SKIN HEALTH AND

BEAUTY INGREDIENT

COLLAGEN PEPTIDES

Juan Manuel La Camera

page 2

Beauty from within

Nutricosmetic market and trends Beauty from within

Skin aging and collagen Mechanism of action

Clinical studies confirm - anti-

aging effects

Market products & LATAM

UK AND BRAZIL LEAD FOR COLLAGEN BEAUTY NPD

Source: Innova

Nutri-Cosmetics = Ingestible Beauty supplements & food/drinks

page 4

A variety of factors is driving sales of nutricosmetics

Growth in Nutricosmetics

Aging population

Societal factors Spa culture

Increasing consumer awareness

Shift toward less invasive procedures

Environmental factors

Global market for nutricosmetics

page 5

Source: Euromonitor

The ‘beauty from within’ concept is well established in Japan/Asia, with fish collagen as a key ingredient…

…and the market is growing in Europe and Americas…

Following the Asian Health & Beauty approach

Japan: Shiseido The Collagen Drink Three bottles of low molecular fish

collagen drink with eucommia ulmoides tea, ginseng, pearl-barley,

GABA, ornithine, hyaluronic acid, ceramide, royal jelly, vitamin C.

Most popular sales channel Convenience stores; Lawson, FamilyMart, 7-Eleven

7

Ingredient awareness amongst UK Consumers!

Source: Survey, SenseReach, UK, +1500 consumers, 70 pct female

CONSUMERS BELIEVE THAT DIETARY CHANGES CAN HELP TO IMPROVE SKIN HEALTH

Source: Innova

Top beauty claims on products:

Anti-aging anti wrinkle

Elasticity of the skin

Flexibility of the skin

Stronger/firmer: skin, hair & nails

Supplements is still the biggest market category for beauty from within, however healthy living positioned F&B launches are increasing.

HEALTHY LIVING POSITIONING INCREASING

Source: Innova

BEAUTY SUPPLEMENT RETURNING TO GROWTH

• Consumers that choose nutricosmetics products prefer those having the highest degree of natural image.

• Nutricosmetics are perceived as complementary to their current skincare routine

• Consumers that purchase nutricosmetics product, trust brands that are strong in nutraceutical fields

• People who are already used to consuming food supplements are more willing to try nutricosmetics

• The products should be adapt to consumers’ everyday habits and enable daily supplementation to be easier and more pleasurable

CONSUMER BEHAVIOR TOWARDS NUTRICOSMETICS

Source: Target Research, Euromonitor

The notion of ‘beauty from within’ is taken rather seriously in the Asian market. The addition of collagen to dried fruits, yogurt drinks, and other foods is a trend that has immense potential and has been driving food and beverage manufacturers to up their game and venture into the ever-transforming beauty industry.

NUTRICOSMETICS MARKET IN THE EAST TAKES THE LEAD

Source: Transparency market research, Euromonitor

Although confectionery accounts for almost 1/3 of all product launches tracked with skin health claims associated with collagen, the highest share of skin health claimed products is seen for beverages with a big focus on soft drinks.

BEVERAGES LEAD FOR COLLAGEN SKIN HEALTH CLAIMS

Source: Innova

In North America, capsules, tablets and gummies are dominating the market, while the supplements in Asia include powder, liquid, and jelly.

SUPPLEMENTS: ASIA VS NORTH AMERICA

Source: Innova Analysis

In Asia, especially Japan functional food delivery forms are popular among consumers. In Europe, UK is leading the trend and in LATAM, Brazil also has many collagen food product launches

Bella Berry Summer Fruit Beauty Drink (UK, Dec 2015)

Claim: Dietary supplement for joints for runners and cyclists. Regenerates ligaments, tendons and articular surfaces. Reduces friction during movement. Limits pain in the joints. Supports recovering from injury.

DELIVERY FORMS- FUNCTIONAL FOOD

Morinaga Collagen 100000 Collagen Drink (Japan, Nov 2014)

Claim: Morinaga collagen drink in peach flavor with high concentration collagen (10, 000mg) blended with"ceramide" for moisturizing.

Beauty Drink Abacaxi Com Hotela & Maracuja (Brazil, Jun 2015)

Claim: Rich in vitamins A, B1, B6 and B12.0% fat, sugar and sodium. With collagen. Gluten free. Recyclable packaging. Brazil anniversary edition. Collagen is protein engaged in firmness and elasticity of the skin. Beauty'in formula specifically developed to meet vitamins.

Multiple active health claims

Source: Innova Analysis

Beauty Drink Abacaxi Com Hotela & Maracuja (Brazil, Jun 2015)

Claim: Rich in vitamins A, B1, B6 and B12.0% fat, sugar and sodium. With collagen. Gluten free. Recyclable packaging. Brazil anniversary edition. Collagen is protein engaged in firmness and elasticity of the skin. Beauty'in formula specifically developed to meet vitamins.

The variety expands to everyday food- ambient dessert, bread, chocolate, or gummy…

DELIVERY FORMS- FUNCTIONAL FOOD 2 (COLLAGEN BASED MOBILITY CLAIMS USED CROSS CATEGORY)

Moderna Benefit Pan Integral Con Colageno (Ecuador, Oct 2015) Claim: …Benefits of collagen and sunflower seeds: collagen is a protein molecule that helps in the structure, elasticity and integrity of organs such as skin, hair, nails and joints, and sunflower seeds prevent the disintegration of collagen in the body.

Universal Gelatina Diet Extra Colageno Sabor A Fresa (Peru, Jun 2015) Claim: …Collagen protein that take care of the body and health. Nourishes, protects and helps maintain healthy and strong bones. Moisturizes and strengthens the skin. Prevents hair loss. It reduces pain caused by arthritis, slowing the process of osteoporosis and maintains healthy joints.

Sinnipai Collagen Jelly (Japan, Feb 2014) Claim: Improves the rough and dry skin. Widely used for healthy and beautiful body. Collagen jelly is a beauty ingredient for beautiful skin. Contains 15, 400mg of collagen. Can be eaten easily.

On the go format is particularly popular in Asia and arriving in UK too

Source: Innova Analysis

Health claims – Examples in the context of EFSA

Which claims can be used in the marketing of Collagen peptides today? Bone health

Protein contributes to the maintenance of normal bones • Protein is needed for normal growth and development of bone in children (art. 14)

Condition: source of protein (12% of the energy)

Joint health – none But with Vit C: Contributes to normal collagen formation for the normal function of cartilage

Muscle mass Protein contributes to growth in muscle mass Protein contributes to the maintenance of muscle mass Condition: source of protein (12% of energy)

Skin Beauty claims, -more complex situation, interpretation of EFSA scope A claim referring to a function on the body or the skin would fall under the scope of the regulation. “beauty” claims with no reference on health or functional properties should be excluded

Example of beauty claims -Enhance skin beauty, -Help to fight against the visible signs of skin ageing

page 18

Beauty from within

Nutricosmetic market and trends Beauty from within

Skin aging and collagen Mechanism of action

Clinical studies confirm - anti-

aging effects

Market products & LATAM

page 19

Collagen and elastin create skin’s structure, collagen provides

infrastructure, while elastin allows skin to stretch. Collagen also traps moisture, hyaluronic acid

During aging, decreased fibroblast (skin cells) activity

slows collagen+elastin synthesis, leading to loss of collagen and fragmentation: Dehydration Wrinkle formation Loss of firmness Loss of elasticity

Maintaining a well structured collagen network is the key to youthful skin

Skin aging : The important role of collagen

Peptides stimulates Collagen production Ex-vivo study -skin explants

page 20

Human skin explants in culture media Addition of collagen peptides to the media stimulated collagen production Increase in collagen density: - Collagen peptides treated sample (top) - compared to control (bottom), by staining collagen fibers with green/blue colour (day 9) (from Bio EC lab., France)

Collagen peptides sample

Morphology of a skin explant sample treated with Peptan. The density of the collagen fibers (green color) is enhanced.

Stratum corneum

Epidermis

Collagen structure (with Collagen peptides)

Papillary dermis

Untreated control explant sample with less dense coloration due to less collagen present.

Stratum corneum

Epidermis

Collagen structure (with control)

Papillary dermis

Control sample

page 21

Collagen peptides stimulates Hyaluronic acid (HA) production

Ex vivo: Addition of Collagen peptides to the media stimulated and increased the level of Glycoaminoglycans (main component= HA): Acidic GAGs are more evident (alcian blue colour) in the epidermis of the Peptan sample, compared to control (day 9). GAG increase by image analysis: +745 % !

(Bio EC lab, France. Rousselot)

Collagen peptides sample

(P2)

Control sample (T)

page 22

Beauty from within

Collagen peptides

Nutricosmetic market and trends Beauty from within

Skin aging and collagen Mechanism of action

Clinical studies confirm anti-

aging effects

Market products & LATAM

page 23

1- Production of collagen: Hydrolyzed collagens have an effect on the cells of connective

tissues The main hypothesis of action is that peptides coming from the oral intake

of collagen may be recognized as a fake signal of the destruction of endogenous collagen

Two Japanese studies show that ingestion of collagen peptides increases the size of the collagen fibrils, (Minaguchi, 2005) and enhances formation of the collagen fibrils (Matsuda, 2006).

Collagen Peptides

BLOOD

Specific Collagen derived peptides

Gly-Glu, Pro-Hypro

recognized by the cells as local collagen destruction

Activation of Skin cells

Local production of collagen by the body

Mechanism of action of collagen peptides

page 24

Collagen

Peptides

fibroblasts proliferation +

collagen production +++

hyaluronic acid synthesis +++

activation of the enz. HAS2 +++

Mechanism of action of collagen peptides

Hydration

Anti-aging

page 25

Beauty from within

Collagen peptides

Nutricosmetic market and trends Beauty from within

Skin aging and collagen Mechanism of action

Clinical studies confirm anti-aging effects

Market Products & LATAM

page 26

Successfully tested by leading skin research centers SOUKEN institute in Tokyo DERMSCAN institute in Lyon COSDERMA laboratories in Bordeaux >150 women participated Placebo controlled studies Latest advanced skin analysis

Scientifically proven Anti-age benefits - in just one month!

Placebo controlled Clinical studies carried out in Japan and France confirm the anti aging effect of taking Peptan®

Benefits are proven in clinical skin studies

YouthfulSkin

Increase moisture

Denser collagen

Smooth supple

skin

Collagen restructure

Reduce wrinkles

page 27

Objectives Assess the efficacy of Collagen peptides after 4 and 8 weeks during winter,

and in particular: Cutaneous water content Self-assessment by volunteers

Double-blind placebo-controlled trial of Oral supplementation of Collagen peptides in improving skin condition. Souken, Tokyo, Japan, 2008, Study YNTKK – 2008 – 4144

Japanese clinical study- SOUKEN

Study design 33 volunteers (40-59 years old women) Normal to dry skin Double blind vs placebo (dextrins) 10g of Peptan once a day in 100ml drink 8 weeks (October – December)

page 28

Objectives Assess the efficacy of Collagen peptides after 6 and 12 weeks during winter

Anti-wrinkle effect & Biomechanical properties Self-assessment

Evaluation of the effects of an oral intake of Collagen Peptides on cutaneous properties versus placebo. Dermscan, France, 2008 - Study 2008 – A00654 – 51

French clinical study- DERMSCAN

Study design 47 volunteers (35-55 years old women) Normal to dry skin Double blind vs placebo (dextrins) 10g of Peptan / day ( 2 x 5g powder) 12 weeks (October – January)

page 29

Study YNTKK – 2008 - 4144

Increases skin hydration

Increases skin hydration by 28% after 8 weeks

The skin hydration level of 91% of Collagen peptides group volunteers increased after 8 weeks

page 30

The number of deep wrinkles significantly increases with the placebo between baseline and 12 weeks: +30%

Study 2008 – A00654 - 51

Prevents deep wrinkle formation

page 31

Objectives Investigation of skins collagen structure using the latest advanced

technology incl. laser, – to demonstrate the underlying skin improvements leading to visible anti-age effects

Clinical study- Cosderma, France, 2012

Study design 106 volunteers , 40-65 years old women, Caucasian Double blind vs placebo, treatments given as powder drink Daily dosage of 10g in the morning Duration of 84 days. Materials Fish collagen peptides Maltodextrins (placebo).

Effect of oral ingestion of fish collagen peptides versus placebo on skin properties assessed in vivo by visual and laser scanning technology

Collagen fragmentation is a key indicator of skin aging !

page 32

Illustration of increased fragmentation of collagen with age (source ORION CONCEPT, Tours, France)

25 years old 68 years old

The information in this presentation cannot be reproduced without the permission of Rousselot

page 33

* p < 0.05 ** p < 0.001

*

**

Collagen peptides improves skin structure, by decreasing collagen fragmentation (Vivascope® confocal laser results)

Improves skin structure, - reversing the effect of aging!

Collagen fragmentation decreases significantly in the deep layers of the skin with collagen peptides: - 31 % . Significant effect after just 1 month

Study Cosderma 2012, in press

page 34

** **

Collagen peptides increases collagen density (Dermcup® high resolution echogenicity)

Increases collagen density - boosting skin from the inside

Collagen density was significantly increased after just 1 month with Collagen peptides: + 9 %

Study Cosderma 2012, in press

** p < 0.001

page 35

Beauty from within

Collagen peptides

Nutricosmetic market and trends Beauty from within

Skin aging and collagen Mechanism of action

Clinical studies confirm anti-aging effects

Market products & LATAM

page 36

FRENCH SECRET

Beauty secret from within US What is interesting?

15 natural ingredients: Collagen, Vitamin C, Alpha Lipoic acid, Fish oil fatty acids, ….

natural substances designed to support skin, hair and nail health 90 capsules - 3 capsules a day

WRINKLE Remedy

Dietary Supplement US What is interesting?

Collagen, Vitamins A, C, E and Minerals “For younger-looking skin”

60 Count

Beauty

Beauty Dietary supplements from the WW market

page 37

page 38

page 39

Id 1275948 1276372 (source-www)

Compañía SAPANAN SINNIPPAI YAKUHIN SkinGain _Med-eq

País Tailandia Japón Noruega

Lanzamiento 07/2012 07/2012 2012

Marca St. Anna Collagen Drink Sinnippai Collagen Jelly Peach Aji: Stick Shaped Collagen Jelly with Peach Flavor

SkinGain

Descripción del producto

Bebida de colágeno, en botella de plástico

Siete sticks de gomitas de colágeno, sabor durazno. Presentación pouch.

Skingain es un suplemento en polvo saborizado.

Claims Certificado halal. Colágeno 2,000mg. Suplemento dietario.

Contiene15400mg de péptidos de colágeno de pescado, 105mg N-acetilglucosamina, 52.5mg condroitina, 10.5mg ácido hialurónico y 25.2mg royal jelly extract.

Contraataca el proceso de envejecimiento de la piel. Skingain contiene Peptan combinado con otro colágeno de pescado. Cada sachet contiene 5000 mg de péptidos de colágeno.

Detalles

Fuente: Innova database 2012

Recent market launches

page 40

Id 1248419 1275566 1260075

Compañía KIM HENG ULTRA PRIMA ARTABOGA NATURES BOUNTY

País Tailandia Indonesia Brasil

Lanzamiento 05/2012 07/2012 06/2012

Marca Jelly Beautie Collagen Juice Drink with Mixed Fruit Flavor

OT Vitacharm Collagen Multi Probiotic Drink

Natures Bounty Collagen Dietary Supplement 90 Tablets

Descripción del producto

Jugo bebible con mix de sabores frutales, en envase doy pouch.

Cinco botellas individuales de bebida de colágeno y probióticos.

Colágeno hidrolizado con Vitamina C. El colágeno es una proteína importante para el crecimiento, reparación y mantenimiento de la piel, cabello, uñas y huesos.

Claims Contiene 60 calorías. Con 700mg de colágeno y plus de vitamina C.

Contiene 1,000mg de colágeno. Multi-probióticos ABC. Sin azúcar. Con Lactobacillus Acidophilus, Bifidobacterium, Lactobacillus Casei. Shot individual. Ready-to-drink.

La belleza comienza desde el interior. No contiene colorantes, saborizantes ni endulcorantes artificiales. No contiene conservantes.

Detalles

Recent market launches

page 41

Id 1272791 1237998 1247709

Compañía PURITANS PRIDE MEIJI KANRO

País United States Thailand Japan

Lanzamiento 06/2012 05/2012 05/2012

Marca Puritans Pride Ultra Woman Collagen Support with Hyaluronic Acid and Resveratro: 90 Capsules

Meiji Yoghurt Beauti Brightt: Yogurt with Crystal Collagen Beads

Kanro Pure Gummy Himitsu No Koi Aji: Gummy

Descripción del producto

Puritan's Pride presenta Ultra Woman Collagen Support con ácido hialurónico y Resveratrol. Productos de alta calidad y nutritivos a precios accesibles.

Cuatro vasos de yogurt bajo en grasas con 0.8% perlas de cristales de colágeno.

Gomitas con sabor oliva. Presentación en envase plástico con cierre.

Claims No contiene colorantes, saborizantes ni edulcorantes artificiales. Sin azúcar ni preservantes. No contiene almidón, leche, lactosa, soja. No contiene trigo, levaduras ni pescado. Libre de sodio.

Bajo en grasas y azúcar. Alto contenido de calcio y fibras. Contiene Colágeno 1120mg. Certificado halal y HACCP.

Sabor secreto del amor. Contiene extracto de amor. Edición limitada. Contiene vitamina C y colágeno.

Detalles

Recent market launches

page 42

Id 1184479 1249834 1158841

Compañía TC UNION GLOBAL TC UNION GLOBAL SAMYANG

País Tailandia Tailandia Korea del sur

Lanzamiento 02/2012 05/2012 12/2011

Marca Blink Collagen Drink Blink Collagen & Co Q10 Drink Samyang BDlab Beauty Aid Soft Drink

Descripción del producto

Seis botellas de bebida de colágeno, en caja de cartón.

Seis botellas de colágeno con colágeno y Co Q10, en cajas de cartón.

Bebida con ácido hialurónico y colágeno, para tratamiento de belleza.

Claims Colágeno 2,000mg. Sin azúcar agregada. Colágeno proveniente de Francia. Certificado GMP y Halal.

Certificado halal. Sin azúcar agregada. Alto contenido de vitaminas C y E.

Contiene ácido hialurónico y colágeno. Extracto de acerola adicionado.

Detalles

Recent market launches

page 43

Id 1297414 1323096 1321057

Compañía MEIJI TROPICAL OASIS DK HEALTH

País Japón Estados Unidos Tailandia

Lanzamiento Aug 2012 Oct 2012 Oct 2012

Marca Meiji Shokkans Gummi Gunittu Cola Aji: Hard Gummies with Cola Flavor

Tropical Oasis Sleep-A-Weigh Plus Dietary Supplement

NatureGift Instant Coffee Powder with Collagen, Vitamin & Minerals

Descripción del producto

Gomitas duras sabor cola, en envase plástico (pouch) con cierre.

Tropical Oasis Sleep-A-Weigh ayuda la reconstitución muscular, aumenta el metabolismo y combate el envejecimiento, todo esto mientras duermes! El colágeno es una proteína estructural que constituye el tejido conectivo, incluyendo tendones, ligamentos, músculos y cartílagos, que el cuerpo repara y reconstruye mientras duermes. Además, se trata de un nutriente que aporta fuerza y engrosamiento de la piel, cabello y uñas.

Cinco sachets de café instantáneo en polvo con colágeno, vitaminas y minerales.

Claims Contiene 2800mg de colágeno. Con vitamina C, vitamina B12 y colágeno. Certificado halal.

Detalles

Recent market launches

LATAM Q4 2014 to date

LATAM Q4 2014 to date

LATAM Q4 2014 to date

Peptan® collagen peptides page 47

Peptan® Collagen Peptides

Rousselot® technology based on a specific enzymatic hydrolysis process Excellent organoleptic profile Product consistency

2000 – 5000 Da Highly digestible Soluble in cold water

Peptan® is a bioactive ingredient characterized by a unique amino acid composition and high bioavailability

Collagen type 1 peptides high in the AA’s Glycine, Hydroxyproline, Proline and Glutamic acid

Peptan® collagen peptides page 48

Broad range to meet all customer needs Peptan® is available in different standard grades,

raw material, molecular weight, and powder specifications

Peptan P is Porcine collagen peptides Peptan B is Bovine collagen peptides Peptan F is Fish collagen peptides

HD (High density): 0.4 - 0.55 g/cm3 - LD (Low density): 0.25 - 0.35 g/cm3 /EU Pharma grade Peptan® is also available.

Peptan® product range

Peptan® collagen peptides

Peptan® – scientifically proven bioactive peptides

Building blocks for innovative products

Beauty from within

Healthy Aging & Mobility

Sports/Fitness & Senior Nutrition

Peptan® Péptidos de colágeno 50

www.peptan.com

Péptidos de colágeno para

un estilo de vida saludable