Group Leader

Daniela Corda, Head of Laboratory of Cell Regulation,Department of Cell Biology and OncologyDirector, R&DConsorzio Mario Negri SudTel. +39 0872.570353; Fax. +39 0872.570412; e-mail: corda@negrisud.it

Group members

Stefania Mariggiò (Staff Scientists)Tel: 0872 570347/314; e-mail: mariggio@negrisud.it Guiling Li (Post-doc)Tel: 0872 570347/314; e-mail: li@negrisud.it Inma Ayala (Post-doc)Tel: 0872 570314; e-mail: ayala@negrisud.it Giovanna Grimaldi (PhD student)Tel: 0872 570352; e-mail: grimaldi@negrisud.it Alessia Varone (PhD student)e-mail: varone@negrisud.it Carmen Valente (PhD student)Tel: 0872 570347/314; e-mail: valente@negrisud.it Pasquale Zizza (PhD student)Tel: 0872 570314; e-mail: zizza@negrisud.it Cristiano Iurisci (Research Assistants)Tel: 0872 570314; e-mail: iurisci@negrisud.it

Key words (max 5)!S!i!g!n!a!l! !T!r!a!n!s!d!u!c!t!i!o!n; l!i!p!i!d!-!d!e!r!i!v!e!d! !s!e!c!o!n!d! !m!e!s!s!e!n!g!e!r!s; m!o!n!o!-!A!D!P!-!r!i!b!o!s!y!l!a!t!i!o!n!;!intracellular m!e!m!b!r!a!n!e! !transport; membrane f!i!s!s!i!o!n!.

Research projects

• Molecular mechanisms involved in the formation of intracellular membrane carriers: role ofthe CtBP/BARS protein-driven fissioning machinery.

• The CtBP/BARS involvement in tumor progression: a specific target for pharmacologicalintervention (AIRC)

• Molecular mechanisms and functional role of the mono-ADP-ribosylation of cellularproteins.

• Mechanism of of cell entry and of action of viral and bacterial toxins (Novartis Vaccines andDiagnostics)

• The phosphoinosite metabolites glycerophosphoinositols as regulators of actin cytoskeletonorganization (and tumor invasion) (MIUR).

• Analysis of the functional roles and molecular mechanisms of the glycerophosphoinositols inthe immune response.

• Lipid droplets as dynamic organelles of fat deposition and release: Translational researchtowards human disease. High throughput analysis of lipids and lipid-protein interactions(European Commission -Directorate Health)

• Molecular Determinants of Sterol-Sphingolipid-Protein Interactions in Living Cells andOrganisms (ESF –EuroMEMBRANES)

1. Hidalgo Carcedo C., Bonazzi M., Spanò S., Turacchio G., Colanzi A., Luini A. and CordaD. Mitotic Golgi partitioning is driven by the membrane fissioning protein CtBP3/BARS.Science 305: 93-96 (2004)

2. Bertini R., Allegretti M., Bizzarri C., Moriconi A., Locati M., Zampella G., CervelleraM.N., Di Cioccio V., Cesta M.C., Galliera E., Martinez F.O., Di Bitondo R., Troiani G.,Sabbatini V., Anacardio R., Cutrin J.C., Cavalieri B., Mainiero F., Strippoli R., Villa P., DiGirolamo M., Martin F., Gentile M., Santoni A., Corda D., Ghezzi P., Poli G., MantovaniA. and Colotta F. A new class of non-competitive allosteric inhibitors of the inflammatorychemokine receptors CXCR1 and CXCR2: prevention of reperfusion injury. Proc. Natl.Acad. Sci. USA 101: 11791-11796 (2004)

3. Mironov A.A., Colanzi A., Polishchuk R.S., Beznoussenko G.V., Mironov A.A. Jr., FusellaA., Di Tullio G., Silletta M.G., Corda D., De Matteis M.A. and Luini A. Dicumarol, aninhibitor of ADP-ribosylation of CtBP3/BARS, fragments Golgi non-compact tubular zonesand inhibits intra-Golgi transport. Eur. J. Cell Biol. 83: 263-279 (2004)

4. Dragani L.K., Berrie C.P., Corda D., Rotilio D. Analysis of glycerophosphoinositols byliquid chromatography-electrospray ionisation tandem mass spectrometry using aβ-cyclodextrin-bonded column. J. Chromatogr. B 802: 283-289 (2004)

5. Buccione R., Baldassarre M., Trapani V., Catalano C., Pompeo A., Brancaccio A., GiavazziR., Luini A. and Corda D. Glycerophosphoinositols inhibit the ability of tumor cells toinvade the extracellular matrix. Eur. J. Cancer 41: 470-476 (2005)

6. Bonazzi M., Spanò S., Turacchio G., Cericola C., Valente C., Colanzi A., Kweon H.-S., HsuV. W., Polishchuck E.V., Sallese M., Pulvirenti T., Corda D. and Luini A.  CtBP3/BARSdrives membrane fission at dynamin-independent trafficking pathways. Nat. Cell Biol. 7:570-580 (2005)

7. Bruno A., Rossi C., Marcolongo G., Di Lena A., Venzo A., Berrie C.P. and Corda D.Selective in vivo anti-inflammatory action of the galactolipid monogalactosyldiacylglycerol.Eur. J. Pharmacol. 524: 159-168 (2005)

8. Yang J-S., Lee S. Y., Spanò S., Gad H., Zhang L., Bonazzi M., Corda D., Luini A. and HsuV.W. BARS participates in the fission of COPI vesicles from Golgi membrane. EMBO J.24: 4133-4143 (2005)

Publications (last 5 years)

Collaborations

Martino Bolognesi, Dip. di Scienze Biomolecolari e Biotecnologie, Università di Milano;Giovanni Nicolosi, Istituto di Chimica Biomolecolare, CNR, Catania;Cosima T. Baldari, Dipartimento di Biologia Evolutiva, Università di Siena;Piero Pucci, Dipartimento di Chimica Organica e Biologica, Università di Napoli "FEDERICO II";Carlo Scolastico, Centro di Eccellenza CISI, Università di Milano;Rino Rappuoli, Novartis V&D, Siena;Mariagrazia Pizza, Novartis V&D, Siena;Paul O. Neilsen, Director of Research and Development, Echelon Biosciences Inc., USA;Patrizia Stoppelli, CNR, Institute of Genetics and Biophysics"A. Buzzati Traverso", Napoli;Elina Ikonen, Institute of Biomedicine, University of Helsinki, University of Helsinki, Finland;Antonio De Flora, DIMES Sezione Biochimica Università di Genova;María I. Colombo, Universidad Nacional de Cuyo, Mendoza-Argentina;Tommy Nilsson, RI-MUHC/McGill University, Montreal, Quebec;Luigi Bignardi, Facoltà di Ingegneria, Università de L’Aquila.

9. Di Girolamo M., Dani N., Stilla A. and Corda D. Physiological relevance of theendogenous mono-ADP-ribosylation of cellular proteins. FEBS J. 272: 4565-4575 (2005)

10. Valente C., Spanò S., Luini A. and Corda D. Purification and functional properties of themembrane fissioning protein CtBP/BARS. Methods Enzymol. 404: 296-316 (2005)

11. Corda D., Colanzi A., Luini A. The multiple activities of CtBP/BARS proteins: the Golgiview. Trends Cell Biol. 16: 167-173 (2006)

12. Nardini M., Svergun D., Konarev P. V., Spanò S., Fasano M., Bracco C., Pesce A.,Donadini A., Cericola C., Luini A., Corda D. and Bolognesi M. The C-terminal domain ofthe transcriptional co-repressor CtBP is intrinsically unstructured. Protein Sci. 15: 1042-1050 (2006)

13. Verger A., Quinlan K.G.R., Spanò S., Corda D., Kable E.P., Braet F., Crossley M.Mechanisms directing the nuclear localization of the CtBP family proteins. Mol. Cell Biol.26: 4882-4894 (2006)

14. Mariggiò S., Sebastià J., Filippi B., Iurisci C., Volontè C., Amadio S., De Falco V., SantoroM. and Corda D. A novel pathway of cell-growth regulation mediated by a PLA2α-derivedphosphoinositide metabolite. FASEB J. 20: 2567-2569 (2006)

15. Quinlan K.G. R., Nardini M., Verger A., Francescato P., Yaswen P., Corda D., BolognesiM. and Crossley M.. Specific recognition of ZNF217 and other zinc-finger proteins at asurface groove of CtBPs. Mol. Cell. Biol. 26: 8159-8172 (2006)

16. Mariggiò S., Bavec A., Natale E., Zizza P., Salmona M., Corda D. and Di Girolamo M.Gα13 mediates activation of the cytosolic phospholipase A2α through fine regulation of ERKphosphorylation. Cell. Signal. 18: 2200-2208 (2006)

17. Mariggiò S., Iurisci C., Sebastia J., Patton-Vogt J. and Corda D. Molecular characterizationof a glycerophosphoinositol transporter in mammalian cells. FEBS Lett. 580: 6789-96(2006).

18. Colanzi A., Carcedo C.H., Persico A., Cericola C., Turacchio G., Bonazzi M., Luini A. andCorda D. The Golgi mitotic checkpoint is controlled by BARS-dependent fission of theGolgi ribbon into separate stacks in G2. EMBO J. 26: 2465-2476 (2007)

19. Patrussi L., Mariggiò S., Paccani S.R., Capitani N., Zizza P., Corda D. and Baldari C.T.Glycerophosphoinositol-4-phosphate enhances SDF-1alpha-stimulated T-cell chemotaxisthrough PTK-dependent activation of Vav. Cell Signal. 19: 2351-2360 (2007)

20. Mariggiò S., Filippi B., Iurisci C., Dragani L.K., De Falco V., Santoro M. and Corda D.Cytosolic phospholipase A2α regulates cell growth in RET/PTC-transformed thyroid cells,Cancer Res. 67: 11769-11778 (2007)

21. Colanzi A. and Corda D. Mitosis controls the Golgi and the Golgi controls mitosis. CurrOpin Cell Biol. 4: 386-93 (2007)

22. Berrie C.P., Iurisci C., Piccolo E., Bagnati R., Corda D. Analysis of phosphoinositides andtheir aqueous metabolites. Methods Enzymol. 434:187-232 (2007)

23. Filippi B.M., Mariggiò S., Pulvirenti T., Corda D. Src-dependent signalling regulates actinruffle formation induced by glycerophosphoinositol 4-phosphate. BBA-Mol. Cell. Res.1783:2311-22 (2008)

24. Liberali P., Kakkonen E., Turacchio G., Spaar A., Perinetti G., Böckmann R.A., Corda D.,Colanzi A., Marjomaki V. and Luini A. Macropinosome fission requires plasma membranerecruitment and Pak1-mediated phosphorylation of CtBP1/BARS. EMBO J. 27: 970-981(2008)

25. Dani N., Stilla A., Marchegiani A., Tamburro A., Till S., Ladurner A.G., Corda D. and DiGirolamo M. Combining affinity purification by ADP-ribose-binding macro-domains withmass spectrometry to define the mammalian ADP-ribosyl-proteome. Proc. Natl. Acad. Sci.USA (2009) [Epub ahead of print]

26. Nardini M., Valente C., Ricagno S., Luini A., Corda D., Bolognesi M. CtBP1/BARSGly172->Glu mutant structure: impairing NAD(H) binding and dimerization. Biochem.Biophys. Res. Commun. 381: 70-74 (2009)