1 From-Point Occlusion Culling From-Point Occlusion Culling Chapter 23.
Somatic cell count-based selection reduces susceptibility ... Rachel Rupp.pdf · Rg ....
Transcript of Somatic cell count-based selection reduces susceptibility ... Rachel Rupp.pdf · Rg ....
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Somatic cell count-based selection reduces susceptibility to negative energy balance during
early lactation in dairy sheep
J. Bouvier-Muller, C. Allain, D. Portes,R. Rupp, F. Enjalbert, G. Foucras
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2R. Rupp P61 – disease resistanceWCGALP 13/01/2018
Negative Energy Balance around parturition
NEFANon Esterified
Fatty Acids
BHBBeta-
hydroxybutyrate
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3R. Rupp P61 – disease resistanceWCGALP 13/01/2018
INRA La Fage, personnal
data
Higher risk of mastitis around parturition
Genetic correlations
•Rg BHB-mastitis = 0.48Koeck et al., (2014)
•Rg ketosis-mastitis = 0.17Zwald et al., (2004)
•Rg ketosis-mastitis = 0.26Heringstad et al., (2015)
Culling : Clinical mastitis Culling : other
Days in milk
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4R. Rupp P61 – disease resistanceWCGALP 13/01/2018
Link betweenenergy deficit and mastitis
in early lactation ?
Juliette Bouvier-MullerPhD Student 2014-2017INRA GenPhySE / ENVT
What are the consequences of energy shortage in early lactation in
dairy sheep selected for high and low mastitis resistance ?Métaprogramme
INRA
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5R. Rupp P61 – disease resistanceWCGALP 13/01/2018
Experimental design
RNA-seq blood samples (6 ewes per group)
Lambing
Daily feed intake, milk production traits, milk fatty acids, blood metabolites, live-weight, body condition score
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6R. Rupp P61 – disease resistanceWCGALP 13/01/2018
Metabolic profiles in the four groups of sheep (genetic line X diet)
Interaction genetic line X diet => High SCS ewes (susceptible to mastitis) are more susceptible to energy shortage
High SCS NEBHigh SCS PEB
Low SCS NEBLow SCS PEB
NEF A
Body
wei
ght l
oss (
%)
NEF
A
Days
DaysDays
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7R. Rupp P61 – disease resistanceWCGALP 13/01/2018
Data integration(sPLS – Discriminant Analysis)
BLOOD METABOLITES (n=48)Glucose, Insulin
NEFA, BHB
MILK FATTY ACIDS (n=48)
PRODUCTION TRAITS (n=48)Milk yields, Fat and Protein yields,
Fat and Protein contentsSCS
Body weight, body condition score
High SCS restricted (NEB)
High SCS control (PEB)
Low SCS restricted (NEB)
Low SCS control (PEB)
GENE EXPRESSION (n=24) RNA-seq
(20,017 genes)
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8R. Rupp P61 – disease resistanceWCGALP 13/01/2018
Data integration results : High level of adipose tissue mobilisation in High SCS ewes
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Production traitsFatty acidsMetabolites
High SCS NEBHigh SCS PEB
Low SCS NEBLow SCS PEB
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9R. Rupp P61 – disease resistanceWCGALP 13/01/2018
PhénotypesGene Expression
Data integration results : Increased Fatty acid oxydation in High SCS ewes
Glucose
Fatty acids
TCA
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10R. Rupp P61 – disease resistanceWCGALP 13/01/2018
Fat-to-protein ratio: a good predictor of NEB
Traits and genesCorrelation with fat to
protein ratioPDK4 0.62KLF11 0.76
SLC25A20 0.68CPT1A 0.77KLF10 0.67C16:1 0.73C17:0 0.80
anteiso C17:0 0.76iso C17:0 0.79
C18:0 0.77cis 9 C18:1 0.85
cis 11 C18:1_trans 15 C18:1 0.70
trans 9 C18:1 0.61Fat content 0.87
BHB 0.79NEFA 0.91
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11R. Rupp P61 – disease resistanceWCGALP 13/01/2018
Field data confirms a different energy status in sheep divergently selected for mastitis
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16,412 milk records (1025 ewes born between 2005 and 2016)Fa
t to
Pro
tein
Rat
io
Low SCS primiparousHigh SCS primiparous
Low SCS primiparousHigh SCS primiparous
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12R. Rupp P61 – disease resistanceWCGALP 13/01/2018
Conclusion and perspectives
High SCS sheep were more susceptible to NEB than Low SCS sheep in early lactation
SCS-based selection most probably limits metabolic troubles during this period
Fatty acid oxidation and glucose utilization in mitochondria are key mechanisms =>Functional studies needed
Pave the way for genetic studies on adaptation to energy shortage after lambing in dairy sheep (fat-to-protein ratio)
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Thank you for your attention
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Bouvier-Muller et al. JDS 99:480, 2016
Bouvier-Muller et al. Sci. Reports 7:2379, 2017
Bouvier-Muller et al. JDS 101:1, 2018