Roles of catalase and glutathione peroxidase in the …10.1007...Supplementary material Roles of...
Transcript of Roles of catalase and glutathione peroxidase in the …10.1007...Supplementary material Roles of...
Supplementary material
Roles of catalase and glutathione peroxidase in the tolerance of a
pulmonate gastropod to anoxia and reoxygenation
Alexis F. Welker1,2, Daniel C. Moreira1 and Marcelo Hermes-Lima1,*
1Laboratório de Radicais Livres, Departamento de Biologia Celular, Universidade de Brasília,
Brasília, DF, 70910-900, Brazil.
2Faculdade da Ceilândia, Universidade de Brasília, Ceilândia, DF, 72220-140, Brazil.
*Corresponding author:
Dr. Marcelo Hermes-Lima
Laboratório de Radicais Livres, Departamento de Biologia Celular, Universidade de Brasília, Brasília,
DF, 70910-900, Brazil.
Phone: +55-61-3107-3103
Email: [email protected] , [email protected] and [email protected]
Fig. S1 Effects of ATZ injection and reoxygenation on total glutathione peroxidase (total-GPX) activity (mU/mg of protein),
glutathione transferase (GST) activity (U/mg of protein) and total glutathione equivalents (GSH-eq) levels (nmol/g of wet
weight) in hepatopancreas (a, b, c) and foot muscle (d, e, f) of Helix aspersa snails. Activity of total GPX (a, d) and GST (b,
e) and GSH-eq levels (c, f) in hepatopancreas and foot muscle from saline- or ATZ-injected snails exposed to anoxia and at
different reoxygenation times “zero” (5 h anoxia), 0.25 h, 0.5 h, 1 h, 2 h and 12 h from saline-injected and ATZ-injected snails.
There were no statistically significant alterations. (n=5–6)
Fig. S2 Effects of anoxia and ATZ injection (a,c) and of reoxygenation (b,d) with catalase inhibition
or not on reduced glutathione (GSH) levels (nmol/g of wet weight) of Helix aspersa snails. GSH
concentration in hepatopancreas (a) and foot muscle (c) from control (Ctrl, no injection), saline- (Sal,
animals injected with 0.9% (w/v) NaCl) or ATZ-injected (ATZ, animals injected with ATZ at 1
mg/gww solubilized in 0.9% NaCl) snails maintained in normoxia or exposed to anoxia. GSH
concentration in hepatopancreas (b) and foot muscle (d) at different reoxygenation times “zero” (5 h
anoxia), 0.25 h, 0.5 h, 1 h, 2 h and 12 h from saline-injected and ATZ-injected snails. There were no
statistically significant alterations. (n=5–6)
Fig. S3 Effects of anoxia and ATZ injection (a,c) and of reoxygenation (b,d) with catalase inhibition
or not on disulfide glutathione (GSSG) levels (nmol/g of wet weight) of Helix aspersa snails. GSSG
concentration in hepatopancreas (a) and foot muscle (c) from control (Ctrl, no injection), saline- (Sal,
animals injected with 0.9% (w/v) NaCl) or ATZ-injected (ATZ, animals injected with ATZ at 1
mg/gww solubilized in 0.9% NaCl) snails maintained in normoxia or exposed to anoxia. GSSG
concentration in hepatopancreas (b) and foot muscle (d) at different reoxygenation times “zero” (5 h
anoxia), 0.25 h, 0.5 h, 1 h, 2 h and 12 h from saline-injected and ATZ-injected snails. There were no
statistically significant alterations. (n=5–6)