SECTION 2 HYDRAULICS

February 5, 2009XE-series S/M

SECTION 2 HYDRAULICS2.1 PNEUMATIC SYSTEM........................................................................................................................1

2.2 USE OF PRESSURE AND VACUUM .................................................................................................2

2.3 HYDRAULIC SYSTEM........................................................................................................................3

2.4 ANALYSIS PARAMETER ....................................................................................................................4

2.5 REAGENT SYSTEM ...........................................................................................................................6

2.6 ANALYSIS FLOW................................................................................................................................7

2.7 FCM DETECTOR BLOCK...................................................................................................................82.7.1 Function .............................................................................................................................9

2.8 OPTICAL SYSTEM ...........................................................................................................................11

2.9 HYDRAULIC SEQUENCE DESCRIPTION .......................................................................................12

2.10 HYDRAULIC PARTS .......................................................................................................................332.10.1 Solenoid Valve Function ................................................................................................332.10.2 HYDRAULIC PARTS LOCATION ..................................................................................35

2.11 SRV STRUCTURE ..........................................................................................................................40

2.12 CONVERSION TABLE OF VACUUM AND PRESSURE VALUES..................................................42

2.13 Hydraulic System Difference Between XE-2100 and XE-2100 DC ........................................442.13.1 XE-2100 Hydraulic System ............................................................................................442.13.2 XE-2100DC (XE-2100C) Hydraulic System...................................................................44

2-1 February 5, 2009XE-series S/M

SECTION 2 HYDRAULICS

2.1 PNEUMATIC SYSTEMThe system to provide the required pressure and vacuum for main unit operation consists of the following. Pressurized air is regulated at 2.5 kg/cm2 by a relief valve in the pneumatic unit, any dust and water droplets are removed by an air filter. An air drier is used to remove any remaining moisture before it is supplied to the main unit. The pressure of the dry air is regulated at 1.6, 0.7, and 0.3 kg/cm2.

The vacuum (400 mmHg or higher) is adjusted to 300 mmHg by the bellows unit in the main unit.A filter is provided on the bellows unit to prevent the needle in the bellow from accumulating dirt.

The outline of pneumatic control system is shown below.


2.2 USE OF PRESSURE AND VACUUMUse of pressure and vacuum for the main unit are as follows.

Type Name FunctionsPressure 2.5 kg/cm2 Driving master valves, air cylinders, and diaphragm pump (DP 5mL for

FCM (EPK) replenishment)

1.6 kg/cm2 Sheath Flow forming for FCM

0.7 kg/cm2 Draining waste chamber and driving of diaphragm pumps

0.3 kg/cm2 Sheath Flow forming for RBC

Vacuum Source Vacuum Aspiration of reagent and driving diaphragm pumps.

300 mmHg Aspiration of waste to WC1


2.3 HYDRAULIC SYSTEM

WBC/RBC Block Diagram

RBC/HGB Block Diagram


2.4 ANALYSIS PARAMETER

Analysis Block RBC/PLT HGB IMI

Principle Searth Flow DC Detection Method Colorimetric RF/DC

Analysis Channel

RBC/PLT Channel HGB Channel IMI Channel

Information PLT Histogram RBC Histogram

IMI Scattergram*5

Parameter PLT HCT RBC HGB -

MCV,MCH,MCHC,

Analytical Parameter

MPV,PDW, P-LCR

RDW-SD/CV

Reagent CELL PACKII / SE SEATHII SULFOLYSER STOMATOLYSER-IM

Analysis Block WBC/RET

Principle Flowcytometry by using Semi-Conductor Laser

Analysis Channel

WBC/BASO Channel

4DIFF Channel NRBC Channel

RET Channel

Information WBC/BASO Scattergram*2

4DIFF Scattergram *1 NRBC Scattergra

m*3

RET Scattergra

m*4

Parameter WBC# BASO%

BASO#

Lymph%

Lymph#

MONO%

MONO#

NEUT%

NEUT#

EOSI%

EOSI#

NRBC% NRBC#

RET% RET# PLT-

O

Analytical Parameter

L/M/HFR, IRF

Reagent STROMATOLYSER-FB

STROMATOLYSER-4DLysing solution/ Staining Dye (NEW)

STROMATOLYSER-NR Lysing

solution/ Staining

Dye (NEW)

RET SERACHII

Diluent solution/ Staining

Dye (NEW)


Figure 2-1 Scattergrams

*1 4DIFF Scattergram *2 WBC/BASO Scattergram

*3 NRBC Scattergram *4 RET Scattergram

*5 IMI Scattergram


2.5 REAGENT SYSTEM

Figure 2-2 Reaction Chamber Construction


2.6 ANALYSIS FLOW


2.7 FCM DETECTOR BLOCK

The life of the Laser Diode is approx. 10,000 hours.

NOTE:Laser Diode is very sensitive to static electricity. Follow the instruction belowwhen you replace the Laser Diode.- Remove jumper pin from the terminal on the Laser Diode AFTER connect-

ing to PCB No. 2140. If the jumper pin is removed before connecting toPCB No.2140, Laser Diode will be damaged.

- Do not disconnect the connector to the PCB No.2140.In the worst case, the device inside the Laser Diode will be destroyed.


2.7.1 Function

(1) Outline Laser diode apply to samples in sheath flow generated flow cell. FCM Detector Block detects frontscattered light, side scattered light, and side fluorescence light from these samples. XE-2100 gen-erates two-dimensional scattergrams from these signals as follows.

SSCL: Side SCattered peak LogarithmFSCL: Front SCattered peak LogarithmSFLP: Side Fluorescence Light Peak linearSFLL: Side Fluorescence Light peak LogarithmFSCP: Front SCattered Peak linear

Following table shows which receiver is used for each parameter.

(2) Beam spot generating systemBeam spot generating system consists of laser diode, collimator lens. Laser beam generated bylaser diode is converted to parallel beam by collimator lens. This beam is collected by condenserlens and applies to samples. Beam shape is as follows.

(3) Sheath flow systemSheath flow is generated in the flow cell. Sample flows center of the flow cell.

(4) Front scattered light systemFront scattered light system consists of beam stopper and FSC condenser lens. Beam stopper shade direct light from the laser diode. FSC condenser lens collect the scatteredlight of the sample.

(5) Dichroic mirrorLight below 640 nm wave length reflects to the side scattered light PMT.

X-axis Y-axis

WBC# / BASO SSCL FSCP

DIFF SSCL SFLP

NRBC SFLL FSCP

RET SFLP FSCP

PLT-O SFLP FSCL

FSC Photo diode Side Scattered PMT Side Fluorescence PMT

WBC# / BASO Used Used Not Used

DIFF Used but not displayed in Scattergram

Used Used

NRBC Used Not Used Used

RET Used Not Used Used


(6) Side fluorescence light systemSide fluorescence light system consists of interference filter, PMT (Photo Multiplier Tube). The lightthrough dichroic mirror is filtered by interference filter and fluorescence light over 660 nm wavelength is detected by PMT. Detected fluorescence light signal is amplified by pre-amplifier andtransmitted to main amplifier.

(7) Side Scattered light systemSide Scattered light system consists of ND filter (Neutral Density filter) and PMT. The light through dichroic mirror is attenuated by ND filter and scattered light is detected by PMT.Detected scattered light signal is amplified by pre-amplifier and transmitted to main amplifier.

(8) Temperature ControlFCM Detector block is heater controlled to maintain the optical stability.

(9) Cover switchCover switch is installed to prevent interference from the outer light and leakage of the laser beam.If the cover is opened, XE-2100 stops laser radiation and massage is displayed.

2-11 February 5, 2009XE-2100 S/M

2.8 OPTICAL SYSTEM

2-12 February 5, 2009XE-2100 S/M

2.9 HYDRAULIC SEQUENCE DESCRIPTION0- 5 sec[WB aspiration, reagent chamber]

- SRV turns activating SV60 for 0 - 0.5 sec.- Whole blood pump descend to aspirate diluent for 0 - 2.0 sec.- SRV is rinsed (perimeter cleaning) by activating SV60 for 0 - 0.5.sec.- WC2 is drained by activating SV 43 for 1.5 - 7.5 sec.- WC3 is drained by activating SV 45 for 0.5 - 2.0 sec.- SNR is replenished by activating SV8 for 0 - 3.0 sec.

[HGB]- Solution in HGB flow cell is drained by activating SV36 for 3.0 - 3.8 sec.- HGB Diluent DP dispense diluent into HGB flow cell for rinsing for 3.8 - 4.9 sec.- SV 11 is activated to push the solution in the air mixing tubing to WC1.

2-13 February 5, 2009XE-2100 S/M

2-14 February 5, 2009XE-2100 S/M

5 - 10 sec[WB aspiration, reagent chamber]

- Whole blood pump descend to aspirate sample in the second stage for 7.8 - 7.9sec.- SV 54 is activated to switch the path to sample line for 9.3 - 17.7 sec.- Diluent is dispensed to rinse cup by activating SV56 and drained into WC1 by activating SV59

while rinse cup descends for clean pipette outside.- WC2 is drained by activating SV 43 for 1.5 - 7.5 sec.- Sheath waste chamber is drained by activating SV 69 for 5.7 - 7.5 sec.

[BASO]- Solution in BASO reaction chamber is drained by activating SV 40 for 8.7 9.4 sec. - Lyse reagent is dispensed to BASO reaction chamber by activating SV19 for 9.4 - 10.6 sec for

rinsing.- Mixing motor at reaction chamber rotates for 9.4 - 10.5 sec.

[DIFF]- Solution in DIFF reaction chamber is drained by activating SV38 for 8.7 - 9.4 sec.- Lyse reagent is dispensed to DIFF reaction chamber by activating SV34 for 9.4 - 10.6 sec for rins-

ing.

[NRBC]- Solution in NRBC reaction chamber is drained by activating SV39 for 8.7 - 9.4 sec.- Diluent is dispensed to NRBC reaction chamber by activating SV34 for 9.4 - 10.6 sec for rinsing.

[RET]- Solution in RET reaction chamber is drained by activating SV37 for 8.5 - 9.1 sec.- Diluent is dispensed into RET reaction chamber by activating SV32 for 9.6 - 10.6 sec for rinsing.- Diluent is dispensed into RET reaction chamber by activating SV49 for 9.1 - 9.6 sec for rinsing.

[HGB]- Solution in HGB flow cell is drained by activating SV36 for 4.9 - 5.8 sec.- HGB Diluent DP dispense diluent into HGB flow cell for rinsing for 5.8 - 7.8 sec.

[RBC]- Solution in RBC sample chamber is drained by activating SV65 for 6.7 - 8.2 sec.- Solution in RBC sample chamber is drained by activating SV65 for 9.7 - 11.4 sec.- RBC Diluent DP dispense diluent into RBC sample chamber for 8.2 - 9.7 sec.

[IMI]- Solution in IMI detector chamber is drained by activating SV14 for 7.5 - 9.1 sec.- IMI lyse reagent DP dispense reagent into IMI detector chamber for 7.5 - 8.4 sec.- IMI lyse reagent DP dispense reagent into IMI detector chamber for 8.4 - 10.2 sec.- IMI detector chamber is air mixed by activating SV12.

2-15 February 5, 2009XE-2100 S/M

2-16 February 5, 2009XE-2100 S/M

10 - 15 sec.[WB aspiration, reagent chamber]

- Whole blood pump descend to aspirate diluent for 9.3 - 12.3 sec.- SV 54 is activated to switch the path to sample line for 9.3 - 17.7 sec.- Solution in WC1 is drained by activating SV57 for 11.8 - 14.6 sec.- EPK is replenished by activating SV 10 for 12.6 - 16.4 sec.

[BASO]- BASO reaction chamber is air mixed by activating SV13.- Solution in BASO reaction chamber is drained by activating SV40 for 10.5 - 14.6 sec.- Lyse reagent is dispensed with sample to BASO reaction chamber by activating SV19 for 11.6 -

14.1 sec.- Mixing motor at reaction chamber rotates for 11.3 - 14.9 sec for sample mixing.

[DIFF]- Solution in DIFF reaction chamber is drained by activating SV38 for 10.5 - 11.5 sec.- Lyse reagent is dispensed with sample to DIFF reaction chamber by activating SV33 for 11.6 -

14.1 sec.- Stain reagent is dispensed with sample to DIFF reaction chamber by activating SV63 for 12.5 -

14.1.[NRBC]

- Solution in NRBC reaction chamber is drained by activating SV39 for 10.5 - 11.5 sec.- Diluent is dispensed with sample to NRBC reaction chamber by activating SV34 for 11.6 - 14.1

sec.- Stain reagent is dispensed with sample to NRBC reaction chamber by activating SV62 for 12.5 -

14.1.

[RET]- Solution in RET reaction chamber is drained by activating SV37 for 10.6 - 11.7 sec.- Diluent is dispensed with sample into RET reaction chamber by activating SV32 for 11.8 - 14.3

sec for rinsing.- Stain reagent is dispensed with sample to RET reaction chamber by activating SV64 for 12.7 -

14.3.

[HGB]- Solution in HGB flow cell is drained with sample by activating SV36 for 10.0 - 11.4 sec.- HGB Diluent DP dispense diluent into HGB flow cell by activating SV15 for 11.4 - 14.0 sec.- HGB lyse DP dispense diluent into HGB flow cell by activating for 11.4 - 14.0 sec.- HGB flow cell is air mixed by activating SV11.

[RBC]- Solution in RBC sample chamber is drained by activating SV65 for 9.7 - 11.4 sec.- RBC Diluent DP dispense diluent into RBC sample chamber for 11.4 - 14.3 sec.- RBC sample is charged by activating SV67 and SV68 for 14.4 - 15.9 sec.- RBC sheath form is generated by activating SV70 for13.5 - 36.4 sec.- Front flash activated by SV71 for 13.3 - 14.3 sec.- Back flash activated by SV72 for 13.4 - 13.7 sec.

2-17 February 5, 2009XE-2100 S/M

[IMI]- Solution in IMI detector chamber is drained by activating SV14 for 10.2 - 11.4 sec.- IMI lyse reagent DP dispense reagent into IMI detector chamber for 8.4 - 10.2 sec.- IMI lyse reagent DP dispense reagent into IMI detector chamber for 11.4 - 15.0 sec.- IMI detector chamber is air mixed by activating SV12.

2-18 February 5, 2009XE-2100 S/M

2-19 February 5, 2009XE-2100 S/M

15 - 20 Sec[WB aspiration, reagent chamber]

- Whole blood pump ascend to rinse sample line for 17.7 - 19.7 sec. (moves slowly to prevent drop-ping sample from the pipette tip.)

- SV 54 is activated to switch the path to sample line for 9.3 - 17.7 sec.- SRV is rinsed (perimeter cleaning) by activating SV60 for 15.7 - 16.7.sec.- Diluent is dispensed to rinse cup by activating SV56 and drained into WC1 by activating SV59.- WC3 is drained by activating SV 45 for 15.5 - 17.5 sec.- EPK is replenished by activating SV 10 for 12.6 - 16.4 sec.- SLS, SIM, FBT,FFD, and RED reagent is replenished by activating SV1, 4, 5, 6, 7, and 9.- SNR is replenished by activating SV8 for 17.5 - 18.5 sec.- Diluent is dispensed to NRBC reaction chamber by activating SV46 for 17.2 - 17.4 sec.

[NRBC]- NRBC sample is charged to sheath sample line by activating SV47 and SV 24 for 16.4 - 17.5 sec.- Sheath syringe ascends for 17.5 - 22.5 sec.- Low speed sheath form is generated by SV28 and SV20 from 15 sec and after.- High speed sheath form is generated by SV29 and SV20 for 17.7 - 22.6 sec.- NRBC is counted for 18.8 - 22.5 sec.- Sheath syringe is rinsed by SV53 for 17.3 - sec.- EPK is replenished by activating SV3 for 17.0 - 18.2 sec.- Sheath syringe is ascend for 17.5 - 22.5 sec.- NRBC is counted for 18.8 - 22.5 sec.

[RBC]- Solution in RBC sample chamber is drained by activating SV65 for 9.7 - 11.4 sec.- RBC Diluent DP dispense diluent into RBC sample chamber for 11.4 - 14.3 sec.- RBC sample is charged by activating SV67 and SV68 for 14.4 - 15.9 sec.- RBC sheath form is generated by activating SV70 for13.5 - 36.4 sec.- Sheath syringe ascends for 15.9 - 35.8 sec.

2-20 February 5, 2009XE-2100 S/M

2-21 February 5, 2009XE-2100 S/M

20 - 25 sec.[WB aspiration, reagent chamber]

- Whole blood pump ascend to rinse sample line for 19.7 - 21.6 sec.- Whole blood pump descend to make air gap for 22.6 - 23.1 sec.- Diluent is dispensed to rinse cup by activating SV56 and drained into WC1 by activating SV59

while rinse cup ascends for clean pipette outside.- EPK is replenished by activating SV 10 for 21.2 - 23.5 sec.- Water in the air filter is drained by activating SV55 for 23.9 - 24.0 sec.

[BASO]- Diluent is dispensed to BASO reaction chamber by activating SV52 for 24.3 - 24.5 sec.- Charge line is rinsed by activating SV21 and SV22.- Sample is charged by activating SV16 and SV24.- Sheath syringe descends for 22.6 - 24.6 for preparing BASO counting.- Sheath syringe line is rinsed by activating SV51 for 22.5 - 23.2 sec.- EPK is replenished by activating SV3 for 22.5 - 23.7 and 24.0 - 25.1 sec.

[RBC]- RBC sheath form is generated by activating SV70 for13.5 - 36.4 sec.- Sheath syringe ascends for 15.9 - 35.8 sec.- RBC counts for 23.0 - 35.8 sec.

[IMI]- IMI manometer is filled by activating SV 17 for 22.9 - 31.3 sec.

2-22 February 5, 2009XE-2100 S/M

2-23 February 5, 2009XE-2100 S/M

25 - 30 sec[BASO]

- Sheath syringe ascends for 24.6 - 30.6 sec.- Low speed sheath form is generated by SV28 and SV20 from 15 sec and after.- High speed sheath form is generated by SV29 and SV20 for 17.7 - 22.6 sec.- BASO is counted for 26.1 - 30.5 sec.

[HGB]- HGB converts for 26.0 - 27.0 sec.


[IMI]- IMI manometer is filled by activating SV 17 for 22.9 - 31.3 sec.

2-24 February 5, 2009XE-2100 S/M

2-25 February 5, 2009XE-2100 S/M

30 - 35 sec[DIFF]

- Diluent is dispensed to DIFF chamber by activating SV50 for 32.1 32.3 sec.- Charge line is rinsed by activating SV21 and 24 for 30.5 - 31.2 sec.- DIFF Sample is charged by activating SV31 and 24 for 31.4 - 32.4 sec.- Sheath syringe ascends for 32.4 - 38.3 sec.- Low speed sheath form is generated by SV28 and SV20 from 15 sec and after.- High speed sheath form is generated by SV29 and SV20 for 32.6 - 38.4 sec.- DIFF is counted for 18.8 - 22.5 sec.- Sheath syringe (low speed) is rinsed by SV53 for 32.2 - 32.7 sec.- Sheath syringe (high speed) is rinsed by SV51 for 30.5 - 31.2 sec.- EPK is replenished by activating SV3 for 30.5 - 32.1 sec 32.1 - 33.0 sec.


2-26 February 5, 2009XE-2100 S/M

2-27 February 5, 2009XE-2100 S/M

35 - 40 sec[RET]

- Diluent is dispensed to RET reaction chamber by activating SV49 for 39.9 - 40.0 sec.- RET sample is charged by activating SV35 and 24 for 39.2 - 40.3 sec.- Charge line is rinsed by activating SV21 and 24 for 30.5 - 31.2 sec.- Sheath syringe ascends for 39.9 - 46.2 sec.- Low speed sheath form is generated by SV28 and SV20 from 15 sec and after.- RET is counted for 43.0 - 46.2 sec.- Sheath syringe (low speed) is rinsed by SV53 for 40.1 - 40.4 sec.- Sheath syringe (high speed) is rinsed by SV53 for 38.3 - 39.0 sec.- EPK is replenished by activating SV3 for 38.3 - 39.5 and 39.7 - 40.9 sec.

[RBC]- RBC sheath form is generated by activating SV70 for13.5 - 36.4 sec.- Sheath syringe ascends for 15.9 - 35.8 sec.- RBC counts for 23.0 - 35.8 sec.- RBC sheath syringe is rinsed by activating SV 68 and 66 for 35.9 - 36.4 sec.- ESE is replenished by activating SV2 for 35.9 - 37.9 sec.- RBC sheath syringe descends for 35.9 - 39.0 sec.

2-28 February 5, 2009XE-2100 S/M

2-29 February 5, 2009XE-2100 S/M

40 - 45 sec[RET]

- Diluent is dispensed to RET reaction chamber by activating SV49 for 39.9 - 40.0 sec.- RET sample is charged by activating SV35 and 24 for 39.2 - 40.3 sec.- Charge line is rinsed by activating SV21 and 24 for 30.5 - 31.2 sec.- Sheath syringe ascends for 39.9 - 46.2 sec.- Low speed sheath form is generated by SV28 and SV20 from 15 sec and after.- RET is counted for 43.0 - 46.2 sec.- Sheath syringe (low speed) is rinsed by SV53 for 40.1 - 40.4 sec.- Sheath syringe (high speed) is rinsed by SV53 for 38.3 - 39.0 sec.- EPK is replenished by activating SV3 for 38.3 - 39.5 and 39.8 - 41.0 sec.

2-30 February 5, 2009XE-2100 S/M

2-31 February 5, 2009XE-2100 S/M

45 - 50 sec[RET]

- Charge line is rinsed by activating SV21, 22, and 51 for 47.1 - 48.1 sec.- Sheath syringe ascends for 39.9 - 46.2 sec.- Low speed sheath form is generated by SV28 and SV20 from 15 sec and after.- RET is counted for 43.0 - 46.2 sec.- RET background is counted for 46.4 - 47.1 sec to subtract the background value from the sample

value to eliminate the high background effect.- EPK is replenished by activating SV3 for 38.3 - 39.5 and 39.8 - 41.0 sec.

2-32 February 5, 2009XE-2100 S/M

2-33 February 5, 2009XE-2100 S/M

2.10 HYDRAULIC PARTS

2.10.1 Solenoid Valve Function

SVNo

Function LocatedValveUnit

Page SVNo.


Page

1 SLS replenishment 139 2-36 42 ESE outer float bubble removal

136 2-34

2 ESE replenishment 139 2-36 43 WC2 (FCM sheath waste) drain

136 2-34

3 EPK (FCM Sheath) replenishment

139 2-36 44 SIM outer float bubble removal

136 2-34

4 FBT replenishment 139 2-36 45 WC3 (IMI waste) drain 136 2-34

5 SIM replenishment 139 2-36 46 NRBC reaction chamber rinse

136 2-34

6 apply vacuum to Reagent chambers

139 2-36 47 NRBC charging line open

136 2-34

7 4DL replenishment 139 2-36 48 Latex for OPT align/CELL CLEAN for SHUTDOWN

136 2-34

8 SNR replenishment 139 2-36 49 RET reaction chamber rinse (discrete)

136 2-34

9 RED replenishment 139 2-36 50 DIFF reaction chamber rinse(discrete)

136 2-34

10 EPK (Diluent) replenishment

139 2-36 51 FCM sheath syringe intense rinse

136 2-34

11 HGB air mixing 134 2-33 52 BASO reaction chamber rinse(discrete)

136 2-34

12 IMI air mixing 134 2-33 53 FCM sheath syringe rinse

136 2-34

13 BASO air mixing 134 2-33 54 WB aspirates (OFF for SRV)

136 2-34

14 IMI draining 134 2-33 55 FCM Sheath, RBC bubble removal 2.5Kg/cm2 drain)

137 2-34

15 HGB dilution DP dispense

134 2-33 56 Dispense rinse solution to manual rinse cup

137 2-34

16 BASO charging line open

134 2-33 57 WC1 drain 137 2-34

17 IMI count 134 2-33 58 CP rinse cup drain 137 2-34

18 RBC dilution DP dispense

134 2-33 59 Manual rinse cup drain 137 2-34

19 BASO dilution DP dispense

134 2-33 60 SRV perimeter rinse 137 2-34

20 FCM sheath output port open

134 2-33 61 IMI (discrete) rinse 137 2-34

21 FCM Rinse DP drive (Rinse)

134 2-33 62 NRBC dye DP dispense

138 2-35

22 FCM charging line switch

134 2-33 63 DIFF dye DP dispense 138 2-35

2-34 February 5, 2009XE-2100 S/M

23 SRV turn (ascend on ON)

134 2-33 64 RET dye DP dispense 138 2-35

24 FCM charging DP drive 134 2-33 65 RBC sample chamber drain

133 2-33

25 IMI clog removal air ON 134 2-33 66 RBC sheath syringe rinse

133 2-33

26 IMI filling 134 2-33 67 RBC sample charging 133 2-33

27 SLS DP drive 135 2-34 68 RBC charging DP activate

133 2-33

28 Generate low speed sheath

135 2-34 69 RBC sheath WC drain 133 2-33

29 Generate high speed sheath

135 2-34 70 RBC sheath generate 133 2-33

30 IMI DP drive 135 2-34 71 RBC front flash & transducer bubble removal

133 2-33

31 DIFF charging line open

135 2-34 72 RBC back flash 133 2-33

32 RED DP dispense 135 2-34 73 Waste container sensor drain

--- ---

33 DIFF diluent DP dispense

135 2-34 74 Not used --- ---

34 NRBC diluent DP dispense

135 2-34 CP1 Apply pressure air to CP needle

CP Unit ---

35 RET charging line open 135 2-34 CP2 Dispense rinse solution to CP needle

CP Unit ---

36 HGB drain 135 2-34 CP3 CP needle pierce CP Unit ---

37 RET reaction chamber drain

135 2-34 CP4 CP clump CP Unit ---

38 DIFF reaction chamber drain

135 2-34 CP5 CP hand turning mixing CP Unit ---

39 NRBC reaction chamber drain

135 2-34 CP6 CP hand forward / backward (ON for forward)

CP Unit ---

40 BASO reaction chamber drain

135 2-34 CP7 CP hand descend CP Unit ---

41 FCM flow cell air bubble remove

135 2-34 CP8 CP hand ascend CP Unit ---

SVNo


Page SVNo.


Page

2-35 February 5, 2009XE-2100 S/M

2.10.2 HYDRAULIC PARTS LOCATION

Left side

2-36 February 5, 2009XE-2100 S/M

Valve Unit No.133

2-37 February 5, 2009XE-2100 S/M

Valve Unit No. 134

Valve Unit No.135 (ECR 308F056 not applied)

Valve Unit No.135 (ECR 308F056 applied)

2-38 February 5, 2009XE-2100 S/M

Valve Unit No.136 (ECR 308F056 not applied)

Valve Unit No.136 (ECR 308F056 applied)

Valve Unit No.137

2-39 February 5, 2009XE-2100 S/M

Valve Unit No.138

Valve Unit No. 139

2-40 February 5, 2009XE-2100 S/M

2.11 SRV STRUCTURE

2-41 February 5, 2009XE-2100 S/M

2-42 February 5, 2009XE-2100 S/M

2.12 CONVERSION TABLE OF VACUUM AND PRESSURE VALUESConversion formulas for vacuum and pressure values are shown as following (1) and (2). When the value is not listed, calculate and obtain those values. (1) Vacuum (y) in SI unit (MPa) = Vacuum (x) in conventional unit (mmHg) * 0.000133322

(2) Pressure (Y) in SI unit (MPa) = Pressure (X) in conventional unit (kg/cm2) * 0.0980665

Vacuum in Conventional Unit (x)

Vacuum in SI Unit (y)

Pressure in Conventional Unit (X)

Pressure in SI Unit (Y)

5 mmHg 0.0007 MPa 0.001 kg/cm2 0.0001 MPa

10 mmHg 0.0013 MPa 0.003 kg/cm2 0.0003 MPa

20 mmHg 0.0027 MPa 0.005 kg/cm2 0.0005 MPa

35 mmHg 0.0047 MPa 0.01 kg/cm2 0.0010 MPa

100 mmHg 0.0133 MPa 0.03 kg/cm2 0.0029 MPa

150 mmHg 0.0200 MPa 0.05 kg/cm2 0.0049 MPa

180 mmHg 0.0240 MPa 0.07 kg/cm2 0.0069 MPa

200 mmHg 0.0267 MPa 0.1 kg/cm2 0.0098 MPa

215 mmHg 0.0287 MPa 0.2 kg/cm2 0.0196 MPa

220 mmHg 0.0293 MPa 0.25 kg/cm2 0.0245 MPa

230 mmHg 0.0307 MPa 0.27 kg/cm2 0.0265 MPa

240 mmHg 0.0320 MPa 0.300 kg/cm2 0.0294 MPa

248 mmHg 0.0331 MPa 0.301 kg/cm2 0.0295 MPa

250 mmHg 0.0333 MPa 0.33 kg/cm2 0.0324 MPa

252 mmHg 0.0336 MPa 0.35 kg/cm2 0.0343 MPa

260 mmHg 0.0347 MPa 0.380 kg/cm2 0.0373 MPa

270 mmHg 0.0360 MPa 0.400 kg/cm2 0.0392 MPa

280 mmHg 0.0373 MPa 0.43 kg/cm2 0.0422 MPa

290 mmHg 0.0387 MPa 0.45 kg/cm2 0.0441 MPa

298 mmHg 0.0397 MPa 0.47 kg/cm2 0.0461 MPa

299 mmHg 0.0399 MPa 0.497 kg/cm2 0.0487 MPa

300 mmHg 0.0400 MPa 0.500 kg/cm2 0.0490 MPa

380 mmHg 0.0507 MPa 0.510 kg/cm2 0.0500 MPa

390 mmHg 0.0520 MPa 0.52 kg/cm2 0.0510 MPa

400 mmHg 0.0533 MPa 0.53 kg/cm2 0.0520 MPa

415 mmHg 0.0553 MPa 0.55 kg/cm2 0.0539 MPa

420 mmHg 0.0560 MPa 0.57 kg/cm2 0.0559 MPa

440 mmHg 0.0587 MPa 0.60 kg/cm2 0.0588 MPa

450 mmHg 0.0600 MPa 0.605 kg/cm2 0.0593 MPa

500 mmHg 0.0667 MPa 0.62 kg/cm2 0.0608 MPa

510 mmHg 0.0680 MPa 0.622 kg/cm2 0.0610 MPa

530 mmHg 0.0707 MPa 0.650 kg/cm2 0.0637 MPa

550 mmHg 0.0733 MPa 0.665 kg/cm2 0.0652 MPa

600 mmHg 0.0800 MPa 0.70 kg/cm2 0.0686 MPa

2-43 February 5, 2009XE-2100 S/M

0.701 kg/cm2 0.0687 MPa

0.750 kg/cm2 0.0735 MPa

1.000 kg/cm2 0.0981 MPa

1.030 kg/cm2 0.1010 MPa

1.05 kg/cm2 0.1030 MPa

1.1 kg/cm2 0.1079 MPa

1.5 kg/cm2 0.1471 MPa

1.588 kg/cm2 0.1557 MPa

1.60 kg/cm2 0.1569 MPa

1.601 kg/cm2 0.1570 MPa

1.7 kg/cm2 0.1667 MPa

1.80 kg/cm2 0.1765 MPa

1.9 kg/cm2 0.1863 MPa

2.000 kg/cm2 0.1961 MPa

2.010 kg/cm2 0.1971 MPa

2.1 kg/cm2 0.2059 MPa

2.15 kg/cm2 0.2108 MPa

2.200 kg/cm2 0.2157 MPa

2.240 kg/cm2 0.2197 MPa

2.25 kg/cm2 0.2206 MPa

2.30 kg/cm2 0.2256 MPa

2.4 kg/cm2 0.2354 MPa

2.492 kg/cm2 0.2444 MPa

2.5 kg/cm2 0.2452 MPa

2.513 kg/cm2 0.2464 MPa

2.533 kg/cm2 0.2484 MPa

2.7 kg/cm2 0.2648 MPa

2.8 kg/cm2 0.2746 MPa

3.0 kg/cm2 0.2942 MPa

Vacuum in Conventional Unit (x)

Vacuum in SI Unit (y)

Pressure in Conventional Unit (X)

Pressure in SI Unit (Y)


2.13 Hydraulic System Difference Between XE-2100 and

XE-2100 DC

2.13.1 XE-2100 Hydraulic System

2.13.2 XE-2100DC (XE-2100C) Hydraulic System

SECTION 2 HYDRAULICS

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