SLO: Apply previously learned technique to life cultures Ex. 3: Staining Techniques - Gram Stain,...

• SLO: Apply previously learned technique to life cultures

Ex. 3: Staining Techniques - Gram Stain, part 2 (Live Cultures)

How to label a slide

Circles on backOther info on front

“Little Finger Technique”

Smear Preparation

From Liquid Medium

• Suspend bacterial sediment in culture tube by gently tapping on it.

• Transfer one loopful of the bacterial suspension to slide and spread out (You may exceed the limits of the circle)

• Air-dry and heat-fix

From Solid Medium

• Transfer one loopful of H2O into circle area of slide.

• Using loop, suspend very small quantity of colony of interest in that drop of water and spread the suspension evenly. It should only appear slightly milky. Do NOT use too many bacteria

• Air-dry and heat-fix

Gram Stain Review

cheek cell in the center covered by various microorganisms present in dental plaque

Artifacts Precipitated crystal violet stain may resemble cocci. Clues: dense clumping, small size of the gram-positive dots. Paucity of organisms elsewhere in the field.

Examples of Gram Stains

Gram Stain of Urine

SLO: Apply previously learned technique to life cultures Ex. 3: Staining Techniques - Gram Stain,...

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