Sequence announcement

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Plant Molecular Biology 38: 909, 1998. © 1998 Kluwer Academic Publishers. Printed in the Netherlands. 909 Sequence announcement EMBL accession #: AJ224109 Authors: Chiang TY 1 , Hsing YIC 2 , Hsieh JS 3 Addresses 1 : Department of Biology, Cheng-Kung University, Tainan, Taiwan 700; 2 Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 115; 3 Department of Agronomy, Taiwan University, Taipei, Taiwan Source of sequence: Sequence was determined from a PCR product, which was ligated to a T-vector, from genomic DNA of Glycine soja Sieb. & Zucc., a wild soybean (isolate 043) collected from South China. Trivial name: rDNA ITS region of wild soybean Description: ITS region of ribosomal DNA lies between 18S and 28S rRNA genes was cloned from wild soybean, Glycine soja, with 216 bp in ITS1, 169 bp in 5.8S rRNA gene and 211 bp in ITS2. Nucleotide sequence of ITS region in Glycine soja shows 98.8% and 88.2% identity with cultivated soybean, Glycine max L., and Glycine falcata Benth. respectively. Acknowledgements. This research was supported by a grant from Academia Sinica, Taiwan, ROC, to Y.I.C.H. Figure 1. Nucleotide sequence of ITS region of rDNA from Glycine soja.

Transcript of Sequence announcement

Plant Molecular Biology38: 909, 1998.© 1998Kluwer Academic Publishers. Printed in the Netherlands.

909

Sequence announcement

• EMBL accession #:AJ224109Authors: Chiang TY1, Hsing YIC2, Hsieh JS3

Addresses1: Department of Biology, Cheng-Kung University, Tainan, Taiwan 700;2Institute of Botany,Academia Sinica, Nankang, Taipei, Taiwan 115;3Department of Agronomy, Taiwan University, Taipei,Taiwan

Source of sequence:Sequence was determined from a PCR product, which was ligated to a T-vector, fromgenomic DNA ofGlycine sojaSieb. & Zucc., a wild soybean (isolate 043) collected from South China.

Trivial name: rDNA ITS region of wild soybeanDescription: ITS region of ribosomal DNA lies between 18S and 28S rRNA genes was cloned from

wild soybean,Glycine soja, with 216 bp in ITS1, 169 bp in 5.8S rRNA gene and 211 bp in ITS2.Nucleotide sequence of ITS region inGlycine sojashows 98.8% and 88.2% identity with cultivatedsoybean,Glycine maxL., andGlycine falcataBenth. respectively.

Acknowledgements.This research was supported by a grant from Academia Sinica, Taiwan, ROC, toY.I.C.H.

Figure 1. Nucleotide sequence of ITS region of rDNA fromGlycine soja.