SUPPLEMENTARY TABLE 1. Location (NAD83) of lakes sampled for this study.

Lake N W

Holly 43°47'31.2" 110°47'54.0"Mica 43°47'02.6" 110°50'33.1"

Solitude 43°47'25.9" 110°50'36.3°

Unnamed Moose 43°55'08.2" 110°49'28.5"

Unnamed Owl 43°57'58.5" 110°50'48.8"

Pass 43°38'38.2" 110°54'30.3"

Marion 43°37'27.9" 110°55'43.4"

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SUPPLEMENTARY TABLE 2. Environmental variables used for principal component comparisons of lakes included in this study and high-elevation stream sites (bold italics) from Tronstad et al. (2016). Stream sites abbreviations include: Death Creek (DCU), Granite Creek (GCU), Cascade Creek (CCU), Owl Creek (OCU) and Moose Creek (MCU). Abbreviations and units follow Table 1.

Site Elevation T %DO DO SPC pH ORP BiofilmHolly 2817 16.5 109 9.3 10.2 7.8 180.1 1Mica 2912 3.2 113 13.1 9.3 8.8 99.9 1Solitude 2769 11.7 122 11.8 9.3 7.6 174.2 2Unnamed Moose Lake 2777 16.1 133 11.6 7.8 8.3 281.2 2Pass 2874 16.6 119 10.2 70 10.2 155 3Marion 2820 17.9 118 9.9 136.4 9 188.5 3CCU 2726 11 115 11.3 9.3 7.9 169.2 1DCU 2697 10.6 109 10.8 190.8 8.62 217.8 2GCU 2703 10.2 130 13 174.3 8.74 216.2 2MCU 2764 17.8 126 10.6 7.8 7.13 300.9 3OCU 2586 5.1 106 12.1 147.5 8.45 211.4 2

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SUPPLEMENTARY FIGURE 1. Photographs of high-elevation lakes sampled in Grand Teton National Park for this study.

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Unnamed Moose Lake Holly Lake

Mica Lake Lake Solitude

Pass Lake Marion Lake

SUPPLEMENTARY FIGURE 2. Photographs of aquatic invertebrates collected in high-elevation lakes of Grand Teton National Park.

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Hesperophylax

Notonecta

Psychoglypha

Apatania

Parameletus

Limnephilidae pupae

Agabus Helobdella stagnalis