Sperm Transport in the Male Tract
Transcript of Sperm Transport in the Male Tract
Animal Science 434
Gamete Transport
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Sperm Transport in the Male TractFluid Flow Cilia
Fluid FlowMuscle Contractions
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Sperm Transport in the Male Tract
• No protein synthetic ability• addition or loss of plasma membrane proteins and lipids• redistribution of proteins and lipids within sperm• changes in lipid diffusion coefficients
Acquisition ofFertilizing Ability
and Motility
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Sperm Transport in the Male Tract
Addition of Decapacitation
Factor Addition of DF From Accessory Gland Secretions
Decapacitation Factor• protein, glycolipid and/or lipid• stabilizes the plasma membrane
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Site of EjaculationSite of ejaculation Semen Characteristics Species
Vagina slight coagulation of ejaculate human, rabbit
vagina semen with high sperm cattle, sheepconcentration
uterus voluminous, distention of cervix horse
cervix, uterus voluminous, retention of penis dog, pigduring copulation
uterus vagina plug rodents
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Sperm Loss From Female Tract
• retrograde flow• phagocytosis by neutrophils
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Experiment on Retrograde Flow in the Cow
Uterine Horn
Uterine Body
Cervix
Insemination at 3 different
spots
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perm
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Uterine Horn
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perm
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cervix
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perm
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Assume 10 million inseminated
Uterine Horn at 8 hr = 25% loss or 75% retained = 7.5 million sperm retained
Cervix at 8 hr = 60% loss or 40% retained = 4 million sperm retained
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Evolutionary Strategies for Dealing With Retrograde Flow of Semen• coagulation of semen or a plug• concentrated semen• semen deposited in uterus
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Sperm Loss From Female Tract
• retrograde flow• phagocytosis by neutrophils
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Neutrophile Infiltration of Uterus
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Phases of Sperm Transport• rapid
»15-30 min• sustained
»6 - 12 hr• oviductal
»at ovulation
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Evidence for Sustained Transport
Ligatures atUtero-tubal
Junction
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Effect of Oviduct Transection on Fertilization in Heifers
Interval from mating* to Oocytes Fertilizedtransection of UTJ
6 0%8 50%12 83%
* Mating was done at the start of estrus.
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Ovulation
OocyteCumulus Cells Follicular Fluid
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Cue for ovulation may effect isthmus to trigger
oviductal transport
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6-12 hr18-24 hr 1-2 hrLocation of Fertilizing Sperm
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Barriers To Sperm Transport
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Cervix
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Barriers To Sperm Transport
Uterotubal Junction
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Ovulation
OocyteCumulus Cells Follicular Fluid
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Fluid Flow
Factors Regulating Oocyte Transport in the Oviduct
Remains closed untilday 3 - 5 post ovulation
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Toward Uterus
TowardAmpulla
Total Fluid Production
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Factors Regulating Oocyte Transport in the Oviduct
Cilia
Fluid FlowMuscleContractions
No Contractions
Remains closed untilday 3 - 5 post ovulation
Site ofFertilization
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Now view the videopodcast entitled
View Video on Ovulation and Oocyte Transport
Found at:http://www.ansci.wisc.edu/jjp1/ansci_repro/lec/lec_16_gam
ete_tran/ovulation_oocyte_trans.html
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