SNP marker protocol for tracking SNB resistance in flag leaf and … · 2019. 2. 20. · Page 3 of...

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SNP marker protocol for tracking SNB resistance in flag leaf and glumes of wheat

In part fulfilment of output 5 in GRDC funded project DAW00248

for improving SNB resistance in wheat breeding.

Prepared by Dr Michael Francki

Project Leader, DAW00248

Updated 31/12/2018

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Copyright © Western Australian Agriculture Authority and Grains Research and

Development Corporation, 2016

Permission to use copyright material

This document is protected by copyright law. Copyright of materials created by or for the

Department of Agriculture and Food resides with the Western Australian Agriculture

Authority established under the Biosecurity and Agriculture Management Act 2007. Apart

from any fair dealing or otherwise permitted under the Copyright Act 1968, no part may

be reproduced or reused for any commercial purposes whatsoever without prior written

permission of the Western Australian Agriculture Authority and Grains Research and

Development Corporation.

IMPORTANT DISCLAIMER

The Chief Executive Officer of the Department of Agriculture and Food, Western

Australia, the State of Western Australia, Western Australian Agriculture Authority and

Grains Research and Development Corporation accept no liability whatsoever by reason

of negligence or otherwise arising from the use or release of this information or any part

of it.

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Introduction

Stagonospora nodorum blotch (SNB) is an important fungal disease of wheat, caused by the

pathogen Stagonospora nodorum. Infection is prevalent in the leaves and glumes of host plants

and results in significant yield reduction if present at the critical time of grain filling. In Western

Australia, infection has been estimated to cause a 9% average loss in yield annually. In wet and

humid conditions, infection can occur over a wide range of temperatures with spores persisting

in stubble to act as source of infection in subsequent seasons.

Major advances in improving resistance to SNB in wheat have been limited by the complex

nature of the disease. Studies indicated resistance to SNB in seedlings, glumes and flag leaf is

controlled by independent genes. Resistance also appears to be inherited as quantitative trait

with the degree of resistance varying across cultivars. Identifying quantitative trait loci

associated with SNB will assist in tracking and combining traits for the improvement of SNB

resistance in wheat.

In this research funded by GRDC through project DAW00248, we have developed a protocol to

track QTLs associated with SNB resistance using DNA markers in flag leaf and glumes

identified from different donor sources. As SNB resistance is variable even between resistant

genotypes, a panel of SNP based markers spanning each QTL has been constructed for use to

track the SNB donor source.

This protocol provides information on chromosomal position of SNP associated with QTL and

necessary information to enable SNP marker to be implemented in a genotyping platform.

DAFWA has implemented the SNP marker information for germplasm development using the

Fluidigm EP1™ system as part of the GRDC funded project, DAW00248 and the information in

this protocol could be tailored for application using other suitable and appropriate genotyping

platforms.

DAFWA does not imply the approval of any genotyping platform or commercial product used in

this project to the exclusion of others that may be suitable.

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SNP panels

SNPs spanning each QTL were identified and converted to markers suitable for use on the

Fluidigm Biomark EP1 SNP analysis system. Each marker was validated against existing

mapping data and suitable markers used to construct a marker panel for each QTL.

For genotyping using the Fluidigm Biomark EP1 SNP system, four primers are required for the

analysis of each SNP. This includes

Two allele specific primers (one for each allele of the SNP).

One locus specific primer.

One specific target amplification (STA) primer. This primer is an enrichment primer

which is useful for complex genomes or low copy number targets.

For each of the SNPs in the SNB QTL panels, the sequences of the SNPs used are available in

Tables 1-13. The primer sequences used for marker genotyping via the Fluidigm Biomark EP1

system are available in Table 16.

Protocols for analysis using the Fluidigm Biomark EP1 genotyping system are available at

https://www.fluidigm.com/products/biomark-hd-system. Samples extracted using a range of

DNA extraction protocols were successfully analysed using this method. Irrespective of quality,

DNA concentrations greater than 100ug were found to yield the best results.

https://www.fluidigm.com/products/biomark-hd-system

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QTL mapping based on bi-parental mapping populations using

90K SNP data

In collaboration with the Department of Economic Development, Jobs, Transport and Resources

(DEDJTR), a 90K iSelect SNP wheat chip analysis was used to genotype three populations

segregating for SNB resistance. Four loci for flag leaf resistance to SNB on chromosomes1B,

2A, 2D and 5B together with 2 loci for glume resistance on chromosomes 2D and 4B were

identified.

Table: Summary of donor parents and populations used for QTL analysis for flag leaf and glume

resistance to SNB

Population QTL

location SNB resistance Figure

EGA Blanco/Millewa 1B Flag leaf Fig. 1

5B Flag Leaf Fig. 2

6HRWSN125/WAWHT2074 2D Flag Leaf Fig. 3

4B Glume Fig. 4

P92201D5-2 /P91193D1-10-2 2A Flag Leaf Fig. 5

2D Glume Fig. 6

SNP panel scoring for bi-parental mapping

Six SNP panels spanning the QTLs associated with SNB resistance were produced for

genotyping. A sample panel scoring is available in Table 14. The panels are designed to track

the resistant allele from a specific parental source of resistance. As indicated in Table 14, an

individual carrying the EGA Blanco source of SNB flag leaf resistance on chromosome 1B

would have alleles consistent with the yellow highlighted allele calls for this panel.

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Genome-wide association analysis using 90K SNP data

In collaboration with the Department of Economic Development, Jobs, Transport and Resources

(DEDJTR), a 90K iSelect SNP wheat chip analysis was used to genotype 232 spring wheat

individuals representing a wide array of germplasm. Ten marker trait associations (MTAs) with

moderate significance for flag leaf resistance to SNB were identified on chromosomes 1A, 1B,

4B, 5A, 5B, 6A and 7B. The MTA on chromosome 4B, as well as the two MTAs on chromosome

5B, co-locate with the QTLs identified through bi-parental mapping. An additional two MTAs with

a suggestive level of significance were identified on chromosomes 1A and 7A. Two MTAs were

identified for glume resistance on chromosomes 1B and 4B and co-locate with those MTAs

found for flag leaf resistance.

Table: Summary of GWAS for flag and glume resistance to SNB across three environments

from 2016-2018. QTLs in parenthesis indicate only a suggestive level of significance

Environment Year QTL location SNB

resistance Figure

Katanning 2016

5A-1, 5B-1 (1A-1, 1B-1,

6A)

Flag leaf Fig. 7

2017 1A-2 (1A-1,7A) Flag leaf Fig. 8

Northam

2016 1B-1, 6A, 7B Flag leaf Fig. 9

2017 (1A-1) Flag leaf Fig. 10

2018 4B, 5A-2 (5B-2) Flag leaf Fig. 11

Manjimup 2018 1B-2, 4B, 5A-2, 5B-2 Flag leaf Fig. 12

2018 1B-2, 4B Glume Fig. 13

SNP panel scoring for GWAS

Eight SNP panels targeting the MTAs identified in 2016 and 2017 were produced for selection

purposes. A sample panel tracking 1B-1 is available in Table 15. The panels are designed to

track the resistant allele from a specific parental source of resistance. The allele effect estimate

was also used to support the tracking of resistance. As indicated in Table 15, both ZJN12

Qno25 and 149ZIZ:13 have known sources of SNB resistance. Using the allele effect estimate

the allele present in 149ZIZ:13 was used for selection in the population.

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IWB55607 co-locates with IWB63360

IWB26464 co-locates with wPt-2751

IWB72737.2IWB117887.6IWB454378.1IWB237118.6

IWB6436812.0IWB5560712.5

wPt-894914.9wPt-826715.8wPt-709416.3

IWB2057018.9wPt-275119.9Xstm542a20.9

IWB4176923.9

IWB2950725.8

QSnl07.daw-1B

QSnl08.daw-1B

0

5

10

15

20

1B

EGA Blanco/Millewa

Fig 1. QTL analysis for flag leaf resistance in the double-haploid population 05Y001 (EGA

Blanco/Millewa) on chromosome 1B. QTL for flag leaf resistance evaluated in 2007 and

2008 are denoted QSnl07.daw-1B and QSnl08.daw-1B, respectively. Yellow shading

indicates SNP markers used for marker-assisted selection. Blue shading indicates an

alternative SNP marker is available for marker-assisted selection. Green shading indicates

SNP marker co-locating with DArT marker.

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wPt-3457240.9IWB55622241.4IWB15463241.9IWA5478242.5IWB12583243.4IWB22904244.3

Xfcp620248.5

IWA4103264.4

IWA1780267.3wPt-1733268.2IWB39412268.7IWA1797269.6IWB73666270.6IWB8906271.1

IWB3744273.7

VrnB1278.9

IWB25238283.0IWA7910284.5IWB27379285.1IWB46235286.1IWB40222287.0IWB11075287.9IWB62746288.9IWB56998289.4

QSnl07.daw-5B

QSnl08.daw-5B

0

5

10

15

20

5B

EGA Blanco/Millewa

Fig 2. QTL analysis for flag leaf resistance in the double-haploid population 05Y001

(EGA Blanco/Millewa) on chromosome 5B. QTL for flag leaf resistance evaluated in 2007

and 2008 are denoted QSnl07.daw-5B and QSnl08.daw-5B, respectively. Yellow shading

indicates SNP markers used for marker-assisted selection.

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IWB3544115.6

IWB6610719.4IWB4129521.3wPt-833023.1IWA815124.4IWB3872424.9IWB2809925.4IWB6583425.8IWB4639626.7IWB5390328.1

IWB761631.5IWB3868732.0

Xgwm15735.3

QSnl03.daw-2D

QSnl04.daw-2D

0

5

10

15

20

2D

6HRWSN125/WAWHT2074

Fig 3. QTL analysis for flag leaf resistance in the double-haploid population 98W816

(6HRWSN125/WAWHT2074) on chromosome 2D. QTL for flag leaf resistance

evaluated in 2003 and 2004 are denoted QSnl03.daw-2D and QSnl04.daw-2D,

respectively. Yellow shading indicates SNP markers used for marker-assisted

selection.

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IWB5607895.2

IWB7325898.0IWB7295598.4IWB472098.9IWB6993999.3IWB71675100.8IWB7612102.2IWB62190103.0wPt-3991105.3

wPt-7569108.4

Xbarc340112.7

IWA3325116.6

IWA4055118.4IWB28921119.3Xgwm495120.6

IWB41476125.8

QSng03.daw-4BQSng04.daw-4B

0

5

10

15

20

4B

6HRWSN125/ WAWHT2074

Fig 4. QTL analysis for glume resistance in the double-haploid population 98W816

(6HRWSN125/WAWHT2074) on chromosome 4B. QTL for glume resistance evaluated in

2003 and 2004 are denoted QSng03.daw-4B and QSng04.daw-4B, respectively. Yellow

shading indicates SNP markers used for marker-assisted selection. Blue shading

indicates an alternative SNP marker is available for marker-assisted selection.

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IWB41604 co-locates with wPt-3565


wPt-356516.8IWB1912317.0IWB2226817.7wPt-846418.2IWB2910318.4IWB1793718.7IWB1531518.9IWB779219.7IWB3244920.7wPt-244822.3wPt-832823.8IWA312224.4IWB5268726.1IWB144027.7IWB3565630.3IWA621932.6IWB2339733.1IWB5109933.6IWB3247434.5IWB920635.0IWB581335.6IWB964037.3IWB1207038.3IWB4251938.8Xbarc124b40.2IWB2875042.3IWB5727542.8IWB7299044.2IWB5787944.5

QSnl04.daw-2A

QSnl05.daw-2A

0

5

10

15

20

2A

P92201D5-2/P91193D1-10-2-92

Fig 5. QTL analysis for flag leaf resistance in the recombinant inbred line population

P9819RB1 (P92201D5-2/P91193D1-10-2-92) on chromosome 2A. QTL for flag leaf

resistance evaluated in 2004 and 2005 are denoted QSnl04.daw-2A and QSnl05.daw-

2A, respectively. Yellow shading indicates SNP markers used for marker-assisted

selection. Blue shading indicates an alternative SNP marker is available for marker-

assisted selection. Green shading indicates SNP marker co-locating with DArT marker.

Green shading indicates SNP marker co-locating with DArT marker.

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Xcfd50b0.0

IWB269544.2

IWB505327.9

IWB7379111.3IWB7379912.3IWB4531213.2IWB1198714.5IWB3528715.0IWB3145016.2IWB2112416.5Xgwm526a17.0IWB5343617.5IWB2579119.1

IWB908823.8QSng04.daw-2D

QSng05.daw-2D

QSng03.purE-2D

QSng03.purL-2D

QSng03.purV-2D

0

5

10

15

20

2D


P92201D5-2/P91193D1-10-2-92

Fig 6. QTL analysis for glume resistance in the recombinant inbred line population

P9819RB1 (P92201D5-2/P91193D1-10-2-92) on chromosome 2D. QTL for glume

resistance evaluated in 2003 and 2004 are denoted QSng03.daw-2D and QSng04.daw-

2D, respectively. Yellow shading indicates SNP markers used for marker-assisted

selection. Blue shading indicates an alternative SNP marker is available for marker-

assisted selection.

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A

B

Fig 7. Manhattan plot of GWAS for flag leaf resistance to SNB at Katanning 2016. (A)

flag leaf resistance (B) flag leaf resistance adjusted for heading date. Highly significant

threshold –log10 (P-value)>5.62. Moderately significant threshold –log10 (P-value)>4.00.

Suggestive significance –log10 (P-value)>3.00.

A

B

Fig 8. Manhattan plot of GWAS for flag leaf resistance to SNB at Katanning 2017. (A)




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A

B

Fig 9. Manhattan plot of GWAS for flag leaf resistance to SNB at Northam 2016. (A) flag

leaf resistance (B) flag leaf resistance adjusted for heading date. Highly significant



Fig 10. Manhattan plot of GWAS for flag leaf resistance to SNB at Northam 2017. (A)




A

B

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Fig 11. Manhattan plot of GWAS for flag leaf resistance to SNB at Northam 2018. (A)




A

B

Fig 12. Manhattan plot of GWAS for flag leaf resistance to SNB at Manjimup 2018. (A)




A

B

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Fig 13. Manhattan plot of GWAS for glume resistance to SNB at Manjimup 2018. (A)

glume resistance (B) glume resistance adjusted for heading date. Highly significant



A

B

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Table 1. Target sequences for the EGA Blanco source of flag leaf resistance on chromosome

1B. Target SNP shown in parenthesis.

SNP name Target sequence

IWB11788-1B GCGGCACTCCCATTAACCGGCACCTCAGCGGCAGCGACGGAAGGCGAGCTCGCGGCGCCGTTCACCTGCACCTCACCAGCAGCCTCGG[A/G]CGTGTCGCTAGACGGTTGCTTCGTAAGGGACTTCTGTTGACGGATGGCGGCCGCCGCTTCCGGCATGGCGACACC

IWB45437-1B TAGGAACGTGAGGCCACAACCTTTCTTGATGACTGAGATTCAGAGTCAGAGGTATTGATCCTTGATCGTCTTCA[A/C]GAGCTCTTCAACCCTCTTTATAATTTTAGGGTTCATCAGGTCCCTTGCCTGCCCAAGGAGAGTGCCCTCCCTGCTGCTATAATATGCATCGATTATAGCGCTTGCCC

IWB23711-1B CACCGAGGACACGCCGTCGAGCAGTGGGTATGCGAACGGCCATATGGCCACCGCTTCATCGCACGAACAAGATCATCCTTCTGAAGGAAGC[G/A]CGGGCAAGGCGGACGACAGGGCTACTACTGCCAACGTCAACCTAAAAATGGTCAAGATATCTGACAAGCTGATTGGTGTCTTCATGGTC

IWB55607-1B TTGTGCAGCCAACTGCAACCGAGAAAAGCGCAACTGACGTTATTTTTGGTGCGGAGTTGAGCCTGAGAGACACCA[A/G]TGTCTTGCAATAAGCTAAACAAGCAAGCCCAGAGTCATCGATGCATGAGCAGAAGCTTAAGGTGAGGTCAATCAGCGAGGAACAGTGAGATGAAAACACAGA

IWB63360-1B TTCTTGACAATAAATTAGTTGCAATAAAGAAGCCGATTAATGGTAGTGTGCTAGAGAGTGAACAATTTGCAAATGAAGTCATCATCCAATCT[A/C]GAGTCATTCACAAGAACATTGTCAGGCTCATTGGTTGTTGCCTTGAAGTTGATGCCCCTATGCTGGTCTACGAGTTTATCTCTCAAGGTAGCC

IWB26464-1B CACCGTTCAGCAGAACCGTCCTTGACGGGCATCCCTACCTGGTCTTCGAACCCAAGTTGGACAAATCCAATGTGCA[A/G]CAGAACATCGATTTCGTCAACAAAGAGATCGCCAGTGATCTCAGCACCATGACAAGGCCAGATGGCCCTCTCACTTTTGTTGGTAAGTCTGTGATAGTTA

IWB29507-1B ACACCACCAGCAGAAAAACCACAAAGCACCATAAAGCCTTCCATACAAGCAACCCCAAAGCCTTCTGC[G/A]CCCACACCACCAAGCTTTTCCAGTGAGCGCCCTTATGTCTGCCCCTACGAAGGCTGTGGGAAAGCCTACATCCATGGTTACAAAC

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Table 2. Target sequences for the EGA Blanco source of flag leaf resistance on chromosome 5B. Target SNP shown in parenthesis.


IWB12583-5B GATGCCGGCAATGGACGGTCCGGAGTCACACCGACCTTTTTACCAACAAAAAACATTTCAAAGTGTCAGGCCATCTGGTATCAACATCTGTT[G/A]AGTCTTATCGATGCCATGGTGCGGCAAAGCAAAGCAATGCTCACCTTATCTATGTCAGTATGCGCAGGGGTTTCGTAGCGCGCCACCGTCACGGCCA

IWB22904-5B TTCTTCTCACCTCGAGCTGCTATAGATATAGAGTCAGTGGACAGAGTCCAAAACAGAGTAACCTGCTGATCATCCAACAGGACACT[A/G]TTATTGTACATGTCACTGAGGCCTCCATCAACCACTTGAACCTTCCACCCCATCTTTGCTTCATACACCGACTGGTAGTAGACAAGATCAGGTG

IWA4103-5B ACCCCAACCTTCCACACTCCGCCTTCAAAAAAAGGAGCGGCGCGAACGAAGGCAGAGTCAGTCATCAATGGTTTTCGCTCTCATGCAACCGCAGGGCGAG[A/C]ACCGCGGCTTCCGGCTTTCCCGCCTTCGACCGTAGAGATAGTCCACCAGCTGCACCTTGGACCGCTGAACCACGGTTTTCGACGTCGTCTGCTCCTCCTC

IWA1780-5B TCTCATTGTATTCCAAGAAGGAGGACGAGTCCCAGCTTTTACAATTTCTCGACACCAAGTTTGGTAGTGGACAAGCAAATGGACCTGAGTTCTTTTACGA[A/G]CCCCAGTATGCTCTGCGTCTATGTCTCCAAGCAAGGAGGATGCGTGCTTGTGTTCGTATTTACAGCAYGATGTCCATGCATGAGGAAGCTGTGGCACTTG

IWB39412-5B TTTTCGGGAACGGTTTCAGCTCTTGGTAAGCACCGGGCCTGACGAACAGAATGGGTCCGTGTAGCGTCGGCATGGCTGGACACTCATCAAGCTGC[G/A]GCAAGCAGCTCGCGCATTTCTCCAGCAACAGGAGAAGATTTCAGAAGCCGCGGGTAACTCAAATCTACAATAGAGAATGATTTG

IWB73666-5B CATATGCCTGCTTATTATAATTCTATCATAAGAGGGGTCTCCACTATTTTGGTCTCATACAACAGTTGGAACGG[A/C]ACGAAAATGCACGCTAACCATCACCTAATCACTGATTTTCTGAAGAACAAGCTCAAATTTAGGGTATGTTTCTC

IWA7910-5B AAAGACCAACCTAAGGCAAAATCCGAACGAGTGAAAAACCTCCTTTTCTCAACTTCTGACTGCACTTCTGTGTGCAACAGATGACTAAAACCCGTCTTGC[A/G]CTTCTGCTAGGTTGATAGATTACAAAAATGACGACGACCAAAACTGAGATCAAACTGACTAGAGTACATCAATCCACCAATGGCTGGAAGCAATGTTTAA

IWB27379-5B ACAGATTAATCCCAATAAAAATAACCCATACATTTGACTGTTGCAACTCTAATACTGTTGGATCTTAGGACTGCCCAATGTAGACAATTCACTT[G/A]CCCTGTTACAGAAATGGTGATATGCATGTCGCCACATTCATCAACCACTATCACTGAAGTATAAACAACAGTGTGCT

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Table 3. Target sequences for the 6HRWSN125 source of flag leaf resistance on chromosome 2D. Target SNP shown in parenthesis.


IWB35441-2D CCCAGAGGCACATGCACGGGCCTATGCCTCGGACACCATCTCAGCCACCTCGCCGGGCATGAACAGGACTCGCCGGGGATTCACTAGAAGAAAGAGGTGC[A/C]GAGGACAACCAACATACATAGTCCGGCACAGCCTCTCATTCTCCATGTCACATGAACGTCAAGCATATGCCTTGGGACTTTCATCCCGGTCGCCTCGGCC

IWB66107-2D

TCATGGTATGAGTAAATCAAAGGAAAAGTGAGCCATACCATTTTATTGTTGGACTTGGATTTGCTATTGCTGTGGTTATTGTTGGTCGGAG[C/A]CTTGGTTTTACTCTTCCCACTAACAACATCAAAAATCGTGGGAAGCTCATTAATCATACCAAACAGGCGCTTCCTGCATTAAGATAACAGCAAAGCATCAGTAACAACACATCAGGTCAACAG

IWB41295-2D ATCCTTACACTGGGAGATATCTGATTTTCTCCATCGCACTGAAAAGAATATTCCAAGTGAGGATGATCCGGTCAAGACAGGT[A/G]TTCATAGGCAGATAATTGAGATGAAGGATTTGCTTAAAATGGAAAGAAACGAATGCGAGGTGACGAAATTAGATTTTT

IWA8151-2D AGGATGTGGACCTTATGAAGACCTATATATCGAAGATTCAGCAGTTGGAGGCTGAAGTAACACGGCAGAAGTTCACCACTGCCTGTAGAAATGGTTTG[T/C]ATGATCGACTTGCACTGGATAAGGGCATGCTTCTAGACGATCTAGGTTCAGGTTGTGAAGTAGGAACGCTCGAAGTGTCAAGT

IWB28099-2D ATGTCGTCCGCTTCAGATCCGAATGACACCAGGAATCGAGTCTTCAGGATTCTTGAAATGTTCGACAAATGTGCC[A/G]CAAACTGCAGTACTCCTGATGAGGCGCATAAAATGCGTGAGATGAYTRKAGCATCCGCAGTTGCTTCAT

IWB46396-2D ATTTTTCTGGGATTTGAAAACTGAATTGTCCTTTAAAAAATACAGCTGATGTTCAAGCTTACCCTGATGAGTACAGTCCTGACTGGGGTCGGATG[A/G]GTTTCTTAGTAGACATTTTGAGGCCAACCCCTGGAGAGGGGCCTGCATGGTACCATCTGTTGAAAAAAGCCAGTGGAGGTTGCAATGATCTTATGTATAGTGGG

IWB7616-2D GCCAACCGTCGTGGTGATGGCTCTGTTGCTGGCCACGTTGATCGCCCTCTCCTTGGCGCACAAGGAGCACCAGCAC[G/A]CGCCAACTCAAGCACCGAGCCCCGCTCACCATCATGGCCATCTTGCCCCGGCGCCAAGCCATTATCA

IWB38687-2D CACATGCATGGGTCTTGCAAACTTTTTTCCATTCCTTTAAGGAGAGTTAGAATAATACTGAAACCACATGCCGCTG[G/A]AGCTGGTGACAGTAAAATATGCTACTACTGTGACAGTTAAAAGGAAAAACTAGTTGCCACATGGCTTGTGCAGAGCTGCACAATTGACATGCTTT

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Table 4. Target sequences for the 6HRWSN125 source of glume resistance on chromosome 4B. Target SNP shown in parenthesis.


IWB56078-4B CCATATTTAAAACTGTGCTGCAAAAGCTACAAAACCTCGATGTTACAAACCGTCTCCAACCAGCCTTATTCTGTGAGTAAAACAGACGT[A/G]CACACTTTTTTGGACACTATAAAAGACACCACCTCCACGTCTAAATGACTCGGGTCACAATGT

IWB73258-4B CAGGAAACGTAAGCCGCTATTGGTTAGATACCTGCAAATGATTAATTGTTAAAGACTACTTTGGGAACAATCACTCTGAGCAATAAAC[A/G]GTAATAAACAGAGAACAAAAAGGAACAAAACAAAGCCAGGTTTTTTCACCTAAAAGGAATTGTCAAATGCAGCGATAGCATGTTTGCATTTTTTT

IWB72955-4B ACCAATTACATTATCTCCACACTTGTGCTTCTATTCTTTGAACATGGACTGTTTACATGTGGCACACATTGTTG[G/A]CGCTTTTCAAATAAGCTAGCAATAGACCGCCTTTTCCTCATGGACCCGTTGCGCCAAATGGCAGAGACCCGCTGAATCCATGTTCACATTGAAAAGAGAATCATGGTTTA

IWB4720-4B TTGGAGGAGACTCGAAGCCTAATCTTCCGCCTCGATCTCTCCCGCCGCAGGAGAGATCCCTCTCAGCCTCGGCTCCAATCCGCGACTCGACT[A/C]GAAGCTCCAGCTCCGCCGCCGCCCCCCGCCTCCGATGGATCCGAAGCTGGTGGAGGTGACGCAGCTGTTCGACCGCTTCAAGGCGGCCT

IWA1846-4B TGACCGGCGCCGCCGGGATCTCCTTACAGGGCTGCGACTCCTTCACCTTCTGGAACAAGACCCGGCCATCCTTGACATCCCACTCGGGGTGTTCCTCCGC[A/G]ATGTACTGTTCCAGTTCCTTGTCGGAGCTGAACTTAAACATCTGCTTCGCGTCGTTTATTGACAGGTAATCATATCCCTTCTCGCTGCACCCAGCTATCT

IWB71675-4B AGCTCGTGTCGTCGCGTCGTGAGAGACAGGGAGAAATCTTCTGGTGTGTGGAGGTAGGGGTCAGCAGCCCGGGGATTTCGCGGG[G/A]ATCGGGCCGAGAAATCCCTTTTCCCCGATGACACCAAATGGGCCGCTGGAAAAACCGGGTTGAAGATGC

IWA3325-4B TGGTACTGTGCATTTATTTCGTTCAATCTCTCCCTGTGCCTAGTATTTTCAGCATCCTCTGCATCATTTAGTTCCTTGGATAGGACCACGATGTCTTCAC[A/G]AAAACTCTGCTCTAGACTTTCAAATGTACGAGGAAGATTATACTCATCGTGCCTTGCTTCAATCTTATCGGTACGAGGCTGGTCCCTCGATTCCTTTCTT

IWA4055-4B ATGCTAAACCCTCTCAGGAAAGTATGTTGCCTAGCAGATTGTTTGGAAGCATCAAACAGATGCACTGCGTGCTATGAAAGAATGGTTCTTCCCCAACCCA[A/C]TAAAGCGGGGCTGGACAAGGGCATTATATATACAAGGCCGGCCGGCATGCTGTCGTAGGCATCAACTGCTACGCCGGGTCGTTCATCGGTGAAATGAAGA

IWB28921-4B ATGTACCATGCTGGGTGGAGGGGACACTGACTCGGCGGCCAGTCCTCGGCTGATGTAGCGTCCTGTCGTCGCATGACGCCGTTGTGCGCCGTCG[A/G]AGGACGCGTCCGGCTAGTAAGACACGTGGTGCGTCCTGCCATTTACGATGAGTCAGACTGTCGAAGAGGAG

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Table 5. Target sequences for the P92201D5-2 source of flag leaf resistance on chromosome 2A. Target SNP shown in parenthesis.


IWB41604-2A CGAGAGCGGCGACGAGGAGCTCACCGTGCTGCCCAGGCACACCAAGGTTGTCGTCACCGGTAACAACCG[A/G]ACAAAGTCCGTGCTGGTCGGCCTGCAGGGTGTGGTCAAGAAGGCTGTTGGCCTTGGAGGATGGCACTGGCTGGTCA

IWB29103-2A CAGATTATATGGCCAGGGTACAGGAGATTGCCAATGCTGGCTATAAGATGAACTTTGGCAATCCAGCTATGCAGCAGCAGCT[A/G]CTTGCAAACGCAAACTGGCCCTATGGGATGGCTGGTAGATATGGTATGCAATCTTCTGCTTGGCCATTCGCCGGCAACAGCACAAATCAGTTTCAAGGTGTTCCAAAG

IWA3122-2A TCCCCATGCACAGGCTCATCCACGGCATCATCACGTAGTATGAGTAGGCCCATAGGAGCCGAGCGACGATGGCCAGGGAGAGCCCTGCGAACAGGTACCC[A/G]CTGTAGGCTACCAGGTCGAGCAGCGGCACCTCGCCACCGCCCATCGAGTAGAGGAGCCCTTTCAGGAACACGATCTGTAGGCCCCATCCGATCAGCCCTC

IWB9206-2A AGGTGATAATTCGCAGCCTGAAGAATCCGATGCAGTCAGTTAAGATTAATGTTGTATTGGCTACGTTGCTATACCAAGCACC[G/A]TCTGGCATCGCAATTTTCTGTTTTGATGGAGATTACCTCAAAGCTCCAATGAAGGTACTTGTTTTCTCGCTGCTCATGCTACTAAGTTTTGTCTTTGT

IWB57879-2A ACAACAACAAAACAGCCAGCATCATTCTCATCATCTGGCACAAAAGCACAACACACACAGACACACTCAGGGGACTATAGGAC[G/A]CCGAGCGACCAACGAATCAATCAGCAACGCTTCTCGCCTGCTGCTCCGGGGGCGTTTCTTGACAGCGGCCGAGAGGCATGCAGGGGGA

IWB24221-2A GCAGGAAGCTGTATTTCATGATGGGGATAGGTTCCTCCTTCGATGGTGTCTTCACATGGAACAGGCCGTTCTCGAGGCTGCTCAACACGGTACTCTGCAAGGC[A/G]GCCCTCTTCATTCCAGAAGTACCCTCAAGCCGGAATGAGAACTCTGCCTCCCTCCCTGCTGCCTTCTTTGGTGGTAAGGTCT

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Table 6. Target sequences for the P91193D1-10 source of glume resistance on chromosome 2D. Target SNP shown in parenthesis.


IWB26954-2D GTGATAATGGCATTTTCATCTCATGTTCCTTACAAGACGAACTCATAATCGTACATGAACCAAAATTCAAACACACCGAACTATAAGCCACTC[A/G]CAAAACAAATAACCACATCAAACATAAAACTATATCACTCAGGTTATGTAGCACGGGTGTGCTAACTTAACCCAGTTCGTCCTGTGAG

IWB73791-2D TGGTTTGACTTGGCATGTCAATTTCTTAGAACCTATTGGTGTCAAGCATATATGTCACCTTACTTACAGGATCGGCAAATCACATGGTCT[A/C]ATTTTACCAAGGACCTTTAAAAAACTTCACCACATTCAGACACTTGATTTTGGTAATTGCAAACGTCTTTCGCTCTCCAAAG

IWB73799-2D ATAGATCCCCATGATGCTTACTTTTCCTAGTGTGTATTCTGGAAAATTTCGCTATGTAGGTTCCATAAGTACGAGCCATTACTAGTTCTG[A/C]TTATGCATCTTAAATTCCTTGGCTTAACCTGCCAAAAAACCAGCTGTACAGTTTGATCACAATGTTTCCAGCATCTGAACACA

IWB45312-2D

TGCACTGCAGGAAGGTTTGTTTGACACTGATCGTGACACGGGATCTGGTCTTGCCCTATCTTGTTCATTAGCTTGCAGAGAAGTAAAGAGTTCAAGTAAGAAAGTCCTGGCTGTATCCTCTC[A/G]CTAGTTGATGATGAGTTGATGCTAAAAAGGGACTCCAGGCAATCCTTAGCGACTTCAAGAGCTTCAGGGTCAGCCCCAGGAGCTAACTCAACTGCATTCCATATATGCTTCCAATGTCA

IWB21124-2D TTGAAGATCGTGTAGCTCCTAACAAGGATGAGACGCTTGGCCGCATAATTATACCATTGACGATGATTGATAGGCGGGCTGATGACC[G/A]TATTGTCCATGGGAAGTGGTTTAATCTTGAGAAGCCTGTACTTGTTGATGTGGACCAACTAAAGAGGGAGAAGTTCTCTAGCCG

IWB56596-2D TTTGTTGGCTTTACCAACTGGAAGCTGTGGCTTCTTCAAGTCCTCCTCAGTCAAGAGGCTGTCTTCGTCAATCAGTTC[A/G]TCATCATCGTCAATTTCAATCTTTGGAAGAGCCTTTTACTGCTTTCTTCAGGGGAAAGGAAGAACCCATAATCCAAGAAGGCTTCTTTGCCTTGATCT

IWB53436-2D GCGCTACACTTTGAGCAGACCTGTATAACCAGAGGACAGTATACAACACCAGCCAACCAACCAACCCAGGCAGGCAGAATCAATCAACCAATC[A/G]CATCATCATATAGGAAATCACACAATGTGCTACACCCGGGACACCTGATTGCGCAATCTCATGCCCTTCCCTCCTTTTCTCATGTTCAAC

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Table 7. Target sequences for GWAS on chromosome 1A. Target SNP shown in parenthesis.

SNP name QTL Target sequence

IWB8696 1A-1 CTCCTTTTACTCTTTGGCTGTCCAACAAACAGGGAATAAGAGTTGTGGCGAATCTGAATGGTGTTGATTTGGTGTTGACTTTTTACATACGTAACGAACA[A/G]TATGTATATTGTGTTCTTTTGAACTTTCTTGCTTCGAATTCTTARATCAATTTGTTTGATGTAGATGAACTTTTTATTACGTAAAATAAAATAAAACAAT

IWA8622 1A-1 GCTATCTGTCTGTGCGAAATACGGAGCTAGGGTACTGATAGATACCTCCTCCTCTGGTCTGGAGTTCCAAGCCACTGGCTGTAGCCAATGGAATTTGGAA[A/C]AATGTCTTTCTAATGTGAAGTCTTCAAAGCCCTCGTTCTCTGTTGTCCTGCTCGGAGAGCTGTCCTTTGAGCTGACCACGGCCGGGCTTGACTTTGGGTT

IWB48747 1A-1 TGAACTGCCATGCCAGTAAAGGTACTAATAAAGTGAATTGTTTGAAACAAATTAATTACCAGAAGAAAG

TTGTTTTTCCCTCATCTCAGATGTTAAAATC[A/G]CAGTTGCAAGGTCTTGATCAATGTCTGCAACCT

GCATGAAATTACTGAATAATATTCACAAGATAATGAAACAGCTCACTATAAGATCAGGTCCTCAGCA

IWB34823 1A-2 CGCTACTGAACCCTGGCCGTGCCCGGCTGTGTGGAGTAGCACCAGTGATATAAGCATTATCATTTGTCC

AACAGGATAAGATATTTCTTTCATACATTGG[A/G]TCCGAGCCTATGTGAGCCAGCGCGTTATTAGCA

CAGACCAACTTCATTCAGTTCAAGTACTCGGCATGCACCAAACTCTGGTGAGACTAGTAGGGATAGT

IWB11516 1A-2 TTATCTATTAGCTGGAAGGGATGCAGTAGGCTTTCCGCAGCTGGCGTGGCCCTGTTGTTCTTCAGCACGATGAAGCTGCTGGTCGAGGTTCATGCTCTGA[A/G]CTTGTATTGGCGTCGCCAGACGTTTTTGACGGATCCTGTATTTCTCATGATTTTACTATAAAGAAGATTTCGTCGACCTGAGTTAGAACCTTCTTATCTT

IWA4126 1A-2 CAGWTGGACGTGAATCAACCTTGGTAAAAGAGGCTCAGTCCTTTGGTTCTCTTTGTTATCAGATACCTA

AGCTAGAAGATATCGACCTTGTTCGTTCCCC[A/G]GTTTTGTCAAGACATCATGGATCAGAACACGGT

GTGGATGGATTTCAATCACCCACTGGCTATGCTACTCCCTCTCCAACTGATGGAAAACAATCCGATC

IWB10679 1A-2 TCAGTGATCTTGTACTCTGAAAATAGAACATTTACACTGAAGCTTCAAGAAAAATTTCAGTGATCTTGTACTCAATTTAGTGGCAATCAGACTTGAGATG[A/C]AAAACTGAAGCTTCGAAACTGAAACAATGACAAAATTACTTGGGAGTACTCCATTTATCTACCTCTGTCATGAAAGGAGTACTAGACAGCAGCAGTTGTA

IWB19625 1A-2 GTTCACAAATCGCAACAGTCATATATTATCAGAAGGAGATGTATTTCTCAAATAATAAAACAACCCAAT

AGGAGAATCCTACAGAACTTAAATGCAACC[A/G]TCATTATACCCCAAAATTACAAATCACTGTTTTC

TGCAGCGATAACAATCTGAAGAGGCACCATTTCTGTATATTCAAAAACAATTTTACAGAAATGGGG

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Table 8. Target sequences for GWAS on chromosome 1B. Target SNP shown in parenthesis.


IWB74534 1B-1 CAATTAAACGGTTGCAGTTTGATAAGCAACTGTCAATGGAAAATGTGAGGAAGCTCATAACAGAAGCTGATGGATACCAGCCTCACTTGATAGCTCCAGA[A/G]CAAGGATATCGGCGCCTCATAGAATCTTGTCTTGTTAGTATCAGAGGTCCTGCTGAGGCAGCTGTTGACACGGTATGCCGGCTAGATTAATCTGCTTAAT

IWA5352 1B-1 AGAAAATTTACCATGTCTTTGACAATCAGTTTCCTGTGGCAATTAAACGGTTGCAGTTTGATAAGCAAC

TGTCAATGGAAAATGTGAGGAAGCTCATAAC[A/G]GAAGCTGATGGATACCAGCCTCACTTGATAGCT

CCAGAGCAAGGATATCGGCGCCTCATAGAATCTTGTCTTGTTAGTATCAGAGGTCCTGCCGAGGCAG

IWB7144 1B-1 AAAGAATCAGCTATATATGCCCCCCAAGAACAGGGAAGAGAAGAGATCGATGTCACCAAACAAGGAGTA

AAGGTGAACACACACTAGGGCACGATAAGTC[A/G]ACAAAAAATTATTTTGACCATGCAACCGCGTCG

ATCTCTGCTTGGGCGCTCCGGTACAGCTCCAGCCTCTTGGCGAGGTTTGTCCTCCGCTCCATGACCG

IWA7422 1B-1 GCCAGCCGGAAGGATGATAAACCTTGCGGCGGCGCAGAGGTTCTGGATCTCGACTTGGCGATGCCCGCT

AGCGGCGCTGCTAACCGATTGCCCTCCTCCT[A/G]AACAGCCCGCCCGCGCGACGGGACCCGCAAAGT

CGTAGTGCCAGTCATACTAGTAGGAGTGCCAATCAGAACTGCCTGACCAGCTTTTTCTCTATTTTTG

IWB36125 1B-1

AGAATCAACTTCTACTGGACCGACAGGATTGTATGCAACAGCTGGTACCAACTCATTTTGCAAGTCTGA

ATCATTATCCACAATTATTGACTTTGAGCTAA[C/G]GTTTTCAAGCTTCGCCGTTGCATTGTCCTCAT

TTTCAACAGCAGATACAGCATGTGCTTCTGTTTGATCGTTCTCTTCCCCATTCTGGGCTGTATTAGTTT

CGCCATCAT

IWB29151 1B-1 GCCGAGCTTCATAGTAGATCAGGAGTCTCCTGGTCAGCAAGGAGAGGTCCACCGGGACGAAGCTATGGCACAGCTGGTGCACTCCCGGATGCTCAAGGAC[A/G]TGACGACCAGTGACTACGGCACCTACGACCCAACCCCGTCAATGCAGAAGCCCCACTTCAAGCTCATACCCAACTGAACACACCCTGGCTATACGTGATA

IWB36563 1B-1 TCACCTCGTCTGACGCTGGTTCACGCCTTCCATGCGTTATTGAGGGAGGGAGAGGAGAGCGCATGCATCGCGTGAGTAACCCGGAGATGGGGATGCCCCG[A/G]AGATCCGCCTCCCTCTTCGCCCTCGCCTTCTTCGCCCTGCTGCTCTCCACCTCCCTGGCAGGTTCGATCGTGTGCCTGTCTTGTCCTTCGTTCTAGCTAG

IWB46686 1B-1 GACCTTTCGGAGTCTGCATCAGGGTCCAGGGGGCACCCGGGTAGCGGTAACATCGGCTCGCCTCTGAGT

TATTGGTCTCTGAATAGGGTGGAGAGCAGCA[A/G]GTCTTGCACAAATGCCGCTCTCACGCTGGCTTG

CTCCCTTTCTTCCCTTGTGAGCGACGAGTCCATACTGCCAAGACAAAATGAAGCGGGTGACTGACTT

of 34



IWB47006 5A-1 TGTGATGCAGAGTACACCGATTACACTCCTTGCGAGGAGCAAAAGCGTGCAATGACCTTCCCTAGGGAT

CACATGATATATCGGGAGAGGCATTGCCCAC[A/C]TGAAAACGAGAAGCTGTACTGTCTGATTCCAGC

ACCAAAGGGTTATGTTGCTCCTTTTCCGTGGCCAAAGAGCCGCGATTATGTTTCTTATGCCAATGTG

IWA4744 5A-1 TTCCTATTAACCCTCGCTCATAGATTACACCTAAAGTAGAAGTATCTGCAATGAACGGAACGACATTCA

TGACCCACAGCTTTGGAGACTCCAGTACAGC[A/G]GCAAAGCTACCAAGACCTGCATTCATGTCCATT

ATGTTGCGGTACCTTCCAGTATCAAGCAAGTCATTGGTTTTCTTGTAAGCATTAACATGTTTCTTGC

IWB7241 5A-1 AGTTCTACGGTTCCACTTATATGGAAAGAGACAAGATTCATCTTCATTGCTTGTCACGGATTTCGATTCTTCACATAAAACTTTGACCAAAGAGCTCATC[A/G]ATGATGTGTGCATGTGCTAACAAAAGGTGAACAAAGCAACTTGAATGGGCGGAGACATACCCGGCACCGCAGAGTAACCACTCAAGAGGCGCACCAGGGT

IWB10765 5A-1 TCCGTTAGATTGAGGGGCCATTGATAAAAAGAAACGCCAAACATCTTACCAACTAATGTTACGAGCAGT

GCTTGCAAACATGATTTGATTCTTGCTAATC[A/G]ATATACTACCCCTTTCGTGCAAAATATAAAATG

TAGCGAGGTAGGTTGATGTGTTCAACATCGCCGATCTACTGGCATAGGAGCTTGGTACACGCGTAGG

IWB686 5A-1 GATGCCTACCCTCGTGTTAGGGCGTGGCTGGAGGAGCTGTTCGATAGGCCCGCCGTGAAGAAGGTCATC

TCGCTCATGGCCAAGGACTTCAATTAGTTAT[A/G]CTTCTGAGTAAAGGTCGCGCCTGTCAGGTTACA

TTATGTGGGAGACCGTATGCAATAAGACCAAGGTCGACTGCATGTATTGTCTTTACATCTCACCACA

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IWB26333 5B-1 TAGGGTGCGGACCTTGTGCATCACGATTGGGAGTACCAGCAGAACCAACACTATCAAAAAACATTTGCTGGAAATTGGGATCCATTAGTGCAAAAGGGTC[A/G]TGCAGAGCTTCAGGGAACAATGGTCTTTGAAACGTGTTATCAAGTGGTCCGTCCAGCTCCATATCATCATGATTAACAGGTGGAATACTTTGATTGATTG

IWB72334 5B-1 CAAAATCGTCCGACTCTGAAAATGTAACCCCGCTGTACATACATGGTCCATCCATCACGGGATATCCTG

CCCATGATCAAACAGATGATCCCTTCTAATC[A/G]CTTCTCCGGCAATAACAAGCAGTTTTGTTTCGT

CACTGGACCTGAAGAAAATACTTGTGTTAGTACCTAAAGTACAGTTCGTTCGGCAACCAAAAAGATC

IWB9615 5B-1 CTGCTTGCCGTCTTATTATCTTATATACGACCAATGTACAATAACTTAAAACTACTGTTGCATAAATCA

CAAGTCACAAGCATCCGTATTTACCTTGTTC[A/G]CACAAACATTAGGAGGATGAAATGAGACTTAAT

GATGTTCGGCAAGAGAGGATCTTCCTGAGGAACTCACACAGTCTGGCAAATAATGCAGCATTTGTGC

IWB57255 5B-1 CTTCTTTCGGTTTTCGACGAAAACATGTAGTGTTGGGAGGACCATTTTTCATGCTTGTATGTTTGTAGATAGGAACTGTGGTCTGTTAATTAAGTTTGTT[A/C]GTTAGTGAATTCTGTAAATTTGTTTGCCAGGCCCTGTAAGTTTGTTACTCGAGCACTCCAGTGATTTCATGCGTGTTTTTGTGTAAGAGGCCGCATCGTT

IWB66159 5B-1 TAGCCATCATTACAGTGGCTTTAAGCAACATGGAACTGAATGCATAAACCCTTGAGGTGTCGACACTAA

CCATGCTCCCCACAAACATTTTGTAAGAGTA[A/G]AATGATAAATAACTTAAGATGGAAATAGATGTA

CAGCCAAGAGCTACAGACTATAAGCACGCAGTGAAACATATATTCAACAAATTTGGAATTAAATGTA

of 34



IWA6999 6A GGCAACGCGATAACATACGTAAGTGTTGCCAACGAAGCACGGGGCATCGCCTCAGGTGACTTGGCCTACATAGTGCGCCGGATTAGAGACAGCCTCAGCC[A/G]GGTGGACGATGAGCTAGTGCATTCTGCARTCGACTACCTCGAGCTAGCAAAGACGGACAACCGCCCTGCTACAGGCAGTTTGCCAGCGACAGATATGAGA

IWB44292 6A AGGCACAAAAGCTGGCTGATTTTGCGTTGGACCGGGACTTTGAAGTTAGCTTCTCAACCTATGACAGAG

TCCTGGAAGCCCTGTACACTGAGGAGAAGAC[A/G]TTGCCAGCCTACTCCATGCTCTGCAAGATCAAG

CACAAAGGAGGCATTGTAGACCAGAAGGGTTGCGATGCTTTGATGGATAGCTTAAAAGCTGGAGGAT

IWA4961 6A AGGATGAATTGAGGAACGGAGGAAGTGGAGATCTGGAGGATGACCTTGCTGGAAAGGACAGATACATGGGTTCAATCGAAAGTCTCATCCGCAATAATAT[A/C]CAACAATACAAAATTGATATGGCAGATGAAGTCATTAATGCTGGTCGTTTTGATCAAAGAACGACCCATGAGGAAAGGCGCATGACTCTGGAGACWCTGC

IWB29622 6A TGTGTTGGCCCTCGGTGTAGTGACTCCTAGGTCCTCCGGGAGCACCTGCCTTGCTACCACCGCCGCTAC

TCTCTTCACCTATGTTTCCTCGGCTGCCGGCT[A/C]TGTGGCATCATTGTGGGTCACTTGTAGGGCTC

TCATCCCTCGTCCTTAGTTCGACGTGGTTTTCTTGGATCTGTCTCGGGTGTTGTGTCTTTAAACTATC

IWB8366 6A CAGGGTATTAAATTACATGTCCACGGCAAACCAAGAACCAACATATGCTGGTTCTAATACAGTACAAGA

AACAGCATACCAGTCTCGGACGATGGCATGC[A/G]CCTTGAATGCATACATATATCCTGACTAGTTCT

AGCACAATACACTTGAACATCGATTGATACATGATAAATCTCCAGAGTATTTATCAAATTACATGAG

IWB1211 6A TTTGTAGGGGATACATGGCACCCGAATTCTTCATAGGACAAGTCACATTTGCATCAGACATATACAGTCTTGGTATTATAATTATGGAGATTCTGACAGG[A/G]GAGAAGGGGTATCAGGAAGAACAGGATGTAAGCATAGTTTTAAGCATGTGCTCCATGTTAAGGATATATTTTCAAGTAAAGTACGCCAACGGGATAATTT

IWA1283 6A CCAACTGGCGCTGGTTCATCCTCTCGACRCGGAGCTTGCCATAGGCCTTGAACTCCTTCATCTCCTCTGTGACCTTCACGACCTCAACTGAGCGCTTCTC[A/G]CCGCGGGCAATGGGCATGTAGTCGCCCTGGACCTGTGTGGCGTTGGCAAGCTCCKCCGGAGTAGAGTCACCAGCCTTGACCTTGCGAGCACGCCTTGGGA

of 34



IWB35428 7A TTGTAGTTGACGGAAATTCCATTAGAAAGGCGACGTTGTGTTATACCNGTTTCATTGTCAAATACTTTC

[A/G]CCACATCATCCTCTTTGAAAGGAACAAATGATGGTCGGTGTTGCACTTTCAAGTCTTCAAGTTC

TGATTGAGTAATCAACTCTTTTGGCACCTCAAGCTC

IWB58340 7A ACTATGTGAGAAGCACCTAAAGGGGCAGCCGTGACATTCGTTTACAGGACTATAAAGATTAGAATAAGC

ATACCAGTTGCCATAAGAGGTTTTGCATTTG[A/C]GTTTGCCCAGCTTTAGTAGTCCTTAACCAATAC

CACCACTTTGATGGTTGCAGAACTTGAATATATCTGACCCTCTCATTATCATCACCAGTAACTAACT

IWB58339 7A CCAGAACAGAACATCTCAGCTGTTGAAGAATCACTGAAGCATGTGGCTAGGTTTATAGAATCTTTTGAA

TCAGAATCAAGGGAAATATGGCTTGTGTACC[A/G]CAATGAAGGCCGCTCATTGTCAAAATTGTTATA

TGCTGCTGAAGAAACAAAGTTAGTTACTGGTGATGATAATGAGAGGGTCAGATATATTCAAGTTCTG

IWB72890 7A AAGCAGTTGTATTCTGACACCAGCTACAACACAACAGCACCTGGACTGCAGCGCCACGGCGGGCAACAA

TCGGTGTGAAGAAAGAATCCTTGAGGCATCC[A/G]TCATACTCATATGACGGTGAAATTCGAGAAAAT

GTTCATCAGAGTGTCGAGCGCCTCGGGATAAAATTTCCGTGCGAATAGGTAGCAGGGCCTCTTCATG

IWB3398 7A ATTAAAGCTTGATACCAGCTTTCATAGATCCGGATGGTTTTGAAGTGATAGCTGGCTTGACTTTGCTTTTCTTCAAAGCATCTTG[A/G]CAGACCAAAATCCGTAATTTTTGGCACCATGTTTTCGTCTAGCAATATATTTGCAGGTTTTAGGTCTAAGTGAAGGATATCGATCTCTGTTAATATATTG

IWA2011 7A GTCAAACTGATACGGGGCGCTTAGTTTGTAGTGGGGTCAAACAACCCATTCATTTGCAGTGTATTCAGC

CCAAGTAGTTCGAAATTTTTGGGTTGAAAGT[A/G]TGAAGTACAGATGTTAGAAAACGTAGTTGAGTG

GTTGTAAGATTATATATATACTGCCGCTAGGCTTGTATGAGTATGACGATCATGTTCTGATGTAGAA

of 34



IWB73291 7B TGCAGCTCTCTGGTGCGGGTGTTGCCGCGGTGGCCTTCAGCAACAATGGCCTGCGCCTCTCTCAGACGT

CGGCACTAAGGGTGTGCTCTTCCAGGCGCTC[A/G]TCTCGTAGCCTCGTTGTCAAGGCTGCCACTGTC

GTCACCCCGAAGGTTAACAACTGTGGCAGTGCAAATGCATTATGCTTAGGGGGTTCTTATTTACGCG

IWB7527 7B GCCTACATATGCTACTGCTGTTGTACAGAGATAATTCATGTGCAGATGGATGGCCTACGTTAACTACAGAAGTCAATTTGTCAACTACAGATGTCAGTCA[A/G]TGACAACCCCCTACTACCCCTCCCTCTTGTTTCATCATCCACCAGATGTAGCTACTTGTTCTCTGTTACAAGACTAAACTAATGACTGCAGAGATGACAA

IWA809 7B TCTGGGGCAAGATCACCCGCCCGCACGGCAACTCCGGCGTCGTCCGCGCCCAGTTCAAGTCCAACCTCCCCGCCGAGTCCATGGGGCGCAAGGTCAGGGT[A/G]TTCATGTACCCGAGCAGCATCTAAGGTTTTTTCGATCTAGGGACAGTAATGCTTGTTTCTTGTGATTGTCATGAGCTTTTTATGGTGAATCTATGCTATG

IWB10705 7B AAAACAAATCAAAGAACACAGAATCCTACATCAACCATATTGACCCAAGCCAAACCATTAGTATCTCAA

TGGACACATTGCACAAGTTCATATACTTCAG[A/G]CATACTATATATTTCGACCTCACTTGGAATTCT

ATAAAATCCATCCTAGTCATTCACAAGGCCTAGGACAAAATGTAAGACACATGCTAGAGTAAATCAT

IWB73677 7B ATTCTAGGGTTGCGTGGTTCAGCATCTTGTCTCTGGGCGTGTGCGTTGTGGTCTCCGCTCTGCAGTTAT

GGCATTTGCAAGGCTTCTTCCAGAAGAAGAA[A/G]CTCATCTGAATTCACTCGTTTCACCATGTACTC

CTGTGTTGTTAACTACCTTAGCAAGAGGACAGTTTTGTCGTATTTATTTCGACAAACTCATGTGGTG

IWB7285 7B TGCGTGGTTCAGCATCTTGTCTCTGGGCGTGTGCGTTGTGGTCTCCGCTCTGCAGTTATGGCATTTGCA

AGGCTTCTTCCAGAAGAAGAAGCTCATCTGA[A/G]TTCACTCGTTTCACCATGTACTCCTGTGTTGTT

AACTACCTTAGCAAGAGGACAGTTTTGTCGTATTTATTTCGACAAACTCATGTGGTGGACAAAAAGG

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Table 14. Summary of SNP allele calls. Highlight indicates allele observed in resistant parent.

Population SNB resistance

SNP name SNP allele 1

SNP allele 2

EGA Blanco/Millewa

Flag Leaf

IWB11788-1B A G

IWB45437-1B A C

IWB23711-1B G A

IWB55607-1B A G

IWB63360-1B A C

IWB26464-1B A G

IWB29507-1B G A

Flag Leaf IWB12583-5B G A IWB22904-5B A G

IWA4103-5B A C

IWA1780-5B A G

IWB39412-5B G A

IWB73666-5B A C

IWA7910-5B A G

IWB27379-5B G A

6HRWSN125/WAHT2074

Flag Leaf IWB35441-2D A C IWB66107-2D C A

IWB41295-2D A G

IWA8151-2D T C

IWB28099-2D A G

IWB46396-2D A G

IWB7616-2D G A

IWB38687-2D G A

Glume IWB56078-4B A G IWB73258-4B A G

IWB72955-4B G A

IWB4720-4B A C

IWA1846-4B A G

IWB71675-4B G A

IWA3325-4B A G

IWA4055-4B A C

IWB28921-4B A G

P92201D5-2/P91193D1-10-2-92

Flag Leaf IWB41604-2A A G IWB29103-2A A G

IWA3122-2A A G

IWB9206-2A G A

IWB57879-2A G A

IWB24221-2A A G

Glume IWB26954-2D A G IWB73791-2D A C

IWB73799-2D A C

IWB45312-2D A G

IWB21124-2D G A

IWB56596-2D A G

IWB53436-2D A G

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Table 15. Summary of SNP allele calls in population Scout/ZJN12 Qno25//149ZIZ:13. Both ZJN12 Qno25 and 149ZIZ:13 have known sources of resistance to SNB. However the allele effect estimate indicates that the 149ZIZ:13 parent is the likely source of resistance. Highlight indicates allele identified for use in selection.

Parent IWB74534 IWB7144 IWA7422 IWB36125 IWB29151 IWB46686

Scout A A A C A A

ZJN12 Qno25 A A A C A A

149:ZIZ13 G G G G G G

Supporting your success

Table 16. Fluidigm primer sequences.

SNP name ASP1_SEQ ASP2_SEQ LSP_SEQ STA_SEQ

IWB11788-1B CAACCGTCTAGCGACACGT ACCGTCTAGCGACACGC GCGCCGTTCACCTGCA TCCGTCAACAGAAGTCCCTT

IWB45437-1B AGGTATTGATCCTTGATCGTCTTCAA AGGTATTGATCCTTGATCGTCTTCAC GCAAGGGACCTGATGAACCC TTGATGACTGAGATTCAGAGTCAGA

IWB23711-1B AGATCATCCTTCTGAAGGAAGCG AAGATCATCCTTCTGAAGGAAGCA GACGTTGGCAGTAGTAGCCCT CGCTTCATCGCACGAACAA

IWB55607-1B GTTGAGCCTGAGAGACACCAA GTTGAGCCTGAGAGACACCAG GATGACTCTGGGCTTGCTTGTT TGACGTTATTTTTGGTGCGGA

IWB63360-1B AATTTGCAAATGAAGTCATCATCCAATCTA TTGCAAATGAAGTCATCATCCAATCTC GGCAACAACCAATGAGCCTGA GCCGATTAATGGTAGTGTGCT

IWB26464-1B CTTTGTTGACGAAATCGATGTTCTGT TTGTTGACGAAATCGATGTTCTGC TGGTCTTCGAACCCAAGTTGGA TGCTGAGATCACTGGCGAT

IWB29507-1B CCCCAAAGCCTTCTGCG ACCCCAAAGCCTTCTGCA ACATAAGGGCGCTCACTGGA CATAAAGCCTTCCATACAAGCAAC

IWB12583-5B GCCATCTGGTATCAACATCTGTTG GGCCATCTGGTATCAACATCTGTTA GCTTTGCTTTGCCGCACC AACATTTCAAAGTGTCAGGCCA

IWB22904-5B GCTGATCATCCAACAGGACACTA GCTGATCATCCAACAGGACACTG TTGATGGAGGCCTCAGTGACA GACAGAGTCCAAAACAGAGTAACC

IWA4103-5B CAACCGCAGGGCGAGA ACCGCAGGGCGAGC CTACGGTCGAAGGCGGGA TCATCAATGGTTTTCGCTCTCATG

IWA1780-5B CAAATGGACCTGAGTTCTTTTACGAA CAAATGGACCTGAGTTCTTTTACGAG AGCACGCATCCTCCTTGCT CCAAGTTTGGTAGTGGACAAGC

IWB39412-5B TGGACACTCATCAAGCTGCG TGGACACTCATCAAGCTGCA GTTGCTGGAGAAATGCGCGA ATGGGTCCGTGTAGCGT

IWB73666-5B TCTCATACAACAGTTGGAACGGA CTCATACAACAGTTGGAACGGC GTGATGGTTAGCGTGCATTTTCGT GGGTCTCCACTATTTTGGTCTC

IWA7910-5B AGATGACTAAAACCCGTCTTGCA GATGACTAAAACCCGTCTTGCG CGTCGTCATTTTTGTAATCTATCAACCTAGC ACTGCACTTCTGTGTGCAAC

IWB27379-5B CTGCCCAATGTAGACAATTCACTTG ACTGCCCAATGTAGACAATTCACTTA GTGGCGACATGCATATCACCAT CTGTTGGATCTTAGGACTGCC

IWB35441-2D GGATTCACTAGAAGAAAGAGGTGCA GGATTCACTAGAAGAAAGAGGTGCC AGGCTGTGCCGGACTATGT CATGAACAGGACTCGCCG

IWB66107-2D GTGGTTATTGTTGGTCGGAGC TGTGGTTATTGTTGGTCGGAGA CAGGAAGCGCCTGTTTGGT TGGACTTGGATTTGCTATTGCTG

IWB41295-2D TCCTTCATCTCAATTATCTGCCTATGAAT TCCTTCATCTCAATTATCTGCCTATGAAC CCAAGTGAGGATGATCCGGTCA GCATTCGTTTCTTTCCATTTTAAGCA

IWA8151-2D CCTTATCCAGTGCAAGTCGATCATA CTTATCCAGTGCAAGTCGATCATG GCAGAAGTTCACCACTGCCT CCTAGATCGTCTAGAAGCATGCC

IWB28099-2D TTGAAATGTTCGACAAATGTGCCA TGAAATGTTCGACAAATGTGCCG CGCCTCATCAGGAGTACTGCA ACACCAGGAATCGAGTCTTCA

IWB46396-2D TGGCCTCAAAATGTCTACTAAGAAACT GGCCTCAAAATGTCTACTAAGAAACC GTCCTGACTGGGGTCGGA ACCATGCAGGCCCCTC

IWB7616-2D ACAAGGAGCACCAGCACG ACAAGGAGCACCAGCACA GCTCGGTGCTTGAGTTGGC CCACGTTGATCGCCCTC

IWB38687-2D GAAACCACATGCCGCTGG TGAAACCACATGCCGCTGA TGCACAAGCCATGTGGCAA CTGAAACCACATGCCGCT

IWB56078-4B GTGTCTTTTATAGTGTCCAAAAAAGTGTGT TGTCTTTTATAGTGTCCAAAAAAGTGTGC ACCGTCTCCAACCAGCCTT ACGTGGAGGTGGTGTCTT

IWB73258-4B GGAACAATCACTCTGAGCAATAAACA GGAACAATCACTCTGAGCAATAAACG ACCTGGCTTTGTTTTGTTCCTTTTTGT AGACTACTTTGGGAACAATCACTCT

IWB72955-4B ACATGTGGCACACATTGTTGG TTTACATGTGGCACACATTGTTGA TCCATGAGGAAAAGGCGGTCT GCTTCTATTCTTTGAACATGGACTGT

IWB4720-4B CCAATCCGCGACTCGACTA CCAATCCGCGACTCGACTC ACCAGCTTCGGATCCATCGG CCTCTCAGCCTCGGCTC

IWA1846-4B CGACAAGGAACTGGAACAGTACATT CGACAAGGAACTGGAACAGTACATC GGCCATCCTTGACATCCCAC AAGCAGATGTTTAAGTTCAGCTCC

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IWB71675-4B GGGATTTCTCGGCCCGATC GGGATTTCTCGGCCCGATT AGGGGTCAGCAGCCCG GCGGCCCATTTGGTGTC

IWA3325-4B GATAGGACCACGATGTCTTCACA ATAGGACCACGATGTCTTCACG CCTCGTACATTTGAAAGTCTAGAGCAGA CCTCTGCATCATTTAGTTCCTTGG

IWA4055-4B ATGGTTCTTCCCCAACCCAA TGGTTCTTCCCCAACCCAC GCCCTTGTCCAGCCCC CATCAAACAGATGCACTGCG

IWB28921-4B CGTTGTGCGCCGTCGA GTTGTGCGCCGTCGG CGTAAATGGCAGGACGCACC CTGTCGTCGCATGACGC

IWB41604-2A CGACCAGCACGGACTTTGTT GACCAGCACGGACTTTGTC CCCAGGCACACCAAGGTT TCTTGACCACACCCTGCA

IWB29103-2A AGCTATGCAGCAGCAGCTA GCTATGCAGCAGCAGCTG AGCCATCCCATAGGGCCAG TGGCTATAAGATGAACTTTGGCAAT

IWA3122-2A TCGACCTGGTAGCCTACAGT CGACCTGGTAGCCTACAGC AGGGAGAGCCCTGCGAAC TCTACTCGATGGGCGGTG

IWB9206-2A ACAGAAAATTGCGATGCCAGAC AAACAGAAAATTGCGATGCCAGAT GGCTACGTTGCTATACCAAGCAC TTCATTGGAGCTTTGAGGTAATCTC

IWB57879-2A TCGTTGGTCGCTCGGC ATTCGTTGGTCGCTCGGT TGGCACAAAAGCACAACACACA CGAGAAGCGTTGCTGATTGATT

IWB24221-2A GTACTTCTGGAATGAAGAGGGCT ACTTCTGGAATGAAGAGGGCC CCGTTCTCGAGGCTGCTCA TCTCATTCCGGCTTGAGGG

IWB26954-2D CACACCGAACTATAAGCCACTCA ACACCGAACTATAAGCCACTCG GCACACCCGTGCTACATAACC CGTACATGAACCAAAATTCAAACACA

IWB73791-2D GGATCGGCAAATCACATGGTCTA GATCGGCAAATCACATGGTCTC TGGTGAAGTTTTTTAAAGGTCCTTGGTAAAAT GGTGTCAAGCATATATGTCACCTTA

IWB73799-2D CATAAGTACGAGCCATTACTAGTTCTGA AAGTACGAGCCATTACTAGTTCTGC GGCAGGTTAAGCCAAGGAATTTAAGA TGGAAAATTTCGCTATGTAGGTTCC

IWB45312-2D AGTCCTGGCTGTATCCTCTCA GTCCTGGCTGTATCCTCTCG AGGATTGCCTGGAGTCCCTTT GCTTGCAGAGAAGTAAAGAGTTCA

IWB21124-2D GATTAAACCACTTCCCATGGACAATAC AGATTAAACCACTTCCCATGGACAATAT GAGACGCTTGGCCGCAT ATCAACAAGTACAGGCTTCTCAAG

IWB56596-2D TTCCAAAGATTGAAATTGACGATGATGAT TCCAAAGATTGAAATTGACGATGATGAC CCTCCTCAGTCAAGAGGCTGT CCCTGAAGAAAGCAGTAAAAGGC

IWB53436-2D CAGGCAGAATCAATCAACCAATCA CAGGCAGAATCAATCAACCAATCG TGTCCCGGGTGTAGCACA AACCAACCAACCCAGGC

IWB8696 GTGTTGACTTTTTACATACGTAACGAACAA TGTTGACTTTTTACATACGTAACGAACAG TCGAAGCAAGAAAGTTCAAAAGAACACAA GGCGAATCTGAATGGTGTTGA

IWA8622 GCTGTAGCCAATGGAATTTGGAAA GCTGTAGCCAATGGAATTTGGAAC CGAGCAGGACAACAGAGAACG GGAGTTCCAAGCCACTGG

IWB48747 CATTGATCAAGACCTTGCAACTGT ATTGATCAAGACCTTGCAACTGC GAAAGTTGTTTTTCCCTCATCTCAGATGTT AGTAATTTCATGCAGGTTGCAGA

IWB34823 GGCTCACATAGGCTCGGAT GGCTCACATAGGCTCGGAC TCATTTGTCCAACAGGATAAGATATTTCTTTCATACA GTCTGTGCTAATAACGCGCT

IWB11516 GGTCGAGGTTCATGCTCTGAA GGTCGAGGTTCATGCTCTGAG CCGTCAAAAACGTCTGGCGA AGCACGATGAAGCTGCTG

IWA4126 CGACCTTGTTCGTTCCCCA CGACCTTGTTCGTTCCCCG AGCATAGCCAGTGGGTGATTGA AGATACCTAAGCTAGAAGATATCGACC

IWB10679 TTTAGTGGCAATCAGACTTGAGATGA GTGGCAATCAGACTTGAGATGC GTCATTGTTTCAGTTTCGAAGCTTCAGT GCTTCAAGAAAAATTTCAGTGATCTTGT

IWB19625 GAATCCTACAGAACTTAAATGCAACCA GAATCCTACAGAACTTAAATGCAACCG CGCTGCAGAAAACAGTGATTTGTAATTTTG ACAACCCAATAGGAGAATCCTACA

IWB74534 GAGGCGCCGATATCCTTGT AGGCGCCGATATCCTTGC ACCAGCCTCACTTGATAGCTCC CAGGACCTCTGATACTAACAAGACA

IWA5352 TGGAAAATGTGAGGAAGCTCATAACA GGAAAATGTGAGGAAGCTCATAACG GTGAGGCTGGTATCCATCAGCT TGCAGTTTGATAAGCAACTGTCAA

IWB7144 CACACTAGGGCACGATAAGTCA ACACTAGGGCACGATAAGTCG CGACGCGGTTGCATGGT CCAAACAAGGAGTAAAGGTGAACA

IWA7422 ACCGATTGCCCTCCTCCTA CCGATTGCCCTCCTCCTG GCACTACGACTTTGCGGGT CTAGCGGCGCTGCTAA

IWB36125 AACGGCGAAGCTTGAAAACG AACGGCGAAGCTTGAAAACC GCAAGTCTGAATCATTATCCACAATTATTGACT GCTGTTGAAAATGAGGACAATGC

IWB29151 CCGTAGTCACTGGTCGTCAT CCGTAGTCACTGGTCGTCAC TGCACTCCCGGATGCTCA GGGCTTCTGCATTGACGG

IWB36563 GATGGGGATGCCCCGA ATGGGGATGCCCCGG AGAGCAGCAGGGCGAAGA CGCGTGAGTAACCCGGAG

IWB46686 GCGGCATTTGTGCAAGACT GCGGCATTTGTGCAAGACC ATCGGCTCGCCTCTGAGTT GCAAGCCAGCGTGAGAG

IWB47006 AGACAGTACAGCTTCTCGTTTTCAT AGACAGTACAGCTTCTCGTTTTCAG CGGGAGAGGCATTGCCCA GGAGCAACATAACCCTTTGGTG

IWA4744 GCAGGTCTTGGTAGCTTTGCT CAGGTCTTGGTAGCTTTGCC TCATGACCCACAGCTTTGGAGA TACCGCAACATAATGGACATGAAT

IWB7241 CATAAAACTTTGACCAAAGAGCTCATCA ATAAAACTTTGACCAAAGAGCTCATCG CTCCGCCCATTCAAGTTGCT TGTCACGGATTTCGATTCTTCAC

IWB10765 TGCAAACATGATTTGATTCTTGCTAATCA GCAAACATGATTTGATTCTTGCTAATCG TCGCTACATTTTATATTTTGCACGAAAGGG CAACTAATGTTACGAGCAGTGCTT

IWB686 GCGCGACCTTTACTCAGAAGT CGCGACCTTTACTCAGAAGC ATCTCGCTCATGGCCAAGGA TCTCCCACATAATGTAACCTGACA

IWB26333 GGATCCATTAGTGCAAAAGGGTCA GATCCATTAGTGCAAAAGGGTCG CATTGTTCCCTGAAGCTCTGCA CAAAAAACATTTGCTGGAAATTGGG

IWB72334 TGCTTGTTATTGCCGGAGAAGT GCTTGTTATTGCCGGAGAAGC CACGGGATATCCTGCCCATGA GGTCCAGTGACGAAACAAAACT

of 34


IWB9615 CAAGCATCCGTATTTACCTTGTTCA CAAGCATCCGTATTTACCTTGTTCG CAGGAAGATCCTCTCTTGCCGA ACTGTTGCATAAATCACAAGTCACA

IWB57255 GGAACTGTGGTCTGTTAATTAAGTTTGTTA GGAACTGTGGTCTGTTAATTAAGTTTGTTC ACTTACAGGGCCTGGCAAACA GGACCATTTTTCATGCTTGTATGTT

IWB66159 CTCCCCACAAACATTTTGTAAGAGTAA CTCCCCACAAACATTTTGTAAGAGTAG GCTCTTGGCTGTACATCTATTTCCATCT GGTGTCGACACTAACCATGC

IWA6999 GATTAGAGACAGCCTCAGCCA GATTAGAGACAGCCTCAGCCG AGAATGCACTAGCTCATCGTCCA GCCTCAGGTGACTTGGC

IWB44292 CCTGTACACTGAGGAGAAGACA CCTGTACACTGAGGAGAAGACG GCTTGATCTTGCAGAGCATGGA TGACAGAGTCCTGGAAGCC

IWA4961 TCATCTGCCATATCAATTTTGTATTGTTGT TCATCTGCCATATCAATTTTGTATTGTTGG AGGATGACCTTGCTGGAAAGGA TCAAAACGACCAGCATTAATGACT

IWB29622 TGACCCACAATGATGCCACAT GACCCACAATGATGCCACAG CGCCGCTACTCTCTTCACCT GGGATGAGAGCCCTACAAGT

IWB8366 CTAGTCAGGATATATGTATGCATTCAAGGT GTCAGGATATATGTATGCATTCAAGGC ACCAGTCTCGGACGATGGC CGATGTTCAAGTGTATTGTGCTAGA

IWB1211 TGTTCTTCCTGATACCCCTTCTCT GTTCTTCCTGATACCCCTTCTCC CATATACAGTCTTGGTATTATAATTATGGAGATTCTGACA GGAGCACATGCTTAAAACTATGCTT

IWA1283 CCTCAACTGAGCGCTTCTCA CCTCAACTGAGCGCTTCTCG ACACAGGTCCAGGGCGA TCCTCTGTGACCTTCACGAC

IWB35428 TCCTTTCAAAGAGGATGATGTGGT CCTTTCAAAGAGGATGATGTGGC TCCATTAGAAAGGCGACGTTGTGTTA GCAACACCGACCATCATTTGTT

IWB58340 GGACTACTAAAGCTGGGCAAACT GACTACTAAAGCTGGGCAAACG AGCATACCAGTTGCCATAAGAGGTT CCATCAAAGTGGTGGTATTGGTTA

IWB58339 AGGGAAATATGGCTTGTGTACCA AGGGAAATATGGCTTGTGTACCG ACAATTTTGACAATGAGCGGCCTT GCATGTGGCTAGGTTTATAGAATCT

IWB72890 AGAAAGAATCCTTGAGGCATCCA AGAAAGAATCCTTGAGGCATCCG GGCGCTCGACACTCTGATGA GGGCAACAATCGGTGTGA

IWB3398 GCCAAAAATTACGGATTTTGGTCTGT CCAAAAATTACGGATTTTGGTCTGC GCTGGCTTGACTTTGCTTTTCTTCA TGCTAGACGAAAACATGGTGC

IWA2011 TCAACTACGTTTTCTAACATCTGTACTTCAT CAACTACGTTTTCTAACATCTGTACTTCAC GCCCAAGTAGTTCGAAATTTTTGGGT ACAAGCCTAGCGGCAGTATATAT

IWB73291 CTTGACAACGAGGCTACGAGAT TTGACAACGAGGCTACGAGAC TCAGACGTCGGCACTAAGGG GTGACGACAGTGGCAGC

IWB7527 CAATTTGTCAACTACAGATGTCAGTCAA CAATTTGTCAACTACAGATGTCAGTCAG AGCTACATCTGGTGGATGATGAAACA TGGCCTACGTTAACTACAGAAGTC

IWA809 TGCTGCTCGGGTACATGAAT TGCTGCTCGGGTACATGAAC CTCCCCGCCGAGTCCA CTGTCCCTAGATCGAAAAAACCTT

IWB10705 CACATTGCACAAGTTCATATACTTCAGA CACATTGCACAAGTTCATATACTTCAGG TGGATTTTATAGAATTCCAAGTGAGGTCGAA GCCAAACCATTAGTATCTCAATGGA

IWB73677 CAAGGCTTCTTCCAGAAGAAGAAA CAAGGCTTCTTCCAGAAGAAGAAG AACACAGGAGTACATGGTGAAACGA GCTCTGCAGTTATGGCATTTG

IWB7285 TTCCAGAAGAAGAAGCTCATCTGAA TTCCAGAAGAAGAAGCTCATCTGAG CAACACAGGAGTACATGGTGAAACG TGGCATTTGCAAGGCTTCT

SNP marker protocol for tracking SNB resistance in flag leaf and … · 2019. 2. 20. · Page 3 of...

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Transcript of SNP marker protocol for tracking SNB resistance in flag leaf and … · 2019. 2. 20. · Page 3 of...