Shannon Marie Johnson863-B Woodpoint DriveChesterfield, MO 63017

Mobil Phone: 314-306-8960Email: shannon.marie.johnson@monsanto.com

________________________________________________________________________________________ EducationB.S., Major: Biology, Minor: Chemistry, University of Wisconsin - Eau ClaireCertification: Broad Field Science, University of Wisconsin - Eau Claire________________________________________________________________________________________ Recent ExperienceMonsanto, Chesterfield Village Campus, Automation Engineer, Chesterfield, MO▪          Member of the Systems Reliability Team (SRT) performing maintenance and repairs for the Chesterfield Village Research Laboratories.▪          Program and develop Biomek FX methods to increase throughput, efficiencies, and reduce costs for Molecular Breeding Techologies.▪          Member of the STL Biotechnology ISO 9001:2008 – Internal Auditor, QMS Champion▪          Participating in Biotech Lean Practitioner Training.▪          Utilize Spotfire Visualization tool for Maintenance Connection for analysis of work orders, technicians, types of repairs and equipment locations.▪          Participate on the team implementing Splunk to track equipment processes, down time, error logs, and frequency of equipment failures.

Monsanto, Agracetus Campus, Automation Engineer, Middleton, WI▪          Strong background with various liquid handlers including Bravos, PlateMates, WellMates, Tecan Genesis, Biomek FX, Biomek NX, Hamilton StarPlus, and Hamilton Starlet. Familiar with other automation friendly devices including Agilent BenchCells, Agilent VSpin, Agilent VCode, Liconic Incubator, Thermo Scientific Incubator, Sigma 6-KR centrifuge and Porvair Ultravaps.▪          Experienced in multiple software languages including BenchWorks, VWorks 3.0, VWorks 4.0, Venus One, Biomek FX, and SAMI EX▪          Experienced in programming various automated platforms including TECAN, Biomek FX, Biomek NX, Agilent, Hamilton, and other bench top liquid handlers. Successfully developed, validated, and implemented multiple automated assays in 96, and 384 well automated formats. Maintain the instrumentation according to SOP, GLP, and QC requirements.▪          Basic understanding of agricultural genetics including DNA extraction, invader assay technology, and data analysis. ▪          Assessed and recommended new automation to meet or exceed current processes, within a stringent timeframe. Purchased approximately 1.5 million capital items within 6 months of hire. Coordinated the installation and site preparation for new automation, including assessing space and workflow concerns.▪          Implemented new custom automation for Mega Soybean Chippers with partnering St. Louis colleagues. Support and maintain Mega Soybean Chippers automation, troubleshooting and maintenance.▪          Train multiple colleagues on troubleshooting, programming, error recovery and development of automated assays on multiple automated platforms.▪          Molecular Breeding Quality and Characterization (MBQC) Quality Management System Lab Champion for ISO9001:2008.▪          Trained as an ISO9001:2008 Internal Quality Auditor for International Quality Management Systems. Perform Internal audits with Lead auditor.

▪          Member of the Middleton Equipment Maintenance Team involving Maintenance Connection work orders and preventative maintenance. Matrix report to Molecular Breeding Technology (MBT) - Global Systems Reliability Team Lead - Kevin Desrosiers and participate in quarterly Monsanto Maintenance Team meetings.

▪          Recipient of Monsanto Commitment to Excellence Award in 2012 and 2013.

BioDiagnostics, Inc., Laboratory Assistant, River Falls, WI ▪          Perform various seed testing (ELISA, Herbicide Bio-Assay), primarily on corn and soybean crops.▪          Basic knowledge on qualitative trait testing (Cry1AB, Cry1F, Cry3Bb, Cry2Ab, CP4, Cry34) on corn crops using ELISA methods, SpectraMax, and Biomek FX automation.▪          Experience with planting, harvesting and evaluation of corn and soybean crops, for ELISA and Herbicide (Liberty and Roundup Ready) studies.▪          Basic understanding on Standard Seed Testing (SST), for cold/warm seed viability/evaluation/planting.▪          Basic understanding on Breeder studies (ELISA, Herbicide Bio-Assay), including planting, and evaluation. Pfizer Global Research & Development, Scientist, La Jolla, CA▪          Successfully developed, validated, and implemented multiple in-vitro ADME assays in 24, 96, and 384 well automated formats, including caco-2, porcine bovine microvessel endothiel cells (PBMEC), blood brain barrier transporters (hTAUT1, hSMVT1, hCT1, hCAT1 and hLAT1), liver uptake transporters (OATP-8, OATP-B, OATP-C), kidney transporter (OAT1), intestinal efflux MDCK-MDR1, ELISA and metabolism studies (rat and human microsomes, rat,dog and human hepatocytes).▪          Strong background in the development and validation of transporter science including blood-brain barrier, liver, kidney, and intestinal transporters.▪          Experienced in cell culture techniques for multiple in-vitro cell lines (caco-2, MDCK-MDR1, PBMEC, OATP-8, OATP-B, OATP-C, hTAUT1, hSMVT1, hCT1, hCAT1, hLAT1, HEK wild type).▪          Experienced in programming various automated platforms including TECAN, Biomek FX, Biomek NX and other bench top liquid handlers. Maintained the instrumentation according to SOP, GLP, and QC requirements.▪          Basic understanding of biotherapeutics including vaccines, monoclonal antibodies, western blot, immunoassay and flow cytometry.▪          Participated on IT related teams developing internal software applications for data analysis, data storage, and data retrieval with 21 CFR 11 compliance.▪          Participated on global teams for in-vitro ADME related studies to discuss assay performance, utility, QC guidelines, and data interpretation guidelines.▪          Knowledgeable with computational ADME science, SAR, in-silico modeling, and Spotfire visualization tool.▪          Prepared IV/PO dose formulations for PK/PD pre-clinical rat and dog in-vivo studies. Performed PK/PD pre-clinical in-vivo dog studies, including IV/PO dosing and intravenous sample collection.▪          Experienced with computers including extensive knowledge of Microsoft: Excel, Access, PowerPoint, Word, and Visual Basic. Strong IT background with internal applications including RGate (nominated as RGate Champion), IVAD, GeCA3.0, Screening Services, Pipeline Pilot.▪          Trained multiple scientists in cell culture techniques, automation execution (calibrations, trouble shooting), in-vitro ADME data analysis and QC, and internal applications. Supervisory Experience▪          Coordinated multiple production assay scheduling/execution, data analysis, QC, and cycle times with 98% on-time data delivery, according to GLP and SOP guidelines▪          Directly supervised one direct report, and indirectly supervised 11 scientists responsible for the in-vitro ADME production assay execution. Supervised a multiple user cell culture laboratory with

approximately 8 users to adhere to SOP’s, GLP, ordering/stocking supplies, scheduling PM’s and calibration of equipment.▪          Trained and developed multiple colleagues with in-vitro ADME assay execution, including instrumentation, data analysis, data reporting, and trouble shooting.▪          Ability to successfully build strong partnerships across all levels of the organization, including other sections, departments, and sites. ▪          Maintained strong customer focus with chemists, including providing detailed data interpretation, educating the use of RGate application, educating assay modifications/implications, and created a brochure outlining these customer needs.▪          Ability to sustain focus on the business needs during times of change, including multiple mergers and acquisitions, and organizational restructuring.▪          Ability to effectively reduce resources, increase throughput by 33% while still maintaining quality data generation and cycle times.▪          Partnered with Research Informatics to develop two in-house software applications for data storage, data analysis, and QC flags.▪          Partnered with Non-Clinical Biostatistics to statistically create QC upper and lower limits, which was implemented, and was the first statistically driven QC implementation across the globe.▪          Ability to evaluate new technologies and make recommendations for implementation to senior management based on the utility, cost effectiveness, and resource needs.▪          Completed numerous management courses, including the Essentials for the Pfizer Manager, instructed by the University of Michigan - School of Business.▪          Successfully refined and implemented new processes related to high-throughput screening.________________________________________________________________________________________ Employment History Monsanto, Agracetus Campus, Middleton , WI2011 -Present▪          Automated, programmed, developed, implemented and validated new assays.▪          Maintain operation and processes related to production operation, while trying to maintain cycle times. ▪          Trained colleagues on programming and troubleshooting, on multiple automated platforms.▪          Implemented, and partnered with other contractors and colleagues for site installation on two new systems.▪          Coordinated and partnered with Beckman Coulter Sales, Field Engineers, Project Field Managers in the purchase, design, and installation of our Biomek NX Integrated system and our dual multichannel Biomek FX BioDiagnostics, Inc., Laboratory Assistant, River Falls, WI 2010 - 2011▪          Perform various seeding testing (ELISA, Herbicide Bio-Assay), primarily on corn and soybean crops.▪          Basic knowledge on qualitative trait testing (Cry1AB, Cry1F, Cry3Bb, Cry2Ab, CP4, Cry34) on corn crops using ELISA methods, SpectraMax, and Biomek FX automation.▪          Experience with planting, harvesting and evaluation of corn and soybean crops, for ELISA and Herbicide (Liberty and Roundup Ready) studies.▪          Basic understanding on Standard Seed Testing (SST), for cold/warm seed viability/evaluation/planting.▪          Basic understanding on breeder studies (ELISA, Herbicide Bio-Assay), including planting, and evaluation. Pfizer Global Research & Development, Scientist, La Jolla, CA2000 - 2009

▪          Provided support for ADME related studies to support CNS and Oncology drug discovery projects.▪          Automated, programmed, developed, implemented and validated new assays.▪          Supervised ADME assay execution, including scheduling, cycle-times, and QC.▪          Participated on IT related teams to develop new in-house software to improve data handling. Eli Lilly (Kelly Scientific), Senior Laboratory Technician, Indianapolis, IN1997 - 1999▪          Performed automated caco-2 studies, and HPLC method development, according to GMP and SOP.▪          Programmed using Visual Basic and Microsoft Access for data handling and storage. Indiana University School of Medicine, Research Technician, Indianapolis, IN1997▪          Analyzed various isotopes (C13, O18, and H2) using IRMS instrumentation and performed combustion experiments with cholesterol and triglycerides to study energy expenditure and lipid metabolism. Trostel, Ltd., Polymer Chemist, Lake Geneva, WI1994-1996▪          Practical knowledge in the research and development of polymer-based rubber compounds.▪          Evaluated alternative raw chemical ingredients as potential replacements to reduce material costs and improve material processing.▪          Participated on cross-functional teams to reduce product to reduce product defects, and manufacturing loss. Performed failure analysis on part failures.▪          Conducted physical testing on polymer-based rubber compounds using rheometers, tensiometers, mooney and gehman instrumentation, to support production.▪          Monitored and reviewed QC for production approval of compound batches for molding readiness.▪          Created, revised SOP’s and DOP’s for QS 9000 certification.________________________________________________________________________________________Professional Affiliations American Association of Pharmaceutical Scientists (AAPS), MemberAssociation for Laboratory Automation (ALA), Member________________________________________________________________________________________ Publications/PresentationsPorcine brain microvessel endothelial cells as an in vitro model to predict in vivo blood-brain barrier permeability. Zhang Y, Ye Y, Johnson K, Poe J, Johnson S, Bobrowski W, Garrido R, Madhu C. Drug Metab Dispos. 2006 Nov; 34(11):1935-43. Epub 2006 Aug 8. “Automation of a high-throughput P-glycoprotein inhibition screen”. Shannon Johnson1, Shamsi Raeissi1, Mary Beth Dunklee1, Chad Stoner1, Dongyang He2, Chakrapani Koneru2, Kjell Johnson3, Fareena Mutlib1, Doug Burdette1, Jasminder Sahi1, and Narayanan Surendran1, 1Pharmacokinetics, Dynamics, and Metabolism; 2Development Sciences Informatics; 3Nonclinical Biostatistics, Pfizer Global Research & Development, Ann Arbor, MI. “Strategy and implementation of harmonized HT caco-2 assay in AA PDM”. Shannon Johnson1, Kjell Johnson3, Doug Burdette1, Sascha Freiwald1, Laura Hayter1, Danielle Smith1, Nicholas Paschka1, Mary Beth Dunklee1, Dongyang He2, Howard Miller1, Janet Gagnon-Yerkie1, and Narayanan Surendran1, 1Pharmacokinetics, Dynamics, and Metabolism; 2Development Sciences Informatics; 3Nonclinical Biostatistics, Pfizer Global Research & Development, Ann Arbor, MI.