SANTA CRUZ BIOTECHNOLOGY, INC.

The Power to Question

c-Fos (4): sc-52

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com

APPLICATIONS

c-Fos (4) is recommended for detection of c-Fos of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1–2 µg per 100–500 µg of total protein (1 mlof cell lysate)], immunofluorescence and immunohistochemistry (includingparaffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry (1 µg per 1 x 106 cells).

Suitable for use as control antibody for c-Fos siRNA (h): sc-29221 and c-FossiRNA (m): sc-29222.

c-Fos (4) X TransCruz antibody is recommended for Gel Supershift and ChIPapplications.

DATA

SELECT PRODUCT CITATIONS

1. Gukovskaya, A.S., et al. 2002. Ethanol metabolism and transcription factor activation in pancreatic acinar cells in rats. Gastroenterology 122:106-118.

2. Budagian, V., et al. 2003. Signaling through P2X7 receptor in human Tcells involves p56lck, MAP kinases, and transcription factors AP-1andNFκB. J. Biol. Chem. 278: 1549-1560.

3. Cloutier, A., et al. 2003. Inflammatory cytokine expression is independentof the c-Jun N-terminal kinase/AP-1 signaling cascade in human neutro-phils. J. Immunol. 171: 3751-3761.

4. Gebhardt, C., et al. 2005. c-Fos-dependent induction of the small Ras-related GTPase Rab11a in skin carcinogenesis. Am. J. Pathol. 167: 243-253.

c-Fos (4): sc-52. Nuclear immunofluorescence stainingof methanol-fixed, phorbol ester-induced HeLa cells.

c-Fos (4)-G: sc-52-G. Western blot analysis of c-Fosexpression in untreated (A,C), phorbol ester-treated(B,D) and EGF-treated (E) A-431 nuclear cell extracts.

c-Fos (4): sc-52 PE. Solid orange histogram representsEGF treated A-431 cells. Green line histogram repre-sents untreated A-431 cells. Pink dotted line representsrabbit IgG PE stained EGF treated A-431 cells.

c-Fos siRNA (h): sc-29221. Western blot analysis of c-Fosexpression in non-transfected control (A) and c-Fos siRNAtransfected (B) A-431 cells. Blot probed with c-Fos (4): sc-52. Actin (I-19): sc-1616 used as specificity and loadingcontrol.

107 K -74 K -

49 K -

36 K -

29 K -

< c-Fos

A B C D E

A B

97 K –

55 K –< c-Fos

55 K –

36 K –

< Actin

BACKGROUND

The v-Fos oncogene was initially detected in two independent murineosteosarcoma virus isolates and an avian nephroblastoma virus. The cellularhomolog, c-Fos, encodes a 62 kDa nuclear phospho-protein that is rapidlyand transiently induced by a variety of agents and functions as a transcrip-tional regulator for several genes. In contrast to c-Jun proteins, which formhomo- and heterodimers which bind to specific DNA response elements, c-Fosproteins are only active as heterodimers with members of the Jun gene fam-ily. Functional homologs of c-Fos include the Fra-1, Fra-2 and Fos B genes. Inaddition, selected ATF/CREB family members can form leucine zipper dimerswith Fos and Jun. Different dimers exhibit differential specificity and affinityfor AP-1 and CRE sites.

REFERENCES

1. Finkel, M.P., et al. 1966. Virus induction of osteosarcomas in mice.Science 151: 698-701.

2. Nishizawa, M., et al. 1987. An avian transforming retrovirus isolated froma nephroblastoma that carries the Fos gene as the oncogene. J. Virol. 61:3733-3740.

CHROMOSOMAL LOCATION

Genetic locus: FOS (human) mapping to 14q24.3; Fos (mouse) mapping to12 D2.

SOURCE

c-Fos (4) is available as either rabbit (sc-52) or goat (sc-52-G) polyclonalaffinity purified antibody raised against a peptide mapping at the N-terminusof c-Fos of human origin.

PRODUCT

Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and0.1% gelatin.

Blocking peptide available for competition studies, sc-52 P, (100 µg peptidein 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).

Available as TransCruz reagent for Gel Supershift and ChIP applications, sc-52 X, 200 µg/0.1 ml.

Available as phycoerythrin conjugate for flow cytometry, sc-52 PE, 100 tests.

Available as HRP conjugate for Western blotting, sc-52 HRP, 200 µg/ml.

Available as fluorescein (sc-52 FITC) or rhodamine (sc-52 TRITC) conjugatesfor use in immunofluorescence, 200 µg/ml.

STORAGE

Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required.

RESEARCH USE

For research use only, not for use in diagnostic procedures.