SEPARATION OF BLOOD PROTEINS - MIRINZ
Transcript of SEPARATION OF BLOOD PROTEINS - MIRINZ
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SEPARATION OF BLOOD PROTEINS
Dr Derek KnightonBioProcessing GroupDairy Science and Technology
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Abattoir blood collection
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Alfa Laval Blood Separator
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Blood separation into plasma and red blood cells
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Plasma and Red Blood Cells
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Plasma Protein Separation Methods
• Cohn fractionation
• Modified Cohn fractionation (Ammonium sulphate)
• Heat shock
• Chromatography
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Cohn Fractionation
• pH, salts and ethanol concentration controlled
• 5 step process
• Special flame-proof facilities
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Modified Cohn Process -Ammonium Sulphate Fractionation
• Possible to separate major proteins
• pH
• Ammonium Sulphate
• Centrifugal separation of precipitate
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Heat shock method
• Caprylate 1:1
• pH controlled
• Heat (67°C 4h)
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Chromatography method
• Multistep
• Expensive
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Pilot scale
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Heat Shock Fractionation and Modified Cohn Fractionation
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Ultrafiltration of ammonium sulphate and heat shock fractions
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Pilot Scale Ultrafiltration
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Freeze-drying
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SDS Page Electrophoresis
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Blood Proteins on SDS Page Gel Electrophoresis
Lanes
1. Bovine Plasma
2. IgG
3. Fibrinogen
4. Albumin (Sigma)
5. Transferrin
6. Haemoglobin
1 2 3 4 5 6
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Agarose Gel of Blood Fractionation
IgG
FIBRINOGEN
TRANSFERRIN
ALBUMIN
PLASMA STDS
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Other Co-products Expertise
• Blood products (deer, sheep & beef)
• Various organ extracts
• Aprotinin from bovine lung (analytical, process)
• Pancreas (analytical, process)
• Intestine (sheep & beef)
• Collagen (corium, extraction, denaturation)
• GAGs (stick water, connective tissue)
• Meat & bone meal quality (analytical and processing)
• Bone (prosthesis, photography grade gelatin, hydroxy-apatite, marrow
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BioProcessing Group Expertise
• Meat co-products
• Dairy products
• Hydrolysis of food proteins
• Fermentation
• Protein expression