SEADM DMA

SEADM supercritical DMA works at atmospheric pressure so direct API coupling is possible with minor MS interface modifications.

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Convert an existing mass spectrometer into an hybrid Ion Mobility-Mass Spectrometry instrument

Sociedad Europea de Análisis Diferencial de Movilidad S.L. www.seadm.com

arturo.alvaro@seadm.com

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Introduction – SEADM’s proposal

Are you involved in structural elucidation of molecules, particle

or nanoparticle analysis, atmospheric research, or polymer

technology?

Do you need an improvement on your MS (mass spectrometer

) selectivity responding much faster than gas or liquid

chromatography?

Are you thinking of adding mobility dimension to your

analysis?

SEADM’s supercritical DMA (Differential mobility analyzer) may

be the upgrade you are looking for.

In our eagerness to be able to analyze both atmospheric and

biological samples at the challenging fentogram level, we have

faced sensitivity and selectivity problems unsolved by

commercial devices. In collaboration with colleagues at Yale

University, we have developed high resolution supercritical

mobility analyzers that allow for unprecedented selectivity and

sensitivity for rapid measurements.

The DMA measures true linear mobility and therefore is a

powerful tool for structural studies of biomolecules.

Adding the mobility dimension enables mass distribution

measurements in industrial polymers where pure mass spectra

are generally unresolvable at moderate and large molecular

weights.

In the field of explosive trace detection in air, an important area

of expertise for SEADM, mobility analysis together with high

sensitivity MS leads to detection limits below 1pg/m3 in some

cases.

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Comercial options available

Enhanced ionization option: Instead of the basic ionization chamber, for high sensitivity volatile analysis, an improved LFSESI (Low flow secondary electrospray ionization) or a full metal chamber for APCI can be purchased. Both include temperature control and can be used as nano ESI, SESI, or APCI. The ionization efficiency can reach values of 1/700 (in the case of TNT vapors in a LFSESI).

DMA basic system: The basic system consist of a fully operative DMA device ready to be plugged to a MS in the list below with minor modification of MS interface. With a modest level of effort the DMA may be coupled essentially to any MS with an atmospheric pressure interface. The basic system includes all HV supplies, pressure and flow controls, clean recirculation pump and circuit, and a basic ionization chamber for either nano-spray or corona discharge APCI. Only standard power supply and around 1 lpm extra clean gas is required. DMA-MS integration: Choose your MS We have experience coupling DMAs to the following MS: - Shimadzu LCMS 2010 de. -ABSciex TOF Qstar -ABSciex Qtrap 3200, 4500 & 5500 -ABSciex Triple Quad 5000 & 5500 -If your favorite MS is not in this list, we will carry out the integration study for you.

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High pressure desolvation option: For samples producing substantial solid residues, or when solvent droplets are a problem, there is a version of the basic DMA with an additional mobility stage with the double function of drying and filtering sample droplets. It is only available with LFSESI chamber and requires up to 30 lpm extra clean gas supply.

Standalone devices for developers: If you are a developer and you are interested in a custom or standalone device, there is a range of products we can offer you, including air sampling devices, ionization chambers, volatility analyzers, cylindrical DMAs... please feel free to contact us and we will be happy to study your application and suggest you the best option.

Supercritical Planar Mobility Analyzer - Technology Advanced design The geometry and aerodynamics of the DMA have been carefully optimized to deliver a laminar flow at Reynolds number up to 200,000, achieving resolving powers >50 The potential of DMAs for molecular cross section studies, mass pre-filtering and noise reduction have been traditionally limited by low resolution at nanometric sizes, a difficulty now resolved by use of supercritical DMAs. These devices keep the flow laminar at Reynolds numbers considerably higher than 2,000.

Color scale: Velocity magnitude in m/s

Careful manufacturing All manufacturing improvements discovered over the years are implemented on SEADM’s DMAs. Robust architecture, mirror polishing, micro slots or whole thermal control are only some of the improvements included on SEADM’s DMAs. These refinements are the step forward towards accurate and reliable mobility measurement at atmospheric pressure.

High performance about 50% transmission, 1,000 dynamic range and resolving power up to 100 at 1nm. The standalone DMA is capable of distinguishing quantities of around 1ppb of many molecules such as TNT. Combined with MS the limit of detection of TNT reaches 1ppq. The improved software for post signal processing permits isomeric distinction between ions only separated by 1x10-6m2/V/s (mobility units).

(a) Robust design tested under extreme conditions (bumpy journeys, high temperature…)

(b) Mirror polishing to delay turbulent transition. (c) Micro slots to maximize resolution.

(d)

(e)

(b)

(c)

(a)

(d) Spectra of various tetra-alkyl-ammonium positive ions clearly separated by mobility.

(e) Isomeric species of TNT present at tiny quantities on atmospheric air only distinguished from real TNT trough mobility analysis. 5

Ionization Chamber: SEADM is specialized on nanoESI & LFSESI, but other techniques such as

APCI are also possible

DMA- MS Interface: Easy and fast connection without breaking vacuum. You can return to basic

MS configuration in a few minutes.

Planar DMA: Inlet and outlet slots are accessible allowing high transmission and easy integration with the ionization chamber and the mass spectrometer.

Mass Spectrometer: The coupling with commercial MS lets you take advantage of

state of the art technology changing every day.

Tandem system – 2D analysis Synergy between DMA – MS for breakthrough figures A combined DMA-MS instrument in triple quadrupole mode is capable of reaching unimaginable sensitivity levels on this field until now. It allows molecule, cluster and nanoparticle identification up to several nm diameter with sensitivities below 1pg/m3.

Sample 2D fingerprint A complex sample can be simultaneously mass and mobility analyzed. The DMA enables 2D analysis of any sample with any MS. Furthermore, fast 2D discovery mode is possible with TOF and Trap spectrometers. In this mode 2D plot can be obtained in minutes. Two-dimensional plot of ion

abundance (color scale) as a function of mass/charge (y axis) and DMA voltage (x axis). Each light region corresponds to an ion.

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Our commitment: Your scientific success

Sociedad Europea de Analisis Diferencial de Movilidad SL

(SEADM) is a Spanish SME founded in 2005 with the aim of

developing a new generation of analytical instruments able to

detect trace ambient vapors at the subfemtogram level, both in the

atmosphere or within the body fluids, based on extensions of prior

developments at Yale University.

We have an eight-year trajectory developing state of the art

mobility analyzers, and ionization chambers. There is also an

important area of expertise in SEADM on explosive trace detection

in ambient air.

For more details on scientific uses of our technologies see

http://www.seadm.com/papers.html

The range of products we offer goes from the basic and affordable

standalone DMA, to the complete ultra-sensitive detection system

with all the modules and the cutting-edge spectrometers, also

including the handy option of the DMA as an add-on to your

current spectrometer.

It is of SEADM highest interest to cooperate with your research

projects, so please feel free to contact us, and we’ll try to offer you

the best option for your required application.

For more information visit: www.seadm.com

For Research Use Only. Must be handled by trained personnel. Contact: Arturo Alvaro Carballido, R&D Manager (SEADM) Phone : +34 983130707 e-mail: arturo.alvaro@seadm.com Sociedad Europea de Analisis Diferencial de Movilidad S.L. | www.SEADM.com Parque Tecnologico de Boecillo. Parcela 205. 47151 Valladolid, Spain.

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8 Sociedad Europea de Analisis Diferencial de Movilidad S.L. | www.SEADM.com Parque Tecnologico de Boecillo. Parcela 205. 47151 Valladolid, Spain. Phone : +34 983130707 e-mail: arturo.alvaro@seadm.com