Status Report DNA Barcoding in Argentina
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Transcript of Status Report DNA Barcoding in Argentina
Status ReportDNA Barcoding in Argentina
Pablo Luis TubaroMuseo Argentino de Ciencias Naturales “Bernardino Rivadavia”
iBOL Argentina Fund
Five calls for projects from 2008 to 2012, 110 projects supported.
The structure of the iBOL project in ArgentinaiBOL Argentina Committee
Five Reference Barcode Labs.
Leading Labs Training Workshops for DNA Barcoding.
iBOL coordinator
Cruises of the Oceanographic vessel
Structure of Argentinian barcoding network
MACN – Buenos Aires
Nágera Biological Station Mar del Plata
INIBIOMA - Bariloche
CENPAT – Puerto Madryn
IBONE - Corrientes
How Barcode Network works in Argentina
Projects supported byiBOL Argentina Fund
Projects supported byiBOL Argentina Fund
BIO/CCDBLysis plates
PCR Plates (no permits required)
Exporting permits (Fauna, Acceso Rec. Genéticos 226/2010)
Five ReferenceBarcode LabsCONICETLlabs. Training Workshops atMACN
Barcodes
Projects supported byiBOL Argentina Fund
Building the DNA barcode library of Argentinian species
The projects supported by iBOL Argentina Fund
• The researchers must be principal investigators of a PIP or a PICT.
• Tissues and vouchers must be stored in permanent collections.
• Tissues and vouchers must be compliant with barcode standards.
• The funds support only the extra costs of preserving tissues and vouchers, including small equipments, technical services, etc. Only small amounts are allowed to support field work. No PCR and sequencing costs are supported. • Researchers and their fellows have to take the Leading Labs Training Workshop for DNA barcoding.
• Specimens must be collected following legal norms.
• Researchers have to send the tissue samples to CONICET – iBOL Labs. to produce their barcode sequences.
Leading Labs Training Workshop for DNA Barcoding - MACN
“Puerto Deseado” cruises to get samples for iBOL projects
The iBOL-IDRC project
Objetives:
Engaging Developing Nations in the International Barode of Life Project (iBOL)
• To support some key elements of the iBOL project structure.
• To create barcode libraries that allow practical applications in the participating
countries.• To secure the active participation of five developing nations in the iBOL project.
• To develop an ABS policy for the iBOL project.
ActinopterygiiUnited States 12810 Argentina 2184 China 1364Australia 9033 Philippines 2130 Panama 1295Mexico 6527 India 2048 Malaysia 1166Brazil 6444 Taiwan 1680 Madagascar 885Canada 6292 Kenya 1642 New Caledonia 747Indonesia 3520 France 1607 Seychelles 740South Africa 2238 French Polynesia 1524
128.914 barcodes belonging to 13.183 species
Canada 4992 Brazil 1741 Bolivia 555Peru 3491 Japan 1399 Australia 538Argentina 3195 Indonesia 1109 Sweden 449Panama 2046 Papua New Guinea 695 Netherlands 448United States 1873 New Zealand 642 Philippines 336Mexico 1788 Norway 631 Spain 318Russia 1756 Ecuador 612
Aves 32030 barcodes belonging to 4563 species
Costa Rica 157609 Germany 12133 South Africa 6400Australia 106719 China 10924 Panama 5823Canada 98213 Brazil 10141 Argentina 5466United States 90582 Peru 9807 Finland 4519Papua New Guinea 19524 Indonesia 8028 United Kingdom 4295Mexico 15256 Italy 7380 Russia 4287Ecuador 12961 Spain 7141
Lepidoptera 714.950 barcodes belonging to 73.377 species
Canada 2565 Thailand 463 Panama 160Costa Rica 1583 Brazil 453 Colombia 121United States 1459 Bolivia 266 India 114Argentina 743 South Africa 239 Peru 111Kenya 688 Guatemala 232 Chile 104Germany 678 Russia 230 Norway 99Mexico 613 Australia 185
Apidae 12.311 barcodes belonging to 2021 species
Barcode Argentine Butterflies (ARLEP) and Moths (ARMOT)
ARMOT
Emerging Leaders in the Americas Program - ELAP
PLO0308AAA9670
11.7% COIdivergence
4.7% COIdivergence
12.2 COIdivergence
PLO0104ACA8897
PLO0104AAA9669
Finding a deletion
Building the Biodiversity Macroscope in Bolivia
CIAR – Pcía de Misiones – Selva subtropical
Ea. El Bagual – Pcia de Formosa – Chaco húmedo
Malaise 1: 27.44476ºS 54.94032ºOMalaise 2: 27.44300ºS 54.94237ªO 139 msnm
EQP-CLL-513 26 18’10.0” S 58 48’54.0”W 57 msnmEQP-CLL-512 26 18’11.1” S 58 48’55.8”W 56 msnm
Global MalaiseProgram
Future plans (next 12 months)
• To continue working in collaboration with Bolivia to build its Biodiversity macroscope, overcoming its new regulations.
• To increase the production of COI products in CONICET’s Barcode Labs.
• To expand the Global Malaise Trap Program in protected areas in Argentina and Bolivia and set their sample processing pipelines.
• To help other Latin American countries in barcode projects – e.g. Peru, Dominican Republic, etc.
• To open a new call of projects for the iBOL Argentina Fund and to organize the Sixth LLABs training workshop for DNA barcoding.
Secured
Secured
Secured
Next trip to Bolivia postponed given current restrictions; additional support required to continue after 2013
Working on both aspects, additional funds required
Next biota?The Biodiversity of the Iguazú
National Park - Argentina
The Iguazú National Park (in Argentina) and the Iguaçu National Park (in Brazil) represent the southern tip of the Atlantic forest, and together with the preserves of Misiones Province are the largest and best preserved remains of this ecosystem.
The Atlantic forest, one of the richest and more endangered ecosystems of the World
Objectives1) To discover which species are within Iguazú National Park.2) To build a DNA barcoding database that will permit the identification of the
species of Iguazú National Park in a cost-effective and rapid manner.3) To describe the new species that will result from sampling the fauna of Iguazú
National Park.4) To provide a biodiversity data baseline for the Iguazú National Park that will be a
benchmark against which future biodiversity studies can be compared.