Shop! - 178.250.165.133178.250.165.133/ex/downloads/life_science/biomarke/0811/... ·...

4
Promotions for Life Scientists ISSUE 12 - NOVEMBER 2008 ValId UNtIl 31St MaRch 2009 chEcK OUt WWW.VWR.cOM FOR FUll SPEcIFIcatIONS http://dk.vwr.com Local price agreements apply. Shop! ISSUE 12 NOVEMBER 2008 IN THIS ISSUE DK WWW.VWR.COM Editor VWR International Europe bvba Haasrode Researchpark Zone 3 Geldenaaksebaan 464 3001 Leuven Belgium Copywriting VWR International Layout and typesetting Marketing Services VWR Printing Stork, Germany No part of this publication may be reproduced or copied without prior permission by writing of VWR International Europe. Run 80.500 copies Publication date: November 2008 Due to the high sales volume of promoted articles some items may be temporarily out of stock - VWR Terms and Conditions of Sale apply. CapitalBio LuxScan 10K – A dual channel confocal microarray scanner - that you CAN afford! A compact high performance scanner system for microarray imaging and data analysis of DNA, protein and cell arrays. Integrating many advanced optical, signal processing and motion control systems, the LuxScan 10K has two excitation lasers and provides high detection sensitivity, accuracy, dynamic range and linearity. Advanced and optimised signal acquisition and processing elements, make it an ideal tool for profiling microarray-based gene expression assays. Laser intensity and PMT are continuously adjustable Superior image quality, Signal to Noise Ratio exceeds other equivalent products Free software upgrades for first 5 years To claim simply email the serial number of the unit to [email protected] with your choice Up for DOPPIO PCR? The VWR Collection DOPPIO thermal cycler is an ideal way to maximise research funds by investing in a really flexible cycler. Two independent 48 well blocks, both with sprung heated lids will adapt to both tubes and high profile 48 well plates. Two programs can be run independently or one program simultaneously on both blocks. Either way, the DOPPIO provides two-block functionality at an incredible price. Want gradients? Who wouldn’t?! The DOPPIO can run gradient programs on both or either thermal blocks as a standard feature. Simply enter a gradient step into your protocol, and the DOPPIO will generate a gradient over 6 columns. For a wider gradient range, then simply use both blocks. As part of the VWR Collection thermal cycler family, the DOPPIO benefits from the same software architecture as the other products in the range, using Windows CE as its control platform, with a 3,5” full colour touch screen. Users are able to store up to 1000 programs within the onboard software. Molecular Biology promo code 1 Special intro price and 1.500 DKK of free oligos promo code 2 Molecular Biology New DOPPIO thermal cycler from the VWR Collection CapitalBio LuxScan microarray scanner New KOD DNA Polymerases from Merck’s Novagen ® range Omega Biotek E.Z.N.A large scale plasmid preparation kits Thermo Scientific barcoded 96 well PCR plates PCR Mycoplasma Test Kit Three clicks away from pure DNA with Mole! Easy to use custom oligonucleotide online order tool! Electrophoresis It’s time for 5 PRIME’s perfect PCR partners Precast gels for protein electrophoresis from Lonza CBS Scientific adjustable slab gel kit Cell biology AutovaxID cell culture instrument increases mAb concentration by 50% Efficient Fusion with BTX BRANDplates - discover the broad range of the new microplates for immunological applications! Ultimate low cell binding cultureware from Nunc BD Primaria cell culture products NALGENE ® high speed centrifuge tubes and bottles Wheaton 2 ml cryogenic sterile Cryule ® vials VWR Collection incubating orbital shaker Sample prep Simple, reliable concentrating and desalting with Pall NanoSep devices Ready-to-use dialysis device from Spectrum Microplate reading Enhance your kinetic assays with the Thermo Scientific microplate fluorometers and luminometers! Chip Format (mm) 25 × 75 Scan Resolution (μm) User selectable: 5, 10, 20, 40 Sensitivity 0,1 fluorescent molecules/ μm 2 Scan Speed 8 mins / chip (10 μm resolution) Dynamic Range 10 4 Repeatability 95% Laser Power Settings 1-100% continuously adjustable PMT Gain 450-950 continuously adjustable Maximum Scan Area (mm) 22 × 72 Focus Search Range (μm) ± 250 Excitation Wavelength (nm) 532 and / or 635 Applicable Fluorescence Dyes Cy3, Cy5, etc. Emission Wavelength 570 ± 10 nm, 675 ± 10 nm (expandable to 6 types) Image File Format TIFF, BMP, JPG, RAW Size (W × D × H) (mm) 360 × 540 × 220 Weight (kg) 40 E.Z.N.A. ® large scale plasmid preparation kits Takes less than 40 minutes High quality plasmid suitable for most downstream applications Low endotoxin levels for Plasmid Mega and Giga Preps Vacuum protocol saves 20 minutes over centrifugation protocol for Midi and Maxi Preps *Limit one per customer ** Includes free vacuum manifold (Vac-08P) Order a large scale plasmid kit and get a vacuum manifold free* promo code 4 Great price and over DKK 5.500 of free accessories! New KOD DNA Polymerases from Merck’s Novagen ® range KOD Xtreme Hot Start DNA Polymerase Amplification of difficult DNA targets Amplifies DNA with up to 90% GC-content Amplifies targets up to 40 kbp Optimised for the highest success rate KOD Hot Start Master Mix - Our top performing PCR Polymerase now as Master Mix! Highest accuracy, yield and processivity Amplifies templates up to 21 kbp Eliminates mispriming and primer-dimer formation KOD Hot Start Buffer ensures optimal PCR performance More information in Novagen’s inNovations Newsletter 28 – order your free copy from your local VWR office. promo code 3 NEW! When ordering KOD products listed below you will receive a free 2009 Interactive Pathways Calendar. Order your DOPPIO now, and take advantage of the introductory offer. Email your machine’s serial number to [email protected] and we will send you a free of charge voucher from Thermo Scientific Biopolymers on oligos to a value of 1.500 DKK. Additional DKK 5.500 in FREE consumables including 2 hybridisation cassettes and 4 packs of 25 blank microarray slides. Choose the appropriate slide coating, amino or aldehyde modified, ideal for spotting PCR products and oligonucleotides; or epoxy or polymer modified slides, ideal for proteins. Description Cat. No. Price Introductory price DOPPIO Thermal Cycler 732-1210P 23500,- 20000,- Description Size (mm) Cat. No. Price Promo price LuxScan 10K Microarray Scanner 360 × 540 × 220 737-0152P 313350,- 193500,- Micro array slides Qty/Pk Cat. No. Price Amino coating 25 737-0097P 2400,- Aldehyde coating 25 737-0101P 2620,- Epoxy coating 25 737-0105P 2470,- Polymer coating 25 737-0109P 2010,- Cassette hybridisation gene chip 1 737-0112P 958,- Kits Preps Cat. No. Price** Plasmid Midiprep 100 OMEGD6904-04P 2970,- Plasmid Maxiprep 100 OMEGD6922-04P 6010,- Fastfilter Midiprep 100 OMEGD6905-04P 3710,- Fastfilter Maxiprep 100 OMEGD6924-04P 6450,- Fastfilter Mega prep 20 OMEGD6929-03P 5180,- Fastfilter Gigaprep 20 OMEGD6920-03P 7260,- Product Size Cat. No. Price KOD Xtreme Hot Start DNA Polymerase 200 U 71975-3P 4740,- KOD Hot Start Master Mix 100 rxn 71842-3P 1580,- KOD Hot Start Master Mix 500 rxn 71842-4P 6320,- BioMarkeShop_12_DK.indd 1 10/10/2008 11:26:03 AM

Transcript of Shop! - 178.250.165.133178.250.165.133/ex/downloads/life_science/biomarke/0811/... ·...

Page 1: Shop! - 178.250.165.133178.250.165.133/ex/downloads/life_science/biomarke/0811/... · electrophoresis • It’s time for ... 2 Promotions in the VWrbioMarke SHOP may not be used

Promotions for Life Scientists

ISSUE 12 - NOVEMBER 2008 • ValId UNtIl 31St MaRch 2009 • chEcK OUt WWW.VWR.cOM FOR FUll SPEcIFIcatIONS

http://dk.vwr.com

Local price agreements apply.

Shop!ISSUE 12 NOVEMbEr 2008in this issue

DK

www.vwr.comeditorVWr International Europe bvbaHaasrode researchpark Zone 3Geldenaaksebaan 4643001 Leuvenbelgium

copywritingVWr International

Layout and typesettingMarketing Services VWr

PrintingStork, Germany

No part of this publication may be reproduced or copied without prior permission by writing of VWr International Europe.

run80.500 copiesPublication date: November 2008

Due to the high sales volume of promoted articles someitems may be temporarily out of stock - VWr Terms andConditions of Sale apply.

capitalBio Luxscan™ 10K – A dual channel confocal microarray scanner - that you cAn afford!

A compact high performance scanner system for microarray imaging and data analysis of DNA, protein and cell arrays. Integrating many advanced optical, signal processing and motion control systems, the LuxScan™

10K has two excitation lasers and provides high detection sensitivity, accuracy, dynamic range and linearity. Advanced and optimised signal acquisition and processing elements, make it an ideal tool for profiling microarray-based gene expression assays.

Laser intensity and PMT are continuously adjustable•Superior image quality, Signal to Noise ratio •exceeds other equivalent productsFree software upgrades for first 5 years•

To claim simply email the serial number of the unit to [email protected] with your choice

up for DoPPio Pcr?The VWr Collection DOPPIO thermal cycler is an ideal way to maximise research funds by investing in a really flexible cycler. Two independent 48 well blocks, both with sprung heated lids will adapt to both tubes and high profile 48 well plates. Two programs can be run independently or one program simultaneously on both blocks. Either way, the DOPPIO provides two-block functionality at an incredible price.

Want gradients? Who wouldn’t?! The DOPPIO can run gradient programs on both or either thermal blocks as a standard feature. Simply enter a gradient step into your protocol, and the DOPPIO will generate a gradient over 6 columns. For a wider gradient range, then simply use both blocks.

As part of the VWr Collection thermal cycler family, the DOPPIO benefits from the same software architecture as the other products in the range, using Windows CE as its control platform, with a 3,5” full colour touch screen. Users are able to store up to 1000 programs within the onboard software.

molecular Biologypromo

code1

special intro price and 1.500 DKK of free oligos

promo code

2

molecular BiologyNew DOPPIO thermal cycler from the •VWr Collection

Capitalbio LuxScan• ™ microarray scannerNew KOD DNA Polymerases from •Merck’s Novagen® rangeOmega biotek E.Z.N.A large scale plasmid •preparation kitsThermo Scientific barcoded 96 well PCr •platesPCr Mycoplasma Test Kit•Three clicks away from pure DNA with •Mole!Easy to use custom oligonucleotide •online order tool!

electrophoresisIt’s time for 5 PrIME’s perfect PCr •partnersPrecast gels for protein electrophoresis •from LonzaCbS Scientific adjustable slab gel kit •

cell biologyAutovaxID• ™ cell culture instrument increases mAb concentration by 50%Efficient Fusion with bTX•brANDplates - discover the broad •range of the new microplates for immunological applications!Ultimate low cell binding cultureware •from NuncbD Primaria• ™ cell culture products NALGENE• ® high speed centrifuge tubes and bottlesWheaton 2 ml cryogenic sterile Cryule• ® vialsVWr Collection incubating orbital •shaker

sample prepSimple, reliable concentrating and •desalting with Pall NanoSep devicesready-to-use dialysis device from •Spectrum

microplate readingEnhance your kinetic assays with •the Thermo Scientific microplate fluorometers and luminometers! Chip Format (mm) 25 × 75

ScanResolution(μm) User selectable: 5, 10, 20, 40

Sensitivity 0,1fluorescentmolecules/μm2

Scan Speed 8mins/chip(10μmresolution)

Dynamic range 104

repeatability 95%

Laser Power Settings 1-100% continuously adjustable

PMT Gain 450-950 continuously adjustable

Maximum Scan Area (mm) 22 × 72

FocusSearchRange(μm) ± 250

Excitation Wavelength (nm) 532 and / or 635

Applicable Fluorescence Dyes Cy3, Cy5, etc.

Emission Wavelength 570 ± 10 nm, 675 ± 10 nm (expandable to 6 types)

Image File Format TIFF, bMP, JPG, rAW

Size (W × D × H) (mm) 360 × 540 × 220

Weight (kg) 40

e.Z.n.A.® large scale plasmid preparation kits

Takes less than 40 minutes•High quality plasmid suitable •for most downstream applicationsLow endotoxin levels for •Plasmid Mega and Giga PrepsVacuum protocol saves 20 •minutes over centrifugation protocol for Midi and Maxi Preps

*Limit one per customer ** Includes free vacuum manifold (Vac-08P)

order a large scale plasmid kit and get a vacuum

manifold free*

promo code

4

Great price and over DKK 5.500 of free accessories!

new KoD DnA Polymerases from merck’s novagen® rangeKoD Xtreme™ hot start DnA Polymerase

Amplification of difficult DNA targets•Amplifies DNA with up to 90% GC-content •Amplifies targets up to 40 kbp •Optimised for the highest success rate•

KoD hot start master mix - our top performing Pcr Polymerase now as master mix!

Highest accuracy, yield and processivity •Amplifies templates up to 21 kbp •Eliminates mispriming and primer-dimer formation •KOD Hot Start buffer ensures optimal PCr •performance

more information in novagen’s innovations newsletter 28 – order your free copy from your local VWR office.

promo code

3

NEW!

when ordering KoD products listed below you will receive a free 2009 interactive

Pathways calendar.

order your DoPPio now, and take advantage of the

introductory offer. email your machine’s serial number to [email protected]

and we will send you a free of charge voucher from thermo

scientific Biopolymers on oligos to a value of 1.500 DKK.

Additional DKK 5.500 in FrEE consumables including 2 hybridisation cassettes and 4 packs of 25 blank microarray slides. Choose the appropriate slide coating, amino or aldehyde modified, ideal for spotting PCr products and oligonucleotides; or epoxy or polymer modified slides, ideal for proteins.

Description cat. no. Price introductory priceDOPPIO Thermal Cycler 732-1210P 23500,- 20000,-

Description size (mm) cat. no. Price Promo priceLuxScan™ 10K Microarray Scanner 360 × 540 × 220 737-0152P 313350,- 193500,-

micro array slides Qty/Pk cat. no. PriceAmino coating 25 737-0097P 2400,-

Aldehyde coating 25 737-0101P 2620,-

Epoxy coating 25 737-0105P 2470,-

Polymer coating 25 737-0109P 2010,-

Cassette hybridisation gene chip 1 737-0112P 958,-

Kits Preps cat. no. Price**Plasmid Midiprep 100 OMEGD6904-04P 2970,-

Plasmid Maxiprep 100 OMEGD6922-04P 6010,-

Fastfilter Midiprep 100 OMEGD6905-04P 3710,-

Fastfilter Maxiprep 100 OMEGD6924-04P 6450,-

Fastfilter Mega prep 20 OMEGD6929-03P 5180,-

Fastfilter Gigaprep 20 OMEGD6920-03P 7260,-

Product size cat. no. PriceKOD Xtreme™ Hot Start DNA Polymerase 200 U 71975-3P 4740,-

KOD Hot Start Master Mix 100 rxn 71842-3P 1580,-

KOD Hot Start Master Mix 500 rxn 71842-4P 6320,-

BioMarkeShop_12_DK.indd 1 10/10/2008 11:26:03 AM

Page 2: Shop! - 178.250.165.133178.250.165.133/ex/downloads/life_science/biomarke/0811/... · electrophoresis • It’s time for ... 2 Promotions in the VWrbioMarke SHOP may not be used

Place your order today! visit our website http://dk.vwr.comLocal price agreements apply.

offers valid from 1st november 2008 to 31st march 2009Promotions in the VWrbioMarke SHOP may not be used in combination, VWr reserves the right to refuse invalid orders.2

ISSUE 12 - NOVEMbEr 2008Shop!

AutovaxiD™ cell culture instrument increases mAb concentration by 50%AutovaxID™ is the next generation in automated cell culture production systems. All the instrumentation necessary for cell culture production is enclosed within a footprint of less than 0,3 m2. The simplicity and software automation allows the user to focus on cell production. Users go from box to operation in less than 5 minutes and can be fully efficient in just one day!! Product concentration in the AutovaxID™ is 10X to 30X higher than conventional cell culture methods. The unique two component design reduces the need for a clean room, as well as reduces the risk of process contamination.

reduces time by 80%•Takes only 20% of the space•reduced cost per mg of mAb by 60%•replaces 80 l stirred tank•

promo code

11

Pcr mycoplasma test Kit The PCr Mycoplasma test kit is designed to detect the presence of mycoplasma contaminating biological materials, in for example cultured cells. Whereas mycoplasma detection by culturing can be difficult and time consuming, PCr testing gives results in a few hours.

Contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis. There is no need to prepare probes labelled with radioisotopes, or to calculate enzyme, dNTP’s or buffer concentrations. Instead, a ready to use, optimised PCr mix is supplied.

The primer set allows detection of various mycoplasma species (M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum and M. capricolum), as well as Acholeplasma and Spiroplasma species, with high sensitivity and specificity.

Simple, one-step reaction; less than 10 minutes to prepare the sample•Can be used routinely in every lab•Minimal sample handling reducing the risk of contamination•Sensitive (100 – 1000 CFU/ml)•M. hyorhinis• can be detected

promo code

6

it’s time for 5 Prime’s perfect Pcr partnersthe easy way to your Pcr fragments – self adjusting Pcr buffer and accurate DnA fragment sizing.

combine the ultra-pure, thermostable recombinant 5 Prime Taq Polymerase with the new Perfectsize DnA molecular weight Ladders for optimal Pcr results.

5 PrIME Taq Polymerase enables you to get fragments from even the most specialised PCr applications while 5 PrIME PerfectSize DNA Molecular Weight Ladders allow accurate size determination and quantification of DNA fragments during electrophoresis. 5 PrIME Taq Polymerase features the self-adjusting Mg2+ buffer technology that automatically adjusts the Mg2+ concentration. This provides optimal conditions for any primer-template system, saving time and precious samples.

Five different 5 PrIME PerfectSize DNA Molecular Weight Ladders cover a wide range of fragment sizes at regular intervals and defined fragment concentrations. Each 600 µl vial is sufficient for 100 applications and is ready to load with a buffer containing three tracking dyes for optimal control of agarose gel run times.

electrophoresis

Cat. No. 733-0180P, Taq DNA Polymerase – 1000 UAmplification of fragments with different lengths using Taq DNA Polymerase.1. 160 bp fragment of apolipoprotein b (genomic DNA)2. 360 bp fragment of apolipoprotein b (genomic DNA)3. 400 bp fragment of Taq DNA Polymerase gene (60% GC, plasmid DNA)4. 547 bp fragment, GC rich (70% GC, genomic DNA)5. 10 kb fragment from the genome of lambda phage6. DNA Marker

Cat. No. 733-1794P PerfectSize 100 bp XL Ladder – 100 Produces eleven sharp bands from 100 to 1500 bp. Analysis on a 2.0% agarose gel.

three clicks away from pure DnA! DnA as simple as making coffee!

DNA extraction can’t get any simpler. GeneMole® is the small scale automated DNA extractor for every research lab. It is intuitive to operate and requires minimal preparation. Anyone can use it without training or special skills. best of all this system does not ruin your budget! Share your GeneMole® with colleagues or have one just for yourself.

promo code

7

Adjustable slab gel kit16,5 cm wide and adjustable to a 16, 17, 19, 22 or 28 cm path length, this unit features adjustable upper reservoir, levelling base, platinum electrodes and safety-tip power leads. Includes 2 each 1,5 mm x 20 well combs, 2 each Gel Wrap™ gaskets, 2 sets of 22 cm glass plates, 2 sets of 1,5 mm thick spacers, 8 white spring clamps.

promo code

8

promo code

10

*Incubator clean solution 500ml Cat. No. A5230.0500

track it! Barcoded 96 well Pcr platesAccurate tracking of both samples and data is essential in PCr/QPCr. To help you easily track and locate your samples, Abgene have extended their product range to include a barcoded 96 well PCr plate that fits the AbI fast block PCr and QPCr machines.

The thin walled design ensures that optimal and consistent heat transfer occurs, giving consistent high quality results. The barcode is a standard code 128 and is positioned on the opposite alpha side of the plate in the centre - the standard reading position for any of the AbI platforms.

Guaranteed to give you a perfect fit and like all Abgene plastics free from any contaminants such as DNA, rNA, DNase and rNase.

Code 128 barcoding is available on any skirted PCr plate, please enquire for further details. Sorry, not available in Italy, belgium and Netherlands

Buy 2 packs pay for only 1!

promo code

5

The mixture contains everything (incl. Taq Pol.)

PAGer® Precast Gels for Protein electrophoresiseasy-to-load, easy-to-open, easy-to-try

PAGEr® Gels are easy-to-use protein minigels that offer sharper resolution, more consistent protein transfer, and longer shelf life than any other Tris-Glycine gel. PAGEr® Gels are easy to use and compatible with most minigel chambers.

See clearly for loading with our marked sample lanes •and tinted cassettesSnap open the cassettes with a simple twist of the •combrun PAGEr• ® Gels in your existing chamber

promo code

9

The AutovaxID™ is 3 times more economical than traditional roller bottle or stirred tank methodsKey benefits:

Flexibility to choose from •1 to 16 samples/runJust three minutes handson time•Convenient prefilled and sealed reagent strips•Occupies minimal bench space•

* To claim simply email the serial number of the unit to [email protected] with your choice of reagents

order 5 Prime Taq and get a free vial of the Perfectsize 100bp XL ladder

Prosieve® colour Protein marker Free with the

purchase of any box of PAGer® Gels

Free 500 ml incubator clean solution *

For a full list of products or a free sample contact your local vwr sales office.

extra comb, spacer set, Gel wrap™ gasket and glass plate set Free.

Fantastic price and DKK 7.500 of free

reagents with every Genemole® instrument

purchase*

Description cat. no. Price PCr Mycoplasma Test kit A3744.0020P 3090,-

Description range Bands content cat. no. PriceTaq DNA Polymerase 1000 U 733-0180P 1981,-

PerfectSize 50bp Ladder 50 – 500 bp 9 600 µl 733-1792P 1040,-

PerfectSize 100bp Ladder 100 – 600bp 6 600 µl 733-1793P 925,-

PerfectSize 100bp XL Ladder 100 – 1500bp 11 600 µl 733-1794P 1040,-

PerfectSize 1kb Ladder 1kb – 10kb 9 600 µl 733-1795P 578,-

PerfectSize 1kb XL Ladder 100bp – 10kb 12 600 µl 733-1796P 694,-

Description Qty/Pk cat. no. Price GeneMole® instrument* 1 731-3051P 59990,-

MoleStrips™ DNA blood (test-kit) 32 731-3050P 1230,-

MoleStrips™ DNA blood 64 731-3053P 2160,-

MoleStrips™ DNA Tissue (test-kit) 32 731-3052P 1470,-

MoleStrips™ DNA Tissue 64 731-3054P 2550,-

Description cat. no. PriceAdjustable slab gel promo kit 730-1816P 5320,-

Description Qty/Pk cat. no. Price96 well fast block PCr plate with barcode 128 25 731-0136P 7980,-

96 well fast block PCr plate with barcode 128, white 25 731-0137P 8700,-

For more information contact your local vwr sales office.

BioMarkeShop_12_DK.indd 2 10/10/2008 11:26:18 AM

Page 3: Shop! - 178.250.165.133178.250.165.133/ex/downloads/life_science/biomarke/0811/... · electrophoresis • It’s time for ... 2 Promotions in the VWrbioMarke SHOP may not be used

Place your order today! visit our website http://dk.vwr.comLocal price agreements apply.

offers valid from 1st november 2008 to 31st march 2009Promotions in the VWrbioMarke SHOP may not be used in combination, VWr reserves the right to refuse invalid orders. 3

ISSUE 12 - NOVEMbEr 2008Shop!efficient fusion with BtXElectrofusion is a highly efficient, non-toxic technique suitable for nuclear transfer cloning, embryonic fusion, fusion or transfections of mammalian cells and plant protoplasts to name but a few applications. The ECM 2001 is a versatile system for both Electrofusion and Electroporation. Fusion can be viewed or manipulated under a microscope with the bTX Microslide chambers.

Incorporates both AC and DC (square) waveforms•Compatible with specialty electrodes for • in vivo applications

promo code

12

cell Biology

Ac Parameters (Alignment) nonsinusoidal wave:

Frequency 1 MHz

Voltage 0 – 75 V (zero to peak)

Duration 0 – 99 sec

Post fusion AC 1/10 of pre-fusion amplitude

Post fusion ramp 1 – 9 sec

Pause between AC/DC 50μsec

Dc Pulse Parameters (Fusion) (electroporation) square wave:

High Voltage Mode:

Voltage (V) 10 – 3000

Pulse Length 1–99μsec

Low Voltage Mode:

Voltage (V) 10 – 500

Pulse Length 0,01 – 0,99 msec

resolution 0,01 msec

Number of Pulses 1 – 9

ultimate low cell binding culturewarenunc hydrocell™ Plates and Dishes for single-cell and cell-cluster suspension cultures.

The HydroCell product range consists of MicroWell plates, MultiDishes and Dishes with a super-hydrophilic polymer bound covalently to the culture surface, allowing suspension cultivation of single cells and cell clusters that otherwise adhere to tissue-culture treated and non-treated conventional cultureware.

promo code

14

Examples of HydroCell applications: 1) Cultivation and analysis of macrophages and other monocyte-derived cell types; 2) Stem cell propagation (for example, neurospheres); 3) Stem cell differentiation (for example, embryonic stem cells to embryoid bodies); 4) Cultivation and analysis of tumor-cell spheroids; and 5) Analysis of cell secretions with minimal loss of sample to surface adsorption).

Licensed from CellSeed Inc. * Maximum one box of each product per customer

Discover the broad range of the new microplates from BrAnD for immunological applicationsModern research methods require high-quality disposables. brANDplates®, the new generation of 132 microplates from brAND can be used in a broad range of applications such as ELISA, rIA, FIA, etc. Three new immunological surfaces have been developed under the most modern production conditions.

immuno-treated (non-sterile)Depending on the characteristics of the molecules to be attached to the surface choose between these different types of polystyrene brANDplates® for solid phase assays:

immunoGrade™: Optimised for immobilisation of IgG (hydrophilic and hydrophobic regions), comparable to “high-binding” plates from other manufacturers

hydroGrade™: More hydrophilic than immunoGrade™, for the attachment of hydrophilic molecules like components of the complement system or glycoproteins

lipoGrade™: More hydrophobic (lipophilic) than immunoGrade™, for the attachment of hydrophobic molecules, for example progesterone and lipoproteins

immunoassay plates at a glance96 and 384 well formats•Standard, low volume or transparent •bottomTransparent, white or black•Different bottom shapes: U-, V-, F-, C-bottom for 96 well format•Clearly distinguishable by colour code: blue embossed alphanumeric coding for •96-well standard plates

promo code

13

Buy now to save 30%

new nunc surface, introductory price

30% off

BD Primaria™ cell culture productsA unique nitrogen-containing surface chemistry for enhanced cell culture.

The gases used in bD’s vacuum-gas plasma process to manufacture bD Primaria™ Cultureware contain both oxygen and ammonia, resulting in the incorporation of a variety of nitrogen-containing functional groups in addition to the negatively charged oxygen-containing groups found on traditional TC surfaces. This incorporation of nitrogen-containing cations has been correlated to attachment and spreading of primary endothelial cells in a clonal cell-growth assay. The complex surface on bD Primaria™ is homogeneous and stable, and is used by researchers to improve attachment, spreading, growth and differentiation of a variety of cell types, including:

Hepatocytes•Transfected COS-7•Transfected HeK-293•

promo code

15

20% off for all BD Primaria cellware

products

When chick embryo spinal cord neurons are cultured on bD Primaria™, growth is enhanced and extensive neurite development occurs (b). In this experiment, cells clumped and detached from traditional TC plates (A) after 20 days in culture, but remained viable and differentiated on bD Primaria™.

CHO•Primary SMC and Skeletal Muscle Cells•Primary Cardiac Myocytes•

Osteoblasts•Neuronal Cells•Endothelial Cells and many more•

‘A’

‘B’

Please ask for samples! [email protected]

50% off microslides

Purchase an ecm 2001 and receive

a free pack of microslides

(0,5 mm gap)

Description Qty/Pk cat. no. Price Promo priceECM 2001 Embryo Manipulation System - Includes accessories for embryo manipulation 1 732-0615P 189670,- -

ECM 2001 Electro Cell Fusion System - Includes accessories for cell fusion 1 732-0616P 217960,- -

ECM 2001 Electroporation System - Includes accessories for electroporation 1 732-0802P 183800,- -

ECM 2001 Generator Only 1 732-0617P 177000,- -

Microslides 450 0,5 mm gap 10 732-0004P 6480,- 3240,-

Microslides 450-1 1 mm gap 10 732-0005P 6480,- 3240,-

Microslide 453 3,2 mm gap 1 732-0006P 1880,- 940,-

Microslide 453-10 10 mm gap 1 732-0007P 1880,- 940,-

Description Lid sterile Pack cat. no. Price Promo price*MicroWell 96F Yes Yes 1/8 734-2245P 1630,- 1141,-

MicroWell 96U Yes Yes 1/8 734-2246P 1610,- 1127,-

MultiDish 24 Yes Yes 1/6 734-2247P 1230,- 861,-

MultiDish 12 Yes Yes 1/6 734-2248P 1220,- 854,-

Dish 10 cm Yes Yes 1/6 734-2249P 804,- 562,-

Dish 6 cm Yes Yes 5/30 734-2250P 3330,- 2331,-

Dish 3,5 cm Yes Yes 5/30 734-2251P 2770,- 1939,-

Description no. of wells

well shape/volume (µl)

Qty/Pk cat. no. Price Promo price

immunoGrade™

Transparent 96 U/330 100 735-2042P 1320,- 924,-

Transparent 96 V/350 100 735-2043P 1320,- 924,-

Transparent 96 F/350 100 735-2044P 1320,- 924,-

Transparent 96 C/350 100 735-2045P 1320,- 924,-

White 96 U/330 100 735-2046P 2610,- 1827,-

White 96 C/350 100 735-2047P 2610,- 1827,-

black 96 U/330 100 735-2048P 2610,- 1827,-

black 96 C/350 100 735-2049P 2610,- 1827,-

black/transparent 96 F/320 100 735-2050P 6360,- 4452,-

Transparent 384 F/100 50 735-2051P 2160,- 1484,-

White 384 F/100 50 735-2052P 2270,- 1589,-

black 384 F/100 50 735-2053P 2270,- 1589,-

hydroGrade™

Transparent 96 U/330 100 735-2054P 1580,- 1106,-

Transparent 96 F/350 100 735-2055P 1580,- 1106,-

Transparent 96 C/350 100 735-2056P 1580,- 1106,-

White 96 U/330 100 735-2057P 2870,- 2009,-

White 96 C/350 100 735-2058P 2870,- 2009,-

black 96 U/330 100 735-2059P 2870,- 2009,-

black 96 C/350 100 735-2060P 2870,- 2009,-

Transparent 384 F/100 50 735-2061P 2600,- 1820,-

White 384 F/100 50 735-2062P 2730,- 1911,-

black 384 F/100 50 735-2063P 2730,- 1911,-

lipoGrade™

Transparent 96 U/330 100 735-2064P 2220,- 1554,-

Transparent 96 V/350 100 735-2065P 2220,- 1554,-

Transparent 96 F/350 100 735-2066P 2220,- 1554,-

Transparent 96 C/350 100 735-2067P 2220,- 1554,-

White 96 U/330 100 735-2068P 3440,- 2408,-

White 96 C/350 100 735-2069P 3440,- 2408,-

black 96 U/330 100 735-2070P 3440,- 2408,-

black 96 C/350 100 735-2071P 3440,- 2408,-

White/transparent 96 F/330 100 735-2072P 7630,- 5341,-

black/transparent 96 F/330 100 735-2073P 7630,- 5341,-

Transparent 384 F/100 50 735-2074P 3130,- 2191,-

White 384 F/100 50 735-2075P 3280,- 2296,-

black 384 F/100 50 735-2076P 3280,- 2296,-

Transparent, low volume 384 F/30 50 735-2077P 4060,- 2842,-

White, low volume 384 F/30 50 735-2078P 6770,- 4739,-

black, low volume 384 F/30 50 735-2079P 6250,- 4375,-

White/transparent 384 F/120 50 735-2080P 7930,- 5551,-

black/transparent 384 F/120 50 735-2081P 7930,- 5551,-

BD Primaria™ Petri Dishes

Description Qty/Pk cat. no. Price Promo price35 x 10 mm easy-grip dish 200 734-0070P 529.42,- 423,-

60 x 15 mm standard dish 200 734-0071P 576.80,- 461,-

100 x 20 mm standard dish 200 734-0072P 1082.53,- 866,-

BD Primaria™ Flasks

Description Qty/Pk cat. no. Price Promo price25 cm2 flask with canted neck plug-seal caps 200 734-0075P 1611.95,- 1289,-

Vented, 0,2 µm caps 100 734-0073P 893.01,- 714,-

75 cm2 flask with straight neck plug-seal caps 100 734-0076P 1303.98,- 1043,-

Vented, 0,2 µm caps 100 734-0074P 1438.91,- 1151,-

BD Primaria™ multiwell Plates

Description Qty/Pk cat. no. Price Promo price6 well multiwell plate with lid 50 734-0077P 1017.64,- 814,-

24 well multiwell plate with lid 50 734-0078P 1090.77,- 872,-

96 well multiwell plate with lid, flat-bottom 50 734-0079P 1090.77,- 872,-

BioMarkeShop_12_DK.indd 3 10/10/2008 11:26:25 AM

Page 4: Shop! - 178.250.165.133178.250.165.133/ex/downloads/life_science/biomarke/0811/... · electrophoresis • It’s time for ... 2 Promotions in the VWrbioMarke SHOP may not be used

Place your order today! visit our website http://dk.vwr.comLocal price agreements apply.

offers valid from 1st november 2008 to 31st march 2009Promotions in the VWrbioMarke SHOP may not be used in combination, VWr reserves the right to refuse invalid orders.4

ISSUE 12 - NOVEMbEr 2008Shop!

order from the customer service centre or your vwr Primary Distributor:These offers cannot be used in conjunction with any other pricing agreements from VWR International. Prices exclude VAT.

VWr - bie & berntsenTransformervej 82730 Herlev

Place your order today!Tel: 43 86 87 88Fax: 43 86 87 90http://dk.vwr.come-mail: [email protected]

simple, reliable concentrating and desalting with Pall nanosep devicesNanoSep centrifugal devices are ideal for rapid processing of samples of between 50 to 500 µl. The typical recoveries obtained using Nanosep devices are greater than 90%. Pall NanoSeps are constructed of low-binding polypropylene and contain the Omega membrane. The NanoSep range can be used for a number of applications including:

Concentrate, purify, and desalt oligonucleotides, •DNA and rNAClean up labelling and PCr reactions•Isolate DNA from agarose gel slices•Separate oligonucleotides and rNA from •acrylamide gelsSupplied in packs of 500•

sample preppromo

code20

Buy 3 identical products and pay for only 2

nALGene® high-speed centrifuge tubes and bottles“Spin with confidence”

Designed for most common high-speed centrifuge rotors, the NALGENE® Oak ridge centrifuge tubes and bottles with screw closure are leakproof up to their maximum rated rCF (50 000 x g for tubes – refrigerated or not), giving you peace of mind when spinning your valuable samples down.

All NALGENE® centrifuge tubes are re-useable and made out of autoclavable plastics. Choose between polypropylene copolymer (PPCO) tubes for most common applications; crystal-clear polycarbonate (PC) tubes when visual clarity is desired; and Teflon FEP tubes for samples in aggressive organic solvents.

To find the tube or bottle that fits your rotor, contact your local VWr sales specialist.

promo code

16

vwr collection incubating orbital shakerExclusive “Optiflow” forced ventilation system offers exceptional temperature uniformity. Microprocessor controls with digital displays for speed, temperature and time, plus user calibrations for temperature and speed. rS232 serial port for data logging and unit control.

Maximum load - 15,9 kg•Speed range - 15 to 500 rpm with an accuracy of +/- 1%•Temperature range - ambient +5 °C to 65 °C•Temperature uniformity is +/- 0,5 ºC at 37 ºC•Timer from 1 second to 9999 minutes•Orbit - 19 mm •

promo code

18

DKK 1000 off the list price plus free platform for mounting

flask clamps and test tube racks

2 ml cryogenic sterile cryule® vialsSpecially designed for safe effective cryogenic cell storage •Freestanding on the bench-lock into rack•External thread allows maximum vial capacity use and helps to avoid contamination•White marking area and colour-coded caps•Case of 500 Cryule• ® Vials packed in 10 sealed bags of 50 eachPolypropylene, dia x ht 12 x 46 mm•

sAve now… 20% oFF the reduced price!

promo code

17

ready-to-use Dialysis DeviceSpectrum introduces the next generation (G2) in ready-to-use laboratory dialysis devices featuring Ultra-pure biotech Cellulose Ester (CE) Membrane.

95 – 98% sample recovery•Highest membrane purity•Superior handling & leak prevention•Volume specific dilution control•Complete & ready-to-use packaging•

the dialysis device with the best sample recovery, purity & dilution controlThe new Float-A-Lyzer® G2 is a low protein-binding synthetic membrane available in 4 precise MWCOs with no heavy metal and sulphide contaminants. Only G2 assures a 95-98% sample recovery while maintaining 99% sample purity and <1% sample dilution and is packaged dry.

promo code

21

Bigger pack size! Float-A-Lyzer® G2

comes in packs of 12 for the same price as the old pack of 10!

25% off

easy to use custom oligonucleotide online order tool!order now on www.vwr.com and mention the code vwr38 to claim your 1 GB-usB stick*Please contact your local VWr LifeSciences specialist for more information on how to order on the lab shop.

promo code

19

Free 1 GB-usB stick**terms and conditions:Offer only valid for orders placed from 1st November 2008 till 31st May 2009 through the VWr order page. Offer only available for the first 100 customers.Void when prohibited by law or company/ organisation.

S C I E N T I F I C

microplate readingmultiskan Fc, a reliable and easy to use microplate photometerThermo Scientific Multiskan FC is a new generation microplate photometer designed with 30 years experience in microplate photometry. Multiskan FC is a reliable and robust instrument for a wide variety of photometric research and routine applications offering ease of use via the large colour display, visual user interface and multiple language options.

Email [email protected] and supply us with the serial number of the instrument you purchased, state the wavelength of the filter you want, and we will send you your free filter.

promo code

22

Purchase a multiskan Fc and get an additional

filter* Free!

Contact your local VWr office and ask for a copy of the Nanosep Centrifugal Devices - Protocols for Use Guide PN 32989, or alternatively view the online version at www.pall.com/lab.

S C I E N T I F I C

Ask your local vwr sales office

*Offer valid for standard filters340,414,492,540,550, 570, 595, 630, 650,

690 nm

nALGene Product Description size (ml) max. g-force at 4 °c Qty/Pk cat. no. Price Promo priceoak ridge centrifuge tube

PPCO (3119 type) 10 50 000 10 525-2241P 293,- 219,-

PPCO (3119 type) 30 50 000 10 525-2243P 337,- 252,-

PPCO (3119 type) 50 50 000 10 525-2244P 419,- 314,-

PC (3118 type) 10 50 000 10 525-2231P 365,- 273,-

PC (3118 type) 30 50 000 10 525-2233P 395,- 296,-

PC (3118 type) 50 50 000 10 525-2234P 478,- 358,-

PC (3118 type) 85 50 000 10 525-2235P 542,- 433,-

FEP (3114 type) 10 50 000 2 525-2211P 258,- 193,-

FEP (3114 type) 30 50 000 2 525-2212P 394,- 295,-

FEP (3114 type) 50 50 000 2 525-2213P 440,- 330,-

centrifuge Bottle with screw cap

PPCO (3120 type) 250 27 500 4 525-2311P 204,- 155,-

PPCO (3120 type) 500 4 800 4 525-2313P 234,- 175,-

PPCO (3120 type - Ht: 175 mm) 1000 7 100 4 525-2316P 471,- 353,-

PPCO (3120 type - Ht: 168 mm) 1000 7 100 4 525-2315P 471,- 353,-

PC (3122 type) 250 27 500 4 525-2331P 300,- 225,-

PC (3122 type) 500 13 700 4 525-2333P 450,- 337,-

PC (3122 type) 1000 7 100 4 525-2335P 637,- 477,-

FEP (3127 type) 250 4 000 1 525-2341P 583,- 437,-

Description cat. no Price Promo priceIncubating Orbital Shaker with a cord and 33 x 27,9 cm platform 444-7100P 29000,- 28000,-

cap colour cat. no. reduced price Promo priceWhite 216-0395P 1310,- 1048,-

red 216-3020P 1310,- 1048,-

Pink 216-3021P 1310,- 1048,-

Yellow 216-0398P 1310,- 1048,-

Green 216-0401P 1310,- 1048,-

blue 216-3022P 1310,- 1048,-

sample vol. (ml) mwco (Daltons) cat. no. Price1

3 500 – 5 000

734-3573P 1220,-

5 734-3577P 1330,-

10 734-3581P 1330,-

1

8 000 – 10 000

734-3574P 1220,-

5 734-3578P 1330,-

10 734-3582P 1330,-

1

20 000

734-3575P 1220,-

5 734-3579P 1330,-

10 734-3583P 1330,-

1

100 000

734-3576P 1220,-

5 734-3580P 1330,-

10 734-3584P 1330,-

colour size (K) cat. no. Price

Grey 3 516-0584P 9890,-

blue 10 516-8492P 9890,-

red 30 516-8503P 9890,-

Clear 100 516-8521P 9890,-

Orange 300 516-8532P 9890,-

Description cat. no. PriceMultiskan FC microplate photometer (405, 450 and 620 nm filters included) 736-0355P 38370,-

Multiskan FC microplate photometer with incubator (405, 450 and 620 nm filters included) 736-0356P 55410,-

BioMarkeShop_12_DK.indd 4 10/10/2008 11:26:34 AM