Fluorescence and Biology

Sergi Padilla

George Gabriel Stokes

Pink Floyd?microscope

Gin Tonic and Stokes Experiment

U.V Light!

Quinine found in tonic water!

FRET:

FRET and Lifetime:

Jelly fish glowing in the dark

Biological light?

Green Fluorescent Protein out of Aquorea Victoria

Fluorescence decay: A population of a bunch of molecules relaxing

∆t

τD

τDA

Fluorescence decay of the donor

FRETefficiency

∆t

τD

donor

Proportionbound donor

log Ilog I

Measurement of the mean lifetime:

•.ACQUISITION BEFORE BLEACHING

•. ACQUISITION AFTER BLEACHING

A Proof of FRET by FLIM

+ +

The Nobel prize in Chemistry 2008