27

Roberto AVOLAFull Professor

Daniele F. CONDORELLIFull Professor

Gabriella LUPOAssociate Professor

Giovanni LI VOLTIFull Professor

Maria Teresa CAMBRIAAssistant Professor

Maria VIOLAAssistant Professor

Daniele TIBULLOAssistant Professor

Vittoria SPINA PURRELLO Assistant Professor

Vincenzo G. NICOLETTIAssociate Professor

C. Daniela ANFUSOAssociate Professor

Angela M. AMORINIAssociate Professor

Giuseppe LAZZARINOFull ProfessorSection Coordinator

Biochemistry

Biochemistry

Faculty members

Section of Medical Biochemistry

Medical Genetics

Human Nutrition and DieteticsClinical Biochemistry and Clinical Molecular Biology

Vincenza BARRESIAssociate Professor

Angela TROVATO SALINAROAssistant Professor

Vittorio CALABRESEFull Professor

Teresa MATTINAAssociate Professor

Fabio GALVANOFull Professor

Giuseppe GROSSOAssistant Professor

Marco FICHERAAssociate Professor

Biometec: a research for life

28 Biometec: a research for life

Section of Medical BiochemistryNetwork of research lines

Biomolecular role ofproteins belonging to

the family ofpoly (ADP-ribose)

polymerases(PARPs).

Evaluaon of non-canonicalfuncons of heme

oxygenases inhematological malignancies

Biochemicalpatways andcancer driver

genes

Role of Dietand foods

in the prevenon ofcancer

Neurohormesis,Redox proteomicsand Lipidomics in

Aging andNeurodegenerave

Disorders

Mitochondrial dysfuncon,energy metabolism and

oxidave/nitrosave stress inacute and chronic

neurodegeneraons

Biochemical properes of alpha Lipoicacid in neurodegenerave and

oncological models: in vitro, ex vivo andin vivo zebrafish-based approaches

Angiogenesis in cancerand blood brain/blood

renal barriers

Genec high-riskfactors and modifier

genes inneurodevelopmentaland neuropsychiatric

disorders

Magnec nanoparcles anddrug delivery

BRAINAND

RETINA

CANCER

NUTRITION

Molecular basis ofneurodegenerave diseases

correlated to protein mysfolding:metallostasis, neurotrophins,

UPS system

29Biometec: a research for life

Section of Medical Biochemistry

J Cell Biochem. 2018

Scientific Report 2018 Oct 4;8(1):14826.

30 Biometec: a research for life

Section of Medical Biochemistry

31Biometec: a research for life

Section of Medical Biochemistry Tumor Angiogenesis

Signaling pathway of angiogenesisSTUDY FOR THE MOLECULAR MECHANISM

In vitro Angiogenesis - tube formation

BLOOD BRAIN BARRIER AND BLOOD RETINAL BARRIER

Section of Medical BiochemistryFunding AgenciesRicerca corrente Min. Sal. Analisi whole exome sequencing in pazienti affetti da disordini del neurosviluppo e caratterizzati da alti livelli di omozigosità.

Ricerca finalizzata Min. Sal. To unravel the molecular basis of neurodevelopmental disorders.

Programma Operativo Nazionale Ricerca e Competitività.Identificazione di biomarcatori e sviluppo di metodi diagnostici e terapeutici nel campo dell’oncologia e della biologia vascolare.

“FIR 2014”. Classificazione di carcinomi del colon con Instabilità dei Microsatelliti: analisi massiva e parallela per l’identificazione di mutazioni somatiche ed aberrazioni cromosomiche

Progetto europeo PHAR-IN: Lifelong Learning Programme - Competences for industrial pharmacy practice in biotechnology

PO.FERS - Sicilia 2007/2013 - linea 4.1.2.A (2013-2015) “Piattaforma regionale di ricerca traslazionale per la salute”

Bando 2016 “Roche per la Ricerca” Studio di nuovi marcatori molecolari e radiologici nella diagnosi e prognosi della Fibrosi Polmonare Idiopatica

Cancer Genetics 210 (2017) 9–21

www.fir2014.unict.it/UNIVERSITÀ DI CATANIA

Biometec: a research for life32

Section of Medical Biochemistry

Research paper selection of Biometec (2014-2018)Multifunctional magnetic nanoparticles for enhanced intracellular drug transport.Tudisco C, Cambria MT, Sinatra F, Bertani F, Alba A, Giuffrida AE, Saccone S, Fantechi E, Innocenti C, Sangregorio C, Dalcanale E, Condorelli GG. Journal of Materials Chemistry B 2015 3 (20), 4134-4145.

PJ-34 inhibits PARP-1 expression and ERK phosphorylation in glioma-conditioned brain microvascular endothelial cells.Motta C, D’Angeli F, Scalia M, Satriano C, Barbagallo D, Naletova I, Anfuso CD, Lupo G, Spina-Purrello V. Eur J Pharmacol. 2015 Aug 15;761:55-64.

Redox modulation of cellular stress response and lipoxin A4 expression by Hericium Erinaceus in rat brain: relevance to Alzheimer’s disease pathogenesis.Trovato A, Siracusa R, Di Paola R, Scuto M, Ontario ML, Bua O, Di Mauro P, Toscano MA, Petralia CC, Maiolino L, Serra A, Cuzzocrea S, Calabrese V. (2016) Immun Ageing. 13:23.

ATOX1 gene silencing increases susceptibility to anticancer therapy based on copper ionophores or chelating drugs.Barresi V, Spampinato G, Musso N, Trovato Salinaro A, Rizzarelli E, Condorelli DF. J Inorg Biochem. 2016 Mar;156:145-152.

Heme oxygenase-1 nuclear translocation regulates bortezomibinduced cytotoxicity and mediates genomic instability in myeloma cells.Tibullo D, Barbagallo I, Giallongo C, Vanella L, Conticello C, Romano A, Saccone S, Godos J, Di Raimondo F, Li Volti G. Oncotarget. 2016 May 17;7(20):28868-80.

Transcriptome analysis of copper homeostasis genes reveals coordinated upregulation of SLC31A1,SCO1, and COX11 in colorectal cancer.Barresi V, Trovato-Salinaro A, Spampinato G, Musso N, Castorina S, Rizzarelli E, Condorelli DF. FEBS Open Bio. 2016 Jul 8;6(8):794-806.

Molecular Mechanisms Mediating Antiangiogenic Action of the Urokinase Receptor-Derived Peptide UPARANT in Human Retinal Endothelial Cells.Motta C, Lupo G, Rusciano D, Olivieri M, Lista L, De Rosa M, Pavone V, Anfuso CD.Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5723-5735. doi: 10.1167/iovs.16-19909.

Possible role of diet in cancer: systematic review and multiple meta-analyses of dietary patterns, lifestyle factors, and cancer risk.Grosso G, Bella F, Godos J, Sciacca S, Del Rio D, Ray S, Galvano F, Giovannucci EL. (2017) Nutr Rev 75:405-419.

Gabapentin Attenuates Ocular Inflammation: In vitro and In vivo Studies.Anfuso CD, Olivieri M, Fidilio A, Lupo G, Rusciano D, Pezzino S, Gagliano C, Drago F. and Bucolo C. Frontiers in Pharmacology 8 (2017) 173

Chromosomal instability analysis and regional tumor heterogeneity in colon cancer. Barresi V, Castorina S, Musso N, Capizzi C, Luca T, Privitera G, Condorelli DF. Cancer Genet. 2017 Jan;210:9-21.

Familial 18q12.2 deletion supports the role of RNA-binding protein CELF4 in autism spectrum disorders.Barone R, Fichera M, De Grandi M, Battaglia M, Lo Faro V, Mattina T, Rizzo R. Am J Med Genet A. 2017 - N.

Aging and Parkinson’s Disease: Inflammaging, neuroinflammation and biological remodeling as key factors in pathogenesis.Calabrese V, Santoro A, Monti D, Crupi R, Di Paola R, Latteri S, Cuzzocrea S, Zappia M, Giordano J, Calabrese EJ, Franceschi C. Free Radic Biol Med. 2018 Feb 1;115:80-91.

Worldwide trends in body-mass index, underweight, overweight, and obesity from 1975 to 2016: a pooled analysis of 2416 population-based measurement studies in 128·9 million children, adolescents, and adults. NCD Risk Factor Collaboration (NCD-RisC).Grosso G., Godos J, Galvano F. et al. The Lancet 2017 390(10113), pp. 2627-2642.

Receptor-mediated toxicity of human amylin fragment aggregated by short- and long-term incubations with copper ions.Caruso G, Distefano DA, Parlascino P, Fresta CG, Lazzarino G, Lunte SM, Nicoletti VG. Mol Cell Biochem. 2017 Jan;425(1-2):85-93.

Anti-angiogenic Therapy in Cancer: Downsides and New Pivots for Precision Medicine.Lupo G, Caporarello N, Olivieri M, Cristaldi M, Motta C, Bramanti V, Avola R, Salmeri M, Nicoletti F, Anfuso CD.Front Pharmacol. 2017 Jan 6;7:519. doi: 10.3389/fphar.2016.00519. eCollection 2016. Review.

Cytosolic and calcium-independent Phospholipases A2 activation and prostaglandins E2 are associated with Escherichia coli-induced reduction of insulin secretion in INS-1E cells.Caporarello, M. Salmeri, M. Scalia, C. Motta, C. Parrino, L. Frittitta, M. Olivieri, M. Cristaldi, R. Avola, V. Bramanti, M. A. Toscano, CD. Anfuso, and G. Lupo. PLoS ONE, 11(9) (2017) :e0159874.Jun;173(6):1649-1655.

Fusion or Fission: The Destiny of Mitochondria In Traumatic Brain Injury of Different Severities.Di Pietro V, Lazzarino G, Amorini AM, Signoretti S, Hill LJ, Porto E, Tavazzi B, Lazzarino G, Belli A. Sci Rep. 2017 Aug 23;7(1):9189.

Section of Medical Biochemistry

33Biometec: a research for life

Section of Medical BiochemistryClassical VEGF, Notch and Ang signalling in cancer angiogenesis, alternative approaches and future directions (Review).Caporarello N, Lupo G, Olivieri M, Cristaldi M, Cambria MT, Salmeri M, Anfuso CD.Mol Med Rep. 2017 Oct;16(4):4393-4402. doi: 10.3892/mmr.2017.7179. Epub 2017 Aug 7. Review. Front Pharmacol. 2017 Jan 6;7:519. doi: 10.3389/fphar.2016.00519. eCollection 2016. Review.

Cytotoxic phenanthroline derivatives alter metallostasis and redox homeostasis in neuroblastoma cells.Naletova I, Satriano C, Curci A, Margiotta N, Natile G, Arena G, La Mendola D, Nicoletti VG and Rizzarelli E. Oncotarget, 2018, Vol. 9, (No. 91), pp: 36289-36316

Comparison Between Folic Acid and gH625 Peptide-Based Functionalization of Fe3O4 Magnetic Nanoparticles for Enhanced Cell Internalization.C. Tudisco, M. T. Cambria, A. E. Giuffrida, F. Sinatra, C. D. Anfuso, G. Lupo, N. Caporarello, A. Falanga, S. Galdiero, V. Oliveri, C. Satriano, and G. G. Condorelli. Nanoscale Res Lett. 2018; 13: 45.

Water soluble glucose derivative of thiocarbohydrazone acts as ionophore with cytotoxic effects on tumor cells.Bonaccorso C, Grasso G, Musso N, Barresi V, Condorelli DF, La Mendola D, Rizzarelli E. J Inorg Biochem. 2018, 182:92-102.

Phenotypic characterization of the SIRC (Statens Seruminstitut Rabbit Cornea) cell line reveals a mixed epithelial and fibroblastic nature.Olivieri M, Cristaldi M, Pezzino S, Rusciano D, Tomasello B, Anfuso CD, Lupo G. Exp Eye Res. 2018;172:123-127.

Histone variant macroH2A1 rewires carbohydrate and lipid metabolism of hepatocellular carcinoma cells towards cancer stem cells.Lo Re O, Douet J, Buschbeck M, Fusilli C, Pazienza V, Panebianco C, Castravano CC, Mazza T, Li Volti G, Vinciguerra M. Epigenetics. 2018;13(8):829-845

Neuroinflammation and neurohormesis in the pathogenesis of Alzheimer’s disease and Alzheimer-linked pathologies: modulation by nutritional mushrooms.Trovato Salinaro A, Pennisi M, Di Paola R, Scuto M, Crupi R, Cambria MT, Ontario ML, Tomasello M, Uva M, Maiolino L, Calabrese EJ, Cuzzocrea S, Calabrese V. Immun Ageing. 2018 Feb 14;15:8.

Effect of Lipoic Acid on the Biochemical Mechanisms of Resistance to Bortezomib in SH-SY5Y Neuroblastoma Cells.Tibullo D, Giallongo C, Puglisi F, Tomassoni D, Camiolo G, Cristaldi M, Brundo MV, Anfuso CD, Lupo G, Stampone T, Li Volti G, Amenta F, Avola R, Bramanti V. Mol Neurobiol. 2018 Apr;55(4):3344-3350. doi: 10.1007/s12035-017-0575-6.

Blood-Brain Barrier in a Haemophilus influenzae Type a In Vitro Infection: Role of Adenosine Receptors A2A and A2B.Caporarello N, Olivieri M, Cristaldi M, Scalia M, Toscano MA, Genovese C, Addamo A, Salmeri M, Lupo G, Anfuso CD. Mol Neurobiol. 2018 Jun;55(6):5321-5336.

SOX2: Not always eye malformations. Severe genital but no major ocular anomalies in a female patient with the recurrent c.70del20 variant.Errichiello E, Gorgone C, Giuliano L, Iadarola B, Cosentino E, Rossato M, Kurtas NE, Delledonne M, Mattina T, Zuffardi O. Eur J Med Genet. 2018 Jun;61(6):335-340. doi: 10.1016/j.ejmg.2018.01.011. Epub 2018 Jan 31.

Age-Related Dry Eye Lactoferrin and Lactobionic Acid.Rusciano D, Pezzino S, Olivieri M, Cristaldi M, Gagliano C, Lupo G, Anfuso CD.Ophthalmic Res. 2018;60(2):94-99. doi: 10.1159/000489093. Epub 2018 Jun 19. Review. Exp Eye Res. 2018 Jul;172:123-127. doi: 10.1016/j.exer.2018.04.004. Epub 2018 Apr 11.

Transcriptome analysis reveals an altered expression profile of zinc transporters in colorectal cancer.Barresi V, Valenti G, Spampinato G, Musso N, Castorina S, Rizzarelli E, Condorelli DF. J Cell Biochem. 2018 Aug 20.

Rare variants in the genetic background modulate cognitive and developmental phenotypes in individuals carrying disease-associated variants.Pizzo L, et al. Genet Med. 2018 Sep 7

Low-molecular weight compounds in human seminal plasma as potential biomarkers of male infertility Hum Reprod.Lazzarino G, Listorti I, Muzii L, Amorini AM, Longo S, Di Stasio E, Caruso G, D’Urso S, Puglia I, Pisani G, Lazzarino G, Tavazzi B, Bilotta P. 2018 Oct 1;33(10):1817-1828.

Positive Caricature Transcriptomic Effects Associated with Broad Genomic Aberrations in Colorectal Cancer.Condorelli DF, Spampinato G, Valenti G, Musso N, Castorina S, Barresi V. Sci Rep. 2018 Oct 4;8(1):14826.

Small supernumerary marker chromosomes: A legacy of trisomy rescue?Kurtas NE, Xumerle L, Leonardelli L, Delledonne M, Brusco A, Chrzanowska K, Schinzel A, Larizza D, Guerneri S, Natacci F, Bonaglia MC, Reho P, Manolakos E, Mattina T, Soli F, Provenzano A, Al-Rikabi AH, Errichiello E, Nazaryan-Petersen L, Giglio S, Tommerup N, Liehr T, Zuffardi O. Hum Mutat. 2018 Nov 9. doi: 10.1002/humu.23683.

34 Biometec: a research for life