpotentially disrupt important genes. They are capable of transducing a wide range of cell types such as BJ fibroblasts, PBMCs, and CD34+ blood cells.

Epi5™ Episomal iPSC Reprogramming Kit

The Epi5™ Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University. This system produces transgene-free, virus-free human induced pluripotent stem cells (iPSCs) with efficiencies in the range of 0.04% to 0.3%, depending upon the cell type being reprogrammed. Lipofectamine® 3000 reagent with the Epi5™ Episomal iPSC Reprogramming Kit enables highly efficient reprogramming of somatic cells without the need for electroporation.


CytoTune® 2.0 -iPS Sendai Reprogramming Kit (1 Pack) 1 pack A16517CytoTune® 2.0-iPS Sendai Reprogramming Kit (3 Pack) 3 pack A16518Epi5™ Episomal iPSC Reprogramming Kit 1 kit A15960


Lipofectamine® 3000 Transfection Reagent 0.75 mL L3000008Lipofectamine® 3000 Transfection Reagent 1.5 mL L3000015Lipofectamine® 3000 Transfection Reagent 5x1.5 mL L3000075Lipofectamine® 3000 Transfection Reagent 15 mL L3000150


Mesenchymal Stem Cells (MSCs)


StemPro® BM Mesenchymal Stem Cells 1 x 106 cells A15652StemPro® Human Adipose-Derived Stem Cell Kit 1 kit R7788110StemPro® Human Adipose-Derived Stem Cells 1 vial R7788115MesenPRO RS™ Medium (NZ) 1 kit E071000CTS™ StemPro® MSC SFM 1 kit A1033201StemPro® MSC SFM XenoFree 1 kit A1067501StemPro® Adipogenesis Differentiation Kit 1 kit A1007001StemPro® Chondrogenesis Differentiation Kit 1 kit A1007101StemPro® Osteogenesis Differentiation Kit 1 kit A1007201

Gibco® media include the broadest selection of specialty products optimized for mesenchymal stem cell populations, many of which are free of animal-derived components. These media are designed to minimize adaptation time, maximize cell performance, and help meet regulatory requirements.

StemPro® NSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human neural stem cells (NSCs). It enables superior human NSC expansion of both adherent and spheroid cultures derived from human embryonic stem cells or fetal tissue compared to existing commercial products. StemPro® NSC SFM also maintains the multipotential differentiation capability into astrocytes, oligodendrocytes and neurons.

Life Technologies™ provides a comprehensive set of tools for isolation, expansion, differentiation, and characterization of HSCs and their derivatives. CD34 is a common marker for identifying human HSCs, and Life Technologies™ offers a serum-free medium for CD34+ cells, as well as methods to isolate CD34+ cells from blood or bone marrow.

Neuronal Stem Cells (NSCs)

Hematopoietic Stem Cells (HSCs)


Neurobasal® Medium (1X) Minus Phenol Red 500 mL 12348017Neurobasal® Medium (1X), Liquid 500 mL 21103049Neurobasal®-A Medium (1X) Minus Phenol Red 500 mL 12349015Neurobasal®-A Medium (1X), Liquid 500 mL 10888022StemPro® NSC SFM 1 kit A1050901 B-27® Serum-Free Supplement (50X), Liquid 10 mL 17504044B-27® Supplement Minus AO (50X), Liquid 10 mL 10889038B-27® Supplement Minus Vitamin A (50X), Liquid 10 mL 12587010G-5 Supplement (100X), Liquid 1 mL 17503012


StemPro® CD34+ Cell Kit 0.5 x 106 cells⁄mL A14059StemPro®-34 SFM (1X), Liquid 1 kit 10639011



Coating reagentsProduct Size Catalog Number

Fibronectin Human, Plasma 5 mg 33016015Fibronectin, Bovine Plasma 1 mg 33010018CELLstart™ CTS™ 2 mL A1014201Natural Mouse Laminin 1 mg 23017015

Stem cell research requires cellular and molecular tools to confirm pluripotency or to help determine the utility of cells in downstream experiments. Whether analyzing proliferation, protein levels, gene expression, or epigenetic profiles, Life Technologies™ has the right instruments, products, and services for your research.

Dissociation reagents

Characterization


Dispase 5 g 17105041StemPro® Accutase® Cell Dissociation Reagent 100 mL A1110501UltraPure™ 0.5M EDTA, pH 8.0 4x100 mL 15575020TrypLE™ Select CTS™ 100 mL A1285901


Alkaline Phosphatase Live Stain 50 µL A14353AlexaFluor® 488 CD44 50 tests A25528AlexaFluor® 488 Tra-1-60 50 tests A25618AlexaFluor® 555 Tra-1-60 50 tests A24879AlexaFluor® 594 Tra-1-60 50 tests A24882SSEA4 Mouse Anti-Human mAb (clone eBioMC-813-70 (MC-813-70)), Alexa Fluor® 488 25 tests A14810SSEA4 Mouse Anti-Human mAb (clone MC813-70), Alexa Fluor® 647 Conjugate 0.5 mL SSEA421SSEA4 Mouse Anti-Human mAb (clone eBioMC-813-70 (MC-813-70)), PE Conjugate 50 µg A14766CD44 Rat Anti-Human/Mouse mAb (clone IM7) Fluorescein (FITC) Conjugate 50 µg A16193Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit 40 tests A24881Pluripotent Stem Cell Immunocytochemistry Kit (Sox2, Tra-1-60) 40 tests A25525Pluripotent Stem Cell Immunocytochemistry Kit (Oct4, SSEA4) 40 tests A25526Human Neural Stem Cell Immunocytochemistry Kit 20 tests A24354Human Cardiomyocyte Immunocytochemistry Kit 50 tests A25973TaqMan® hPSC Scorecard™ Panel, 96-well fast (1 kit) 2 x 96 well plates A15871TaqMan® hPSC Scorecard™ Panel, 96-well fast (1 panel) 2 x 96 well plates A15876TaqMan® hPSC Scorecard™ Panel, 384-well (1 kit) 1 x 384 well plates A15872TaqMan® hPSC Scorecard™ Panel, 384-well (1 panel) 1 x 384 well plates A15870


Gibco® media and supplements for PSC differentiation provide robust, physiologically relevant results with simple protocols. These innovative tools are designed to simplify and speed up complex protocols for differentiating PSCs in to different cells types so you can spend more time focusing on the outcomes of your research.

Differentiation


Gibco® PSC Neural Induction Medium 1 kit A1647801PSC Cardiomyocyte Differentiation Kit (prototype) 1 kit A25042SA

Achieve better biology in your stem cell researchLipofectamine® 3000 ReagentGeneration and transfection of induced pluripotent stem cells (iPSCs).

Learn more at lifetechnologies.com/3000





iPSCs

Ectoderm

Mesoderm

EndodermReprogramming

factors

Adult cells

Indel in genomic DNA

Lipofectamine® 3000 reagent with the Epi5™ Episomal iPSC Reprogramming Kit enables highly efficient reprogramming of somatic cells without the need for electroporation.

Lipofectamine® 3000 transfection reagent also improves the cleavage efficiency with CRISPR vectors on pluripotent stem cells, yielding a 2-fold increase in the efficiency of genetic modifications.

Lipofectamine® 3000 reagent can deliver DNA into ESCs and iPSCs in culture.

Lipofectamine® 3000 reagent can deliver DNA into difficult-to-transfect differentiated cells in culture.

*Terms and conditions: This promotion is open only to customers in Australia, India, New Zealand and Singapore who purchase two or more qualifying products in a single purchase order during the promotional period. The list of qualifying products include all product catalog numbers listed throughout this brochure. Offers will apply to eligible orders received by Life Technologies no later than December 3, 2014 or until promotional supplies are depleted, whichever comes first. Discount applies to the list price in effect at the time the order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

For Research Use Only. Not for use in diagnostic procedures. © 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. CytoTune is a trademark of DNAVEC Corporation. Essential 8™ & Essential 6™ are trademarks of Cellular Dynamics International, Inc. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. C0240566 IPAC 1014

CytoTune®-iPS 2.0 Sendai Reprogramming KitThe highest reprogramming efficiency for iPS cell generation

Learn more at lifetechnologies.com/cytotune

Stem Cell Research Solutions - Thermo Fisher …...Stem cell research requires cellular and...

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