Shimadzu · capabilities will elevate your lab to the next level. Designed with the Analyst in Mind...
Transcript of Shimadzu · capabilities will elevate your lab to the next level. Designed with the Analyst in Mind...
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C184-E045
Gas Chromatograph
The Next Industry StandardNexis GC-2030, Shimadzu's premier gas chromatograph, offers a modern approach to a classic chromatographic technique. Designed with the user in mind, new innovative features, exceptional performance and high-throughout capabilities will elevate your lab to the next level.
Designed with the Analyst in Mind
An advanced interface enables intuitive operation with clear graphics. Shimadzu's latest tool-free
maintenance technology makes daily maintenance easy.
World’s Highest*1 Sensitivity and Reproducibility
Achieves the world’s highest*1 sensitivity on the all of the detectors, such as FID and BID. The advanced
flow controller (AFC) enhances reliability with excellent repeatability.
Exceptional Expandability and Productivity
Nexis GC-2030 can be customized to meet a customers' specific requirements and needs. Options and
functions to use hydrogen carrier gas safely in high-speed analysis maximize analysis productivity.
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Information at Your Finger-tipsAnalysts will benefit from the touch panel interface, which features
clear graphics that display information instantly whenever needed.
The user-friendly interface leaves the operator free to focus on
obtaining optimal analytical results.
Main settings controllable via the touch panel on the GC unit:
• Analytical conditions• Self-diagnostics• Automatic carrier gas leak check• Chromatogram display, etc.
Tool-free Column InstallationClickTek connectors*2 make tool free column installation a snap.
The click sensation felt when finished attaching the column
provides a more reliable connection and ensures a better seal
under all operating conditions.
*2 Optional
ClickTek Connector
One Touch Inlet Maintenance The injection port can be opened or closed without tools by simply
sliding the ClickTek lever. Replace the insert, slide the lever and feel
the click for a leak-free install every time.
ClickTek Nut
High-Sensitivity Detectors Support a Wide Variety of Analyses
The jet and collector structure on the flame ionization detector
(FID-2030) has been optimized to provide improved performance.
Noise levels were also decreased by improving the stability of the
signal processor and flow controller. This results in the world's most*1
sensitive FID. This makes the Nexis GC-2030 the best choice to
measure residual solvents in pharmaceuticals.
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0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 min
1. 1,1-Dichloroethane2. 1,1,1-Trichloroethane3. Carbontetrachloride4. Benzene5. 1,2-Dichloroethane
Analysis of Trace Residual Solvents in PharmaceuticalsUsing Headspace GC, Class 1 Standard Solution
GC Systems Customized for Specific NeedsThe Nexis GC-2030 provides powerful support for configuring custom
GC systems tailored to user needs. These systems are adjusted and
tested at the factory for the given application before shipment, so
they are ready to use for measurements as soon as they are delivered.
That means no time is required for developing methods after the
system arrives. Two TCD detectors and one FID detector can be
installed at the same time. An optional valve box can be added to
control up to eight valves from the original four.
Examples of System GC Configurations
Gasoline analysis system
This system is able to measure specific substances in gasoline, such as oxygenates.
Natural gas analysis system
This system is able to analyze components in natural gas, such as shale gas.
Inorganic gasanalysis system
This system is able to measure hydrogen and various other inorganic gases.
Hydrocarbonanalysis system
This system is able to measure hydrocarbons that are generated, such as from catalyst reactions.
Refinery gas analysis system
This system is able to analyze components in gas from petroleum refineries.
Public utility natural gas analysis system
This system is able to calculate calorific values from measurements of natural gas.
*1 As of May 2017, according to a Shimadzu survey
© Shimadzu Corporation, 2017
For Research Use Only. Not for use in diagnostic procedures. This publication may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol “TM” or “®”. Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.
The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.www.shimadzu.com/an/
First Edition: May 2017, Printed in Japan 3655-05713-30ANS4
1 Introduction
The HS-GC-FID method is used to test for residual solvents in pharmaceuticals, but GC-MS is useful for identifying peaks in close
proximity or for qualifying unknown peaks. However, to qualify peaks detected by GC-FID using GC-MS requires matching
chromatogram patterns. The advanced flow controller (AFC) in GC-2010 Plus systems includes constant linear velocity control as
standard functionality. This allows achieving similar retention time and separation patterns in GC-FID and GC-MS chromatograms by
specifying the same linear velocity setting, provided the columns are identical or columns with the same phase ratio are used.
2 Analytical Conditions to Test for Residual Solvents in Pharmaceuticals
Testing for residual solvents in pharmaceuticals involves using a separation column with a 0.53 mm (or 0.32 mm) internal diameter,
30 m length, and 3.0 µm (or 1.8 µm) thick cyanopropyl phenyl-based liquid phase and separation conditions with a linear carrier gas
speed of 35 cm/sec (Procedure A). However, those separation conditions cannot be achieved using GC-MS, due to the negative
pressure (vacuum) at the column outlet. Therefore, the typical method used to obtain similar chromatogram patterns is to downsize
the column to one with a small internal diameter, so that the phase ratio (ratio of column internal diameter to film thickness) is the
same.
Control by the AFC takes into consideration the difference in column outlet pressure between GC and GC-MS. For HS-GCMS analysis,
it simply requires changing the column and specifying a 35 cm/sec linear velocity in the method parameter selection window in
GCMSsolution. During analysis, the AFC controls the carrier gas automatically, which eliminates the inconvenience of having to
adjust pressure or other settings.
GCMS-QP2020 + HS-20 + FID-2010 Plus DetectorBy including an FID detector in the HS-GCMS system, either
analytical method can be used. Contact a Shimadzu representative
regarding changing the system configuration, which can be
modified to accommodate various user requirements.
Using GCMS to Testfor Residual Solvents in Pharmaceuticals
C146-E338
This results in similar chromatogram patternsusing the same linear velocity of 35 cm/sec.
SH Rxi-624sil MS0.32 mm I.D., 30 m long, and 1.8 µmfilm thickness
SH Rxi-624sil MS0.25 mm I.D., 30 m long, and 1.4 µmfilm thickness
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3 Comparison of GC-FID and GC-MS Chromatograms
After using a GC-FID system to acquire data from a Class 2 standard solution A, data were acquired using a GC-MS system with the
same linear velocity condition (35 cm/sec). Then the resulting chromatograms were compared. The comparison shows that the shift
in retention times between FID and MS data was 0.02 minutes (1.2 seconds) for acetonitrile, which elutes early, and for cumene,
which elutes late. In addition, both chromatograms were similar, with approximately the same separation patterns.
The GC-MS data showed a peak at roughly the same retention time as for the unknown peak detected by the GC-FID system. This
peak can be identified easily by qualitative analysis, such as by displaying the mass spectrum and using an MS spectral library to
search for a similar peak pattern.
4 Summary
If a HS-GC-MS system is used for qualitative analysis in testing for residual solvents in pharmaceuticals, it is important that the
chromatogram pattern obtained is similar to the chromatogram obtained from HS-GC-FID analysis. The constant linear velocity
control mode for the AFC unit included in Shimadzu GC-MS systems can be used in combination with a Shimadzu HS-20 headspace
sampler. Even if the different-sized columns are used for HS-GC-FID and HS-GC-MS analysis, chromatograms with similar retention
times and separation patterns can be obtained easily by using columns with the same phase ratio and by specifying the same linear
velocity setting, which means GC-MS data can easily be used for qualitative analysis.
© Shimadzu Corporation, 2017
www.shimadzu.com/an/
For Research Use Only. Not for use in diagnostic procedures. This publication may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.Company names, products/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation, its subsidiaries or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services, whether or not they are used with trademark symbol “TM” or “®”.Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.
The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.
Printed in Japan 3655-02707-20AIT
Acetonitrile(4.05 min)
Acetonitrile(4.07 min)
Cumene(29.13 min)
FID
MS
Cumene(29.15 min)
Qualitative analysis based on the mass spectrum displayed
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Gas Chromatography Mass Spectrometry
Analysis of Residual Solvents – Class 1, Class 2A, Class 2B – in Pharmaceuticals Using Headspace-GC/MS
LAAN-A-MS-E034
Residual solvents in pharmaceuticals are defined as organic volatile chemicals that are used or produced in the manufacture of drug substances or excipients, or in the preparation of drug products. Residual solvents are classified and managed as Class 1 to Class 3 substances, depending on the risk they pose to human health.According to the USP, "General Chapter <467> Residual Solvents" method, analysis of these residual solvents is to be conducted by the headspace GC-FID method (HS-GC). However in this application, we investigated using headspace-GC/MS (HS-GC/MS) according to Procedure A. Using the HS-GC method, measurement is to be performed as three separate analyses, which are required to achieve chromatographic separation within the three different classes of compounds, Class 1, Class 2A, and Class 2B. However, using HS-GC/MS, complete chromatographic separation is not necessary, so all the compounds can be analyzed in a single run. In addition, compound confirmation and qualitative information of unknown peaks can also be obtained.
n Sample PreparationThe Class 1, Class 2A, and Class 2B aqueous standard solutions were prepared so that the concentrations become the same as standard solution designated in the "USP <467> Residual Solvents" method.
Headspace Sampler : HS-20
Gas Chromatograph Mass Spectrometer : GCMS-QP2010 UltraHSMode : Loop (Volume: 1 mL)Oven Temperature : 80 °CSample Line Temperature : 150 °CTransfer Line Temperature : 150 °CGas Pressure for Vial Pressurization : 100 kPaVial Equilibrating Time :60 minVial Pressurizing Time :2.0 minPressure Equilibrating Time :0.1 minLoad Time :0.1 minLoad Equilibrating Time :0.1 minInjection Time :0.5 minNeedle Flush Time :5.0 minGC
Column : Rxi-624sil MS (30 m × 0.25 I.D.,1.4 µm)
Injection Mode :Split Split Ratio :1:30Control Mode :Constant linear velocity (35 cm/sec)Oven Temperature :40 °C (20 min) → 10 °C/min →
240 °C (20 min)
MSIon Source Temperature : 200 °CInterface Temperature : 250 °CSCAN Range :m/z 29 ~ 200SIM Conditions : Table 1Event Time : SIM 0.2 sec, SCAN 0.3 sec
Compound Name Target Ident 1 Ident 2
Class 1 1,1-Dichloroethene 61 96
1,1,1-Trichloroethane 97 99
Carbon Tetrachloride 117 119
Benzene 78 77 51
1,2-Dichloroethane 62 64
Class 2A Methanol 31 29
Acetonitrile 40 39
Methylene chloride 84 86
trans-1,2-Dichloroethene 96 61
cis-1,2-Dichloroethene 96 61
Tetrahydrofuran 72 42
Cyclohexane 84 56
Methylcyclohexane 98 83
1,4-Dioxane 88 58
Toluene 91 92
Chlorobenzene 112 77
Ethylbenzene 91 106
m,p-Xylene 91 106
o-Xylene 91 106
Class 2B n-Hexane 86 56
Nitromethane 30 46
Chloroform 83 85
1,2-Dimethoxyethane 45 29
Trichloroethene 130 132
Pyridine 79 52
2-Hexanone 58 100
Tetralin 104 132n Analytical Conditions
Table 1 SIM Monitoring Ions
n ResultsFig. 1 shows Total Ion Chromatogram (TIC) for the USP Class 1 compounds. Fig. 2 and 3 are the TICs for Class 2A and 2B compounds, respectively. Peaks that cannot be identified in the TIC and peaks that completely or partially co-elute are shown in the extracted ion chromatogram (EIC). Due to the selectivity of the GC/MS, good separation was obtained by using the SIM acquisition mode. Fig. 4, 5 and 6 show the EIC/SIM chromatograms of the individual components. Good peak shapes we re ob ta ined fo r mos t o f the compounds. In addition, an improved signal-to-noise ratio (S/N) was obtained for CCl4 using the HS-GC/MS method, compared to that obtained by the HS-GC method. Repeatability using the HS-GC/MS SIM mode yielded an RSD of 1.3 to 3.9 % (Tables 2, 3, and 4).
n ConclusionUsing the HS-GC/MS method, simultaneous analysis of USP <467> Class 1, Class 2A, and Class 2B compounds was demonstrated without compromising separation, repeatability, or analysis accuracy.Note: Measurement of residual solvents in pharmaceuticals using HS-
GC/MS has not been adopted as an official method.
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Fig. 2 TIC Chromatogram of Class 2A Solvents
Fig. 3 TIC Chromatogram of Class 2B Solvents
Car
bon
tetra
chlo
ride
tran
s
cis
m,p
o
n
Fig. 1 TIC Chromatogram of Class 1 Solvents
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1,1-Dichloroethene 1,1,1-Trichloroethane Carbon tetrachloride Benzene 1,2-Dichloroethane
EIC
SIM
3.5 4.0
2.5
5.0
(×1,000)
61.0096.00
8.5 9.0
2.5
5.0
7.5(×1,000)
99.0097.00
9.0 9.5
1.0
2.0
(×1,000)
119.00117.00
10.0 10.5
2.5
5.0
(×1,000)
51.0077.0078.00
10.0 10.5
0.5
1.0
1.5(×1,000)
64.0062.00
3.5 4.0
2.5
5.0
7.5
(×1,000)
61.0096.00
8.5 9.0
2.5
5.0
7.5
(×1,000)
99.0097.00
9.0 9.5
1.0
2.0
3.0(×1,000)
119.00117.00
10.0 10.5
2.5
5.0
(×1,000)
51.0077.0078.00
10.0 10.5
0.5
1.0
1.5
(×1,000)
64.0062.00
4.0 4.5
2.5
0.0
(×100,000)
86.0084.00
4.5 5.0
0.5
1.0
(×1,000,000)
61.0096.00
7.0 7.5
2.5
5.0
7.5
(×100,000)
61.0096.00
8.5 9.0
1.0
2.0
(×1,000,000)
56.0084.00
14.5 15.0
2.5
5.0
7.5
(×100,000)
83.0098.00
15.5 16.0
1.0
2.0
(×1,000)
58.0088.00
22.0 22.5
1.0
2.0
(×1,000,000)
92.0091.00
27.0 27.5
1.0
2.0
(×1,000,000)
106.0091.00
27.5 28.0
1.0
2.0
(×1,000,000)
106.0091.00
4.0 4.5
2.5
0.0
(×10,000)
39.0040.0041.00
2.0 2.5
1.0
2.0
3.0(×10,000)
29.0031.00
7.5 8.0
0.5
1.0
(×100,000)
42.0072.00
EIC
EIC
SIM
SIM
Methanol
Toluene
Acetonitrile Dichloromethane trans -1,2-dichloroethene cis -1,2-dichloroethene
Tetrahydrofuran Cyclohexane
EIC
m,p-XyleneChlorobenzene Ethylbenzene o-Xylene
SIM
2.0 2.5
1.0
2.0
3.0(×10,000)
29.0031.00
4.0 4.5
0.5
1.0
(×10,000)
39.0040.00
4.0 4.5
2.5
0.0
(×100,000)
86.0084.00
4.5 5.0
0.5
1.0(×1,000,000)
61.0096.00
7.0 7.5
2.5
5.0
(×100,000)
61.0096.00
7.5 8.0
0.5
1.0(×100,000)
42.0072.00
8.5 9.0
1.0
2.0
(×1,000,000)
56.0084.00
14.5 15.0
2.5
5.0
7.5(×100,000)
83.0098.00
15.5 16.0
1.0
2.0
(×1,000)
58.0088.00
22.0 22.5
1.0
2.0
(×1,000,000)
92.0091.00
26.5 27.0
0.5
1.0
(×1,000,000)
77.00112.00
27.0 27.5
1.0
2.0(×1,000,000)
106.0091.00
27.5 28.0
1.0
2.0(×1,000,000)
106.0091.00
28.0 28.5
2.5
0.0
(×1,000,000)
106.0091.00
26.5 27.0
0.5
1.0
(×1,000,000)
77.00112.00
28.0 28.5
2.5
0.0
(×1,000,000)
106.0091.00
1,4-DioxaneMethylcyclohexane
Fig. 4 EIC/SIM Chromatograms of Class 1 Solvents
Fig. 5 EIC/SIM Chromatograms of Class 2A Solvents
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For Research Use Only. Not for use in diagnostic procedures.The content of this publication shall not be reproduced, altered or sold for any commercial purpose without the written approval of Shimadzu. The information contained herein is provided to you "as is" without warranty of any kind including without limitation warranties as to its accuracy or completeness. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication. This publication is based upon the information available to Shimadzu on or before the date of publication, and subject to change without notice.
© Shimadzu Corporation, 2014www.shimadzu.com/an/
M268
First Edition: Oct. 2014
EIC
SIM
n-Hexane
Tetralin2-Hexanone
Nitromethane
Pyridine
5.0 5.5
2.5
5.0
7.5(×10,000)
56.0086.00
7.0 7.5
2.5
5.0
7.5
(×100)
46.0030.00
8.0 8.5
2.5
5.0
(×10,000)
85.0083.00
10.0 10.5
2.5
5.0
(×1,000)
29.0045.00
13.0 13.5
2.5
0.0
(×10,000)
132.00130.00
21.5 22.0
1.0
2.0
3.0(×1,000)
52.0079.00
25.0 25.5
0.5
1.0
1.5(×10,000)
100.0058.00
5.0 5.5
2.5
5.0
7.5
(×10,000)
56.0086.00
7.0 7.5
2.5
5.0
7.5
(×100)
46.0030.00
8.0 8.5
2.5
5.0
(×10,000)
85.0083.00
10.0 10.5
2.5
5.0
7.5(×1,000)
29.0045.00
13.0 13.5
2.5
5.0(×10,000)
132.00130.00
34.5 35.0
2.5
0.0
(×100,000)
132.00104.00
21.5 22.0
1.0
2.0
3.0
(×1,000)
52.0079.00
25.0 25.5
1.0
0.0
(×10,000)
100.0058.00
34.5 35.0
2.5
0.0
(×100,000)
132.00104.00
EIC
SIM
Trichloroethene1,2-DimetoxyethaneChloroform
Fig. 6 EIC/SIM Chromatograms of Class 2B Solvents
Conc. Area RSD (%)
Compound Name (µg/mL) EIC SIM
Class 1 1,1-Dichloroethene 0.018 2.42 2.79
1,1,1-Trichloroethane 0.033 1.86 2.61
Carbon tetrachloride 0.045 1.64 1.62
Benzene 0.064 1.52 2.01
1,2-Dichloroethane 0.085 2.21 2.30
Conc. Area RSD (%)
Compound Name (µg/mL) EIC SIM
Class 2B n-Hexane 0.52 3.46 3.38
Nitromethane 0.82 3.72 2.44
Chloroform 1.97 2.48 2.67
1,2-Dimethoxyethane 0.42 2.62 2.74
Trichloroethene 0.42 1.23 1.56
Pyridine 1.67 2.94 3.29
2-Hexanone 0.83 0.83 1.34
Tetralin 0.65 1.87 1.77
Conc. Area RSD (%)
Compound Name (µg/mL) EIC SIM
Class 2A Methanol 3.03 4.26 3.83
Acetonitrile 2.85 2.74 3.29
Methylene Chloride 27.0 2.24 2.78
trans-1,2-Dichloroethene 14.6 1.91 2.60
cis-1,2-Dichloroethene 5.05 1.93 2.49
Tetrahydrofuran 3.12 1.87 2.12
Cyclohexane 24.1 1.67 2.27
Methylcyclohexane 8.72 1.33 1.69
1,4-Dioxane 6.15 3.13 2.54
Toluene 7.00 1.17 1.56
Chlorobenzene 2.92 1.30 1.28
Ethylbenzene 1.47 1.32 1.41
m,p-Xylene 2.48 1.07 1.41
o-Xylene 10.3 1.23 1.66
Table 2 Repeatability of Peak Area of Class 1 Solvents (n = 6)
Table 4 Repeatability of Peak Area of Class 2B Solvents (n = 6)
Table 3 Repeatability of Peak Area of Class 2A Solvents (n = 6)
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APC PressureControl
Splitting Unit
ApplicationNews
No.M272
Gas Chromatography Mass Spectrometry
Analysis of Residual Solvents in Pharmaceuticals Using Headspace GC-FID/MS Detector Splitting System
LAAN-A-MS-E038
Table 1 Analytical Conditions
Headspace Sampler : HS-20
GCMS : GCMS-QP2020Hydrogen Flame Ionization Detector Splitting System
: FID-2010Plus
HSMode : Loop (volume 1 mL)Oven Temp. : 80 °CSample Line Temp. : 90 °CTransfer Line Temp. : 105 °CGas Pressure for Vial Pressurization : 76.4 kPaVial Equilibrating Time : 45 minVial Pressurizing Time : 2.0 minPressure Equilibrating Time : 0.1 minLoad Time : 0.5 minLoad Equilibrating Time : 0.1 minInjection Time : 0.5 min
Needle Flushing Time : 15.0 minAPC Pressure : 20 kPa
GC
Column : SH Rxi-624sil MS(30 m × 0.32 mm I.D., 1.8 µm)
Injection Mode : Split (split ratio 1:5)Control Mode : Constant Pressure (89.4 kPa) Carrier Gas : HeOven Temp. : 40 °C (20 min) → 10 °C/min →
240 °C (20 min)Restrictor (FID) : 1.1 m × 0.25 mmRestrictor (MS) : 1.5 m × 0.20 mmAPC Pressure : 20 kPa
FID
Temp. : 250 °CMake-Up Flowrate : 30 mL/min (He)Hydrogen Flowrate : 40 mL/minAir Flowrate : 400 mL/min
MS
Ion Source Temp. : 200 °CInterface Temp. : 250 °CSCAN Range : m/z 29 to 250Event Time : 0.3 sec
Headspace gas chromatography with flame ionization detection (GC-FID) is often used for residual solvent testing of pharmaceuticals, though the qualitative power of this method is not particularly high. Because gas chromatography mass spectrometry (GC/MS) utilizes MS to perform qualitative analysis based on mass spectra, GC/MS can be used to estimate and identify individual peaks detected in the expected vicinity of a target solvent as well as other unknown peaks.We describe an example of residual solvent test of a pharmaceutical using a detector splitting system that simultaneously obtains FID and MS data in a single measurement.
n Sample PreparationAccording to Water-Soluble Articles, Procedure A, in USP <467>, we prepared a class 1 standard solution, class 2 standard solution A, class 2 standard solution B, test solution, and class 1 system suitability solution. An active pharmaceutical ingredient was used for the test solution sample.
n Analytical ConditionsThe image of the Shimadzu GCMS-QP2020/FID detector splitting system is shown in Fig. 1, and analytical conditions are shown in Table 1. Headspace conditions were determined based on USP <467>. The column outlet was split between FID and MS, and MS was performed in scanning mode. Using Shimadzu's Advanced Flow Technology Software to determine the splitting ratio, the flowrate ratio was optimized to FID:MS of 1:1.
Fig. 1 System Image11
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n ResultsFig. 2 to 5 show the FID and MS chromatograms obtained for class 1 standard solution, class 2 standard solution A, class 2 standard solution B, and class 1 system suitability solution.
3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0
0
1000
2000
3000
4000
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1, 1
-Dic
hlor
oeth
ene
1, 1
, 1-T
richl
oroe
than
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Car
bon
Tetr
achl
orid
e
Benz
ene
1, 2
-Dic
hlor
oeth
ane
MS (Scan) FID
2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5
25000
50000
75000
100000
125000
150000
175000
200000
225000
Met
hano
l
Ace
toni
trile
Met
hyle
neC
hlor
ide
tran
s-1,
2-D
ichl
oroe
then
e
cis-
1, 2
-Dic
hlor
oeth
ene
Tetr
ahyd
rofu
ran
Cyc
lohe
xane
Met
hylc
yclo
hexa
ne
1, 4
-Dio
xane
Tolu
ene
Chl
orob
enze
neEt
hylb
enze
ne
m,p
-Xyl
ene
o-X
ylen
eC
umen
e
5.0 10.0 15.0 20.0 25.0 30.0 35.0
2500
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N-H
exan
e
Nitr
omet
hane
Chl
orof
orm
1, 2
-Dim
etho
xyet
hane Tr
ichl
oroe
then
e
Pyrid
ine 2-
Hex
anon
e
Tetr
alin
Fig. 2 Chromatograms of Class 1 Standard Solution
Fig. 3 Chromatograms of Class 2 Mixture A Standard Solution
Fig. 4 Chromatograms of Class 2 Mixture B Standard Solution
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No.M272
MS(Scan) FID
3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0
1, 1
-Dic
hlor
oeth
ene
1, 1
, 1-T
richl
oroe
than
e
Car
bon
Tetr
achl
orid
e
Benz
ene
1, 2
-Dic
hlor
oeth
ane
Fig. 5 Chromatograms of Class 1 System Suitability Solution
To check the mass spectra of the peaks detected by FID, the peak retention times in chromatograms obtained by FID and MS must match as closely as possible. Looking at Fig. 2 to 4 show all the peak retention times are lined up, from the earliest to the latest constituent.When using a detector splitting system, the two detectors must detect the same peaks detected by normal gas chromatography. In other words, detector splitting systems are expected to have the equivalent system performance as a normal analytical system. Procedure A in USP <467> states the two items below concerning system suitability. We attempted to confirm the two items below for the detector splitting system, and for the repeatability of class 1 standard solution analysis.
(1) Detector confirmationThe S/N ratio of 1, 1, 1-trichloroethane in class 1 standard solution is 5 or higher; the S/N ratio of each peak in class 1 system suitability solution is 3 or higher.
(2) System performanceThe peak resolut ion between acetonit r i le and dichloromethane in class 2 standard solution is 1.0 or higher.
The results (FID S/N ratios) of analyzing class 1 standard solution and class 1 system suitability solution with the detector splitting system are shown in Table 2, and the repeatability results (FID repeatability) of analyzing class 1 standard solution are shown in Table 3. These results show the detector sp l i t t ing system meets the performance required of a standard system. The peak resolution of acetonitrile and dichloromethane in class 2 standard solution was 2.37, showing this system is also suitable in terms of resolution.
Table 2 Signal-to-Noise Ratio in Class 1 Standard Solution and System Suitability Solution
Table 3 Repeatability in Class 1 Standard Solution (n=6)
Compound Standard solutionSolution for system
suitability test1, 1-Dichloroethene 221.9 141.41, 1, 1-Trichloroethane 117.6 82.2Carbon tetrachloride 10.2 7.6Benzene 106.3 56.81, 2-Dichloroethane 26.4 14.2
Compound Relative standard deviation (%)1, 1-Dichloroethene 1.61, 1, 1-Trichloroethane 2.2Carbon tetrachloride 1.8Benzene 3.51, 2-Dichloroethane 2.9
13
5.0 10.0 15.0 20.0 25.0 30.0 35.0
5.0 10.0 15.0 20.0 25.0 30.0 35.0
5.0 10.0 15.0 20.0 25.0 30.0 35.0
5.0 10.0 15.0 20.0 25.0 30.0 35.0
ab
d
c
Class1 Standard(FID)
Class2A Standard(FID)
Class2B Standard(FID)
Test (FID)
Test (MS)
50 100 150 200 2500
50
10056.031.0 n-Butanol
50 100 150 200 2500
50
10043.0
29.0
61.088.1
Ethyl acetate
50 100 150 200 2500
50
10091.1
106.2
77.051.0
o-Xylene
50 100 150 200 2500
50
10057.0
41.0 87.173.1 130.2
Dibutylether
ApplicationNews
No.
© Shimadzu Corporation, 2016
For Research Use Only. Not for use in diagnostic procedure. This publication may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country. The content of this publication shall not be reproduced, altered or sold for any commercial purpose without the written approval of Shimadzu. Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol “TM” or “®”. Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.
The information contained herein is provided to you "as is" without warranty of any kind including without limitation warranties as to its accuracy or completeness. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication. This publication is based upon the information available to Shimadzu on or before the date of publication, and subject to change without notice.
www.shimadzu.com/an/
M272
First Edition: Jul. 2016
The results (chromatograms) of analyzing active pharmaceutical ingredients in the detector splitting system are shown in Fig. 6, and the mass spectra of detected peaks are shown in Fig. 7 to 9. Peaks a and b, based on their respective mass spectra (Fig. 7 and 8), were estimated to be ethyl acetate and butanol. Both these constituents are low toxicity class 3 solvents.
Though its peak strength is smaller than that observed in the standard solution, a peak was also detected at the elution position of o-xylene (c). Checking the mass spectrum of this peak (Fig. 9) showed it differed from the mass spectrum of xylene (peak d, Fig. 10), and was estimated to be dibutyl ether.
Fig. 6 Chromatograms of Standard Solutions and Test Solutions
Fig. 7 Mass Spectrum of Peak a Fig. 9 Mass Spectrum of Peak c
Fig. 8 Mass Spectrum of Peak b Fig. 10 Mass Spectrum of Peak d
n ConclusionAn FID and MS detector splitting system obtains FID and MS data simultaneously in a single analysis, and can be used as a simpler method of confirming constituent identity. This system shows promise for use in residual solvent testing of pharmaceuticals.Note: Reference USP <467>This data was obtained by a method that does not conform to the pharmacopoeia, as analytical conditions based on USP <467> was modified before use.
14
C146-E300
Gas Chromatograph Mass Spectrometer
Ultra High Speed GC-MS That Offersa Dramatic Leap in Sensit iv i ty and Product iv i ty
Provides Higher Sensitivity and Reduced Operation Costs
The high-sensitivity, high-speed capability analysis is achieved, using helium, hydrogen or nitrogen as the
carrier gas and the ionization is easily switched without stopping the MS.
Dramatic Improvement in the Ef�ciency of Multicomponent Simultaneous Analysis
The GCMS Insight software package supports everything from method creation to analysis.
Databases with Retention Indices to Support Analysis
Databases are available to satisfy a variety of needs, including environmental analysis and food analysis, etc.
Con�gure Optimal Analysis Systems to Meet Your Needs
It is possible to con�gure a system to suit your application, including the form of the samples, and the
features and concentrations of the components.
15
Provides High-Sensitivity and FunctionalityUnder a Variety of Analytical Conditions
The GCMS-QP2020 adopts a new differential exhaust turbomolecular
pump with heightened exhaust efficiency; as a result, in addition to
helium, the system can now be operated with hydrogen or nitrogen
as the carrier gas. In addition, the system supports a variety of analysis
conditions, including high-speed scan.
(×10,000)
6.00 6.25 6.50 6.75
2.00
1.75
1.50
1.25
1.00
0.75
0.50
0.25
191.00193.00
Chloroneb
Mass chromatograms of pesticides with hydrogen carrier gas (5ppb, SIM)
Even though confirmation of molecular related ions is difficult with
the EI method, data can be acquired by Quick-CI immediately without
stopping the MS.
50 100 150 200 2500
50
100
%86
44 14912165 9141
50 100 150 200 2500
50
100
%236
86218149 18871 91 121
EI Mass Spectrum (Top) and CI Mass Spectrum (Bottom) for Pentylone, a Typeof Cathinone
Numerous Databases Support Analysis
Databases are available to satisfy a variety of needs, including
environmental analysis, foods analysis, off-flavor analysis, and forensic
analysis. All of the databases include retention indices, which support
more accurate qualitative analysis, convenient quantitative method
development, and screening analysis.
Retention Indices
SimultaneousAnalysis Database
Smart MetabolitesDatabase
Off-FlavorDatabase
Pesticide Library
FFNSC Flavor andFragrance Library
Forensic ToxicologyDatabase
Achieves High-Sensitivity and Efficiency ofMulticomponent Analysis
The GCMS Insight software package supports everything from method
creation to analysis. The Smart SIM functionality automatically creates
SIM programs that enable measurements of multiple components
with high sensitivity. In addition, a special data analysis program,
increases data processing efficiency.
Smart SIM
The optimal MS table isautomatically created
SIM method file
Data analysis program LabSolutions Insight
Configure a Range of Diverse Systems
It is possible to configure a system to suit your application, including
the form of the samples being analyzed, and the features and
concentrations of the components being measured. All the
peripherals comprising the system units are supported by Shimadzu,
so you can secure about using them.
HS-20
OPTIC-4EGA/PY-3030D AOC-6000 TD-20*
GC×GC
MDGC
* Not available in the U.S.
www.shimadzu.com/an/
Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its af�liates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.
For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.
© Shimadzu Corporation, 2015
Printed in Japan 3655-11508-30ANS16
High ReproducibilityThe HS-20 Series achieves high reproducibility through both high-accuracy flow rate
control via the pneumatic flow controller (Advanced Flow Control: AFC™ system) and
a mechanism that allows sample vial to enter the oven from the bottom.
Consequently, this system minimizes heat loss, and maintains high thermal stability
during overlap analyses.
Low CarryoverKeeping the sample line inert and as short as possible results in extremely low
carryover. No residue is left, even with acetic acid and other polar compounds,
enabling highly reliable analysis. (Patent pending)
High-Temperature CompatibilityWith an oven configurable up to 300 °C and a simple, inert
sample line, the HS-20 Series allows the analysis of
high-boiling compounds.
Cyclic siloxane is a raw silicone material, trace quantities of
which remain in oils, liquid rubber, and other products.
Because cyclic siloxane is volatile, it could potentially cause
problems with contacts in electronic parts, so controlling its
concentration is very important. The HS-20 Series makes it
possible to measure everything from cyclic siloxane to
phthalate esters under the same conditions.
Typical HS SamplerInternal heat easily escapes during vial transfer, temporarily reducing the oven temperature.
HS-20Conveyance from the bottom makes it difficult for heat to escape from inside, improving oven temperature stability. (Patent pending)
Cyclic siloxane C2nH6nOnSin (m/z 73) in resin outgas at 300 °C
Loop fillingvent line
Methanol 50 ppmreproducibility (n = 20)1.5 %
n-Butanol 50 ppmreproducibility (n = 20)1.5 %
Vial pressurizing line
Residual acetic acid in coffee< 0.0001 %
Residual DMI solvent< 0.0001 %
CoffeeBlank
DMI solventBlank
Acetic acid DMI
Sample warmed to a constant temperature of 300°C
Sample warmed to a constant temperature of 220°C
n=5 6 7 8 9 10 11 12 13 14 15 16
The HS-20 Series is the optimal solution for volatile component analysis.
Its superior performance and user-friendly design support all types of analyses, from research
to quality control.
HS-20 Series of Headspace SamplersA Revolutionary System Aimed at Performance and Ease of Use
With the trap model, concentrating the headspace gas
enables the analysis of ultra-trace components, such as
gases released from parts and materials.
The optional barcode reader enables samples to be
controlled via a chromatography data system.
Excellent Expandability
Easily place samples in the tray with the user-friendly
design. Also, the needle, sample loop, trap, and other
consumables can easily be replaced from the top of the
instrument.
User-Friendly Design
High reproducibility and low carryover ensure reliable
quantitation. In addition, an oven with a maximum
temperature of 300 °C enables analysis of high-boiling
compounds.
Excellent Performance
3
2
1
• Electronic cooling trap (trap model)• Barcode reader option
• User-friendly sample tray• Easy maintenance
• High reproducibility • Low carryover• High-temperature compatibility
Excellent Performance
7.0 8.0 9.0 10.0 11.0 12.0 min 28.0 28.25 28.5 28.7529.0029.2529.50min
HS-20 SeriesHeadspace Samplers2 318
High ReproducibilityThe HS-20 Series achieves high reproducibility through both high-accuracy flow rate
control via the pneumatic flow controller (Advanced Flow Control: AFC™ system) and
a mechanism that allows sample vial to enter the oven from the bottom.
Consequently, this system minimizes heat loss, and maintains high thermal stability
during overlap analyses.
Low CarryoverKeeping the sample line inert and as short as possible results in extremely low
carryover. No residue is left, even with acetic acid and other polar compounds,
enabling highly reliable analysis. (Patent pending)
High-Temperature CompatibilityWith an oven configurable up to 300 °C and a simple, inert
sample line, the HS-20 Series allows the analysis of
high-boiling compounds.
Cyclic siloxane is a raw silicone material, trace quantities of
which remain in oils, liquid rubber, and other products.
Because cyclic siloxane is volatile, it could potentially cause
problems with contacts in electronic parts, so controlling its
concentration is very important. The HS-20 Series makes it
possible to measure everything from cyclic siloxane to
phthalate esters under the same conditions.
Typical HS SamplerInternal heat easily escapes during vial transfer, temporarily reducing the oven temperature.
HS-20Conveyance from the bottom makes it difficult for heat to escape from inside, improving oven temperature stability. (Patent pending)
Cyclic siloxane C2nH6nOnSin (m/z 73) in resin outgas at 300 °C
Loop fillingvent line
Methanol 50 ppmreproducibility (n = 20)1.5 %
n-Butanol 50 ppmreproducibility (n = 20)1.5 %
Vial pressurizing line
Residual acetic acid in coffee< 0.0001 %
Residual DMI solvent< 0.0001 %
CoffeeBlank
DMI solventBlank
Acetic acid DMI
Sample warmed to a constant temperature of 300°C
Sample warmed to a constant temperature of 220°C
n=5 6 7 8 9 10 11 12 13 14 15 16
The HS-20 Series is the optimal solution for volatile component analysis.
Its superior performance and user-friendly design support all types of analyses, from research
to quality control.
HS-20 Series of Headspace SamplersA Revolutionary System Aimed at Performance and Ease of Use
With the trap model, concentrating the headspace gas
enables the analysis of ultra-trace components, such as
gases released from parts and materials.
The optional barcode reader enables samples to be
controlled via a chromatography data system.
Excellent Expandability
Easily place samples in the tray with the user-friendly
design. Also, the needle, sample loop, trap, and other
consumables can easily be replaced from the top of the
instrument.
User-Friendly Design
High reproducibility and low carryover ensure reliable
quantitation. In addition, an oven with a maximum
temperature of 300 °C enables analysis of high-boiling
compounds.
Excellent Performance
3
2
1
• Electronic cooling trap (trap model)• Barcode reader option
• User-friendly sample tray• Easy maintenance
• High reproducibility • Low carryover• High-temperature compatibility
Excellent Performance
7.0 8.0 9.0 10.0 11.0 12.0 min 28.0 28.25 28.5 28.7529.0029.2529.50min
HS-20 SeriesHeadspace Samplers2 319
Sample ConcentrationThe HS-20 trap model is equipped with an electronic cooling trap that concentrates the headspace gas, enabling
high-sensitivity analysis. By using hydrophobic Tenax, the trap enables the analysis of low-boiling compounds by
concentrating them to high-boiling compounds in samples containing moisture.
Method files make it easy to switch between trap and conventional modes, in which a sample loop is used. The two modes
can be combined even in continuous analysis via batch processing.
High-Sensitivity Analysis of Fragrance Components in CoffeeIn combination with a GCMS, the high-sensitivity electronic cooling trap enables qualitative and quantitative analyses of
fragrance components at trace levels undetectable with a conventional headspace sampler.
User-Friendly Sample TrayThe HS-20 sample tray is 20 cm higher than the desk,
enabling it to be seen at all times. This makes sample
placement easy.
In addition, 10 mL and 20 mL vials can be placed and
analyzed simultaneously without the need for special
attachments.
Furthermore, the optional barcode reader allows samples to
be controlled using barcodes.
Easy MaintenanceThe HS-20 Series has been designed to enable sample loop and needle
replacement and other maintenance work to be performed easily from the top of
the instrument.
Even if sample lines become contaminated by high-concentration samples, the
piping alone can now be replaced.
Also, the capillary column joints are shared with the GC sample injection unit,
enabling easy column replacement.
This superior design minimizes downtime during maintenance and improves
laboratory productivity.
Analysis of blood and other samples in the forensic field requires a system that not only offers excellent performance, but
also eliminates operational errors.
With its user-friendly design, the HS-20 Series prevents mistakes, while the barcode reader records logs to dramatically
improve traceability.
The instrument can be controlled by the CFR 21 Part 11-compliant LabSolutions chromatography data system, ensuring
traceability of analysis conditions and operations. In addition, an automatic shutdown function operates after analysis is
completed, saving electricity and carrier gas. (LabSolutions LC/GC only. Not supported by GCMS software.)
Area(x100,000)
5.5
5.0
4.5
4.0
3.5
3.0
2.5
2.0
1.5
1.0
(x1,000,000)
1.1
0 10 20 30 40 50 60 70 80 90 Concentration
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
Methylfuran
Aceticacid
Acetol
Pyridine
Methylpyrazine
Furfurylalchol
2,5 -Dimethylpirazine
Furfurylacetate
Peak areas are improved 5 to 50 times.
6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0
Electronic Cooling Trap Unit Switching Between Loop Mode and Trap Mode Using Batch Editor
1ppt TCA in Wine (Loop mode)
User-Friendly Design Excellent Expandability
Quantitative Results Browser
Area × 4S/N × 10
2,4,6-TCA 2,4,6-TCA
1ppt TCA in Wine (Trap mode) Calibration Curve from TCA Added to Wine (trap mode)
1~100pptR 0.998
HS-20Trap + GCMS-QP2010 Ultra
4 5HS-20 Series
Headspace Samplers20
Sample ConcentrationThe HS-20 trap model is equipped with an electronic cooling trap that concentrates the headspace gas, enabling
high-sensitivity analysis. By using hydrophobic Tenax, the trap enables the analysis of low-boiling compounds by
concentrating them to high-boiling compounds in samples containing moisture.
Method files make it easy to switch between trap and conventional modes, in which a sample loop is used. The two modes
can be combined even in continuous analysis via batch processing.
High-Sensitivity Analysis of Fragrance Components in CoffeeIn combination with a GCMS, the high-sensitivity electronic cooling trap enables qualitative and quantitative analyses of
fragrance components at trace levels undetectable with a conventional headspace sampler.
User-Friendly Sample TrayThe HS-20 sample tray is 20 cm higher than the desk,
enabling it to be seen at all times. This makes sample
placement easy.
In addition, 10 mL and 20 mL vials can be placed and
analyzed simultaneously without the need for special
attachments.
Furthermore, the optional barcode reader allows samples to
be controlled using barcodes.
Easy MaintenanceThe HS-20 Series has been designed to enable sample loop and needle
replacement and other maintenance work to be performed easily from the top of
the instrument.
Even if sample lines become contaminated by high-concentration samples, the
piping alone can now be replaced.
Also, the capillary column joints are shared with the GC sample injection unit,
enabling easy column replacement.
This superior design minimizes downtime during maintenance and improves
laboratory productivity.
Analysis of blood and other samples in the forensic field requires a system that not only offers excellent performance, but
also eliminates operational errors.
With its user-friendly design, the HS-20 Series prevents mistakes, while the barcode reader records logs to dramatically
improve traceability.
The instrument can be controlled by the CFR 21 Part 11-compliant LabSolutions chromatography data system, ensuring
traceability of analysis conditions and operations. In addition, an automatic shutdown function operates after analysis is
completed, saving electricity and carrier gas. (LabSolutions LC/GC only. Not supported by GCMS software.)
Area(x100,000)
5.5
5.0
4.5
4.0
3.5
3.0
2.5
2.0
1.5
1.0
(x1,000,000)
1.1
0 10 20 30 40 50 60 70 80 90 Concentration
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
Methylfuran
Aceticacid
Acetol
Pyridine
Methylpyrazine
Furfurylalchol
2,5 -Dimethylpirazine
Furfurylacetate
Peak areas are improved 5 to 50 times.
6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0
Electronic Cooling Trap Unit Switching Between Loop Mode and Trap Mode Using Batch Editor
1ppt TCA in Wine (Loop mode)
User-Friendly Design Excellent Expandability
Quantitative Results Browser
Area × 4S/N × 10
2,4,6-TCA 2,4,6-TCA
1ppt TCA in Wine (Trap mode) Calibration Curve from TCA Added to Wine (trap mode)
1~100pptR 0.998
HS-20Trap + GCMS-QP2010 Ultra
4 5HS-20 Series
Headspace Samplers21
Batch Analysis of VOCs in WastewaterWith its high thermal stability and inert sample line, the HS-20 Series can measure VOCs in wastewater with high
reproducibility. Carryover is minimal, so the sample tray, which is capable of holding 90 samples, can be effectively
utilized.
Aqueous Solution of USP467 Class 2A/2B Pharmaceutical Residual SolventsIn combination with a robust GC detector, the system can be used for quality control requiring high quantitative
accuracy. Traceability is guaranteed by the all-sample leak check function and the digitized log function.
4.0
min
5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0
1
2
3 4 5
6 7 8
9
10 11
12 13
14
15
17 16
18
19
20
21
22
2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0 32.5 35.0
min2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0 32.5 35.0
Sample Injection Method
Number of Vials
Vials
Vial Mixing
Leak Check
Optional Barcode Reader
Vial Warming Temperature
Sample Line Temperature
Transfer Line Temperature
Trap
Trap Cooling Temperature
Trap Heating Temperature
Carrier Gas Control
Vial Pressurized-Gas Control
Carrier Gas and Vial Pressurizing Gas
PC Interface
Control Software
Software Operating Environment
Guaranteed Operating Environment
Power Supply
Dimensions
Weight
Sulfinert sample loop 1 mL (standard); 0.2 mL, 3 mL (optional) or trap (HS-20Trap)
90
Outer dia. 22.5 mm × 79 mm (20 mL); outer dia. 22.5 mm × 46 mm (10 mL); can be combined
5 stages max.
All-vial automatic check
Optional, can read 6 types of barcodes
Room temperature + 10 to 300 °C (Settings are 0 to 300 °C, in 1 °C units, with an accuracy of ±0.1 °C)
Room temperature + 10 to 220 °C or 150 to 300 °C (set in 1 °C units, accuracy of ±0.5 °C)
Long transfer line model (HS-20LT): Room temperature + 10 to 220 °C
Room temperature + 10 to 350 °C (set in 1 °C units, accuracy of ±0.5 °C)
Long transfer line model (HS-20LT): Room temperature + 10 to 200 °C
Inner dia. 2 mm × 100 mm, Sulfinert tube
Filler TenaxTA (standard), Carbopack + Carboxene (optional)
-30 to 80 °C (set in 1 °C units, accuracy of ±1 °C)
For a sample line at 250 to 300 °C, room temperature - 30 °C
For 150 to 250 °C, room temperature - 40 °C
For 150 °C or less, room temperature - 50 °C
0 to 350 °C (set in 1 °C units, accuracy of ±1 °C)
Electronic control via AFC built into GC
Electronic control via APC built into GC
High-purity helium or nitrogen
USB
Operates collectively with LabSolutions LC/GC (FDA CFR 21 Part 11 compliant)
HSS Control Software is used for GCMS.
Windows XP, Windows Vista, Windows 7 (32/64-bit)
15 to 30 °C, humidity up to 70 % RH (performance guaranteed at 18 to 28 °C with temperature
fluctuations within ±1.3 °C)
1200 VA max. (HS-20, HS-20LT), 1500 VA max. (HS-20Trap)
W553 mm × H430 mm × D543 mm, excluding PC
33 kg (HS-20, HS-20LT), 40 kg (HS-20Trap)
1. 1,1-Dichloroethene 2 Dichloromethane
3 trans-1,2-Dichloroethene
4 cis-1,2-Dichloroethene5 Chloroform
6 1,1,1-Trichloroethane7 Carbon tetrachloride
8 1,2-Dichloroethane9 Benzene
10 Trichloroethene
11 1,2-Dichloropropane
12 Bromochloroethane
13 cis-1,3-Dichloropropane
14 Toluene 15 trans-1,3-Dichloropropane
16 1,1,2-Trichloroethane17 Tetrachloroethene
18 Dibromochloromethane
19 m+p-Xylene20 o-Xylene
21 Bromoform22 1,4-Dichlorobenzene
1.83.0
1.4
2.82.3
1.72.2
2.70.7
1.2
3.1
2.0
1.8
1.41.8
2.90.8
2.1
1.71.4
2.31.2
Reproducibility of USP467 Class 2A/2B Procedure A(Aqueous Solution) RSD% (n=20) 2 Acetnitrile 1.1
3 Dichloromethane 1.7
4 trans-1,2-Dichloroethene 2.3 5 cis-1,2-Dichloroethene 1.9
6 THF 0.6 10 Toluene 2.5
11 Chlorobenzene 2.5
18 1,2-Dimethoxyethane 3.1
20 Pyridine 2.6
Transfer line
HS-20Model
VOC 0.1 ppb reproducibility RSD% (n = 5)
HS-20LTHS-20Trap
Trap NoNo Yes
Long (200 °C)Short (350 °C)Short (350 °C)
HS-20 + GC-2010 Plus
HS-20 HS-20Trap19
17
18
16
15
14
13
12
11
10
20
8 +9
7
6
5 4
3
2
1
IS IS
21
22
6 7HS-20 Series
Headspace Samplers
1418
440
543
Dimensions (HS-20Trap + GCMS-QP2010 Ultra) units: mm
Specifications and Installation Conditions
22