Sex, Stress, and Oxygen Deprivation: Gender-Specific Phenotypes Modulate Survival in Anoxia
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Sex, Stress, and Oxygen Deprivation: Gender-Specific Phenotypes Modulate
Survival in Anoxia Michelle LeBlanc
University of North TexasRonald E. McNair Program
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Oxygen Deprivation
• What is Anoxia?– <.001 kilopascal of oxygen in environment– Health Issues
• Stroke• Ischemia• Center of cancerous tumors
– Environmental Deficits• Oceanic dead zones
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Suspended Animation
• Reversible state of dormancy – initiated due to environmental stress
• Mammals– Hibernation– Estivation
• We can study SA with the model organism Caenorhabditis elegans!!
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Caenorhabditis elegans
Image adapted from Wormbook
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Image Adapted from Wormbook
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Hypothesis
• An modification in gonad function will modulate survival of long-term oxygen deprivation.– Sterile animals may survive anoxia differently
than wild-type animals.– Sex may modulate survival in anoxia.
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Experimental Schematic
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RNAi of sterile-reported genes
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Gender in Anoxia
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Male Genotype vs Phenotype
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Females in anoxia
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Oocyte flux and survival
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Future Aims
• What underlies the fog-2(q71) phenotype?– Sperm signaling– Signaling from fertilized embryo
• Does oocyte flux affect survival?– spe-12(hc76) and fer-15(hc15)
• Animals that cannot produce spermatids
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Acknowledgements
Ronald E. McNair Program
Dr. Pamela PadillaAlexander Mendenhall and Desh
Mohan, project collaboratorsThe rest of the Padilla lab
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