Serial EM 3D Electron microscopy (serial sections) of the brain Large datasets 10-100 terabytes High...

5
40,000x40,000 pixels 1.6 GB 120x120 m (3 nm/pixel) Here shown 40x undersampled or 1/1600 th of the data in a single section. he goal, 10,000 such sections making up a 3D volume Reconstruct the big axons first: the “wires” Then find the big synapses: the connections Then zoom in 6 15mu EM big view (Davi Bock and Clay Reid)

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Transcript of Serial EM 3D Electron microscopy (serial sections) of the brain Large datasets 10-100 terabytes High...

Page 1: Serial EM 3D Electron microscopy (serial sections) of the brain Large datasets 10-100 terabytes High resolution: ~5 nm X-Y, 50 nm Z (10 5 x10 5x 10 4 x.

40,000x40,000 pixels1.6 GB

120x120 m (3 nm/pixel)

Here shown 40x undersampledor 1/1600th of the data in a single section.

The goal, 10,000 such sections making up a 3D volume

Reconstruct thebig axons first:

the “wires”

Then find the bigsynapses:

the connections

Then zoom in

6 15mu EM big view

(Davi Bock and Clay Reid)

Page 2: Serial EM 3D Electron microscopy (serial sections) of the brain Large datasets 10-100 terabytes High resolution: ~5 nm X-Y, 50 nm Z (10 5 x10 5x 10 4 x.

5 8mu rlp

Andzoom in

Find thepresynapticterminals

Page 3: Serial EM 3D Electron microscopy (serial sections) of the brain Large datasets 10-100 terabytes High resolution: ~5 nm X-Y, 50 nm Z (10 5 x10 5x 10 4 x.

4 3mu rlp

Andzoom in

Page 4: Serial EM 3D Electron microscopy (serial sections) of the brain Large datasets 10-100 terabytes High resolution: ~5 nm X-Y, 50 nm Z (10 5 x10 5x 10 4 x.

3 1mu rlp

Andzoom in

Page 5: Serial EM 3D Electron microscopy (serial sections) of the brain Large datasets 10-100 terabytes High resolution: ~5 nm X-Y, 50 nm Z (10 5 x10 5x 10 4 x.

2 300 nm rlp

Until you identifya synapse

(one connection out of many thousand in the circuit)

Zoomed in ~40x, or 1/1600th of the total field of view.

Displayed at native resolution.


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