Roles of metabolites in muscle fatigue OBJECTIVES Methodology:Methodology: Experimental...

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Roles of metabolites in muscle fatigue OBJECTIVES • Methodology: • Experimental approach • Temperature • Are the changes in metabolites responsible for the decrease in force during fatigue at the level of the sarcomere

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Transcript of Roles of metabolites in muscle fatigue OBJECTIVES Methodology:Methodology: Experimental...

Page 1: Roles of metabolites in muscle fatigue OBJECTIVES Methodology:Methodology: Experimental approachExperimental approach TemperatureTemperature Are the changes.

Roles of metabolites in muscle fatigue

OBJECTIVES• Methodology:

• Experimental approach• Temperature

• Are the changes in metabolites responsible for the decrease in force during fatigue at the level of the sarcomere

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INTRODUCTION

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ATP PRODUCTION – METABOLIC PATHWAYS

ATP HYDROLYSIS

CREATINE PHOSPHATE

PURINE CYCLE

ADENYLATE KINASE

ATP + H2O ADP + Pi + H+ + WORK

PCr + ADP ATP + Cr

2 ADP ATP + AMP

AMP + H2O + H+ IMP + NH4+

IMP + ASPARTATE + GTP ADENYLSUCCINATE + GDP +Pi

ADENYLSUCCINATE AMP + FUMARATE

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ATP PRODUCTION – METABOLIC PATHWAYS

GLYCOGENOLYSIS AND GLYCOLYSIS

KREB’S CYCLE & OXYDATIVE PHOSPHORYLATION

GLUCOSE + 2 ADP + 2 Pi 2 ATP + 2 PYRUVATE

GLUCOSE + 6 O2 + 36 ADP + 36 Pi 36 ATP + 6 CO2

NO O2

2 LACTATE O2

-OXIDATION

FATTY ACID ACYL-CoA KREB’S CYCLE & OXYDATIVE PHOSPHORYLATION

GLUCOSYL + 3 ADP + 3 Pi 3 ATP + 3 PYRUVATE

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FATIGUE: FAST & GLYCOLYTIC TWITCH MUSCLE

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FATIGUE: SLOW & OXIDATIVE TWITCH MUSCLE

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ATP EFFECT ON MAXIMUM SHORTENING VELOCITY

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ATP EFFECT ON MAXIMUM FORCE

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DISCUSSION

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CONTROLEARLY

FATIGUE FATIGUE

Mg2+ 1 1 1

MgATP 6.18 4.7 4.7

ADPtotal 0.05 0.7 0.7

AMPtotal 0.0002 0.062 0.062

Pitotal 0.88 17.38 17.38

PCr 14.16 1.42 1.42

Creatine 11.46 21.14 47.3

pH 7.00 7.00 6.65

ALL CONCENTRATION IN mM

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Δ DURING FATIGUE

FMAX Ca50

H+ ↑↑ ↓↓ ↓↓Pi ↑↑ ↓↓ ↓↓ATP ↓ ↑ ↓ADP ↑ ↑ ↑AMP ↑ ↑ ↑IMP ↑↑ ↑PCr ↓↓ ↑ ↑Creatine ↑↑ ↓ ↑Lactate ↑↑ → →

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0% CO2

WATER

15% CO2

30% CO2

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Appendix 4: Secondary Metabolites

Appendix 4: Secondary Metabolites

Metabolites, exchanges regulation*

Metabolites, exchanges regulation*

Petroleum Metabolites

Petroleum Metabolites

Secondary metabolites - Napa Valley College · Metabolites Metabolites are all the products of metabolism A primary metabolite is directly involved in normal growth, development,

Secondary metabolites - Napa Valley College · Metabolites Metabolites are all the products of metabolism A primary metabolite is directly involved in normal growth, development,

Caffeine and caffeine metabolites

Caffeine and caffeine metabolites

Longterm Detection of Anabolic Steroid Metabolites in Urinehpst.cz/sites/default/.../5990...metabolites-urine.pdf · Longterm Detection of Anabolic Steroid Metabolites in Urine Abstract

Longterm Detection of Anabolic Steroid Metabolites in Urinehpst.cz/sites/default/.../5990...metabolites-urine.pdf · Longterm Detection of Anabolic Steroid Metabolites in Urine Abstract

Roles of metabolites in muscle fatigue

Roles of metabolites in muscle fatigue

By Angel Moseley Habitats. Contents Page 1.SoundSound 2.LightLight 3.TemperatureTemperature 4.Vegetable patchVegetable patch 5.FieldField 6.HedgerowHedgerow.

By Angel Moseley Habitats. Contents Page 1.SoundSound 2.LightLight 3.TemperatureTemperature 4.Vegetable patchVegetable patch 5.FieldField 6.HedgerowHedgerow.

Managing Fatigue Training Program for Employees. Managing Fatigue For the Employee What is Fatigue Signs of fatigue What causes fatigue Fatigue.

Managing Fatigue Training Program for Employees. Managing Fatigue For the Employee What is Fatigue Signs of fatigue What causes fatigue Fatigue.

Anti metabolites

Anti metabolites

Natural phenolic metabolites with anti-angiogenic ... · Natural phenolic metabolites with anti-angiogenic ... Considering the many secondary natural metabolites available from plants,

Natural phenolic metabolites with anti-angiogenic ... · Natural phenolic metabolites with anti-angiogenic ... Considering the many secondary natural metabolites available from plants,

Secondary metabolites

Secondary metabolites

Secondary metabolites (1)

Secondary metabolites (1)

Plant Secondary Metabolites as Defenses, Regulators, and Primary … · Topical Review Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional

Plant Secondary Metabolites as Defenses, Regulators, and Primary … · Topical Review Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional

OPEN ACCESS metabolites - MDPI

OPEN ACCESS metabolites - MDPI

PRIMARY AND SECONDARY METABOLITES

PRIMARY AND SECONDARY METABOLITES

Metabolites 02 00303

Metabolites 02 00303

Plant Metabolites

Plant Metabolites

Primary metabolites

Primary metabolites

phenolic metabolites

phenolic metabolites