st International Conference on Tropical Wetland ...

1st International Conference on Tropical Wetland Biodiversity and Conservation (ICWEB)

1


2

Contents

Cover 1 Contents 2 Organizing Committee’s Welcoming Speech 3 ICWEB 2019 Committee 4 Program Schedule 5 Invited Speaker: 13 1. Dr. Karmele Llano Sánchez, International Animal Rescue Indonesia, Ketapang,

West Kalimantan, Indonesia 14

2. Dr. Matthew Hayward, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia

15

3. Dr. Reuben Sunil Kumar Sharma, Centre for Wildlife Research and Conservation, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Malaysia

16

4. Prof. Johan Michaux, Gecolab Laboratory, Institute of Botany (Bat. 22). University of Liège, Belgium

17

5. Amalia Rezeki, S.Pd., M.Pd., Sahabat Bekantan Indonesia 18 6. Dr. Drs. Krisdianto, M.Sc., Biology, Faculty of Mathematics and Natural Sciences,

Univeritas Lambung Mangkurat 19

7. Dr. drh. Hery Wijayanto, M.P, Faculty of Veterinary Medicine, Universitas Gadjah Mada

20

8. Prof. Dr. Ir. H. Abdul Hadi, M.Agr., Soil Science, Faculty of Agriculture, Universitas Lambung Mangkurat

21

Abstracts Oral Presentation 22 1. Conservation and Management 23 2. Sustainable Tourism 32 3. Wetland Ecosystem 37 4. Wetland Plant and Microbes Biodiversity 43 5. Wetland Biodiversity 55 6. Wildlife Biology and Medicine 70 Abstracts Poster Presentation 83


3

Organizing Committee’s Welcoming Speech

Dear Colleagues and Participants, It is a great pleasure to welcome colleagues and participants from around the world to the 1st International Conference on Tropical Wetland Biodiversity and Conservation (CWEB) that is held in Banjarbaru, South Kalimantan, Indonesia from November 1-2, 2019. This conference is hosted by Department of Biology, Faculty of Mathematics and Natural Sciences, Lambung Mangkurat University, Banjarbaru, in collaboration with Faculty of Veterinery Gadjah Mada University, Sahabat Bekantan Indonesia and Balai Konservasi Sumber Daya Alam (Nature Conservation Agency of South Kalimantan). The theme of the conference is “Maintaining the tropical wetland biodiversity for better human welfare”. This event relates to an integrated global perspective for a map out the various drivers and alternative scenarios as an integrative solution for an update tropical wetland biodiversity and conservation issues. The conference provides an ideal platform to share information and discuss their scientific results and experiences, with particular references to maintain tropical wetland initiative. Banjarbaru, is a fast growing city in the province of South Kalimantan, Indonesia and famously known as an urban city with a unique natural landscape, a cultural diversity, and a friendly welcoming citizen. Moreover, Banjarbaru will become the centre of provincial government that its government is located in Banjarmasin today. I can guarantee you that this conference provides a full of intellectual experience as well as an unforgettable visit to Banjarbaru. We look forward to welcoming you on our place. Yours sincerely, Chairperson, Sasi Gendro Sari


4

ICWEB 2019 Commitee

STEERING COMMITTEE

Condescendent

• South-Kalimantan Governoor

• Lambung Mangkurat University Rector

• Gadjah Mada University Rector

Advisors

• Mathematics and Science Faculty Dean of Lambung Mangkurat University

• Faculty of Veterinary Medicine Dean of Gadjah Mada University

• Head of Natural Resources Conservation Agency of South Kalimantan Province

• Founder of " Sahabat Bekantan Indonesia" Foundation

ORGANIZING COMMITTE

Chairman : Sasi Gendro Sari, S.Si., M.Sc.

Vise of Chairman : Amalia Rezeki

Secretary : Rani Sasmita, S.Si., M.P., M.Sc.

Exchequer : Dhian Asmarini, S.Si

1. Transportation and Tourism Section

• Anang Kadarsah, S.Si., M.Si.

• Zainal Abidin

2. General Equipment Section

• Dr. Dindin H. Mursyidin, M.Sc.

3. Secretariat, Proceeding and Abstract Editor

• Dr. Ir. Badruzsaufari, M.Sc.

4. Secretariat, Registration Section

• Witiyasti Imaningsih, S.Si, M.Si

5. Secretariat, Proceeding Section

• Irma Padeta

• Ambar Pertiwi

• Zainudin

• Dr. Dindin H. Mursyidin, M.Sc.

6. Publication and Documentation Section

• Ahmad Rusadi, S. Kom., M.Kom.

• Rudy Herteno, S. Kom., M.Kom.

• Zainal Abidin

7. Event Division

• Hasrul Satria Nur, S.Si., M.Si.

• Irma Padeta

• Titik Sundari

• Yasmin Qamarani

8. Consumption Division

• Dr. Dra. Rusmiati, M.Si.

9. Enterprise and Sponsorship

• Enterprise and Sponsorship

• Heri

• Helda


5

STUDENT COMMITTEE

1. Event Division

• Mohamad Ardiansyah (Coordinator)

• Mas'arisaldy Khairul Barkatullah

• Evita Novetsyeba Mosooli

• Alya Nur Afifah

• Nie’mah Al’as

• Yuninda Riyana

• Gusti Maharani

• Era Khoridatul Badiatuz Zahro

• Ahmad Najmi Aulia

2. Secreat

• Nabila (Coordinator)

• Ayna Maulannur (Coordinator)

• Rusyda Ulya

• Selly Ariska Putri

• Della Adventaria

• Nadiya Dwi Rahayu

• Noveani Annisa Rusalinda Rahmi

• Nurul Huda

3. General Equipment Section

• Aldy Hernanda (Coordinator)

• Nico Allianur

• Sapta Budiarto

• Muhammad Erwansyah

• Ahmad Winarto Saputra

• Riduansyah Syamsudin

4. Transportation and Tourism Section

• Ridho Dinta Masmitra (Coordinator/Driver)

• Msy Elsa Mayori Aurora (Driver)

• Mas'arisaldy Khairul Barkatullah (Tour Guide)

• Evita Novetsyeba Mosooli (Tour Guide)

• Alya Nur Afifah (Tour Guide)

• Gusti Nur Aida Fasha (Tour Guide)

5. Publication and Documentation

• Normeilawati (Coordinator)

• Muhammad Rizqan Fadillah

• Muhammad Fitri

6. Consumption

• Nor Azizah (Coordinator)

• Rahimah (Coordinator)


6

CONFERENCE PROGRAM

Friday: November 1, 2019

07.00-08.00 Registration and Panting Music

08.00-08.15 Welcoming Ceremony: Baksa Kembang

08.15-08.20 Indonesian National Anthem and Opening Prayer

08.20-08.45 Opening Speech and Ceremony 1. Dean, Faculty of Mathematics and Natural Sciences, Universitas Lambung Mangkurat. 2. Dean, Faculty of Veterinary Medicine, Universitas Gadjah Mada. 3. Vice Rector, Planning, Cooperation and Public Relations, Universitas Lambung Mangkurat

08.45-09.30 Speech and Opening Seminar

Governoor of South-Kalimantan

H. Sahbirin Noor, S.Sos., M.H.

09.30-09.45 Photo Session

09.45-10.00 Coffee break

10.00-11.00 Keynote Speech: 1. Vice Rector, Planning, Cooperation and Public Relations, Universitas Lambung Mangkurat:

Prof. Dr. Ir. H. Yudi Firmanul Arifin, M.Sc. 2. Direktur Bina Pengelolaan Ekosistem Esensial Kementrian Lingkungan Hidup: Ir. Tandya

Tjahjana, M.Si Moderator: Dr. Ir. Mahrus Arydi, M.Sc.

11.00-12.00 Invited Speaker 1 Invited Speaker 2

Dr. Karmele Liano Sánchez, Veterinary Medicine Spain, Animal Rescue Indonesia, Ketapang, West Kalimantan, Indonesia Importance of Tropical Wetlands in the Conservation of Orangutans (Pongo pygmaeus)

Dr. Matthew Hayward, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia Conservation of Wetland Dependent Fauna – Lessons Learned from Case Studies of the Quokka Setonix brachyurus, European bison Bison bonasus, European beaver Castor castor, and Australian Arid Zone Fauna

Moderator: Drh. Teguh Budipitojo, MP., Ph.D.

12.00-14.00 Poster Session and Lunch

14.00-15.00 Invited Speaker 3 Invited Speaker 4

Prof. Johan Michaux, Gecolab Laboratory, Institute of botany (Bat. 22). University of Liège, Belgium The Revolution of New Genetic Tools for the Study of the Ecology of Rare or Elusive Species, using Non-Invasive Approaches

Dr. Reuben Sunil Kumar Sharma, Centre for Wildlife Research and Conservation, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Malaysia Urban Wetland Ecosystems: Sustaining Biodiversity and Enhancing Water Quality

Moderator: Dr. Ir. Badruzsaufari, M.Sc.

15.00-15.30 Coffee Break and Ashar Prayer

15.30-16.00 Invited Speaker 5, 6, 7, and 8:

Room A Room B Room C Room D

Amalia Rezeki, Sahabat Bekantan Indonesia The Role of Sahabat Bekantan Indonesia in Conservation of Bekantan in Indonesia

Dr. Drs. Krisdianto, M.Sc., Biology, Faculty of Mathematics and Natural Sciences, Univeritas Lambung Mangkurat How the local people face the rapid change of the tropical wetland environment?

Dr. drh. Hery Wijayanto, M.P, Faculty of Veterinary Medicine, Universitas Gadjah Mada The Big Nose of Bekantan (Nasalis larvatus)

Prof. Dr. Ir. H. Abdul Hadi, M.Agr., Soil Science, Faculty of Agriculture, Universitas Lambung Mangkurat Response of Peat Soil’s Microbes to Drainage and Forest Fire

Moderator: Sunardi, S.Si., M.Sc., Ph.D.

Moderator: Dr. drh. Aris Haryanto, MP

Moderator: Mrs. Irma Padeta

Moderator: Hasrul Satria Nur, S.Si., M.Si.

16.00-18.00 Parallel: Oral session

Room A: Conservation and Management (CM); Sustainable Tourism (ST)


7

Room B: Wetland Ecosystems (WE); Wildlife Biodiversity (WB)

Room C: Wildlife Biology and Medicine (WBM)

Room D: Wetland Plant and Microbes Biodiversity (WPMB)

18.00-18.30 Closing ceremony

FIELD TRIP

Saturday: November 2, 2019

04.45-05.00 Lobby Novotel Airport Banjarmasin

05.00-08.00 Lok Baintan Floating Market

08.00-10.00 Touring to the Martapura RIver

10.00-11.30

11.30-13.00

13.00-15.30

15.30-16.00

Visiting Curiak & Bakut Island

Lunch break at Soto Bang Amat

Shopping at CBS Market

Back to Lobby Novotel Airport Banjarmasin


8

PARALLEL SESSION (Conservation and Management & Sustainable Tourism)

FRIDAY 1st NOV 2019

No. Time Tittle Presenter Room

1. 16.00 – 16.10 In-Situ and Ex-Situ Conservation of Medicinal Plant Pikajar (Schizaea digitata) in South Kalimantan, Indonesia

Yudi Firmanur Arifin

Room A

2. 16.10 – 16.20 Engaging Palm Oil and Hot Spot Area to Mitigate Forest Fires

Ariesta Lestari

3. 16.20 – 16.30 Study of Potential Pollution of Sasirangan Liquid Waste with Biological and Chemical Parameters

Isna Syauqiah

4. 16.30 – 16.40 Cereal Flakes as Emergency Food from Nagara Sweet Potato (Ipomoea batatas L.) and Ikur-Ikur (Setaria italica) and Its Characteristics During Storage

Ratna Ariani Nilateja Putri

5. 16.40 – 16.50 Formulation of Instant Porridge Product Based Flour Ikur-Ikur (Setaria italica) As An Alternative Emergency Food

Sindy Yulesi

6. 16.50 – 17.00 Benefits of Biochar Compost (BIOCHOM) To Yield Rice Productivity On Tropical Swampland, South Kalimantan, Indonesia

Wahida Annisa, Evi Setyawaty

7. 17.00 – 17.10 Efficiency and Water Management for Green Beans in Acid Soil, authornya Wahida Annisa and Hendri Sosiawan

Wahida Annisa, Hendri Sosiawan

8. 17.10 – 17.20 The Potential of Mangrove Forest as Natural Tourism Area Based on the Flora-Fauna Characteristics and Social Aspect Case Study: Mangrove forest in Angsana Village

Kissinger

9. 17.20 – 17.30 Rehabilitation of Peatland (Repeat): Volunteer Tourism and Restoration in Tumbang Nusa Research Forest

Safinah Surya Hakim

10. 17.30 – 17.40 Protein Content In Muscle, Liver And Gonad Of Mudskipper Fish (Periophthalmodon Schlosseri) In Kuala Lupak Village, South Kalimantan

Abdul Hakim, Hidayaturrahmah,

Rani Sasmita

11. 17.40 – 17.50 Identification of Nematodes in the Sweet Potato (Ipomea batatas ) Rizosphere at Tanah Laut District, South Kalimantan Province

Riyan Adi May Sandi, Abdul Gafuur,

Dewi Fitriyanti

• Chair: Sunardi, S.Si., M.Sc., Ph.D


9

PARALLEL SESSION (Wetland Ecosystem & Wetland Biodiversity)

FRIDAY 1st NOV 2019

No Time Tittle Presenter Room

1 16.00 – 16.10 Natural Regeneration 3 Years after Fire of Natural Peat Swamp Forest in Merang Area, Musi Banyuasin District, South Sumatra

Adi Kunarso, Tubagus Angga

Anugrah Syabana

Room B

2 16.10 – 16.20 Study Of Heavy Metal Pollution (Pb And Cu) And Bacterial Attendance In Mudskipper, Eeltail Catfish And Mud Clam From Kuala Tambangan Mangrove Ecosystem

Anang Kadarsah, Dafiuddin Salim,

Krisdianto, Ika Oksi Susilawati

3 16.20 – 16.30 Biomassa Of Bundung (Scirpus Grossus L) On Wetland At Jejangkit Muara Village South Borneo Barito Kuala Regency

Linda Putri Utari, Krisdianto, Anang

Kadarsah,

4 16.30 – 16.40 Evaluation Of Kratom (Mitragyna speciosa) Habitat In Bangkuang Village, District South Barito Central Kalimantan

Mia Rahmadaniati, Krisdianto,

Chatimatun Nisa

5 16.40 – 16.50 Analysis Of Mangrove Tree As Proboscis Monkey (Nasalis larvatus) Staple Food In Research Post At Curiak Island On Barito Kuala District

Napisah, Krisdianto, Chatimatun Nisa

6 16.50 – 17.00 Molecular Sexing in Lovebird Fischer (Agapornis fischeri) Using Polymerase Chain Reaction Method

A. D. Argarini, M. Purwaningrum, A.

Haryanto

7 17.00 – 17.10 Genetic Diversity of Javan Deer (Rusa timorensis) Based on ATP Synthase F0 Subunit 6 (ATP6) Gene

Aris Purwantoro

8 17.10 – 17.20 Comparison of Goblet Cell Density in Small Intestine and Large Intestine Sunda Porcupine (Hystrix javanica)

Ariana

9 17.20 – 17.30 Locomotion Study of Bekantan (Nasalis larvatus)

Avendi Rachma Jadi

10 17.30 – 17.40 Molecular Identification of Gobiidae Fish of Kuala Lupak Estuary

Badruzsaufari

11 17.40 – 17.50 Morphology Identification of the Fruit Bat’s Tounge (Rousettus amplexicaudatus) Using Scanning Electron Microscope (SEM)

Geraldus Gunawan

12 17.50 – 18.00 Morphological study by Scanning Electron Microscopy (SEM) of the lingual papillae in the Java Pipistrelle (Pipistrellus javanius)

Golda Saragih

13 18.00 – 18.10 The structure of seminal vesicle gland of sunda porcupine (Hystrix javanica)

Teguh Budipitojo, Syithia Regita Noor

Mahesty, Yosua Kristian Adi

14 18.10 – 18.20 Protein diversity and pylogenetic relationship of freshwater catfish (Siluroidei, Siluriformes) in South Kalimantan

Rani Sasmita, Mabrur, Ummy

Shaliha, Badruzsaufari

15 18.20 – 18.30 Detection of Cymbidium Mosaic Virus Of The Native Orchids Germplasm In South Kalimantan, Indonesia Using RT-PCR Method

Dindin H. Mursyidin

16 18.30 – 18.40 Morphological Characterization of Two Populations of Dorylaimid Nematodes from Curiak Island Bekantan Research Station, South Kalimantan

Abdul Gafur, Albert Oriya Gampung

• Chair: Dr. drh. Aris Haryanto, MP


10

PARALLEL SESSION (Wildlife Biology and Medicine)

FRIDAY 1st NOV 2019

No Time Tittle Presenter Room

1 16.00 – 16.10 Recombinant Fussion (F) Protein Newcastle Disease Virus Expression from Escherichia coli plasmid Clone C2a using AccuRapid™ Protein Expression Kit

Ahmad Pandu Satria Wiratama, Aris

Haryanto

Room C

2 16.10 – 16.20 The Expression of Serotonin In CA1 Hippocampal of Neurodegenerative Disease Rat Model Under Treatment Of Ethanolic Extract Ocimum Sanctum Linn.

Ulayatul Kustiati, et al.

3 16.20 – 16.30 The Effect of Secretome Administration on Glucose Response in Type 1 Diabetes Mellitus Rats

Dian Bekti Hadi Masithoh, Teguh

Budipitojo

4 16.30 – 16.40 The Expression of Neuropeptide Y (NPY) on The Hipocampus of The In Vivo Model Neurodegeneration after treatment of Ethanolic Extract Ocimum sanctum Linn.

Made Bagus Auriva M

5 16.40 – 16.50 Ocimum sanctum ethanolic extract inhibits cell viability and induces apoptosis on A549 lung cancer carcinoma

Puspa Hening

6

16.50 – 17.00

Structural Recovery of Liver Failure Induced by Secretome

Teguh Budipitojo, Dian Bekti Hadi Masithoh,

Neila Rahma Habibah

7 17.00 – 17.10 Physiological Values, Conjunctiva Color And Feces Form As A Health Parameter In Bekantan (Nasalis larvatus)

Soedarmo Indarjulianto

8 17.10 – 17.20 Discovery Of Malaria Cases In Forest Workers In Teluk Kepayang, Tanah Bumbu, South Kalimantan In 2018

Nita Rahayu

9 17.20 – 17.30 Blood Glucose Level of White Rats (Rattus norvegicus) After Giving Catfish Biscuit (Pangasius hypopthalmus)

Hidayaturrahmah

10 17.30 – 17.40 PROAPOPTOSIS EFFECT OF ROOT OF EURYCOMA LONGIFOLIA (PASAK BUMI) ON THE PROSTATE CANCER : IN SILICO ANALYSIS

Eka Yudha Rahman, Mulyohadi Ali, Basuki Bambang Purnomo,

Nia Kania

11 17.40 – 17.50 The Correlation of Femur Length with Body Length of Proboscis Monkey

Ariana, Irma Padeta, Arvendi Rachma Jadi, Hery Wijayanto, Teguh

Budipitojo, Slamet Raharjo, Amalia

Rezeki, and Heri Budi Santoso

12 17.50 – 18.00 Immunolocalization of 3β-hydroxysteroid dehydrogenase in Regeneration of Testicular Dysfunction by Mesenchymal Stem Cell-Conditioned Medium in Adult Male Wistar Rat

Linda Miftakhul Khasanah, Teguh

Budipitojo, Yuda Heru Fibrianto

13 18.00 – 18.10 Secretome Improve Androgen Binding Protein (ABP) Production in Testicular Dysfunction Rats Induced by Cisplatin

Surya Agus Prihatno

14 18.10 – 18.20 Immunolocalization of Basic Fibroblast Growth Factor on Sunda Porcupine’s Skin

Irma Padeta, Ariana Ariana, Teguh

Budipitojo

• Chair: Irma Padeta, M.Sc.


11

PARALLEL SESSION (Wetland Plant and Microbe Biodiversity)

FRIDAY 1st NOV 2019

• Chair: Hasrul Satria Nur, S.Si., M.Si.

No. Time Tittle Presenter Room

1. 16.00 – 16.10 The Abundance of Arbuscular Mycorrhiza Infective Propagules Under Galam Stand at Shallow Peat of South Kalimantan

Tri Wira Yuwati, Noveani Annisa Rusalinda Rahmi,

Badruzsaufari

Room D

2. 16.10 – 16.20 Exploration of Fungi from Endophytic and Rhizospheric of Galam Plant (Melaleuca cajuputi Powell.) That Has A Potency to Produce of Indole Acetic Acid (IAA)

Witiyasti Imaningsih, Safinah Surya Hakim, Nadiya Dwi Rahayu

3. 16.20 – 16.30 Analysis of Water Mimosa Leaves Proximate Content (Neptunia oleracea and Neptunia plena) and Its Potency in Improving Spermatogenesis Activity and Spermatozoa Quality of Male Rats

Rusmiati, Sasi Gendro Sari

4. 16.30 – 16.40 Chalcone Derivatives as Antibacterial Agents-A Review

Kamilia Mustikasari, Uripto Trisno Santoso

5. 16.40 – 16.50 Cytotoxic test of n-hexane extract of mundar (Garcinia forbesii King) fruit skin

Kholifatu Rosyidah, Muhammad Alfian Rizki,

Maria Dewi Astuti, Rodiansono

6. 16.50 – 17.00 Identification of Chemical Compounds from Nypa fruticans Wurmb. Endosperm

Maria Dewi Astuti, Khairatun Nisa, Kamilia

Mustikasar

7. 17.00 – 17.10 Potential of Leaves of Water Mimosa (Neptunia Plena) Increasing Spermatogenesis Activity Male Rat

Rusmiati, Sasi Gendro Sari, Chindy Rizka

Febryandini Budyarti

8. 17.10 – 17.20 Cellulolytic bacteria isolated from swamplands activities and their effect on rice growth in acid sulfate soils

Yuli Lestari, Eni Maftu’ah, Wahida Annisa

9. 17.20 – 17.30 In Vitro Testing of Nitrogen-Fixing and Synthesizes IAA Hormones of Fungi Originated Peatlands Galam (Melaleuca cajuputi Powell) .

Siti Ko’idah, Witiyasti Imaningsih, Safinah

Surya Hakim

10. 17.30 – 17.40 Anatomical Stucture And Terpenoid Content Zodia (Evodia suaveolens Scheff) Leaves

Evi Mintowati Kuntorini, Dewi Nofaliana, Eny Dwi

Pujawati

11. 17.40 – 17.50 Morphological Characters and Nutrient Profiles of Water Mimosa (Neptunia oleracea and Neptunia plena) from Banjar Wetland

Sasi Gendro Sari1, Rusmiati2


12

POSTER PRESENTER

FRIDAY, NOVEMBER 1st 2019

No Name Presenter Title

1 Anna Hairani, Muhammad Alwi and Khairil Anwar

Poster Increasing Rice Yield through Amelioration and Fertilization On Medium Freshwater Swampland

2 Deby Kania Tri Putri, Fadel Amirda, Hilman Muzadi, Amy Nindia Carabelly, Isyana Erlita,

and Rahmiati

Poster The Antibacterial Activity Of Actinomycetes Against The Growth Of Streptococcus mutans and Lactobacillus acidophilus

3 Eti Heni Krestini, Ani Susilawati, and Catur Hermanto

Poster Effect of NPK Fertilizer and Microbial Consortium to Growth and Production of Garlic (Allium sativum L.)

4 Eti Heni Krestini, Ulya Rusmawati, and Ani Susilawati

Poster Effectiveness of Microbial Consortium on Growth, Yield and Intensity of Withered Disease (Fusarium oxysporum Schelecht) on Garlic Plants

5 Mawardi, Bambang Hendro Sunarminto, Benito Heru Purwanto, Putu Sudira, and Totok Gunawan

Poster The Influence of Tidal on Fe Distribution at Tidal Swamp Rice Farming in Barito River Area, South Kalimantan Indonesia

6 Niken Rani Wandasari and Soemarmo

Poster The Potential Used of Swampland for Rice (Oryza sativa L.) Production in Border Area for Agricultural Development

7 Vika Mayasari and Wahida Annisa

Poster Water Management for Agriculture in Peatland

8 Ulayatul Kustiyati Poster Oriental Pharmacy and Experimental Medicine Ocimum sanctum Linn. ethanolic extract inhibit the angiogenesis on the Human Lung Adenocarcinoma (A549) cells

9 Safinah Surya Hakim, Tri Wira Yuwati

Poster The Use of Fungal Endophyte Penicillum Citrinum in Tree Seedling: Applicability and Limitation

10 Arthanur Rifqi Hidayat Poster Impact of Land Reclamation On Acid Sulphate Soil And Its Reclamation

11 Sunardi Poster Eleocharis dulcis: A Practical Overview of Wetland- Based Materials

12 Bakti Nur Ismuhajaroh, Didik Indradewa, Budiastuti Kurniasih, Sri Nuryani Hidayah Utami, Rosalina Kumalawati

Poster Evaluation of Growth Environment of Water Lilies (Nymphaea pubescens Willd.) through Distribution Mapping in Non-tidal Swamp, Hulu Sungai Utara Regency, South Kalimantan

13 Vika Mayasari and Anna Hairani Poster Preliminary Prediction of Rice Planting for Planting Season 2019, 2020, and 2021 Based Water Balance in Freshwater Swampland

14 Wiwin Tyas Istikowati, Sunardi, Mochamad Arief Soendjoto

Poster Empowerment of Batu Tanam Community toward Apiculture Village

15 Rini Widayanti Poster Genetic variation of Proboscis monkey (Nasalis larvatus) from Bakut island- South Kalimantan based on mitochondrial Cytochrome B gene


13

INVITED SPEAKER


14

Importance of Tropical Wetlands in the Conservation of

Orangutans (Pongo pygmaeus)

Karmele Llano Sanchez1,*, Gail Campbell-Smith1

1Veterinary Medicine Spain, International Animal Rescue Indonesia, Ketapang, West Kalimantan, Indonesia

Abstract. Tropical Peat swamp forests are a unique ecosystem that is most extensive in Southeast Asia. They

are rich in both carbon and biological diversity, provide clean water, regulate climate, as well as provide other

life-supporting ecosystem services to the local communities. However, these tropical wetlands are also fragile

and under mammoth threat from logging, fires and land conversion, resulting in the loss of forest biodiversity.

Bornean orangutan (Pongo pygmaeus) populations are known to have drastically declined in the last 50-60

years due to habitat destruction (forest conversion and wild fires), as well as hunting, killing and the illegal

wildlife trade. Known to support orangutan populations at over twice the densities that other habitat types,

makes the survival of these tropical wetlands of quintessential importance for the conservation of wild

orangutans. Changing the integrity of these wetlands via forms of anthropogenic disturbances (drainage,

burning, etc.), not only severely threatens dwindling wild orangutan populations and other flora and fauna

species, but contributes negatively to increased Green House Gas emissions that aggravate climate change,

and the lives of people who live or depend on these ecosystems. Protecting peatlands in Borneo has become

one of the most critically needed measures to save orangutans and protect the planet from irreversible effects

of climate change. This can be achieved by engaging and supporting local communities through the promotion

of eco-tourism, the development of non-timber products alternative incomes, or the development of payments

for ecosystem services.

* Corresponding author: [email protected]

mailto:[email protected]


15

Conservation of wetland dependent fauna – lessons learned

from case studies of the quokka Setonix brachyurus, European

bison Bison bonasus, European beaver Castor castor, and

Australian arid zone fauna.

Matt W. Hayward1

1School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia

Abstract. Using case studies of the quokka, bison, beaver and a suite of Australian arid zone fauna, I will

draw out some conservation lessons that are relevant to wetland dependent fauna. The quokka is a wallaby

that inhabits the headwaters of rivers and was one of the most abundant species of mammal when Europeans

arrived in the region, but declined precipitously in the 1930s with the arrival of the European red fox. Today,

fox control is fundamental to their persistence. Furthermore, quokkas have evolved to cope with Aboriginal

traditional burning regimes, and so modern prescribed burning practices need to be altered to improve the

probability of persistence for the species. Kalamurina Wildlife Sanctuary lies in the arid centre of Australia –

linking several protected areas. The river running through Kalamurina only flows every 3-5 years, but the

removal of cattle from the region has enabled the fauna to benefit from this flooding for the first time in 100

year. The reintroduction of European beaver to Wales has been supported by environment ministers for 18

years, but no action has been taken due to the government agency in charge being risk averse. The European

bison is considered a refugee species (driven to forest dependence by human hunting pressure), however its

ecology suggests it should be living along the riparian zones of rivers. Key lessons I have learnt from studying

these species are that 1) identifying the key threatening process driving the decline of a species is possible

and necessary to ensure its persistence; 2) human-derived artificial selection can mask the natural behaviour

of wildlife; and 3) governments are far more risk-averse than the private sector.




16

Urban Wetland Ecosystems: Sustaining Biodiversity and

Enhancing Water Quality

Reuben Sharma1

1Centre for Wildlife Research and Conservation, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang,

Selangor, Malaysia

Abstract. Freshwater wetlands are known to be among the world’s most productive habitats that support a

wide array of fauna and flora. In addition to supporting biodiversity, the wetland environment is indispensable

for providing “ecosystem services” to humanity, including constant freshwater supply, food and building

materials, flood alleviation, groundwater recharge, maintaining surface water flow during dry periods, and

mitigating the adverse effects of global warming and climate change. The diverse microbes, plants and

wildlife that inhabit the wetlands are important components of the food chain and constitute vital modules in

the global cycles of water, nitrogen and sulphur, which maintain and support healthy atmospheric conditions.

It is therefore imperative that proper management of these wetland habitats and its associated biodiversity is

embraced as a global priority and immediate actions must be instituted to conserve this natural resource.

Urban wetlands may be natural or constructed and have traditionally served to mitigate storm water and to

manage urban effluents. The present talk will focus on how these urban wetlands can be reconstructed and

enhanced to provide additional ecological functions including promoting and sustaining faunal and floral

biodiversity, as well as enhancing water quality through phytoremediation.




17

The Revolution of New Genetic Tools for The Study of The

Ecology of Rare or Elusive Species, Using Non-Invasive

Approaches

Johan Michaux1,2

1Gecolab Laboratory, Institute of botany (Bat. 22). University of Liège, 4000 Liège Belgium 2 UMR CIRAD-INRA ASTRE (Animal, Santé, Territoires, Risques et Ecosystèmes), Department BIOS, Campus international de

Baillarguet, 34398 Montpellier Cedex 5, France

Abstract. Since the last decade, the development of new molecular biology technologies and particularly the

Next Generation Sequencing methods have revolutionized the study of biodiversity. Using non-invasive

approaches (collect of faeces, hairs, urine, saliva..), these methods presently allow to detect the existence of

cryptic species, to estimate gene flow among isolated populations, their population sizes but also to study the

ecology of rare or elusive species, like their diets, their microbiomes, their sex ratio, their daily movements

or their putative niche overlapping or hybridations with closely related species. More particularly, these new

genetic methods are interesting for the study of threatened species, in order to propose the best management

measures for them. Using different examples developed in my laboratory, I will enhance the interest of such

studies, as complementary tools to other methodologies developed in the fields. As an example, these studies

evidenced the most precise information concerning the diets and the mobility of the Polar bear populations

living in northern Canada, the European otter living in France, the Pyrenean Desman, the African lion and

different Asian mammal species. These informations are of a prime interest to better understand the impact

of habitat destruction on the food availability, and how these threatened species can adapt themselves to

survive to global changes. On a more fundamental aspect, these studies evidenced how species having a close

ecological niche, like the Pyrenean desman and the aquatic shrew can live in a same area, by shifting their

diets, in order to avoid competition.




18

The Role of Sahabat Bekantan Indonesia in Conservation of Bekantan in Indonesia

Amalia Rezeki1

1Sahabat Bekantan Indonesia, Jalan Sultan Adam, Banjarmasin, 70123

Abstract. Bekantan is an endemic primate of Kalimantan whose conservation status is currently threatened

with extinction based on the establishment of the world conservation organization International Union for

Conservation of Nature and Natural Resources (IUCN) since 2008. Based on this, the Government arrange

the efforts to develop a strategy and action plan for conservation of bekantan for 2013-2022. In order to support

and assist government programs, Sahabat Bekantan Indonesia (SBI) sounding the mission of "Save Bekantan".

The purpose of this paper is to describe the role of SBI in an effort to save bekantan based in South Kalimantan.

The method used is descriptive research by presenting primary data and secondary data. The results showed

that SBI's efforts in supporting conservation of bekantan were integrated in 5 work programs those are

education-awareness programs, evacuation-rehabilitation-release programs, habitat-restoration mangrove

restoration programs, research and community dedication programs, and sustainable tourism development

programs through bekantan ecotourism. These programs already have achievements that directly and

indirectly contribute to the conservation of bekantan. Although in its implementation, there were still four

major problems and challenges experienced by SBI. The commitment and consistency that is built in the

communication of volunteers at SBI institution becomes an optimistic mindset to achieve the goal of fighting

for bekantan and its endangered habitat.

*Corresponding author: sahabat [email protected]


19

How the local people face the rapid change of the tropical

wetland environment?

Krisdianto1*, Slamat2, Pahmi Anshary2, Anang Kadarsah1, Ika Oksi Susilawati1

1Lambung Mangkurat University, The Faculty of Mathematics and Natural Science. 2Lambung Mangkurat University, The Faculty of Fisheries and Marine Science.

Abstract. Barito River crosses from the northeast of Central Kalimantan province, but almost two-third of

1000 km length lies in South Kalimantan. Despite water come from Schwaner mountain in the upper river,

this river receives water from Meratus pass through 5 big rivers, such as Tabalong, Batang Alai, Pagat,

Amandit, Riam Kiwa. Before pouring into Barito, the water retains for sometimes in the low land swampy

area, shaping huge lowland muddy to shallow lake, simply named wetland. How do people commit to

sustainability living in the extreme tropical wetland for generations? Ecological investigation was conducted

for 2016 – 2019, in-situ observation on the daily life-behavior of local people of 200 local people living Barito

Kuala, Tapin, Hulu Sungai Selatan, Hulu Sungai Tengah, Hulu Sungai Utara residences, their technologies,

their progress in sustainability development. Data was collected by interviewing directly to the local, face to

face, meeting with family, observing and confirming nature with them, studying scientific articles and grey

literature, from officially reports. Statistical calculation helps to compare several data sets data, as well as

serial of photographs, were documented to support visually the evidence. In conclusion, the locals face the

rapid change environments which influence their life, income, health and their properties and prosperity,

Mostly they complain that life is much more difficult now than several decades ago.

*Corresponding author: [email protected]



20

The Big Nose of Bekantan (Nasalis larvatus)

Hery Wijayanto1*

1 Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada

Abstract. Bekantan (Nasalis larvatus) is very unique non-human primate, characterized by a big nose,

particularly the adult male. Besides that, this very interesting species also toes webbed and put bekantan as

the best non-human primate swimmer. The researchers are very curious with the big nose of Bekantan. Most

of them hypothesized the big nose with the adult female bekantan behavior that chooses to mate with the adult

male bekantan with bigger nose. The next question is “why the female bekantan choose big nose adult male”?

The fact that they are toes webbed non-human primate is clearly related with their ability to swim. Moreover,

bekantan also supported by special valve prevent water entering nasal cavity during swimming. Bekantan big

nose is not only caused by natural selection, perhaps they need such anatomic form for their aquatic life style.

As good swimmer, bekantan can swim and dive under water to run from the terrestrial predator or just to cross

the river. However, the information linked between big nose shape and the evolution history except natural

selection (female tend to mate with bigger nose male) is remain deficient.




21

Response of Peat Soil’s Microbes to Drainage and Forest Fire

Abdul Hadi

Department of Soil Science, Lambung Mangkurat University, Jl. A. Yani KM 36 Banjarbaru, Indonesia

Abstract. Peat soil is technically a soil which contains appreciable quantities of organic matter that is

considered to dominate the soil properties. It has been estimated that the total area under peat land is around

240 million ha world over, including about 14.9 million ha occurred in Indonesia and 300,000 ha occurred in

Japan. Peat soils in Indonesia and Japan have for long time been used for paddy cultivation. Some portions

of peat soils in Indonesia are also use for estate crop cultivation like coconut, pine apple, and presently oil

palm. The utilization of peat soil for estate crops cultivation is commonly started by constructing drainage

ditched to release the excessive water. Over drain, coupled with human carelessness, may lead the peat soil

to front of forest fire. Intermittent drainage is practiced in paddy filed to remove hazardous substance from

paddy filed. Peat soil has attracted considerable attentions globally due to its potent as source of greenhouse

gases (GHGs) and subsequent global warming. This paper is aimed at summarizing the response of microbes

to drainage and forest fire of peat soils. The information was mainly gained from the studies carried out by

author in Indonesia and Japan, using plate count, adenosine tri phosphate (ATP) measurement, and

denaturized gradient gel electrophoresis (DGGE) methods. The results indicate that forest fire and intermittent

drainage (draining peat for less than 6 days) did not affect the population and community structure of GHG

related-microbes. However, the intermittent drainage and forest fire reduced the activities of methanogens

and methanotrophs, respectively. Moreover, long term drainage affects the population of nitrifiers and

denitrifiers. Water filled pore space (WFPS) seemed to be the most influential soil properties to GHGs related-

microbes in responding drainage and forest fire.



22

ABSTRACTS

ORAL PRESENTATION


23

CONSERVATION AND MANAGEMENT


24

In-Situ and Ex-Situ Conservation of Medicinal Plant Pikajar (Schizaea digitata) in South Kalimantan, Indonesia Yudi Firmanul Arifin1,2, Siti Hamidah1,2

1Faculty of Forestry, Lambung Mangkurat University 2Center of Excellence for Innovation, Technology, Commercialization, Management: Forest and Wetlands

Abstract. South Kalimantan Province has a high wealth of biodiversity, and most of it has potential as a

medicinal plant that can be useful for human health. One of the plants known to play a role as a drug is pikajar

(Schizaea digitata). This species has long been known to ethnic Dayak Meratus in South Kalimantan as

traditional medicine. It is believed to treat various diseases, including those believed to be useful for

overcoming impotence, maintaining stamina and lumbago. Conservation efforts are not done yet, so this

species will be endangered. This study was conducted to determine the characteristic habitat, growth of

pikajar in-situ and ex-situ cultivation, and ex-situ cultivation after treatments as well. S. digitata tended grow

well on habitats which have at altitude 164-214 m asl. It can grow well on soil with pH of 4.3-4.6, N of 0.12-

0.15, P of 0.15-0.90mg/100g, C of 2.0-2.2%. This species was living generally in secondary forest which had

a micro climate is very variable, temperature of 28-37°C, humidity of 56-89%, and low light intensity of 2.1-

14.3%. Leaves growth in-situ cultivation is 50% higher than the growth in the number of leaves in situ.

Likewise leaves drying are less for in-situ cultivation than ex-situ. This means that the growth of pikajar in

the in-situ is still better than the ex-situ, but from the ability of adaptation and life of both the in-situ and ex-

situ successfully.




25

Engaging Palm Oil and Hot Spot Area to Mitigate Forest Fires

Ariesta Lestari1 and Katriani Puspita Ayu2*

1Ph.D candidate, Monash University, Australia 2Ph.D candidate, Leiden University, Netherlands

Abstract. Forest fire is one of environmental problem happens in Central Kalimantan. The fire does not only

damage the forest ecosystem and biodiversity, but also threat the health and socio-economic of local people.

Forest fire in Central Kalimantan is widely known as human-made, such as the process of shifting cultivation

and land clearing. The expansion of forest into palm oil plantation is often blamed as the cause of forest fire,

since the forest clearing involves a huge amount of fires. Therefore, the aim of this study is to explore whether

the existence of palm oil cultivation contributes to the occurrence of forest fires. We used satellite imagery of

hotspot, and overlay it with the land use data to generate the fire risk zone map using geographic information

system (GIS) method. Through the map, the risk of fire can be monitored in advanced in order to help the fire

authority provide the act of mitigation. The result of this study suggested that risk mapping is vital for the

forest fire management to mitigate the spread of forest fire. The region to be fire-prone within the palm oil

cultivation is suggested to form a preventive act through an active forest-fires monitoring. In sum, this study

is expected to provide a map of forest fires’ risk around the cultivation area especially palm oil plantation,

and help the fire authorities as well as stakeholders to identify the risk zone for fires prevention in the future.




26

Study of Potential Pollution of Sasirangan Liquid Waste with

Biological and Chemical Parameters

Isna Syauqiah1,2, Desi Nurandini1*, and Riani Ayu Lestari1

1Department of Chemical Engineering, Faculty of Engineering, Lambung Mangkurat University, South Kalimantan, Indonesia

2Wetland Based Materials Research Group, Lambung Mangkurat University, South Kalimantan, Indonesia

Abstract. Sasirangan - the traditional fabric, claimed as an icon of Banjarmasin and core part of South

Kalimantan identity - has been produced for a long time by home industries and contributes to local

community economic development. Practically, activities of Sasirangan home industries use several chemical

coloring agents that produce liquid waste and have anegative impact on the ecological life of the Martapura

River in Banjarmasin. The purpose of this study is to identify the potential of liquid waste of sasirangan for

pollution in the Martapura River. Certainly, It would importantly contributes in order to provide solution to

overcome these waste effects on the surrounding environment. This study used the observation method and

survey approach to Sasirangan home industry in three locations around the Martapura River in Banjarmasin

City. Sampling was conducted both at the point of inlet and outlet of these locations. Then, the samples were

laboratory tested and quantitatively analyzed for biological parameters (total Cr and total Cd content in

Snakehead Fish) and chemical parameters (BOD, COD, pH, and oil and fat content). The results showed that

wastewater content of sasirangan generally were still within the limitation of quality standards permitted

according to the Governor of South Kalimantan Regulation Number 04 Year 2007. Nevertheless, there were

still several parameters that exceed the quality standard, for content of heavy metal chromium in Snakehead

Fish with value of 0.456 mg/kg and COD parameters of the upstream sample inlet (Tati Sasirangan) with

value of 348.9 mg/L.




27

Cereal Flakes as Emergency Food from Nagara Sweet Potato

(Ipomoea batatas L.) and Ikur-Ikur (Setaria italica) and Its

Characteristics During Storage

Ratna Ariani Nilateja Putri1, Alia Rahmi1, Agung Nugroho1

1 Department of Agro-industrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru, South Kalimantan

70714

Abstract. Emergency food is a type of food that can supply the daily energy needs of humans during

emergency situations such as natural disaster. It also offers a practical serving and a long shelf life. Cereal

flakes comply with the criteria of emergency food. This research aimed to develop cereal flakes from nagara

sweet potato and ikur-ikur which are ingredients from local resources in South Kalimantan. This study used

a Completely Randomized Design (CRD) with 3 treatments and 2 replications. The treatment is the

composition of nagara sweet potatoes and ikur-ikur (75:25), (50; 50), and (25:75). Cereals flakes on day 0

were tested for proximate levels (moisture content, ash content, protein content, fat content, carbohydrate

content), crude fiber content, TPC, and sensory (hedonic and scoring). Based on observations on day 0, cereal

flakes (25:75) showed the best nutrition value with 0,55% moisture content, 2,24% ash content, 11,40%

protein content, fat content 20,70%, carbohydrate content 65,13%, crude fiber content 6,76%, calories 492,29

cal/100g, and TPC 8,5x101 colonies/ml. Cereal flakes (75:25) obtained the best hedonic taste and aroma rating

on day 0, therefore, this treatment was used for shelf life observation for 40 days at 40⁰C and 70% RH. Forty

days observation showed that cereal flakes (75:25) remained in good quality for consumption based on SNI

01-42017-1996. Whereas the production costs for cereal flakes of nagara sweet potato and ikur-ikur with

formulation (75:25) are Rp.13.295,00/100g, formulation (50:50) of Rp.14.337,25/100g, and the formulation

(25:75) of Rp.15.379,50/100g.




28

Formulation of Instant Porridge Product Based Flour Ikur-Ikur

(Setaria italica) as an Alternative Emergency Food

Sindy Yulesi1, Alia Rahmi1, Dessy Maulidya Maharani1, Agung Nugroho1

1Department of Agro-industrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru, South Kalimantan

70714

Abstract. Ikur-ikur plant (Setaria italica) are one of the local commodities that can be processed into

emergency food such as instant porridge because it contains high calories. This research aims to obtain an

instant porridge formula from ikur-ikur that meet the requirements as emergency food and have the best

sensory reception. Seed from ikur-ikur plant are milled and sifted into flour, so that will obtained two kinds

of flour that is ikur-ikur flour without epidermis (B1) and ikur-ikur flour with epidermis (B2). Four

formulations for each flour that is (flour: water = 1:2, 1:3, 1:4. 1:5). Based on analysis of variance (ANOVA)

comparison factor of B1 and B2 flour with water did not significant effect on water content, ash content,

protein content, fat content, crude fiber content, carbohydrate content, total calories, and specific volume but

single factors such as flour type significant effect on water content, ash content, crude fiber content, and

specific volume value. P3 formula (B1; ratio 1:4) shows the best chemical characteristics, that is: water

content (6,60%), ash content (0,24%), protein content (10,10%), fat content (1,66%), crude fiber content

(1,75%), carbohydrate content (81,42%), total calories per 100 grams (381,01 kcal), and specific volume (4,35

cm3/g).




29

Indigenous Knowledge Cultivation of Tidal Swamp Rice Siam

Saba And Siam Mutiara

Izhar Khairullah1

1Indonesian Research Institute for Swampland Agriculture

Abstract. Tidal swampland at present and in the future is very strategic as one of national food barns

considering slopingof productive land. In South Kalimantan, for example, tidalswamp paddy fields are

available which is quite large, but the most are still planted with local varieties. More than 70% of rice

cultivation in the tidal swampland is planted with various local varieties. Existence of local rice varieties is

inseparable from its adaptability and acceptability factors. Among local varieties that are popular and widely

planted by farmers are Siam Saba and Siam Mutiara varieties. Siam Saba and Siam Mutiara were released as

local-improved varities. Both of these varieties have their respective advantages, both from distribution of

plant adaptation and potential benefits of yield. Siam Saba variety is widespread in tidal swampland of acid

sulphate soil types and soils that are seawater intrusion in dry season (near the coast) with A / B overflow

type, while Siam Mutiara in acid sulphate tidal paddy fields with B / C overflow type. Some of advantages of

Siam Saba compared to other local varieties are high yield potential of 4.70 t / ha, large number of tillers,

small and slender grain shape, white rice color, and amilose content of 81.69%. Whereas superiority of Siam

Mutiara is high yield potential of 4.80 t / ha, color of clean yellow grain, color of clear and trunsluscent rice

such as pearl, and amilose content of 48.88%. Both varieties showed uniform in growth, flowering time, and

ripening simultaneously, in mature panicles with almost no green grains, and high percentage of unfertile

grains. Local rice cultivation technology (Siam Saba and Siam Mutiara) indigenous knowledge here includes

seedbed, transplanting, land preparation, fertilizing, pest disease control, harvest and post-harvest processing

or yields carried out by indigenous farmers. This indigenous cultivation technology until now in tidal swamp

paddy fields of South Kalimantan is still widely practiced by farmers, especially for ownership of narrow or

limited rice fields. There were advantages and disadvantages of each in the practice of indigenous cultivation

technology.




30

Benefits of Biochar Compost (BIOCHOM) to Yield Rice

Productivity on Tropical Swampland, South Kalimantan,

Indonesia

Wahida Annisa1 and Evi Setyawaty2

1Indonesian Swampland Agricultural Research Institute (ISARI), Banjarbaru 2Institute of Research and Standardization of Industry Banjarbaru, Jl Panglima Batur Barat, Banjarbaru 70711

Abstract. Biochar improves chemical and fisic of soil quality. Biochar is a soil amendment created through the

burning of biomass, has a potential solution forwide-ranging environmental management problems. Biochar has

been shown to have positive outcomes on soil function to sequester carbon to slow the effects of global climate

change. The objective of this research was to study of the effect of compost biochar in increasing rice yields in

tropical swamp land. The design used in this study was a Randomized Block Design namely: (1) Biochom 1

(Without compost Harvest Waste + Without Biochar Rice Husk), (2) Biochom 2 (Compost Harvest Waste 5 t /

ha), (3) Biochom 3 (Biochar 3 Rice Husk 5 t / ha), (4) Biochom 4 (Combination of Harvest Waste Compost 2.5 t

/ ha + Biochar Rice Husk 2.5 t / ha). The results showed that application of biochom 4 Combination of Harvest

Waste Compost 2.5 t / ha + Biochar Rice Husk 2.5 t / ha) increased of rice yield reached 15.21% compared to

biochom 2 treatment (Compost Harvest Waste 5 t / ha) and reached 17, 78% with Biochom 3 treatment (Biochar

3 Rice Husk 5 t / ha), compost without biochar treatment.




31

Efficiency and Water Management for Green Beans in Acid Soil

Wahida Annisa and Hendri Sosiawan

Indonesian Swampland Agriculture Research Institute (ISARI)

Abstract. Water requirement for maximum production of green bean at 60 to 120 day after seeding varies between 300

and 500 mm depending on climatic conditions. Irrigation of green bean is very important because of the short vegetation

period and its rapid growing. Frequency of irrigation varies between 3 and 12 days depending on climate, crop stade

development and soil type. Drought and water shortages have occurred in almost all parts of Indonesia, especially in the

dry areas and for agricultural development need to be supported by the appropriate irrigation technology alternatives. The

experiment was conducted in a greenhouse, in a completely randomized design with two factors. The first factor was type

of irrigation, i.e., 40, 60, 80 and 100 % crop water requirement, while the second factor was the varieties of green bean, i.e.,

Merak and Vima 1, with three replicates respectivelly. the results showed that, significant effect of the interaction and

decreasing linear fits in response to salinity occurred for all variables studied. It was possible to note that drip irrigation

reduced the negative effects of irrigation water salinity on green bean yield and increased water use efficiency. According

to the results, water requirement for green bean plants during growth is 320.72 mm. The Vegetative performance of green

beans was shown by plant height and positively correlated with yields. The response of giving the amount of irrigation water

to plant height parameters shown at 30 DAP. The varieties of Vima 1 was signficantly responsive and adaptive to water

stress




32

SUSTAINABLE TOURISM


33

The Potential of Mangrove Forest as Natural Tourism Area Based on the Flora-Fauna Characteristics and Social Aspect Case Study: Mangrove forest in Angsana Village

Kissinger1,2, Noor Alfi Syahrin2, Rina Muhayah NP.1, Violet1

1Faculty of Forestry University of Lambung Mangkurat Banjarbaru South Kalimantan Indonesia 2Magister Program of Natural Resources and Environtmental Management University of Lambung Mangkurat Banjarbaru

South Kalimantan Indonesia

Abstract. Natural tourism is tourism activity which able to improve the welfare of the community by

reorganizing a variety of potential natural resources in a sustainable manner that is supported ecologically,

economically and socially to the environment and communities. Mangrove forests as natural resources have

potential value as a natural tourism. This research aims to analyze the potential of mangroves based on the

ecological perspective of flora fauna and socio-cultural to be a natural tourism area. The object of this research

is the mangrove forest and its resources. The location of research was Angsana Village, Angsana District,

Tanah Bumbu Regency, South Kalimantan Province, Indonesia. The equipment used is a set of survey tools,

software, and questionnaires. The characteristics of flora fauna in mangrove forest are determined by the

composition of flora and fauna. The species compositions were analyzed by tabulation matrix. Socio-cultural

aspect were analyzed descriptively. The potential of mangrove forests as natural tourism was analyzed using

a tabulation matrix and narrative descriptive. The composition of species trees found as a whole are 14 species.

There are 19 species of birds, 6 species of mamals and 7 species of fish founded in mangrove forest area. The

community strongly agree that mangroves can be used as a tourist area. Angsana Village has mangrove

vegetation around the river with an area of ± 43.61 Ha, the condition is still natural, access road to the

mangrove area is available. Mangrove forest in the village of Angsana which has a distance of ± 200 meters

from Angsana Beach. Based on an analysis of the potential of mangrove forests, this area has potential to be

developed as a natural tourism and even ecotourism. Mangrove natural tourism area can be developed as

additional tourism objects that are already running from Angsana Beach tourist destinations.




34

Rehabilitation of Peatland (Repeat): Volunteer Tourism and

Restoration in Tumbang Nusa Research Forest

Safinah Surya Hakim1 and Purwanto B Santosa1

1Research and Environmental Development and Forestry Center Banjarbaru, Jl. Ahmad Yani Km. 28,7 Landasan Ulin Banjarbaru,

South Kalimantan 70721 phone (0511)4707872

Abstract. Repeat (Rehabilitation of Peatland) is a volunteer tourism which initiated to raise awareness of

the sustainability of tropical peatland. In early of 2016, Repeat was initiated, and until 2018, about 1600

people participated, 21 hectares of post fire peatland were replanted and about thousands seedling were

planted. In this paper, we would like to study the characteristic of visitor who participated in Repeat activity.

A total of 75 questionnaire were randomly distributed to repeat participant. The result show that, most

participant already know the importance of tropical peat land, the cause that lead peat degradation, and its

impacts of peat degradation to environment and community. In some organization or school, particularly

which located near to Palangkaraya, Repeat became an alternative activity for school or group outing. Hot

weather, difficult access, and low information (interpretation) during activities are three main obstacles which

perceived by the Repeat Participant. However, about 75% of participants are willing to re-join Repeat activity

in the future. This means, Repeat proven to be able to be tourism activity that increase public awareness in

peatland and environmental sustainability.




35

Protein Content in Muscle, Liver and Gonad of Mudskipper Fish

(Periophthalmodon schlosseri) in Kuala Lupak Village, South

Kalimantan

Abdul Hakim1, Hidayaturrahmah1, Rani Sasmita1

1Department of Biology, Faculty of Mathematics & Natural Science, University of Lambung Mangkurat, South Kalimantan, Indonesia

Abstract. Protein is one of the compounds in the form of macromolecules which found in every organism

and a source of energy for the body of living things. The function of proteins is also for cell growth and development, tissue or organ. The purpose of this study was to determine the protein content in muscles, liver

and gonads of mudskipper fish (Periophthalmodon schlosseri) based on weight groups and the difference of

the sampling place in the waters of the village of Kuala Lupak, South Kalimantan and to determine the correlation protein content with weight groups and the difference of the sampling place. The protein was

analyzed by Kjeldahl method and Bradford method. Protein value obtained by using the Kjedahl method is

the protein content of the mudskipper fish muscle with value of 41.47% -71.57% with an average of 57.23%.

The protein content in the liver of mudskipper fish with value of 34.31%-45.8% with an average of 38.97%. The protein content in the gonad organs of mudskipper fish value of 30.32%-42.7% with an average of

36.39%. The highest value of the protein content of the mudskipper fish based on the place of taking in the

river is the muscle organ with an average of 49.27%. The ovarium organs of the mudskipper showed an

entirely optimal protein value of 4.054 ± 0.028 mg/ml. The analysis presented the obtained values of sample were not correlated either contrasted in protein content with the mudskipper organs based on differences in

the collected place. The high protein content of the mudskipper is affected by their habitat. It is also caused

by eating habits, maturity, season and feed availability.




36

Identification of Nematodes in the Sweet Potato (Ipomea batatas

) Rizosphere at Tanah Laut District, South Kalimantan Province

Riyan Adi May Sandi1, Abdul Gafur1, Dewi Fitriyanti2

1Department of Biology, Faculty of Mathematics & Natural Science, University of Lambung Mangkurat, South Kalimantan, Indonesia 2Plant Protection Study Program, Faculty of Agriculture, Lambung Mangkurat University

Abstract. Nematodes are one of the pests of plant diseases that can attack sweet potatoes. But it is not only

parasitic nematodes that play a role in the growth of plants and soil ecosystems, non-parasitic nematodes also

have their own role in the soil. Identification is the first step to understanding the role of nematodes, especially

on sweet potato plantation land in Tanah Laut Regency, South Kalimantan. This study aims to identify the

types of nematodes found in the rizosphere of sweet potato (Ipomea batatas) plantations in Tanah Laut

Regency. Sampling location are located in Atu-atu village and Pabahanan village, Tanah Laut Regency. Soil

samples taken at depths between 0-20 cm from the ground surface. Soil samples were filtered using the

Whitehead & Hemming (1965) method, to separate the nematodes from the soil. The nematodes are fixed

using formalin and preservative solutions for more than two weeks. Furthermore, Nematodes are processed

into permanent preparations on object glass using glycerin and paraffin, so that they can be identified under a

microscope. The results show that the nematodes were the genus Prionchulus, Iotonchus, Helicotylenchus, and

Criconemella.




37

WETLAND ECOSYSTEM


38

Natural Regeneration 3 Years after Fire of Natural Peat Swamp Forest in Merang Area, Musi Banyuasin District, South Sumatra

Adi Kunarso1 and Tubagus Angga Anugrah Syabana1

1Environment and Forestry Research and Development Institute of Palembang, Jl Kol H Burlian Km 6.5 Punti Kayu Palembang, South

Sumatra

Abstract. This study examines natural regeneration of tropical peat swamp forest after wildfire. The study

area is located in Merang Peat Swamp Forest (PSF) in Musi Banyuasin District. Merang PSF is situated over

a large peat dome that stores huge amount of carbon. 23 sample plots were established in the area burned in

2015. The regeneration of understorey, seedlings, and saplings was recorded in each plot. Meanwhile, peat

depth and its maturity were identified to determine the soil characteristic of study area. After 3 years of fire,

the area already covers densely by understorey mainly pakis udang (Stenochlaena palustris), pakis tanah

(Nephrolepis exaltata), and resam (Gleichenia linearis) with depth of root zone reach about 40 cm in some

plots. The native peat swamp species showed minimal regeneration during third years after fire due to

understorey covering and limited number of surviving trees. The natural regeneration of woody species was

dominated by bangun-bangun (Melicope glabra) and sepongol (Evodia sambuciana) which were calculated

about 371 saplings/ha and 200saplings/ha respectively. Those all two species also founded in the seedlings

stage. The peat depth varied from 0.32 m to 4.71 m with peat maturity is dominantly hemic in the subsurface.

This regeneration information provides useful indications for restoration options, which could be done by

assisted natural regeneration.


https://www.google.com/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&ved=2ahUKEwiplbjM-4DhAhXt6XMBHbpbCcMQjhx6BAgBEAM&url=http%3A%2F%2Ffloranegeriku.blogspot.com%2F2011%2F06%2Fpaku-udang-stenochlaena-palustris-bedd.html&psig=AOvVaw0kR0tH7Mru6Zlidkwk26q2&ust=1552629995401767


39

Study of Heavy Metal Pollution (Pb and Cu) and Bacterial

Attendance in Mudskipper, Eeltail Catfish and Mud Clam from

Kuala Tambangan Mangrove Ecosystem

Anang Kadarsah 1,*, Dafiuddin Salim 2, and Krisdianto1, and Ika Oksi Susilawati.1

1Biology Study Program, Faculty of Mathematics and Natural Sciences, Lambung Mangkurat Unviersity, A Yani Street Km 35,8

Banjarbaru, South Kalimantan 70714 2Marine Science Study Program, Faculty of Fisheries and Marine Science, Lambung Mangkurat Unviersity, A Yani Street Km

35,8 Banjarbaru, South Kalimantan 70714

Abstract. This study purpose is to detect the heavy metals presence of Lead (Pb), Copper (Cu), and

Mercury (Hg) in water bodies and sediments and also the presence of bacteria in three types of animals

(mudskipper, eeltail catfish, and mud clam) that inhabit mangrove ecosystems in Kuala Tambangan

Village, South Kalimantan Province. The research was conducted in June - September 2019.

Sampling method doing at the three points at the mangrove ecosystem estuary. The results showed

that the average of heavy metal content in waters and sediments both in the estuary, in the middle

and in the interior of mangrove ecosystem were above the standard based on the Decree of the

Minister of the Environment in 2004. In waters, Lead (Pb) levels is reached 0.11 - 0.128 mg/L and

Copper (Cu) at 0.028 - 0.444 mg/L, while in the sediment content is increased dramatically almost

10 times (Lead (Pb) 9,106 - 17,426 mg/gr and Copper (Cu) 14,284 - 20,576 mg/gr. In waters, The

highest content Lead (Pb) (0.128 mg / L) was found in the estuary while for Copper (Cu), the highest

(0.444 mg/L) on the interior. For sediment, the highest value of Lead (Pb) and Copper (Cu) was

found in the estuary with their respective levels are 17,426 mg/gr and 20,576 mg/gr. Then, The Total

Plate Count (TPC) values in three biota that inhabitants of mangrove ecosystems were below the

standard quality standard of SNI 2332.3-2015. Its about 950 colonies/gr on mudskipper, 100

colonies/gr on eeltail catfish and the highest on mud clam (24,300 colonies/gr). Qualitative test for

the presence of Eschericia coli, Salmonella, and Vibrio cholera shows a negative value

(<3AMP/g) in all test of animals. Colliform quantitative test shows that the value is above the

standard of quality (43 APM/gr in eeltail catfish) and the highest (150 APM/g) in mud clam. The

presence of parasites based on SNI 2332.6-2015 were not found on all samples. Heavy metal of Lead

(Pb) in eeltail catfish and mud clam is also can not be detected, but for Mercury (Hg) is successfully

detected above the standard of ISO 2354.6-2016, namely in eeltail catfish ± 0.1601 mg/kg and highest

in mud clam as much as ± 0.2653 mg/kg.




40

Biomassa Of Bundung (Scirpus Grossus L) On Wetland At

Jejangkit Muara Village South Borneo Barito Kuala Regency

Linda Putri Utari1, Krisdianto1, Anang Kadarsah1

1Department of Biology, Faculty of Mathematics & Natural Science, University of Lambung Mangkurat, South Kalimantan, Indonesia

Abstract. Bundung (Scirpus grossus L) is one of the most plant with tast growth in peatland area especially

in South Borneo Jejangkit Village Barito Kuala Regency. Many people doesnt know about the potential of

bundung that is why mostly from the community supposed that bundung only as a harmful pest for farmers.

The purpose of this research was observe the biomass of bundung with 2 sampling location (dry land and

wetlands) in Jejangkit Muara Village. Sampling method for soil and plant sample using plot size 1 x 1 m2 and

take it 30 clumps of bundung in each sampling location. The parameters in this research composed of : plant

height, leaf width, wet weight, dry weight, N, P, K content in soil samples at each sampling location. The

result on this research parameter water content 0,16%, dry weight 4,13 g, wet weight 73,62 g, plant height

84,6 cm, leaf width 2,55 cm in wet land area is higher than dry land area. Parameter N 0,14%, P 57,75 mg/100g

and K 0,66 me/100g in dry land area is higher that wet land area.




41

Evaluation of Kratom (Mitragyna speciosa) Habitat in Bangkuang Village, District South Barito Central Kalimantan

Mia Rahmadaniati1, Krisdianto1, Chatimatun Nisa2

1Department of Biology, Faculty of Mathematics & Natural Science, University of Lambung Mangkurat, South Kalimantan, Indonesia 2Faculty of Agriculture, University of Lambung Mangkurat, South Kalimantan, Indonesia

Abstract. Kratom (Mitragyna speciosa) originated Bangkuang village has varied habitat, these are across the

barito river and at mainlad of the village. Kratom howadays allegedly has a valuable price. The aim of this

research is to describe the habitation of Kratom based on growth of the tree height, stem circumference, leaf

area, water containing and the size of the canopy. The variable is measured by aech specipic measurement of

tree height, stem diameter, leaf area measurement, leaf weighing, canopy measurement and measurement of

water content. The results showed an average height of trees on the edge of the river 3.08 m and mainland of

2.57 m, an average number of diameters of tree trunks on the edge of the river 11.46 cm and on the mainland

9.65 cm. Leaf area and water content is greater at the edge of the river which is 124.2 mm for leaf 3, 147.8

mm for leaf 4, 176.4 mm for leaf 5, and leaf water content for 27.2%, leaf 4 29%, and leaf 5 35.9%. The

average canopy and canopy width on land is smaller with an average canopy of 13.22% and a canopy width

of 1.19 m. NPK analysis shows the N-total by the river is smaller than the P-total and K-total.




42

Analysis of Mangrove Tree as Proboscis Monkey (Nasalis

larvatus) Staple Food in Research Post at Curiak Island on Barito

Kuala District

Napisah1, Krisdianto1, Chatimatun Nisa1

1Department of Biology, Faculty of Mathematics & Natural Science, University of Lambung Mangkurat, South Kalimantan, Indonesia 2Faculty of Agriculture, University of Lambung Mangkurat, South Kalimantan, Indonesia

Abstract. Curiak island is one of mangrove tree area that becoming one of Proboscis monkey habitats outside

concervation area on South Borneo. It contains various kind of mangrove that become proboscis monkey

(Nasalis larvatus) staple food. The objective from this research is to find out various kind of mangrove trees

that become it’s staple food and bring about vegetation analysis of mangrove trees that is become staple food

of proboscis monkey in curiak island. Method that used to observe and measure of mangrove trees using

transect line as much as 30 plots of observation sized that 10 x 10 m. determination that used to mangrove

trees determined through observation proboscis monkey feeding activity from each kind of trees. The result

of this research pointed that there is four plant species that become staple food of proboscis monkey among

others : Rambai (Sonneratia caseolaris), Beringin (Ficus microcarpa), Waru laut (Hibiscus tiliaceus), and

Putat (Planchonia valida). The result from feeding activities shown that rambai plant (Sonneratia caseolaris)

more preferable from proboscis monkey with the average point of feeding duration in 40 minutes. Dencity

percentage of Rambai (Sonneratia caseolaris) is the highest (432 ind/ha) compared to another kind of trees,

thus with frequency (1 because it’s spreaded), dominancy (17,3 %) and important point index (96,1).




43

WETLAND PLANT AND MICROBE BIODIVERSITY


44

The Abundance of Arbuscular Mycorrhiza Infective Propagules

Under Galam Stand at Shallow Peat of South Kalimantan

Tri Wira Yuwati1, Annisa N.R. Rahmi2 and Badruzsaufari2

1Banjarbaru Environment and Forestry Research and Development Institute, 2Faculty of Mathematics and Natural Science, Lambung Mangkurat University

Abstract. Colonization of arbuscular mycorrhiza on plants have been reported to give benefit to plants

especially at extreme sites such as degraded peatland. Galam (Melaleuca cajuputi) is an indigenous peatland

species which grows on acidic condition. The number of arbuscular mycorrhiza infective propagules is

important to be determined in relation with the galam regeneration due to its offered benefits that supports

colonization. The aim of this research is to determine the abundance of arbuscular mycorrhiza infective

propagules under galam stand and to describe symbiotic forms of AMF colonization on the roots of galam.

The Most Probable Number (MPN) method, wet sieving and root staining from the modification of Vierherlig

et al., 1996 and the calculation of root’s mycorrhizal colonization by grid line technique were the methods

that were used in this research. The research used Complete Randomized Design (CRD) with a 5-fold factorial

patterns. The results of this study indicated a significant difference between the abundance of AMF under

galam stands at the depth of 0-15 cm and 15-30 cm respectively. The results of spores identification showed

4 genera of spores, namely: Glomus, Gigaspora, Scutellospora, and Acaulospora. The structure of root

colonizations were hyphae, spores, vesicles, and arbuscula.




45

Exploration of Fungi from Endophytic and Rhizospheric of

Galam Plant (Melaleuca cajuputi Powell.) that has a Potency to

Produce of Indole Acetic Acid (IAA)

Witiyasti Imaningsih 1,2, Safinah Surya Hakim3, Nadiya Dwi Rahayu1

1Department of Biology, Mathematics and Natural Sciences Faculty, Lambung Mangkurat University, Banjarbaru, South Kalimantan 2Microbiology Laboratory of Basic Laboratory Mathematics and Natural Sciences Faculty Lambung Mangkurat University, South

Kalimantan. 3Banjarbaru Environtment and Forestry Research and Development Institute, Banjarbaru-South Kalimantan

Abstract. Some types of fungi are known to have the ability to produce indole acetic acid (IAA). The fungi

could be isolated from the rhizosphere and plant tissues, especially from the rhizosphere and roots of Galam

Plant (Melaleuca cajuputi Powell.), which is known as able to grow dominantly on peatland. Therefore, the

aim of this study was to isolate and measure the potential of fungi from endophytic and rhizospheric of galam

plant (M. cajuputi Powell.) as a producer of IAA hormone, determine the types of interaction that occur and

their potential to increase the total IAA hormone produced. This research begins with isolation, purification,

isolate screening, analysis of IAA hormone production, data analysis, seed germination test and identification

of isolates. The result showed that the concentration of IAA produced by Penicillium sp. IRZ15 is 5.86 ± 0.47

μg.mL-1 to 8.46 ± 0.26 μg.mL-1 and Syncephalastrum sp. AG15 is 4.77 ± 0.44 μg.mL-1 to 8.77 ± 0.25 μg.mL-

1. Meanwhile, interactions between the rhizospheric fungi Penicillium sp. IRZ15 and endophytic fungi

Syncephalastrum sp. AG15 did not show significantly different concentration values with the use of fungi

isolates singly. Interaction between rhizospheric fungi Penicillium sp. IRZ15 and endophytic fungi

Syncephalastrum sp. AG15 able to produced IAA hormones ranging from 6.42 ± 0.34 μg.mL-1 to 9.19 ± 0.50

μg.mL-1. Interactions that occur between the Penicillium sp. IRZ15 and Syncephalastrum sp. AG15 is

suspected as the antibiose.



46

Analysis of Water Mimosa Leaves Proximate Content (Neptunia

oleracea and Neptunia plena) and its Potency in Improving

Spermatogenesis Activity and Spermatozoa Quality of Male Rats

Rusmiati1 and Sasi Gendro Sari1

1 Dept.of Biology Faculty of Matematic and Natural Sciences Lambung Mangkurat University, Banjarbaru, South Kalimantan, Indonesia

Abstract. Supan-Supan Laki (Neptunia plena) is one of the plants that includes in the water mimosa.

Information comparing the proximate nutritional content of Neptunia oleracea and Neptunia plena leaves is

not yet available. The plant leaves contain steroid, saponins, flavonoids, and alkaloids compounds which are

precursors of the hormone testosterone. One of the functions of testosterone hormone is to increase the activity

of spermatogenesis in the testicular seminiferous tubules and the quality of spermatozoa vas deferens. The

purpose of this study was to examine the potential of Neptunia plena leaves in enhancing spermatogenesis

activity and spermatozoa quality of male rats. This study used a Completely Randomized Design (CRD) with

an adverse control treatment (Na-CMC 0.5%), and the groups given ethanol extracts of Neptunia plena leaves

each at a dose of 70 mg/kg rats, 175 mg/kg rats, and 350 mg/kg rat body weight. Male rats have treated orally

2 mL / 200-250 grams bw for 14 days. On day 15, all rats killed by neck dislocation, dissected, testicular

organs taken, weighed, and microanatomy preparations made using paraffin method and Haematoxylin-Eosin

staining. In order to measure the quality of spermatozoa, the vas deferens are cut, put into a watch glass

containing 1 mL of physiological NaCl, pressed until the secretion/liquid comes out and suspended with 0.9%

NaCl, stirred until homogeneous. Then, the suspension of spermatozoa mixed with ethanol extract of Neptunia

plena leaves in a ratio of 1: 1. The mixture was then taken one drop to be placed on the slide, observing

viability, morphology, speed of movement, and spermatozoa morphology. The results showed that there were

significant differences in the proximate content of Neptunia oleracea and Neptunia plena leaves. Neptunia

plena leaves extract had the potential to increase spermatogenesis activity and spermatozoa quality of male

rats.



47

Chalcone Derivatives as Antibacterial Agents-A Review

Kamilia Mustikasari1,* and Uripto Trisno Santoso

1Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Lambung Mangkurat University Jl. A. Yani Km.35.8.

Banjarbaru, South Borneo

Abstract. Chalcone is a secondary metabolite compound in plants. Chalcones contain two aryl rings,

namely ring A and ring B which are connected to the α, β unsaturated ketones. Chalcone derivatives

are synthesized by various substituent groups in rings A and B and the types of rings. These variations

make chalcone and its derivatives have interesting bioactivity, one of which is antibacterial. This

review reports about chalcone-derived compounds which have antibacterial bioactivity, including

methoxy, ethoxy, hydroxy, and halogen groups in ring A or B. In addition, there are also variations

of ring A and ring B such as pyridine-chalcone, pyrrole-chalcone, tiophene-chalcone,

benzimidazolyl-chalcone and indole-thiophene-chalcone. We hope this review is useful for the

development of synthesis of organic compounds and the discovery of new drug design.




48

Cytotoxic Test of N-Hexane Extract of Mundar (Garcinia forbesii

King) Fruit Skin

Kholifatu Rosyidah 1,*, Muhammad Alfian Rizki1, Maria Dewi Astuti1 and Rodiansono Rodiansono1



Abstract. Research on cytotoxic test of n-hexane extract of mundar (Garcinia forbesii King) fruit skin has been

conducted. The cytotoxicity of n-hexane extract of mundar fruit skin was test by BSLT method againts A. salina

larvae to determine LC50 value. Weight of n-hexane extract obtained was 20.69 g from 405.64 g of dried mundar

fruit skin sample. The yield of n-hexane extract of mundar fruit skin was 5.10%. N-hexane extract of mundar

fruit skin is very toxic, the LC50 value obtained was 14.63 ppm.



49

Identification of Chemical Compounds from Nypa fruticans

Wurmb. Endosperm

Maria Dewi Astuti, Khairatun Nisa, Kamilia Mustikasari



Abstract. Identification of chemical compounds in nipah (Nypa fruticans Wurmb) endosperm has been done.

Extraction of chemical compounds in nipah endosperm has been done using maceration method with heating,

and followed with absorbsi-desorbsi on active carbon gived ethanol fraction. The result obtained was brownish

yellow fraction. Phytochemical screening test did on endosperm and ethanol fraction. Phytochemical screening

results showed that the nipah endosperm contains phenolic, tannin, flavonoid, and saponin compounds, while

the ethanol fraction of nipah endosperm did not contain alkaloid, flavonoid, steroid, triterpenoid, phenolic,

tannin, and saponin compounds. Based on UV-vis, IR and GCMS spectra, the ethanol fraction contains of

furfural compounds.



50

Potential of Leaves of Water Mimosa (Neptunia plena) Increasing

Spermatogenesis Activity Male Rat

Rusmiati¹*, Sasi Gendro Sari1, Chindy Rizka Febryandini Budyarti2

1Faculty of Mathematics and Natural Sciences University of Lambung Mangkurat Banjarbaru, 2Students of Biology Study Program Faculty of Mathematics and Natural Sciences Lambung Mangkurat University Banjarbaru

Abstract. Male supan-supan (Neptunia plena) is one example of a plant that includes water mimosa. The

leaves of this plant contain steroid compounds, saponins, flavonoids, and alkaloids which are precursors of

the hormone testosterone. One function of the hormone testosterone is to increase the activity of

spermatogenesis in the testicular seminiferous tubules. The purpose of this study was to examine the potential

of Neptunia plena leaves in increasing spermatogenesis activity. This study used a Completely Randomized

Design (CRD) with a negative control treatment (Na-CMC 0.5%) and the groups were given ethanol extracts

of Neptunia plena leaves each at a dose of 70 mg/kg body weight rat, 175 mg/kg body weight rat, and 350

mg/kg body weight rat. Male rat were treated orally 2 mL / 200-250 grams body weight for 14 days. On day

15, all rats were killed by neck dislocation, dissected, testicular organs were taken, weighed and

microanthomic preparations made using paraffin and Haematoxilin-Eosin staining. The parameter can be

observed from the spermatogenesis activity in testicles tubulus seminiferus, such as testicles weight, testicles

tubulus seminiferus diameter, and the number of spermatogenic cell layers (spermatogonium, spermatocyte,

spermatid, and spermatozoa). The result shows that Neptunia plena leaves extract were not influence the

weight of testicle and the diameter of the tubulus seminiferus of the testicle of male rat. Neptunia plena leaves

extract can increase the number of the spermatocyte cell layers and spermatozoa cell layers, so that they are

potential to increase spermatogenesis activity of male rat..




51

Cellulolytic Bacteria Isolated from Swamplands Activities and

their Effect on Rice Growth in Acid Sulfate Soils

Yuli Lestari1, Eni Maftu’ah1 and Wahida Annisa1

1Indonesian Swampland Agriculture Research Institute (ISARI), Banjarbaru, Indonesia

Abstract. Swamp land including acid sulphate soil is a unique ecosystem with distinctive chemical, physical

and biolgy characteristics. Biogeochemical processes in swamps are strongly influenced by microbe.

Microbes that are involved in the bigeochemical process are cellulolytic bacteria. This study aims to measure

the activity of cellulolytic bacteria isolated from swamplands and rice growth due to compost application with

cellulolytic bacterial decomposer in acid sulfate soils. The experiment carry out in laboratory and glasshouse

of Indonesian Swampland Agriculture Research Institute (ISARI), Banjarbaru, Indonesia at May-Novemper

2017. The experimental design used in the measurement of cellulolytic bacterial activity was a complete

randomized design arranged factorially with three replications. First factor was cellulolytic bacteria

inoculation (without inoculation/control, BS 1.6, BS 1.9, BS 2.2 amd BS 2.5), while second factor were

moisture (50%, 100% and 150%). The effect of compost application with cellulolytic bacterial decomposers

to rice growth arranged by factorial completely randomized design with 3 replications. First factor were

celulolytic bacteria inoculation (without inoculation/control, BS 1.6, BS 1.9 amd BS 2.2), while the second

factor were decomposition condition (saturated and flood 5 cm depth). The result showed that the ability of

cellulolytic bacteria to reduce C / N straw was not different. Only differences in moisture (water content)

affect the reducing C/N ratio of straw. The average C / N ratio of straw compost made with 50%, 100% and

150% water content is 35.59; 29.71 and 29.21. Application of compost made under saturated and inoculated

BS1.9 and BS2.2 can increase the number of tillers, while those inoculated BS1.6 and BS1.9 can increase the

rice shoot dry weight of Inpara 2. The suggest that cellulolytic bacterial inoculation can improve the quality

of compost so that the growth of rice is better.




52

In Vitro Testing of Nitrogen-Fixing and Synthesizes IAA

Hormones of Fungi Originated Peatlands Galam (Melaleuca

cajuputi Powell)

Siti Ko’idah1, Witiyasti Imaningsih1,2, Safinah Surya Hakim3

1Department of Biology, Faculty of Mathematics & Natural Science, University of Lambung Mangkurat, South Kalimantan, Indonesia 2Department of Biology, Faculty of Mathematics & Natural Science, University of Lambung Mangkurat, South Kalimantan, Indonesia 3Research and Development of Environment and Forestry, South Kalimantan, Indonesia

Abstract. South Kalimantan has a large enough peatland with high soil acidity, poor salinity, and availability

of nutrients slightly, causing only certain vegetation to survive. One of the plants that have a high tolerance

to peatland condition is Galam (Melaleuca cajuputi Powell). The study aims to test the mold of the rhizome

of the Galam plant (Melaleuca cajuputi Powell) of peat soil from its ability to hold N in vitro and measure its

ability to synthesize the hormone Indol Acetic Acid (IAA). The method used in this research is the isolation,

purification, and selection of rhizosphere mold derived from peat soil, and then the calculation of absorption

value by using a spectrophotometer for testing capabilities and synthesis of IAA hormones. Based on the

results of isolation and selection, obtained 3 isolates of non-pathogenic rhizosphere, which is potentially in

the order of N and synthesizes the hormone IAA, with IRh 11, IRh 25 and IRh 27 of isolates code. The IRh

isolates 11 and IRh 25 is the genus Monilia, and the IRh isolates 27 is a genus of Varicosporium. The results

of the measurement were obtained that all isolates were able to do N with the value of N detected infiltrate

isolates respectively of IRh 11 (2,124 μg/ml), IRh 25 (2,061 μg/ml) and IRh/27 isolates (2,074 μg/ml). The

ability to synthesize the IAA hormones on the treatment with additions and without the addition of tryptophan

respectively is IRh 11 (16.95 μg/ml-34.42 μg/ml), IRh 25 (17.89 μg/ml-34.95 μg/ml) and IRh 27 (17.68

μg/ml-53.26 μg/ml).



53

Anatomical Stucture and Terpenoid Content Zodia (Evodia suaveolens Scheff) Leaves

Evi Mintowati Kuntorini1*, Dewi Nofaliana1, Eny Dwi Pujawati1

1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Lambung Mangkurat. Jl. A. Yani Km 36

Banjarbaru, South Kalimantan, Indonesia. Tel./Fax. +62-511-4773112,

Abstract. Zodia (Evodia suaveolens Scheff) is member of Rutaceae contain terpenoids, triterpenoids,

alkaloids, flavonoids and xanthons which have anti-mosquito activity. This research aimed to observe the

anatomical structure, the location and distribution of terpenoid based on the leaves age. Anatomical slides preparation of leaves was made using the paraffin embedding method with safranin staining. The distribution

of terpenoid was analyzed by histochemical test. Leaf anatomical structure shows that the 3rd and 6th leaf

bifacial (dorsiventral) consisted of upper epidermis, mesophyll (palisade and sponge), collateral vascular

bundle, parenchyma midrib, abaxial epidermis and oil glands in mesophyll that are underneath both epidermis.

Diameter of oil glands with larger sizes were on the 6th leaf, whereas the density is not different in the 3rd and

6th leaves. The histochemical test showed that terpenoid was observed in the leaf vascular bundles, oil gland,

epidermis.




54

Morphological Characters and Nutrient Profiles of Water Mimosa (Neptunia oleracea and Neptunia

plena) from Banjar Wetland

Sasi Gendro Sari1*, Rusmiati1

1Department Biology, Faculty of Mathematics and Natural Sciences, Lambung Mangkurat University, Banjarbaru

Abstract. Two species of water mimosa found in Banjar wetlands were Neptunia oleracea and Neptunia

plena were categorized as tropical wetland plants. Those plants were rarely known by local people who lived

surrounding Banjar wetland areas. The research determined morphological characters and nutrient profiles

of two water mimosa. Plant characters were directly measured for length, width and diameter of plant organs.

Nutrient analysis were identified namely water percentage, protein, fat, ash, fiber content, carbohydrates, total of sugar and vitamin. Moreover, mineral contents such as iron, zinc, calcium, magnesium and phosphor.

The results showed that N. oleracea were floating with less branches compared to N. plena found near the

edge of wetlands through terrestrial. N. oleracea had higher nutrient contents such as protein, water, zinc,

antioxidant and vitamin C rather than N. plena. However, N. plena had more carbohydrates, fat, iron, calcium, magnesium, phosphor and calories compared to N. oleracea.




55

WETLAND BIODIVERSITY


56

Molecular Sexing in Lovebird Fischer (Agapornis fischeri) Using

Polymerase Chain Reaction Method

A D Argarini1, M Purwaningrum1, and A Haryanto1

1 Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta. Jl. Fauna 2

Karangmalang, Yogyakarta 55281, Indonesia.

Abstract. Lovebird is originally found in Africa and has spread to many countries. In Indonesia,

Agapornis fischeri is popular as a pet animal. Lovebird is monomorphic so it is difficult to see the difference

between male and female animals physically. Aves has ZW heterozygous sex chromosome in females and

ZZ homozygotes sex chromosome in males. These chromosomes are used for molecular sexing on aves. The

molecular method is an efficient method to determining gender. The molecular method is using Chromo-

Helicase- DNAbinding (CHD) gene amplification because in the CHD gene there are Z and W chromosomes

that found in Aves. The sample used for molecular sexing is the calamus feather. The number of samples are

11 and feathers which are used each for three to six feathers. The research was carried out by DNA isolation

from calamus feathers, DNA amplification using the Polymerase Chain Reaction (PCR) and DNA

electrophoresis. The results of DNA electrophoresis are visualized using UV light. The results of this

research were six male Lovebirds and five female Lovebirds. Differences between male and female is the

male will produce a single DNA band ZZ and the female produces two DNA bands ZW.




57

Genetic Diversity of Javan Deer (Rusa timorensis) Based on ATP Synthase F0 Subunit 6 (ATP6) Gene A Purwantoro1, S Arifah1, Yuriadi1, S Raharjo1, and S Gustari1

1Faculty of Veterinary Medicine, Universitas Gadjah Mada, FKH UGM, Jalan Fauna 2, Karangmalang, Yogyakarta 55281, Indonesia

Abstract. Javan deer (Rusa timorensis) is one of native deer in Indonesia. The population declines

significantly due to natural habitat degradation and poaching. As a consequence, IUCN classified it at

“vulnerable” status. Therefore, conservation in situ and ex situ is necessary. The study of genetic diversity is

performed to support for conservation purposes. This research was to study genetic diversity and kinship of

Javan deer with other deer based on ATP Synthase FO Subunit 6 (ATP6) gene and determine the possibility

as a genetic marker. Five mitochondrial DNA samples were isolated from the blood of Javan deer at Taman

Rusa UGM which originated from Papua, Indonesia. The DNA was amplified using PCR technique with

APRA6C3 forward and reverse primers. The amplification products of ATP6 gene were then sequenced. The

sequence data were aligned by Clustal W program and analyzed with MEGA 6.0 software by comparing with

other deer from Genbank data. The results show that specific sites of 40 nucleotides and 5 amino acids could

be used as a genetic marker to distinguish between Javan deer and comparator species. Furthermore, the

phylogram shows that the Javan deer has the closest kinship with Rusa timorensis of the Genbank data


mailto:[email protected]%20%09


58

Comparison of Goblet Cell Density in Small Intestine and Large Intestine Sunda Porcupine (Hystrix javanica)

Ariana, Teguh Budipitojo, Tri Wahyu Pangestiningsih, Irma Padeta, Hery Wijayanto

Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada Jalan Fauna No. 2, Karangmalang, Yogyakarta,

Indonesia, 55281

Abstract. Sunda porcupine are endemic animals in Indonesia. At present, the population of these animals has

begun to decline so it is necessary to document the histological data before these animals become extinct. This study aims to determine the density of goblet cells in parts of the intestine in sunda porcupine and then compares

the density of goblet cells between the parts of small intestine and large intestine of the animal. Three small and

large intestine sunda porcupine were taken as samples. The sample was fixed with Bouin's solution. Samples

are processed by the paraffin method before stained Periodic Acid Schiff. Comparison of goblet cell density between small intestine and large intestine, and comparison of goblet cell density between parts of small

intestine and large intestine of sunda porcupine were analyzed quantitatively. Analysis of the average of goblet

cell density between small intestine and large intestine of sunda porcupine showed a significant difference.

Anova analysis showed that there was a significant difference between the average of goblet cell densities between the duodenum, jejunum, ileum, cecum, colon and rectum. The post hoc test results showed that the

average goblet cell density in the duodenum, jejunum, ileum and cecum was significantly different with the

average of goblet cell density in the colon and rectum. The results showed that the density of goblet cell

increased on large intestine.

* Corresponding author:[email protected]


59

Locomotion Study of Bekantan (Nasalis larvatus)

Arvendi Rachma Jadi1, Amalia Rezeki2, Ariana 1, Dwi Liliek Kusindarta1, Hery Wijayanto1

1Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia

Abstract. Locomotion system in primate is developed and diverged following the pressure of the

environment. To survive, each species of primate will push their locomotors evolution to accommodate their

foraging activities. Generally, primate locomotion system is classified into vertical clinging and leaping (such

as tarsius), quadrupedalism (such as guenon), brachiation (such as gibbon), and bipedalism (human).

Classification is usually based on the intermembral index, the ratio between the arm and leg length in

percentage. The intermembral index of bekantan is about 90 on average; means the arm length is 90% of leg

length. Based on the intermembral index, bekantan's locomotion system is hindlimb dominated gait and

supposed to be quadrupedal terrestrial. However, in their natural habitat, bekantan spend their daily activities

mostly on the trees. Terrestrial and arboreal movements were observed during foraging. Arboreal movements

of bekantan are dominant where the forest canopy is remains good. On the other hand, in open areas and

where they have adapted with human existance, quadrupedal terrestrial behaviors will be dominant during

foraging. Further observation in variety areas are still needed to strengthen the type of locomotion system of

bekantan.




60

Molecular Identification of Gobiidae Fish of Kuala Lupak Estuary

Badruzsaufari1, A. Ramadhan1, Hidayaturrahmah1, A.J.Fiqri2, and H.B. Santoso1

1Biology Department of Lambung Mangkurat University, Banjarbaru, South Kalimantan 2Veterinary Center, Banjarbaru, South Kalimantan

Abstract. Fish belongs to subfamily of Gobiidae family that are able to live amphibiously on mud or brackish

water abundantly found in Kuala Lupak area of Barito River estuary. They have ecological important roles as

bioindicator and pollutant bioaccumulator at mangrove estuary. There are several gobiid fish known by the

local as glodok, timpakul, janjan, janjan sisik, janjan habang and timpunuk that are required to be identified

for scientific and conservation purposes. Identification of the fish mainly based on DNA sequences of

cytochrome-C oxidase subunit I (COI) genes that was supported with morphometric and meristic characters.

Five species of mudskippers were captured and their mitochondria COI gene DNA were successfully

amplified with VF2_t1, FishF2_t1, FishR2_t1, and FR1d_t1 cocktail primers. Subsequently, the PCR

products were sequenced by Sanger method using M13 primers. A phylogenetic tree was constructed based

on COI gene DNA sequences with Neighbor-Joining (NJ) method and Kimura-2 Parameter (K2P) model.

One species, i.e. glodok, has COI DNA sequence and morphometric and meristic characters which are

identical to those of Periophthalmodon schlosseri. Two other species, i.e. janjan and janjan sisik, are highly

similar to Pseudocryptes elongates and Parapocryptes serperaster, respectively. The third species, timpunuk

was found highly similar to Boleophtahlamus boddarti, however its DNA sequence and morphological

characters slightly different from those of B. boddarti. Morphometric and meristic characters of the last

species, timpakul, suggested it belongs to Periophthalmus chrysospilos but the gene DNA sequence of the

species was not available on database. The DNA sequence has less than 90 % similarity with other

Periophthalmus, such P. barbarus and P. magnuspinnatus. Phylogenetic tree analysis revealed that the

mudskippers was grouped into two clades, the first was species of Periophthalmodon and Periophthalmus,

and the second clade consisted of the species of Pseudocryptes, Parapocryptes, and Boleophtahlamus.




61

Morphology Identification of the Fruit Bat’s Tounge (Rousettus Amplexicaudatus) using Scanning Electron Microscope (SEM)

Geraldus Gunawan1, Golda Rani Saragih1, Hevi Wihadmadyatami1, Dwi Liliek Kusindarta1

1Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia

Abstract. Fruit-eater bats (Rousettus sp.) are from Suborder Megachiroptera keep their food in their oral

cavity to absorb the juice and discard the fruit’s waste. This habit evolves to modification of its digestive tract,

one of it is the tongue. There are many research about the dorsal surface of bat’s tongue but, the tongue

surfaces of Rousettus amplexicaudatus are still unexplored. Therefore, the aim of this study is to explored

the tongue surface of Rousettus amplexicaudatus. Macroscopic inspection showed that the tongue is divided

into three part, the apex, corpus, and radix. While Scanning Electron Microscope (SEM) study revealed the

papilas on the dorsal surface of the tongue are divided into 4 types, filiform papila, conical papila, fungigorm

papila, and circumvallate papilla. Furthermore, the filiform papilas are divided into 5 subtypes, small filiform,

scale like giant trifid filiform, leaf like filiform, cornflower filiform.



62

Morphological Study by Scanning Electron Microscopy (SEM) of

the Lingual Papillae in the Java Pipistrelle (Pipistrellus javanius)

Golda Saragih1, Geraldus Gunawan1, Dwi Liliek Kusindarta1, Hevi Wihadmadyatami1

1Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jalan Fauna No.2, Karangmalang, Yogyakarta,

Indonesia, 55281

Abstract. The bats have a very large number and ty,pes in Indonesia, which is 205 types or around 21% of

those in the world. Bats are included in the Chiroptera order which is divided into two suborders, namely

Megachiroptera (fruit bats) and Microchiroptera (insect bats). One of the insect bats is Pipistrellus javanicus,

known to the public as the Javanese Nighi bat. The purpose of this study was to determine the diversity,

distribution and morphology of the lingual papilla of Pipistrellus javanicus. The results of observations on

SEM were reported descriptively. The results of observations with SEM showed that the apex of the tongue

contained crown filiform, pronged filiform and fungiform papillae. In the corpus there are conical and

fungiform papillae. In the radix there are valatae, large filiform, small filiform and fungiform papillae.



63

Immunolocalization of Basic Fibroblast Growth Factor on Sunda

Porcupine’s Skin

Irma Padeta1, Ariana Ariana1, Teguh Budipitojo1

1Department of Anatomy Faculty of Veterinary Medicine Universitas Gadjah Mada Jl. Fauna No. 2 Karanglamang, Yogyakarta

Abstract. Introduction: Sunda porcupine is endemic animal which characterized by hollow spine on the

dorsal skin. Collagen is major protein synthesized by fibroblast which composed the dermis layer of skin.

Besides, fibroblast also synthesize basic fibroblast growth factor (b-FGF) which play the role in promoting

collagen production in various tissues. Objective: The objective of this study was to investigate the

immunolocalization of b-FGF and collagen distribution on Sunda porcupine’s skin. Methodology: Skin

samples were collected from two Sunda porcupine and were fixed in Bouin solution. The samples were

processed by paraffin method before stained with Mallory aniline blue (MAB) and immunohistochemistry

staining methods. Result: Collagen fibers on the dorsal skin were denser than on the ventral skin, especially

on the reticular layer of dermis. Then, dense collagen fibers were detected around the hair follicle on the

dorsal skin. Strong immunoreactivity of b-FGF was detected on epidermis on the dorsal skin, but on the

ventral skin only detected on stratum corneum. Immunoreactivity of bFGF was also detected in fibroblasts

and blood vessels on the dorsal and ventral skin. Besides, b-FGF was also detected on hair follicle lining

epithelium and sebaceous glands. Conclusion : The results suggested that b-FGF have a role in skin

development and immunolocalization of b-FGF promote the differences of collagen fibers density on the

dorsal and ventral skin.



64

Immunolocalization of 3β-Hydroxysteroid Dehydrogenase in Regeneration of Testicular Dysfunction by Mesenchymal Stem Cell-Conditioned Medium in Adult Male Wistar Rat

Linda Miftakhul Khasanah1, Teguh Budipitojo2, and Yuda Heru Fibrianto3

1Doctoral Student of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281 2Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281 3Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281

Abstract. Background: Testicular dysfunction is a major cause of sexual development disorders in male

animals. About 40-50% of infertility cases worldwide are caused by male factors. Testicular dysfunction can

occur due to toxic exposure, systemic diseases, oxidative stress or aging. Mesenchymal stem cell-conditioned

medium, commonly known as secretome, contains of growth factors and cytokines that can be found in culture

medium and have a role in regenerative process. Objective: Herein, we investigated the effect of mesenchymal

stem cell-conditioned medium in cisplatin-induced testicular dysfunction by examined the

immunolocalization of 3β-hydroxysteroid dehydrogenase (3β-HSD) as marker of steroidogenesis. Method:

All animal experiments were divided them into two groups: control group and secretome treated group (0,5

ml/kg body weight (BW)). Secretome treated group were injected with cisplatin 3 mg/kg BW, three times, to

induced the testicular dysfunction. The testicular tissues were processed by the paraffin method, cut to a

thickness of 5 µm, and histochemical staining of 3β-HSD and statistical analysis were performed. Results:

The 3β-HSD immunoreactivities were found only in Leydig cells and the intensity increased every week after

secretome injection. The number of immunoreactive cells significantly different between secretome treated

group one week after first injection (13,80 ± 3,692) and control group (40,00 ± 18,615) (p<0,05). There were

no differed significantly in secretome treated group second, third, and fourth week after injections compared

to control group (p>0,05). Conclusion: The result of this study showed that secretome promote

steroidogenesis after second injection of secretome on testicular dysfunction in rats.



65

Secretome Improve Androgen Binding Protein (ABP)

Production in Testicular Dysfunction Rats Induced by Cisplatin

Surya Agus Prihatno1

1Faculty of Veterinary Medicine, Gadjah Mada University

Abstract. Secretome is factors secreted by the Mesenchymal stem cells found in its culture media. Previous

studies reported that secretome has good effect in promote degenerative problem healing. One of degenerative

problem is testicular dysfunction. Testicular dysfunction causes an infertility and deficiency of ABP.

Determine the effect of secretome to regenerate the production of ABP and identify its distribution in testes

of testicular dysfunction rats due to cisplatin induction. Thirty six male adult rats were divided into three

groups, healthy group, secretome A (testicular dysfunction group treated with secretome at the dosage 0.2 ml

/ kg BW) and secretome B (testicular dysfunction group treated with secretome at the dosage 0.5 ml / kg BW).

Testicular dysfunction condition was induced by cisplatin administration at the dosage 3 mg / kg BW

intraperitoneally three times with three days interval. Rats in secretome group were injected with secretome

at the dosage 0.2 ml/kg BW and 0.5 ml/kg BW four times with seven days interval, respectively. Testes tissue

samples were collected every one week after secretome injection. Testes tissues were fixed with Bouin’s

solution for 24 hours, processed with paraffin method and stained with immunohistochemistry method for

anti-ABP primary antibody to determine the distribution of ABP in the testes. Immunohistochemical

preparations were examined with light microscope and the results were analyzed descriptively. One week

after first secretome injection in treated groups, the immunoreactivities of ABP were not detected. One week

after second and third secretome injection, in both dosage group of secretom, strong ABP immunoreactivities

were detected. One week after last secretome injection, strong ABP immunoreactivities were detected in high

dosage group of secretom. This result indicates that secretome injection at the dosage 0.2 or 0.5ml / kg BW

could improve the production of ABP in testicular dysfunction, which characterized by the presence of ABP

immunoreactivity in immunohistochemistry staining.



66

The structure of seminal vesicle gland of sunda porcupine

(Hystrix javanica)

Teguh Budipitojo1, Synthia Regita Noor Mahesty1, Yosua Kristian Adi1

1Faculty of Veterinary Medicine, Gadjah Mada University

Abstract. Sunda porcupine (Hystrix javanica) is one of the endemic rodentia in Indonesia. The aim of this

research was to investigate the topography and histological structure of sunda porcupine’s seminal vesicle

gland. The result of this study would be used for further research on reproduction system of Sunda Porcupine.

The samples of seminal vesicle gland were fixed in Bouin's solution for 24 hours, processed by using paraffin

method, and cut in 5 µm thickness. Tissues slide were stained with Hematoxylin Eosin to identified

histological structure of the glands. The stained tissue slides were observed by using light microscope.

Photomicrographs were using Optilab Image Viewer. The structure of the glands were analyzed descriptively.

The result of this research showed the topography of sunda porcupine’s seminal vesicle glands among others

male accessory genital organs. Seminal vesicle gland were lined by simplex columnar epithelium and were

classified as a serous gland. The topography and histological structure of seminal vesicle male accessory glands

of sunda porcupine (Hystrix javanica) has been identified.




67

Protein Diversity and Pylogenetic Relationship of Freshwater

Catfish (Siluroidei, Siluriformes) in South Kalimantan

Rani Sasmita1*, Mabrur1, Ummy Shaliha Aulia Rahmy1, Badruzsaufari1

1Department of Biology, Faculty of Mathematic and Natural Sciences, Universitas Lambung Mangkurat, Jl. A. Yani KM.36.00,

Banjarbaru, 70714, South Kalimantan, Indonesia.

Abstract. Protein profile, the result of SDS-PAGE analysis, is commonly used as marker for diversity

assessment of fish population. In this study, protein profiles was presented and characterized from the muscle

of seven Siluroidei species that is abundant in Barito river basin, South Kalimantan. The protein were

extracted from the fish muscle and characterized based on SDS-PAGE analysis. Protein diversity and

phylogenetic relationship was manifested from the protein profiles. The results showed that 35 protein bands

have been separated from Siluroidei population in South Kalimantan with the molecular weiht ranging from

28.33 to 170.23 kDa; 16 bands in Plotosus canius (28.33-170.23 kDa); 17 bands in Wallago leeri (28.33-

170.23 kDa); 18 bands in Mystus nemurus (28.33-170.23 kDa), Mystus negriceps (29.59-170.23 kDa), and

Clarias neuihofii (28.33-170.23 kDa); 19 bands in Pangasius polyuranodon (28.33-170.23 kDa); and 20

bands in Kryptopterus kryptopterus (28.33-170.23 kDa). On the protein level, the Siluroidei fish has high

polymorphism i.e. 85.71% with five monomorphic bands (30.96, 61.66, 97.73, 103.41, and 170.23 kDa). The

phylogenetic reconstruction also exhibited that the Siluroidei fish were grouped into two different clade and

has unique phylogenetic tree. This information would be useful for conservation program in the future.



68

Detection of Cymbidium Mosaic Virus of the Native Orchids

Germplasm in South Kalimantan, Indonesia Using

RT-PCR Method

Dindin Hidayatul Mursyidin1*, Ahmad Winarto Saputra1, Muhammad Aldy Hernanda1, Muhammad Irfan Makruf1

1Laboratory of Genetics and Molecular Biology, Faculty of Mathematic and Natural Sciences, Universitas Lambung Mangkurat, Jl. A.

Yani KM.36.00, Banjarbaru, 70714, South Kalimantan, Indonesia.

Abstract. Cymbidium mosaic virus (CyMV) is the most pathogenic viruses that infected the orchids. This

virus has caused the losses of many orchid cultivations throughout the world. In this study, we have detected

the virus infection from several native orchids of South Kalimantan, Indonesia, using the RT-PCR method.

The results show that one of the orchid samples was positively infected, namely Oncidium sp. The part size

of the viral genome that is detected is 530 bp. While this information is valuable for future orchid breeding

and conservation programs, further analysis is needed to employ.




69

Morphological Characterization of Two Populations of

Dorylaimid Nematodes from Curiak Island Bekantan Research

Station, South Kalimantan

Abdul Gafur1, Albert Oriya Gampung2

1Department of Biology, Faculty of Mathematic and Natural Sciences, Universitas Lambung Mangkurat, Jl. A. Yani KM.36.00,

Banjarbaru, 70714, South Kalimantan, Indonesia.

2Laboratory of Genetics and Molecular Biology, Faculty of Mathematic and Natural Sciences, Universitas Lambung Mangkurat, Jl. A.

Yani KM.36.00, Banjarbaru, 70714, South Kalimantan, Indonesia.

Abstract. A survey on soil nematodes of Bekantan Research Centre Sungai Curiak South Kalimantan has

been carried out as a part of faunal studies in the conservation area and a first step of the use of nematode

community as bioindicator of soil condition in the area. Nematodes recovered were fixed in formaldehyde and

mounted in glycerin as permanent slides. Morphological characters of specimens were then examined and measured using a compound microscope. Specimens belonging to two populations of dorylaimid nematodes

were found. Morphology of the specimens indicated that the two populations belong to genera Paralongidorus

and Xiphinema. However, species identification could not be made using available keys. Combination qualitative and quantitative (morphometric) characters suggested that the two populations were presumably

worth given new species status.



70

WILDLIFE BIOLOGY

AND MEDICINE


71

Recombinant Fussion (F) Protein Newcastle Disease Virus Expression from Escherichia coli plasmid Clone C2a using AccuRapid™ Protein Expression Kit

Ahmad Pandu Satria Wiratama1 and Aris Haryanto1

1Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada,Yogyakarta, Jl.Fauna 2

Karangmalang, Yogyakarta 55281, Indonesia

Abstract. Newcastle Disease Virus (NDV) is an infectious disease that infect many kinds of birds and

become a massive problem for poultry industry around the world especially in Indonesia. NDV

Vaccination is used to prevent the infection of NDV. NDV vaccine that used in Indonesia is a conventional

life vaccine from LaSota strain and B1 strain. These type of vaccine is 21%-23% genetically distinct with the

virus that spreaded in the environment. Thus the protection provided by the vaccine is no longer effective.

This is the reason why new kind of vaccine is needed to prevent the infection of NDV in Indonesia. From

the previous research, local isolate of NDV from Indonesia have been isolated. In the recent research, gene

that encoding the fussion protein that inserted in pBT7-N-His plasmid which isolated from E. coli clone C2a

will be expressed. The research is begin with detecting the present of pBT7-N-His plasmid in the sample

by using DNA electrophoresis. After that , the fussion protein is expressed by AccuRapid™ Protein

Expression Kit. After the expression is done, the r esearch continued with SDS-PAGE and western blott to

visualized the product of protein expression. The sample that used is the product of plasmid isolated from

E.coli C2a clone. From the electrophoresis of the sample, it was confirmed that the sample is containing

plasmid pBT7-N- His (4001 bp) which inserted by fussion protein gene (642 bp). After the protein is

expressed and visualized by SDS-PAGE and western blott, the product of protein is detected with molecular

weight of 25,6 kDa which assumed as the fussion protein. Key Words: Newcastle

Disease Virus, Vaccine, AccuRapid™ Protein Expression Kit.



72

Oriental Pharmacy and Experimental Medicine Ocimum sanctum Linn. Ethanolic Extract Inhibit the Angiogenesis on the Human Lung Adenocarcinoma (A549) cells

Ulayatul Kustiati1

1Faculty of Veterinary Medicine, Gajah Mada University, Yogyakarta-Indonesia

Abstract. Ocimum sanctum (OS) is tropical herbal plant which is easy to find and widely used as vegetable

food not only in Indonesia but also in the others tropical and sub-tropical country. In recent years, lung

adenocarcinoma was in top position as the cause of male cancer disease in Indonesia. Some researches,

revealed that the Ocimum sp. exhibit widely medical properties. Emerging data showing the extracts of

different species of Ocimum exhibiting the anti-tumor properties. Further studies on breast cancer and oral

cancer demonstrated anti-proliferatif effects after the treatment with Ocimum sp. extracts. However, the effect

of OS of Indonesian origin in the metastase of human alveolar pulmonary adenocarcinoma A549 cells still

unclear. Therefore, we aimed to investigate effects of ethanolic extract OS in A549 cell culture systems. The

tube formation assay revealed that OS ethanolic extract significantly inhibit the tube formation of A549 cells.

This condition also suppressed the expression of integrin αvβ3 as well as the MMP-2 and MMP-9. In

conclusion, our findings indicate that ethanolic extract OS prevent the angiogenesis of A549 cells, which may

result from the down regulated of αvβ3, MMP-2 and MMP-9 thus disrupt the cell migration and proliferation.

Taking together, ethanolic extract OS might be a good therapeutic candidate, hence the further understand of

its properties in the treatment of lung adenocarcinoma need to be done.



73

The Effect of Secretome Administration on Glucose Response in

Type 1 Diabetes Mellitus Rats

Dian Bekti Hadi Masithoh1 and Teguh Budipitojo2

1Doctoral student of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia 2Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia

Abstract. Type 1 Diabetes Mellitus (DMT1) is metabolic diseases where destruction of pancreatic β cells

leads to increased blood glucose levels. Secretom also called mesenchymal stem cell-conditioned medium

(MSC-CM), is a biological substance in cell culture media that has been used to grow stem cells. Secretome

contains many cytokines and growth factors and become a prospective drug to treat degenerative diseases.

The clinical application of secretome has been reported for several diseases, and the paracrine effects of

secretome may contribute to these beneficial effects. This study investigated the role of secretome to

maintanance the blood glucose level in DMT1 in rats. In this study, we were fourty two male rats with type 1

diabetes mellitus condition. Diabetic rats were divided into two group : diabetic group and group were injected

with 0,1 ml secretome as a treatment. Secretome were injected for four times with one week interval after

DM condition was reached. The blood glucose level in the rats was monitored on 1st until the 28th day using

a glucose oxidase-based commercial glucometer (Accu-Chek Active, Roche Diagnostic) and were evaluated

every week. The results were expressed as Means±SEM and P≤0.05 were considered statistically significant.

The statistical evaluation was carried out using SPSS, Data Editor, version 16.0. The blood glucose level

differed significantly for the second, third and fourth injection of secretome compared to the diabetic group

(p < 0.05). There was no significant difference between the first injection of secretome and diabetic group

(p > 0.05). The research clarify that there was decreasing the blood glucose level of diabetic rat group treated

with secretome. The presence result indicated the role of secretome in insulin-producing cells regeneration

that regulate the blood glucose level.



74

The Expression of Neuropeptide Y (NPY) on the Hipocampus of the In Vivo Model Neurodegeneration after Treatment of Ethanolic Extract Ocimum sanctum Linn.

Made Bagus Auriva M.1, Puspa Hening2, Aris Haryanto3, Sitarina Widyarini4, Yuda Heru Fibrianto5, Hevi

Wihadmadyatami6, Dwi Liliek Kusindarta6

1Post Graduate Student Faculty of Veterinary Medicine Universitas Gadjah Mada Yogyakarta,55281 2Post Graduate Student Research Center of Biotechnology, Universitas Gadjah Mada, Yogyakarta, 55281 3Department of Biochemistry, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281 4Department of Pathology, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281 5Department of Biochmeistry, Universitas Gadjah Mada, Yogyakarta, Indonesia, 5528 3Departement of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada Yogyakarta 55281

Abstract. Neurodegenerative disease is a disease that affects the central nervous system caused by the

progressive loss of the structure or function of neuron cells that affect memory, thinking ability, behavior and

response to daily activities. Neurodegenerative diseases have a huge impact on individuals not only on the

personality but also on the social and financial side. It is well known that administration of Ocimum sanctum

can improve the ability to remember by maintaining the stability of Cholineacetyltransferase (CHAT) enzyme

expression at a young age and restore ChAT expression in old age in both in vivo and in vitro models. In

addition to the neurotransmitter Acetylcholine (Ach) and ChAT enzime, the learning and remembering

mechanisms can not be separated from the role of Neuropeptida Y (NPY) as a powerful modulator of

neurogenesis. This study aims to determine the effect of Ocimum sanctum leaf extract on the distribution,

expression and concentration of NPY on the hippocampus of the in vivo model of neurodegenerative disease.

The study was planned to be carried out for 7 days and used 15 male Wistar rats (Rattus norvegicus albinus),

divided into 3 groups with 5 individuals each, consisting of control group (saline induced), treatment group

with TMT (Trimethyltin chloride) dose 8 mg / kg intraperitoneal (i.p), and TMT combination group with a

dose of 8 mg / kg i.p. and Ocimum sanctum leaf extract extract 100 mg / kg p.o. post-induction. On day 7

were sacrificed for brain sampling. Brain samples were made of paraffin block preparations and then cut with

8 μm thickness and followed by histologic staining using immunohistochemistry. The results were analyzed

qualitatively.



75

Ocimum sanctum Ethanolic Extract Inhibits Cell Viability and Induces Apoptosis on A549 Lung Cancer Carcinoma

Puspa Hening1, Teguh Triyono2, Supriatno3, Dwi Liliek Kusindarta4, Hevi Wihadmadyatami4

1Integrated Laboratory for Research and Testing, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia 2Department of Clinical Pathology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281 3Department of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281 4Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.

Abstract. Ocimum sanctum (OS) is tropical herbal plant which is easy to find and widely used as a vegetable

food in Indonesia. Recently, emerging data showing the extracts of different species of Ocimum exhibiting

the anti-tumor properties. However, the effect of OS of Indonesian origin in human alveolar pulmonary

adenocarcinoma A549 cells remain unclear. Therefore, we aimed to investigate effects of ethanolic extract

OS (EEOS) in A549 cell culture systems. Cell viability assays revealed that EEOS significantly decreased the

survival of A549 cell line. Morphological examination AO/EB indicated that EEOS induced the apoptosis of

A549 cells. n conclusion, our findings indicate that EEOS suppressed the viability of A549 cell and promoted

apoptosis on A549 cells. Taken together, EEOS might be a good therapeutic potential to further understand

its properties in the treatment of lung carcinoma.



76

Structural Recovery of Liver Failure Induced by Secretome

Teguh Budipitojo1, Dian Bekti Mashitoh1, and Neila Rahma Habibah1

1Department of Anatomy, Faculty of Veterinary Medicine, Gadjah Mada University Jl. Fauna No. 2 Karangmalang, Yogyakarta, Indonesia

55281

Abstract. Secretions from the human umbilical cord can increase the number and motility of spermatogenic

cells in mice undergoing testicular dysfunction due to cisplatin induction. Cisplatin as an anticancer drug can

cause hepatotoxic. The purpose of this study was to determine the effect of secretome on liver failure of mice

due to cisplatin induction. The study used 48 male mice which were divided into four groups, namely negative

control, positive control, 0.2 ml/kg body weight secretome treatment, and 0.5 ml/kg body weight secretome

treatment. Cisplatin dose 3 mg/kg BW was injected intraperitoneally three times at three-day intervals to the

positive control group and the treatment group. The positive control group was continued with 0.9%

physiological NaCl injection and the treatment group was injected with a secretome intraperitoneally once a

week at a dose of 0.2 ml/kg body weight and 0.5 ml/kg body weight for four weeks. Liver samples were

collected every one week after secretome administration, fixed with Bouin's solution, and processed by the

paraffin method. The microscope slide was stained with Hematoxylin Eosin. The results showed that the

secretome was able to regenerate cisplatin-induced hepatocytes. This can be seen from the structure of cell

histology that has improved in the form of proliferation of hepatocytes with normal shape and the reducing

the number of the failure cells. The best regenerative effect of hepatocytes was detectted in the secretome

injection treatment group with a dose of 0.5 ml / kg body weight in the second week.



77

The Expression of Serotonin in CA1 Hippocampal of Neurodegenerative Disease Rat Model Under Treatment Of Ethanolic Extract Ocimum sanctum Linn.

Ulayatul Kustiati1, Made Bagus A.M2, Dwi Liliek Kusindarta1, and Hevi Wihadmadyatami1

1Department of Anatomy, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia, 55281 2Integrated Laboratory for Research and Testing, Gadjah Mada University, Yogyakarta, Indonesia 55281

Abstract. Serotonergic system plays an important role in cognitive function especially in learning, memory,

and the focus of attention. Ocimum sanctum Linn.is a herbal plant that is found in all parts of Indonesia. The

combination of neuroprotective in basil leaves can improve memory (memory) ability, as a neurotropic agent,

and as a treatment for diseases related to memory ability. This study was aim to know the effect of Ocimum

sanctum Linn. ethanolic extract (OSE) on the neuron in the CA1 hippocampal area on the in vivo model of

Neurodegenerative Disease (NDD) Rat model.In this study two months old 21 male rats divided into three

groups, non-treated (Group A), NDD’s rats model pretreated with OSE followed induction for TMT on the

days of 7th (group B) and NDD’s rats model treated with OSE both on pre-TMT introduction for 7 days and

post-TMT induction for 21 days (group C). NDD rats sacrificed on day 7 th;14 th; and 21st. The brain tissue

samples analyses for neuronal density using Cresyl Violet staining and immunohistochemistry method for

serotonin.The brain samples. There is an increasing amount of the neuron, and serotonin expression in CA1

Hipocampal area of the NDD rats pre and post-treatment with OSE. Taking together, OSE has the potential

used to increase the expression of serotonin in the NDD rat model thus may act as neuroprotection agent and

neuroproliferation of the neurons



78

Discovery of Malaria Cases in Forest Workers in Teluk

Kepayang, Tanah Bumbu, South Kalimantan in 2018

Nita Rahayu1, Yuniarti Suryatinah1

1Tanah Bumbu Unit of Health Research and Development, National Institute of Health Research and Development (NIHRD), Ministry of Health of Indonesia, South Kalimantan, Indonesia, Loka Litbang Street, Tanah Bumbu Goverment Area, South Kalimantan

Abstract. Background: Tanah Bumbu Regency has an Annual Parasitic Incident (API) rate of 0.82 and in

2016 the number of APIs decreased to 0.48. However, there are cases of malaria that still occur in 5 (five)

locations with the category of High Case Incidence (HCI). Determinants in malaria cases in Tanah Bumbu

Regency are malaria parasites (Plasmodium falciparum, Plasmodium vivax, and mixtures), and forest

workers. Malaria which is more dominant in attacking the population in Teluk Kepayang District is malaria

vivax and mixture (P falciparum and P vivax). The aim of this study was to determine the dominant

plasmodium species that attacked the population. Method: Type of descriptive study with cross sectional

design in the location of endemic malaria villages in the category of HCI in Teluk Kepayang District in Tanah

Bumbu Regency. Results: A total of 773 residents were examined to detect active cases, mixed malaria (P

falciparum and P vivax) and P vivax were more dominant in adult men who worked as forest protectors and

miners in 91% of cases. Whereas malaria cases of P falciparum species are only found in adult women who

garden 9%. Conclusion: The discovery of malaria sufferers in Teluk Kepayang Regency received a total SPR

of 1%. Recommendation: For people in Teluk Kepayang Subdistrict to use insecticide-treated bed nets during

night sleep, and use repellents to avoid mosquito bites and use prophylactic drugs when going down to the

fields / forest to work.



79

Physiological Values, Conjunctiva Color and Feces Form as a

Health Parameter in Bekantan (Nasalis larvatus)

Soedarmanto Indarjulianto1*, Slamet Raharjo1, Agung Budiyanto2, Yosua Kristian Adi 2, Jumaryoto3, Amalia Rezeki4

1Department of Internal Medicine, Universitas Gadjah Mada 2Department of Reproduction, Universitas Gadjah Mada 3Graduate student of Sain Veteriner, Universitas Gadjah Mada

Jl. Fauna No. 2, Karangmalang, Depok, Sleman. 55281 Yogyakarta, Indonesia 4Sahabat Bekantan Indonesia Foundation, Banjarmasin, Kalimantan Selatan, Indonesia; Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Lambung Mangkurat, Banjarmasin, Kalimantan Selatan

Abstract. The important screening of the health status of animals, including bekantan (proboscis monkey),

begins by comparing the results of general examinations of patients with the physiological values of healthy

animals. This study aims to determine the physiological value of the bekantan including body temperature,

breathing frequency and pulse as well as the color of the mucous membrane, conjunctiva, and fecal conditions.

This study used 9 bekantan at Sahabat Bekantan Indonesia (SBI) foundation. All bekantan were inspected

their general condition, behavior, appetite, breathing frequency and fecal condition. Two samples were

examined for pulse frequency and mucous membranes, and 3 were examined for body temperature. The

inspection results showed that all bekantan were in good health conditions, indicated by good appetite and

drinking. A total of 8 animals showed elongated stool, soft and green while one bekantan feces was brown.

The breathing frequency ranged from 20 to 25 x / minute, the pulse frequency was 78 and 99 x / minute, body

temperature were 37.3 to 38.3 ℃ and the mucous menbranes were pale. This research data could then be used

as a reference for the initial screening of bekantan health status.



80

Blood Glucose Level of White Rats (Rattus norvegicus) after

Giving Catfish Biscuit (Pangasius hypopthalmus)

Hidayaturrahmah 1, Heri Budi Santoso 1, Ridha Aulia Rahmi 1, Dewi Kartikasari1

1Department of Biology, Faculty of Mathematic and Natural Sciences, Universitas Lambung Mangkurat, Jl. A. Yani KM.36.00, Banjarbaru,

70714, South Kalimantan, Indonesia.

Abstract. Glucose is important carbohydrate which is more absorbed into the bloodstream as glucose and

other sugar is changed into glucose in the liver. Blood glucose level is closely related to diabetes mellitus.

Diabetes mellitus is a disease that arises in a person which is indicated by the presence of blood glucose level

exceeding normal (hyperglycemia) due to the deficiency of the insulin hormone in the body. The design of

this research was using RAL with 24 male rats that divided into 6 treatments which were normal with the

giving of Na-CMC 0.5% 1mL/200 gram BB, negative with the giving of Na-CMC 0.5% 1mL/200 gram BB,

positive with the giving of glibenclamide of 0.45 mg/kg BB and 3 other treatments by giving catfish biscuit

(Formula A, formula B and formula C) with 4 replications. All treatments were injected with monohydrate

alloxan at a dose of 150 mg/kg BW, except for the normal treatment. The results showed that the formulation

of catfish biscuit can lower the blood glucose level of white rats. The composition of biscuit with formula C

has the highest decreasing percentage which was 80.20% to the blood glucose level of white rats.



81

Proapoptosis Effect of Root of Eurycoma Longifolia (Pasak

Bumi) on The Prostate Cancer: In Silico Analysis

Eka Yudha Rahman*1,2, Mulyohadi Ali3, Basuki Bambang Purnomo4, Nia Kania5

1Doctoral Program of Medical Science, Faculty of Medicine, University of Brawijaya Malang, Indonesia

2Department of Surgery, Urology Division, Faculty of Medicine, University of Lambung Mangkurat Banjarmasin, Indonesia

3Department of Pharmacology, Faculty of Medicine, University of Brawijaya Malang, Indonesia 4Department of Urology, Faculty of Medicine, University of Brawijaya Malang, Indonesia 5Department of Anatomical Pathology, Urology Division, Faculty of Medicine, University of Lambung Mangkurat Banjarmasin, Indonesia

Abstract.This study aimed to predict proapoptosis effect of E. longifolia active compounds on prostate cancer

by in silico analysis. Protein data such as BCL-2 (GI: 2506216), Caspase 3 (GI: 6978605), Caspase 8(GI: 11560103) dan data quassinoid (ID: 5459060 dan chantin (ID: 97176 ) were collected from genbank National

Center for Biotechnology Information (NCBI). Protein BCL-2 collected from NCBI compare with Protein

Data Bank (PDB) and UNIPROT. The docking process was carried out using software HEX 8.0. to compute

the binding affinity between ligands (active compounds of Pasak Bumi) and protein target. Each of active compound was docked to specific active site of protein target. All biomolecules were visualized using software

Discovery Studio 4.0. to generate the representative figure.The interaction between quassinoid and chantin was

strongest and stable against caspase-9, indicating that the active ingredient in E. longifolia triggered caspase-9 activity after activation of BH3 domains in Bcl-2 in prostate cancer. The low energy binding between

quassinoid and chantin with caspase-3 indicates the interaction between the active ingredients is not strong

with caspase-3. It is predicted that the active ingredient of E. longifolia does not directly interact with caspase-

3 in the apoptotic process of prostate cancer but caspase-3 activation is affected by caspase-9. E. longifolia active ingredients that are potentially used in the treatment of prostate cancer are quassinoid and chantin by

inducing apoptotic mechanisms via both extrinsic and intrinsic pathways. It was concluded that the

combination of active ingredients of E. longifolia that is quassinoid and chantin can be used as a strategy of

prostate cancer therapy both through extrinsic and intrinsic pathways



82

The Correlation of Femur Length with Body Length of Proboscis

Monkey

Ariana1, Irma Padeta1, Arvendi Rachma Jadi1, Hery Wijayanto1, Teguh Budipitojo1, Slamet Raharjo1, Amalia Rezeki2,

and Heri Budi Santoso3

1Department of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada

2Sahabat Bekantan Indonesia Foundation, Banjarmasin, South Kalimantan

3Biology Study Program, Faculty of Mathematic and Natural Science, Lambung Mangkurat University, South Kalimantan

Abstract. Proboscis monkey is endemic primate of Kalimantan Island. According to International

Union for Conservation of Nature (IUCN), proboscis monkey was classified as endangered species.

Anatomical data about proboscis monkey have not been available yet. As endangered and endemic

primate, morphology and morphometric data of proboscis monkey are important for forensic reference.

The purpose of this study was to investigate the correlation of femur length with body length of proboscis

monkey. Anatomical analyze used 3 proboscis monkeys from rehabilitation center, Sahabat Bekantan Indonesia, South Kalimantan. The femur length and body length were measured by digital caliper in

millimeter (mm). The value of showed that there was strong correlation between femur length and body

length of proboscis monkey. The body length of proboscis monkeys might be determined by femur

length with linear regresion y = 0.2862x + 347.85. There was positive correlation between femur length and body length of proboscis monkey.

* Corresponding author:[email protected]


83

ABSTRACTS

POSTER PRESENTATION


84

Increasing Rice Yield through Amelioration and Fertilization on

Medium Freshwater Swampland

Anna Hairani*, Muhammad Alwi and Khairil Anwar

Indonesian Swampland Agricultural Research Institute (ISARI) Jalan Kebun Karet, Loktabat Utara, Banjarbaru,

Kalimantan Selatan

Abstract. A big potential of freshwater swampland to overcome the land depreciation and the effects of

drought in some land typologies during climate anomalies such as El Nino. Some of freshwater swampland

has been utilized by farmers for food crops, horticulture, fisheries and livestock, but its utilization and

productivity are still under its potential and this needs to be improved. The research was conducted on 2.5 ha

of medium freshwater swampland at Hamayung village, Daha Utara district, Hulu Sungai Selatan regency in

dry season 2017. The experiment used a randomized block design with six treatments and three replications.

The treatments were: (1) Application of Pistia stratiotes + Biotara + Decision Support System (DSS)

fertilization system; (2) Application of Pistia stratiotes + Biotara + recommended fertilization system; (3)

Application without Pistia stratiotes + Biotara + DSS fertilization system, (4) Application without Pistia

stratiotes + Biotara + recommended fertilization system, (5) Only Pistia stratiotes and (6) Control. Soil

cultivation was carried out with minimum tillage. The rice variety was Inpara 2 with planting system of jajar

legowo 2: 1 (25 cm-50 cm) x 12.5 cm. The results showed that a combination treatment of 15 t in situ weed

of Pistia stratiotes/ha + 25 kg Biotara/ha + DSS fertilization system (50 kg urea/ha + 37 kg SP36/ha + 24 kg

KCl/ha) gave the highest yield (7.62 t/ha). This treatment increased yield by 10.28% compared to control

(6.91 t/ha) and was higher (52.40-69.33%) than those yielded by local farmers's cultivation (4.5-5.0 t/ha).




85

The Antibacterial Activity of Actinomycetes Against the Growth

of Streptococcus mutans and Lactobacillus acidophilus

Deby Kania Tri Putri1, Fadel Amirda2, Hilman Muzadi2, Amy Nindia Carabelly2, Isyana Erlita3, and Rahmiati4

1Departement of Biomedic Faculty of Dentistry Lambung Mangkurat University, Banjarmasin

2Departement of Biology Oral Faculty of Dentistry Lambung Mangkurat University, Banjarmasin

3Departement of Endodontics Faculty of Dentistry Lambung Mangkurat University, Banjarmasin

4Departement of Microbiology Parasitology Faculty of Medical Lambung Mangkurat University, Banjarmasin

Abstract. Antibacterial compounds are generally produced from many herbal ingredients, but antibacterials

may be produced from soil microbes, one of which is Actinomycetes. Actinomycetes is a soil bacterium

capable of producing secondary metabolites in the form of antibacterial compounds. The antibacterial

compound has the potential as an alternative antibacterial agent against the oral microbes. One of the oral

microbes that has a major role in the occurrence of caries is Streptococcus mutans and Lactobacillus

acidophilus. Objective: The aim if this study was to analyze the antibacterial activity of Actinomycetes against

the growth of S.mutans and L. acidophilus Method: Actinomycetes extracts at the concentration of 0.625%,

1.25%, 2.5%, 5%, 10%, 20%, and 40% were respectively exerted as treatment groups while 0.12%

Chlorhexidine Gluconate (CHX) and aquadest as control. The measurement of antibacterial activity is done

by looking at MIC and MBC. Results: One Way ANOVA and Post Hoc Dunnets T3 test MIC and MBC

values show p=0,000 (p <0.05). Actinomycetes extract at the concentration of 0.625% was able to inhibit the

growth of S.mutans and L.acidophilus which was determined as MIC and at the concentration of 40% as MBC

of S.mutans and at the concentration of 10% as MBC of L.acidophilus. Conclusion: There was an antibacterial

activity of Actinomycetes to the growth of S.mutans and L.acidophilus.




86

Effect of NPK Fertilizer and Microbial Consortium to Growth and

Production of Garlic (Allium Sativum L.)

Eti Heni Krestini 1,2, Ani Susilawati3, Catur Hermanto1

1Vegetable Research Institute, Jl. Tangkuban Perahu No. 517 Lembang Bandung

2Faculty of Agriculture, Padjadjaran University, Jl. Bandung Raya Sumedang KM 21, Jatinangor, Sumedang

3Indonesian Swamp Agriculture Research Institute (ISARI) Jl. Kebun Karet, Loktabat, Banjarbaru 70712, South Kalimantan

Abstract. Garlic productivity in Indonesia is relatively low due to ecological fitness, agronomic

performance, and pest losses. For the reason, the research was objected to study the effect of microbial

consortium and NPK fertilizer toward growth and production of garlic. This research was conducted at the

experimental field of Indonesian Vegetable Research Institute (IVEGRI) situated at 1.300 m asl in Lembang

– West Jawa – Indonesia from October 2018 until February 2019. Experiment was arrange in randomized

block design, consisted of 6 treatments, and 3 replications. The treatments were: 1) no microbial consortium

+ no NPK fertilizer (negative control), 2) no microbial consortium + 50% NPK fertilizer, 3). no microbial

consortium + 100% NPK recommendation, 4) application of microbial consortium + no NPK, 5) application

of microbial consortium + 50% of NPK recommendation, and 6). Application of microbial consortium +

100% of NPK rccomendation. The results showed that there was no significant effect of NPK fertilizer and

microbial consortium to growth and production of garlic. However, application of microbial consortium +

50% of NPK recomendation performed best on plant height and number of leaves, while application of

microbial consortium only resulted in best pseudostem growth and yield component of garlic.




87

Effectiveness of Microbial Consortium on Growth, Yield and

Intensity of Withered Disease (Fusarium oxysporum Schelecht)

on Garlic Plants

Eti Heni Krestini1,2, Ulya Rusmawati3 and Ani Susilawati4

1Vegetable Research Institute, Jl. Tangkuban Perahu No. 517 Lembang Bandung 2Faculty of Agriculture, Padjadjaran University, Jl. Bandung Raya Sumedang KM 21, Jatinangor, Sumedang 3Islam Nusantara Bandung University, Jl Sukarno Hatta no 530 Bandung 4Indonesian Swamp Agriculture Research Institute (ISARI) Jl. Kebun Karet, Loktabat, Banjarbaru 70712, South Kalimantan

Abstract. Garlic is one of the horticultural products that is further developed in Indonesia, this is done as

one of our efforts to increase the needs of meeting the needs of products that always rely on imports. The

productivity of our garlic is still low, below 20 tons / ha. To improve this efficiency, a technology

development is needed, one of which is by using a microbial consortium. Utilization of the microbial

consortium in garlic cultivation is still limited. This study examines the best benefits of the microbial

consortium for growth (height, number of leaves) and yield quality (tuber diameter and yield) as well as its

effect on the development of wilt disease caused by Fusarium oxysporum Schelecht. The research was

conducted at the Vegetable Crops Research Institute (Balitsa) in August to November 2018 using a

Randomized Block Design consisting of 6 preparations and 4 replications. The approved microbial

treatments were P0 (Control), P1 (12.5 g / plant), P2 (25 g / plant), P3 (50 g / plant), P4 (100 g / plant), and

P5 (150) gr / plant). The results showed that the use of 150 gr microbial consortium showed the highest

value for high plant parameters, while for the number of leaves, yield weight, tuber diameter did not show

differences between workmanship. The influence of the use of a microbial consortium is able to control

fusarium disease at 40 days after planting (HST) of 14.7-41.17 percent compared with control management.

.




88

The Influence of Tidal on Fe Distribution at Tidal Swamp Rice Farming in Barito River Area, South Kalimantan Indonesia

Mawardi1, Bambang Hendro Sunarminto,2 Benito Heru Purwanto,2 Putu Sudira,3 and Totok Gunawan4

1Indonesian Swampland Agriculture Research Institute, Jln Kebun Karet PO box 31 Banjarbaru. South Kalimantan, 70712, Indonesia

2Department of Soil Science, Faculty of Agriculture, Gadjah Mada University

3Department of Agriculture Engineering and Biosystem, Faculty of Agricultural Technology, Gadjah Mada University Flora Street no 1 Bulaksumur, Yogyakarta, 55581, Indonesia 4Department of Geographic Information Sciences, Faculty of Geography, Gadjah Mada University, Geografi Street no 1 Bulaksumur,

Yogyakarta, 55581, Indonesia

Abstract. Iron (Fe) poisoning causing problems on growth, the formation of saplings, and the filling of

grains. These problems lead to and plant death or decrease in productivity. The inhibition of growth and

production is increasingly higher in susceptible varieties. The distribution of iron concentration varies in

different regions and locations of swamp rice field. Various adverse effects of iron poisoning on rice have

been widely reported, but the spatial distribution of iron in tidal fields is still rare. Iron distribution in various

soil profile depths has not been widely investigated and data availability to avoid Fe poisoning in tidal swamps

has not been widely reported. Spatial distribution of Fe concentrations is needed as a reference for rice

cultivation in tidal swamps. This study has been conducted in November 2015 until August 2017 using survey

method in Barito River area, South Kalimantan. The use of thematic maps such tidal height difference (Δ),

tidal flood type and thickness of mud map. The results showed that spatial concentration of Fe is highest in

zone I which has the highest difference (Δ) of high tide maximum and low tide maximum. While on the soil

profile, the highest of Fe concentration is in layer 1 and the lowest concentration is in layer 3. Based on the

overflow type, the highest Fe concentration is type A and type B especially close to the estuary. To prevent

iron poisoning, rice seedlings should reach layer 2 (> 10 cm) and choose the safe zone that is zone II, III, or

IV to produce optimal rice production.

* Corresponding author: [email protected], [email protected]




89

The Potential Used of Swampland for Rice (Oryza sativa L.)

Production in Border Area for Agricultural Development

Niken Rani Wandansari1, Soemarno2

1Department of Sustainable Agricultural Extension, Politeknik Pembangunan Pertanian Malang

2Department of Soil Science, Faculty of Agriculture, Brawijaya University

Abstract. The utilization of swamp land in Indonesia has an important and strategic role for the development

of agriculture, especially in supporting National food security. This is due to the vast swamp area that has the

potential to be used as agricultural land, especially lowland rice. Swamp land is one of the suboptimal land

which has naturally low soil fertility. Therefore, it is important to study determining the potential use of

swamps land in the Lingga Regency, Kepulauan Riau to be used as lowland rice. This study was initially an

action study (in the first year) and the evaluation of the program (in the second year). The results show that:

(1) the main obstacles in the development of swamp land are soil pH (extremely acid) and soil nutrient status

which is low and (2) the rice yield (INPARA 5 variety) is relatively low, approximately 0.5- 2.5 tons/ha in

the first year and it decreased to 0.3-2.0 tons/ha in the second year. Rice production in swampland can be

developed through the application of location-specific technologies, especially the integration of water-

nutrient-plant management and the establishment of farmer institutions.




90

Water Management For Agriculture In Peatland

Vika Mayasari*and Wahida Annisa

Indonesian Swampland Agriculture Research Institute (ISARI)

Abstract. Water management in peatlands is a key factor in creating a sustainable peatland management

system. The main purpose of the drainage is to lower the ground water level, so as to create aerobic

conditions, at least to the depth of the roots of the cultivated plants, so that the plant's need for oxygen can

be met. The physical and chemical characteristics of peatlands affect their role in maintaining

environmental stability. Problems that arise in the development of swamps through reclamation of water

systems are related to the emergence of changes due to physical, chemical and biological processes. At first

the reclamation of swamps adopted the principle of how to drain the swamps. Peatlands become a source

of various environmental problems if the stability of peatlands is disturbed due to human intervention. The

drainage process is not accompanied by proper regulation and management of the water system resulting

in some of the environmental functions of the peatlands (including as carbon storage and regulating the

surrounding water system) will decrease.

* Corresponding author: [email protected]; [email protected]




91

Oriental Pharmacy and Experimental Medicine Ocimum sanctum

Linn. ethanolic extract inhibit the angiogenesis on the Human

Lung Adenocarcinoma (A549) cells

Ulayatul Kustiati1

1Faculty of Veterinary Medicine, Gajah Mada University, Yogyakarta-Indonesia

Abstract. Ocimum sanctum (OS) is tropical herbal plant which is easy to find and widely used as vegetable

food not only in Indonesia but also in the others tropical and sub-tropical country. In recent years, lung

adenocarcinoma was in top position as the cause of male cancer disease in Indonesia. Some researches,

revealed that the Ocimum sp. exhibit widely medical properties. Emerging data showing the extracts of

different species of Ocimum exhibiting the anti-tumor properties. Further studies on breast cancer and oral

cancer demonstrated anti-proliferatif effects after the treatment with Ocimum sp. extracts. However, the effect

of OS of Indonesian origin in the metastase of human alveolar pulmonary adenocarcinoma A549 cells still

unclear. Therefore, we aimed to investigate effects of ethanolic extract OS in A549 cell culture systems. The

tube formation assay revealed that OS ethanolic extract significantly inhibit the tube formation of A549 cells.

This condition also suppressed the expression of integrin αvβ3 as well as the MMP-2 and MMP-9. In

conclusion, our findings indicate that ethanolic extract OS prevent the angiogenesis of A549 cells, which may

result from the down regulated of αvβ3, MMP-2 and MMP-9 thus disrupt the cell migration and proliferation.

Taking together, ethanolic extract OS might be a good therapeutic candidate, hence the further understand of

its properties in the treatment of lung adenocarcinoma need to be done.




92

The Use of Fungal Endophyte Penicillum Citrinum in Tree

Seedling: Applicability and Limitation

Safinah Surya Hakim1 and Tri W Yuwati1

1Research and Enviromental Development and Forestry Center Banjarbaru Jl. Ahmad Yani Km. 28,7 Landasan Ulin Banjarbaru, South

Kalimantan 70721 phone (0511)4707872

Abstract. Penicillum citrinum is an endophyte fungi isolated from plant tissues that live in tropical peat land.

Various study reveals that endophytic fungi give advantages to plant health. Since 2015, series of experiments

were conducted to investigate the capability of Penicillum citrinum P3.10 as biofertilizer of plant tree in

nursery stage which are: pathogenicity test, in vitro assay, direct application to seeds, dosage test, and fertilizer

formulation. This paper presents a review from our research of Penicillum citrinum isolate P3.10 from

isolation stage to the biofertilizer product formulation. Research result showed that the application of

endophytic fungi to seedlings shows several positive responses. However, during the research process there

were also obstacles in the application of endophytic fungi to plants, which are: (a) difficulties in application

due to the very specific nature of endophytic fungi (b) storage (c) isolate viability (d) fungal isolate

maintenance (e)contamination and (f) difficulties in information transfer. Risk management were needed

during biofertilizer production. Hence, the utilization of biofertilizer made from endophyte fungi is applicable

and can be use not only in experimental scale but also in larger scale.




93

Impact of Land Reclamation On Acid Sulphate Soil And Its

Reclamation

Arthanur Rifqi Hidayat1

1Balai Penelitian Pertanian Lahan Rawa

Abstract. Land reclamation on acid sulphate soil is a process of improving acid sulphate soil to make them

suitable for a more productive use, such as increasing crop production. Reclamation efforts on acid sulphate

soils (land clearing, land management, water management system) had increased sulfidic material oxidation,

followed by soil acidification, rise of metal toxic solubility, and basic cation leaching. Mitigation efforts are

required to prevent these impacts such as proper water management, utilization of organic matter, adaptive

varieties, and optimized technology of fertilization. These mitigations must done carefully so that they have

minimum negative impact to soil and crop.




94

Eleocharis dulcis: A Practical Overview of Wetland- Based

Materials

Sunardi1,3, Wiwin Tyas Istikowati2

1Chemistry Department, Faculty of Mathematics and Natural Science, Lambung Mangkurat University Banjarbaru 70714 Indonesia

2Faculty of Forestry, Lambung Mangkurat University, Banjarbaru 70714 Indonesia

3Wetland-Based Materials Research Group, Lambung Mangkurat University, Banjarbaru 70714, Indonesia

Abstract. Eleocharis dulcis, known as purun tikus in Kalimantan, is a plant in the Cyperaceae family that

widely distributed in many places, particularly wetlands. These aquatic plants are undesirable plants, aggressive

and short life cycle with high reproductive capacity. Although considered as undesirable, the aquatic plants

can benefit human in many ways if their usage potentials are explored. The purpose of this study is to highlight

the important aspect of E. dulcis and to summarize the economic and commercial value of this treasure

produce in Kalimantan, Indonesia. E. dulcis is an important fiber source, and the major chemical components

are cellulose, lignin, and hemicellulose. Traditionally, the plant stems are dried, compacted, and plaited to

make various handicraft. E. dulcis also can be used as a raw material for the production of an enormous

range of functional materials (adsorbents, bioremediation agent, pulp and paper, medicine, and biofuel). It is

an abundant and low-cost source of wetland-based materials.




95

Evaluation of Growth Environment of Water Lilies (Nymphaea

pubescens Willd.) through Distribution Mapping in Non-tidal

Swamp, Hulu Sungai Utara Regency, South Kalimantan

Bakti Nur Ismuhajaroh1, Didik Indradewa2, Budiastuti Kurniasih3, Sri Nuryani Hidayah Utami4, and Rosalina Kumalawati5

1Postgraduate student in Agricultural Science majoring in Agronomy, the Faculty of Agriculture, UGM

2, 3, 4Teaching Staff at the Faculty of Agriculture, UGM

5Teaching Staff at the Department of Geography Education, ULM

Abstract. Water lilies is one of the aquatic plants found in various regions. The development of water lilies

has declined over time. Water lilies has many benefits as raw material for food and medicine. The distribution

of lotus plants has not been well identified. This study aims to find out the Mapping of the water lilies

Distribution for the Evaluation of the Growth Environment of water lilies in Teluk Sinar Village, Sungai

Pandan Sub-district, Hulu Sungai Utara Regency, South Kalimantan Province. This study was conducted in

April 2019 using data collection methods with primary and secondary data survey techniques. Primary survey

was through delineation of water lilies plant boundaries in the field in combination with semi-structured

interviews with selected respondents. The field measurements were also carried out to identify the variables

of the growth environment of water lilies. In addition, the interpretations of high resolution satellite imagery

and low resolution satellite imagery were used for spectral analysis. The variables of the growth environment

of lotus include the water depth, light penetration depth, water pH, and sediment pH. The mapping of water

lilies distribution was obtained from the overlay of several thematic maps, image interpretations, and field

measurements using the Arc View and Arc GIS software. The results showed that all study areas had the

distribution of water lilies plants. Water lilies could grow in the environments with a depth of swamp (35-121

cm), depth of light penetration (23-100 cm), water pH (6.25-8.37) and sediment pH (3.33-4.69).




96

Preliminary Prediction of Rice Planting for Planting Season

2019, 2020, and 2021 Based Water Balance in Freshwater

Swampland

Vika Mayasari*and Anna Hairani

Indonesian Swampland Agricultural Research Institute (ISARI) Jalan Kebun Karet, Loktabat Utara, Banjarbaru, Kalimantan Selatan

Abstract. Climate change that occurred in recent years causes a shift in the beginning of rice planting. This

happens because Indonesia's geographical location is at risk of being affected by changes in weather and

climate. Rice crop failure due to flooding or drought is the main cause of the low Cropping Index in the

freshwater swampland such as happened in Swampland Agro Science Park of Banjarbaru. Therefore, this

study was carried out to determine the preliminary prediction of the rice planting season for planting season

(MT) 2019, 2020 and 2021 based on water balance. As for what can be analyzed was the rainfall data as

input compared to evapotranspiration through the Hargreaves approach. Then the output obtained in the

form of net rate which used to measure rain every year. Meanwhile water requirement for rice was calculated

during the period of growth to harvest. After that the water balance simulation was performed from the

difference in water bubbles (net yield rate) with the water needs in the rice field. Based this water balance

simulation obtained that the beginning of rice planting for MT 2019 is May IV-June IV, MT 2020 is June

I- July I, and MT 2021 is June II-July II.




1

Empowerment of Batu Tanam Community toward Apiculture Village

Wiwin Tyas Istikowati1*, Sunardi2, Mochamad Arief Soendjoto1

1Faculty of Forestry, Lambung Mangkurat University, Banjarbaru 70714 Indonesia

2Chemistry Department, Faculty of Mathematics and Natural Science, Lambung Mangkurat University, Banjarbaru

70714 Indonesia

Abstract. The aim of this program is to make Batu Tanam as Apiculture village in the future. Batu Tanam village has some potential resources such as human resources, natural resources, infrastructure, and government support. Three main programs were implemented in this village to achieve the target. The main programs were training kelulut (Trigona itama) beekeeping to community, supplying beehives, and planting of some flowers as source of bee food. A beekeeper group were created and trained by an expert in beekeeping, 13 people joined in this group. In this program, were given 50 beehives to Batu Tanam beekeeper group. One person achieved 3 until 5 beehives, depend on their land support to kelulut bee. In nature, kelulut bee consume nectar from fruit flowers such as flowers of durian, coffee, cempedak (Artocarpus integer), and some others. However these trees does not bloom throughout the year therefore planting some type of flowers are needed. The type of flowers that suitable as kelulut bee food are calliandra, santos, widuri, sunflower, etc. From this program, some people from new beekeeper group already know and can maintain the kelulut beekeeping in their land. Batu Tanam communities already know the potential of kelulut bee and develop beekeeping program in their village.

Corresponding author: [email protected]



1

Genetic Variation of Proboscis Monkey (Nasalis larvatus) from Bakut island-South Kalimantan Based on Mitochondrial Cytochrome B Gene

Rini Widayanti1

1Faculty of Veterinary Medicine Universitas Gadjah Mada, Indonesia

Abstract. Aim: This study aimed to analyze the genetic variation of Nasalis larvatus from

Bakut island- South Kalimantan based on mitochondrial Cytochrome B gene sequences.

Materials and Methods : A total of four samples of Proboscis monkey were collected from

Bakut island- South Kalimantan, Indonesia. The DNA was isolated from the blood.

Mitochondrial DNA Cytochrome B was amplified using polymerase chain reaction (PCR)

with CytBF and CytBR primers. The PCR products were sequenced and continued to

analyze genetic variation using MEGA version 7.0 software. Results: Cytochrome b gene

sequencing obtained 1140 nucleotides encoding 380 amino acids. Sequenced Cytochrome

b gene fragments were aligned along with other primates from Genbank using ClustalW

program and genetic diversity among samples Proboscis monkey was analyzed using the

MEGA Version 7.0 software. Among all samples, there were substitution mutations at 5

nucleotide sites, and at 1 amino acid site. On the Phylogenetic based on amino acid

sequence results in three groups of Nasalis larvatus, group 1 consists of N. larvatus

(EU004476.1; KM889667.1; JF293094.1) and Proboscis monkey 2, group 2 consists of

Proboscis monkey 1, 3, and 4, and group 3 consisting of N. larvatus U62663.1 and

DQ355298.1. Conclusion : The results of Proboscis monkey from the Bakut Island based

on cytochrome B gene sequences, shows genetic variations at 5 nucleotide sites and 1

amino acid site. Phylogenetic Proboscis monkey with comparative data of N. larvatus from

genbank, Proboscis monkey 1, 3, and 4 from the island of Bakut are in different groups

from Proboscis monkey 2.



st International Conference on Tropical Wetland ...

Documents

Transcript of st International Conference on Tropical Wetland ...