SCHEME OF TEACHING AND EXAMINATION B.E. V SEMESTER … 2018-19.pdf · U.Satyanarayana and U....

SCHEME OF TEACHING AND EXAMINATION

B.E. V S EMESTER (2018-19)

Elective -1

UBT521E: Environmental BT UBT523E: Operating system and DBMS

UBT522E: Biomedical Instrumentation UBT525E: Stem cell technology

Sl.

No.

Subject

Code

Subject Credits Hours/Week Examination

Marks

Lecture Tutoria

l

Practica

l

CI

E

SEE Tota

l 1 UBT518C Enzymology(PC) 3 3 0 0 50 50 100

2 UBT503C Bioinformatics(PC) 4 3 2 0 50 50 100

3 UBT504C Genetic Engineering &

Applications(PC)

4 4 0 0 50 50 100

4 UBT516C Bioprocess & Reaction

Engg. (PC)

4 3 2 0 50 50 100

5 UBT521E Elective –1 3 3 0 0 50 50 100

6 UBT506H Industrial safety and bioethics

3 3 0 0 50 50 100

7 UBT508L Bioinformatics Lab 1.5 0 0 3 50 50 100

8 UBT509L Genetic Engineering

Lab

1.5 0 0 3 50 50 100

Total

24 19 4 06 400 400 800

UBT518C: ENZYMOLOGY

3 Credits (3-0-0)

Pre requisites : Biochemistry, Structural biology

UNIT- 1

INTRODUCTION TO ENZYMES: L-10 Hours

Definition, Classification of Enzymes; Chemical nature and properties of enzymes, Factors affecting Enzyme activities: effect of temperature, pH, concentration. of substrate, concentration

of enzyme, Active site and allosteric site: Functions and properties , coenzymes and cofactors: Functions and properties, Isoenzymes, Unit of Enzyme activity.

UNIT- 2

ENZYMATIC TECHNIQUES: L-10 Hours Strategies of purification of enzymes: choice of source, methods of homogenization, methods of

separation, Criteria of purity: tests for purity, tests for catalytic activity, active site titrations, Molecular weight determination and characterization of enzymes. Enzyme assay: continuous and discontinuous assay with examples.

UNIT- 3 ENZYME SPECIFICITY: L-10 Hours Types of Enzyme specificities. Enzyme substrate reactions. Mechanism of enzyme action- lock

and key model, Induced fit hypothesis, substrate strain theory (with lysozyme as a typical example). Enzyme kinetics Derivations of Km value (Michaelis-Menton constant), Lineweaver-

Burk plot.Mechanism of Enzyme catalysis - Acid-Base catalysis, covalent catalysis and -entropy effect. and Enzyme inhibition and kinetics.

UNIT- 4

MULTI-S UBSTRATE REACTIONS: L-10 Hours Regulation of enzyme activity,Allosteric regulation, feedback regulation, cascade system

(genetic regulation), Introduction to enzyme catalyzed reaction Ping-pong mechanism, Sequential mechanism (ordered and random), Enzyme models - Host guest complexation chemistry.

Total: 40Hours

TEXT BOOKS:

1. T Palmer,P L Bonner “Enzymes: Biochemistry , Biotechnology, Clinical Chemistry” 2nd Edition, Woodhead Publishing, 2007

2. Nicholas C. Price and Lewis Stevens “Fundamentals of enzymology, 3rd Edition,Oxford University Press,1999

REFERENCE BOOKS: 1. Daniel L. Purich Contemporary Enzyme Kinetics and Mechanism 3rd Edition, Academic

Press, 2009

2. K. Faber Biotransformations 7th Edition Springer- Verlag publication,2018 3. U.Satyanarayana and U. Chakrapani “Biochemistry “5th edition, Elsevier Books and Allied,

2017

4. David L. Nelson and Michael Cox, “Lehninger Principles of Biochemistry” –7th Edition, W H Freeman & Co.,2017

COURS E OUTCOMES : At the end of the course students should able to:

1. Define Enzymes and explain their properties and functions. 2. Classify and characterize the enzymes. 3. Choose Isolation and purification techniques for enzymes.

4. Design and conduct experiments, interpret, analyse data and report results. 5. Derive and Calculate Vmax and Km values of enzyme catalysed reactions.

6. Explain mechanisms of enzyme action and its regulatory mechanism.

QUESTION PAPER PATTERN OF CIE (Continuous Internal Evaluation):

1. CIE comprises of 3 tests, each of 30 marks and I hr duration, totaling to 90 marks and later is scaled down to 45 marks

2. Each CIE Will be Covering Complete unit

3. Any two full questions to be answered out of three questions and each question carries fifteen marks

4. Assignment/ Quiz/objective tests carries five marks

QUESTION PAPER RATTERN OF S EE

Total of Eight Question with Two from each unit to be set uniformly covering the entire

syllabus.

Each question should not have more than four sub questions.

Each full question carries 20 marks Any five full questions are to be answered choosing at least one from each unit.

Course Outcomes

Programme Outcomes

Programme Specific Outcomes

1 2 3 4 5 6 7 8 9 10 11 12 PSO1 PSO2 PSO3

CO 1 1 3 2 1 2 CO 2 2 3 1 1 1 1 3 CO 3 2 3 2 2 2 3 1

CO 4 2 3 3 1 3 2 3 1 3 3 2

CO 5 3 1 3 1 3

CO 6 1 3 3 2 2 1 3

UBT503C: BIOINFORMATICS

4 Credits (4-0-0)

Prerequisites : Biochemistry Molecular biology,

Bio-statistics, Cell biology & Genetics, Computer Applications UNIT- 1

BIOLOGICAL DATABAS ES L-13Hours

Introduction to bioinformatics, meaning of databases, types of databases. The nucleotide and protein sequence Databases: GenBank, DDBJ and EMBL. Primary and Secondary sequences

databases: (SWISS PROT, PIR, NRL3D, PROSITE, PRINTS, BLOCKS and Pfam), ExPASy, Structure databases: Protein Data Bank (PDB), CATH, SCOP. File format, contents, search of databases- Gene bank flat file, PDB flat file, PIR format, FASTA Format. Structure file formats:

PDBSUM, PDBLite and MMDB Specialized databases: NCBI-PubMed, PubChem, OMIM, OMIA, Metabolic Pathway-KEGG, EST databases, HPD and SGD databases.

Tutorials: Practices on primary, secondary databases and Gen bank files and PDB file formats formats

UNIT- 2

SEQUENCE ALIGNMENT AND DATABASE S EARCHES L-13 Hours Introduction-The evolutionary basis of sequence alignment, the Modular Nature of Proteins.

Methods of sequence alignment: Pairwise (Global and Local Alignment) and Multiple Sequence Alignment (MSA). Progressive sequence alignment method, Position Specific Scoring Matrix (PSSM), DOT PLOT method. Optimal Alignment Methods- Dynamic Programming. Internet

based analysis tools- Clustal W and T-coffee. Practical issues of alignment, Profiles and Hidden Markov Model, Motif & Patterns. Database similarity searching: BLAST and FASTA, PSI-BLAST & PHI-BLAST, Low complexity regions, Repetitive Elements. Conceptual numericals.

Tutorials: Solving dynamic programming problems practices on multiple and pair wise sequence alignment tools

UNIT-3

PHYLOGENETIC ANALYS IS: L-13Hours Introduction, concepts of trees, phylogenetic trees and multiple alignments. Distance matrix

method (MD), character based methods, methods of evaluating phylogenesis, summary of the phylogenetic methods. Steps in constructing alignments and phylogenesis. Phylogenetic

softwares (CLUSTAL W, PHYLIP etc), PREDICTIVE METHODS Predictive Methods using Nucleotide sequences: Framework, Masking repetitive DNA, Database

searches, Codon Bias Detection, Detecting Functional Sites in the DNA (promoters, transcription factor binding sites, translation initiation sites), Integrated Gene Parsing, finding RNA Genes,

Web based tools (GENSCAN, GRAIL, GENEFINDER). Predictive Methods using Protein sequences: Protein Identity based on composition, Physical properties Based on sequence, secondary structure and folding classes, specialized structures or features, tertiary structure.

Related web based software (JPRED, PROSEC, NNPREDICT and SOPMA) Tutorials: Practices on use of phylogentic tools, gene finder tools gen scan tools NN predict

tools

UNIT- 4

PLASMID MAPPINGAND PRIMER DESIGNING & PROTEIN STRUCTURE

VISUALIZATION: L-13 Hours Restriction mapping Web based tools: Restriction Mapper, (MAP, REBASE) Utilities of DNA strider, Mac Vector and OMIGA, Gene construction KIT, Primer design – need for tools, Primer

design programs and software (PRIME3). Usages of visualization software available in public domain like RASMOL, SWISS PDB Viewer, chime pymol and Cn3D.

MOLECULAR MODELING, DRUG DES IGN AND DISCOVERY: Generation of Rational Approaches in Drug Design, molecular docking, quantitative structure-activity relationship (QSAR), Receptor Mapping, Estimating Biological Activities, Molecular Interactions: Docking,

Calculation of Molecular Properties, Energy Calculations (no derivation), Target identification, Target validation, Modeling, Virtual screening, lead identification, Lead Validation, and

Molecular Interactions. Tutorials: Problems on analysis of restriction mapping practices on restriction mapping tools analysis of structure visualization tools and practices on Insilco drug design tools.

TEXT BOOKS:

1. Bioinformatics – Andreas D Baxevanis. Wiley Interscience, 1998. 2. Bioinformatics –David W Mount, cold spring harbor, 2005.

REFERENCE BOOKS: 1. Introduction to Bioinformatics – Arthur Lesk, Oxford, 2006. 2. Bioinformatics – Stuart M Brown, NYU Medical Center, NY USA. 2000. 3. Fundamental Concepts of Bioinformatics – D E Krane & M L Raymer, Pearson, 2006. 4. Computational methods for macromolecular sequence analysis – R F Doolittle. academic Press, 1996. 5. Computational methods in Molecular Biology – S.L.Salzberg, D B Searls, S. Kasif, Elsevier, 1998.

6. Bioinformatics, Methods And Applications– Genomics, Proteomics And Drug discovery – s c

Rastogi, n mendiratta & p rastogi, phi, 2006. 7. The molecular modeling perspective in drug design – N Claude Cohen, 1996, Academic Press

8. Analytical Tools for DNA, Genes & Genomes: – Arseni Markoff, New Age, 2007.

COURS E OUTCOMES: 1. Able to understand the Databases and its types and tools.

2. Able to understand the sequence alignment and database searches

3. Able to know about phylogenetic analysis.

4. Able to understand Restriction mapping and Sequencing methods.

5. Able to know applications of insilico modeling in modern biology.

6. Able to study insilico drug design

Sub: Code Course

Outcomes

Programme Outcomes

Programme Speci fic

Outcomes

UBTUBT503C 1 2 3 4 5 6 7 8 9 10 11 12 PSO1

PSO2

PSO3 CO 1 3 2 - - 2 1 2 2 3 2 2 3

CO 2 3 2 2 2 2 1 2 - 3 2 2 3

CO 3 3 2 - 1 - - 2 - 3 2 2 3

CO 4 2 2 - 1 - 2 - - 3 1 - 2

CO 5 2 2 2 1 - 2 - 2 1 2 - 2

CO 6 2 1 2 2 2 2 1 1 1 1 1 1


1. CIE comprises of 3 tests, each of 30 marks and I hr duration,totaling to 90 marks and later is

scaled down to 45 marks


3. Any two full questions to be answered out of three questions and each question carries

fifteen marks


QUESTION PAPER PATTERN OF SEE

1. Total of Eight Question with Two from each unit to be set uniformly covering the entire syllabus. 2. Each question should not have more than four sub questions.

3. Each full question carries 20 marks 4. Any Five Full questions are to be answered choosing at least one from each unit.

UBT504C: GENETIC ENGINEERING & APPLICATIONS

4 Credits (4-0-0)

Prerequisites : Molecular Biology

UNIT- 1

INTRODUCTION L-13 Hours Role of genes within cells, genetic elements that control gene expression, Tools of genetic

engineering- vectors in recombinant DNA technology, biology and salient features of vectors, Types of vectors - plasmids, cosmids, phages and viruses.

ENZYMES IN GENETIC ENGINEERING Introduction- Restriction Endonucleases-classification, mode of action, applications. Enzymes used in nucleic acid modification – Alkaline phosphatase, Polynucleotide phosphorylase ,

DNase, polynucleotide Kinase, Ligases, RNase, terminal deoxy nucleotidyl transferase,S1 nuclease, T4 DNA polymerase, Poly A polymerase.

UNIT- 2 NUCLEIC ACID HYBRIDIZATION AND AMPLIFICATION L-13 Hours

Methods of nucleic acid detection, Fluorescent In situ hybridization(FISH), colony hybridization, polymerase chain reaction (PCR), its types and applications, methods of nucleic acid

hybridization- probe and target sequences, Southern, Western and Northern hybridization techniques. CONSTRUCTION OF DNA LIBRARIES

Isolation and purification of nucleic acids, quantification, Isolation of plasmids, Construction of cDNA, genomic and cDNA libraries, screening and preservation of libraries.

UNIT- 3

GENE TRANSFER TECHNIQUES L-13 Hours Gene transfer techniques in plants, animals and microbes –Transformation, microinjection,

electroporation, microprojectile system, and liposome mediated transfer, embryonic stem cell method. Agrobacterium-mediated gene transfer in plants – Ti & Ri Plasmid: structure and functions, Ti based vectors- Binary vectors and Cointegrate vectors.

TRANS GENIC SCIENCE AND GENETIC IMPROVEMENT

Transgenic science in plant improvement, biopharming – plants as bioreactors, transgenic crops

for increased yield, Antisense RNA technology (Flavr savr tomatoes). Application of plant transformation for productivity and performance – Herbicide resistance - glyphosate. insect resistance - Bt genes ( Bacillus thuringiensis and its mode of action), Cry proteins –

mechanism of action. Transgenic for disease resistance- coat protein mediated, PR proteins. Abiotic stress – drought and salinity.

UNIT- 4

GENE THERAPY L-13 Hours

Introduction, Methods of Gene therapy-gene targeting, gene augmentation, assisted killing, prodrug therapy and gene silencing. Gene therapy in the treatment of cancer, SCID, muscular

dystrophy, Use of genetically modified and humanized antibodies against cell surface antigens to prevent the spread of breast cancers and prevent organ graft rejection. Use of thrombolytic agents in blood clotting. Challenges in gene therapy.

OTHER APPLICATIONS Microbial biotechnology - Genetic manipulation, engineering microbes for the production of

Insulin, growth hormones, monoclonal antibodies. Total: 52 Hours

TEXT BOOKS

1. Molecular Biotechnology, Principles and applications of Recombinant DNA by Bernard R Glick and Jack J Pasternak, second edition, CBS Publishers, 2012

2. Recombinant DNA by Watson, et al., second edition, Freeman Publishers 2010.

REFERENCE BOOKS

Principles of gene manipulation, Primrose S.B., Blackwell Scientific Publications,

2010.

From Genetics to Gene Therapy – the molecular pathology of human disease by

David S Latchman, BIOS scientific publishers, 2010.

Biotechnology Expanding Horizon, B.D.Singh, 3rd revised edition, Kalyani

Publishers,2010

NPTEL Source material

COURS E OUTCOMES:

Understand analyze and apply the techniques in various fields of biotechnology.

Ability to salient features and applications of different enzymes in genetic

engineering.

Demonstrate the different strategies and approaches involved in gene cloning

Demonstrate the methodologies and applications of general PCR and variant PCR.

Ability to comprehend various techniques involved in cDNA.

Describe the different gene transfer techniques in prokaryotic and eukaryotic

cells.

Analyse and list the genetic engineering concepts involved in gene therapy and

therapeutic applications.

Course

Outcom

es

Programme Outcomes

Programme Speci fic

Outcomes

1 2 3 4 5 6 7 8 9 10 11 12 PSO1 PSO

2

PSO

3

CO 1 3 3 2 2 2 1

CO 2 3 3 2 3 2 1

CO 3 2 1

CO 4 3 3 2 3 3 2 1

CO 5 3 3 3 2 1

CO 6 3 2 2 3 2 1

CO 7 3 3 3 3 3 2 1


CIE comprises of 3 tests, each of 30 marks and I hr duration, totaling to 90 marks and later is scaled down to 45 marks

Each CIE Will be Covering Complete unit

Any two full questions to be answered out of three questions and each question

carries fifteen marks Assignment/ Quiz/objective tests carries five marks

QUESTION PAPER PATTERN OF SEE 1. Total of Eight Question with Two from each unit to be set uniformly covering the entire

syllabus. 2. Each question should not have more than four sub questions.

3. Each full question carries 20 marks 4. Any Five Full questions are to be answered choosing at least one from each unit.

UBT516C: BIOPROCESS & REACTION ENGINEERING

4 Credits (4-0-0)

Prerequisites : Unit Operations

UNIT- 1

Introduction L-10Hours T-3Hours

Law of mass action and rate equation, definitions and examples of elementary and non

elementary reactions, theories of reaction rate and temperature dependency, analysis of experimental reactor data: evaluation of rate equation, integral and deferential analysis for constant volume system. Conceptual numerical.

Biochemical Equilibria: Equilibrium in chemically reactive systems (single and multiple reactions), evaluation of reaction

equilibrium constant, effect of temperature on equilibrium- derivation of G vs. T relation, application of above concepts to biochemical systems. Conceptual numerical.

UNIT- 2

Bioreactors L-10Hours T-3Hours

Design equations for homogeneous systems: batch, stirred tank and tubular flow reactor, size comparison of reactor systems, combination reactor systems. Optimization of output and yield

problems, qualitative design for consecutive, parallel and mixed reactions. Factors affecting choice of reactors. Conceptual numerical. Non-Ideal Bioreactors:

Non-ideal reactors, residence time distribution studies, pulse and step input response of reactors, RTD’s for CSTR and PFR, calculations of conversions for first order reactions, tanks in series

and dispersion models. Conceptual numerical.

UNIT- 3

Kinetics of Microbial Growth and Product Formation: L-10Hours T-3Hours

Yields in cell culture, Cell growth kinetics: Batch growth, Balanced Growth: Monod equation,

Effects of substrate concentration, Production kinetics in cell culture: Directly/ indirectly coupled with energy metabolism, Not coupled with energy metabolism. Kinetics of substrate uptake in cell culture: in the absence/ in association of product formation.

Effect of culture conditions on cell Kinetics. Determining cell kinetic parameters from batch data, rates of growth, product formation and substrate uptake, cell doubling time μmax, and Ks

with with conceptual numerical. Effect of maintenance on yields. Observed yields, Biomass/ product yield from substrate, product yield from biomass, Leudeking-Piret models. Kinetics of cell death with conceptual numerical.

UNIT- 4

Thermodynamics of microbial growth L-10Hours T-3Hours

Energetic analysis of microbial growth and product formation, Heat of reaction with oxygen as/ not as principal electron acceptor. Energy balance equation for cell culture.

Transport Phenomena In Biochemical Reactors: Mass Transfer in heterogeneous biochemical reaction systems. Oxygen transfer in submerged fermentation processes; oxygen uptake rates and determination of oxygen transfer coefficients

(KLa); role of aeration and agitation in oxygen transfer. Total: 52Hours

TEXT BOOKS:

1. Bioprocess Engineering- Shuler and Kargi Prentice Hall, 1992.

2. Biochemical Engineering Fundamentals- Bailey and Olli’s, McGraw Hill (2nd Ed.) 1986

REFERENCE BOOKS:

Bioprocess Engineering- Aiba, Humprey & Millis, Academic Press.

Principles of Biochemistry – Leninger A.L.., II Edition, 1993.

Enzyme Kinetics- Plowman, McGraw Hill, 1972.

Chemical Engineering Kinetics- Smith J.M.., McGraw Hill, 3rd Edition, New Delhi, 1981.

COURS E OUTCOMES:

Define the types of reactions, evaluations of rate of reactions

Apply the applications of biochemical reactions, equilibrium constants

Derive the design equations for different types of bioreactors

Calculaate the RTD for different types of reactors

Evaluate the Kinetics of Microbial Growth and Product Formation

Explain the Kinetics of substrate uptake in cell culture

Find Energetic analysis of microbial growth and product formation,

Apply the Mass Transfer in heterogeneous biochemical reaction systems

Course

Outco

mes

Programme Outcomes

Programme Specific

Outcomes

1 2 3 4 5 6 7 8 9 10 11 12 PSO1 PSO2

PSO3

CO 1 3 2 2 2 1

CO 2 3 2 3 2 1

CO 3 3 2 2 3 2 1

CO 4 3 2 2 2 2 1

CO 5 3 3 3 2 1

CO 6 3 2 2 2 2 1

CO 7 3 3 2 2 1

CO 8 3 3 2 2 1

QUESTION PAPER PATTERN OF CIE (Continuous Internal Evaluation): 1. CIE comprises of 3 tests, each of 30 marks and I hr duration, totaling to 90 marks and later is

scaled down to 45 marks


3. Any two full questions to be answered out of three questions and each question carries

fifteen marks


QUESTION PAPER PATTERN OF SEE:

1. Total of Eight Question with two from each unit to be set uniformly covering the entire Syllabus. 2. Each Question should not have more than four sub questions.

3. Any Five questions are to be answered choosing at least one from each unit.

UBT506H: INDUSTRIAL SAFETY & BIOETHICS

3 Credits (3-0-0)

Prerequisites : Basic and applied aspects of life science

UNIT 1

Introduction to Bioethics & Biosafety: L-10 Hours Definition and scope of bioethics and biosafety, Ethical implications and need for biosafety, Legal and Socio-Economic impacts of Biotechnology. Convention on biological weapons.

Bioterrorism-classification of biological agents with examples. Biosafety regulation guidelines

Recombinant DNA Advisory Committee (RDAC) ,Institutional Biosafety committee(IBC),Review Committee on Genetic Modification (RCGM),Genetic Engineering Approval Committee(GEAC), Biosafety guidelines- national guidelines, Cartagena Protocol on

Biosafety.

UNIT 2

Biosafety Regulation: L-12 Hours Genetically modified organisms and their release in environment, Laboratory associated

infections and other hazards, Good Lab Practices and Good Manufacturing Process (GLP &GMP). Biosafety levels for microorganismBL1,BL2,BL3,BL4) plants (BL1-P,BL2-P,BL3-

P,BL4-P) animals (BL1-N,BL2-N,BL3-N,BL4-N). Risk assessment during laboratory research and risk groups. Recombinant organisms and transgenic crops. Guideline for labeling GM crops. Containments; Physical, Biological. Risk

management strategies for the release of transgenic plants. Field trial methods using transgenic plants. Animal cloning-ethics involved in animal cloning.

UNIT 3

Food and Pharma safety: L-10 hours The GM-food debate and biosafety assessment procedures for biotech foods & related products,

including transgenic food crops, case studies of relevance. Procedure to apply patent, Copy right, Plant Breeder’s Right, Environmental aspects of biotech applications. Special application of patent laws in biotechnology and case studies. Flavr Savr Tomato as model case, case studies of

relevance (Eg. Bt cotton, Bt brinjal). Licensing and cross licensing. Biosafety assessment of biotech pharmaceutical products.

UNIT4

Industrial safety:

Need for safety, importance of occupational safety, Health and safety programs, Safe and unsafe conditions.

Accidents: Accident preventive measure, Measurement and control of safety performance, 5E’s for accident prevention Safety policy Fire: Fire extinguishers and fire exits, extinguishing agents.

Importance of safety in food and Pharma industry. Food safety, Biological, chemical and Physical Hazards-HAACP system, Pharma safety. Food and safety act. Injuries by industrial

sector. Total: 42 Hours

TEXTBOOKS:

1. Bioethics and Biosafety by Sateesh M.K., I.K.International pub,2012 2. Biotechnology Expanding Horizon, B.D.Singh, 3rd revised edition, Kalyani

Publishers,2010

REFERENCE BOOKS:

Biotechnology and Safety Assessment by Thomas, J.A., Fuch, R.L. Academic Press.

2002.

Biological safety Principles and practices)by Fleming, D.A., Hunt, D.L., ASM Press2000

IPR-Biosafety and Bioethics Deepa Goel, Shomini Parashar, 2nd edition, Pearson Education India Publishers,2010

COURS E OUTCOMES:

Ability to Understand scope and aspects of Bioethics and biosafety,

Ability to understand ethical implications of biotechnology and need for biosafety.

Ability to understand biosafety regulation guidelines

Ability to understand analyze the public perception and public issues regarding

Biotechnology.

Ability to understand analyze safer use of Biotechnology in Agriculture, Animal

husbandry, Pharma, and Environment by implanting biosafety regulations.

Ability to understand and analyze market strategies, status for BT products, and to

understand the concept of IPR.

To Understand the importance of EHS concept of the industry

To understand the food and pharma industry safety

. Course

Outcom

es

Programme Outcomes

Programme Speci fic

Outcomes

1 2 3 4 5 6 7 8 9 10 11 12 PSO1 PSO2

PSO3

CO 1 3 1 3 3 1 2 1 2

CO 2 3 3 3 3 1 2 1 2

CO 3 1 3 3 1 2 1 2

CO 4 3 3 2 1 3 3 1 2 1 2

CO 5 3 3 1 3 3 1 2 1 2

CO 6 3 2 3 3 1 2 1 3

CO 7 3 3 1 3 3 1 2 1 3

CO 8 3 1 3 3 1 2 1 3

https://www.google.co.in/search?tbo=p&tbm=bks&q=inauthor:%22Deepa+Goel%22

https://www.google.co.in/search?tbo=p&tbm=bks&q=inauthor:%22Shomini+Parashar%22

QUESTION PAPER PATTERN OF CIE (Continuous Internal Evaluation): 1. CIE comprises of 3 tests, each of 30 marks and I hr duration, totaling to 90 marks and

later is scaled down to 45 marks. 2. Each CIE will be covering one complete unit


4. Assignment/quiz/ objective tests carries five mark


1. Total eight Question with Two from each unit to be set uniformly covering the entire syllabus. 2. Each Question should not have more than four sub questions.

3. Any Five Full questions to be answered choosing at least one from each unit

UBT521E: ENVIRONMENTAL BT

3 Credits (3-0-0)

Prerequisites : Microbiology, Environmental studies

UNIT- 1

MICROORGANISMS L-10 Hours Issues and scope of Environmental BT. Characteristics of soil, microbial flora of soil,

interactions among soil microorganisms, biogeochemical role of soil microorganisms. BIOACCUMULATION OF TOXICANTS

Characteristics of Xenobiotics, Relationship of Bioaccumulation with Chemical Structure, Ecophysiology of Bioaccumulation, Process of toxicants uptake, Factors affecting bioaccumulation, measurement of bioaccumulation.

UNIT- 2 BIOLOGICAL TREATMENT OF WASTE WATER L-12 Hours

Waste water characteristics BOD, COD, Primary & Secondary treatment, nanofiltration, ultafilration and microfiltration. Microbial removal of phosphorous and Nitrogen, Nutrient removal by Biomass production Wastewater treatment of food processing industries like sugar

factories, vegetable oil industries, potato processing industries, dairy industries, beverages industries, and distilleries.

SOLID WASTE MANAGEMENT Basic aspects, general composition of urban solid wastes, aerobic treatment, anaerobic treatment, biogas generation; Solid waste management through Biotechnological processes involving

Hazardous wastes, Biomedical wastes MoEF rules.

UNIT- 3

BIOLEACHING & BIOMINING L-10 Hours Microbes in Bioleaching- types, methods of bioleaching, Microbial recovery of metal,

phosphate, petroleum. BIOREMEDIATION: Major contaminants of air, water and soil, Biomonitors of environment (Bioindicators),

Bioremediation using microbes, Phytoremediation, Biofilms its applications. Bio-stimulation of Naturally occurring microbial activities, Bio-augmentation.

UNIT- 4

BIOTECHNOLOGY IN BIODIVERSITY CONS ERVATION L-10 Hours

Value of biodiversity, threats to biodiversity, Biosphere reserves and Ecosystem Conservation, Approaches to Bioresource conservation programme, Biotechnological processes for bioresource

assessment, BT in ex situ conservation of Biodiversity, BT and its role in utilization of Biodiversity, International initiatives for biodiversity management.

Total: 42 Hours

TEXT BOOKS:

Environmental Biotechnology by Pradipta Kumar Mahopatra.

Text book of microbiology by R C Dubey and D K Maheshwari

REFERENCE BOOKS:

Environmental Biotechnology by Foster C.F., John ware D.A., Ellis Horwood Limited,1987.

Bioprocess Technology- fundamentals and applications, S O Enfors & L Hagstrom (1992), RIT,.

Comprehensive Biotechnology Vol. 1- 4 : M.Y. Young (Eds.), Pergamon Press.

Industrial Microbiology : L.E. Casida, Willey Eastern Ltd., 1989.

Industrial Microbiology : Prescott & Dunn, CBS Publishers, 1987.

Biotechnology, Economic & Social Aspects : E.J. Dasilva, C Ratledge & A Sasson,

Cambridge Univ. Press, Cambridge.

COURS E OUTCOMES:

Students will be able to

1. Understand issues and scope of Environmental BT.

2. Explain Bioaccumulation process. 3. Develop different treatment methods to solve the environmental issues by using BT

approach.

4. Apply the knowledge of bioleaching for metal recovery. 5. Make use of bioremediation processes to remove environmental contaminants.

6. Understand the Value of biodiversity and threats to biodiversity. 7. Apply the knowledge of BT in biodiversity conservation.


CIE comprises of 3 tests, each of 30 marks and I hr duration,totaling to 90 marks and

later is scaled down to 45 marks

Each CIE Will be Covering Complete unit

Any two full questions to be answered out of three questions and each question carries fifteen marks

Assignment/ Quiz/objective tests carries five marks

Course Outcomes

Programme Outcomes


1 2 3 4 5 6 7 8 9 1

0

11 1

2

PSO1 PSO2 PSO3

CO 1 2 2 3 1 2

CO 2 2 3 2 2 3 1

CO 3 2 3 2 1 1 2 3 3 1

CO 4 1 2 2 3 2 2 1 3

CO 5 1 3 2 2 2 3 2 3

CO 6 2 3 3

CO 7 1 3 2 2 2 2 1 3

QUESTION PAPER PATTERN OF SEE: 1. Total eight Question with Two from each unit to be set uniformly covering the entire

syllabus. 2. Each Question should not have more than four sub questions.

3. Any Five Full questions to be answered choosing at least one from each unit.

UBT522E: BIOMEDICAL INSTRUMENTATION

3 Credits (3-0-0)

Prerequisites : Microbiology, instrumentation

UNIT- 1

INTRODUCTION 1 L-10 Hours Sources of Biomedical signals, Basic medical instrumentation system, Performance requirements

of medical instrumentation systems, PC based medical instruments, General constraints in design of medical instrumentation systems. 4 Hours UNIT 2. BIOELECTRIC SIGNALS AND

ELECTRODE Origin of bioelectric signals, Recording electrodes, - Electrode-tissue interface, metal electrolyte interface, electrolyte -skin interface, Polarization, Skin contact impedance, Silver – silver

chloride electrodes, Electrodes for ECG, EEG, EMG, Electrical conductivity of electrode jellies and creams, Microelectrode. Patient Safety: Electrode shock hazards, Leakage currents.

UNIT- 2

ECG & EEG L-10 Hours

Electrical activity of heart, Genesis & characteristics of Electrocardiogram (ECG), Block diagram description of an Electrocardiograph, ECG Lead Systems, Multichannel ECG machine

Genesis of Electroencephalogram (EEG), Block diagram description of an Electroencephalograph, 10-20 Electrode system, Computerized analysis of EEG. CARDIAC PACEMAKERS AND DEFIBRILLATORS

Need for Cardiac pacemaker, External pacemaker, Implantable pacemaker, Programmable pacemakers, DC defibrillator, AC defibrillator and Implantable Defibrillator.

UNIT- 3

PATIENT MONITORING SYSTEM L-10 Hours Bedside monitors, Central Monitoring System, Measurement of Heart rate -Average heart rate

meter, Instantaneous heart rate meter, (Cardio tachometer), Measurement of Pulse Rate, Blood pressure measurement -direct and indirect method, Rheographic method, Oscillometric method, Ultrasonic Doppler shift method, Measurements of Respiration rate -Thermistor method,

impedance puenmography, CO2 method, and Apnea detector. Blood flow meters: Electromagnetic and its types, Ultrasonic, NMR, Laser Doppler. Blood gas analyzers: Blood pH

measurement, Measurement of Blood pCO2, pO2. PHYS IOLOGICAL TRANSDUCERS Introduction, classification, performance characteristics of transducers-static and dynamic

transducers, Displacement, position and motion transducers, Pressure transducer, Transducers for body temperature measurement, Optical Fiber sensor and Biosensor

UNIT- 4

RECORDING SYSTEMS L-10 Hours

Basic recoding system, general considerations for signal conditioners, preamplifiers-instrumentation amplifier, isolation amplifier, ink jet recorder, potentiometric recorder, thermal

array recorder and electrostatic recorder. Analysis of Cardiac output measurement: Indicator dilution method, Dye dilution method, Thermal dilution techniques, Measurement of Continuous cardiac output derived from the aortic

pressure waveform, Impedance technique. Pulmonary function analysis: Pulmonary function measurement, Spirometry, Puemotachometer, Measurement of Volume, Nitrogen washout

technique. Total: 40 Hours

TEXT BOOKS

1. Hand book of Biomedical Instrumentation – R. S. Khandpur, 2nd Edition, Tata McGraw-Hill Publishing Company Limited, 2003.

2. Introduction to Biomedical Engineering by J Enderle, S Blanchard & J Bronzino, Elsevier, 2005.

REFERENCE BOOKS

Encyclopedia of Medical devices and Instrumentation – J G Webster, John Wiley, 1999.

Principals of applied Biomedical instrumentation – John Wiley and sons,2000

3.Introduction to Biomedical equipment technology – Joseph J Carr, John M Brown Prentice hall 4th Edition,2005

COURS E OUTCOMES:

Able to understand basic concepts of biomedical signals.

Able to know ECG and EEG.

Able to understand the patient monitoring system and recording systems

Able to know characteristics of transducers

Able to understand various analysis techniques

Able to understand the recording systems.

Course Outcomes

Programme Outcomes


1 2 3 4 5 6 7 8 9 1

0

11 1

2

PSO1 PSO2 PSO3

CO 1 2 1 2

CO 2 2 3 2 2 3 1

CO 3 2 3 2 1 3 3 1

CO 4 1 2 2 3 1 3

CO 5 1 3 2 2 2 3 1

CO 6 3

CO 7 1 3 2 2 1 3


CIE comprises of 3 tests, each of 30 marks and I hr duration,totaling to 90 marks and later is

scaled down to 45 marks.

Each CIE will be covering one complete unit

Any two full questions to be answered out of three questions and each question carries fifteen marks

Assignment/quiz/ objective tests carries five marks


Total eight Question with Two from each unit to be set uniformly covering the entire

syllabus.

Each Question should not have more than four sub questions.

Any Five Full questions to be answered choosing at least one from each unit.

UBT523E OPERATING S YSTEMS AND DBMS

3 Credits (3-0-0)

Prerequisites : Bioinformatics UNIT- 1

INTRODUCTION L - 10 Hours What is O.S, Von-Neumann architechture, Supercomputers, Mainframe systems, Desktop system, Multiprocessor systems, Distributor systems, Clustered systems, Real time systems,

Hand held systems, Future migration, Computing environment, System components, services, System calls, System programs, system structure, OS design and implementation, microkernels,

virtual machines

PROCESS MANAGEMENT Process concept, process state, process control block, process scheduling, snail diagrams,

schedulers, creation and removal of a process, interprocess communication, models for IPC, independent and cooperating processes, threads, overview, multithreading, applications, critical

selection problem, Semaphores, deadlocks and starvation.

UNIT- 2

STORAGE MANAGEMENT L - 10 Hours

Memory management, dynamic loading and linking, overlays, logical vs physical space,

memory management unit, swapping, contigous allocation, fragmentation, paging, page table, segmentation, virtual memory, demand paging, thrashing file system, interface-file concept, directory implementation.

LINUX AND WIN NT Linux: Design principles, Kernel modules, process management, scheduling, memory

management systems, input and output, interprocess communication. WinNT: Design principles, system components, environmental subsystems, file system, networking and programming interface

UNIT- 3

DESIGN OF DBMS L - 10 Hours Introduction to DBMS, terminology, Systems Development Life Cycle, terms of reference,

feasibility report, data flow diagrams, addition of data sources, identification of individual processes, inputs and outputs, system boundaries, Entity-Relationship modeling, examples,

database creation using MS Access, designing tables using Access, Data Integrity, Normalization, relationships between tables, comparing E-R design with Normalization design, Inclusion of new requirements from feasibility report, documentation, amending primary keys

and database tables, Practical examples. DATA DICTIONARY AND QUERY DES IGN

Data dictionary, criteria, compiling a list of field names, entry sequence for the table data, entering, sorting and filtering of data in a table, introduction to queries, identifying field names, selection criteria and sort order in a query, calculations in queries, modifying a query, creating a

query using design view and wizard in MS Access

Formatted: C entered


UNIT- 4

REPORTING, TESTING AND DOCUMENTATION L - 10 Hours

Introduction to reporting, dataflow diagram based reporting and table based reporting, form creation using wizard, entering and searching records in a form, modifying forms and reports,

Introduction to testing, types (unit testing, system testing, integration testing, interface testing, performance testing and user testing), test data, executing and error reporting, introduction to documentation, areas of documentation

SETTING UP THE DATA AND HOUS EKEEPING Approaches to set up data (parallel, bigbang, phased and pilot implementation), working data,

data entry methods to the database (systems screen, external source), introduction to housekeeping, regular backups, archiving old data, maintaining security in a database.

TEXT BOOKS

1. Mastering Database Design by Helen Holding, Macmillan publications, 1999.

2. Operating system concept by Silberschatz, peterhalvin and Greg Gague, VI edition, John Wiley, 2003.

REFERENCE BOOKS

Linux: the complete reference by Richard Peterson, McGraw Hill, 1998

Operating System – A concept based approach by D Dhamdene, Tata McGraw Hill, 2002.

COURS E OUTCOMES:

Able to understand basic concepts of OS design and implementation.

Able to analyse interprocess communication .

Able to understand the Memory management.

Able to know the concept of LINUX

Able to understand various DBMS designs

Able to understand the concepts of reporting, testing and documentation.

Course

Outcom

es

Programme Outcomes

Programme Speci fic

Outcomes

1 2 3 4 5 6 7 8 9 10 11 12 PSO1 PSO2 PSO3

CO 1 3 2 2

2 1

CO 2 3 2 3

2 1

CO 3 3 2 2 3

2 1

CO 4 3 2 2 2

2 1


Formatted: F ont: 10 pt

Formatted: F ont: T imes New Roman

Formatted: F ont: T imes New Roman, NotBold, F ont color: A uto





Formatted: F ont: 10 pt, Not Bold, F ont color:A uto















CO 5 3 3 3

2 1

CO 6 3 2 2 2

2 1

CO 7 3 3 2

2 1

CO 8 3 3 2

2 1

QUESTION PAPER PATTERN OF CIE (Continuous Internal Evaluation): 1. CIE comprises of 3 tests, each of 30 marks and I hr duration,totaling to 90 marks and

later is scaled down to 45 marks. 2. Each CIE will be covering one complete unit


4. Assignment/quiz/ objective tests carries five marks


1. Total eight Question with Two from each unit to be set uniformly covering the entire syllabus.

2. Each Question should not have more than four sub questions.

3. Any Five Full questions to be answered choosing at least one from each unit.

















UBT525E: STEM CELL TECHNOLOGY

Credits 3(3-0-0)

Prerequisites : , Genetic engineering and applications UNIT- I

STEM CELLS AND CELLULAR PEDIGREES L – 10Hours

Scope of stem cells – definition of stem cells – concepts of stem cells – differentiation , maturation , proliferation , pluripolericy, self – maintainance and self – renewal –problems

in measuring stem cells – preservation protocols.

UNIT- II

STEM CELL CONCEPT IN PLANTS L - 10Hours Stem cell and founder zones in plants – particularly their roots – stem cells of shoot meristems of higher plants.

UNIT- III

STEM CELL CONCEPT IN ANIMALS L - 10Hours Skeletal muscle stem cell – Mammary stem cells – intestinal stem cells – keratinocyte stem cells

of cornea – skin and hair follicles –Tumour stem cells, Embryonic stem cell biology - factors influencing proliferation and differentiation of stem cells – hormone role in differentiation.

UNIT- IV

HAEMOPOIETIC STEM CELL L - 10Hours

Biology – growth factors and the regulation of haemopoietic stem cells. POTENTIAL US ES OF STEM CELLS

Cellular therapies – vaccines – gene therapy – immunotherapy – tissue engineering –blood and bone marrow – Fc cells.

TOTAL : 40 Hours

TEXT BOOKS

J. J. Mao, G. Vunjak-Novakovic et al (Ed): Translational Approaches in Tissue Engineering & Regenerative Medicine 2008, Artech House, INC Publications.

Robert Lanza et al. Principles of Tissue Engineering, 3rd Edition. Academic Press; 3 edition (August 21, 2007)

REFERENCE BOOKS

1. Stein et al. Human Stem Cell Technology and Biology: A Research Guide and Laboratory Manual.Wiley-Blackwell; 1 edition (January 4, 2011)

2. Lanza et al. Handbook of Stem Cells, Two-Volume Set: Volume 1-Embryonic Stem Cells; Volume 2-Adult & Fetal Stem Cells (v. 1).Academic Press (September 28, 2004)

COURS E OUTCOMES:

Students will be able to

1. Isolate and Culture of Hematopoietic Stem cells 2. Isolate and Culture of Mesenchymal Stem cells

3. Analyse Differentiation of Pluripotent stem cells 4. Interpret Cell culture in Scaffolds 5.Anayse growth of haemopoietic stem cells

6. Apply the potential uses of stem cells


CIE comprises of 3 tests, each of 30 marks and I hr duration,totaling to 90 marks and later is scaled down to 45 marks.

Each CIE will be covering one complete unit

Any two full questions to be answered out of three questions and each question carries

fifteen marks

Assignment/quiz/ objective tests carries five marks


Total eight Question with Two from each unit to be set uniformly covering the entire syllabus.

Each Question should not have more than four sub questions.

Any Five Full questions to be answered choosing at least one from each unit.

Course

Outcomes

Programme Outcomes

Programme Specific

Outcomes 1 2 3 4 5 6 7 8 9 1

0 11 1

2 PSO1 PSO2 PSO3

CO 1 1 1 2 2 2

CO 2 2 3 2 1 1 2 3 1

CO 3 2 3 2 1 1 3 3 1

CO 4 1 2 2 1 2 1 3

CO 5 1 3 2 2 1 2 3 1

CO 6 1 3 2 2 2

UBT511L -BIOSTATISTICS LAB

1 Credits (0-0-2)

LIST OF EXPERIMENTS

1. Procedure for creating Data file, Diagram and Graphs. 2. Procedure and calculation of Mean, Median, Mode, Standard Deviation and Variance. 3. Procedure and calculation of t, Z and F test.

4. Calculation of Chi-square test. 5. ANOVA- one-way analysis

6. ANOVA- two-way analysis. 7. Experimental Research Design – CRD- Analysis. 8. Experimental Research design – RBD- Analysis.

9. Experimental Research design – Latin square- Analysis. 10. Calculation of Regression and correlation.

11. Multiple Regression Analysis. 12. Placket-Burman Design for media optimization. 13. Response Surface Methodology for media optimization.

COURS E OUTCOMES:

1. Will be able to draw graphs, charts, enter the data using statistical software tools 2. Will be able to calculate measures of dispersion and central tendency 3. Will be to do analysis of t, z and f test

4. Will be able to solve and analyze ANOVA 5. Will know different types of experimental designs with case studies

6. Will be aware of media optimization techniques using statistical designs

Course

Outcom

es

Programme Outcomes

Programme Speci fic

Outcomes

1 2 3 4 5 6 7 8 9 10 11 12 PSO1 PSO2 PSO3

CO 1 3 3 1 3

1 2 2 1 1

CO 2 3 3 2 3 3

2 2 2 1

CO 3 2 3 3 2 2 2

3 2 1

CO 4 3 3 1 3 3 2

3 2 1 2

CO 5 2 3 1 3 3 1 2 2 1 1





















CO 6 1 3 1 3 2

2 2 1 1




REFERENCE BOOKS: 1. Marcello Pagano & Kimberlee Gauvreu, “Principles of Biostatistics” -Thompson

Learning Pub, 2006. 2. Ronald N Forthofer and Eun Sul Lee, “Introduction to Biostatistics” -Academic Press,

1995. 3. Agarwal B L., “Basic Statistics”-New Age International Pub, fifth Edn, 2009 4. Norman T J Bailey, “Statistical methods in Biology” -Cambridge Press, 1995.

5. Kapur J N., “Mathematical Models in Biology and Medicine”, 1st Edition, New age international Pvt. Ltd, 2001.

6. Khan and Khanum, Fundamentals of Biostatistics, Ukaaz pub,3 rd edn,2008 7. Rubinow S I., “Introduction to Mathematical Biology” -John Wiley, 1975. 8. Richard A. Johnson, “Miller & Freund’s Probability and statistics for engineers” Prentice

Hall, 2000 9. Veer Bala Rastogi, “Fundamentals of Biostatistics” -Ane Books, 2006

10. Sabine Landau and Brian S. Everitt, A Hand Book of Statistical Analysis using SPSS, Chapman & Hall/CRC.

LABORATORY ASSESSMENT

1) Each laboratory subject is evaluated for 100 marks (50 CIE and 50 SEE)

2) Allocation of 50 marks for CIE

Performance and Journal write-up: marks for each experiment = 30 marks/No. of proposed experiments.

One practical test, for 20 marks (5 write-up, 10 conduction, calculation, Result etc., 5 –viva-voce)

3) Allocation of 50 marks for SEE, Major and Minor : 35 (Write-up 25%, conduction 50%, calculation and results 25%)

Spotting : 08 Viva-Voce : 07

UBT508L: BIOINFORMATICS LAB

1.5 Credits (0-0-3)

LIST OF EXPERIMENTS IN BIOINFORMATICS LABORATORY

Bibliographic search from PUBMED, SCIRUS and MEDMINER

Sequence retrieval from Nucleic acid and Protein databases.

Sequence searches using BLAST – Retrieval of homologs, paralogs, orthologs, and Xenologs

Pair wise comparison of sequences – Analysis of parameters affecting alignment.

Multiple alignments of sequences and pattern determination using PROSITE

Evolutionary studies / Phylogenetic analysis – Analysis of parameters affecting trees.

Identification of functional sites in Genes / Genomes.

Secondary structure prediction of proteins and comparison with PDB.

Restriction mapping: Analysis of maps for suitable molecular biology experiment.

Primer Design: Factors affecting primer design.

PDB structure retrieval and visualization: Analysis of homologous structures.

Determination of ligand-protein interactions using SPDBV/ LIGPLOT

Superposition of structures – Calculation of RMSD.

Docking studies – Analysis of substrate / ligand binding using homologous structures.

REFERENCE BOOKS

1. Bioinformatics – Andreas D Boxevanis. Wiley Interscience, 1998. 2. Bioinformatics – David W Mount, cold spring harbor, 2001.

3. Bioinformatics – A biologists guide to biocomputing and the internet. Stuart M brown, NYU Medical Center, NY USA. 2000. 4. Analytical Tools for DNA, Genes & Genomes – Arseni Markoff, New Age, 2007.

5. Discovering Genomics, Proteomics & Bioinformatics – A M Campbell & L J Heyer, Pearson Education, 2007

6. Fundamental Concepts of Bioinformatics – D E Krane & M L Raymer, Pearson, 2006. 7. Computational methods in Molecular Biology – S.L.Salzberg, D B Searls, S Kasif, Elsevier, 1998.

8. Bioinformatics – methods and applications: Genomics, proteomics and drug Discovery – s c Rastogi, N. mendiratta & prastogi, phi, 2006

COURS E OUTCOMES

Ability to Search literature and sequence databases

Ability to retrieve and search sequences from databases

Ability to align pair wise and multiple sequences

Ability to identify evolutionary and relationships and functional sites in genomes

Ability to evaluate primer designing and restriction mapping

Ability to docking and superimpose the structures

Sub: Code Course

Outcomes

Programme Outcomes

Programme Speci fic

Outcomes

UBT622E 1 2 3 4 5 6 7 8 9 10 11 12 PSO1 PSO2 PSO3

CO 1 3 3 3 - 3 1 - 3 3 3 3 1

CO 2 3 3 3 - 3 1 - - 3 2 3 1

CO 3 3 3 2 2 3 1 1 - 3 3 3 1

CO 4 3 3 2 - 3 - 1 - 3 2 3 2

CO 5 3 3 2 1 3 1 - 2 3 3 3 2

CO 6 3 3 3 2 3 1 - 1 3 2 3 1

LABORATORY ASSESSMENT 1) Each laboratory subject is evaluated for 100 marks (50 CIE and 50 SEE)

2) Allocation of 50 marks for CIE

Performance and Journal write-up: marks for each experiment = 30 marks/No. of

proposed experiments.

One practical test, for 20 marks (5 write-up, 10 conduction, calculation, Result etc., 5 –

viva-voce) 3) Allocation of 50 marks for SEE,

Major and Minor : 35

(Write-up 25%, conduction 50%, calculation and results 25%) Spotting : 08

Viva-Voce : 07

UBT509L: GENETIC ENGINEERING LABORATORY

UBT509L: GENETIC ENGINEERING LABORATORY

1.5 Credits (0-0-3)

LIST OF EXPERIMENTS IN GENETIC ENGINEERING LABORATORY

1. Transformation.

2. Blue white colony screening.

3. Thermal denaturation of DNA.

4. Restriction Digestion.

5. Ligation Experiment.

6. Southern Blotting – Agarose Gel Electrophoresis

7. Electroblotting and analysis.

8. SOP for PCR

9. SOP for Gel Documentation

10. SOP for UV-Spectrophotometer

11. SOP for Lyophilizer

12. PCR (Amplification with specific primers)

REFERENCE BOOKS 1. Principles of Gene manipulation-An introduction to genetic engineering, Old

R.W.,PrimroseS.B.,Blackwell Scientific Publications,1993. 2. Genetic Engineering Vol.1-4(Williamson Edition) 3. Current protocols in molecular biology-Greena Publishing Associates,NY,1988

4. Molecular cloning Volumes I,II,III-Sambrook J et al (2000).Cold Spring Harbor Lab, 2000.

5. Introduction to Genetic Engineering-Sandhya Nair

COURS E OUTCOMES:

To demonstrate proficiency in Transformation and screening of transformants.

To apply the knowledge of thermal denaturation to calculate Tm value.

To evaluate the functions of restriction digestion and Ligation on DNA.

To demonstrate proficiency in Electroblotting and detection.

To demonstrate understanding of SOP and PCR.

To gain knowledge in common and advanced laboratory practices in Genetic engineering

lab.

Course

Outcom

es

Programme Outcomes

Programme Speci fic

Outcomes

1 2 3 4 5 6 7 8 9 10 11 12 PSO1 PSO2 PSO3

CO 1 1 3 3 2 2 2 1 2

CO 2 3 3 2 3 2 1 1

CO 3 2 1 2

CO 4 1 3 3 2 3 3 2 1

CO 5 1 3 3 3 2 1 1

CO 6 2 3 2 2 3 2 1 1

LABORATORY ASSESSMENT 1) Each laboratory subject is evaluated for 100 marks (50 CIE and 50

SEE) 2) Allocation of 50 marks for CIE

Performance and Journal write-up: marks for each experiment = 30 marks/No. of proposed experiments.

One practical test, for 20 marks (5 write-up, 10 conduction, calculation, Result etc., 5 –viva-voce)

3) Allocation of 50 marks for SEE,

Major and Minor : 35 (Write-up 25%, conduction 50%, calculation and results 25%)

Spotting : 08 Viva-Voce : 07

SCHEME OF TEACHING AND EXAMINATION B.E. V SEMESTER … 2018-19.pdf · U.Satyanarayana and U....

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