Saci Short Description And Applications
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Transcript of Saci Short Description And Applications
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Surface Activated Chemical Surface Activated Chemical Ionization – applications to low Ionization – applications to low molecular weight drugs molecular weight drugs analysisanalysis
Simone CristoniSimone Cristoni
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Surface Activated Chemical Ionization
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Surface Activated Chemical Ionization
Controlled, low
potential
Metal surface
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SACI
High sensitivity
High Versatility (different eluent flow rates, compounds and matrix)
Cost effective (avoid pre purification step working by sample dilution)
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Analysis of addictive drugs
Urine
Hair
Saliva
Sample dilution 1:10
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
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Analysis of addictive drugs
Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) Acquisition mode: tandem mass spectrometry (MS/MS) Chromatography column: C18 50x4.1 1.8 µm i.d. Eluents: A:H2O + 0.1% FA %B :CH3CN + 0.1% FA Eluent flow: 500 µL/min Gradient: %B: 5 for 5 minutes, from 5 to 40 % in 10 minutes,
from 40 to 5 % in 2 minutes. Sample volume injected: 20 µL
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Experimental conditions
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Analysis of addictive drugsUrine 2 ng/mL
APCI
SACI
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Analysis of addictive drugsSaliva
4 Retention time (min)
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a) SACI
Sig
nal
in
ten
sity HCT
0
5.3E+5 12.40
LTQ
4 Retention time (min)
14
a) SACI
Sig
nal
in
ten
sity HCT
0
4.1E+4
LTQ
40
Sig
nal
in
ten
sity
3.2E+2
Retention time (min)
14
b) APCI
HCT
LTQ
Sig
nal
in
ten
sity
0
1.89E+2
Retention time (min)
14
c) ESI
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HCT
LTQ
0
Sig
nal
in
ten
sity
5.6E+2
Retention time (min)
14
b) APCI
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HCT LTQ
Sig
nal
in
ten
sity
4.2E+3
Retention time (min)
14
c) ESI
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LTQHCT 12.20
12.30
10 ng/mL of 6-MAM 1 ng/mL of 6-MAM
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Analysis of pesticides
Water Spinach Sample dilution 1:10
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Different matrix
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Analysis of pesticides
Mass spectrometer: ESI-TOF (Bruker Daltonics) Acquisition mode:High mass accuracy full scan MS Chromatography column: C18 50x4.1 3,5 µm i.d. Eluents: A:H2O + 0.1% FA %B :CH3OH + 0.1% FA Eluent flow: 500 µL/min Gradient: %B: 5 for 5 minutes, from 5 to 40 % in 10 minutes,
from 40 to 5 % in 2 minutes. Sample volume injected: 20 µL
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Experimental conditions
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Analysis of pesticideswater
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Analysis of pesticidesspinach
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Analysis of toxin in food
Wine Grape Sample dilution 1:10
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Different matrix
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Analysis of pesticides
Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) Acquisition mode: tandem mass spectrometry (MS3) Chromatography column: C18 250x4 5 µm i.d. Eluents: A:H2O + 0.1% FA %B :CH3CN + 0.1% FA Eluent flow: 1mL/min. Gradient: %B: 5 for 5 minutes, from 5 to 70 % in 10 minutes,
from 70 to 5 % in 2 minutes. Sample volume injected: 20 µL
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Experimental conditions
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Analysis of pesticidesspinach
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Analysis of OTAWine – 10 ng/mL
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Conclusions
High sensitivity (LOD, LOQ, linerity ranges) Cost effecting avoiding matrix effect and pre-concentration step
(e.g. Solid Phase Extraction) Possible to use it in a wide range of chromatographic conditions. High throughput screening of complex mixture if coupled with
high mass accuracy analyzers (e.g TOF or FTICT).
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Future developments
Ion exchange chromatography (low operative voltages employed in SACI makes possible to operate in these chromatographic conditions).
High sensitive quantitation analysis of peptide and protein drugs (ioni monocarica; TOF).
Pesticides (developing high sensitive methods for screening of selected groups of pesticides of interest)
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SACI demo site
Web site: http://www.isbiotechnology.com
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acknowledgment
Dr.ssa Sara Crotti – ISB Milan Dr. Lorenzo Zingaro – ISB Milan Dr.ssa Cristina Canton – ISB Milan Dr.ssa Laura Molin – ISB Milan Dr. Pietro Traldi – CNR Padova Prof. Luigi Rossi Bernardi – University of Milan Dr. Simone Rubini – Bruker Daltonics Dr. Elisa Basso – Bruker Daltonics Dr. Leopoldo Dimiziano – Bruker Daltonics
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www.bdal.com