Section II Artificial Insemination Technique
description
Transcript of Section II Artificial Insemination Technique
Section IIArtificial Insemination
Technique
Deposit semen at the right place
90% lost due to retrograde flow (Mitchell et al. 1985)
Can horn breeding reduce retrograde loss and help to
improve fertility???
Horn vs. body depositionPositive effect No effect Negative effect
Zavos et al., 1985Lopez-Gatius and Camon-Urgel, 1988Senger et al., 1988Seidel et al., 1999 (1/5)Pursley et al., 2004Drisken et al., 2004
Elliott, 1944Brandt et al., 1946Knight et al., 1951Salisbury and VanDemark, 1951Olds et al., 1953Macpherson, 1968Williams et al., 1988Momont et al., 1989McKenna et al., 1990Lang-Ree, 1992Grieger et al., 1998Seidel et al., 1999 (4/5)Kurykin et al., 2003Andersson et al., 2004Verberckmoes et al, 2004Drisken et al., 2004
Graves et al., 1991 (P < 0.10)
Effects of horn breeding (bilateral) with low cell number doses (1 - 3 x 106) of sexed
semen on AI conception rate
0
40
80
1 2 3 4 5
Study
Perc
en
t
Body Horn
(Adapted from Seidel et al., 1999, Therio. 52:1407)
*
n = 48 50 52 48 86 86 29 32 24 14
Ipsilateral comparable to contralateral after deep unilateral semen deposition
Hawk and Tanabe, 1986
Larsson, 1986
Pallares et al.,1986
Momont et al., 1989
López-Gatius, 1996
Seidel et al., 1997
Get semen through cervix!!!
Summary
Be gentle – don’t use too much force
Two step processGet gun to the cervix
Pass cervix over the gun
Deposit semen in uterine body
Take your time
Relax
Produced by: