SKIN COURSES FROM PART I TO IX
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Page1 SKIN COURSES FROM Part (I TO Part IX). SKIN, HISTOLOGICAL STRUCTURE. Part I. CE TRACKER# 20-655631. Authors: Carmen I. Perez HTL, BSc accredited by FIU, M.D. Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Sc. Created: 01/14/2019 Updated: 07/12/2020 Course Description: This is the first part of a series of third individual courses designated to excel in the area of work of dermatological specimens in the clinical laboratory of Pathology. This first course reviews all the normal histology of skin, and its appendages through the optical and electron microscope. Objectives: 1.0. Introduction to the skin, and its appendage. 1.1. Explain origin of the skin. 1.2. List and explain functions of the skin. 1.3. Identify and describe the different layers of skin 1.4. Identify and describe the epidermis. 2.0. Describe the histological structure of the dermis. 2.1. Describe the histological structure of the papillary layer. 2.2. Describe the histological structure of the reticular layer. 2.3. Describe histological structure of hypodermis. 3.0. List the skin appendages. 3.1. Introduction to hair structure and origin. 3.2. List parts of the hair. 3.3. Describe the histological structure of the hair. 3.4. List layer of the hair and describe them. 3.5. Describe cycle of hair growth. 4.0. Describe histology of sebaceous gland. 5.0. Describe histology of sweat glands. 6.0. Describe histology of nails CE credits: Total CE credit (1) Credits, 1 CE Histology Delivery method: Live, classroom Course hours: 1 hours.
Transcript of SKIN COURSES FROM PART I TO IX
SKIN COURSES FROM PART I TO IXSKIN COURSES FROM Part (I TO Part
IX).
SKIN, HISTOLOGICAL STRUCTURE. Part I. CE TRACKER# 20-655631. Authors: Carmen I. Perez HTL, BSc accredited by FIU, M.D. Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Sc.
Created: 01/14/2019 Updated: 07/12/2020 Course Description: This is the first part of a series of third individual courses designated to excel in the area of work of dermatological specimens in the clinical laboratory of Pathology. This first course reviews all the normal histology of skin, and its appendages through the optical and electron microscope. Objectives: 1.0. Introduction to the skin, and its appendage. 1.1. Explain origin of the skin. 1.2. List and explain functions of the skin. 1.3. Identify and describe the different layers of skin 1.4. Identify and describe the epidermis. 2.0. Describe the histological structure of the dermis. 2.1. Describe the histological structure of the papillary layer. 2.2. Describe the histological structure of the reticular layer. 2.3. Describe histological structure of hypodermis. 3.0. List the skin appendages. 3.1. Introduction to hair structure and origin. 3.2. List parts of the hair. 3.3. Describe the histological structure of the hair. 3.4. List layer of the hair and describe them. 3.5. Describe cycle of hair growth. 4.0. Describe histology of sebaceous gland. 5.0. Describe histology of sweat glands. 6.0. Describe histology of nails CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
Course hours: 1 hours.
• 10 questions • Passing score
• Clinical laboratory Histotechnician, Histotechnologist, Research technician, Biology laboratory personnel.
Content outline: 1.0. Introduction to the skin, and its appendage. 1.1. Origin of the skin. 1.2. Functions of the skin. 1.3. Histological Characteristic of the different layers of skin 1.4. Epidermis, characteristic histological of cells of skin. M/O and M/E. 2.0. Histological structure of the dermis, in papilar and reticular layer. 2.1. Describe the histological structure of the papillary layer. 2.2. Describe the histological structure of the reticular layer. 2.3. Describe histological structure of hypodermis. 3.0. List the skin appendages. 3.1. Introduction to hair structure and origin. 3.2. List parts of the hair. 3.3. Describe the histological structure of the hair. 3.4. List layer of the hair and describe them. 3.5. Describe cycle of hair growth. 4.0. Describe histology of sebaceous gland. 5.0. Describe histology of sweat glands. 6.0. Describe histology of nails Authors information: Carmen I. Perez.
Ms. Perez is a Senior Histotechnologist, Supervisor Florida at the Dermatopathology Laboratory of the Department of Dermatology and Cutaneous Surgery, University of Miami, Miller School of Medicine.
She attended Faculty Medicine, Havana Cuba and earned the degree of Doctor in Medicine in 1976; she graduated later as Specialist of the first level in Oncology 1982 Havana, Cuba.
Ms. Perez has a long work history in oncology, histology, cytology, and hematology which is supported by their multiple's publications, and presentations reflected in her curriculum vitae.
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She began working in the histology field as soon as she graduated first as Histology technician at School of Histology Technician, Havana, Cuba. 1963, working then at Dermatology Department of Hospital Fajardo Havana Cuba, later her experience leads her to teach medical students at Faculty of Medicine of Matanzas Cuba as Instructor of Histology later at Faculty Julio Trigo, Havana. BSc accredited in FIU Florida 1995, Histotechnologist and Supervisor in Florida since 1995 to 2009, respectively. She worked at Pathology Department of University of Miami, Fl, 1994, and later as Senior Histotechnologist, Supervisor Florida at the Dermatopathology Laboratory of the Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine. She has specialized in Special Stains, IHC, and technical Supervisor of grossing area.
Maritza R. Martinez
She is a freelance writer of histology courses, audio-visual aids, PowerPoint and website content. She is Manager of small business company MEDLAB educational & Research Services. LLC.
She has worked in Histology field since she graduated from Faculty of the Medicine University of Havana in 1970, Specialist of Histology first and second degree 1975 and 1992, respectively. She was appointed a Full professor of Histology at Faculty of Medicine1989-1993. Earned Ph.D. degree in Medical Science in Cuba in the field of Histology in 1981. She was appointed Chairperson of Histology Department, Faculty of Medicine, Havana Cuba in 1975-1979, 1980-1982, 1990-1992 a position that she held for nine years. In 1990 she was Head of the group of research of monoclonal antibody. She is Author of Histology, a textbook for medical students of Department of Histology Faculty of Medicine Havana Cuba, published in 1987 for Editorial Pueblo y Educacion and reprinted in 1990. Her experience as a lecturer in Histology, medical education for 23 years led her to create programs for medical students and residency programs for postgraduate in Histology residency in Cuba.
BSc accredited in FIU Florida 1995. Histotechnologist, and Supervisor in Florida since 1995.
Ms. Martinez worked al Pathology Department, at University of Miami as a cytotechnician in 1994, later she worked as Histotechnologist at Neurology Department at University of Miami, Miller School of Medicine in the Clinical Laboratory of muscle and nerve pathology, later as Senior Histotechnologist, Supervisor Florida 2010 at the Dermatopathology Laboratory of the Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine (retired) where she specialized in histochemistry, special stains and IHC.
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DERMATOLOGICAL SPECIMENS, REGULATORY AND SAFETY PROCEDURES FOR TRANSPORTATION, COLLECTION AND ACCESSIONING. Part II. CE TRACKER# 20-728294 Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/9/2019
Description:
The second part of this series of courses is dedicated to recognizing and describe the regulatory and safety procedures for dermatological specimens (collection. accessioning) This includes the understanding of quality assurance and quality control measures in dermatopathological laboratories.
It is known around the eighty percent of errors occurs in the reception and grossing area of the clinical laboratories.
A well-trained technical staff understands the principles and knowledge to perform the procedures established in the laboratory and how to proceed with the various specimens received in the dermatopathology laboratory, to maintain patient- specimen integrity and accuracy through the collection and accessioning of specimens.
Objectives: 1.0. Understand the principles of good laboratory practices (GLP). 1.1. Recognize and describe the regulatory and safety procedures for specimen
reception, identification, and handling of dermatological specimens. 1.2. Understanding of quality assurance and quality control measures in dermatopathology laboratory. 2.0. Understand the steps and care for specimen collection and transport. 3.0. Summarizes importance and action taken to maintain patient/specimen integrity and
accuracy through the collection of specimens. 4.0. Demonstrate knowledge of the principles of transportation of specimens, fixation, handling, and processing of dermatological specimens. 5.0. Describe how to proceed with special orders in dermatology as immunofluorescent order. 6.0. Demonstrate and exhibit positive professional attitudes at all time in the
laboratory, including confidentially and ethical decision-making.
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CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
• Clinical laboratory Histotechnician, Histotechnologist, Research technician, Biology laboratory personnel.
Content outline: 1. Understand the principles of good laboratory practices (GLP). 2. Recognize and describe the regulatory and safety procedures for specimen reception,
identification, and handling of dermatological specimens. 3. Understanding of quality assurance and quality control measures in dermatopathology laboratory. 4. Understand the steps and care for specimen collection and transport. 5. Summarizes importance and action taken to maintain patient/specimen integrity and accuracy through the collection of specimens. 6. Demonstrate knowledge of the principles of transportation of specimens, fixation, handling, and processing of dermatological specimens. 7. Describe how to proceed with special orders in dermatology as immunofluorescent, others. 8. Demonstrate and exhibit positive professional attitudes at all time in the laboratory, including confidentially and ethical decision-making. 9. Demonstrate knowledge of the principles of laboratory safety.
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SURGICAL GROSSING OF DERMATOLOGICAL SPECIMEN. Part III. CE TRACKER# 20-664318
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/9/2019
Description:
The third part of this series of courses is dedicated to update the basic function and management of the gross room, well trained personnel is needed in the reception and grossing area of the clinical laboratories to have the best understanding of the principles, the procedures and protocols established in the gross room area of the laboratory.
How to proceed with the various types of dermatological specimens received in the dermatopathology laboratory guarantee the proper handling and processing of the specimen to the correct diagnosis or treatment of the patient. Training in safety and exposure to dangerous pathogens, chemicals and other safety risk of the gross rooms are reviewed.
Objectives:
1. Summarize how to set up, operate and maintain instruments for gross room station.
2. List of instruments and supplies for room station.
3. Understand the anatomical terms used for orientation of tissues and inking of specimens for macroscopically and microscopic orientation.
4. Demonstrate knowledge of the principles of fixation and handling of dermatological specimens.
5. Describe the various specimens receive in the dermatology laboratory.
6. Describe the most common grossing protocol for biopsies shaves, punches and excisions.
7. Describe how excision are grossed with orientation and without.
8. Potential grossing errors, how to prevent them.
9. Demonstrate and exhibit positive professional attitudes at all time in the laboratory, including confidentially and ethical decision-making. HIPPA
10. Demonstrate knowledge of the principles of laboratory safety.
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CE credits: Total CE credit (1) Credits, 1 CE Histology Delivery method: Live, classroom
Course hours: 1 hours. Content: 10 pages Exam Information:
• 10 questions • Passing score
Content Outline
1. How to set up, operate and maintain instruments for gross room station.
2.0 List of instruments and supplies for room station.
3.0 Orientation of tissues and inking of specimens for macroscopically and microscopic orientation.
4.0 Fixation and handling of dermatological specimens.
5.0 Types of specimens receive in the dermatology laboratory.
6.0 Describe grossing protocols.
c. grossing protocol for excisions.
7.0 Excision are grossed with orientation and without.
8.0 Grossing potential error.
EMBEDDING IN PARAFFIN, DERMATOLOGICAL SPECIMENS. PART IV. CE TRACKER# 20-728828
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/10/2019
Description:
This course examines the proper embedding of standardized techniques for skin specimens to obtain optimal diagnostic slides from skin. It is known that the orientation of skin tissue in embedding is determined by the epidermis. The proper orientation has the most impact on the diagnostic, if the lesion area is cut away, a diagnosis is not possible. That is one of the reasons that some Histotechnician consider the skin embedding a challenge.
Objectives:
1. Summarize histology of skin, layers. 2. Outline general rules of tissue embedding. 3. Describe skin embedding techniques. 4. Recognize embedding artifact and how troubleshoot them. 5. List alternative embedding media.
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
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a. Epidermis
b. Dermis
a. Embedding in paraffin definition.
b. General embedding of a tissue in paraffin
c. Choosing the correct paraffin, mold and cassettes
d. Types of orientation a tissue of different organs.
3. Skin Embedding protocols.
4. Artifacts in the embedding process and how to avoid them.
5. Alternative embedding media.
CUTTING SECTIONS IN PARAFFIN, DERMATOLOGICAL SPECIMENS. PART V. CE TRACKER# 20- 728834.
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/10/2019
Description:
This course examines the proper cutting techniques for skin specimens to obtain optimal diagnostic slides from skin. It is known that the orientation and cutting sections has the most impact on the diagnostic, if the lesion area is cut away, a diagnosis is not possible. That is one of the reasons that some Histotechnician consider the skin cutting a challenge.
Objectives:
1. Summarize histology of skin, layers. 2. Outline general rules of tissue sectioning in paraffin. 3. Describe skin sectioning techniques. 4. Recognize sectioning artifacts and how troubleshoot them. 5. Describe other alternative cutting methods.
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CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
Content Outline:
a. Epidermis
b. Dermis
a. Choosing the correct knives.
d. Orientation of the skin during embedding.
3. Skin sectioning protocols.
4. Artifacts in the sectioning process and how to avoid them.
5. Alternative cutting sections.
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HAIR HISTOLOGY, AND INSTRUCTIONS FOR SCALP PUNCH BIOPSIES. PART VI. CE TRACKER# 20-728834
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/10/2019
Description:
Getting a proper scalp biopsy and following the stablished protocol steps during accessioning, grossing and embedding are important elements in diagnosing a patient with hair loss.
This course is oriented to review the histological structure of the hair, the importance of the diagnosis and care of technical staff of the request of the physician about the test to be done, which must be noted by technical personnel during accessioning, the surgical grossing techniques and embedding protocols of scalp punch biopsies. Recommendations for cutting sections of transversal punch biopsies are reviewed.
Objectives:
This course is oriented to review the histological structure of the hair, accessioning, embedding protocol for general skin punch biopsies and scalp biopsies. Recommendations of transverse cut sections of scalp hairs.
Intended audience:
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
Course hours: 1 hours. Content: 10 pages Content Outline: Content outline: 1.0. Anatomy of the hair and hair follicle 1.1. Origin of the hair 1.2. Histological Characteristic of the different layers of the hair follicle. 1.3. Hair follicles cycles of hair growth. 2.0. Describe histology of sebaceous gland. 3.0. Describe histology of sweat glands. 4.0. Accessioning, fixation of punch biopsy.
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4.1. Grossing protocols of punch biopsies of skin and scalp biopsy 4.2. Embedding protocols on punch biopsies of skin and scalp biopsy. 4.3. Cutting protocols of skin punch biopsies and scalp. NAIL HISTOLOGICAL STRUCTURE, ACCESSIONING, PROCESSING, CUTTING, AND STAINS FOR HISTOPATHOLOGY STUDIES, PART VII. CE TRACKER# 20-729012
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/10/2019
Description:
Nail disorders can arise at any age. About half of all nail disorders are of infectious origin, 15% are due to inflammatory or metabolic conditions, and 5% are due to malignancies and pigment disturbances. The differential diagnosis of nail disorders is an area of interest of histopathology. Malignant tumors of the nails are often not correctly diagnosed at first. For subungual melanoma, the mean time from the initial symptom to the correct diagnosis is approximately 2 years; this delay can be partly responsible for the low 10-year survival rate of only 43%. A correct histological evaluation of the nail tissue is an important diagnostic instrument. This course is designated to aim to improve the knowledge and skills of the technical personnel trough all steps of the nail histology procedure in the histopathology laboratory.
Objectives:
1.0. Describe the anatomy of the nail. 1.1. Summarize histological characteristic of nails. 2.0. Describe areas of pathological interest. 3.0. Accessioning and fixation of nail 3.1. Recommendations to avoid falling off the nail clipping. 4.1. List common regular and special stains used for nail histopathological studies.
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
Content Outline:
1.0. Anatomy of the nail. Parts 1.1. Normal histology of nails. 2.0. Areas of pathological interest in nails. Common pathologies. 3.0. Accessioning and fixation of nail recommendations 3.1. Recommendations to avoid falling off the nail clipping. 3.2. Recommendations for cutting nail embedded in paraffin. 4.1. List common regular and special stains used for nail histopathological studies.
DERMATOLOGICAL SPECIMENS, USEFUL SPECIAL STAINS USED IN THEIR DIAGNOSTIC. PART VIII.
CE TRACKER #: 20-729078 Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/12/2019 Description:
Diagnostic evaluation of disorders of skin tissue is largely based on a thorough examination of the sections stained with hematoxylin and eosin (H&E). Additional special stains may be used to highlight or identify features that are not easily seen on an H&E stain. The choice of stain or panel of stains depends on the findings on initial assessment, the clinical context, and the preference of the pathologist. Every patient may require several special stains for an optimal assessment. The intent of this course is to discuss the common stains used in the evaluation of dermatological specimens.
There are a lot of special stains available in the histopathology laboratory that can be used for the detection of microorganisms y/ or cellular components. Due to complexity of some of the special stain procedures and other factors, the quality of stain sometimes can be poor and no suitable tor diagnosis, this failure can be solved in dependence of the skills of the technical personnel. Some more advances laboratories have special staining automatic machines, making easier the task and reducing the TAT of the laboratory. However, all technical personnel must seriously consider having the manual skills of perform any special stain needed in the laboratory, this complete the qualification of the personnel. This participation in this course will be of aim to complete and update the knowledge and skills necessary in this important area of work.
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Objectives:
1.0. Understand staining methods used in the clinical histopathology for skin pathologies. 2.0. List special stains used in histopathological studies of skin tissue. 3.0. Describe purpose, methods and procedures of each of them. 4.0. Discuss more common factors than can affect the quality of special stain results and how troubleshoot them. 5.0. Demonstrate skills to follow a stain protocol and prepare working solutions.
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
Content Outline
1.0 Components of skin tissue and staining methods used in histopathology of skin
2.0 Special stains used in skin tissue studies.
2.1 CONECTIVE TISSUE: COLLAGEN; PTHA – Mallory, Masson Trichrome, Movat, Picric Sirious, H Gomori one-step trichrome, Van Gieson ELASTIC FIBERS: Verhoeff RETICULIN; Gordon&Sweet, PTHA MUCOPOLISACHARIDES: Alcian Blue, colloidal Iron. AMYLOID: Crystal Violet, Congo Red BACTERIA: B&B, AFB Ziehl-Nelsen, Fite -Faraco, Steiner, Mucicarmin FUNGUS: PAS, GMS, PIGMENTS: melanin, Fontana-Masson MINERALS: Iron, Von Kossa-Calcium.
3.0 Purpose, methods and procedures of each of stains listed above.
4.0 Factors that affect results and quality of special stains and how troubleshooting 4.1 Troubleshooting
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OVERVIEW OF IMMUNOHISTOCHEMISTRY (IHC) OF THE SKIN.
Part IX CE TRAKER #20-729286. Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/15/2019
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/12/2019
Description:
Diagnostic evaluation of disorders of skin tissue is largely based on a thorough examination of sections stained with hematoxylin and eosin (H&E). Additional IHC stains are used by the pathologist to highlight or identify features that are not easily interpret on an H&E stain. The choice of IHC stain or panel of stains depends on the findings on initial assessment, the clinical context, and the preference of the pathologist. Every patient may require several IHC stains for an optimal assessment. Some more advances laboratories have IHC automatic machines, making easier the task and reducing the TAT of the laboratory. However, all technical personnel must seriously consider having the skills to perform any IHC stain needed in the laboratory, this complete the qualification of the technical personnel. The participation in this course will be of aim to complete the knowledge and skills necessary in this important area of work.
The intent of this course is to discuss IHC stains used in the evaluation of dermatopathology specimens.
Objectives:
1.0. Understand staining methods used in the clinical histopathology for skin pathologies. 2.0. Explain factors that affect results and quality of IHC stain and how troubleshooting them. 3.0 List IHC stains used in skin tissue 4.0 Discuss more common factors than can affect the quality of IHC stain results.
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
Course hours: 1 hours.
• 10 questions • Passing score
Content Outline:
1.0. Staining methods used in the clinical histopathology for skin pathologies.
2.0. Factors that affect results and quality of IHC stain and how troubleshooting them.
3.0. IHC stains panels used in skin pathologies.
4.0. Common factors than can affect the quality of IHC stain results.
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SKIN, HISTOLOGICAL STRUCTURE. Part I. CE TRACKER# 20-655631. Authors: Carmen I. Perez HTL, BSc accredited by FIU, M.D. Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Sc.
Created: 01/14/2019 Updated: 07/12/2020 Course Description: This is the first part of a series of third individual courses designated to excel in the area of work of dermatological specimens in the clinical laboratory of Pathology. This first course reviews all the normal histology of skin, and its appendages through the optical and electron microscope. Objectives: 1.0. Introduction to the skin, and its appendage. 1.1. Explain origin of the skin. 1.2. List and explain functions of the skin. 1.3. Identify and describe the different layers of skin 1.4. Identify and describe the epidermis. 2.0. Describe the histological structure of the dermis. 2.1. Describe the histological structure of the papillary layer. 2.2. Describe the histological structure of the reticular layer. 2.3. Describe histological structure of hypodermis. 3.0. List the skin appendages. 3.1. Introduction to hair structure and origin. 3.2. List parts of the hair. 3.3. Describe the histological structure of the hair. 3.4. List layer of the hair and describe them. 3.5. Describe cycle of hair growth. 4.0. Describe histology of sebaceous gland. 5.0. Describe histology of sweat glands. 6.0. Describe histology of nails CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
Course hours: 1 hours.
• 10 questions • Passing score
• Clinical laboratory Histotechnician, Histotechnologist, Research technician, Biology laboratory personnel.
Content outline: 1.0. Introduction to the skin, and its appendage. 1.1. Origin of the skin. 1.2. Functions of the skin. 1.3. Histological Characteristic of the different layers of skin 1.4. Epidermis, characteristic histological of cells of skin. M/O and M/E. 2.0. Histological structure of the dermis, in papilar and reticular layer. 2.1. Describe the histological structure of the papillary layer. 2.2. Describe the histological structure of the reticular layer. 2.3. Describe histological structure of hypodermis. 3.0. List the skin appendages. 3.1. Introduction to hair structure and origin. 3.2. List parts of the hair. 3.3. Describe the histological structure of the hair. 3.4. List layer of the hair and describe them. 3.5. Describe cycle of hair growth. 4.0. Describe histology of sebaceous gland. 5.0. Describe histology of sweat glands. 6.0. Describe histology of nails Authors information: Carmen I. Perez.
Ms. Perez is a Senior Histotechnologist, Supervisor Florida at the Dermatopathology Laboratory of the Department of Dermatology and Cutaneous Surgery, University of Miami, Miller School of Medicine.
She attended Faculty Medicine, Havana Cuba and earned the degree of Doctor in Medicine in 1976; she graduated later as Specialist of the first level in Oncology 1982 Havana, Cuba.
Ms. Perez has a long work history in oncology, histology, cytology, and hematology which is supported by their multiple's publications, and presentations reflected in her curriculum vitae.
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She began working in the histology field as soon as she graduated first as Histology technician at School of Histology Technician, Havana, Cuba. 1963, working then at Dermatology Department of Hospital Fajardo Havana Cuba, later her experience leads her to teach medical students at Faculty of Medicine of Matanzas Cuba as Instructor of Histology later at Faculty Julio Trigo, Havana. BSc accredited in FIU Florida 1995, Histotechnologist and Supervisor in Florida since 1995 to 2009, respectively. She worked at Pathology Department of University of Miami, Fl, 1994, and later as Senior Histotechnologist, Supervisor Florida at the Dermatopathology Laboratory of the Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine. She has specialized in Special Stains, IHC, and technical Supervisor of grossing area.
Maritza R. Martinez
She is a freelance writer of histology courses, audio-visual aids, PowerPoint and website content. She is Manager of small business company MEDLAB educational & Research Services. LLC.
She has worked in Histology field since she graduated from Faculty of the Medicine University of Havana in 1970, Specialist of Histology first and second degree 1975 and 1992, respectively. She was appointed a Full professor of Histology at Faculty of Medicine1989-1993. Earned Ph.D. degree in Medical Science in Cuba in the field of Histology in 1981. She was appointed Chairperson of Histology Department, Faculty of Medicine, Havana Cuba in 1975-1979, 1980-1982, 1990-1992 a position that she held for nine years. In 1990 she was Head of the group of research of monoclonal antibody. She is Author of Histology, a textbook for medical students of Department of Histology Faculty of Medicine Havana Cuba, published in 1987 for Editorial Pueblo y Educacion and reprinted in 1990. Her experience as a lecturer in Histology, medical education for 23 years led her to create programs for medical students and residency programs for postgraduate in Histology residency in Cuba.
BSc accredited in FIU Florida 1995. Histotechnologist, and Supervisor in Florida since 1995.
Ms. Martinez worked al Pathology Department, at University of Miami as a cytotechnician in 1994, later she worked as Histotechnologist at Neurology Department at University of Miami, Miller School of Medicine in the Clinical Laboratory of muscle and nerve pathology, later as Senior Histotechnologist, Supervisor Florida 2010 at the Dermatopathology Laboratory of the Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine (retired) where she specialized in histochemistry, special stains and IHC.
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DERMATOLOGICAL SPECIMENS, REGULATORY AND SAFETY PROCEDURES FOR TRANSPORTATION, COLLECTION AND ACCESSIONING. Part II. CE TRACKER# 20-728294 Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/9/2019
Description:
The second part of this series of courses is dedicated to recognizing and describe the regulatory and safety procedures for dermatological specimens (collection. accessioning) This includes the understanding of quality assurance and quality control measures in dermatopathological laboratories.
It is known around the eighty percent of errors occurs in the reception and grossing area of the clinical laboratories.
A well-trained technical staff understands the principles and knowledge to perform the procedures established in the laboratory and how to proceed with the various specimens received in the dermatopathology laboratory, to maintain patient- specimen integrity and accuracy through the collection and accessioning of specimens.
Objectives: 1.0. Understand the principles of good laboratory practices (GLP). 1.1. Recognize and describe the regulatory and safety procedures for specimen
reception, identification, and handling of dermatological specimens. 1.2. Understanding of quality assurance and quality control measures in dermatopathology laboratory. 2.0. Understand the steps and care for specimen collection and transport. 3.0. Summarizes importance and action taken to maintain patient/specimen integrity and
accuracy through the collection of specimens. 4.0. Demonstrate knowledge of the principles of transportation of specimens, fixation, handling, and processing of dermatological specimens. 5.0. Describe how to proceed with special orders in dermatology as immunofluorescent order. 6.0. Demonstrate and exhibit positive professional attitudes at all time in the
laboratory, including confidentially and ethical decision-making.
Pa ge
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
• Clinical laboratory Histotechnician, Histotechnologist, Research technician, Biology laboratory personnel.
Content outline: 1. Understand the principles of good laboratory practices (GLP). 2. Recognize and describe the regulatory and safety procedures for specimen reception,
identification, and handling of dermatological specimens. 3. Understanding of quality assurance and quality control measures in dermatopathology laboratory. 4. Understand the steps and care for specimen collection and transport. 5. Summarizes importance and action taken to maintain patient/specimen integrity and accuracy through the collection of specimens. 6. Demonstrate knowledge of the principles of transportation of specimens, fixation, handling, and processing of dermatological specimens. 7. Describe how to proceed with special orders in dermatology as immunofluorescent, others. 8. Demonstrate and exhibit positive professional attitudes at all time in the laboratory, including confidentially and ethical decision-making. 9. Demonstrate knowledge of the principles of laboratory safety.
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SURGICAL GROSSING OF DERMATOLOGICAL SPECIMEN. Part III. CE TRACKER# 20-664318
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/9/2019
Description:
The third part of this series of courses is dedicated to update the basic function and management of the gross room, well trained personnel is needed in the reception and grossing area of the clinical laboratories to have the best understanding of the principles, the procedures and protocols established in the gross room area of the laboratory.
How to proceed with the various types of dermatological specimens received in the dermatopathology laboratory guarantee the proper handling and processing of the specimen to the correct diagnosis or treatment of the patient. Training in safety and exposure to dangerous pathogens, chemicals and other safety risk of the gross rooms are reviewed.
Objectives:
1. Summarize how to set up, operate and maintain instruments for gross room station.
2. List of instruments and supplies for room station.
3. Understand the anatomical terms used for orientation of tissues and inking of specimens for macroscopically and microscopic orientation.
4. Demonstrate knowledge of the principles of fixation and handling of dermatological specimens.
5. Describe the various specimens receive in the dermatology laboratory.
6. Describe the most common grossing protocol for biopsies shaves, punches and excisions.
7. Describe how excision are grossed with orientation and without.
8. Potential grossing errors, how to prevent them.
9. Demonstrate and exhibit positive professional attitudes at all time in the laboratory, including confidentially and ethical decision-making. HIPPA
10. Demonstrate knowledge of the principles of laboratory safety.
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CE credits: Total CE credit (1) Credits, 1 CE Histology Delivery method: Live, classroom
Course hours: 1 hours. Content: 10 pages Exam Information:
• 10 questions • Passing score
Content Outline
1. How to set up, operate and maintain instruments for gross room station.
2.0 List of instruments and supplies for room station.
3.0 Orientation of tissues and inking of specimens for macroscopically and microscopic orientation.
4.0 Fixation and handling of dermatological specimens.
5.0 Types of specimens receive in the dermatology laboratory.
6.0 Describe grossing protocols.
c. grossing protocol for excisions.
7.0 Excision are grossed with orientation and without.
8.0 Grossing potential error.
EMBEDDING IN PARAFFIN, DERMATOLOGICAL SPECIMENS. PART IV. CE TRACKER# 20-728828
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/10/2019
Description:
This course examines the proper embedding of standardized techniques for skin specimens to obtain optimal diagnostic slides from skin. It is known that the orientation of skin tissue in embedding is determined by the epidermis. The proper orientation has the most impact on the diagnostic, if the lesion area is cut away, a diagnosis is not possible. That is one of the reasons that some Histotechnician consider the skin embedding a challenge.
Objectives:
1. Summarize histology of skin, layers. 2. Outline general rules of tissue embedding. 3. Describe skin embedding techniques. 4. Recognize embedding artifact and how troubleshoot them. 5. List alternative embedding media.
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
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a. Epidermis
b. Dermis
a. Embedding in paraffin definition.
b. General embedding of a tissue in paraffin
c. Choosing the correct paraffin, mold and cassettes
d. Types of orientation a tissue of different organs.
3. Skin Embedding protocols.
4. Artifacts in the embedding process and how to avoid them.
5. Alternative embedding media.
CUTTING SECTIONS IN PARAFFIN, DERMATOLOGICAL SPECIMENS. PART V. CE TRACKER# 20- 728834.
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/10/2019
Description:
This course examines the proper cutting techniques for skin specimens to obtain optimal diagnostic slides from skin. It is known that the orientation and cutting sections has the most impact on the diagnostic, if the lesion area is cut away, a diagnosis is not possible. That is one of the reasons that some Histotechnician consider the skin cutting a challenge.
Objectives:
1. Summarize histology of skin, layers. 2. Outline general rules of tissue sectioning in paraffin. 3. Describe skin sectioning techniques. 4. Recognize sectioning artifacts and how troubleshoot them. 5. Describe other alternative cutting methods.
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CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
Content Outline:
a. Epidermis
b. Dermis
a. Choosing the correct knives.
d. Orientation of the skin during embedding.
3. Skin sectioning protocols.
4. Artifacts in the sectioning process and how to avoid them.
5. Alternative cutting sections.
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HAIR HISTOLOGY, AND INSTRUCTIONS FOR SCALP PUNCH BIOPSIES. PART VI. CE TRACKER# 20-728834
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/10/2019
Description:
Getting a proper scalp biopsy and following the stablished protocol steps during accessioning, grossing and embedding are important elements in diagnosing a patient with hair loss.
This course is oriented to review the histological structure of the hair, the importance of the diagnosis and care of technical staff of the request of the physician about the test to be done, which must be noted by technical personnel during accessioning, the surgical grossing techniques and embedding protocols of scalp punch biopsies. Recommendations for cutting sections of transversal punch biopsies are reviewed.
Objectives:
This course is oriented to review the histological structure of the hair, accessioning, embedding protocol for general skin punch biopsies and scalp biopsies. Recommendations of transverse cut sections of scalp hairs.
Intended audience:
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
Course hours: 1 hours. Content: 10 pages Content Outline: Content outline: 1.0. Anatomy of the hair and hair follicle 1.1. Origin of the hair 1.2. Histological Characteristic of the different layers of the hair follicle. 1.3. Hair follicles cycles of hair growth. 2.0. Describe histology of sebaceous gland. 3.0. Describe histology of sweat glands. 4.0. Accessioning, fixation of punch biopsy.
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4.1. Grossing protocols of punch biopsies of skin and scalp biopsy 4.2. Embedding protocols on punch biopsies of skin and scalp biopsy. 4.3. Cutting protocols of skin punch biopsies and scalp. NAIL HISTOLOGICAL STRUCTURE, ACCESSIONING, PROCESSING, CUTTING, AND STAINS FOR HISTOPATHOLOGY STUDIES, PART VII. CE TRACKER# 20-729012
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/10/2019
Description:
Nail disorders can arise at any age. About half of all nail disorders are of infectious origin, 15% are due to inflammatory or metabolic conditions, and 5% are due to malignancies and pigment disturbances. The differential diagnosis of nail disorders is an area of interest of histopathology. Malignant tumors of the nails are often not correctly diagnosed at first. For subungual melanoma, the mean time from the initial symptom to the correct diagnosis is approximately 2 years; this delay can be partly responsible for the low 10-year survival rate of only 43%. A correct histological evaluation of the nail tissue is an important diagnostic instrument. This course is designated to aim to improve the knowledge and skills of the technical personnel trough all steps of the nail histology procedure in the histopathology laboratory.
Objectives:
1.0. Describe the anatomy of the nail. 1.1. Summarize histological characteristic of nails. 2.0. Describe areas of pathological interest. 3.0. Accessioning and fixation of nail 3.1. Recommendations to avoid falling off the nail clipping. 4.1. List common regular and special stains used for nail histopathological studies.
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
Content Outline:
1.0. Anatomy of the nail. Parts 1.1. Normal histology of nails. 2.0. Areas of pathological interest in nails. Common pathologies. 3.0. Accessioning and fixation of nail recommendations 3.1. Recommendations to avoid falling off the nail clipping. 3.2. Recommendations for cutting nail embedded in paraffin. 4.1. List common regular and special stains used for nail histopathological studies.
DERMATOLOGICAL SPECIMENS, USEFUL SPECIAL STAINS USED IN THEIR DIAGNOSTIC. PART VIII.
CE TRACKER #: 20-729078 Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/12/2019 Description:
Diagnostic evaluation of disorders of skin tissue is largely based on a thorough examination of the sections stained with hematoxylin and eosin (H&E). Additional special stains may be used to highlight or identify features that are not easily seen on an H&E stain. The choice of stain or panel of stains depends on the findings on initial assessment, the clinical context, and the preference of the pathologist. Every patient may require several special stains for an optimal assessment. The intent of this course is to discuss the common stains used in the evaluation of dermatological specimens.
There are a lot of special stains available in the histopathology laboratory that can be used for the detection of microorganisms y/ or cellular components. Due to complexity of some of the special stain procedures and other factors, the quality of stain sometimes can be poor and no suitable tor diagnosis, this failure can be solved in dependence of the skills of the technical personnel. Some more advances laboratories have special staining automatic machines, making easier the task and reducing the TAT of the laboratory. However, all technical personnel must seriously consider having the manual skills of perform any special stain needed in the laboratory, this complete the qualification of the personnel. This participation in this course will be of aim to complete and update the knowledge and skills necessary in this important area of work.
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Objectives:
1.0. Understand staining methods used in the clinical histopathology for skin pathologies. 2.0. List special stains used in histopathological studies of skin tissue. 3.0. Describe purpose, methods and procedures of each of them. 4.0. Discuss more common factors than can affect the quality of special stain results and how troubleshoot them. 5.0. Demonstrate skills to follow a stain protocol and prepare working solutions.
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
• 10 questions • Passing score
Content Outline
1.0 Components of skin tissue and staining methods used in histopathology of skin
2.0 Special stains used in skin tissue studies.
2.1 CONECTIVE TISSUE: COLLAGEN; PTHA – Mallory, Masson Trichrome, Movat, Picric Sirious, H Gomori one-step trichrome, Van Gieson ELASTIC FIBERS: Verhoeff RETICULIN; Gordon&Sweet, PTHA MUCOPOLISACHARIDES: Alcian Blue, colloidal Iron. AMYLOID: Crystal Violet, Congo Red BACTERIA: B&B, AFB Ziehl-Nelsen, Fite -Faraco, Steiner, Mucicarmin FUNGUS: PAS, GMS, PIGMENTS: melanin, Fontana-Masson MINERALS: Iron, Von Kossa-Calcium.
3.0 Purpose, methods and procedures of each of stains listed above.
4.0 Factors that affect results and quality of special stains and how troubleshooting 4.1 Troubleshooting
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OVERVIEW OF IMMUNOHISTOCHEMISTRY (IHC) OF THE SKIN.
Part IX CE TRAKER #20-729286. Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/15/2019
Authors: Carmen I. Perez. HTL, BSc accredited by FIU, M.D Maritza R. Martinez. HTL, BSc accredited by FIU, Ph.D. Medical Science. Created: 02/1/2019 Updated: 7/12/2019
Description:
Diagnostic evaluation of disorders of skin tissue is largely based on a thorough examination of sections stained with hematoxylin and eosin (H&E). Additional IHC stains are used by the pathologist to highlight or identify features that are not easily interpret on an H&E stain. The choice of IHC stain or panel of stains depends on the findings on initial assessment, the clinical context, and the preference of the pathologist. Every patient may require several IHC stains for an optimal assessment. Some more advances laboratories have IHC automatic machines, making easier the task and reducing the TAT of the laboratory. However, all technical personnel must seriously consider having the skills to perform any IHC stain needed in the laboratory, this complete the qualification of the technical personnel. The participation in this course will be of aim to complete the knowledge and skills necessary in this important area of work.
The intent of this course is to discuss IHC stains used in the evaluation of dermatopathology specimens.
Objectives:
1.0. Understand staining methods used in the clinical histopathology for skin pathologies. 2.0. Explain factors that affect results and quality of IHC stain and how troubleshooting them. 3.0 List IHC stains used in skin tissue 4.0 Discuss more common factors than can affect the quality of IHC stain results.
CE credits: Total CE credit (1) Credits, 1 CE Histology
Delivery method: Live, classroom
Course hours: 1 hours.
• 10 questions • Passing score
Content Outline:
1.0. Staining methods used in the clinical histopathology for skin pathologies.
2.0. Factors that affect results and quality of IHC stain and how troubleshooting them.
3.0. IHC stains panels used in skin pathologies.
4.0. Common factors than can affect the quality of IHC stain results.
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