FIG S1. P. aeruginosa DK2-P24M2-2003 plated on top of LB agar plates and S. aureus JE2

WT supernatant fractionated with a molecular weight cut-off of 10 kDa (A) or supernatant from 3,

6, 17 or 24 hours cultures of S. aureus JE2 (B) applied to wells cut in the agar. Suppression of

‘metallic sheen coverage’ of the P. aeruginosa DK2-P24M2-2003 lawn is observed in a zone

surrounding the fraction of S. aureus JE2 WT supernatant with molecular weight <10 kDa (A), and

the supernatants from 3, 6 and 17 hours S. aureus JE2 WT cultures (B) (indicated by with arrows

and white scale bars).        

 

 

 

 

 

 

TABLE S1. Screening of a sub-collection of S. aureus JE2 mutant strains for their ability to

suppress metallic sheen of a P. aeruginosa DK2-P24M2-2003 lawn

Locus  name   NTML  name  

Gene  symbol  or  description  

Metallic  sheen  suppression  of  DK2-­‐P24M2-­‐2003  lawn  

SAUSA300_0032   NE1868   mecA   +  SAUSA300_0053   NE1846   speG   +  SAUSA300_0064   NE1125   arcD   +  SAUSA300_0065   NE1594   arcA   +  SAUSA300_0099   NE67   plc   +  SAUSA300_0113   NE286   Immunoglobulin  G  binding  

protein  A  precursor  +  

SAUSA300_0114   NE165   Staphylococcal  acessory  regulator  

+  

SAUSA300_0256   NE1438   Holin-­‐like  protein  IrgA   +  SAUSA300_0257   NE1726   Antiholin-­‐like  protein  

LrgB  +  

SAUSA300_0270   NE641   lytM   +  SAUSA300_0380   NE911   ahpC   +  SAUSA300_0489   NE1313   Putative  cell  division  

protein  FtsH  +  

SAUSA300_0509   NE613   ATP  guanido  phosphotransferase  

+  

SAUSA300_0510   NE699   clpC   +  SAUSA300_0511   NE1176   radA   +  SAUSA300_0605   NE1193   sarA   -­‐  SAUSA300_0629   NE679   pbp4   +  SAUSA300_0622   NE731   Putative  membrane  

protein  +  

SAUSA300_0626   NE1374   Teichoic  acid  biosynthesis  protein  B  

+  

SAUSA300_0644   NE467   Conserved  hypothetical  protein  

+  

SAUSA300_0645   NE481   DNA-­‐binding  response  regulator  

+  

SAUSA300_0647   NE645   ABC  transporter,  ATP-­‐binding  protein  

+  

SAUSA300_0648   NE70   ABC  transporter,  permease  protein  

+  

SAUSA300_0684   NE835   fruB   +  SAUSA300_0690   NE1296   saeS   +  SAUSA300_0691   NE1622   saeR   +  SAUSA300_0726   NE629   Glucerat  kinase  family  

protein  +  

SAUSA300_0739   NE1640   lysM  domain-­‐containing   +  

protein  SAUSA300_0752   NE912   clpP   -­‐  SAUSA300_0753   NE1180   Hypothetical  protein   +  SAUSA300_0772   NE543   clfA   +  SAUSA300_0899   NE1315   Adaptor  protein   +  SAUSA300_0955   NE460   atl   +  SAUSA300_1009   NE1694   typA   +  SAUSA300_1014   NE754   pyc   +  SAUSA300_1048   NE808   sdhB   +  SAUSA300_1138   NE569   sucC   +  SAUSA300_1142   NE242   dprA   +  SAUSA300_1147   NE1708   hslU   +  SAUSA300_1178   NE805   recA   +  SAUSA300_1246   NE861   acnA   +  SAUSA300_1255   NE1360   fmtC   +  SAUSA300_1359   NE1920   Polyprenyl  synthetase   +  SAUSA300_1441   NE588   srrB   +  SAUSA300_1442   NE1309   srrA   +  SAUSA300_1512   NE420   pbp3   +  SAUSA300_1514   NE723   fur   +  SAUSA300_1622   NE914   tig   +  SAUSA300_1674   NE1141   Putative  serine  protease  

HtrA  +  

SAUSA300_1708   NE386   rot   +  SAUSA300_1865   NE554   vraR   +  SAUSA300_1867   NE274   Conserved  hypothetical  

protein  +  

SAUSA300_1868   NE907   Hypothetical  Protein   +  SAUSA300_1991   NE873   agrC   -­‐  SAUSA300_1923   NE1948   Autolysin   +  SAUSA300_1989   NE95   agrB   -­‐  SAUSA300_1999   NE1158   rex   +  SAUSA300_2025   NE1607   rsbU   +  SAUSA300_2269   NE550   Conserved  hypothetical  

protein  +  

SAUSA300_2302   NE285   tcaA   +  SAUSA300_2378   NE1010   Hypothetical  protein   +  SAUSA300_2446   NE474   Conserved  hypothetical  

protein  +  

SAUSA300_2467   NE1787   srtA   +  SAUSA300_2479  

 NE1692   cidA   +  

SAUSA300_2493   NE387   Conserved  hypothetical  protein  

+  

SAUSA300_2499   NE1444   crtM   +  SAUSA300_2559   NE1643   DNA-­‐binding  response   +  

   

regulator  SAUSA300_2565   NE391   clfB   +  SAUSA300_2601   NE1167   icaB   +