Seminar and Workshop: Environmental Prevention, Monitoring ...
Transcript of Seminar and Workshop: Environmental Prevention, Monitoring ...
How to Resolve Unresolved Complex Mixture
Vladimir P. BeškoskiDepartment of Biochemistry, Faculty of Chemistry
University of Belgrade, Belgrade, Serbia [email protected]
Institut Vinča20.11.2018.
Seminar and Workshop: Environmental Protection
Prevention, Monitoring and Remediation -Worldwide and Our Experiences
ENVIRONMENT IN SERBIA
Europe ~ 1,800,000 potentially contaminated sites/240,000 sites require remediation!
Official data from Serbia - 357 heavy polluted sites!
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Locations of known illegal dumps in the territory of the Republic of Serbia, REPORT ON THE STATE OF THE ENVIRONMENT IN SERBIA 2009,
Ministry of Environment and Spatial Planning
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THE ESSENCE OF BIOREMEDIATION
“BIOLOGICAL AGENT”
MICROORGANISMS
HARMFUL SUBSTANCES
SUBSTANCES WITHOUT
HARMFUL EFFECT ON THE
ENVIRONMENT AND HUMANS
+ BIOMASS
GREEN BIO/TECHNOLOGY!!!
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FROM LABORATORY…
…VIA PILOT…150m
3of soil in “Oil Refinery
Pančevo”
TO INDUSTRIAL SCALE
BIOREMEDIATION
600 m3
of soil, JKP BE
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BIOREMEDIATION FROM LABORATORY TO INDUSTRIAL SCALE
Methods
BiochemicalMicrobiologicalChemical
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No. SampleTPH (g/kg) Reduction
of TPH (%)0 days 120 days
1. 3NST8
103,2
48,1 53
2. 3NST8-SG* 38,5 63
3. 3NST8-BS* 14,3 86
4. 1NS Sludge
412,5
221,7 46
5. 1NS Sludge-SG 88,8 79
6. 1NS Sludge-BS 47,0 89
Reduction of Total Petroleum Hydrocarbons (TPH) in experiments on the laboratory shaker (Erlenmeyer flasks) - gravimetric method
The degradation rate was from 1,8 – 6,4 g/kg/day.*Surface active substances
LABORATORY LEVEL
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0
1
2
3
4
5
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7
8
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0 20 40 60 80 100 120 140
log
No
. of
mic
roo
rgan
ism
s (C
FU/g
)
TIME (days)
TC
ANA
HD
YM
Ps.
Bac.
LABORATORY LEVEL
BASIC MICROBIOLOGICAL DATA
TC-Total Chemoorganoheterotrophs; ANA – Anaerobic microorganisms; HD –Hydrocarbon degraders; YM – Yeast and Molds; Ps. – Pseudomonas spp.;
Bac. – Bacillus spp.
Milic et al., J Serb Chem Soc 2009;74:455-60
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Jovančićević et al., Water Air and Soil Pollution 2008; 190:299-307
Graphical representation of the dynamics of pilot
bioremediation in the function of time
~30 g/kg
2,2 g/kg
(~93%)
173 mg/kg/day
292 mg/kg/day
64 mg/kg/day
0-60 days
60-90 days
90-180 days
21,5
7,8
Total:151 mg/kg/day
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Comparative gas chromatograms of TPH at the beginning (Start) and after the completed bioremediation (End) at the pilot biopile
Start End(180 days)
Miletić et al., CLEAN – Soil, Air, Water 2014;42:1280–83
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Reduction of TPH
5,2
2,1
1,3
0,3
Average rate of biodegradation0-50 days: 62 mg/kg/day50-100 days: 16 mg/kg/day100-150 days: 20 mg/kg/day
GAS CHROMATOGRAPHY IN BIOREMEDIATION
Beškoski et al., Chemosphere 2011;83:34-40
GC-FID
UCMUnresolved
ComplexMixture
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Beškoski et al., J Serb Chem Soc 2010;75:1605–16
Fragmentograms of the isoprenoids (SIM, m/z 183) of the alkane fractions in the samples S-0 and S-150
GC-MS analysis - SIM mode
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Fragmentograms of terpanes (SIM, m/z 191) of alkane fractions in samples S-0 and S-150.
Fragmentograms of steranes (SIM, m/z 217) of alkane fractions in samples S-0 and S-
150.
GC-MS analysis - SIM mode
Novaković et al., Environ Chem Lett 2012;10:287–94
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Comprehensive GCxGC-MS analysisFaculty of Chemistry, University of Belgrade
Comprehensive GCxGC-MS analysis
UCM finally
resolved!
BIOCHEMICAL PROFILING OF MICROORGANISMS USING COMPREHENSIVE GCxGC-MS
Djurić et al., Int J Biol Macromol 2017;104:311-21.Izrael-Živković et al., Journal of Molecular Catalysis. B, Enzymatic 2017; in press
The fatty acid methyl ester profile of
Brachybacterium sp. CH-KOV3 a) GC–MS;
b) 2D GCxGC–MS.
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Lama et al., ACS Sustainable Chemistry & Engineering 2017;5:2415-20Beškoski et al., CLEAN – Soil, Air, Water 2017;45:1600023Kannan et al., Environ Sci Pollut R 2015, in press
•TPH (>1000)•PAHs (>16) •PCBs (209)•Pesticides (>100)•Lignin•Metabolite profiling (metabolomics)•Biodegradation products – non targeted analysis•Natural products (plant extracts, propolis, honey…)•…
Comprehensive GCxGC-MS posibilities
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Thank you for your attention