spie.org/pw · customerservice@spie.org · TEL: +1 360 676 3290 · +1 888 504 8171 1

Contents7161A: Photonics in Dermatology and Plastic Surgery . . . . . . . .2

7161B: Urology: Diagnostics, Therapeutics, Robotics, Minimally Invasive, and Photodynamic Therapy . . . . . .11

7161C: Advanced Technology and Instrumentation in Otolaryngology: Lasers, Optics, Radio Frequency, and Related Technology . . . . . . . . . . . . . . . . . . . . . . . . . .19

7161D: Diagnostic and Therapeutic Applications of Light in Cardiology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23

7161E: Optical Techniques in Neurosurgery, Brain Imaging, and Neurobiology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28

7162: Lasers in Dentistry XV . . . . . . . . . . . . . . . . . . . . . . . . . . .38

7163: Ophthalmic Technologies XIX . . . . . . . . . . . . . . . . . . . . .46

7164: Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII . . . . . . . . . . . . . . . . . . . . . .62

7165: Mechanisms for Low-Light Therapy IV . . . . . . . . . . . . . .71

7166: Optics in Bone Biology and Diagnostics . . . . . . . . . . . .76

7167: Frontiers in Pathogen Detection: From Nanosensors to Systems . . . . . . . . . . . . . . . . . . . .81

7168: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIII . . . . . . . . .89

7169: Advanced Biomedical and Clinical Diagnostic Systems VII . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109

7170: Design and Quality for Biomedical Technologies II . . .123

7171: Multimodal Biomedical Imaging IV . . . . . . . . . . . . . . . .129

7172: Endoscopic Microscopy IV. . . . . . . . . . . . . . . . . . . . . . .139

7173: Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX . . . . . . . . . . . . . . . . . . . .145

7174: Optical Tomography and Spectroscopy of Tissue VIII . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .152

7175: Optical Interactions with Tissue and Cells XX . . . . . . .174

7176: Dynamics and Fluctuations in Biomedical Photonics VI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .185

7177: Photons Plus Ultrasound: Imaging and Sensing 2009 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .192

7178: Biophotonics and Immune Responses IV . . . . . . . . . . .213

7179: Optics in Tissue Engineering and Regenerative Medicine III . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .219

7180: Photons and Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . .224

7181: Energy-based Treatment of Tissue and Assessment V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232

7182: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII . . . . . . . . . . . . . . .239

7183: Multiphoton Microscopy in the Biomedical Sciences IX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256

7184: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .280

7185: Single Molecule Spectroscopy and Imaging II . . . . . . .291

7186: Optical Diagnostics and Sensing IX . . . . . . . . . . . . . . .302

7187: Biomedical Applications of Light Scattering III . . . . . .308

7188: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI . . . . . . . . . . . . . . . . . . . . . .320

7189: Colloidal Quantum Dots for Biomedical Applications IV. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .327

7190: Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications . . . . . .338

7191: Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .350

7192: Plasmonics in Biology and Medicine VI . . . . . . . . . . . .355

spie.org/pw · customerservice@spie.org · TEL: +1 360 676 3290 · +1 888 504 8171360

7192-36, Poster Session

Experimental study on the infl uence of the metal fi lm thickness on surface plasmon resonance biosensorsS. Ma, L. Liu, Y. He, J. Guo, Tsinghua Univ. (China)

Surface Plasmon resonance (SPR) is a promising technology in chemical and biology sensing. In a SPR sensor, a glass slide is coated with a metal fi lm, whose thickness , density and dielectric constant deadly affect the measurement sensitivity and precision in detection. The optimum thick-ness of gold fi lm in SPR sensors is 45nm without adhesive fi lm, according to calculations based on multilayer refection model cited in lots of papers. But experimental study on the optimum fi lm parameters is still lacking for the limitation of fi lm coating technology and high-precision thickness measurement. The optimum gold fi lm thickness observed in our detection is not 45nm, and the property of adhesive fi lm, which needed for enhanc-ing the adhesion of metal fi lm, affects the SPR responsive bandwidth and minimum value. In this paper, the strictly experiment study on the gold fi lm and adhesive fi lm of SPR sensors is described using high precision SPR detection system, X-ray diffraction and magnetron sputtering technology. Film density and structure are controlled by magnetron sputtering process and measured by Atomic Force Microscopy (AFM). Their infl uences on SPR are also discussed. We combine the experiment result with the multilayer refl ection theory to optimize the SPR calculation model.

7192-37, Poster Session

High-performance compact multi-channel sensor based on spectroscopy of surface plasmonsM. Piliarik, M. Vala, I. Tichy, J. Homola, Institute of Photonics and Electronics (Czech Republic)

Surface plasmon resonance (SPR) is a label-free biosensing technique with a large number of existing and potential applications in medical diagnostics, environmental monitoring, food safety and security. There is a continuous effort to bring detection capabilities of laboratory SPR systems to the fi eld. However, performance of current portable SPR biosensors is typically two orders of magnitude behind that of their laboratory counterparts. In this paper, we report a new compact multi-channel SPR sensor suitable for the use in the fi eld. The sensor utilizes special diffraction grating which performs coupling of light into the surface plasmon via one diffraction order and its simultaneous wavelength dispersion through another order of diffraction. This approach to spectroscopy of surface plasmons allows integration of excitation and interrogation of surface plasmons on a single plastic SPR chip produced by hot embossing. The resulting SPR sensor has a footprint of about 10x10 cm and incorporates optical bench, micro-fl uidics with six independent sensing channels, temperature stabilization module, and supporting electronics. In model refractometric experiments, it is demonstrated that the sensor is capable of resolving refractive index changes as small as 3E−7. This performance is comparable with the best laboratory SPR sensors.

7192-38, Poster Session

Detection of Avian infl uenza-DNA hybridization using surface plasmon resonance sensorS. Kim, S. H. Lee, Seoul National Univ. (Korea, Republic of) and Nano-Bioelectronics & Systems Research Ctr. (Korea, Republic of); K. Byun, Kyung Hee Univ. (Korea, Republic of); K. H. Yoon, T. H. Park, Seoul National Univ. (Korea, Republic of); M. L. Shuler, Cornell Univ. (United States); S. J. Kim, Seoul National Univ. (Korea, Republic of)

Avian infl uenza (AI) is an acute respiratory disease causing varying de-grees of clinical symptoms and illness. To date, several techniques have been developed for avian infl uenza virus (AIV) detection. However, most of these techniques involve painstaking and time-consuming laboratory procedures and also require expensive and huge equipments to detect the viral infection. In this study, we proposed an improved and simplifi ed surface plasmon resonance (SPR) biosensor for rapid, direct and accurate quantifi cation of AI-DNA. We implemented an SPR system optimized for hybridization reaction of AI-DNA with high sensitivity so that AI-DNA could be detected without the amplifi cation of real AI-DNA sample. In this study, we used oligonucleotide probe originated from avian infl uenza A virus (A/avian/NY/73-63-6/00(H7N2)) hemagglutinin sequences. We fi rst studied the immobilization of oligonucleotide probe onto an Au surface in order to compare the probe surface coverage and hybridization. The oligonucleotide probe with thiol was directly bound onto an Au surface for detecting both the oligonucleotide target and the real AI-DNA. The immobilized probe was suffi cient to induce the dose-dependent SPR wavelength shift. The possibil-ity of SPR-based biosensor for the detection of AI-DNA was demonstrated by the selective and sensitive hybridization reaction. We believe that the developed SPR biosensor is feasible for a real-time AI diagnosis as well as for the investigation of AIV infection.

7192-42, Poster Session

Plasmonics enhancement of metal nanospheres and nanospheroid linear chain systemsS. J. Norton, T. Vo-Dinh, Duke Univ. (United States)

We have developed a semi-analytical method for computing the plasmonics enhancement of the electric fi eld surrounding a fi nite linear chain of metal nanospheres and nanospheroids. The described method avoids the use of addition theorems, which are not applicable to spheroidal chains. The stan-dard treatment of chains or clusters of spheres generally use the spherical harmonic addition theorem to relate the multipole expansion coeffi cients between different spheres. Numeraical simulations are performed to illustrate the large fi eld enhancements that can occur in the nanoscale gaps between the silver nanoparticles arising from plasmon resonances.

7192-20, Session 4

Optimization and characterization of gold layered nanoparticles for multiplexed optical imagingM. A. McDonald, A. R. D. Hight Walker, National Institute of Standards and Technology (United States)

Metallic nanoparticles coupling strong surface plasmons with a light emit-ting molecule and/or a magnetic core have been developed for application in multiplexed optical imaging. Of interest to our work is the ability of the nanoparticles to serve as surface enhanced Raman spectroscopy (SERS) agents. We have optimized the chemical synthesis and characterized these two types of gold layered nanoparticles. In the fi rst case, the physico-chemical interaction between the adsorbate dye and the gold nanoparticle is probed. Rhodamine dye has been covalently linked to 5 nm gold nano-particle ‘seeds’. Gold layers were then iteratively deposited on the dye labeled seeds forming a rhodamine labeled gold core/gold shell inclusion nanoparticle. We will present data on the infl uence of dye concentration, gold outer shell thickness, nanoparticle size and shape on both the SERS and fl uorescence activity. Experimental evidence indicating the presence of dye in the nanoparticle core, utilizing X-ray photoelectric spectroscopy, fl uorescence/plasmon resonance energy transfer and quenching studies, will also be presented. Secondly, rhodamine dye was covalently linked to a magnetic, amine-functionalized, 5 nm iron oxide colloid. Gold layers were again iteratively deposited on the dye-labeled seeds. These fl uorescently labeled iron oxide core/gold shell nanoparticles could be ‘tuned’ during synthesis to display enhanced surface plasmon resonance or fl uorescence intensity. Characterization of the nanoparticles’ SERS, fl uorescent and mag-

Conference 7192: Plasmonics in Biology and Medicine VI