Microsoft Office 2010: Hands On James Crowley C3 – Crowley Computer Consulting.
Slide Preparation D. Crowley, 2007. Slide Preparation To be able to prepare a microscope slide...
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Transcript of Slide Preparation D. Crowley, 2007. Slide Preparation To be able to prepare a microscope slide...
Slide PreparationSlide Preparation
To be able to prepare a microscope slideTo be able to prepare a microscope slide
Tuesday, April 18, 2023
Microscope CrosswordMicroscope Crossword
Have a go at the microscope crossword - you have five minutes…
Down: -
1. Lens that you look down
2. What the slide is placed on
3. A wheel to make the image clear
Across: -
4. Lens closest to the slide
5. The specimen is placed on this
6. You need this to see something
Have a go at the microscope crossword - you have five minutes…
Down: -
1. Lens that you look down
2. What the slide is placed on
3. A wheel to make the image clear
Across: -
4. Lens closest to the slide
5. The specimen is placed on this
6. You need this to see something
EyepieceStageFocusing
ObjectiveSlideLight
MicroscopesMicroscopes
Set up a microscope to view an animal cell - remember the steps to take
Place the smallest objective lens (the smallest lens) over the hole in the stage Turn the coarse focusing wheel to make the gap between the stage and the
objective lens as small as possible Place a slide on the stage, and secure with the clips Adjust the light source so light goes up through the stage Turn the coarse focusing wheel slowing, until your specimen is in focus To see your specimen in more detail, turn the next largest objective lens over your
specimen Use the fine focusing wheel to get your image into focus again - do not use the
coarse focusing wheel - this can break your slide!
Set up a microscope to view an animal cell - remember the steps to take
Place the smallest objective lens (the smallest lens) over the hole in the stage Turn the coarse focusing wheel to make the gap between the stage and the
objective lens as small as possible Place a slide on the stage, and secure with the clips Adjust the light source so light goes up through the stage Turn the coarse focusing wheel slowing, until your specimen is in focus To see your specimen in more detail, turn the next largest objective lens over your
specimen Use the fine focusing wheel to get your image into focus again - do not use the
coarse focusing wheel - this can break your slide!
Preparing SpecimensPreparing Specimens
A specimen is the object we look at under a microscope
The specimen needs to be thin, so light can pass through it
We use a coverslip between the slide and specimen which helps flatten it out. It also helps keep the specimen in place, and stops it drying out
When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:
Magnification = magnification eyepiece lens x magnification objective lens
A specimen is the object we look at under a microscope
The specimen needs to be thin, so light can pass through it
We use a coverslip between the slide and specimen which helps flatten it out. It also helps keep the specimen in place, and stops it drying out
When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:
Magnification = magnification eyepiece lens x magnification objective lens
Preparing OnionsPreparing Onions
You are going to prepare a slide containing a specimen of onion cells
Follow the slide preparation steps, then draw what you see (using A4 plain paper and in pencil). Also write down your magnification
Magnification = magnification eyepiece lens x magnification objective lens
You are going to prepare a slide containing a specimen of onion cells
Follow the slide preparation steps, then draw what you see (using A4 plain paper and in pencil). Also write down your magnification
Magnification = magnification eyepiece lens x magnification objective lens
Place a drop of water on the centre of a slide
(this may contain a stain to make the
specimen more clear, e.g. iodine)
Cut some onion, then using forceps, peel off
the inside layer of a piece of onion
Place your onion skin onto the water droplet
on your slide
Using a mounted needle, lower a
coverslip onto the onion skin. Do this
slowly and carefully, to avoid trapping air
bubbles!
MagnificationMagnification
When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:
Magnification = magnification eyepiece lens x magnification objective lens
E.g. eyepiece lens = 10xobjective lens = 60x
Magnification = 10 x 60
= 600x magnification
When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:
Magnification = magnification eyepiece lens x magnification objective lens
E.g. eyepiece lens = 10xobjective lens = 60x
Magnification = 10 x 60
= 600x magnification