Si Nitride Chip.
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Transcript of Si Nitride Chip.
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Si Nitride Chip.
4.4 mm
4.4 mm Window
unmodified silicon nitride window/chip as supplied by Adam Hall (Norcada NX4402x)window is 30nm nitride, it is 10-20um (these look like 20um) square in a frame of 4.4mmx4.4mm 200um thick Si
http://www.norcada.com/nitride_window_xray.php - all image areas were intended to overlap or fall closely adjacent to window
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samples returned by Adam Hall - pores and origami (AH1-4) or just pores (AH5a and 5b)AH1 5x5, 400 nm sep, origami sample 1, measured in 1 M KCl w/ TE and MgCl2 (this is
the source of the sample trace and of the one AFM image I sent)AH2 5x5, 400 nm sep, origami sample 2, measured in 50 mM KCl w/ TE and MgCl2AH3 5x5, ~2.5 um sep, origami sample 1, measured in 1 M KCl w/ TE and MgCl2AH4 5x5, ~1.6 um sep, origami sample 1, measured in 1 M KCl w/ TE and MgCl2AH5a no origami 400nm sepAH5b no origami 2.8um sep
CO samples from Marshall - origami sample 1-4 Sample 1 contains 1.7 nM origami (12ng/ul) with no extra tail extender
Sample 2 contains 1.7 nM origami (12ng/ul) plus 200nm tail extension at about 1:1 ratio
Sample 3 contains 1.7 nM origami (12ng/ul) plus 1200nm tail extension at about 1:1 ratio with origami
Sample 4 contains ~10nM origami with 5x extra staples - this is unpurified so should have longest shelf life
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Adam Hall (AH) Samples 1, 2, and 5a. 400-nm nanopore spacing.
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Adam Hall (AH) Sample 4. 1.6-µm nanopore spacing.
20 µm
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Adam Hall (AH) Samples 4 1600-nm nanopore spacing.
AH4 - unrinsed, as recieved
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Adam Hall (AH) Samples 4 1600-nm nanopore spacing.
AH4 -imaged on crystal - then Tanner rinsed surface -looked clean no more crystals - to be continued
approx., location of new zoomed scan below
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Adam Hall (AH) Samples 4 1600-nm nanopore spacing.
AH4 -imaged on crystal - then Tanner rinsed surface -looked clean no more crystals – images follow
AH4 - off window
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Adam Hall (AH) Samples 4 1600-nm nanopore spacing.
AH4 - rinsed
AH4 - off window
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Adam Hall (AH) Samples 4 1600-nm nanopore spacing.
AH4 -rinsed
AH4 - at window
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Adam Hall (AH) Samples 4 1600-nm nanopore spacing.
AH4 rinsed
AH4 - on window – no array visible
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Adam Hall (AH) Samples 4 1600-nm nanopore spacing.
AH4 -rinsed
AH4 - on window - tried for dead center of window
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