SmartPull manual 1 02 - Warner Instruments...2018/08/10 · UniPix Smart Pull Operation manual Rev....

UniPixSmartPull OperationmanualRev.1.02 page1/16

Smart Pull Micropipette puller

OperationManualRev.1.02

DesignedinSwitzerland

AssembledinSwitzerland

UniPix,SwitzerlandWeb:www.unipix-lab.com Email:[email protected]


DISCLAIMERThepipettepullerSmartPullisdesignedforthespecificuseofcreatingmicropipettesandnootheruseisrecommended.This instrumentcreates itemsthatshouldonlybeused ina laboratoryenvironment. It isnotintendedforuse,norshouldbeused,inhumanexperimentation,orappliedtohumansinanyway.Thisisnotamedicaldevice.DonotopenorattempttorepairtheinstrumentwithoutexpressedandexplicitinstructionsfromUniPix.Hightemperatureispresentandcouldcauseinjury.Donotallowunauthorizedandoruntrainedoperativestousethisdevice.Anymisusewillbethesoleresponsibilityoftheuser/ownerandUniPixassumesnoimpliedorinferred liability fordirectorconsequentialdamages fromthis instrument if it isoperatedorusedinanywayotherthanforwhichitisdesigned.SAFETYWARNINGSANDPRECAUTIONS• Donotdisassemblethesystem.Referservicingtoqualifiedpersonnel.• Topreventfireorshockhazarddonotexposetheunittorainormoisture.OPERATIONAL• Makesuretooperatetheinstrumentonlywhenthecoverisclosed.• Toavoidburnsdonottouchtheheatingfilament,themetalpartsholdingthefilament,or

theheatedendsofglasspipettesthathavebeenpulled.• OnlyuseUniPixreplacementheatingfilaments.• Failuretocomplywithanyofthefollowingprecautionsmayresultininjurytotheusersof

thisdeviceaswellasthoseworkinginthegeneralareanearthedevice.• The micropipettes created using this instrument are very sharp and relatively fragile.

Contact with the pulled micropipette tips, therefore, should be avoided to preventaccidentallyimpalingyourself.

• Always dispose of micropipettes by placing them into a well-marked and spill-proof“sharps”container.

• Useonlywithcapillaryglass(tubing)recommendedbyUniPix


The micropipette puller you always have dreamed to have Basedonthecutting-edgetechnology, thisaffordableelectrodepullerwillbringyoumultipleadvantagesandsetnewqualitylevelinelectrophysiologymicropipettespreparation.Designedwithatouchscreenwhichhelpyouselectingthepullingsequenceofyourchoiceandmemorizingyourfavoriteparameters,specialattentionwasplacedintheelaborationoftheuserinterfacetosimplifyyourday-to-daylifewhilekeepingthehigheststandard inmicropipettesfabrication. Using a long-standing experience in electrode pulling our engineers have placedspecial attention in the design of the electrode handling, the mechanical drive, force andtemperaturecontroloftheheatingfilament.Programmingofanelectrodepullerhasneverbeensosimpleandasingletouchwillbringyoutothenextlevelofsophistication.Builtaroundfastμ-controllers,theelectrodepullerusesthelatest available technology for the controlof theheating furnacemanagingup to50A in theplatinum-iridiumfilament.Mechanicalandthermalprotectionwillinsurestableandreproducibleconditionsforthepipettefabrication.Simpleandefficientholdingsystemsguarantyaperfectclampoftheglass.

Features

• Motion is controlled by a force-controlled DC motor coupled with a high-resolutionpositionsensing.

• Self-squeeze electrode holding allow efficient manipulation while preventing glassslippingorbreaking.

• Heatingfurnaceusingamechanicallyformedplatinumiridiumfilamentisprotectedinaspecialhousingallowingthermalandmechanicalprotection.

• A4.3inchestouchscreenisimplementedwithspecificuserinterface.• Easyandintuitiveprogrammingontouchscreenmenus• Pre-setpullingprograms,withpossibilityofadjustingparameters• Memoryforover1000customprograms,userpasswordprotected,storedonnon-volatile

memory.

Typical applications

• Patchpipettes• Two-electrodesvoltageclampforXenopusoocytesrecordings• Sharpelectrodes• Intranuclearinjection• XenopusorZebrafishoocytesorembryoinjections• Insectegginjection• Aspirationpipettes• Drugapplicationforpatchclamp


Installation Placetheinstrumentonaflatandstablesurface.

a) Connectthepowersupplytotheinstrumentb) Connectthecabletothepowersupplyandto110V-220Vpowerlinec) Pressthepowerswitchtoturnontheinstrument

Thecoverprotectingthepullingmechanism,electrodeholdersandheatershouldbeclosedwhentheinstrumentisnotinuse.

Programming

The user interface UniPixSmartPullisdesignedtobesimpletouseandeasytoprogram.Everyuserhasthechoicebetween3pullingmodes,eachprimarilydesignedforspecificusages.

Injection pipettes

Thismodeisdesignedtopulltheglassinthreeheating/pullingsteps.Thisisused,forexample,for

patchclamppipettesorshortshanks.

Fine tip

Thismodeisdesignedtopulltheglassintwoheating/pullingsteps.Thismodecanbeusedfor

makingdifferenttipsincludingsharptips.

Patch/HiClamp

Thismodewithasinglepullisdesignedformakinginjectionpipettes.


Anewuserissimplycreatedbyenteringanewname.Hewillberequestedtostorea4letterspassword.Thefactoryparametersforthethreemodesareautomaticallyloaded.Whentheuserisworkingintooneofthepullingmodes,hecanfreelymodifyanyparameterofthemodeinordertooptimizethepullingprocessaccordingtoisrequirements.Thenewparametersareautomaticallystoredforthisuser(unlesstheuserdecidetocancelmodifications).Ifthesameuserwantstohaveseveralvariantsofpullingparametersinthesamemode,hesimplycreatesanewuser.Morethan1000userscanbedeclaredoneachinstrument.

Figure1:OverviewofSmartPullprogramstructure

Commands Thefollowingcommandscanbeentererinsteadoftheusername:emptyspace guestordefaultmode(notethatthismodeisnotpasswordprotected)calibration usefultodeterminetheminimaltemperatureforagivenglassabout displayUPLsoftwareversionandinternalparametersdemo demomode(canbestoppedonlybyturningoffthepuller)

Using Smart Pull

Start up

• Turnontheinstrumentwiththepowerswitch• Aself-testrunsautomaticallyandindicatespossiblemalfunction• Enteryourusername(ifleftblank=guestmode)• Setyourpassword(firsttimethepasswordwillbeset,nopasswordinguestmode)• Repeatpassword

Figure2:Useridentificationandpasswordinputscreens


Micropipette fabrication Selecttheprogram(tipmode):

Figure3:Programselectionscreen

Afterselectingthemode,thedisplaywillswitchtothespecificprogram.Aniconrepresentseachstep.Thepresetvaluesareoptimizedforbestpullingconditions.StartdisplaysoftheSmartPullprogrammodes:

InjectionpipettesFineTipPatch/HiClamp

1step3steps5steps

Figure4:Startscreensofthe3mainprogrammodes

Ifnecessary,youcanadjustallparametersinallsteps.Ifyouwanttochangeaparameterinastep,clickonthecorrespondingicon(seenextparagraphformoredetailsonstepparameters).

Loading the capillary

• PressSTART• Openthecoveroftheinstrument• Verifythatthetwocarriersareinloadconfiguration(closetotheheater)• Opentheholders(1)• Carefullyplacethecapillaryintothecarriers,ascenteredaspossible• Closetheholderbypushingdownbothflaps.Makesuretopressenoughinordertokeep

thecapillarytighttothecarrier


Figure5:Placementoftheglasscapillaryontheholdersandtighteningofthecapillarybeforepulling

Figure6:Pullingprocesslaunchscreen

Pipette pulling

• Closethecoveroftheinstrument• PressPULL(theprogramcanbeabortedbypressingSTOP)• WaituntiltheprogramendsandshowstheLOADscreenagain• Openthecoveroftheinstrument• Carefullyopenthetwoholdersandtakethepulledcapillaries

Figure7:Imageofthefirstheating/pullingstep

Figure8:Imagelastheatingstepandcapillariesafterthelastpullingstep


Pulling next capillary

• Repeattheloadingandpullingoperationsasoftenasnecessary.• Whenyouhavefinished,youcanturnoffthemainswitchoftheinstrument.• Ifyouhavechangedparameters(seesectionbelow)thelastparametersareautomatically

storedfortheuser.

Pulling steps parameters Atypicalpullingstepisdescribedbelow:

• TheheatturnsonatthesetHeat• Waitbeforepulling• Thefilamentheatsup, theglasssoftens,andaweakpulldrawstheglassoutwiththe

programmedForceuntilitreachestheprogrammedPosition.• WhentheprogrammedPositionhasbeenreached,theheatturnsoff

Byclickingontheicon«step1(or3,5)»,youaccessthestepmenuinwhichyoucanaccessthepullingparameters:

• Heat: settheinputcurrentoftheheater• Wait: setthewaitingtimebetweenheatingandpulling• Force: setthepullingforce• Position: setthetargetpositionforpulling

Tochangeaparameter,clickontheparametervalueandusethekeypadtomodifythevalue.PressDonebuttonforgoingbacktomodeprogram

Figure9:Programpullingparametersadjustmentscreenandtypepad

Parametersvalues:• Heat: 0-999 arbitraryunits• Force: 0-999 arbitraryunits• Position: 0-999 arbitraryunits• Wait: 0-999 seconds


Cooling step parameters Aftereachpullingstep,theprogramisexecutingacoolingstep.Byclickingontheicon«step2(or4)»,youaccessthestepmenuinwhichyoucanaccesstheCoolingtimeparameter:

Figure10:Programcoolingtimesettingscreen

Parametervalues:• Coolingtime: 0-999 secondsPresetvaluesofthetipmodesInjectionpipettes:

Step# Heat Wait[s] Position Force

1 850 5 40 900

2 - 5 - -

3 700 5 135 800

4 - 5 - -

5 550 0 800 600

FineTip:


1 870 5 140 600

2 - 5 - -

3 480 5 800 800

Patch/HiClamp:


1 950 3 800 800

Note:everytimeyoucreateanewuser,youwillhavethethreepreprogrammedtipmodes.


Calibration Theautocalibrationprogramiscalledbyenteringthecommand“calib”insteadoftheusername.Thecalibrationprogramisdesignedtohelptheusertodeterminatethepullingparameterswhenusingnewglasscapillariesorafterreplacingtheheatingfilament.RuntheCalibrationwhen:

• Youchangethefilament• Lotnumbersorcapillarytypeschange• Youcreateormodifyaprogram

Procedure:

1) Onstartingthepullertypecalibrationastheusername2) Mountaglasscapillary3) Press theSTARTkey to run thecalibration programTheheatingpower increases

gradually.Theheatingstopswhentheglassbeginstomove.4) Thevalueofheatingpowerisdisplayed.Thisisthebaselineheatingvalueforagiven

glasstypetested.Itisagoodstartingpointforthefirstcycleofyourprogram.

Figure11:Autocalibrationstartscreen

Connectivity (this feature is not activated in the current product)

SmartPullhasBluetoothandWiFiconnectivity.Allstoredparametersaswellasthelogofinstrumentusagewillbeaccessiblefromacomputeroratablet.Theprogramsandapplications forSmartPullaccesswillbedownloadedonUniPixwebsitewhenthefunctionalitywillbereleased.


Maintenance

Cleaning Routinelycleantheexteriorandthebaseplateoftheunitbywipingthemwithadryclothtoremovedustandfinepiecesofglass.Avoidcontactwiththefilament.If you break glass capillaries into the heater, you can simply remove fragments and dust byblowingairwithanairgunonthefilament.

• Don’tusetoohighairpressure• Don’ttouchthefilamentwithanobject• Weargogglestoprotectyoureyesforglassfragmentswhenyoublowair.• Itisnotrecommendedblowairwithyourmouth.

Replacing the O-ring of the glass capillary holder TheholdershaveanelastomerO-ringtoclampandavoidslidingoftheglasscapillaryduringthepulling.Ifyouobservebadclamping,thismaybeduetodamagedO-ring.Inthiscase,itisrecommendedtoreplacetheO-ringsofthetwoholders.OnlyuseO-ringssoldbyUniPix.O-ringreplacementsteps:

1. TurnOFFthepowersupply2. Removethescrew(1)3. Removetheholderflap,butkeepthesmalltubeinside(2)4. RemovetheoldO-ring(3)andcarefullyplacethenewO-ring5. Screwtheholderflap

Figure11:PictureofcapillaryholderandO-ringlocation


Replacing Heating Filament Ifthefilamentisdamagedofbroken,itmustbereplaced.ItisrecommendedthatyousendtheSmartPULLtoUniPixtodothefilamentreplacement(pleaseaskforthecostofreplacement).Ifyouwanttodothereplacementyourself,pleasecarefullyfollowtheinstructionsbelow:

ONLYUSEthefilamentsprovidedbyUniPixOtherfilamentsmaynotproperlyworkanddamagetheinstrument.

SmartPullreplacementfilamentsareprovidedwithaplasticholderthatholdstheminplaceduringthereplacementprocedure.Pleasefollowcarefullytheinstructions.AvideotutorialwillsoonbeavailableonUniPixwebsite.Filamentreplacementsteps:1. TurnOFFthepowersupply2. Removethegridthatenclosestheheatingfilament(4screws)3. Loosenthe2x2clampscrewsthatholdthefilamentinplace,andthenslideouttheold

filament.4. Slipinanewfilamentandthenretightenthe2x2clampscrews.5. Reinstallthegridthatenclosestheheatingfilamentandretightenthe4screws

Figure11:Pictureofprotectiongridandfilamentafterremovalofthegrid

After replacing the filament, run thecalibration program. In caseof anydysfunction,pleasecontactUniPixserviceatinfo@unipix-lab.comComplementaryinformationonmaintenanceandFAQcanbefoundontheUniPixwebsite:www.unipix-lab.com/upl.php


Troubleshooting

Problem Possiblecause Solution

The puller displayremainsblank

Verify the power supplyconnectionand that theLEDison

Connectthepowersupplyinanotherplug

The puller displayremains blank butthe power switchLEDison

Display system is malfunctioning

Turn OFF the instrument with themain switch, wait 10 seconds, turnONagain.Shouldtheproblempersistcallrepresentative,orcontactUniPix

No display orfrozendisplay

Programbug Turn OFF the instrument with themain switch, wait 10 seconds, turnONagain.Shouldtheproblempersistcall representative, or contact theUniPixwebsite

Glassdoesnotpullwell

Wrongparameters Check the pulling parameters. It isrecommendedtorunthecalibrationprogram to help finding the goodheating/pullingparameters.

Wrongcapillarymaterial UsetherecommendedcapillariesThe glass capillaryslides on thecarrier

Grippernottightenough Tighten more the capillary bypushingtheholderflaps.

Dustorgreaseontheholders. Cleanwithaclothimpregnatedwithalcohol

The filament doesnotheat

Turn off the puller, wait 10secondsbeforeturningon. Ifthefilamentisbrokenortheconnection is loose this willbediagnosedbytheselftest.

Replace the filament only if anapparent damage of the platinum isobserved


Technical specifications

Instrument Dimensions 53cmx40.6cmx30cmWeight 2.75kg(withoutpowersupply)Electrical 115/230volts,50/60HertzpowerlinePower 120W,maxHeatingfilament Platinum/IridiumFilamentPullingForce 0-20N,electroniccontrolledTaperLength 1-10mmCapillaryODrange 0.6–3.0mmCapillaryLength 50mm(min)to150mm(max)Note:SmartPullisdesignedforborosilicateglasscapillaries,notforquartzoraluminosilicate

Glass Capillary & Heating Filament Specifications

Glass Capillary

SmartPullmicropipettepuller isdesignedforusewithaluminosilicate,borosilicateorotherlowermelting-pointglasstubingorrodrangingfrom0.6to3.0mmindiameter.Thisinstrumentdoesnotpullquartzglass.ExamplesofthespecifictypesandsizesofglassthatcanbeusedwithSmartPullarelistedintheUniPixwebsiteatwww.unipix-lab.com.Anyglasswithcomparabletechnicalspecificationscanbeused.

Heating Filament

ThetypeandsizeofglassthatyouchoosemayrequireaHeatingFilamentotherthantheoneinstalledinyourpulleratthefactory.PleaserefertotheHeatingFilamentsectionofthismanualtodeterminetheappropriatestyleandsizeoffilamentnecessaryforpullingthespecificglassyouwouldliketouse.ONLYUSEUNIPIXSUPPLIEDREPLACEMENTHEATINGFILAMENTSINTHISINSTRUMENT

Systematic instructions for replacing the Heating Filament can be found in theMaintenancesectionofthismanual.

Technical Support TechnicalsupportisprovidedbyUniPixbyEmailatinfo@unipix-lab-com.


Limited warranty

• UniPixSwitzerlandlimitsthewarrantyonthisinstrumenttorepairandreplacementofdefectivecomponentsfortwoyearsfromdateofshipment,providedtheinstrumenthasbeenoperatedinaccordancewiththeinstructionsoutlinedinthismanual.

• Abuse,misuse,orunauthorizedrepairswillvoidthiswarranty.• Warrantyworkwillbeperformedonlyatthefactory.• The cost of shipment bothways is paid by UniPix Switzerland during the first three

monthsthiswarrantyisineffect,afterwhichthecostistheresponsibilityofthecustomer.• Thelimitedwarrantyisasstatedaboveandnoimpliedorinferredliabilityfordirector

consequentialdamagesisintended.• Consumables(filaments)areexemptfromthiswarranty.

UniPixSàrl,Ch.Neuf11,CH-1028Préverenges,SwitzerlandEmail:[email protected]


UniPixSàrlCH.Neuf11

CH-1028PréverengesSwitzerland

Email:[email protected]

DECLARATIONOFCONFORMITY

We,UniPixSàrl,Ch.Neuf11,CH-1028Préverenges,Switzerland,declareundersoleresponsibilitythattheproduct:

SmartPull

To which this declaration relates is in conformity with the following standards or othernormativedocuments: Safety: EN61010-1:200And therefore, conformswith the protection requirements of Council Directive 89/336/EECrelating to electromagnetic compatibility andCouncilDirective 73/23/EEC relating to safetyrequirements.

Issuedon: August10,2018_______________________________________ _______________________________PhilippeRenaud DanielBertrandPresident,CEO Vice-president,CTO

SmartPull manual 1 02 - Warner Instruments...2018/08/10 · UniPix Smart Pull Operation manual Rev....

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Transcript of SmartPull manual 1 02 - Warner Instruments...2018/08/10 · UniPix Smart Pull Operation manual Rev....