8/13/2019 Rough Draft Bio 100

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United States Department of Health and Human Services. (n.d.). Retrieved from http:// www.foodsafety.gov/poisoning/causes/index.html

Berkes, J. (n.d.). Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1773546/

Food-poisoning bacteria detection carrier, and method for detecting food-poisoning bacteria

Food consumption is one of the daily task in peoples lives, however, occasionally foodpoisoning will happen. Bacterias are one of the potential causes of food poisoning, otherpotential causes include viruses, parasites, molds, toxins, containments, and allegens.(foodsafetly) There are 5 known families of bacteria which causes food poisoning - Clostridiumdifcile, E. Coli, Bacteroides fragilis, Vibrio cholera, and Clostridium perfringens, and can befurther divided into three categories based on the bacterias mode of infection: cytoskeleton,metalloprotease, and tight junction receptors. Each of the different methods caused the tightfunction within the epithelial cells be disengaged, allowing the bacteria to affect the cell lining.Cytoskeletal bacterias, Clostridium difcult and E. Coli, make changes in the altercation in thecytoskeleton of the epithelial cells. This changes involve actin degradation toxin and a secretion

of skeletal proteins. Metalloprotease bacterias, Bacteroides fragilis and Vibrio cholera, involveszinc-protease affecting the tight junctions permeability, cadherin and occludin respectively.While tight junction bacteria, Clostridium perfringens, involves the cadherin and occludin as thedocking point and removes the claudins as its method of permeability. The bodys response isthe cascade of Cl ions caused by cAMP-gated channels to shift the storage of water from theblood to the epithelial lumen. Understand the cause, which categorical and further whichbacterial family, and the response will help determine systemically prove the causing agent.In conclusion, the proteins and different method require specic DNA, mRNA, and amino acidsequences. With a simple assay which isolate the bacterias DNA and protein sequence, can tellwhether the bacterial is within our sample running a modicated ELIZA and a modied PCR.

With an ELIZA probing the supernatant, after isolating the circular DNA and Proteins

sequence, enable the ability to seek the two key aspect of the bacterias. As these bacterias arewell study, there should be a sequence of each of them, especially the DNA and tRNA sequenceof the proteins. If not, a whole bacterial genome has to be sequenced quickly; the antibodies, ifnot developed, can be hyperdoma cell to have the taqs and receptor. This can be done with aarray to detect the difference. With an Array which has the antibody within the plate. by probingin a fashion where the antibody glows will determinate if the bacteria causes food poisoning.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1773546/