S100A8/S100A9

Rela

tive

DIG

E M

EASU

REM

ENTS

F KD FISJIA

APOA1 SAPCRP HP S100A9 SAAS100A8A2M

Q

Figure 4

F KD FISJIA

APOA1 SAPCRP HP SAAA2M

Q

Rela

tive

ELIS

A M

EASU

REM

ENTS

A

Biomarker panelof 7 members

1. A2M

2. APO AI

3. CRP

4. HP

5. S100A8/S100A9

6. SAA

7. SAP

Training setn = 24

12 12

Clinicaldiagnosis F Q

n =

LDA

11 1

1 11

Classified as F

Classified as Q

PercentAgreementwith clinical

diagnosis

91.6% 91.6%+ -

91.6%

Overall

P = 1.0 E -4

Testing setn = 20

10 10

Clinicaldiagnosis F Q

n =

Testing

8 3

2 7

Classified as F

Classified as Q

PercentAgreementwith clinical

diagnosis

80% 70%+ -

75%

Overall

P = 7 E -2

C D

SJIA SJIA

BTraining

SJIA F SJIA Q SJIA F SJIA Q

Testing

Pred

icte

d pr

obab

ilitie

sPatient samples

Figure 5

P*= 9.6E -6 P*=4 E -3

E

CRP : AUC=0.82panel : AUC=0.95

ESR : AUC=0.96

Sen

sitiv

ity

S100A8/S100A9 : AUC=0.73

1- Specificity

CRP : AUC=0.65panel : AUC=0.82

ESR : AUC=0.86

Sen

sitiv

ity

S100A8/S100A9 : AUC=0.78

1- Specificity

Sen

sitiv

ity

CRP : AUC=0.72panel : AUC=0.94

ESR : AUC=0.92S100A8/S100A9 : AUC=0.74

1- Specificity

Training Set Testing Set Training + Testing Set

Figure 5

Training setn = 23

13 10

Clinicaldiagnosis F Q

n =

LDA

10 5

3 5

Classified as F

Classified as Q

PercentAgreementwith clinical

diagnosis

76.9% 50%+ -

65.2%

Overall

P = 0.41

Testing setn = 15

10 5

Clinicaldiagnosis F Q

n =

Testing

3 0

7 5

Classified as F

Classified as Q

PercentAgreementwith clinical

diagnosis

30% 100%+ -

53.3%

Overall

P = 0.20

B C

POLY POLY

D

1- Specificity

CRP : AUC=0.64

POLY F vs. Q

panel : AUC=0.64

Sen

sitiv

ity

ATraining

PF PQ PF PQ

Testing

Pred

icte

d pr

obab

ilitie

s

Patient samples

Supplementary Figure 5

Figure 6

Training setn = 59

22 37

Clinicaldiagnosis

n =

LDA

16 3

6 34

PercentAgreementwith clinical

diagnosis

72.8% 91.9%+ -

84.7%

Overall

P = 4.3E -7

SJIAF

1022 27

SJIAF KD + FI

NOTSJIA F

Classified as SJIA F

Classified as NOT SJIA F

A. Pr

edic

ted

prob

abili

ties

Patient samples

SJIA FKD FI

KD + FI

B

CRP : AUC=0.627panel : AUC=0.801

ESR : AUC=0.661

Sen

sitiv

ity

S100A8/S100A9 : AUC=0.543

1- Specificity

5 10

Clinicaldiagnosis QF QQ

n =

LDA

4 0

1 10

Classified as QF

Classified as QQ

PercentAgreementwith clinical

diagnosis

80% 100%+ -

93.3%

Overall

P = 3.7 E -3

BSJIA

C

CRP : AUC=0.82panel : AUC=0.90

ESR : AUC=0.68

A

Pred

icte

d pr

obab

ilitie

s

Patient samples

QQ QF

Sen

sitiv

ity

1- Specificity

S100A8/S100A9 : AUC=0.74

Figure 7

Figure 8